Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| toward milk speciation through the monitoring of casein proteotypic peptides. | the possibility of detecting extraneous milk in singles species cheese-milk has been explored. a mass spectrometry (ms)-based procedure has been developed to detect 'signature peptides', corresponding to the predefined subset of 'proteotypic peptides', as matchless analytical surrogates of the parent caseins. tryptic digests of skimmed milk samples from four species were analyzed by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms. amongst the candidate signature peptide ... | 2010 | 20486267 |
| short communication: identification and technological characterization of yeast strains isolated from samples of water buffalo mozzarella cheese. | sixty yeast cultures were isolated from samples of water buffalo mozzarella, a popular "pasta filata" cheese, originating on 16 farms located in the provinces of salerno, caserta, and frosinone (italy). strains were identified by means of 5.8s internal transcribed spacer rdna pcr-rflp combined with 26s rrna gene partial sequencing and characterized for their ability to exert biochemical properties of technological interest. the recorded dominance of fermenting yeasts such as the lactose-fermenti ... | 2010 | 20494142 |
| genetic diversity in farm animals--a review. | domestication of livestock species and a long history of migrations, selection and adaptation have created an enormous variety of breeds. conservation of these genetic resources relies on demographic characterization, recording of production environments and effective data management. in addition, molecular genetic studies allow a comparison of genetic diversity within and across breeds and a reconstruction of the history of breeds and ancestral populations. this has been summarized for cattle, ... | 2010 | 20500753 |
| cryopreservation of buffalo (bubalus bubalis) semen in bioxcell extender. | this study was designed to compare commercially available extender bioxcell with tris-citric egg yolk extender for post thaw quality and in vivo fertility of buffalo semen. for comparison of post thaw semen quality: semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with artificial vagina (at 42 degrees c) for three weeks (replicates). qualifying ejaculates having motility >60% from each buffalo bull were divided in two aliquots and diluted (at 37 ... | 2010 | 20570331 |
| ultrastructure of in vitro oocyte maturation in buffalo (bubalus bubalis). | the objective of the present study was to describe ultrastructural changes in the nucleus and cytoplasmic organelles during in vitro maturation (ivm) of buffalo cumulus-oocyte complexes (cocs). the structures were collected by ovum pick-up (opu). some cocs, removed from maturation medium at 0, 6, 12, 18 and 24 h, were processed for transmission electron microscopy. the average number of cocs collected by opu/animal/session was 6.4, and 44% of them were viable. immature oocytes had a peripherally ... | 2010 | 20576206 |
| water buffalo genome science comes of age. | the water buffalo is vital to the lives of small farmers and to the economy of many countries worldwide. not only are they draught animals, but they are also a source of meat, horns, skin and particularly the rich and precious milk that may be converted to creams, butter, yogurt and many cheeses. genome analysis of water buffalo has advanced significantly in recent years. this review focuses on currently available genome resources in water buffalo in terms of cytogenetic characterization, whole ... | 2010 | 20582226 |
| ketosis in buffalo (bubalus bubalis): clinical findings and the associated oxidative stress level. | as little is known about the oxidant/antioxidant status in buffalo with ketosis, the present study was delineated to assess the oxidative stress level associated with clinical ketosis in water buffalo. a total of 91 parturient buffalo at smallholder farms were studied (61 suspected to be ketotic and 30 healthy). clinical and biochemical investigations were carried out for each buffalo. based on clinical findings and the level of beta-hydroxybutyrate (bhb), buffalo were allocated into ketotic (42 ... | 2010 | 20582718 |
| beneficial impact on cardiovascular risk profile of water buffalo meat consumption. | meat is a good source of proteins and irons, yet its consumption has been associated with unfavorable cardiovascular effects. whether this applies to all types of meat is unclear. we thus aimed to appraise the impact of water buffalo meat consumption on cardiovascular risk profile with an observational longitudinal study. | 2010 | 20588291 |
| the role of mc1r gene in buffalo coat color. | melanocortin-1 receptor (mc1r) plays a major role in pigmentation in many species. to investigate if the mc1r gene is associated with coat color in water buffalo, the coding region of mc1r gene of 216 buffalo samples was sequenced, which included 49 black river buffalo (murrah and nili-ravi), 136 swamp buffalo (dehong, diandongnan, dechang, guizhou, and xilin) with white and gray body, and 31 hybrid offspring of river buffalo nili-ravi (or murrah) and swamp buffalo. among the three variation sit ... | 2010 | 20596837 |
| comparative efficacy of immunological, molecular and culture assays for detection of group a rotavirus from faecal samples of buffalo (bubalus bubalis) calves. | group a rotaviruses play an important role in causing gastroenteritis and mortality in buffalo (bubalus bubalis) calves. a number of assays like rna-polyacrylamide gel electrophoresis (rna-page), enzyme-linked immunosorbent assay (elisa), reverse transcription polymerase chain reaction (rt-pcr) and virus isolation have been employed for rotavirus diagnosis. we evaluated the comparative efficacy of different assays for detection of group a rotavirus in buffalo calves. a total of 455 faecal sample ... | 2010 | 20607400 |
| comparative study on responses of cattle and water buffalo (bubalus bubalis) to experimental inoculation of brucella abortus biovar 1 by the intraconjunctival route--a preliminary report. | the preliminary study was conducted to assess the virulence of a strain of brucella abortus (1969d) and to compare the susceptibility of water buffalo and cattle calves to infection by the intraconjunctival route. seven of each cattle and water buffalo (bubalus bubalis) calves aged 3-6 months were inoculated intraconjunctivally with counts ranging from 1.5 × 10(7) to 1.7 × 10(10) colony forming units of b. abortus. animals were monitored over an 8-week period for clinical manifestations and sero ... | 2010 | 20614238 |
| the influence of cumulus cells during in vitro fertilization of buffalo (bubalus bubalis) denuded oocytes that have undergone vitrification. | the aim of this work was to evaluate whether providing a support of cumulus cells during ivf of buffalo denuded oocytes submitted to vitrification-warming enhances their fertilizing ability. in vitro matured denuded oocytes were vitrified by cryotop in 20% eg + 20% of dmso and 0.5 m sucrose and warmed into decreasing concentrations of sucrose (1.25 m-0.3m). oocytes that survived vitrification were fertilized: 1) in the absence of a somatic support (dos); 2) in the presence of bovine cumulus cell ... | 2010 | 20615538 |
| study on the inter-subspecies nuclear transfer of river buffalo somatic cell nuclei into swamp buffalo oocyte cytoplasm. | the objective of this study was to explore the feasibility of inter-subspecies somatic cell nuclear transfer (scnt) of river buffalo (50 chromosomes) somatic cell nuclei into swamp buffalo (48 chromosomes) oocyte cytoplasm. the enucleated swamp buffalo oocytes were fused with four different types of river buffalo cells: freshly thawed ear fibroblasts, serum-starved ear fibroblasts, cumulus cells and ear fibroblasts from a cloned buffalo calf. as a result, the developmental competence of embryos ... | 2010 | 20621244 |
