Publications

isolation, sequencing and expression of bartonella henselae omp43 and predicted membrane topology of the deduced protein.	the infection of and interaction of human endothelial cells with bartonella henselae is one of the most interesting aspects of bartonella -associated disease. the gene encoding the 43 kda b. henselae outer membrane protein (omp43) that binds endothelial cells was cloned and sequenced. sequence analysis revealed an open reading frame of 1206 nucleotides coding for a protein of 402 amino acids. analysis of the deduced amino acid sequence shows 38% identity over the entire sequence to the brucella ...	2000	10906262
seroprevalence of bartonella henselae and toxoplasma gondii among healthy individuals in thailand.	the seroprevalence of bartonella henselae and toxoplasma gondii among apparently healthy individuals, mainly blood donors, in thailand was investigated by an indirect fluorescent antibody technique and by a latex agglutination test, respectively. of 163 serum samples examined, 9 (5.5%) were found to be positive for b. henselae-igg, 2 (1.2%) for b. henselae-igm, and 5 (3.1%) for the t. gondii antibody. no significant difference was observed between male and female samples in the serological test ...	2000	10907691
hemin-dependent growth and hemin binding of bartonella henselae.	bartonella henselae causes cat-scratch disease and bacillary angiomatosis peliosis. the bacteria reside in erythrocytes of asymptomatic cats, which represent the natural reservoir for this pathogen. b. henselae is usually grown on blood-enriched media. growth experiments on brucella medium without blood demonstrated that heme compounds are essential for the growth of b. henselae and can completely substitute the addition of blood components. the heme precursor protoporphyrin ix alone, or in comb ...	2000	10913865
infectious exanthems and unusual infections.	invasive disease due to group a beta-hemolytic streptococci (gabhs) can be divided into 3 categories of disease: streptococcal toxic shock syndrome (streptss), necrotizing fasciitis, and other invasive gabhs disease. patients with streptss may have multiorgan failure within hours of presentation. clindamycin and penicillin g should be used in combination for treatment of invasive gabhs disease. the mortality rate for menstrual staphylococcal toxic shock syndrome has decreased with early recognit ...	2000	10916128
[a "diagnosis of goldsmith"].		2000	10916845
are bartonella emerging and re-emerging pathogens in southern africa?		2000	10918880
characterization of bartonella clarridgeiae flagellin (flaa) and detection of antiflagellin antibodies in patients with lymphadenopathy.	cat scratch disease (csd) is a frequent clinical outcome of bartonella henselae infection in humans. recently, two case reports indicated bartonella clarridgeiae as an additional causative agent of csd. both pathogens have been isolated from domestic cats, which are considered to be their natural reservoir. b. clarridgeiae and b. henselae can be distinguished phenotypically by the presence or absence of flagella, respectively. separation of the protein content of purified flagella of b. clarridg ...	2000	10921956
neuroretinitis associated with cat scratch disease in a child.		2000	10933777
[update in infectious diseases. part i: epidemiology].	a number of infectious agents has been newly detected in the last 10 years. climatic changes and migration have been the most important factors in the emergence of new and old infections. additionally, new methods for the detection of dna and rna have played an important role in the detection of agents difficult to culture. relevant new bacterial pathogens are bartonella henselae (cat scratch disease, bacillary angiomatosis), tropheryma whippeli (whipple's disease) and new rickettsiae. newly det ...	2000	10935415
molecular cloning, sequencing, expression, and characterization of an immunogenic 43-kilodalton lipoprotein of bartonella bacilliformis that has homology to nlpd/lppb.	a recombinant clone expressing an immunoreactive antigen of bartonella bacilliformis was isolated by screening a genomic dna library with serum from a patient with the chronic verruga phase of bartonellosis. the clone, pbipim-17, contained a partial open reading frame that expressed an immunoreactive fusion protein. subsequent rescreening of the library by plaque hybridization resulted in the isolation of recombinant clones that contain the entire open reading frame. the open reading frame (orf- ...	2000	10948113
[polymerase chain reaction (pcr) for microbiological diagnosis in refractory infectious keratitis: a clinical study in 16 patients].	the identification of the causative pathogen in infectious keratitis is possible in only 60% of the cases. the aim of this study was to show if this number increases by the use of pcr.	2000	10949815
[evaluation of serological response to bartonella henselae by enzyme immunoassay in cat scratch disease].	the igg and igm titers to bartonella henselae were determined by an enzyme immunoassay (eia). the eia test for detection of igg and igm antibodies to b. henselae concerning csd showed that 8 (40%) of 20 patients with csd had a serum igg antibody titer of 12 eia unit or more and that 5 (25%) patients had a serum igm titer of 12 eia unit or more. totally 12 (60%) of the 20 patients with csd were seropositive for b. henselae. the mean age of igg positive patients were higher than igm positive patie ...	2000	10965658
extracellular bartonella henselae and artifactual intraerythrocytic pseudoinclusions in experimentally infected cats.	blood, spleen and liver of specific pathogen-free (spf) cats and spf cats experimentally infected with bartonella henselae were examined. using immunohistochemical labeling, no intracellular b. henselae were observed in tissues of any cats, but extracellular b. henselae were detected in tissues of infected cats. pseudoinclusions were detected in erythrocytes of all cats using electron microscopy.	2000	10973702
