Publications

fungi of virgin and cultivated soil of salhiah desert, egypt.	27 species and 13 genera of fungi were identified from virgin and cultivated soil of salhiah. the most abundant species of phosphate solubilizing fungi were aspergillus nidulans, a. niger, a flavus, penicillium lilacinum, p. frequentans and fusarium moniliforme. on cellulose agar the most prevalent species were chaetomium bostrychodes, c. olivaceum, humicola fuscoatra, aspergillus flavus, a. nidulans, a. niger, a. ochraceus, fusarium solani and f. oxysporum. on xylan agar aspergillus fumigatus, ...	1990	2077791
cloning and characterization of a disease resistance response gene in pea inducible by fusarium solani.	disease resistance response genes (drrg) of peas are expressed as the tissue is expressing race-specific or nonhost resistance. a pea genomic clone drrg49-c encompassing one drrg structural gene, the expression of which is correlated with the expression of disease resistance, was sequenced and characterized. the 2.3-kb genomic segment sequenced encompassed 986 bp 5' to the major transcriptional initiation site, a 474-bp open-reading frame interrupted by one 88-bp at-rich intron and an additional ...	1990	2132027
infection with fusarium species in two children with neuroblastoma.	two cases of fusarium infection in children with neuroblastoma are reported. one of the patients had an overwhelming infection and the diagnosis was based on isolation of fusarium moniliforme from blood and skin biopsy, and histological findings. the second patient developed chronic polyarthritis and fusarium solani was cultured from synovial fluid samples taken from two different joints four months apart. no histological documentation of infection was obtained. the response to antifungal therap ...	1990	2148147
cross-reactivity among antigens of different air-borne fungi detected by elisa using five monoclonal antibodies against penicillium notatum.	cross-reactivity among antigens of 12 genera of air-borne fungi, 13 species of penicillium, and 5 species of aspergillus was studied by elisa using five monoclonal antibodies (moabs) against penicillium notatum. epitopes recognized by all the five moabs were susceptible to treatment of mild periodate oxidation and may therefore be associated with carbohydrates. furthermore, our results showed that there is cross-reactivity among antigens of penicillium, aspergillus, and eurotium species. by usin ...	1990	2178052
invasive fusariosis associated with an injury by a stingray barb.	a previously healthy adult male suffered a wound to the dorsal ulnar aspect of his right hand by a stingray barb while fishing off the east coast of florida. two weeks after the imbedded barb had been surgically removed, an erythematous lesion developed around the wound. histopathologic and microbiological studies revealed infection caused by fusarium solani. the patient was successfully treated with debridement and skin grafting in conjunction with ketoconazole therapy.	1990	2213436
crystallization and preliminary x-ray study of a recombinant cutinase from fusarium solani pisi.	recombinant cutinase from fusarium solani pisi is expressed and excreted with very high yields in escherichia coli cultures. cutinase was crystallized at 20 degrees c using the vapour diffusion technique, with polyethylene glycol 6000 as precipitant. best crystals were obtained at ph 7.0 with polyethylene glycol 6000 as precipitant. best crystals were obtained at ph 7.0 with polyethylene glycol at 15 to 20%. they are monoclinic, with space group p2(1) and cell dimensions a = 35.1 a, b = 67.4 a, ...	1990	2213880
naphthoquinone pigments related to fusarubin from the fungus fusarium solani (mart.) sacc.	a review is presented on the naphthoquinone pigments produced by the filamentous fungus fusarium solani (mart.) sacc., and related species. after description of the naphthoquinone structures and biogenesis, the physiological and genetical controls of pigment production are discussed. the biological properties of the main naphthoquinone pigments so far investigated are described. many problems still remain to be resolved in these fields, where nectria haematococca (berk. and br.) wr., the sexual ...	1990	2233399
evaluation of basidiomycete and deuteromycete (fungi imperfecti) extracts for shared allergenic determinants.	aqueous extracts of select members of the basidiomycetes and deuteromycetes (fungi imperfecti) were evaluated for the presence of shared allergenic determinants using skin prick and radio-allergosorbent test (rast) inhibition. twenty adults with perennial symptoms of rhinitis, with or without asthma, were skin-prick tested with six species of deuteromycetes and seven species of basidomycetes. positive weal-and-flare reactivity to pleurotus ostreatus was associated with alternaria alternata, fusa ...	1990	2253084
disseminated infection caused by fusarium solani in a patient with aplastic anemia.		1990	2277679
[antagonism in vitro among phytopathogenic and saprobic fungi from horticultural soils].	two methods were tested in order to determine the existence of in vitro antagonism among saprobic and pathogenic fungi. these microorganisms were the most common isolates from horticultural soils of la plata (buenos aires). trichoderma harzianum; t. koningii and penicillium sp. were antagonistic to all the pathogenic fungi tested, fusarium solani; f. oxysporum; alternaria solani; colletotrichum sp. and sclerotium rolfsii spicaria sp., paecilomyces sp. and chaetomiun sp. were antagonistic only to ...	1990	2287718
sti35, a stress-responsive gene in fusarium spp.	a stress-induced mrna was identified in the phytopathogenic fungus fusarium oxysporum f. sp. cucumerinum. treatment of the fungus with ethanol resulted in the induction of a major mrna species encoding a protein of approximate mr 37,000. a full-length cdna clone of the induced message was obtained. rna blot analysis indicated that the mrna was induced by various other stresses, including treatment with copper(ii) chloride and heat (37 degrees c). however, it was not greatly induced by treatment ...	1990	2376567