| impact of livestock hygiene education programs on mastitis in smallholder water buffalo (bubalus bubalis) in chitwan, nepal. | a project implemented from 2003 to 2005 trained women in chitwan district, nepal, in hygienic dairy production using a process of social mobilization. the aim of this research was to assess if the prevalence of mastitis in water buffalo in the households of women who were trained was lower one year after training than in untrained households, if the training influenced knowledge and practices for the prevention or control of mastitis, and if these practices and knowledge were associated with a l ... | 2010 | 20655119 |
| prevalence and molecular diagnosis of trypanosoma evansi in nili-ravi buffalo (bubalus bubalis) in different districts of punjab (pakistan). | the prevalence of trypanosoma evansi was investigated in 1,250 nili-ravi buffaloes of mixed age and sex by polymerase chain reaction (pcr) for the first time in pakistan. dna of the trypanosomes was isolated with trireagent®. the assay was employed using primers esag 6/7, specific for a 237-bp fragment from t. evansi genomic dna. the samples were screened for the presence of t. evansi also by stained thin smear. forty-four (3.5%) samples were positive by microscopy, while 97 (7.7%) samples were ... | 2010 | 20680446 |
| tissue-specific promoter methylation coincides with cyp19 gene expression in buffalo (bubalus bubalis) placenta of different stages of gestation. | aromatase is the key enzyme for estrogen biosynthesis and is encoded by cyp19 gene. placental cotyledons are the main site of cyp19 gene expression during pregnancy. the present study was aimed to investigate if dna methylation and thus epigenetic mechanisms play a potential role in stage-specific regulation of cyp19 expression in placental cotyledons of pregnant water buffaloes (bubalus bubalis). significantly higher expression of cyp19 gene (p<0.05) in placental cotyledons of early gestation p ... | 2010 | 20691690 |
| sequence analysis and genetic variability of stearoyl coa desaturase (scd) gene in the italian mediterranean river buffalo. | stearoyl-coa desaturase (scd) plays a key metabolic role by changing the saturated fa content of ruminant milk and meat. in this study we characterized for the first time the stearoyl-coa desaturase (scd) gene in river buffalo (bubalus bubalis) and investigated its genetic variability. on a total of 78 buffaloes, 15 snps were detected and 6 of them were preliminarily genotyped. in particular, the g.133a>c snp was found to create a new consensus site for the sp1 binding site, thus generating a ne ... | 2010 | 20691778 |
| purification, characterization and expression kinetics of heat shock protein 70 from bubalus bubalis. | the present work on bubalus bubalis (buffalo) was designed to study heat shock protein 70 (hsp70) induction in lymphocytes, its purification and characterization. hsp70 induction and expression kinetics at different temperatures and time durations were also studied. hsp70 purification was carried out by immunoaffinity chromatography using adenosine di-phosphate (adp-agarose column) and the characterization of the purified protein was done using western blotting by mouse monoclonal anti-hsp70. th ... | 2010 | 19836035 |
| a single nucleotide polymorphism and sequence analysis of csn1s1 gene promoter region in chinese bos grunniens (yak). | the aim of this study was to investigate the polymorphism of the csn1s1 gene promoter region in 4 chinese yak breeds, and compare the yak csn1s1 gene promoter region sequences with other ruminants. a polymerase chain reaction-single strand conformation polymorphism protocol was developed for rapid genotyping of the yak csn1s1 gene. one hundred fifty-eight animals from 4 chinese yak breeds were genotyped at the csn1s1 locus using the protocol developed. a single nucleotide polymorphism of the csn ... | 2010 | 20024785 |
| changes in endogenous estrogens and expression of behaviors associated with estrus during the periovulatory period in heatsynch treated murrah buffaloes (bubalus bubalis). | the objective of this study was (1) to establish the duration of behavioral estrus signs and timing of ovulation in murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations in animals treated with heatsynch protocol. estrus and its behavioral signs were detected at hourly intervals by visual observations per rectal examination of genitalia and bull parading four times in a day for 30 min each. among the behavioral s ... | 2010 | 19997777 |
| seven novel single nucleotide polymorphisms identified within river buffalo (bubalus bubalis) lactoferrin gene. | the present study aimed at identifying single-nucleotide polymorphic (snp) sites in different coding and non-coding regions of lactoferrin gene in indian riverine buffaloes. a total of 102 animals from six different river buffalo breeds were screened at six bubaline lactoferrin gene loci. single-strand conformation polymorphism (sscp) analysis revealed monomorphic patterns at three loci ltfe2, ltfe11, and ltfe14 while a total of eight distinct patterns were observed in the other three loci viz. ... | 2010 | 20012192 |
| zearalenone is bioactivated in the river buffalo (bubalus bubalis): hepatic biotransformation. | zearalenone (zea) as a mycoestrogen is found frequently in human foods and animal feeds. its estrogenic effects depend on its biotransformation fate including both first- and second-phase reactions, which are predominantly governed by hydroxylation and glucuronidation, respectively. in this study, we investigate the hepatic biotransformation of zea in river buffalo. to evaluate the hepatic biotransformation of zea, both subcellular fractions of the liver were prepared. zea was incubated with int ... | 2010 | 20361255 |
| characterization of bison (bison bison) myoglobin. | bison is an alternate meat species gaining increased popularity in north america. although previous investigations reported that bison meat discolors faster than beef, the molecular basis of this observation has not been investigated. therefore, the objective of the present study was to determine the redox stability, thermostability, and primary structure of bison myoglobin (mb), in comparison with beef mb. purified bison and beef myoglobins were analyzed for autoxidation, lipid oxidation-induce ... | 2010 | 20374756 |
| changes in luteal cells distribution, apoptotic rate, lipid peroxidation levels and antioxidant enzyme activities in buffalo (bubalus bubalis) corpus luteum. | buffalo (bubalus bubalis) is known for its weak/silent estrous behaviour, lower conception rate and longer inter-calving interval as compared to cattle. understanding the kinetics and functional properties of luteal cells may be helpful to improve reproductive efficiency in the buffalo. hence the present study was designed to assess the size and distribution of steroidogenic luteal cells along with biochemical properties during different phases of corpus luteum (cl) in the buffalo. the ovaries c ... | 2010 | 20378285 |
| genetic parameters for stayability in murrah buffaloes. | in order to contribute to the breeding programmes of asian water buffalo, the aim of this study was to analyse the influence of genetic effects in the stayability of murrah dairy buffaloes. the stayability trait (st) was defined as the female's ability to stay in the herd for one (st1), two (st2), three (st3), four (st4), five (st5) or six years (st6) after the first calving. the same trait was also considered as continuous and was designated stayability in days up to one (std1), two (std2), thr ... | 2010 | 20380776 |