[familial parinaud oculo-glandular syndrome in cat-scratch disease].	cat-scratch disease is manifested by subacute, regional lymphadenitis and occurs mainly in children. the causative agent is a pleomorphic, gram-negative bacillus, bartonella henselae carried by asymptomatic cats. parinaud oculoglandular syndrome is the most common ocular manifestation of this disease. it is characterized by unilateral conjunctivitis with polypoid granuloma, usually of the palpebral conjunctiva, and preauricular lymphadenopathy. the diagnosis is supported by a history of exposure ...	2000	10979428
transverse myelitis associated with probable cat-scratch disease in a previously healthy pediatric patient.	we evaluated a pediatric patient for transverse myelitis associated with bartonella henselae infection. there was no adenopathy in our patient, but the diagnosis was made serologically. it is necessary to keep cat-scratch disease in mind even in the absence of typical findings.	2000	10987731
transcriptional activation of the htra (high-temperature requirement a) gene from bartonella henselae.	bacterial htra genes are typically activated as part of the periplasmic stress response and are dependent on the extracytoplasmic sigma factor rpoe. a putative promoter region, p1, of the sigma(e)-type heat-inducible promoters has previously been identified upstream of the htra gene of bartonella henselae. further analysis of the htra mrna by primer extension demonstrated that transcription initiates from p1 and a second region downstream of p1. this second promoter region, termed p2, had no seq ...	2000	10992509
[neonatal papular acrodermatitis (gianotti-crosti) and bartonella henselae infection].		2000	11003916
bartonella henselae associated uveitis and hla-b27.	to investigate the frequency of hla-b27 in patients with presumed bartonella henselae associated uveitis and to describe the clinical characteristics of hla-b27 positive patients with uveitis and presumed ocular bartonellosis (pob).	2000	11004097
[parotid involvement in cat scratch disease: a differential diagnosis with increased significance].	bartonella henselae is the causative agent of cat-scratch disease (csd), an inflammatory infection of the lymph nodes. so far, only few cases of atypical manifestations in the head and neck, especially manifestations in the parotid gland have been reported.	2000	11006911
bartonella henselae, b. quintana, and b. bacilliformis: historical pathogens of emerging significance.	bartonella species were virtually unrecognized as modern pathogens of humans until the last decade. however, identification of bartonella species as the agents of cat-scratch disease, bacillary angiomatosis, urban trench fever, and possible novel presentations of carrion's disease has left little doubt of the emerging medical importance of this genus of organisms. the three primary human pathogenic bartonellae, bartonella bacilliformis (carrion's disease), b. henselae (cat-scratch disease), and ...	2000	11008109
regional lymphadenopathy in cat-scratch disease: ultrasonographic findings.	cat-scratch disease (csd) is considered to be an emerging disease worldwide and is caused by bartonella henselae, a gram-negative bacterium introduced by a scratch or bite of a cat. the most common clinical manifestation is regional lymphadenopathy, but clinical recognition may be difficult, as atypical manifestations may occur. the diagnosis is confirmed with serologic testing and histology is rarely needed. this paper is based on our experience with the use of ultrasonography in the diagnosis ...	2000	11009305
optic disk edema associated with peripapillary serous retinal detachment: an early sign of systemic bartonella henselae infection.	to describe optic disk edema associated with peripapillary serous retinal detachment as an early sign of systemic bartonella henselae infection.	2000	11020412
ocular bartonellosis.	to review recent advances in the basic and clinical biology of bartonella-related eye disease.	2000	11020414
pseudoinfectious mononucleosis: a presentation of bartonella henselae infection.	six children presented during one year with clinical features of infectious mononucleosis, but with laboratory findings of leucocytosis with neutrophilia, increased erythrocyte sedimentation rate, and hypergammaglobulinaemia. serology for epstein-barr virus, cytomegalovirus, adenovirus, and toxoplasma gondii was negative, while anti-bartonella henselae igm with high igg titre (>/=1/1024) was present in all. all children had contact with kittens. no specific treatment was administered and all rec ...	2000	11040157
rickettsia prowazekii and bartonella henselae: differences in the intracellular life styles revisited.	within the alpha subdivision of proteobacteria, the arthropod-borne human pathogens rickettsia prowazekii and bartonella henselae provide examples of bacteria with obligate and facultative intracellular life styles, respectively. the complete genome sequence of r. prowazekii has been published, whereas the sequencing of the b. henselae genome is in its final stage. here, we provide a brief overview of a comparative analysis of both genomes based on the delineated metabolic properties. the relati ...	2000	11045918
[peripheral lymphadenopathy in childhood--recommendations for diagnostic evaluation].	enlargement of peripheral lymph nodes most commonly caused by a local inflammatory process is frequently seen in childhood. the aim of the present study was to analyze the most common causes of peripheral lymphadenopathy and to develop a simple algorithm for the primary diagnostic evaluation of peripheral lymph node enlargement in this age group.	2000	11048289