antimicrobial activity of naphthoquinones from fusaria.	twenty-two naphthoquinone compounds isolated or derived synthetically from culture extracts of fusarium solani and f. oxysporum were examined for antimicrobial activity. fifteen exhibited antibiotic activity against staphylococcus aureus, and 12 were active against streptococcus pyogenes, but none were active at the highest rate of 128 micrograms/ml against escherichia coli, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, serratia marcescens, or pseudomonas aeruginosa. of 8 plant path ...	1990	2233983
[fusarium solani bronchopneumonia in a patient with acute myeloblastic leukemia].		1990	2098140
mycoflora and nutritional value of shelled melon seeds (citrulus vulgaris schrad.) in nigeria.	thirteen fungi were isolated from mouldy shelled melon seeds. the fungi more frequently isolated included species of mucor (11.25%), rhizopus (13.75%), aspergillus (36.25%), macrophoma (2.50%), penicillium (8.75%), alternaria (5.00%), fusarium (8.75%), botrytis (6.25%), torula (3.75%) and geotrichum (3.75%). healthy, shelled melon seeds inoculated with fusarium solani for 7 or 14 days caused increases in the free fatty acid (f.f.a.) content of the healthy seeds from an initial value of 1.06% to ...	1990	2170965
plant-parasitic nematodes and fungi associated with root rot of peas on prince edward island.	eight commercial pea fields on prince edward island were sampled in june and july over a 2-year period (1986-87) to determine soil population densities and the incidence of nematodes and fungi associated with root rot of peas. root lesion nematodes (pratylenchus spp.) were the dominant endoparasitic nematodes recovered from roots and soil. low populations of the northern root-knot nematode (meloidogyne hapla) were also present. tylenchorhynchus spp. and paratylenchus spp. were recovered frequent ...	1990	19287779
[search for actinomycetes--antagonists of fungi causing sugar beet root rot].	actinomycetes belonging to streptomyces were isolated from the rhizosphere of sugar beet grown on an infected area after cultivation for many years. 44.1 per cent of them proved to be antagonists of phytopathogenic test fungi. the majority of the antagonists were detected among the cinereus. the lowest number of the antagonists was detected among the azureus, the maximum number of the antagonists was observed in the middle and at the end of the sugar beet vegetation period. during various period ...	1989	2534467
fatal fusarium solani infections in baby sharks.	the occurrence of fatal fusariosis in baby bonnethead sharks (sphyrna tiburo) born at the national aquarium, baltimore, maryland, is reported. an atypical strain of fusarium solani was cultured from the tissues of two of the infected sharks following postmortem examination. histopathology revealed an apparent predilection of the fungus for hyaline cartilage. invasion of the cartilage resulted in hyphae with a distorted morphology. in slide culture the fungus displayed the unusual characteristic ...	1989	2746438
antifungal susceptibility of 44 clinical isolates of fusarium species determined by using a broth microdilution method.	the mics and minimum fungicidal concentrations of amphotericin b, natamycin, miconazole, itraconazole, and flucytosine against 17 isolates of fusarium solani, 14 isolates of fusarium moniliforme, 10 isolates of fusarium oxysporum, and 3 isolates of fusarium semitectum were determined by a broth microdilution method. amphotericin b and natamycin were the most active agents tested and failed to show any inoculum size effect. in contrast, miconazole and itraconazole showed poor inhibitory and fungi ...	1989	2817867
molecular transformation of fusarium solani with an antibiotic resistance marker having no fungal dna homology.	a vector was constructed for transformation of the plant pathogenic fungus fusarium solani. the promoter 35sp, from cauliflower mosaic virus, was fused to the bacterial gene aph(3')ii, which confers resistance to the aminoglycoside antibiotic g418. two transformation procedures were developed: one using isolated fungal protoplasts, the other using germinated fungal spores. a transformation frequency of 3.3 g418-resistant colonies were obtained per microgram dna. of 14 colonies analyzed, 12 had v ...	1989	2550150
fusariosis in the shrimp penaeus semisulcatus cultured in israel.	the first case of a mycotic infection in shrimp in israel is reported. fusarium solani produced a large melanized lesion in a specimen of penaeus semisulcatus cultured at eilat, on the red sea. fungal hyphae elicited a strong hemocytic response in cuticular and connective tissues. in the underlying muscle, the inflammatory reaction appeared weaker, suggesting a gradual failure by the host to resist mycelial invasion. three cases of human keratomycosis by f. solani have been reported in recent ye ...	1989	2594048
characterization of the termini of linear plasmids from nectria haematococca and their use in construction of an autonomously replicating transformation vector.	the mitochondria of isolate fs37 from nectria haematococca mating population i (fusarium solani f. sp. cucurbitae) contain two linear plasmids, pfsc1 and pfsc2, of 9.2 and 8.3 kbp, respectively. evidence for a protein blocking the 5' termini of these plasmids was obtained from exonuclease digestion experiments. a single protein band with an apparent mr of 80 k was labeled when the dna-protein complex of either plasmid was reacted with [125i] bolton-hunter reagent and then digested with dnase i. ...	1989	2598274
potted plants in hospitals as reservoirs of pathogenic fungi.	the soils of five potted plants cultivated within a hospital were investigated for the presence of fungal opportunistic pathogens of humans. a total of 16 potentially pathogenic species were isolated, including aspergillus fumigatus at up to 53.5 colony-forming units (cfu) per gram dry soil and scedosporium apiospermum (pseudallescheria boydii) at up to 97.0 cfu/g. other common species included phialophora verrucosa and fusarium solani. scedosporium inflatum, a recently described emerging pathog ...	1989	2671744