| histology of hemal nodes of the water buffalo (bos bubalus). | hemal nodes are independent lymphoid organs found in various mammals but are ignored by most immunologists. they seem to play a role in defense against blood-borne infections in some species. the structure of the hemal node has been described in various species but, so far, not in the water buffalo. specimens were obtained from ten clinically healthy male animals (five calves: 2-3 months old; five bulls: 2-8 years old). six hemal nodes were obtained from each animal from the mesenteric and perir ... | 2010 | 20387076 |
| antipyretic and antioxidant activities of the aqueous extract of cornu bubali (water buffalo horn). | cornu bubali (water buffalo horn, wbh) is an animal-derived product which is widely used in traditional chinese medicine (tcm) for dispelling heat, relieving convulsions and cooling blood. the purpose of this study was to investigate the antipyretic activity of wbh aqueous extract and its potential mechanism. two hyperthermia models, yeast-induced (infectious) and skimmed milk-induced (noninfectious) hyperthermia were employed to evaluate the antipyretic effect and the results showed that rectal ... | 2010 | 20387226 |
| combining aboriginal and non-aboriginal knowledge to assess and manage feral water buffalo impacts on perennial freshwater springs of the aboriginal-owned arnhem plateau, australia. | aboriginal land managers have observed that feral asian water buffalo (bubalis bubalis lydekker) are threatening the ecological and cultural integrity of perennial freshwater sources in arnhem land, australia. here we present collaborative research between the aboriginal rangers from warddeken land management limited and western scientists which quantified the ground-level impacts of buffalo on seven perennial freshwater springs of the arnhem plateau. a secondary aim was to build the capacity of ... | 2010 | 20390401 |
| efficacy of brucella abortus vaccine strain rb51 compared to the reference vaccine brucella abortus strain 19 in water buffalo. | approximately 250,000 water buffalo (bubalus bubalis) live in the campania region of southern italy where the breeding of this species is very popular. of these animals, almost 150,000 are concentrated in the caserta province where the prevalence of brucella abortus in this species represents approximately 20% at herd level. the italian brucellosis eradication programme provides a slaughter and vaccination strategy for this province. b. abortus strain rb51 (rb51) has become the official vaccine ... | 2010 | 20391363 |
| effect of source and dose of probiotics and exogenous fibrolytic enzymes (efe) on intake, feed efficiency, and growth of male buffalo (bubalus bubalis) calves. | probiotics of lactobacillus acidophilus, saccharomyces cerevisiae, and aspergillus niger and three commercial exogenous fibrolytic enzymes (efe) were tested in vitro to select best source and optimum dose followed by in vivo studies on male buffalo calves. bacterial (p < 0.001) and protozoal population were increased significantly (p < 0.001) with probiotics and efe. in vitro dry matter digestibility was more (p < 0.001) on l. acidophilus and then on s. cerevisiae. dose required for l. acidophil ... | 2010 | 20401692 |
| genetic and morphometric characterization of a local vietnamese swamp buffalo population. | the water buffalo plays a key role in the socio-economy of south-east asia as it is the main draught power for paddy rice cultivation. while in the indian subcontinent the water buffalo is the riverine type, in south-east asia the majority of buffaloes are of the swamp type. in the poor remote northern province of ha giang in vietnam, improvement of the swamp buffalo breed may be one of the best ways to increase sustainability of farming systems. therefore, analysis of the genetic structure of t ... | 2010 | 20074189 |
| colostrum and serum lysozyme levels in mediterranean buffaloes (bubalus bubalis) and in their newborn calves. | 2010 | 20081181 | |
| effect of timing of development on total cell number and expression profile of hsp-70.1 and glut-1 in buffalo (bubalus bubalis) oocytes and preimplantation embryos produced in vitro. | the present study was designed to compare the expression profile of two developmentally important genes (hsp-70.1 and glut-1) and tcn (total cell number) count in fast (group a) and slow (group b) cleaved buffalo embryos to access their in vitro developmental competence. buffalo cocs (cumulus oocyte complexes) were collected from local abattoir ovaries and subjected to in vitro maturation in: tcm-199 supplemented with 10% fbs (fetal bovine serum), bsa (3 mg/ml), sodium pyruvate (0.25 mm) and 20 ... | 2010 | 20100172 |
| death by attack from a domestic buffalo. | attacks on humans by domestic animals causing fatal injuries are not uncommon in rural areas of india. but injuries due to buffalo gore are rarely observed in villages and are different from other casualties like stab injuries, road fatalities, etc. as the victims of buffalo attack are usually recovered from the fields or forest, the investigating officer could be mislead as to the nature of infliction of fatal injuries to a possible homicide. the injuries caused by the horns of buffaloes are of ... | 2010 | 20129432 |
| sex-preselected buffalo (bubalus bubalis) calves derived from artificial insemination with sexed sperm. | flow cytometry sorting of x- and y-chromosome bearing sperm has been emerging as a promising technology to alter the sex ratio in progenies of mammals in the recent years. the objective of this study was to evaluate the efficiency of ai by using the sexed sperm to produce sex-preselected calves in buffalo species. a total of 43 buffalo cows were inseminated with x-sorted sperm, 30 of which were confirmed pregnant 3 mo following ai. in terms of conception rate, significant difference was observed ... | 2010 | 20137870 |
| morphological changes, dna damage and developmental competence of in vitro matured, vitrified-thawed buffalo (bubalus bubalis) oocytes: a comparative study of two cryoprotectants and two cryodevices. | present study was designed to investigate the impact of two cryodevices; french mini straw (fms) and open pulled straw (ops) using two different cryoprotectants; ethylene glycol (eg) and propylene glycol (pg) on morphological damage, recovery rate, dna damage and developmental competence of in vitro matured vitrified-thawed buffalo oocytes. in vitro matured oocytes were divided into three groups: (a) no cryoprotectant (unfrozen, control), (b) vitrified in fms and (c) in ops using eg/pg. after th ... | 2010 | 20178778 |
| effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo (bubalus bubalis) sperm quality following cryopreservation. | cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. under the present study, the cryoprotective effect of taurine and trehalose on buffalo sperm quality parameters after freeze-thaw process was studied. buffalo semen was cryopreserved in tris-based egg yolk extender along with cryoprotectants like taurine (50 mm) or trehalose (100 mm) and used for the assessment of sperm quality parameters like motility, viability, plasma membrane integrity, t ... | 2010 | 20197223 |
| purification and identification of three novel antioxidant peptides from cornu bubali (water buffalo horn). | cornu bubali (water buffalo horn, wbh) has been used in traditional chinese medicine (tcm) for thousands of years. in the present study, three peptides with antioxidant properties were purified from aqueous extract of cornu bubali (water buffalo horn, wbh) by consecutive chromatographic methods including gel filtration chromatography, ion-exchange chromatography and high performance liquid chromatography. the sequences of the three peptides were identified to be gln-tyr-asp-gln-gly-val (wbh-1, 7 ... | 2010 | 20206218 |
