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data on partial polyhydroxyalkanoate synthase genes (phac) mined from aaptos aaptos marine sponge-associated bacteria metagenome.we report data associated with the identification of three polyhydroxyalkanoate synthase genes (phac) isolated from the marine bacteria metagenome of aaptos aaptos marine sponge in the waters of bidong island, terengganu, malaysia. our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (pcr)-mediated amplification using degenerate primers targeting class i and ii phac genes, sequencin ...201829188224
u1 sndna chromosomal mapping in ten spittlebug species (cercopidade, auchenorrhyncha, hemiptera).spittlebugs, which belong to the family cercopidae (auchenorrhyncha, hemiptera), form a large group of xylem-feeding insects that are best known for causing damage to plantations and pasture grasses. the holocentric chromosomes of these insects remain poorly studied in regards to the organization of different classes of repetitive dna. to improve chromosomal maps based on repetitive dnas and to better understand the chromosomal organization and evolutionary dynamics of multigene families in spit ...201829185797
ph-dependent singlet o2 oxidation kinetics of guanine and 9-methylguanine: an online mass spectrometry and spectroscopy study combined with theoretical exploration.we report a kinetic and mechanistic study on the title reactions, in which 1o2 was generated by the reaction of h2o2 with cl2 and bubbled into an aqueous solution of guanine and 9-methylguanine (9mg) at different ph values. oxidation kinetics and product branching ratios were measured using online electrospray ionization mass spectrometry coupled with absorption and emission spectrophotometry, and product structures were determined by collision-induced dissociation (cid) tandem mass spectrometry ...201829185758
milk consumption in infants under one year of age and variables associated with non-maternal milk consumption.to verify the type of milk consumed by children under one year of age and identify variables associated with non-maternal milk consumption (formula or cow milk).201829185622
use of score and cerebrospinal fluid lactate dosage in differential diagnosis of bacterial and aseptic meningitis.to evaluate bacterial meningitis score (bms) on its own and in association with cerebrospinal fluid (csf) lactate dosage in order to distinguish bacterial from aseptic meningitis.201829185620
in time: publons seeks to attract reviewers and improve peer review. 201829185619
poor school performance: importance of child self-report. 201829185618
simple protocol for population (sanger) sequencing for zika virus genomic regions.a number of zika virus (zikv) sequences were obtained using next-generation sequencing (ngs), a methodology widely applied in genetic diversity studies and virome discovery. however sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences.201829185594
a resistance exercise program improves functional capacity of patients with psoriatic arthritis: a randomized controlled trial.the objective of this study is to assess the effectiveness of resistance training in patients with psoriatic arthritis (psa). the study is a randomized controlled trial with 41 patients aged between 18 and 65 years with diagnosis of psoriatic arthritis (psa). the patients were randomized into the following: intervention group (ig) and control group (cg). the ig underwent resistance exercise twice a week, for 12 weeks. the cg remained with the conventional drug therapy. the outcome measurements w ...201829185133
assessment of the anti-snakebite properties of extracts of aniba fragrans ducke (lauraceae) used in folk medicine as complementary treatment in cases of envenomation by bothrops atrox.extracts of leaves and bark of aniba fragrans are used as tea (decoction) to treat snakebites in communities in the brazilian amazon. the aqueous extract of the leaves of a. fragrans has been proven to be effective against bothrops venom, but only when pre-incubated with the venom. this study sought to assess the potential of different types of extract of this species to inhibit the biological activities of bothrops atrox venom (bav) when used the same way as in folk medicine. the main classes o ...201829183746
isolation and structure elucidation of lipopeptide antibiotic taromycin b from the activated taromycin biosynthetic gene cluster.in the ongoing effort to unlock the chemical potential of marine bacteria, genetic engineering of biosynthetic gene clusters (bgcs) is increasingly used to awake or improve expression of biosynthetic genes that may lead to discovery of novel bioactive natural products. previously, we reported the successful capture, engineering and heterologous expression of an orphan bgc from the marine actinomycete saccharomonospora sp. cnq-490, which resulted in the isolation of the novel lipopeptide antibiot ...201829184121
halistanol sulfates i and j, new sirt1-3 inhibitory steroid sulfates from a marine sponge of the genus halichondria.two new analogs of halistanol sulfate (1) were isolated from a marine sponge halichondria sp. collected at hachijo-jima island. structures of these new halistanol sulfates i (2) and j (3) were elucidated by spectral analyses. compounds 1-3 showed inhibitory activity against sirt 1-3 with ic50 ranges of 45.9-67.9, 18.9-21.1 and 21.8-37.5 μm, respectively. x-ray crystallography of the halistanol sulfate (1) and sirt3 complex clearly indicates that 1 binds to the exosite of sirt3 that we have disco ...201829184120
electrochemical immunosensors - a powerful tool for analytical applications.immunosensors are biosensors based on interactions between an antibody and antigen on a transducer surface. either antibody or antigen can be the species immobilized on the transducer to detect antigen or antibody, respectively. because of the strong binding forces between these biomolecules, immunosensors present high selectivity and very high sensitivity, making them very attractive for many applications in different science fields. electrochemical immunosensors explore measurements of an elec ...201829182930
mechanical unfolding of macromolecules coupled to bond dissociation.single-molecule force spectroscopy has become a powerful tool to investigate molecular mechanisms in biophysics and materials science. in particular, the new field of polymer mechanochemistry has emerged to study how tension may induce chemical reactions in a macromolecule. a rich example is the mechanical unfolding of the metalloprotein rubredoxin coupled to dissociation of iron-sulfur bonds that has recently been studied in detail by atomic force microscopy. here, we present a simple molecular ...201829182873
two new species of staphylocystoides yamaguti, 1959 (cyclophyllidea: hymenolepididae) from the masked shrew sorex cinereus in north america.two new species of staphylocystoides are described from masked shrews sorex cinereus: staphylocystis oligospinosus n. sp. was collected from the vicinity of missoula, montana, united states and staphylocystoides parasphenomorphus n. sp. was collected from the vicinity of whitehorse, yukon territory, canada. morphological differentiation from known species is provided. both species are morphologically closest to staphylocystoides sphenomorphus and to each other. among other characters, staphylocy ...201829182486