structure of the cutinase gene and detection of promoter activity in the 5'-flanking region by fungal transformation.	the cutinase gene from fusarium solani f. sp. pisi (nectria hematococa) was cloned and sequenced. sau3a fragments of genomic dna from the fungus were cloned in a lambda charon 35 vector. when restriction fragments generated from the inserts were screened with 5' and 3' probes from cutinase cdna, a 5.5-kilobase ssti fragment hybridized with both probes, suggesting the presence of the entire cutinase gene. a 2,818-base pair segment was sequenced, revealing a 690-nucleotide open reading frame that ...	1989	2703464
fusarium oxysporum keratitis.	we describe herein a patient with a rare occurrence of mycotic keratitis caused by fusarium oxysporum. the fungus destroyed the cornea, and a perforating corneal graft had to be performed. the drug of choice in such diagnosed cases is natamycin. a comparison between fusarium oxysporum and fusarium solani is discussed.	1989	2919034
diagnosis and successful treatment of fusariosis in the compromised host.	we present six cases of fusariosis caused by fusarium solani that were diagnosed over a three-year period in 166 adult patients undergoing chemotherapy for acute leukemia. necrotic skin lesions were evident in four patients, fungemia in three, and a deep cellulitis around a great toe nail at the time of a febrile illness in two. the mean minimal inhibitory concentration (mic) of amphotericin b was 3.3 micrograms/ml and of miconazole, 5.3 micrograms/ml; all isolates were resistant to 5-fluorocyto ...	1988	3053920
purification and characterization of cutinase from a fluorescent pseudomonas putida bacterial strain isolated from phyllosphere.	cutinase, an extracellular enzyme, was induced by cutin in a fluorescent pseudomonas putida strain that was found to be cohabiting with an apparently nitrogen-fixing corynebacterium. this enzyme was purified from the culture fluid by acetone precipitation followed by chromatography on deae-cellulose, qae-sephadex, sepharose 6b, and sephadex g-100. the purified enzyme showed a single band when subjected to polyacrylamide electrophoresis and the enzymatic activity coincided with the protein band. ...	1988	3130804
invasive fusarium solani infections in patients with acute leukemia.	two cases of disseminated hyalohyphomycosis due to fusarium solani in patients with acute nonlymphocytic leukemia were studied. the clinical features in both patients included fever, fungemia, severe myalgias, disseminated ecthyma gangrenosum-like skin lesions, ocular symptoms, and a fatal outcome despite systemic administration of amphotericin b in the setting of profound, persistent granulocytopenia. in vitro studies showed the resistance of both f. solani isolates to amphotericin b, 5-fluoroc ...	1988	3164881
[fractional and fatty acid composition of the lipids in the fungus fusarium solani and its oligoconidial mutants].		1988	3185313
fusarium solani osteomyelitis with probable nosocomial spread.	a case of fusarium solani osteomyelitis is reported in a previously healthy adult male who received multiple injuries during an automobile accident. combined histologic and mycologic data proved it to be a case of hyalohyphomycosis resulting from fusarium. to the best of the authors' knowledge, this is the first reported case of osteomyelitis resulting from f. solani. a concurrent case of saprophytic wound colonization by the same organism is reported in another patient in the same ward who had ...	1988	3195503
[endophthalmitis caused by the microscopic fungus fusarium solani].		1988	3197144
disseminated fusarium solani infection with cutaneous nodules in a bone marrow transplant patient.	fusarium is a ubiquitous fungus that commonly colonizes ulcerated, burned, or traumatized skin and may cause keratitis and onychomycosis in healthy hosts. serious disseminated infection due to fusarium has been reported with increasing frequency in immunocompromised patients. we describe a bone marrow transplant patient who developed fungal septicemia and disseminated skin nodules due to fusarium solani. fusarium should be recognized as a potential cause of deep fungal infection in immunocomprom ...	1988	3069758
mycoflora of air-conditioners dust from riyadh, saudi arabia.	using the hair baiting technique, 6 genera and 14 species were collected on sabouraud's dextrose agar from 37 dust samples from air-conditioners. the most common fungi were chrysosporium tropicum, c. indicum, c. keratinophilum, aspergillus flavus followed by acremonium strictum and scopulariopsis brevicaulis. using the dilution-plate method, 26 genera and 52 species were collected from 37 dust samples on glucose-(23 genera and 45 species) and cellulose-(18 genera and 34 species) czapek's agar at ...	1988	3236219
acute bovine pulmonary emphysema caused by the fungus fusarium semitectum.	acute bovine pulmonary emphysema (abpe) is known to occur throughout the world. the documented causes include 3-methylindole, 4-ipomeanol and perilla ketone. although 4-ipomeanol is a phytoallexin from fusarium solani, this is the first reported incidence of toxicity involving fusarium semitectum. this report describes the poisoning of cattle consuming fusarium semitectum contaminated pink half-runner bean refuse.	1988	3388752
fungal keratitis in contact lens wearers.	in a retrospective review from 1972 through 1987 of patients with microbial keratitis, fungal infection occurred in four (4%) of 90 cosmetic or aphakic contact lens wearers and in four (27%) of 15 patients using a therapeutic soft contact lens. predisposing factors included improper lens care by the refractive lens wearers and a chronic epithelial defect with topical corticosteroid use among the therapeutic lens wearers. the responsible organisms in the refractive lens group were fusarium solani ...	1988	3195650
two linear plasmids in mitochondria of fusarium solani f. sp. cucurbitae.	two linear plasmid-like dnas designated pfsc1 (9.2 kbp) and pfsc2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus fusarium solani f. sp. cucurbitae race 1. the plasmids were maternally inherited and copurified with mitochondrial dna obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. the plasmids did not share extensive sequence similarity. no homology was detected between either plasmid and the nuclear or mitochondrial genome w ...	1988	3399553