| should the domestic buffalo (bubalus bubalis) be considered in the epidemiology of bovine herpesvirus 1 infection? | only limited information is available on the epidemiology and pathogenesis of bovine herpesvirus 1 (bohv-1) in domestic buffalos. in this study, a virulent bohv-1 field strain isolated from cattle was inoculated into buffaloes to evaluate their susceptibility to the virus and to investigate the establishment of viral latency through clinical, virological and serological investigations. latency was also studied by attempting viral reactivation using pharmacological induction. six of seven male, 5 ... | 2010 | 20206452 |
| an indirect elisa for detection of theileria sergenti antibodies in water buffalo using a recombinant major piroplasm surface protein. | in this study we investigated the prevalence and enzootic potential of theileria spp. in water buffalo in the hubei province in china. an indirect elisa based on a recombinant major piroplasma surface protein was developed. the complete orf of the 33-kda major piroplasma surface protein (p33) was obtained from theileria sergenti genomic dna by pcr, cloned into the pet-28(a) vector and expressed in e. coli as a his-fusion protein. then the recombinant p33 (rp33) was purified and used as the antig ... | 2010 | 20207484 |
| multiple molecular forms of inhibin in buffalo (bubalus bubalis) ovarian follicular fluid. | inhibin is a heterodimeric glycoprotein hormone involved in the regulation of fsh release from the anterior pituitary gland and it has been characterized from various animals. although, multiple molecular forms of inhibin have been reported from different species, however, the molecular nature of inhibin has not been studied in buffaloes. in the present study, attempts were made to identify inhibin in buffalo ovarian follicular fluid. buffalo ovaries were obtained from the local abattoir and fol ... | 2010 | 20211478 |
| divergent development of testosterone secretion in male zebu (bos indicus) and crossbred cattle (bos indicus x bos taurus) and buffaloes (bubalus bubalis) during growth. | delayed pubertal development and low fertility of bos indicus x bos taurus crossbred male cattle and domestic buffaloes is hardly understood hence, a sensitive enzymeimmunoassay (eia) was developed using the second antibody-coating technique and testosterone-3-o-carboxymethyloxime-horseradish peroxidase conjugate as a label for determination of testosterone in blood plasma. the eia was validated by standard criteria. blood samples were collected by venipuncture from growing male cattle (karan fr ... | 2010 | 20213223 |
| y-chromosomal variation confirms independent domestications of swamp and river buffalo. | y-chromosomal variation in the water buffalo was analysed by sequencing of dby, zfy and sry gene segments. a clear separation of the paternal lineages of the river and swamp types parallels the differences between their maternal lineages and nuclear dna. sequence divergence was found to be comparable to the divergence of taurine cattle and zebu, and this divergence predated domestication, confirming that river and swamp buffalo originated from different wild populations. within a sample of 23 th ... | 2010 | 20219068 |
| cysteine proteases of trypanosoma (megatrypanum) theileri: cathepsin l-like gene sequences as targets for phylogenetic analysis, genotyping diagnosis. | although trypanosomatheileri and allied trypanosomes are the most widespread trypanosomes in bovids little is known about proteolytic enzymes in these species. we have characterized genes encoding for cathepsin l-like (catl) cysteine proteases from isolates of cattle, water buffalo and deer that largely diverged from homologues of other trypanosome species. analysis of 78 catl catalytic domain sequences from 22 t. theileri trypanosomes disclosed 6 genotypes tightly clustered together into the t. ... | 2010 | 20230907 |
| loop-mediated isothermal amplification (lamp) assay for detection of theileria sergenti infection targeting the p33 gene. | theileria is a widespread, intraerythrocytic tick-borne protozoan parasite. loop-mediated isothermal amplification (lamp) is a novel nucleic acid amplification method offering rapid, accurate and cost-effective diagnosis of infectious diseases. in this study, the lamp method was developed for detecting theileria sergenti. four primers were designed from six distinct regions of the target gene, a 33-kda major piroplasm surface protein (p33) gene in t. sergenti. the specificity assay showed it was ... | 2010 | 20334978 |
| purification, characterization, structural analysis and protein chemistry of a buffalo heart galectin-1. | a soluble β-galactoside-binding lectin was purified by gel filtration chromatography from bubalus bubalis heart. its metal-independent nature, molecular weight of 14.5 kda, preferential affinity for β-d-lactose, and 87-92% identity with carbohydrate recognition domain of previously reported galectin-1 confirmed its inclusion in galectin-1 subfamily. stokes radii determination using gel filtration under reducing and non-reducing conditions revealed its homo-dimeric nature, further confirming its ... | 2010 | 20354738 |
| microsatellite-based genetic monitoring to detect cryptic demographic bottleneck in indian riverine buffaloes (bubalus bubalis). | the present study was undertaken to evaluate different indian riverine buffalo breeds (bubalus bubalis) for mutation drift equilibrium and occurrence of any recent genetic bottleneck. a total of 330 animals from seven different breeds were analyzed with a set of 24 heterologous microsatellite markers. three different tests revealed significant heterozygosity excess in all the seven buffalo breeds studied when assumed under infinite alleles model of microsatellite evolution, while it was the reve ... | 2010 | 19936960 |
| antimicrobial resistance in gram-positive bacteria from timorese river buffalo (bubalus bubalis) skin microbiota. | the timorese river buffalo (bubalus bubalis) plays a major role in the east timor economy, as it is an important source of animal protein in human nutrition. they are widely spread throughout the country and are in direct contact with the populations. in spite of this proximity, information on their microbiota is scarce. this work aimed at characterizing the skin microbiota of the east timorese river buffalo and its antimicrobial resistance profile. skin swab samples were taken from 46 animals i ... | 2010 | 19898998 |
| glutathione concentration and gamma-glutamyltransferase activity in water buffalo colostrum. | evidence is presented that the buffalo mammary gland contains enzymes that catalyse the synthesis and utilization of glutathione. a significant, inverse correlation (r = 0.79) was detected between colostrum gamma-glutamyltransferase (ggt) and glutathione (gsh), suggesting that the enzyme uses gsh as a substrate for its activity. a similar trend was shown in mammary gland homogenates (r = 0.75). our results show that gsh is secreted into buffalo colostrum and suggest that the enzyme ggt degrades ... | 2010 | 19906130 |
| occurrence of aflatoxin m1 in raw milk of five dairy species in ahvaz, iran. | during november 2007 to december 2008, 311 samples of raw milk from cow, water buffalo, camel, sheep, and goat were collected in the ahvaz (southwest iran). all of the samples were analyzed for presence of aflatoxin m1 (afm1) by competitive elisa technique. afm1 was found in 42.1% of the samples by average concentration of 43.3+/-43.8 ng/kg. the incidence rates of afm1 in raw cow, water buffalo, camel, sheep, and goat milks were, 78.7%, 38.7%, 12.5%, 37.3%, and 27.1%, respectively. the concentra ... | 2010 | 19786054 |
| an inter-subspecies cloned buffalo (bubalus bubalis) obtained by transferring of cryopreserved embryos via somatic cell nuclear transfer. | the aim of this study was to explore the feasibility of cryopreservation of inter-subspecies cloned embryos in buffalo. in our experiment, river buffalo ear fibroblast nucleus was fused into swamp buffalo oocyte cytoplasm. the blastocyst formation rate for nuclear transfer of freshly thawed cells was not different from those of growing cells, confluent or serum-starved cells. a total of 122 cloned blastocysts derived from cryopreserved fibroblasts were cryopreserved and thawed, 37 were survived, ... | 2010 | 19788521 |