immune aspects of bartonella.	bartonella species have been recognized as important human pathogens only recently. until the early 1990s, this genus was represented by one species, bartonella bacilliformis. the recent identification of other bartonella species as the agents of cat-scratch disease and bacillary angiomatosis has left little doubt of their emerging importance as opportunistic human pathogens. over the last decade, extensive research has been performed on bartonella species, resulting in an explosion in our knowl ...	2000	11052646
[when cats scratch].		2000	11059029
failure to detect bartonella henselae infection in synovial fluid from sufferers of chronic arthritis.	bartonella henselae causes granulomatous and indolent infection in the immune competent human, and angioproliferation in the context of persistent infection and impaired immunity. this bacterium is found in up to 40% of household cats, from which humans acquire it by either a cat scratch or a bite (hence the name, cat-scratch disease). approximately 5% of australian and us blood donors have serological evidence of past infection, but most associated illnesses are mild or subclinical. a number of ...	2000	11063291
bartonella henselae infection in british columbia: evidence for an endemic disease among humans.	human bartonellosis in north america is mainly associated with bartonella henselae, and the availability of laboratory diagnostic tools has significantly heightened awareness of the spectrum of human disease that is caused by this bacterium. we detail herein examples of illness in a pediatric population which serve to confirm that b. henselae-associated disease exists in british columbia. seroprevalence studies among asymptomatic adults and among children with symptomatic respiratory illness of ...	2000	11068677
disciforme keratitis caused by bartonella henselae: an unusual ocular complication in cat scratch disease.	cat scratch disease (csd) is a common infectious disease, however, its association with disciforme keratitis is a previously unreported ocular complication. with the use of the 16s rdna-pcr technique with subsequent dann sequencing on corneal material obtained by corneal scrape we were able to identify bartonella henselae in an unusual form of disciforme keratitis.	2000	11071035
[bacillary angiomatosis of the liver, a suspected ultrasound diagnosis?].	infections with bartonella henselae or bartonella quintana present with vasoproliferative lesions in skin and parenchymatous organs in immunocompromised patients. a case report of a 38-year-old patient with hiv infection and hepatitis b surface antigen status is described. the dominant clinical symptoms in our patient were fever and icterus. ultrasonography of the abdomen showed a picture similar to that of liver cirrhosis. irregular (echorich) nodes with hyper-vascularization were suspected to ...	2000	11072674
bartonella henselae and inflammatory bowel disease.	sustained fever and increased thickness of the distal ileum on ultrasound suggested crohn's disease in an adolescent boy. bartonella henselae infection was diagnosed by specific serology and the patient recovered. ileitis could be related to b. henselae infection.	2000	11072951
images in clinical medicine. bartonella neuroretinitis.		2000	11078771
hemin-binding surface protein from bartonella quintana.	bartonella quintana, the agent of trench fever and a cause of endocarditis and bacillary angiomatosis in humans, has the highest reported in vitro hemin requirement for any bacterium. we determined that eight membrane-associated proteins from b. quintana bind hemin and that a approximately 25-kda protein (hbpa) was the dominant hemin-binding protein. like many outer membrane proteins, hbpa partitions to the detergent phase of a triton x-114 extract of the cell and is heat modifiable, displaying ...	2000	11083791
[neck abscess as primary manifestation of head and neck carcinoma: implications for diagnostic management].	neck abscesses and deep neck infections are common diseases in the field of otorhinolaryngology. however, malignant lymph node metastases presenting as abscesses are uncommon and have rarely been described up to now.	2000	11089210
recent advances in diagnosis and treatment of cat scratch disease.	the cause of cat scratch disease (csd), first described in france in 1950 and in the united states in 1951, was unknown until 1983 when the bacterium in lymph nodes was detected using a warthin-starry silver stain. afipia felis has been an infrequent cause of csd since1988, when this gram-negative bacterium was first isolated from 10 patients with csd. in 1992 bartonella organisms were isolated from immunocompetent and immunocompromised patients. an indirect fluorescent antibody test to detect b ...	2000	11095849
detection of serum antibodies to bartonella henselae and coxiella burnetii from japanese children and pregnant women.	the participation of bartonella henselae and coxiella burnetii in the pathogenesis of fever of unknown origin (fuo) and lymphadenopathy has not been completely clarified. prevalence of these two agents in japanese children is also unknown. serum igg and igm antibodies to b. henselae and to c. burnetii were examined by the indirect fluorescence antibody assay. enzyme immunoassay kits were used to detect serum igg and iga antibodies against chlamydia trachomatis. out of 200 healthy normal pregnant ...	2000	11099929
species-specific monoclonal antibodies for rapid identification of bartonella quintana.	seven species-specific monoclonal antibodies (mabs) to bartonella quintana were produced and characterized. the mabs were of the immunoglobulin g class and reacted only with 13 b. quintana strains in indirect microimmunofluorescence and western immunoblotting assays. they did not react with eight other bartonella spp., including bartonella henselae, the most closely related species, and a selected mab did also not react with nine other strains of gram-negative bacteria. the mabs reacted mainly w ...	2000	10618271