antifungal hydrolases in pea tissue : i. purification and characterization of two chitinases and two beta-1,3-glucanases differentially regulated during development and in response to fungal infection.	chitinase and beta-1,-3-glucanase activities increased coordinately in pea (pisum sativum l. cv "dot") pods during development and maturation and when immature pea pods were inoculated with compatible or incompatible strains of fusarium solani or wounded or treated with chitosan or ethylene. up to five major soluble, basic proteins accumulated in stressed immature pods and in maturing untreated pods. after separation of these proteins by chromatofocusing, an enzymic function could be assigned to ...	1988	16666142
antifungal hydrolases in pea tissue : ii. inhibition of fungal growth by combinations of chitinase and beta-1,3-glucanase.	chitinase and beta-1,3-glucanase purified from pea pods acted synergistically in the degradation of fungal cell walls. the antifungal potential of the two enzymes was studied directly by adding protein preparations to paper discs placed on agar plates containing germinated fungal spores. protein extracts from pea pods infected with fusarium solani f.sp. phaseoli, which contained high activities of chitinase and beta-1,3-glucanase, inhibited growth of 15 out of 18 fungi tested. protein extracts f ...	1988	16666407
granulomatous peritonitis in fish associated with fusarium solani.		1987	3438995
direct examination vs culture of biopsy specimens for the diagnosis of keratomycosis.	in two patients with fungal keratitis, direct examination of corneal biopsy specimens showed positive fungal elements, but cultures of biopsy specimens failed to disclose fungal growth. we compared the value of direct examination and culture of biopsy specimens in the diagnosis of keratomycosis in rabbits with experimental fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. cultures disclosed seven specimens (70%) positive for candida and eight (80%) for fusa ...	1987	3578461
enzymatic hydrolysis of diethylpyrocarbonate, a commonly used histidine modifying agent, by esterases.	diethylpyrocarbonate, a reagent commonly used to modify active site histidines in enzymes, was found to be hydrolyzed by several esterases. two of these, cutinase, a typical serine esterase from the fungus fusarium solani pisi, and thioesterase b from the uropygial gland of the mallard duck anus platyrhynchus, hydrolyzed diethylpyrocarbonate so rapidly that histidine modification could not be detected except when the enzymic activity was inhibited by diisopropylfluorophosphate treatment or by th ...	1987	3595987
fast method for two-dimensional electrophoresis of proteins from biological samples.	the method for two-dimensional gel electrophoresis of j. klose and m. feller [(1981) electrophoresis 2, 12-24] has been simplified by reducing the thickness of the gels from 3.5 to 1.1 mm for isoelectric focusing gels and from 3.5 to 0.84 mm for sodium dodecyl sulfate slab gels. thin gels need less reagents and smaller sample volumes. cooling of the thin gels during electrophoresis is more effective, which allows the use of higher electric power. therefore, less time is required for an electroph ...	1987	3674367
disseminated cutaneous fusarium infection with vascular invasion in a leukemic patient.	a 61-year-old male with leukemia manifested multiple cutaneous nodules on his whole body surface, subcutaneous nodules on his arms and a tongue tumor. septate hyphae were observed microscopically in scrapings from the surfaces of the cutaneous lesions. fusarium solani and f. anthophilum were isolated from scrapings from the several skin lesions. histological examination revealed the presence of numerous septate hyphae in the lumina of vessels in the dermis. the fungal elements in the cutaneous t ...	1987	3302198
pectate lyase from fusarium solani f. sp. pisi: purification, characterization, in vitro translation of the mrna, and involvement in pathogenicity.	since indirect experimental evidence suggested that penetration of fusarium solani f. sp. pisi into its host (pisum sativum) involved pectin-degrading enzymes (w. köller, c. r. allan, and p. e. kolattukudy (1982) physiol, plant pathol. 20, 47-60), direct tests were made for the production of such degradative enzymes by this pathogen. when the organism was grown on pectin, a pectate lyase (ec 4.2.2.2) was released into the media. this lyase was purified to apparent homogeneity from the culture fi ...	1987	3310898
microbiological diagnosis of suppurative keratitis in bangladesh.	experience in setting up an inexpensive microbiology laboratory in the bangladesh national society for the blind eye hospital and training complex at chittagong is presented, together with the results of a pilot study to identify organisms responsible in 33 consecutive cases of suppurative keratitis in the chittagong area of bangladesh. of the 33 cases 21 were positively identified by means of gram stain and/or culture. two-thirds of the responsible organisms were bacteria, and one-third were fu ...	1987	3555609
the value of calcofluor white in the diagnosis of mycotic and acanthamoeba infections of the eye and ocular adnexa.	in search of an easy and quick method for the diagnosis of fungal and acanthamoeba infections of the eye, the authors have used calcofluor white (cfw), a fluorescent brightener with marked affinity for chitin and cellulose which are present in the cell walls of fungi and cysts of acanthamoeba. paraffin-embedded tissues containing mycotic organisms were stained with cfw and examined under a fluorescent microscope. several fungi such as candida sp, aspergillus sp, phycomycetes, fusarium solani, hi ...	1987	2436125
antibiotic activity of the pyrenocines.	pyrenocine a, a phytotoxin produced by pyrenochaeta terrestris (hansen) gorenz, walker and larson, possesses general antibiotic activity against plants, fungi, and bacteria. effective doses for 50% inhibition (ed50s) are 4 micrograms/ml for onion seedling elongation; 14, 20, 20, and 25 micrograms/ml for the germination of asexual spores of fusarium oxysporum f. sp. cepae, fusarium solani f. sp. pisi, mucor hiemalis, and rhizopus stolonifer, respectively. pyrenocine a also inhibits the linear myc ...	1987	3594310