experimental zika virus infection of neotropical primates.the establishment of a sylvatic reservoir of zika virus (zikv) in the americas is dependent on the susceptibility of primates of sufficient population density, the duration and magnitude of viremia, and their exposure to the human mosquito-borne transmission cycle. to assess the susceptibility of squirrel (saimiri sp.) and owl monkeys (aotus sp.) to infection, we inoculated four animals of each species with zikv from the current epidemic. viremia in the absence of detectible disease was observed ...201829182145
laser photobiomodulation in pressure ulcer healing of human diabetic patients: gene expression analysis of inflammatory biochemical markers.pressure ulcers (pu) are wounds located mainly on bone surfaces where the tissue under pressure suffers ischemia leading to cellular lesion and necrosis , its causes and the healing process depend on several factors. the aim of this study was evaluating the gene expression of inflammatory/reparative factors: il6, tnf, vegf, and tgf, which take part in the tissue healing process under effects of low-level laser therapy (lllt). in order to perform lesion area analysis, pus were photographed and co ...201829181642
determination of recombinant interferon-α2 in e. coli periplasmic extracts by reversed-phase high-performance liquid chromatography.reversed-phase high-performance liquid chromatography (rp-hplc) has been used to analyze interferon α-2 (ifn-α2) as a pure protein or as a pharmaceutical preparation: a method for analyzing periplasmic ifn-α2 directly in osmotic shock extract has, however, never been reported. this work describes an rp-hplc methodology for the qualitative and quantitative analysis of human ifn-α2a and ifn-α2b directly in bacterial periplasmic extracts or in purified preparations. the analytical method has been s ...201829179059
custom morse taper zirconia abutments: influence on marginal fit and torque loss before and after thermomechanical cycling.the use of zirconia abutments has increased because of aesthetics, but sometimes customization is necessary and its effect is unclear. this study evaluated the marginal fit and torque loss of customized and non-customized aesthetic zirconia abutments associated with morse taper implants before and after thermomechanical cycling. twenty-four implant/abutment/crown sets were divided into three groups (n = 8): zr - non-customized zirconia abutments, zrc - customized zirconia abutments, and ti - tit ...201829179039
unique properties of arginase purified from camel liver cytosol.arginase (arg) is an enzyme involved in urea cycle, where it catalyzes the hydrolysis of l-arginine into l-ornithine and urea. since there is no information about the isolation and purification of arg from camel liver, this investigation was designed to purify and characterize arg from camel liver and compare its molecular and kinetic properties with that reported from other species. camel liver arginase (cl-arg) was purified to homogeneity using heat denaturation followed by ammonium sulphate p ...201829180053
microscopic characterization of orchid mycorrhizal fungi: scleroderma as a putative novel orchid mycorrhizal fungus of vanilla in different crop systems.vanilla is an orchid of economic importance widely cultivated in tropical regions and native to mexico. we sampled three species of vanilla (v. planifolia, v. pompona, and v. insignis) in different crop systems. we studied the effect of crop system on the abundance, type of fungi, and quality of pelotons found in the roots using light and electron microscopy and direct sequencing of mycorrhizal structures. fungi were identified directly from pelotons obtained from terrestrial roots of vanilla pl ...201829177968
a randomized, double-blind, placebo-controlled, proof-of-concept trial of creatine monohydrate as adjunctive treatment for bipolar depression.depressive episodes are a major cause of morbidity and dysfunction in individuals suffering from bipolar disorder. currently available treatments for this condition have limited efficacy and new therapeutic options are needed. extensive research in the pathophysiology of bipolar disorder points to the existence of mitochondrial and bioenergetic dysfunction. we hypothesized that creatine monohydrate, a nutraceutical that works as a mitochondrial modulator, would be effective as an adjunctive ther ...201829177955
impacts of water quality on the corrosion of cast iron pipes for water distribution and proposed source water switch strategy.switch of source water may induce "red water" episodes. this study investigated the impacts of water quality on iron release, dissolved oxygen consumption (δdo), corrosion scale evolution and bacterial community succession in cast iron pipes used for drinking water distribution at pilot scale, and proposed a source water switch strategy accordingly. three sets of old cast iron pipe section (named bp, sp and gp) were excavated on site and assembled in a test base, which had historically transport ...201829179122
functional analysis of the dehydratase domains of a pufa synthase from thraustochytrium in escherichia coli.thraustochytrium sp. 26185, a unicellular marine protist, synthesizes docosahexaenoic acid, an omega-3 very long chain polyunsaturated fatty acid (vlc-pufas), by a polyunsaturated fatty acid (pufa) synthase comprising three large subunits with multiple catalytic dehydratase (dh) domains critical for introducing double bonds at the specific position of fatty acids. to investigate functions of these dh domains, one dh domain from subunit-a and two dh domains from subunit-c of the pufa synthase wer ...201829177940
high-throughput flow cytometry screening of multidrug efflux systems.the resistance nodulation cell division (rnd) family of proteins are inner membrane transporters that associate with periplasmic adaptor proteins and outer membrane porins to affect substrate transport from the cytosol and periplasm in gram-negative bacteria. various structurally diverse compounds are substrates of rnd transporters. along with their notable role in antibiotic resistance, these transporters are essential for niche colonization, quorum sensing, and virulence as well as for the rem ...201829177837
production of monascus pigments as extracellular crystals by cell suspension culture.it is generally accepted that monascus pigments are predominantly cell-bound, including both intracellular and surface-bound pigments. this long-term misconception was corrected in the present work. production of extracellular crystal pigments by submerged culture of monascus sp. was confirmed by microscopic observation and collection of monascus pigments from extracellular broth by direct membrane filtration. following up the new fact, the bioactivity of mycelia as whole-cell biocatalyst for bi ...201829177624