bacillary angiomatosis by bartonella quintana in an hiv-infected patient.	bacillary angiomatosis and bacillary peliosis are opportunistic infections caused by bartonella henselae and bartonella quintana, which occur in patients with late-stage infection. we report a case of bacillary angiomatosis in an hiv-infected patient with skin, bone, and probably liver involvement, the identification of the agent (b quintana ) was done by polymerase chain reaction in the skin specimen. the patient had complete regression of all lesions after a 6-month regimen of oral erythromyci ...	2000	10642694
sequence variation in the ftsz gene of bartonella henselae isolates and clinical samples.	in a search for methods for subtyping of bartonella henselae in clinical samples, we amplified and sequenced a 701-bp region in the 3' end of the ftsz gene in 15 b. henselae isolates derived from cats and humans in the united states and europe. the ftsz sequence variants that were discovered were designated variants bh ftsz 1, 2, and 3 and were compared with 16s rrna genotypes i and ii of the same isolates. there was no ftsz gene variation in the strains of 16s rrna type i, all of which were bh ...	2000	10655367
cloning, sequencing, and expression of three bartonella henselae genes homologous to the agrobacterium tumefaciens virb region.	a 17-kda, immunodominant antigen of bartonella henselae houston-1 has previously been cloned, sequenced, and characterized. this clone (h13) contains the 17-kda antigen gene plus a partial open reading frame, designated orf1, which is 459 nucleotides long and is directly upstream of the 17-kda gene. comparison of the deduced partial amino acid sequence of orf1 with that of other known genes in genbank revealed significant identity with several other bacterial virulence genes, including virb4 of ...	2000	10749166
prevalence of bartonella henselae, bartonella clarridgeiae and the 16s rrna gene types of bartonella henselae among pet cats in japan.	the authors investigated bacteriologically the prevalence of bartonella infection among 690 pet cats derived from 10 private animal hospitals in six cities (sapporo, hokkaido prefecture; sendai, miyagi prefecture; joetsu, niigata prefecture; fujisawa, kanagawa prefecutre; kyoto, kyoto prefecture; sanda, hyogo prefecutre) and 4 counties (mishima, osaka prefecture; hikawa, shimane prefecture; aira, kagoshima prefecture; shimajiri, okinawa prefecture) located from the north to the south of japan. b ...	2000	10770599
in vitro activities of telithromycin (hmr 3647) against rickettsia rickettsii, rickettsia conorii, rickettsia africae, rickettsia typhi, rickettsia prowazekii, coxiella burnetii, bartonella henselae, bartonella quintana, bartonella bacilliformis, and ehrlichia chaffeensis.	in vitro activities of telithromycin compared to those of erythromycin against rickettsia spp., bartonella spp., coxiella burnetii, and ehrlichia chaffeensis were determined. telithromycin was more active than erythromycin against rickettsia, bartonella, and coxiella burnetii, with mics of 0.5 microg/ml, 0.003 to 0.015 microg/ml, and 1 microg/ml, respectively, but was inactive against ehrlichia chaffeensis.	2000	10770788
clinical disease in kittens inoculated with a pathogenic strain of bartonella henselae.	to evaluate disease in kittens inoculated with bartonella henselae strain lsu16.	2000	10772100
visceral manifestation of cat scratch disease in children. a consequence of altered immunological state?	a 12-year-old girl with a 2-month history of fever and abdominal pain was admitted to our hospital. ultrasound and ct scans of the abdomen showed multiple hypoechoic lesions of liver and spleen. screening for zoonosis revealed high positive titers to bartonella henselae. t-cell deficiency was demonstrated and remained almost unchanged during a follow-up of 11 months. a review of the literature shows that disseminated visceral affection is a rare presentation of cat scratch disease (csd) in child ...	2000	10782400
rapid identification and differentiation of bartonella species using a single-step pcr assay.	five species of bartonella have been reported to infect humans and cause a variety of diseases that can be difficult to diagnose. four species of bartonella have been reported to infect cats and dogs, and two of these species are considered to be zoonotic pathogens. diagnosis of bartonella infections is hampered by the slow, fastidious growth characteristics of bartonella species. we report on the development of a single-step pcr-based assay for the detection and differentiation of medically rel ...	2000	10790087
bartonella henselae infection as a cause of fever of unknown origin.	fourteen of 41 patients (34%) with a serological diagnosis of bartonella henselae infection were found to have prolonged fever or fever of unknown origin, suggesting that generalized systemic b. henselae infection is not rare in immunocompetent healthy individuals.	2000	10790137
cat scratch disease: posterior segment manifestations.		2000	10811065
prevalence of serologic evidence of cat scratch disease in patients with neuroretinitis.	to determine the prevalence of bartonella henselae seropositivity in patients with a clinical diagnosis of neuroretinitis.	2000	10811077
prevalence of bartonella henselae antibody in florida panthers.	serum samples from 28 free-ranging florida panthers (puma concolor coryi) and seven mountain lions from texas (p. concolor stanleyana) living in south florida (usa) between 1997 to 1998 were tested for antibodies to bartonella henselae. twenty percent (7/35) of the samples were reactive to b. henselae antisera with a subspecies prevalence of 18% (5/ 28) for florida panthers and 28% (2/7) for cougars from texas (usa). there was not a significant sex related difference in infection rates among the ...	2000	10682759