fusarium solani: evidence for common antigenic/allergenic determinants with other fungi imperfecti.	aqueous extracts of fusarium solani and other members of the fungi imperfecti were evaluated for the presence of common antigenic/allergenic determinants using skin-prick testing, radio-allergo-sorbent test (rast) inhibition, and immunoelectrophoretic methods. prevalence of skin reactivity in forty-four atopic individuals, tested with commercially available fungal extracts, ranged from 27.3% for alternaria tenuis to 6.8% for penicillium notatum. no specific patterns of reactivity emerged from st ...	1987	2438068
cutaneous infection caused by fusarium solani and fusarium oxysporum.		1987	3325538
pea genes associated with non-host disease resistance to fusarium are also active in race-specific disease resistance to pseudomonas.	a given plant species is able to resist most of the potentially pathogenic microorganisms with which it comes in contact. this phenomenon, known as non-host resistance, can be overcome only by a very small number of 'true pathogens' which can use that plant as a host. in some cases, plants have developed mechanisms for overcoming infection by specific races or strains of a true pathogen. this race-specific resistance can be easily manipulated into agronomically important cultivars by plant breed ...	1987	24301193
isolation of a fusarium solani mutant reduced in cutinase activity and virulence.	fusarium solani isolate t-8 produces an extracellular enzyme, cutinase, which catalyzes the degradation of cutin in the plant cuticle. cutinase activity can be measured by the hydrolysis of either the artifical substrate, p-nitrophenylbutyrate (pnb), or radioactive cutin containing [14c]palmitic acid. in the present study, the culture filtrate contained basal levels of cutinase when t-8 was grown on acetate as a sole source of carbon. after mutagenesis, a cutinase-defective mutant (pnb-1) was id ...	1986	3782031
high field and 2d-nmr studies with the aporphine alkaloid glaucine.	the aporphine alkaloid glaucine (1) was examined by comparison of the high field (600 mhz) 1h-nmr spectra of 1 vs. racemic 6a,7,7-trideutereoglaucine (4,5), by computer-simulated 1h-nmr spectra at 600 mhz, by using decoupled proton spectra, and two-dimensional cosy and hetcor experiments with 1 at 500 and 360 mhz, respectively, and using high field (90 mhz) 13c-nmr of s-(+)-glaucine (1). emphasis was placed on the resolution of the chemical shifts and coupling constants for the h-4 alpha, h-4 be ...	1986	3783155
the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases.	two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ...	1986	3707549
fusarium solani: prevalence of skin reactivity and antigenic allergenic analysis.	the prevalence of fusarium solani reactivity in atopic individuals with symptoms of mold allergy was assessed with skin test and rast. in addition, f. solani preparations were evaluated for antigenic/allergenic activity. atopic individuals tested, 24.5% (n = 69), had positive skin reactions to a phosphate-buffered saline extract of f. solani, and these responses were statistically correlated with rast results. immunoelectrophoretic techniques demonstrated that this extract was antigenic in rabbi ...	1986	3711551
the pathogenicities of cylindrocarpon tonkinense and fusarium solani in the rabbit cornea.	the pathogenicity of cylindrocarpon tonkinense in the cornea was evaluated and compared with that of fusarium solani in rabbits. f. solani was inoculated into the right eyes of 14 rabbits and c. tonkinense was into the left eyes of same rabbits. the corneal lesions of both eyes were examined carefully by slit lamp every day for three weeks and the severity of infections were compared each other. for histopathologic study, several eyes were enucleated periodically. c. tonkinense has a pathogenici ...	1986	3762655
disseminated hyalohyphomycosis in a leukemic patient.	a 69-year-old man with acute myeloblastic leukemia was admitted to the hospital for induction chemotherapy. a complication caused by a disseminated mycotic infection occurred while the neutropenic patient was maintained on a course of cytotoxic drugs, antibiotics, and corticosteroid therapy. the causal isolate was identified as fusarium solani on the basis of its colonial and microscopic morphology. this fungus developed in the patient's tissue in the form of hyaline, branched, septate hyphae. t ...	1986	3464232
corneal biopsy in the diagnosis of keratomycosis.	in two patients, a 55-year-old man and a 49-year-old man, who had fungal keratitis initially undiagnosed by corneal scrapings the condition was successfully diagnosed by corneal biopsy. we compared corneal biopsy specimens and corneal scraping in the diagnosis of keratomycosis in rabbits with experimental bilateral fungal keratitis caused by fusarium solani, aspergillus fumigatus, and candida albicans. corneal scrapings disclosed three specimens (30%) positive for candida, five (50%) for fusariu ...	1986	3485380
rapid visualization of three common fungi using fluorescein-conjugated lectins.	the feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. using a panel of fluorescein-conjugated lectins, candida albicans, aspergillus fumigatus, and fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue samples and fixed histopathologic specimens taken from experimental mycoses. additionally, aspergillus and fusarium were consistently differenti ...	1986	3514531
comparison of the pathogenicities of fusarium solani and candida albicans in the rabbit cornea.	the pathogenicity of f. solani was compared with that of candida albicans in experimental keratitis in rabbits. f. solani was inoculated into the right eye of each rabbit, and c. albicans into the left eye. the corneal lesions in both eyes were examined by slit lamp every day for 3 weeks and the severity of infections compared clinically and histopathologically. f. solani produced significantly more severe clinical infection in the cornea, compared to c. albicans. histopathologically, f. solani ...	1986	3537261