adaptable piezoelectric hemispherical composite strips using a scalable groove technique for a self-powered muscle monitoring system.contrary to traditional planar flexible piezoelectric nanogenerators (pngs), highly adaptable hemispherical shape-flexible piezoelectric composite strip (hs-fpcs) based pngs are required to harness/measure non-linear surface motions. therefore, a feasible, cost-effective and less-time consuming groove technique was developed to fabricate adaptable hs-fpcss with multiple lengths. a single hs-cspng generates 130 v/0.8 μa and can also work as a self-powered muscle monitoring system (sp-mms) to meas ...201829177339
retrograded starch/pectin coated gellan gum-microparticles for oral administration of insulin: a technological platform for protection against enzymatic degradation and improvement of intestinal permeability.gellan gum microparticles coated with colon-specific films based on retrograded starch and pectin was developed for enhancing the oral release of insulin (ins). the system developed promoted an impressive protection of ins (80%) after 120 min of incubation with trypsin and alpha-chymotrypsin, while only 3% of free ins remained intact after the same time, possibility due to the calcium chelating activity of the polymers in inhibiting the proteolytic activity. in vitro ins release in media simulat ...201829175551
large-scale evaluation of a rapid diagnostic test for diarrhea caused by enterotoxigenic escherichia coli targeting the heat-labile toxin.we standardized an immunochromatographic test (ic) for heat-labile toxin i (lt-i) detection using lt-i antibodies and a specific platform containing the apparatus for application, assembly and cutting. ic detected as little as 62.5ng/ml of purified lt-i toxin and presented 91% sensitivity, 99.5% specificity and 96.0% accuracy, thereby proving to be an excellent point-of-care test for the diagnosis of enterotoxigenic e. coli infection in low-income countries.201829175533
inoculation of soil with an isoproturon degrading microbial community reduced the pool of "real non-extractable" isoproturon residues.during pesticides degradation, biogenic non-extractable residues ("apparent ner") may not share the same environmental fate and risks with the "real ner" that are bound to soil matrix. it is not clear how microbial community (mc) inoculation for pesticides degradation would influence the ner composition. to investigate degradation efficiency of pesticides isoproturon (ipu) and ner composition following mc inoculation, clay particles harboring mc that contains the ipu degrading strain, sphingomon ...201829175344
ankyrin repeats as a dimerization module.legionella pneumophila is a pathogen, causing severe pneumonia in humans called legionnaires' disease. ankc (lega12) is a poorly characterized 495-residue effector protein conserved in multiple legionella species. here, we report the crystal structure of a c-terminally truncated ankc (2-384) at 3.2 å resolution. the structure shows seven ankyrin repeats (ars) with unique structural features. ankc forms a dimer along the outer surface of loops between ars. the dimer exists both in the crystal for ...201829175332
brain signature of mild stages of cognitive and behavioral impairment in amyotrophic lateral sclerosis.we aimed to assess the brain signature of cognitive and behavioral impairment in c9orf72-negative non-demented als patients. the study included 50 amyotrophic lateral sclerosis (als) patients (out of 75 initially recruited) and 38 healthy controls. high-resolution t1-weighted and spin-echo diffusion tensor images were acquired in a 3t mri scanner. the multi atlas-based analysis protocol and the freesurfer tool were employed for gray matter assessment, and fiber tractography for white matter eval ...201829175194
genome-wide association study of stem rust resistance in a world collection of cultivated barley.qtl conferring a 14-40% reduction in adult plant stem rust severity to multiple races of pgt were found on chromosome 5h and will be useful in barley breeding. stem rust, caused by puccinia graminis f. sp. tritici (pgt) is an important disease of barley. the resistance gene rpg1 has protected the crop against stem rust losses for over 70 years in north america, but is not effective against the african pgt race ttksk (and its variants) nor the domestic race qccjb. to identify resistance to these ...201829177535
a self-powered biosensing device with an integrated hybrid biofuel cell for intermittent monitoring of analytes.in this work, we propose an integrated self-powered sensing system, driven by a hybrid biofuel cell (hbfc) with carbon paper discs coated with multiwalled carbon nanotubes. the sensing system has a biocathode made from laccase or bilirubin oxidase, and the anode is made from a zinc plate. the system includes a dedicated custom-built electronic control unit for the detection of oxygen and catechol analytes, which are central to medical and environmental applications. both the hbfc and sensors, op ...201829174971
rpob gene sequencing highlights the prevalence of an e. miricola cluster over other elizabethkingia species among uk cystic fibrosis patients.difficulties in distinguishing species of the elizabethkingia genus by maldi-tof prompted use of rpob sequencing to investigate species distribution among 44 isolates from cystic fibrosis (cf) patients. forty-three isolates from 38 patients formed a cluster comprising e. miricola and proposed novel species e. bruuniana sp. nov., the exception clustering with proposed species e. ursingii sp. nov., also part of this wider cluster. all 44 isolates were pcr-positive for urease gene ureg, whereas onl ...201829174734
response: nitroblue tetrazolium (nbt) assay. 201829174918
diversity of bacterial communities in french chicken cuts stored under modified atmosphere packaging.poultry meat, the second most consumed meat in france, is commercialized mainly as portions of chicken cuts with various quality labels, stored under various modified atmosphere packaging (map), with shelf-life ranging from 9 to 17 days. we used 16s rdna pyrosequencing to describe microbiota of chicken legs. ten samples representing a wide diversity of labels and map available on the market were collected from local supermarkets and stored at 4 °c. microbiota were collected, total dna was extrac ...201829173642
virgin olive oil yeasts: a review.this review summarizes current knowledge on virgin olive oil yeasts. newly produced olive oil contains solid particles and micro drops of vegetation water in which yeasts reproduce to become the typical microbiota of olive oil. to date, about seventeen yeast species have been isolated from different types of olive oils and their by-products, of which six species have been identified as new species. certain yeast species contribute greatly to improving the sensorial characteristics of the newly p ...201829173633