peliosis hepatis in a dog infected with bartonella henselae.	a 6-year-old spayed female golden retriever was examined because of generalized weakness and abdominal distention. abdominal ultrasonography revealed a large quantity of peritoneal fluid. in addition, the liver appeared larger than normal and contained multiple, small, nodular masses and cyst-like structures. abdominal exploratory surgery was performed, and 5 l of serosanguineous peritoneal fluid was removed. gross lesions were not found in the stomach, kidneys, intestines, adrenal glands, or ur ...	2000	10687006
conservation of the 17-kilodalton antigen gene within the genus bartonella.	the 17-kda antigen of bartonella henselae has previously been shown to elicit a strong humoral immune response in patients with cat scratch disease (csd) and to be useful in screening human serum samples for csd. in this study, pcr amplification of genes homologous to the 17-kda antigen gene of b. henselae was performed using genomic dnas from several species of bartonella, including the currently recognized human pathogens. amplicons of similar size were demonstrated using the following chromos ...	2000	10702501
in vitro susceptibilities of rickettsia and bartonella spp. to 14-hydroxy-clarithromycin as determined by immunofluorescent antibody analysis of infected vero cell monolayers.	the in vitro susceptibilities of rickettsia akari, rickettsia conorii, rickettsia prowazekii, rickettsia rickettsii, bartonella elizabethae, bartonella henselae and bartonella quintana to different concentrations of clarithromycin, 14-hydroxy-clarithromycin (the primary metabolite of clarithromycin) and tetracycline in vero cell cultures, were determined by enumeration of immunofluorescently-stained bacilli. the extent of antibiotic-induced inhibition of foci was recorded for each dilution of an ...	2000	10702548
a bacteriophage-like particle from bartonella bacilliformis.	bartonella bacilliformis and bartonella henselae, the respective agents of oroya fever and cat-scratch disease in humans, are known to produce bacteriophage-like particles (blps) that package 14 kbp segments of the host chromosome. data from this study suggest that other bartonella species including bartonella quintana, bartonella doshiae and bartonella grahamii also contain similar blps, as evidenced by the presence of a 14 kbp extrachromosomal dna element in their genomes, whereas bartonella e ...	2000	10746763
polymerase chain reaction detection of bartonella henselae bacteraemia in an immunocompetent child with cat-scratch disease.	a case of bartonella henselae bacteraemia is reported in an immunocompetent 8-year-old boy with cat-scratch disease. serology to b. henselae, diagnosed by polymerase chain reaction, was positive. dna was extracted from peripheral whole blood and amplified with specific primers targeting the htra gene of b. henselae. a non-isotopic hybridization assay with a species-specific oligonucleotide probe was used to detect the amplified product. conclusion: the polymerase chain reaction can be used for t ...	2000	10834521
isolation in endothelial cell cultures of chlamydia trachomatis lgv (serovar l2) from a lymph node of a patient with suspected cat scratch disease.	an inguinal lymph node, removed from a 21-year-old romanian man suspected of having cat scratch disease, was sent to our laboratory for bartonella culture. lymph node specimens were inoculated on blood-enriched agar and in an endothelial cell culture system using the centrifugation shell vial technique. bacteria were grown in cell monolayers and detected as positive with an anti-bartonella henselae rabbit serum. however, such bacteria were identified as chlamydia trachomatis biovar lgv serovar l ...	2000	10834954
[clinical evaluation of commercial serological test for bartonella infection].	we evaluated the usefulness of a serological diagnostic kit (bartonella ifa igg, igm; mrl diagnostics) for bartonella henselae infection. of the 110 healthy individuals, 107 (97.3%) were with titers being less than 1:64 for igg antibody to b. henselae, 2 were with titers being 1:64 and 1 with 1:128, igm antibody to b. henselae was negative in all individuals. serological diagnosis of cat scratch disease (csd) using indirect fluorescence antibody (ifa) methods (in-house and diagnostic kit) was ma ...	2000	10835846
endotoxin and nanobacteria in polycystic kidney disease.	microbes have been suspected as provocateurs of polycystic kidney disease (pkd), but attempts to isolate viable organisms have failed. bacterial endotoxin is the most often reported microbial product found in pkd fluids. we assessed potential microbial origins of endotoxin in cyst fluids from 13 pkd patients and urines of pkd and control individuals.	2000	10844606
quantitative analysis of valvular lesions during bartonella endocarditis.	cardiac valve pathology was evaluated in 15 patients with confirmed diagnosis of bartonella endocarditis. ten were infected by bartonella quintana and 5 by bartonella henselae. histologic features of these cases, including fibrosis, calcification, vegetation, pattern of inflammation, and vascularization, were compared with those of valves from 25 cases of non-bartonella endocarditis as controls using a computerized quantitative image analysis. pathologic and immunohistologic testing for localiza ...	2000	11338477
cat-scratch disease.	cat-scratch disease (csd) was first described by debré in 1950, yet the causative bacterial agent of csd remained obscure until 1992, when bartonella (formerly rochalimaea) henselae was implicated in csd by serological and microbiologic studies. bartonella henselae had initially been linked to bacillary angiomatosis (ba), a vascular proliferative disease most commonly associated with long-standing human immunodeficiency virus infection or other significant immunosuppression. bartonella henselae ...	2000	11189710