| genetic variation and relationships among turkish water buffalo populations. | the genetic variation and relationships among six turkish water buffalo populations, typical of different regions, were assessed using a set of 26 heterologous (bovine) microsatellite markers. between seven and 17 different alleles were identified per microsatellite in a total of 254 alleles. the average number of alleles across all loci in all the analysed populations was found to be 12.57. the expected mean heterozygosity (h(e)) per population ranged between 0.5 and 0.58. significant departure ... | 2010 | 19799598 |
| testicular development and establishment of spermatogenesis in nili-ravi buffalo bulls. | fifteen longitudinally reared nili-ravi buffalo bulls (bubalus bubalis) were slaughtered at 1, 6, 12, 18, and 24 mo of age (n=3 per group) to observe testicular development and to examine qualitatively the establishment of spermatogenesis. with the age held constant, scrotal circumference and testes weight were correlated (0.95; p<0.05). testes weight increased from 3.5+/-0.7 at 1 mo of age to 185+/-30g at 24 mo of age. seminiferous tubules diameter developed in a linear fashion (57microm at 1 m ... | 2010 | 19815266 |
| effect of donor cell types on developmental potential of cattle (bos taurus) and swamp buffalo (bubalus bubalis) cloned embryos. | this study investigated the effect of donor cell types on the developmental potential and quality of cloned swamp buffalo embryos in comparison with cloned cattle embryos. fetal fibroblasts (ffs), ear fibroblasts (efs), granulosa cells (gcs) and cumulus cells (ccs) were used as the donor cells in both buffalo and cattle. the cloned cattle or buffalo embryos were produced by fusion of the individual donor cells with enucleated cattle or buffalo oocytes, respectively. the reconstructed (cloned) em ... | 2010 | 19815984 |
| detection of mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes. | tissue samples were collected at random from cattle (bos taurus) and buffalo (bubalus bubalis) from an abattoir of the district of lahore and were analyzed for the presence of mycobacterium avium subsp. paratuberculosis and mycobacterium bovis through acid-fast staining and polymerase chain reaction (pcr). body condition of animals and diarrhea were recorded. most of the animals were emaciated. diarrhea was noticed in 15.6% of buffaloes and 19.2% of cattle. intestinal pathology was observed in 2 ... | 2010 | 19816784 |
| the molecular phylogeny of uterine serpins and its relationship to evolution of placentation. | uterine serpins (uss), designated as serpina14, are expressed in the endometrium in response to progesterone. all species identified as having uss exhibit epitheliochorial placentation and are in the ruminantia and suidae orders of the laurasiatheria superorder. the objective was to identify us genes in species within and outside laurasiatheria and evaluate whether evolution of the us gene was associated with development of the epitheliochorial placenta. through queries of nucleotide and genomic ... | 2010 | 19825977 |
| improvement in buffalo (bubalus bubalis) spermatozoa functional parameters and fertility in vitro: effect of insulin-like growth factor-i. | the aim of the current study was to assess the effect of insulin-like growth factor-i (igf-i; 100 ng/ml) on buffalo (bubalus bubalis) sperm functional parameters related to in vitro fertilization. the acrosin activity (the mean diameter of halo formation in micrometers) was significantly higher in the igf-i group (14.17 +/- 1.51) compared with that in the control group (9.50+/-0.36) at 2h incubation. the mitochondrial membrane potential (per cent) was significantly higher in the igf-i group comp ... | 2010 | 19828187 |
| temporal changes in endogenous estrogens and expression of behaviors associated with estrus during the periovulaory period in murrah buffaloes (bubalus bubalis). | the objective of this study were (1) to establish the duration of behavioral estrus signs and timing of ovulation in murrah buffaloes (n = 10) and (2) to determine relationship between behavioral estrus signs with change in plasma estrogen concentrations. estrus and its behavioral signs were detected at hourly intervals by visual observations, per recta examination of genitalia and bull parading four times in a day for 30 minutes each. among the behavioral signs of estrus, swollen vulva (80%) wa ... | 2010 | 19544005 |
| risk factors of infestation by psoroptes spp. mites in buffalo (bubalus bubalis) at smallholder farms in the nile delta region, egypt. | the aim of the present study was to explore the prevalence and potential risk factors associated with infestation by psoroptes spp. mites in buffalo at smallholder farms in the nile delta region, egypt. a total of 318 out of 1234 buffalo in 77 small scale herds were examined. the age of buffalo varied from three months to seven years. in all, 53 (16.66%) buffalo were recorded to be infested; 51 (16.35%) with psoroptes spp. mites and two cases (0.31%) with chorioptes spp. mites. multivariate logi ... | 2010 | 19688306 |
| bacterial diversity in the rumen of gayals (bos frontalis), swamp buffaloes (bubalus bubalis) and holstein cow as revealed by cloned 16s rrna gene sequences. | libraries of rumen bacterial 16s rrna gene sequences of gayals (bos frontalis) and swamp buffaloes (bubalus bubalis) were cloned and sequenced in the present work to compare the bacterial diversity with the third published library of holstein cow. sequence similarity of 97% was used as the definition of operational taxonomic unit (otu). the majority of the 470 sequences retrieved fell into the phyla of low g + c subdivision (329 sequences) and cytophaga-flexibacter-bacteroides (cfb, 123 sequence ... | 2010 | 19662514 |
| ion, protein, phospholipid and energy substrate content of oviduct fluid during the oestrous cycle of buffalo (bubalus bubalis). | the aim of this research was to analyse the composition of oviduct fluid (odf) in buffalo cows at different oestrous cycle phases to fulfil the requirements of buffalo embryos in vitro. odf was collected by chronic cannulation from three cows that were synchronized by administering a synthetic prostaglandin. based on hormonal profiles, the pre-ovulatory, ovulatory, post-ovulatory and luteal phases of the oestrous cycle were defined. the volume of odf produced (ml/24 h) was influenced by the oest ... | 2010 | 19761531 |
| characterization of spliced leader genes of trypanosoma ( megatrypanum) theileri: phylogeographical analysis of brazilian isolates from cattle supports spatial clustering of genotypes and parity with ribosomal markers. | trypanosoma (megatrypanum) theileri from cattle and trypanosomes of other artiodactyls form a clade of closely related species in analyses using ribosomal sequences. analysis of polymorphic sequences of a larger number of trypanosomes from broader geographical origins is required to evaluate the clustering of isolates as suggested by previous studies. here, we determined the sequences of the spliced leader (sl) genes of 21 isolates from cattle and 2 from water buffalo from distant regions of bra ... | 2010 | 19765336 |
| transcriptional analysis of buffalo (bubalus bubalis) oocytes during in vitro maturation using bovine cdna microarray. | the need for improving in vitro production of buffalo embryos necessitates a better understanding of the molecular mechanisms regulating early development including oocyte maturation. here, we used bovine cdna microarray platform to investigate mrna abundance of buffalo oocytes before and after in vitro maturation. for this, a total of six pools each contains 50 immature or in vitro matured buffalo oocytes were used for mrna isolation and subsequent cdna synthesis. the bluechip bovine cdna micro ... | 2010 | 19144006 |