the microbiota of eight species of dehydrated edible seaweeds from north west spain.the microbiota of eight species (chondrus crispus, himanthalia elongata, laminaria ochroleuca, palmaria palmata, porphyra umbilicalis, saccharina latissima, ulva lactuca and undaria pinnatifida) of edible seaweeds collected in north west spain, marketed as dehydrated product, was quantitatively determined on nine solid media. representative colonies were selected from solid culture media. the isolated microorganisms were identified by means of morphological characteristics, 16s rdna sequencing a ...201829173631
cellular imaging and bactericidal mechanism of green-synthesized silver nanoparticles against human pathogenic bacteria.in recent years, silver nanoparticles (agnps) have attracted significant attention in medicinal, biomedical, and pharmaceutical research owing to their valuable physicochemical and antibacterial properties. leaf sap extract (lse) from aloe arborescens can be used as an active ingredient for different biological applications, including wound healing. in this study, we have investigated the use of lse from a. arborescens as a reducing, stabilizing and capping agent to produce agnps during the so c ...201829172133
biodegradation of high concentrations of formaldehyde using escherichia coli expressing the formaldehyde dismutase gene of methylobacterium sp. fd1.in the present study, formaldehyde dismutase from methylobacterium sp. fd1 was partially purified and analyzed by nanolc-ms/ms; it was then cloned from the genomic dna of fd1 by pcr. the open reading frame of the formaldehyde dismutase gene of fd1 was estimated to be 1203 bp in length. the molecular weight and pi of formaldehyde dismutase (401 aa), as deduced from the fd1 gene, were calculated at 42,877.32 and 6.56, respectively. nad(h)-binding residues and zinc-binding residues were found in th ...201829173106
in vivo assessment of plant extracts for control of plant diseases: a sesquiterpene ketolactone isolated from curcuma zedoaria suppresses wheat leaf rust.as an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. in this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against rhizoctonia solani, botrytis cinerea, phytophthora infestans, puccinia triticina, and blumeria graminis f. sp. hordei. of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plan ...201829173073
a sterically constrained tricyclic pc3 phosphine: coordination behavior and insertion of chalcogen atoms into p-c bonds.a tricyclic phosphine has been generated that has a rigid molecular backbone with the p atoms exclusively bound to c(sp2 ) atoms as well as a very large tolman angle and buried volume. it is an interesting new ligand in coordination chemistry (au, pd complexes) and shows unusual insertion reactions into its endocyclic p-c bonds facilitated by its inherent molecular strain.201829171723
design, engineering, and characterization of prokaryotic ligand-binding transcriptional activators as biosensors in yeast.in cell factory development, screening procedures, often relying on low-throughput analytical methods, are lagging far behind diversity generation methods. this renders the identification and selection of the best cell factory designs tiresome and costly, conclusively hindering the manufacturing process. in the yeast saccharomyces cerevisiae, implementation of allosterically regulated transcription factors from prokaryotes as metabolite biosensors has proven a valuable strategy to alleviate this ...201829170965
nitric oxide-cgmp-pkg signaling in the bed nucleus of the stria terminalis modulates the cardiovascular responses to stress in male rats.the bed nucleus of the stria terminalis (bnst) constitutes an important component of neural substrates of physiological and behavioral responses to aversive stimuli, and it has been implicated on cardiovascular responses evoked by stress. nevertheless, the local neurochemical mechanisms involved in bnst control of cardiovascular responses during aversive threats are still poorly understood. thus, the aim of the present study was to assess the involvement of activation in the bnst of the neuronal ...201829169825
chemical metabolome assay by high-resolution orbitrap mass spectrometry and assessment of associated antitumoral activity of actinocephalus divaricatus.actinocephalus divaricatus (eriocaulaceae) is an important source of income for rural communities as it is sold as an ornamental plant. to date, no investigation has been conducted concerning the chemical composition and biological studies of the aerial parts of a. divaricatus.201829166547
resequencing of a mutant bearing an iron starvation recovery phenotype defines slr1658 as a new player in the regulatory network of a model cyanobacterium.photosynthetic microorganisms encounter an erratic nutrient environment characterized by periods of iron limitation and sufficiency. surviving in such an environment requires mechanisms for handling these transitions. our study identified a regulatory system involved in the process of recovery from iron limitation in cyanobacteria. we set out to study the role of bacterioferritin co-migratory proteins during transitions in iron bioavailability in the cyanobacterium synechocystis sp. pcc 6803 usi ...201829161470
characterization and mechanism analysis of lincomycin biodegradation with clostridium sp. strain lcm-b isolated from lincomycin mycelial residue (lmr).lincomycin mycelial residue (lmr) is the restricted resource because it contains residual lincomycin, which is producing potential risks to the environment and human health. in this study, lincomycin-degrading strain lcm-b was isolated and identified as clostridium sp. in the lmr. strain lcm-b was able to degrade 62.03% of lincomycin at the initial concentration of 100 mg l-1 after incubation for 10 d, while only 15.61% of lincomycin was removed at the initial concentration of 500 mg l-1. the re ...201829169137
analysis of unbound plasma concentration of oxcarbazepine and the 10-hydroxycarbazepine enantiomers by liquid chromatography with tandem mass spectrometry in healthy volunteers.this study describes the development and validation of a method for the analysis of unbound plasma concentrations of oxcarbazepine (oxc) and of the enantiomers of its active metabolite 10-hydroxycarbazepine (mhd) [s-(+)- and r-(-)-mhd] using liquid chromatography with tandem mass spectrometry (lc-ms/ms). additionally, the free fraction of the drug is described in healthy volunteers (n=12) after the oral administration of 300mg oxc/12h for 5days. plasma aliquots of 200μl were submitted to ultrafi ...201829169112
estrogenic and anti-androgenic effects of the herbicide tebuthiuron in male nile tilapia (oreochromis niloticus).tebuthiuron is a phenylurea herbicide widely used in agriculture that can reach the aquatic environments, possibly posing negative effects to the aquatic biota. phenylurea herbicides, such as diuron, are known to cause estrogenic and anti-androgenic effects in fish, but no such effects were yet reported for tebuthiuron exposure. thus, the aim of this study was to evaluate if tebuthiuron, at environmentally relevant concentrations (100 and 200ng/l) and after 25days of exposure have estrogenic and ...201829169052