determination of the rpob gene sequences of bartonella henselae and bartonella quintana for phylogenic analysis.	using the genome walker procedure, which allows pcr amplification of genomic dna using a single gene-specific primer and direct automated sequencing methodology, we obtained the nucleotide sequence of the rna polymerase beta subunit (rpob) from bartonella henselae and bartonella quintana. a phylogenetic tree constructed from these data and other rpob sequences available in genbank is, in part, consistent with those previously derived from 16s rrna gene sequences and confirms the position of bart ...	2000	11191808
three cases of cat scratch disease diagnosed by indirect immunofluorescence antibody assay and/or polymerase chain reaction of 16s rrna gene of bartonella henselae.	three suspected cases of cat scratch disease were diagnosed by indirect immunofluorescence antibody assay and/or polymerase chain reaction. patient 1 was a 10-year-old female who presented swelling of the right axillary [corrected] lymph nodes with pain and fever. she kept a kitten, and many scratches were observed on her both legs and dorsum manus. antibody titers against bartonella (b.) henselae were 1:32 for igm 3 weeks after the onset of the symptoms and 1:64 for igg 8 weeks after the onset. ...	2000	11193351
cervical lymphadenitis and cat scratch disease (csd): an overlooked disease?	cat scratch disease (csd) is a benign disease characterized by regional lymphadenopathy affecting most frequently the head and neck region in children and young adults. in the present paper, the authors describe four cases of csd focusing on clinical history, diagnostic management and therapy. the main germ responsible for this lymph node disease is bartonella henselae. diagnosis is based on history, serology and histological findings. clinical evolution is generally favorable despite the fact t ...	2000	11205454
detection of bartonella henselae and afipia felis dna by polymerase chain reaction in specimens from patients with cat scratch disease.	polymerase chain reaction (pcr) amplification and colorimetric identification of amplicons were performed to detect bartonella henselae and afipia felis dna in specimens from patients who were clinically and histologically suspected of having cat scratch disease. pcr products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (deia) (gen-eti-k; diasorin, italy). six paraffin-embedded lymph node ...	2000	11205638
interaction of bartonella henselae with endothelial cells results in rapid bacterial rrna synthesis and replication.	bartonella henselae is a slow-growing microorganism and the causative pathogen of bacillary angiomatosis in man. here, we analysed how interaction of b. henselae with endothelial cells might affect bacterial growth. for this purpose, bacterial rrna production and ribosome content was determined by fluorescence in situ hybridization (fish) using rrna-targeted fluorescence-labelled oligonucleotide probes. b. henselae grown on agar plates showed no detectable rrna content by means of fish, whereas ...	2000	11207598
[cat-scratch disease].	we report a case of fourteen years old boy with cat scratch disease. he was treated at pediatric clinic in january and february 2000. cat scratch disease is benign infectious chronic disease which appears in all ages. it begins after contact with cat and its scratch. yearly incidence of this disease for whole population is 0.77-0.86/100.000. the cause is bacillus bartonella which is also called genus rochalimaea. it is gram negative, mobile, aerobe bacillus with ten flagellas on one pole. our pa ...	2000	11219917
evidence of bartonella henselae infection in cats and dogs in the united kingdom.	sera from cats and dogs in the uk were tested by elisa for antibodies to bartonella henselae. seropositivity was confirmed in 28 of 69 pet cats (40.6 per cent), 33 of 79 feral cats (41.8 per cent) and three of 100 pet dogs. reactivity to specific b. henselae antigens was confirmed by western blotting and demonstrated that consistent antigenic bands were bound by sera from the cats and dogs.	2000	11132671
neuroretinitis: a clinical syndrome of cat-scratch disease.	cat-scratch disease is usually a benign self-limited illness, characterized by regional lymphadenopathy lasting between 3 and 6 weeks. the causative organism is bartonella henselae, a small gram-negative rod. between 1 and 2% of patients who contract the illness experience blurred vision, metamorphopsia and scotomas as a result of neuroretinitis, an associated clinical syndrome. the classical clinical findings in cat-scratch neuroretinitis include disc edema and a stellate pattern of exudates in ...	2000	11137430
diagnosis and treatment of bartonella henselae infections.		2000	11144381
[disciform keratatis caused by bartonella henselae infection: detection of a rare ocular complication of cat-scratch disease with pcr].	cat scratch disease (csd) is a common infectious disease, however its association with a disciforme keratitis is to our knowledge a previously unreported ocular complication.	2000	11146830
osteomyelitis of the hip joint associated with systemic cat-scratch disease in an adult.	reported here is the case of a 29-year-old male with cervical lymphadenopathy, fever and weight loss, followed by acute painful osteomyelitis of the left hip joint due to cat-scratch disease. the diagnosis was established by detection of igg antibodies to bartonella henselae in serum and histologic examination of a lymph node including a positive polymerase chain reaction test. treatment consisted of clarithromycin and cefotiam for 2 weeks. four weeks after discharge, all of the patient's sympto ...	2000	11117643