| generation and characterization of embryonic stem-like cell lines derived from in vitro fertilization buffalo (bubalus bubalis) embryos. | in the present study, buffalo embryonic stem-like (es-like) cell lines were successfully isolated, cultured and characterized. from a total of 92 normal buffalo embryos obtained by in vitro fertilization, 18 were morulae, 33 were blastocyst and 41 were hatched blastocyst, the inside of morulae or inner cell masses of blastocysts were isolated mechanically and cultured onto mitomocin-c-inactivated buffalo embryonic fibroblasts as feeder layers. alkaline phosphatase (ap) of es-like cells, as well ... | 2010 | 19144015 |
| generation of buffalo (bubalus bubalis) transgenic chimeric and nuclear transfer embryos using embryonic germ-like cells expressing enhanced green fluorescent protein. | the possibility of producing transgenic buffalo embryos by chimera and nuclear transfer (nt) using buffalo embryonic germ (eg)-like cells expressing enhanced green fluorescent protein (egfp) has been explored in this study. buffalo eg-like cells and fibroblasts with two to eight passages were transfected with the lined plasmid (pce-egfp-ires-neo-dndb) using lipofectamine 2000 and selected by culturing in 200 microg/ml g418 for 6-8 days. g418 resistant fibroblasts and eg-like cells were used for ... | 2010 | 19144016 |
| efficiency of scnt buffalo (bubalus bubalis) embryos in different culture medium and analysis of mrna expression of insulin-like growth factors during embryogenesis. | growth factors in culture media are known to affect the embryo production rates in in vitro production cultures. to improve the efficiency of somatic cell nuclear transfer (scnt) derived embryos in indian buffaloes (bubalus bubalis), embryos were cultured in three different culture mediums viz. group-a; tcm-199 + fbs, group-b; tcm-199 + poly vinyl alcohol (pva) and group-c; cr1aa + bsa. embryo production rate and expression level of insulin-like growth factor genes (igf-1, igf-1r, igf-2 and igf- ... | 2010 | 19392670 |
| effect of sodium nitroprusside, a nitric oxide donor, and aminoguanidine, a nitric oxide synthase inhibitor, on in vitro development of buffalo (bubalus bubalis) embryos. | 2010 | 19416489 | |
| cryotop vitrification of buffalo (bubalus bubalis) in vitro matured oocytes: effects of cryoprotectant concentrations and warming procedures. | the aim of the work was to evaluate the in vitro developmental competence of in vitro-matured buffalo oocytes after cryotop vitrification (ctv) and in vitro fertilization (ivf). to optimize parameters, two cryoprotectant (cp) concentrations and two warming-dilution procedures were applied. oocytes were vitrified in 16.5% ethylene glycol (eg), 16.5% dimethylsulphoxide (dmso) and 0.5 m sucrose in groups a and c, and in higher cp concentrations (20% eg, 20% dmso and 0.5 m sucrose) in groups b and d ... | 2010 | 19473307 |
| effects of exposure to heavy metals on viability, maturation, fertilization, and embryonic development of buffalo (bubalus bubalis) oocytes in vitro. | the aim of the present study was to examine the effect of heavy metals, cadmium and lead, on buffalo oocyte viability and in vitro development. oocytes were aspirated from ovaries of slaughtered buffaloes. only viable and metabolically active oocytes with more than three layers of cumulus cell layers and homogeneous ooplasm were selected. effects of nine concentrations (0, 0.005, 0.05, 0.5, 1.0, 1.5, 2.5, 5, and 10 microg/ml) of cadmium or lead on buffalo oocyte viability, morphological abnormit ... | 2010 | 19475365 |
| expression and immunohistochemical localization of the neonatal fc receptor (fcrn) in the mammary glands of the egyptian water buffalo. | although a marginal placental transfer of maternal immunoglobulin (ig) has been demonstrated in buffalo, the colostrum still provides the main source of immune components and nutrients to neonate buffalo calves. the neonatal fc receptor (fcrn) transports maternal ig across the gut wall and is involved in the transport of igg in the mammary gland. in this study we used rt-pcr to examine the gene expression of fcrn in the mammary gland during several physiological states of the egyptian water buff ... | 2010 | 19481783 |
| transplacental transfer of iron in the water buffalo (bubalus bubalis): uteroferrin and erythrophagocytosis. | the objectives of this investigation were to understand transplacental transport of iron by secreted uteroferrin (uf) and haemophagous areas of water buffalo placenta and clarify the role(s) of blood extravasation at the placental-maternal interface. placentomes and interplacentomal region of 51 placentae at various stages of gestation were fixed, processed for light and transmission electron microscopy, histochemistry and immunohistochemistry. haemophagous areas were present in placentomes coll ... | 2010 | 19453495 |
| effects of fsh and lh on steroid production by buffalo (bubalus bubalis) granulosa cells cultured in vitro under serum-free conditions. | the objective of this study was to examine the effects of fsh and lh on oestradiol-17β and progesterone production by buffalo granulosa cells cultured under serum-free conditions. granulosa cells (3 × 10(5) ) from small (≤ 5 mm diameter) follicles were cultured for up to 4 days in 48-well plates coated with 3.3 μg/cm(2) fibronectin in dulbecco's modified eagle's medium (dmem) : nutrient mixture f-12 ham (1 : 1 ratio) supplemented with 10(-7) m androstenedione, 5 μg/ml human apo-transferrin and 0 ... | 2010 | 19497023 |
| assessment of dna damage during in vitro development of buffalo (bubalus bubalis) embryos: effect of cysteamine. | comet assay was used in the present study to examine dna damage to buffalo oocytes and embryos during in vitro culture. embryos were produced in vitro from oocytes obtained from slaughterhouse ovaries in presence of cysteamine (ivm and ivc media supplemented with 50 and 100 μm, respectively) or in its absence (controls). compared to controls, cysteamine supplementation increased (p < 0.01) cleavage rate and proportion of oocytes that developed to 8- to 16-cell stage. the incidence of dna damage ... | 2010 | 19515031 |
| buffalo (bubalus bubalis) pre-antral follicle population and ultrastructural characterization of antral follicle oocyte. | the main objectives of the present study were to determine the ultrastructural modifications occurring in the oocyte during late folliculogenesis and to estimate pre-antral follicle population in buffalo. half the collected ovaries were fixed and prepared for optic microscopy; the antral follicles from the other ovaries were measured and individually punctured. the cumulus-oocyte complexes (cocs) were processed for transmission electron microscopy. the number of pre-antral follicles in buffalo o ... | 2010 | 19055556 |
| in vitro culture and differentiation of buffalo (bubalus bubalis) spermatogonia. | the objective of this study was to develop a culture system which could support buffalo spermatogonia differentiation into spermatids in vitro. testes from 3- to 5-month-old buffaloes were decapsulated and seminiferous tubules were enzymatically dissociated to recover spermatogonia and sertoli cells. the cells were cultured in modified dulbecco modified eagle medium supplemented with different concentrations of foetal bovine serum, retinol, testosterone for 2 months at 37 degrees c. spermatogoni ... | 2010 | 19090820 |