fungemia due to fusarium solani in an immunocompromised child.		1986	3523454
mechanism by which contact with plant cuticle triggers cutinase gene expression in the spores of fusarium solani f. sp. pisi.	spores of the phytopathogenic fungus fusarium solani f. sp. pisi were shown to produce the extracellular enzyme, cutinase, only when cutin or cutin hydrolysate was added to the spore suspension. dihydroxy-c(16) acid and trihydroxy-c(18) acid, which are unique cutin monomers, showed the greatest cutinase-inducing activity. experiments with several compounds structurally related to these fatty acids suggested that both a omega-hydroxyl and a midchain hydroxyl are required for cutinase-inducing act ...	1986	16593666
association of criconemella xenoplax and fusarium spp. with root necrosis and growth of peach.	criconemella xenoplax, fusarium solani, and f. oxysporum caused necrosis of nemaguard peach feeder roots in greenhouse tests. root necrosis was more extensive in the presence of either fungus than wtih c. xenoplax alone. shoot growth and plant height were less for plants inoculated with f. oxysporum or f. solani than for plants inoculated with the fungi plus c. xenoplax. neither synergistic nor additive effects on root necrosis or plant growth occurred between c. xenoplax and the fungal pathogen ...	1986	19294169
the use of a hydrophobic resin as a product reservoir in steroid transformations.	particles of the hydrophobic resin polydimethylsiloxane were found to preferentially accumulate steriods on the basis of their hydrophobicity. thus, the resin selectively sorped the steroid products resulting from the transformation of diosgenin by nocardia rhodochrous, with the result that higher yields of the later biotransformation product, 1-dehydrodiosgenone, and lower yields of the first product, diosgenone, were obtained than in the absence of resin. furthermore, steroids accumulated by t ...	1985	18553742
calcofluor and ink-potassium hydroxide preparations for identifying fungi.	calcofluor and ink-potassium hydroxide preparations identified fusarium solani, aspergillus fumigatus, and candida albicans, the three most common ocular fungal pathogens, in scrapings, biopsy specimens, and tissue sections of corneal mycotic infections in rabbits. these stains also identified fungal organisms in specimens from four human patients with keratomycoses. neither procedure requires more than a few minutes to perform or extensive training or experience to interpret. the specimen stain ...	1985	4061554
anterior segment resection because of fusarium solani keratitis and endophthalmitis.		1984	6610405
formation of trichothecenes by fusarium solani var. coeruleum and fusarium sambucinum in potatoes.	fusarium solani var. coeruleum can form deoxynivalenol in potato tubers and in liquid medium, although concentrations observed in the rot were highly variable; acetyldeoxynivalenol and ht-2 toxin were detected in 1 to 3 tubers only (of 57). trichothecenes were also detected in a very few (3 of 20) cultures of fusarium sambucinum in potato tubers.	1984	6742832
recurrent fusarium keratomycosis: a light and electron microscopic study.	corneal buttons infected with fusarium solani, were removed during penetrating keratoplasty, and studied by light and electron microscopy. histologically, it appeared that the hyphae penetrated descement 's membrane and the posterior stroma from the anterior chamber. their cell wall, plasmalemma, and cytoplasm were preserved indicating that the fungi might be still active despite antifungal therapy, which may not be effective once the fungus penetrates descemet's membrane. the other interesting ...	1984	6372614
ethylene: symptom, not signal for the induction of chitinase and beta-1,3-glucanase in pea pods by pathogens and elicitors.	infection of immature pea pods with fusarium solani f.sp. phaseoli (a non-pathogen of peas) or f.sp. pisi (a pea pathogen) resulted in induction of chitinase and beta-1,3-glucanase. within 30 hours, activities of the two enzymes increased 9-fold and 4-fold, respectively. chitinase and beta-1,3-glucanase were also induced by autoclaved spores of the two f. solani strains and by the known elicitors of phytoalexins in pea pods, cadmium ions, actinomycin d, and chitosan. furthermore, exogenously app ...	1984	16663892
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.	we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...	1983	6195179
relative efficacy of miconazole against rhizoctonia sp., paecilomyces lilacinus and fusarium solani.		1983	6618536
studies on the antigenic composition of aqueous cotton dust extracts.	an immune response to cotton dust components has been proposed as a possible mechanism of byssinosis. to evaluate this possibility, specific precipitating antibodies against aqueous cotton dust extracts (acde) were raised in rabbits, and a reference pattern for crossed immunoelectrophoresis (cie) was established using this extract. a significant portion of the immune response was directed against alternaria tenuis, aspergillus niger, and fusarium solani, all documented contaminants of cotton dus ...	1983	6642705
septic arthritis due to fusarium solani.	a case of fusarium arthritis is reported. fusarium, a mold ubiquitous in soil and on plants commonly causes keratomycosis and infects burns. recent reports demonstrate that fusarium may produce serious visceral infection in compromised hosts. disseminated infection has been universally fatal. in contrast, our case and several others with localized visceral infection were cured by intravenous amphotericin b and drainage. although fusarium grows readily on mycologic media, blood and other cultures ...	1983	6842474
[antifungal and antimicrobial activity of beta-ionone and vitamin a derivatives].	the antifungal and antimicrobic activity of some derivatives of beta-ionon and vitamin a was studied. these compounds (citral, pseudo-ionon. beta-ionon aldehyde c14, ketone c18 and its derivatives--4,18-diketone, alcohol c18, semicarbazide ketone c18), as well as vitamin a and its derivatives--retinal, acetate, retinoic acid--differ in composition, structure and substituents of c-atoms in beta-ionon ring and in polyenoic chain. fusarium solani, botrytis cenerea and verticillum dahliae ii, race 4 ...	1983	6364128