isolation of bartonella henselae from a serologically negative cat in bloemfontein, south africa.	sera collected from apparently healthy 6-12-month-old cats (n = 31) presented to the society for the prevention of cruelty to animals veterinary clinic in bloemfontein for neutering were tested for antibodies reactive to bartonella henselae (houston-1 strain) by indirect fluorescent antibody testing. whole blood collected from the cats was used in isolation experiments and subsequent identification of bartonella species was based on comparison of the nucleotide base sequence of polymerase chain ...	1999	10855838
hhv-8 (kshv) is not associated with bacillary angiomatosis.	bacillary angiomatosis is a rare pseudoneoplastic angioproliferative lesion occurring in patients with aids. this condition has been associated with bartonella henselae and bartonella quintana infections. human herpesvirus 8 (hhv-8) is thought to be the causative agent of kaposi's sarcoma, a vasoproliferative neoplasm, also commonly found in patients with aids. the presence of hhv-8 in a cohort of patients with bacillary angiomatosis was investigated.	1999	10748868
massive hemoperitoneum: a new manifestation of bacillary peliosis in human immunodeficiency virus infection.		1999	10825061
seroprevalence of bartonella henselae among hiv-1 infected patients in japan.		1999	10655688
[cat scratch disease].		1999	10635816
prevalence of bartonella henselae and bartonella clarridgeiae in an urban indonesian cat population.	we studied evidence of bartonella henselae and bartonella clarridgeiae infection in 54 cats living in jakarta, indonesia. by using an indirect immunofluorescence assay, we found immunoglobulin g antibody to b. henselae in 40 of 74 cats (54%). the blood of 14 feral cats was cultured on rabbit blood agar plates for 28 days. bartonella-like colonies were identified as b. henselae or b. clarridgeiae by using restriction fragment length polymorphism analysis and direct sequencing of the pcr amplicons ...	1999	9874661
images in clinical medicine. cat scratch disease.		1999	9887162
clinical and pathologic evaluation of chronic bartonella henselae or bartonella clarridgeiae infection in cats.	human bartonella infections result in diverse medical presentations, whereas many cats appear to tolerate chronic bacteremia without obvious clinical abnormalities. eighteen specific-pathogen-free cats were inoculated with bartonella henselae- and/or bartonella clarridgeiae-infected cat blood and monitored for 454 days. relapsing bacteremia did not correlate with changes in protein profiles or differences in antigenic protein recognition. intradermal skin testing did not induce a delayed type hy ...	1999	10203518
cat-scratch disease causing status epilepticus in children.	status epilepticus from cat-scratch encephalopathy is often recalcitrant to usual therapies, causing treatment to focus on critical care management of the patient that may require aggressive interventions, such as continuous pentobarbital administration. we describe two children whose initial clinical presentation of cat-scratch disease was status epilepticus with normal cerebrospinal fluid studies. a history of cat exposure (specifically, kitten and/or fleas), regional lymphadenopathy, and a pa ...	1999	9932833
bartonella henselae infection associated with peripapillary angioma, branch retinal artery occlusion, and severe vision loss.	to report atypical clinical features of bartonella henselae neuroretinitis treated with combination antibiotics.	1999	10030575
interactions of bartonella henselae with vascular endothelial cells.	the emerging human pathogen bartonella henselae has the remarkable capacity to colonise vascular tissues and to stimulate vasoproliferative tumour growth. although the molecular principle of bacterium-induced neovascularisation (angiogenesis) is still unclear, recent studies have indicated a novel mechanism of endothelial colonisation that involves the formation, engulfment and uptake of a large bacterial aggregate.	1999	10047560
bone pain after lymphadenitis. bartonella henselae granulomatous osteitis.		1999	10048617
cat-scratch disease with paravertebral mass and osteomyelitis.	the case of a 9-year-old girl with cat-scratch disease (csd) complicated by development of a paravertebral mass and osteomyelitis is presented. following multiple scratches and inguinal lymphadenopathy, she developed back pain, and imaging demonstrated a paravertebral mass with evidence of osteomyelitis involving vertebra t9. the diagnosis was made on the basis of detection of bartonella henselae by use of molecular techniques on an aspirate from the vertebral column and supportive serology for ...	1999	10064243
[cat-scratch disease: series of 14 cases. the diagnostic usefulness of serology].	disease by cat scratch (csd) is a syndrome characterized by regional adenopathies following cat scratch or bite. despite knowing the etiologic agent of the same, there is still controversy in the diagnosis of the syndrome in daily clinical practice.	1999	10069107
detection of bartonella henselae dna by two different pcr assays and determination of the genotypes of strains involved in histologically defined cat scratch disease.	cat scratch disease (csd) is a common cause of subacute regional lymphadenopathy, not only in children but also in adults. serological and molecular studies demonstrated that bartonella henselae is the etiologic agent in most cases of csd. amplification of b. henselae dna in affected tissue and detection of antibodies to b. henselae are the two mainstays in the laboratory diagnosis of csd. we designed a retrospective study and investigated formalin-fixed, paraffin-embedded lymph nodes from 60 pa ...	1999	10074515