a comparative study of pathophysiological alterations in scorpionism induced by tityus serrulatus and tityus bahiensis venoms.scorpionism is a relevant public health problem in several countries in tropical and subtropical regions. in brazil, tityus serrulatus sting can induce acute lung injury in part as a consequence of inflammation. despite the occurrence of other scorpions of tityus genus in brazilian scorpiofauna, the knowledge regarding pulmonary alterations is related to t. serrulatus venom (tsv). here we studied, comparatively, the pathophysiological changes in the rat airways envenomed by tsv or t. bahiensis v ...201829170053
comparative proteomic analysis of lysine acetylation in trypanosomes.protein acetylation is a post-translational modification regulating diverse cellular processes. by using proteomic approaches, we identified n-terminal and ε-lysine acetylated proteins in trypanosoma cruzi and trypanosoma brucei, which are protozoan parasites that cause significant human and animal diseases. we detected 288 lysine acetylation sites in 210 proteins of procyclic form, an insect stage of t. brucei, and 380 acetylation sites in 285 proteins in the form of the parasite that replicate ...201829168382
capillaria appendigera n. sp. (nematoda: capillariidae) from the goldbanded jobfish pristipomoides multidens (day) (lutjanidae) and new records of other intestinal capillariids from marine perciform fishes off australia.based on light and scanning electron microscopical studies, a new nematode parasite, capillaria appendigera n. sp. (capillariidae), is described from the intestine of the goldbanded jobfish pristipomoides multidens (day) (perciformes, lutjanidae) from the arafura sea west, off the northern coast of australia. the new species, belonging to the subgenus procapillaria moravec, 1987, differs from other congeneric species from fishes mainly in the length (0.92-1.13 mm), shape and structure of the spi ...201829168151
revision of podocotyloides yamaguti, 1934 (digenea: opecoelidae), resurrection of pedunculacetabulum yamaguti, 1934 and the naming of a cryptic opecoelid species.despite morphological and ecological inconsistencies among species, all plagioporine opecoelids with a pedunculate ventral sucker are currently considered to belong in the genus podocotyloides yamaguti, 1934. we revise the genus based on combined morphological and phylogenetic analyses of novel material collected from haemulid fishes in queensland waters that we interpret to represent species congeneric with the type-species, pod. petalophallus yamaguti, 1934, also known from a haemulid, off jap ...201829168149
hypoxia increases thyroid cancer stem cell-enriched side population.hypoxic stress is a feature of rapidly growing thyroid tumours. cancer progression is thought to be driven by a small population of tumour cells possessing stem cell properties. hypoxia-inducible factors (hifs) are important mediators of hypoxia. both hif-1alpha and hif-2alpha have been reported to be expressed in thyroid cancers. there is growing evidence that the hif pathway plays a significant role in the maintenance of thyroid cancer stem cells (csc).201829167950
identifying dna mismatches at single-nucleotide resolution by probing individual surface potentials of dna-capped nanoparticles.here, we demonstrate a powerful method to discriminate dna mismatches at single-nucleotide resolution from 0 to 5 mismatches (χ0 to χ5) using kelvin probe force microscopy (kpfm). using our previously developed method, we quantified the surface potentials (sps) of individual dna-capped nanoparticles (dcnps, ∼100 nm). on each dcnp, dna hybridization occurs between ∼2200 immobilized probe dna (pdna) and target dna with mismatches (tdna, ∼80 nm). thus, each dcnp used in the bioassay (each pdna-tdna ...201829167849
palladium-catalyzed intermolecular acylation of aryl diazoesters with ortho-bromobenzaldehydes.in this work, we describe a palladium-catalyzed intermolecular o acylation of α-diazoesters with ortho-bromobenzaldehydes. the c(sp2 )-h bond activation of the aldehyde is enabled by migratory insertion of a palladium carbene intermediate. the diazoesters act as modular three-atom units to build up key seven-membered palladacycles, which are transformed into a variety of isocoumarin derivatives upon reductive elimination. mechanistic experiments and dft calculations provide insight into the reac ...201829165904
detection and sizing of ti-containing particles in recreational waters using single particle icp-ms.single particle inductively coupled plasma mass spectrometry (spicp-ms) was used to detect ti-containing particles in heavily-used bathing areas of a river (salt river) and five swimming pools. ti-containing particle concentrations in swimming pools ranged from 2.8 × 103 to 4.4 × 103 particles/ml and were an order of magnitude lower than those detected in the salt river. measurements from the salt river showed an 80% increase in ti-containing particle concentration over baseline concentration du ...201829164274
trypanosoma cruzi tryparedoxin ii interacts with different peroxiredoxins under physiological and oxidative stress conditions.trypanosoma cruzi, the etiologic agent of chagas disease, has to cope with reactive oxygen and nitrogen species during its life cycle in order to ensure its survival and infection. the parasite detoxifies these species through a series of pathways centered on trypanothione that depend on glutathione or low molecular mass dithiol proteins such as tryparedoxins. these proteins transfer reducing equivalents to peroxidases, including mitochondrial and cytosolic peroxiredoxins, tcmpx and tccpx, respe ...201829162347
usual interstitial pneumonia: typical, possible, and "inconsistent" patterns.idiopathic pulmonary fibrosis is a severe and progressive chronic fibrosing interstitial lung disease, a definitive diagnosis being established by specific combinations of clinical, radiological, and pathological findings. according to current international guidelines, hrct plays a key role in establishing a diagnosis of usual interstitial pneumonia (uip). current guidelines describe three uip patterns based on hrct findings: a typical uip pattern; a pattern designated "possible uip"; and a patt ...201829160385
extensive hydrolysis of raw rice starch by a chimeric α-amylase engineered with α-amylase (amyp) and a starch-binding domain from cryptococcus sp. s-2.recombinant chimeric α-amylase (amyp-cr) was constructed by a catalytic core of α-amylase (amyp) from a marine metagenomic library and a starch-binding domain (sbdcr) of α-amylase from cryptococcus sp. s-2. the molecular fusion did not alter optimum ph, optimum temperature, hydrolysis products, and an ability of preferential and rapid degradation towards raw rice starch, but catalytic efficiency and thermostability were remarkably improved compared with those of the wild-type amyp. amyp-cr achie ...201829159586