chitinase in bean leaves: induction by ethylene, purification, properties, and possible function.	ethylene induced an endochitinase in primary leaves of phaseolus vulgaris l. the enzyme formed chitobiose and higher chitin oligosaccharides from insoluble, colloidal or regenerated chitin. less than 5% of the total chitinolytic activity was detected in an exochitinase assay proposed by abeles et al. (1970, plant physiol. 47, 129-134) for ethylene-induced chitinase. in ethylene-treated plants, chitinase activity started to increase after a lag of 6 h and was induced 30 fold within 24 h. exogenou ...	1983	24263941
ovicidal fungi in soils of cuba.	thirty-six strains of ovicidal fungi were isolated from 122 investigated soil samples collected in cuba. the isolated strains belong to 8 genera, 2 strains consist of sterile mycelium only. most of the strains belong to the genus fusarium (4 species, 1 undetermined strain), the remaining ones to mortierella (3 species and 6 undetermined strains), humicola (2 species), paecilomyces (2 species) and penicillium (2 species). the genera verticillium, gliocladium and cunninghamella were represented by ...	1982	6890012
studies on the etiology and symptomatology of root and storage rot disease of cocoyam in nigeria.	the losses caused by root and storage rot of cocoyam in nigeria are estimated as 40 to 45%. field symptoms of the disease include inhibited growth, leaf chlorosis followed by necrosis and shrivelling of affected parts, and finally premature death of the aerial portions of the plant. a large proportion of the roots are destroyed. poor production of cormels and reduced corm size are other field symptoms of the disease, differing according to the type of causal agent. botryodiplodia theobromae, fus ...	1982	6891209
mechanism of action of cutinase: chemical modification of the catalytic triad characteristic for serine hydrolases.	cutinase from fusarium solani f. sp. pisi was inhibited by diisopropyl fluorophosphate and phenylboronic acid, indicating the involvement of an active serine residue in enzyme catalysis. quantitation of the number of phosphorylated serines showed that modification of one residue resulted in complete loss of enzyme activity. one essential histidine residue was modified with diethyl pyrocarbonate. this residue was buried in native cutinase and became accessible to chemical modification only after ...	1982	6809046
the immunosuppressive effects of trichothecenes and cyclochlorotine on the antibody responses in guinea pigs.	immunosuppressive effects of trichothecenes of fusarium solani and fusarium nivale, t-2 toxin and fusarenon-x, and also of a mycotoxin of penicillium islandicum, cyclochlorotine, were studied by measuring the anti-2,4-dinitrophenyl (dnp) antibody responses in guinea pigs immunized with dnp-bovine serum albumin. among these mycotoxins, t-2 toxin alone suppressed strongly the anti-dnp antibody responses at a certain sublethal dose. with other mycotoxins, no effect was observed at any sublethal dos ...	1982	7120038
fusarium solani keratitis.		1982	7169026
interference in the gas chromatographic determination of deoxynivalenol in cultures of fusarium solani on corn.		1982	7182412
[perforatory keratoplasty for keratitis due to fusarium solani].		1981	7024056
mechanics of chromosome separation during mitosis in fusarium (fungi imperfecti): new evidence from ultrastructural and laser microbeam experiments.	the anaphase-telophase spindle usually elongates, and it has been assumed that the spindle pushes the incipient daughter nuclei apart. to test this assumption, we used a laser microbeam to sever the central spindle of the fungus, fusarium solani, and measured the rate of separation of incipient daughter nuclei. when the microbeam was aimed beside the spindle separation occurred at a rate (8.6 micrometer/min) that did not differ significantly from the rate (7.6 micrometer/m) in unirradiated cells ...	1981	7309791
fusarium solani keratitis. first report from malaysia.		1981	7343825
comparative effect of amphotericin b on rhizoctonia sp., paecilomyces lilacinus & fusarium solani in vitro & in experimental keratitis.		1981	6268532
localization of fungal components in the pea-fusarium interaction detected immunochemically with anti-chitosan and anti-fungal cell wall antisera.	antisera specific for purified cell walls of fusarium solani f. sp. pisi and phaseoli and of shrimp shell chitosan were utilized as immunochemical probes to determine the location of fungal components in the pea-fusarium interaction.within 15 minutes after inoculation, fungal cell wall components appear to enter the plant cell and to accumulate inside the plant cell wall as fungal growth on the plant tissue is inhibited. the accumulation patterns of chitosan and all components containing hexosam ...	1981	16661621
effects of light and of fusarium solani on synthesis and activity of phenylalanine ammonia-lyase in peas.	phenylalanine ammonia-lyase was purified from peas, and a specific antiserum against the enzyme was produced in rabbits. the antiserum was used to study the first 8 hours of the phenylalanine ammonia-lyase activity response in two different organs of the pea from different developmental stages and in response to two different stimuli. etiolated seedlings were pulse-labeled with l-[(35)s]methionine after either no light exposure or after specific periods of irradiation with blue light. immature p ...	1981	16661979
microbiological systems in organic synthesis: preparative-scale resolution of (rs)-glaucine by fusarium solani and stereospecific oxidation of (r)-(-)-glaucine by aspergillus flavipes.	the destructive resolution of (6ar,s)-glaucine (ic) was accomplished by oxidation of the (6as)-(+)-enantiomer (ia), using fusarium solani atcc 12823 to yield the unnatural alkaloid (6ar)-(-)-glaucine (ib). eighteen cultures were examined for their ability to metabolize the (6ar)-(-)-enantiomer (ib), and aspergillus flavipes atcc 1030 was found to catalyze the stereoselective oxidation of this substrate to didehydroglaucine. thus, it has been demonstrated that "r" and "s" organisms exist with reg ...	1981	16345776