| induction of ovulation of ovulatory size non-ovulatory follicles and initiation of ovarian cyclicity in summer anoestrous buffalo heifers (bubalus bubalis) using melatonin implants. | this study was conducted on summer anoestrous buffalo heifers to monitor the efficacy of melatonin for induction of ovulation and ovarian cyclicity. during pre-treatment period of 24 days, the ovarian dynamics of five cycling and 10 summer anoestrous heifers was monitored on each alternate day using a transrectal ultrasound scanner. thereafter, during treatment period, these 10 anoestrous heifers along with additional seven anoestrous heifers were randomly allocated into non-implanted (n = 5) an ... | 2010 | 19090824 |
| effect of different combinations of cryoprotectants on in vitro maturation of immature buffalo (bubalus bubalis) oocytes vitrified by straw and open-pulled straw methods. | this study was designed to evaluate effects of different combinations of cryoprotectants on the ability of vitrified immature buffalo oocytes to undergo in vitro maturation. straw and open-pulled straw (ops) methods for vitrification of oocytes at the germinal vesicle stage also were compared. the immature oocytes were harvested from ovaries of slaughtered animals and were divided into three groups: (i) untreated (control); (ii) exposed to cryoprotectant agents (cpas); or (iii) cryopreserved by ... | 2010 | 19090828 |
| sarcocystis dubeyi (huong and uggla, 1999) infection in water buffaloes ( bubalus bubalis ) from egypt. | abstract water buffaloes ( bubalus bubalis ) are intermediate hosts for 4 species of sarcocystis , i.e., sarcocystis fusiformis and sarcocystis buffalonis with cats as definitive hosts; sarcocystis levinei with dogs as definitive hosts; and sarcocystis dubeyi with an unknown definitive host but thought to be zoonotic. currently, the latter species has been identified with certainty only from vietnam. in the present study, sarcocysts of s. dubeyi are reported in 11 (30%) of 35 egyptian water buff ... | 2010 | 21506768 |
| ultrasonographic and endocrine aspects of follicle deviation, and acquisition of ovulatory capacity in buffalo (bubalus bubalis) heifers. | the objectives of this study were to determine the interval from ovulation to deviation and the diameter of the dominant (df) and largest subordinate (sf) follicles at deviation in buffalo (bubalus bubalis) heifers. two methods of evaluation (observed vs. calculated) were used. fsh and lh profiles encompassing follicle deviation (experiment 1), and the follicular diameter when the df acquired ovulatory capacity (experiment 2) were also determined. the time of deviation and the diameter of the df ... | 2010 | 21256685 |
| clinicopathological and ultrasonographic findings in 40 water buffaloes (bubalus bubalis) with traumatic pericarditis. | forty buffaloes with traumatic pericarditis were examined to characterise the ultrasonographic findings in buffaloes with traumatic pericarditis, determine the extent of the lesions and assess the prognosis. the most noticeable clinical presentations were presternal oedema (73 per cent) and jugular and mammary vein distension (88 per cent). laboratory findings included neutrophilic leucocytosis, elevated total protein concentration, hypoalbuminaemia, hypergammaglobulinaemia and increased concent ... | 2010 | 21262631 |
| chronological reorganization of microtubules, actin microfilaments, and chromatin during the first cell cycle in swamp buffalo (bubalus bubalis) embryos. | this paper aimed to study the dynamics of early embryonic development, in terms of redistribution of cytoskeleton (microtubules, actin microfilaments) and chromatin configurations during the first cell cycle in swamp buffalo embryos. oocytes were matured and fertilized in vitro, and they were fixed at various time points after ivf. at 6 h after ivf, 44.4% matured oocytes were penetrated by spermatozoa. partial zp digestion, however, did not improve fertilization rate compared to control (p > .05 ... | 2010 | 21234419 |
| sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings. | in this study, we compared the morphology of sarcocystis sinensis and sarcocystis hominis, and assessed the infectiousness of s. sinensis for human volunteers. the cysts of s. sinensis were from water buffalo (bubalus bubalis) and those of s. hominis were from cattle (bos taurus). transmission electron microscopy of s. sinensis cysts revealed that the cyst wall had leaning, finger-like protrusions measuring 1.44-5.08μm in length and without invaginations on the tip surface of the protrusions. in ... | 2010 | 21236581 |
| allelic diversity at mhc class ii dq loci in buffalo (bubalus bubalis): evidence for duplication. | the genetic diversity of mhc class ii dq genes was investigated in riverine buffalo (bubalus bubalis) by pcr-rflp and sequencing. highly variable regions (exons 2-3) of dq genes were amplified from 152 buffaloes and genotyped by pcr-rflp. alleles identified by differential restriction patterns were sequenced for the characterization. pcr-rflp was a rapid method to discriminate between dqa1 and duplicated dqa2 genes in buffalo, however, the method appeared to be inadequate for determining the mor ... | 2010 | 20724005 |
| expression of pluripotency genes in buffalo (bubalus bubalis) amniotic fluid cells. | recent findings suggest that mammalian amniotic fluid (af) is a source of multipotent stem cells (scs), which can be used in regenerative medicine and assisted reproduction. we report the isolation, culture and characterization of amniotic fluid-derived cells from pregnant water buffalo uterus. these undifferentiated af cells expanded without feeder layer over a period of 100 days up to passages 20 and the expression of alkaline phosphatase (ap), oct-4, nanog and sox-2, gapdh and β-actin could b ... | 2010 | 21198969 |
| a global view of 54,001 single nucleotide polymorphisms (snps) on the illumina bovinesnp50 beadchip and their transferability to water buffalo. | the illumina bovinesnp50 beadchip features 54,001 informative single nucleotide polymorphisms (snps) that uniformly span the entire bovine genome. among them, 52,255 snps have locations assigned in the current genome assembly (btau_4.0), including 19,294 (37%) intragenic snps (i.e., located within genes) and 32,961 (63%) intergenic snps (i.e., located between genes). while the snps represented on the illumina bovine50k beadchip are evenly distributed along each bovine chromosome, there are over ... | 2010 | 21209788 |
| impact of pre-ovulatory follicle diameter on plasma estradiol, subsequent luteal profiles and conception rate in buffalo (bubalus bubalis). | the present study was designed to investigate the impact of pre-ovulatory follicle (pof) diameter on the day of estrus on plasma estradiol concentration, subsequent luteal profile (corpus luteum, cl, diameter and plasma progesterone concentration) and conception rate in buffaloes. twenty-eight buffaloes were synchronized with synthetic analogue of prostaglandin f(2α) (pgf(2α)) administered 11 days apart. transrectal ultrasonography and jugular vein blood sampling was carried out on the day of es ... | 2010 | 21215536 |
| growth, metabolic status and ovarian function in buffalo (bubalus bubalis) heifers fed a low energy or high energy diet. | the aim was to establish the capacity of buffalo heifers to adapt their metabolic requirements to a low energy diet. murrah buffalo (bubalus bubalis) heifers undergoing regular estrous cycles were randomly assigned by age, live weight (lw) and body condition score (bcs) to a high energy group (he, 5.8 milk forage units (mfu)/day, n=6) or low energy group (le, 3.6 mfu/day, n=6). circulating concentrations of metabolic substrates, metabolic hormones and reproductive hormones were determined weekly ... | 2010 | 20729015 |