rheinheimera marina sp. nov., isolated from a deep-sea seamount.a bacterial strain designated tp462t, isolated from a seamount near the yap trench in the tropical western pacific, was characterized using a polyphasic taxonomic approach. strain tp462t was found to be gram-stain-negative, aerobic, rod-shaped and motile by means of a single polar flagellum. growth occurred at 4-37 °c (optimum, 25-30 °c) and with 0-4.0 % nacl (optimum, 2-3 %). phylogenetic analysis based on 16s rrna gene sequence showed that strain tp462t was related to the genus rheinheimera an ...201829160196
the impact of urban gardens on adequate and healthy food: a systematic review.to examine the impacts on food and nutrition-related outcomes resulting from participation in urban gardens, especially on healthy food practices, healthy food access, and healthy food beliefs, knowledge and attitudes.201829160186
modified mycotoxins: an updated review on their formation, detection, occurrence, and toxic effects.modified mycotoxins are metabolites that normally remain undetected during the testing for parent mycotoxin. these modified forms of mycotoxins can be produced by fungi or generated as part of the defense mechanism of the infected plant. in some cases, they are formed during food processing. the various processing steps greatly affect mycotoxin levels present in the final product (free and modified), although the results are still controversial regarding the increase or reduction of these levels ...201829158197
experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding.the objective of the present study was to investigate the long-term effect of 0.01% acrolein (acr) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive.201829158188
selective observation of charge storing ions in supercapacitor electrode materials.nuclear magnetic resonance (nmr) spectroscopy has emerged as a useful technique for probing the structure and dynamics of the electrode-electrolyte interface in supercapacitors, as ions inside the pores of the carbon electrodes can be studied separately from bulk electrolyte. however, in some cases spectral resolution can limit the information that can be obtained. in this study we address this issue by showing how cross polarisation (cp) nmr experiments can be used to selectively observe the in ...201829157868
structure and function of a novel antioxidant peptide from the skin of tropical frogs.the amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or uv radiation. based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. by a peptidomic approach, a novel antioxidant peptide (aop) with in vitro free radical scavenging ability was isolated from physalaemus nattereri. the aop, named antioxidin-i, has a molecular weight [m+h]+ = 1543.69da and a twyf ...201829162516
successful cochlear implantation in langerhans cell histiocytosis: a rare case.an 8-year-old girl presented with a 1-year history of bilateral progressive hearing loss and vertigo for 6 months. high-resolution computed tomography of the temporal bones demonstrated multiple lytic lesions. histopathology examination confirmed a diagnosis of langerhans cell histiocytosis (lch). she underwent chemotherapy for 12 months. following treatment, she was in remission. however, the bilateral profound sensorineural hearing loss persisted. she underwent right cochlear implantation with ...201829157165
effect of dietary supplementation with spirulina platensis on atrazine-induced oxidative stress- mediated hepatic damage and inflammation in the common carp (cyprinus carpio l.).the present study evaluated the potential modulatory effect(s) of dietary supplementation with spirulina platensis (sp) on atrazine (atz)-induced oxidative stress and inflammation in common carp (cyprinus carpio l.). common carp was exposed to atz (428μg/l) and sp (1%), either alone or in combination, for 40 days. subsequently, the treatment groups were evaluated for atz-induced oxidative stress-mediated hepatic damage and the potential antioxidant effect(s) of sp supplementation. the results in ...201829156305
phospholipase a1-based cross-reactivity among venoms of clinically relevant hymenoptera from neotropical and temperate regions.molecular cross-reactivity caused by allergen homology or cross-reactive carbohydrate determinants (ccds) is a major challenge for diagnosis and immunotherapy of insect venom allergy. venom phospholipases a1 (pla1s) are classical, mostly non-glycosylated wasp and ant allergens that provide diagnostic benefit for differentiation of genuine sensitizations from cross-reactivity. as ccd-free molecules, venom pla1s are not causative for ccd-based cross-reactivity. little is known however about the pr ...201829156294
a systematic review of interventions and performance measures for antifungal stewardship programmes.antifungal resistance is a significant and emerging threat. stewardship programmes (sps) have been proposed as an opportunity to optimize antifungal use. while examples of antifungal sp implementation have been recently described, there is yet to be an overview of interventions and their impacts on performance measures.201829155990
novel spiroplasma spp. cultured from brains and lymph nodes from ruminants affected with transmissible spongiform encephalopathy.spiroplasma spp., tiny filterable wall-less bacteria, are consistently associated with the transmissible spongiform encephalopathies (tse). spiral forms have been transiently isolated from tse-affected brain tissues in sp4 growth media designed for isolation of spiroplasma spp., but the isolate could not be propagated in sp4 media. a bacterium must grow in vitro in cell-free cultures to allow full characterization of a suspect pathogen. here, a novel spiroplasma sp. was isolated from scrapie- an ...201829155968
can chemotherapy-related acute care visits be accurately identified in administrative data?there is increasing interest in using administrative data to examine treatment-related complications that lead to emergency department (ed) visits or hospitalizations (h). the purpose of this study was to evaluate the reliability of billing codes for identifying chemotherapy-related acute care visits (crvs) among women with early-stage breast cancer.201829155611
localization of surfactant protein-d in the rheumatoid synovial membrane.surfactant protein-d (sp-d) is a collectin, which plays an important role in airway protection and inflammation. the molecule has both pro- and anti-inflammatory capacities depending on its molecular size. its involvement in joint diseases is largely unknown and the aim of this investigation was to study sp-d occurrence and distribution in the synovial membrane of patients with long-standing rheumatoid arthritis (ra) and osteoarthritis (oa). six ra patients and six oa patients, who underwent tot ...201829155458
cell culture models of fatty acid overload: problems and solutions.high plasma levels of fatty acids occur in a variety of metabolic diseases. cellular effects of fatty acid overload resulting in negative cellular responses (lipotoxicity) are often studied in vitro, in an attempt to understand mechanisms involved in these diseases. fatty acids are poorly soluble, and thus usually studied when complexed to albumins such as bovine serum albumin (bsa). the conjugation of fatty acids to albumin requires care pertaining to preparation of the solutions, effective fre ...201829155055