degradation of c-labeled lignins and c-labeled aromatic acids by fusarium solani.	abilities of isolate af-w1 of fusarium solani to degrade the side chain and the ring structure of synthetic dehydrogenative polymerizates, aromatic acids, or lignin in sound wood were investigated under several conditions of growth substrate or basal medium and ph. significant transformations of lignins occurred in 50 days in both unextracted and extracted sound wood substrates with 3% malt as the growth substrate and the ph buffered initially at 4.0 with 2,2-dimethylsuccinate. degradation of li ...	1980	16345616
glycosidic enzyme activity in pea tissue and pea-fusarium solani interactions.	membrane barriers which prevent direct contact between fusarium solani and pea endocarp tissue prevent fungal spores from inducing phytoalexin production. conversely, preinduced host resistance responses are not readily transported from the plant across the membrane barrier to fusarium macroconidia.crude enzyme extracts from pea endocarp tissues partially degrade fusarium solani f. sp. phaseoli cell walls. activities of the glycosidic enzymes, chitinase, beta-1,3-glucanase, chitosanase, beta-d-n ...	1980	16661404
chitosan as a component of pea-fusarium solani interactions.	chitosan, a polymer of beta-1,4-linked glucosamine residues with a strong affinity for dna, was implicated in the pea pod-fusarium solani interaction as an elicitor of phytoalexin production, an inhibitor of fungal growth and a chemical which can protect pea tissue from infection by f. solani f. sp. pisi. purified fusarium fungal cell walls can elicit phytoalexin production in pea pod tissue. enzymes from acetone powders of pea tissue release eliciting components from the f. solani f. sp. phaseo ...	1980	16661405
degradation of humic acids, extracted from manure and soil by some streptomycetes and fungi.	humate-decomposing microorganisms were isolated from alluvial soil, farmyard manure and peat by direct plating and enrichment techniques. peat was found to harbour more of these organisms than soil and manure. out of 21 actinomycetes and 6 fungi 9 and 3 isolates, respectively, were found to utilize manure-humate as a sole source of carbon and to a less extent as a source of both carbon and nitrogen in liquid medium. these efficient cultures were identified as streptomyces spec. (a 11 and a 12). ...	1980	6448526
structural studies on cutinase, a glycoprotein containing novel amino acids and glucuronic acid amide at the n terminus.	cutinase i and cutinase ii, two extracellular enzymes produced by fusarium solani pisi, were shown to be glycoproteins containing 4.3% and 5.1% carbohydrates, respectively. upon treatment with alkali both enzymes generated chromophores which absorbed at 241 nm. treatment of both proteins with alkaline nab3h4 gave labeled protein and labeled monosaccharides. hydrolysis of the labeled protein followed by chromatographic and enzymatic analyses of the products showed that alanine, 2-aminobutyrate, p ...	1980	7398618
fusarium solani endophthalmitis.		1980	6966137
ecological distribution of fusarium solani and its opportunistic action related to mycotic keratitis in cali, colombia.	corneal ulcera in patients treated at the university hospital cali, colombia have been attributed to the fungus fusarium solani, which was isolated from patients' eyes by deep scraping. the fungus, which was characterized by culture and morphology, was found to grow well at 37 degrees c in sabouraud and potato dextrose agars and in liquid asparagine medium, in which it produced very few spores; at 40 degrees c, it survived for 3 weeks. different levels of pathogenicity were shown by the fungus w ...	1980	7217337
metabolites of fusarium solani related to dihydrofusarubin.		1980	7251476
[levanase from fusarium solani-68].	extracellular levanase was isolated from the culture of fusarium solani-68 by ammonium sulfate precipitation at 0.8 saturation and purified in part by sephadex g-150 gel chromatography. levanase showed maximum activity at ph = 6.0 and t = 45 degrees c. the levanase reaction (hydrolysis of high molecular levane with a molecular weight of 1--5 min) reached a maximum rate at a polysaccharide concentration of 6.7 mg/ml, michaelis constant being 1.5 x 10(-6) m. levanase remained stable at ph 5.4--7.5 ...	1980	7433428
fusarium solani infection of a hypertensive leg ulcer in a diabetic.		1980	7464854
direct evidence for the presence of beta-hydroxyphenylalanine and beta-hydroxytyrosine in cutinase from fusarium solani pisi.		1979	507809
degradation of lignocellulosic material and humus formation by fungi.	cellulase activity and degradation of cellulose and lignin in wheat straw and formation of humus during degradation by aspergillus sp., chaetomium globosum, fusarium solani, paecilomyces varioti, penicillium chrysogenum and trichoderma viride qm9414 were studied. c. globosum, f. solani, p. varioti and p. chrysogenum produced little or no cellulase when grown on cellulose powder. however, all produced cellulase (filter paper degrading activity) when grown on wheat straw. all the cultures degraded ...	1979	507620
an animal model of fusarium solani endophthalmitis.	infection with fusarium solani was established by injecting spores into the anterior chambers of outbred wistar rats. the disease produced was remarkably similar to that reported in man and progressed to a fulminating endophthalmitis. attenuation of the disease could be accomplished by repeated animal passage of the isolate.	1979	312111
fusarium solani endophthalmitis without primary corneal involvement.	a 45-year-old woman developed a fungal endophthalmitis caused by fusarium solani. no primary corneal lesion existed. therefore, the focus of infection was considered endogenous. although disseminated systemic infections with fusarium species have been described in compromised hosts, including a patient with metastatic panophthalmitis, we believe this is the first report of an endogenous endophthalmitis caused by f. solani in a healthy individual.	1979	315715