bartonella koehlerae sp. nov., isolated from cats.	two of the 25 bartonella isolates recovered during a prevalence study of bartonella henselae bacteremia in domestic cats from the greater san francisco bay region were found to differ phenotypically and genotypically from all prior b. henselae isolates. these isolates, c-29 and c-30, which were recovered from the blood of two pet cats belonging to the same household, grew on chocolate agar as pinpoint colonies following 14 days of incubation at 35 degrees c in a candle jar but failed to grow on ...	1999	10074535
[prevalence of serum igg antibody against bartonella henselae in an asymptomatic japanese population].		1999	10077908
cat scratch disease diagnosed by polymerase chain reaction in a patient with suspected tuberculous lymphadenitis.	we present a patient diagnosed as having tuberculous lymphadenitis after lymph node biopsy and referred for tuberculous therapy. on review, because of recent cat exposure, she was tested for cat scratch disease (csd), but serological tests for bartonella henselae were negative. however, the diagnosis of csd was confirmed, and tuberculosis excluded, by polymerase chain reaction techniques. this case serves as a reminder that csd may mimic tuberculosis histologically and that sensitive molecular d ...	1999	10078182
[cat scratch disease].		1999	10088350
[bartonella infection in humans].	bartonella bacilliformis: among the 3 species of bartonella known to be human pathogens, b. bacilliformis causes carriun's disease, which manifests an acute phase (oroya fever) and a chronic phase marked by benign skin eruption with wart like macules of vascular origin. until 1993, b. bacilliformis was considered to be the only species in bartonella genus. in 1993, species formally in the rochalimaea genus were designated as bartonella species. bartonella quintana: this species causes trench fev ...	1999	10093604
prevalence of bartonella henselae immunoglobulin g antibodies in singaporean cats.	bartonella henselae causes several clinical diseases in humans. the most common infection is the classical cat-scratch disease (csd) occurring in immunocompetent and immunocompromised children exposed to newly acquired kittens. this is the first study of b. henselae infection in singaporean cat population.	1999	10093952
[three children with systemic cat scratch disease].	three girls with systemic cat scratch disease, aged 10, 13 and 9 years, were reported. they presented a prolonged fever and back pain in the early stage of the disease, and had no regional lymphadenopathy. two of them had hepatosplenic granulomas, one with multiple 5 mm hypoechoic lesions in the liver and spleen, and the other with a single 2.5 cm hypodense lesion in the left hepatic lobe. the latter patient underwent a partial left hepatic lobectomy. all patients had elevated titers of antibodi ...	1999	10222672
cat-scratch disease encephalopathy: a cause of status epilepticus in school-aged children.	we describe 6 school-aged patients who presented with status epilepticus (se) secondary to cat-scratch disease (csd) encephalopathy to alert clinicians to this distinctive clinical entity. the hospital database for admissions during 1 year was reviewed for patients presenting with se; 4 of 5 previously healthy school-aged children with se had csd encephalopathy based on elevated indirect fluorescent antibody titers to bartonella henselae. csd encephalopathy should be included in the differential ...	1999	10228301
serologic response to bartonella henselae in patients with cat scratch disease and in sick and healthy children.	indirect fluorescent antibody assay (ifa) is the most reliable test for detecting antibody to bartonella henselae in the diagnosis of cat scratch disease (csd). recently, an elisa test has been proposed, but conflicting results are reported. we compared igg-ifa and igg-igm elisa methods in csd patients and in healthy children. we also tested elisa specificity in a large group of healthy controls and in children with lymphoma-associated lymphadenopathy and with pyogenic lymphadenitis. the elisa p ...	1999	10229038
culture of bartonella quintana and bartonella henselae from human samples: a 5-year experience (1993 to 1998).	bartonella quintana and bartonella henselae are fastidious gram-negative bacteria responsible for bacillary angiomatosis, trench fever, cat scratch disease, and endocarditis. during a 5-year period, we received 2,043 samples for culture of bartonella sp. we found bartonella sp. to be the etiologic agent in 38 cases of endocarditis, 78 cases of cat scratch disease, 16 cases of bacteremia in homeless people, and 7 cases of bacillary angiomatosis. we correlated the results of positive cultures with ...	1999	10325344
acute clinical disease in cats following infection with a pathogenic strain of bartonella henselae (lsu16).	bartonella henselae is the causative agent of human cat scratch disease as well as several serious sequelae of infections, including bacillary angiomatosis and bacillary peliosis. conflicting reports describe the pathogenesis of b. henselae in the cat. in this study, we characterized a strain of b. henselae termed lsu16. this strain was isolated on rabbit blood agar from a naturally infected 10-month-old female cat during a recurrent episode of bacteremia. the bacterial species was confirmed by ...	1999	10338522
bartonella henselae and bartonella clarridgeiae infection in domestic cats from the philippines.	one hundred seven domestic cats from the philippines were serologically tested to establish the prevalence of bartonella infection. a subset of 31 of these cats also had whole blood collected to tentatively isolate bartonella strains. bartonella henselae and b. clarridgeiae were isolated from 19 (61%) of these cats. bartonella henselae type i was isolated from 17 (89%) of the 19 culture-positive cats. six cats (31%) were infected with b. clarridgeiae, of which four were coinfected with b. hensel ...	1999	10348234
a case of cat scratch disease masquerading as lymphogranuloma venereum.		1999	10361925