cancer stem cell-like population is preferentially suppressed by egfr-tkis in egfr-mutated pc-9 tumor models.although the epidermal growth factor receptor (egfr) and wnt/β-catenin signaling systems synergistically regulate many essential developmental and regenerative processes in lung cancer, the mechanisms of their crosstalk remain poorly defined. our study aimed to investigate an interaction between egfr and the β-catenin signal.201829154817
biosynthesis of microcystin hepatotoxins in the cyanobacterial genus fischerella.microcystins (mcs) are serine/threonine phosphatase inhibitors synthesized by several members of the phylum cyanobacteria. mining the draft genome sequence of the nostocalean mc-producing fischerella sp. strain cena161 led to the identification of three contigs containing mcy genes. subsequent pcr and sanger sequencing allowed the assembling of its complete biosynthetic mcy gene cluster with 55,016 bases in length. the cluster encoding ten genes (mcya-j) with a central bidirectional promoter was ...201829154789
functionalization of paramagnetic nanoparticles for protein immobilization and purification.a paramagnetic nanocomposite coated with chitosan and n-(5-amino-1-carboxy-pentyl) iminodiacetic acid (nta) that is suitable for protein immobilization applications has been prepared and characterized. the nanoparticle core was synthesized by controlled aggregation of fe3o4 under alkaline conditions, and transmission electron microscopy revealed a size distribution of 10-50 nm. the nanoparticle core was coated with chitosan and derivatized with glutaraldehyde and nta, as confirmed by fourier tra ...201829154787
efficient production of tri-acetylated mono-acylated mannosylerythritol lipids by sporisorium sp. aff. sorghi sam20.the aim of this study was to isolate a novel yeast strain, evaluate biosurfactant production by the strain and characterize the major product.201829154479
effects of temperature and hrt on performance of a novel insulated anaerobic filter (iaf) system incorporated with the waste heat input for building wastewater treatment.effects of temperature and hydraulic retention time (hrt) on the performance of the novel insulated anaerobic filter system (iaf) incorporated with the waste heat input in treating building wastewater were investigated. in this study, an electric heater was used to simulate the waste heat input from air conditioner to the iaf system. the wastewater was collected from an office building in phitsanulok province, thailand. the hrts of iaf system were varied to 9, 18 and 27 h, whereas the water temp ...201829154153
mouthpart deformities in chironomidae (diptera) as bioindicators of heavy metals pollution in shiroro lake, niger state, nigeria.in this study, mouthpart deformities in chironomid larvae (diptera) were investigated in relation to sediment contamination in the shiroro lake in nigeria. metals and chironomids were sampled monthly at three stations (a-c) between august 2013 and january 2014. across the stations, zinc ranged (3.9-75mg/g), manganese (1.29-1.65mg/g), lead (0.00-0.10mg/g), iron (101-168mg/g) and copper (0.13-0.17mg/g). the metal ions did not differ significantly (p > 0.05) between the sampling stations. however, ...201829154140
effect of laser activated bleaching on the chemical stability and morphology of intracoronal dentin.to evaluate the effect of the bleaching with 35% hydrogen peroxide either activated or not by a 970nm diode laser on the chemical stability and dentin surface morphology of intracoronary dentin.201829153956
induction of immune-related gene expression by seminal exosomes in the porcine endometrium.seminal plasma (sp) is considered as a vehicle to carry sperm into female reproductive tract, of which functions have not been completely understood. this study aimed to identify the function of seminal exosomes on porcine endometrium. exosomes were isolated from the sperm-rich fraction of boar semen and were confirmed by the expression of exosome marker hsp70 and size distribution using nano-sight tracking analysis. porcine endometrial epithelial cells (eecs) were then treated with seminal exos ...201829155178
polymorphisms in inflammasome genes and risk of asthma in brazilian children.considering its role in inflammation and recently described "alternative" roles in epithelial homeostasis and th1/th2 balance, we hypothesize that inflammasome genetics could contribute to the development of asthma. selected functional polymorphisms in inflammasome genes are evaluated in a cohort of asthmatic children and their families. gain-of-function nlrp1 variants rs11651270, rs12150220 and rs2670660 resulted significantly associated to asthma in trios (tdt) analysis; and rs11651270 and rs2 ...201829154202
translation, cross-cultural adaptation, and measurement properties of the brazilian version of the identification of functional ankle instability (idfai) questionnaire.the aim of this research was to describe the translation and cultural adaptation, and to test the validity and reliability of the brazilian version of the identification of functional ankle instability (idfai) questionnaire.201829149639
three new species of gyrodactylus von nordmann, 1832 described from goodea atripinnis (pisces: goodeidae), an endemic freshwater fish from the central highlands of mexico.goodea atripinnis jordan, 1880 has a broad range of habitats and is the most widespread species of the endemic goodeid fishes, which inhabit the central highlands of mexico. this species is known to be host to a high diversity of helminth parasites from which only three belong to the genus gyrodactylus von nordmann, 1832: g. lamothei mendoza-palmero, sereno-uribe et salgado-maldonado, 2009, g. mexicanus mendoza-palmero, sereno-uribe et salgado-maldonado, 2009, and g. tomahuac rubio-godoy, razo-m ...201829150699
community composition and ultrastructure of a nitrate-dependent anaerobic methane-oxidizing enrichment culture.methane is a very potent greenhouse gas and can be oxidized aerobically or anaerobically through microbe-mediated processes, thus decreasing methane emissions in the atmosphere. using a complementary array of methods, including phylogenetic analysis, physiological experiments, and light and electron microscopy techniques (including electron tomography), we investigated the community composition and ultrastructure of a continuous bioreactor enrichment culture, in which anaerobic oxidation of meth ...201829150508
dynamics of heterotrophic bacterial assemblages within synechococcus cultures.interactions between photoautotrophic and heterotrophic microorganisms are central to the marine microbial ecosystem. lab cultures of one of the dominant marine photoautotrophs, synechococcus, have historically been difficult to render axenic, presumably because these bacteria depend upon other organisms to grow under these conditions. these tight associations between synechococcus and heterotrophic bacteria represent a good relevant system to study interspecies interactions. ten individual syne ...201829150500
simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device.in this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. this paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (crn) and uric acid (ua) in urine samples. the microfluidic paper-based analytical device (μpad) consists of a main channel with three identical arms, each containing a circular testing zone and a cir ...201829149990
detection of coffee adulteration with soybean and corn by capillary electrophoresis-tandem mass spectrometry.the detection of coffee adulteration with soybean and corn by capillary electrophoresis-tandem mass spectrometry was accomplished by evaluating the monosaccharides profile obtained after acid hydrolysis of the samples. the acid hydrolysis, using h2so4 as a catalyst, increases the ionic strength of the sample impairing the electrophoretic separation. therefore, ba(oh)2 was used to both neutralize the medium and reduce the content of sulfate by precipitation of baso4. the best separation of nine d ...201829146342
study of human salivary proline-rich proteins interaction with food tannins.in this work, saturation transfer difference-nmr, isothermal microcalorimetry and molecular dynamics simulations have been used to study the individual interactions between basic, glycosylated and acidic proline-rich proteins (bprps, gprps, aprps) and p-b peptide with some representative food tannins [procyanidin b2, procyanidin b2 3'-o-gallate (b2g) and procyanidin trimer (catechin-4-8-catechin-4-8-catechin)]. results showed that p-b peptide was in general the salivary protein (sp) with higher ...201829146325
endophytism or saprophytism: decoding the lifestyle transition of the generalist fungus phomopsis liquidambari.filamentous ascomycete phomopsis sp. are common inhabitants of natural ecosystems and, as saprophytes, are largely responsible for the destructive decay of litterfall, promoting the carbon and nitrogen cycles. phomopsis liquidambari b3 can establish mutualistic symbiosis with a broad spectrum of crop plants. colonizing dynamics observations and a growth promotion assay of rice and arabidopsis thaliana revealed that the b3 colonization strategy is host-adapted and resulted in different growth pro ...201829146266
screening of tropically derived, multi-trait plant growth- promoting rhizobacteria and evaluation of corn and soybean colonization ability.the present study assessed the plant growth-promoting (pgp) traits and diversity of culturable rhizobacteria associated with guarana (paullinia cupana), a typical tropical plant. ninety-six bacteria were isolated, subjected to biochemical tests, and identified by partial or total 16s rdna sequencing. proteobacteria and firmicutes were the dominant rhizospheric phyla found, and burkholderia and bacillus were the most abundant genera. thirteen strains exhibited the four pgp traits evaluated, and m ...201829146258
klebsiella sp. confers enhanced tolerance to salinity and plant growth promotion in oat seedlings (avena sativa).plant growth and yield is adversely affected by soil salinity. salt tolerant plant growth-promoting rhizobacteria (pgpr) strain ig 3 was isolated from rhizosphere of wheat plants. the isolate ig 3 was able to grow in presence of nacl ranging from 0 to 20% in luria bertani medium. the present study was planned to evaluate the role of inoculation of pgpr strain ig 3 and its efficacy in augmenting salt tolerance in oat (avena sativa) under nacl stress (100mm). the physiological parameter such as sh ...201829146257
proteomic analysis reveals that tomato interaction with plant growth promoting bacteria is highly determined by ethylene perception.feeding an increasing global population as well as reducing environmental impact of crops is the challenge for the sustainable intensification of agriculture. plant-growth-promoting bacteria (pgpb) management could represent a suitable method but elucidation of their action mechanisms is essential for a proper and effective utilization. furthermore, ethylene is involved in growth and response to environmental stimuli but little is known about the implication of ethylene perception in pgpb activi ...201829145071
production of 1,2-propanediol in photoautotrophic synechocystis is linked to glycogen turn-over.we utilized a photoautotrophic organism to synthesize 1,2-propanediol from carbon dioxide and water fueled by light. a synthetic pathway comprising mgsa (methylglyoxal synthase), yqhd (aldehyde reductase), and adh (alcohol dehydrogenase) was inserted into synechocystis sp. pcc6803 to convert dihydroxyacetone phosphate to methylglyoxal, which is subsequently reduced to acetol and then to 1,2-propanediol. 1,2-propanediol could be successfully produced by synechocystis, at an approximate rate of 55 ...201829143978
spatial separation of photosynthesis and ethanol production by cell type-specific metabolic engineering of filamentous cyanobacteria.cyanobacteria, which perform oxygenic photosynthesis, have drawn attention as hosts for the direct production of biofuels and commodity chemicals from co2 and h2o using light energy. although cyanobacteria capable of producing diverse chemicals have been generated by metabolic engineering, anaerobic non-photosynthetic culture conditions are often necessary for their production. in this study, we conducted cell type-specific metabolic engineering of the filamentous cyanobacterium anabaena sp. pcc ...201829143082
monorchis lewisi n. sp. (trematoda: monorchiidae) from the surf bream, acanthopagrus australis (sparidae), in moreton bay, australia.we describe monorchis lewisi n. sp. (monorchiidae) from the surf bream, acanthopagrus australis (günther, 1859) (sparidae), in moreton bay, eastern australia. the new species differs from most existing species of monorchis monticelli, 1893 in its possession of an elongate i-shaped excretory vesicle, and from other congeners in the relative configuration of the gut and suckers. ovipusillus mayu dove & cribb, 1998 is re-reported from gnathanodon speciosus (forsskål, 1775) (carangidae) from moreton ...201829145916
possible role of arthrospira platensis in reversing oxidative stress-mediated liver damage in rats exposed to lead.environmental pollutants, particularly metallic elements, mobilized and released into the environment, eventually accumulate in the food chain and thus pose a serious threat to human and animal health. in the present study, the role of arthrospira (spirulina platensis; sp) as a protector against oxidative stress-mediated liver damage induced by an exposure to lead acetate (la; as a metallic pollutant) was assessed. to achieve this aim, rats were orally administered with 300 mg/kg bw sp for 15 da ...201829145152
comparison of radiation dose and image quality of abdominopelvic ct using iterative (aidr 3d) and conventional reconstructions.the purpose of this study is to compare radiation dose and image quality of abdominopelvic ct studies reconstructed with iterative and conventional techniques.201829140117
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