| molecular characterization and expression profile of uterine serpin (serpina14) during different reproductive phases in water buffalo (bubalus bubalis). | uterine serpins (serpina14) play important roles during pregnancy in the farm animals. in this study, we have cloned and characterized cdna sequence encoding the bubaline serpina14. we also studied its spatio-temporal expression in the uterine endometrium. the bubaline serpina14 has an open reading frame of 1299bp. itshares ∼90% identity with the serpina14 of other ruminant livestock species. phylogenetically, bubaline serpina14 has been placed in the same clade that contained other mammalian ho ... | 2010 | 20801587 |
| stenotrophomonas maltophilia as a causal agent of pyogranulomatous hepatitis in a buffalo (bubalus bubalis). | a 7-year-old female buffalo (bubalus bubalis) from a local herd in serres, northern greece, was presented to a private veterinary clinic with a chronic loss of appetite for 15 days. the clinical examination revealed high fever (41.5 degrees c), lethargy, yellow discoloration of skin and mucous membranes, an abdomen that appeared to be empty, hyperactive rumen motility, and tachypnea. a biochemical profile revealed an elevated total bilirubin concentration and hepatic enzyme activities, whereas g ... | 2010 | 20807941 |
| reproductive cycles of buffalo. | the domestic water buffalo (bubalus bubalis) has an important role in the agricultural economy of many developing countries in asia, providing milk, meat and draught power. it is also used in some mediterranean and latin american countries as a source of milk and meat for specialized markets. although the buffalo can adapt to harsh environments and live poor quality forage, reproductive efficiency is often compromised by such conditions, resulting in late sexual maturity, long postpartum anoestr ... | 2010 | 20869822 |
| immunolocalization of pulmonary intravascular macrophages, tlr4, tlr9 and il-8 in normal and pasteurella multocida-infected lungs of water buffalo (bubalus bubalis). | water buffalo are of considerable economic significance in south east asia, but these animals suffer from many bacterial respiratory diseases including haemorrhagic septicaemia caused by pasteurella multocida. bacterial respiratory diseases of animals cause lung inflammation that is characterized by the activation of toll-like receptors (tlrs) expressed on macrophages, expression of chemokines and recruitment of neutrophils and monocytes. pulmonary intravascular macrophages (pims) present in the ... | 2010 | 20880542 |
| dynamic in vivo changes in the activities of gelatinases, matrix metalloproteinases (mmps), and tissue inhibitor of metalloproteinases (timps) in buffalo (bubalus bubalis) uterine luminal fluid during estrous cycle and early pregnancy. | in ruminants, the phenomenon of endometrial tissue remodeling during the estrous cycle and early pregnancy is not fully understood. in this report, the occurrence of tissue remodeling, if any, in buffalo endometrium was studied by detecting gelatinases, matrix metalloproteinases (mmps) and tissue inhibitors of metalloproteinases (timps); the key regulators of tissue remodeling, in uterine luminal fluids (ulf) of cycling and early pregnant (approx. 43-65 days) buffaloes. each stage of the estrous ... | 2010 | 20886603 |
| eating and rumination behaviour in brahman grade cattle and crossbred water buffalo fed on high roughage diet. | the purpose of this study is to compare feeding and other behavior and nutrient digestibility of tropical grade brahman (body weight (bw) = 231 kg ± 12.4; n = 3) and crossbred water buffalo (bw = 300 kg ± 13.9; n = 3). this experiment on digestibility and measures of muscles of mastication utilized one-way, and animal behavior two-way, analysis of variance, respectively. two video camcorders were installed in each pair of buffalo and brahman for 24 h period programmed on the 107th, 109th and 111 ... | 2010 | 20887310 |
| seroprevalence of toxoplasma gondii in water buffaloes (bubalus bubalis) in south-west of iran. | the prevalence of antibodies to toxoplasma gondii was conducted in 300 buffaloes from ahvaz, kouzestan province, southwest of iran. blood sera were screened using a modified agglutination test (mat) incorporating 2-mercaptoethanol. positive reactions in sera dilutions above 1:25 were considered as indicative for the presence of t. gondii antibodies. the overall prevalence of infection in the animals was 14.33% with titers of 1:25 in 21, 1:50 in 12, 1:100 in 6, 1:200 in 2 and 1:400 in 2. the prev ... | 2010 | 20962725 |
| sarcocystis cruzi: comparative studies confirm natural infections of buffaloes. | controversy exists concerning whether cattle and water buffalo sustain infections with cysts of distinct arrays of species in the genus sarcocystis. in particular, morphologically similar parasites have been alternately ascribed to sarcocystis cruzi or to sarcocystis levinei, depending on their occurrence in cattle or water buffalo. we used light and transmission electron microscopy, genetic analysis, and experimental infections of definitive canine hosts to determine whether consistent differen ... | 2010 | 20971107 |
| virulence of brucella abortus isolated from cattle and water buffalo. | brucellosis has been documented in domestic water buffalo (bubalus bubalis) but published literature is limited despite the importance of this species in tropical agricultural systems. the objective of this study was to compare the virulence of brucella abortus isolates recovered from cattle and water buffalo. nineteen strains of b. abortus from cattle and domestic water buffalo in trinidad were intraperitoneally inoculated into balb/c mice. spleens were cultured for b. abortus and histopatholog ... | 2010 | 20972706 |
| bilateral follicular cysts in a water buffalo. | the present short communication puts on record a case of bilateral, multiple follicular cysts in a water buffalo along with a detailed description of its ovarian biometry and follicular fluid composition. the ovarian weight and biometrical parameters were much higher than in normal cycling buffaloes. a total of three follicular cysts were observed, two on the right ovary and one on the left ovary, measuring 4.9, 3.0 and 2.6 cm yielding 21, 9 and 5 ml of follicular fluid, respectively. the cystic ... | 2010 | 21086160 |
| experimental infection of dogs (canis familiaris) with sporulated oocysts of neospora caninum. | neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. dogs and coyotes are definitive hosts of n. caninum and several species of domestic and wild animals are intermediate hosts. dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. it is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of n. caninum oocysts in feces. the objective of ... | 2010 | 21094584 |
| temporal changes in circulating levels of plasma interleukin-8 during peripartum period in murrah buffaloes (bubalus bubalis) under subtropical climate. | the objective of this study was to elucidate the changes in circulating levels of plasma interleukin-8 (il-8) during peripartum period in murrah buffaloes (bubalus bubalis). il-8 was estimated in blood plasma of healthy peripartum murrah buffaloes (n=6) on days ± 30, ± 15, ± 5, ± 3, ± 1 and 0 pre- and postpartum with respect to the day of parturition (day 0) in each of the two different seasons (hot-humid and spring). the mean microclimate temperature-humidity index (thi) during spring season wa ... | 2010 | 21107908 |
| selection of possible marker peptides for the detection of major ruminant milk proteins in food by liquid chromatography-tandem mass spectrometry. | the aim of this work was the determination of peptides, which can function as markers for identification of milk allergens in food samples. emphasis was placed on two casein proteins (α- and β-casein) and two whey proteins (α-lactalbumin and β-lactoglobulin). in silico tryptic digestion provided preliminary information about the expected peptides. after tryptic digestion of four milk allergens, the analytical data obtained by combination of reversed-phase high performance liquid chromatography a ... | 2010 | 21107975 |