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omics approaches to study juvenile hormone synthesis.the juvenile hormones (jhs) are a family of insect acyclic sesquiterpenoids produced by the corpora allata (ca), a pair of endocrine glands connected to the brain. they are involved in the regulation of development, reproduction, behavior, caste determination, diapause, stress response, and numerous polyphenisms. in the post-genomics era, comprehensive analyses using functional 'omics' technologies such as transcriptomics, proteomics and metabolomics have increased our understanding of the activ ...201830551825
genetic variability of the aedes aegypti (diptera: culicidae) mosquito in el salvador, vector of dengue, yellow fever, chikungunya and zika.aedes aegypti is associated with dengue, yellow fever, chikungunya and zika viruses. this vector is widespread in tropical and subtropical areas, and can also occur in temperate areas at higher latitudes. the geographical distribution of ae. aegypti continues to spread due to human activities. this is the first study to examine the population genetic structure of this insect in el salvador, central america.201830547835
susceptible and mcry3a resistant corn rootworm larvae killed by a non-hemolytic bacillus thuringiensis cyt1aa mutant.the western corn rootworm (wcr) diabrotica virgifera virgifera causes substantial damage in corn. genetically modified (gm) plants expressing some bacillus thuringiensis (bt) insecticidal cry proteins efficiently controlled this pest. however, changes in wcr susceptibility to these bt traits have evolved and identification of insecticidal proteins with different modes of action against wcr is necessary. we show here for the first time that cyt1aa from bt exhibits toxicity against wcr besides to ...201830546034
aspergillus terreus (trichocomaceae): a natural, eco-friendly mycoinsecticide for control of malaria, filariasis, dengue vectors and its toxicity assessment against an aquatic model organism artemia nauplii.vector-borne diseases like malaria, filariasis, and dengue are transmitted by mosquitoes and they cause global mortality and morbidity due to an increased resistance against commercial insecticides. the present study was aimed to evaluate the neurobehavioral toxicity, knock-down effect, histopathology, ovicidal, adulticidal, and smoke toxicity effect of aspergillus terreus extract against three mosquito species, namely anopheles stephensi, culex quinquefasciatus, and aedes aegypti (diptera: culi ...201830534070
multispecies transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and aedes aegypti across the b. malayi life cycle.here, we present a comprehensive transcriptomics data set of brugia malayi, its wolbachia endosymbiont wbm, and its vector host. this study samples from 16 stages across the entire b. malayi life cycle, including stage 1 through 4 larvae, adult males and females, embryos, immature microfilariae, and mature microfilariae.201830533772
comparison of landmark- and outline-based geometric morphometrics for discriminating mosquito vectors in ratchaburi province, thailand.it is often challenging to identify mosquito vectors in the field based on morphological features due to their similar morphologies and difficulties in obtaining undamaged samples but is required for their successful control. geometric morphometrics (gm) overcomes this issue by analyzing a suite of traits simultaneously and has the added advantages of being easy to use, low cost, and quick. therefore, this research compared the efficiency and precision of landmark- and outline-based gm technique ...201830533435
dengue outbreaks in hawai'i after wwii - a review of public health response and scientific literature.the four serotypes of dengue virus (denv) cause the most important and common arthropod-borne viral diseases in humans. there have been three major dengue outbreaks in hawai'i since 1946. the most recent and largest outbreak occurred on hawai'i island in 2015-2016. this article reviews the public health response to dengue outbreaks over the period 2001-2016, as well as scientific literature on dengue outbreaks in hawai'i. as summarized in the assessment by the centers for disease control and pre ...201830533283
correction: mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.[this corrects the article doi: 10.1371/journal.pone.0193164.].201830532207
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201830532189
soluble expression of recombinant midgut zymogen (native propeptide) proteases from the aedes aegypti mosquito utilizing e. coli as a host.studying proteins and enzymes involved in important biological processes in the aedes aegypti mosquito is limited by the quantity that can be directly isolated from the mosquito. adding to this difficulty, digestive enzymes (midgut proteases) involved in metabolizing blood meal proteins require a more oxidizing environment to allow proper folding of disulfide bonds. therefore, recombinant techniques to express foreign proteins in escherichia coli prove to be effective in producing milligram quan ...201830563449
simultaneous detection of different zika virus lineages via molecular computation in a point-of-care assay.we have developed a generalizable "smart molecular diagnostic" capable of accurate point-of-care (poc) detection of variable nucleic acid targets. our isothermal assay relies on multiplex execution of four loop-mediated isothermal amplification reactions, with primers that are degenerate and redundant, thereby increasing the breadth of targets while reducing the probability of amplification failure. an easy-to-read visual answer is computed directly by a multi-input boolean or logic gate (gate o ...201830558136
larval exposure to the bacterial insecticide bti enhances dengue virus susceptibility of adult aedes aegypti mosquitoes.understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. in medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. we addressed this hypothesis in the mosquito aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (denv) and the chi ...201830558130
evolution of maternal and zygotic mrna complements in the early drosophila embryo.the earliest stages of animal development are controlled by maternally deposited mrna transcripts and proteins. once the zygote is able to transcribe its own genome, maternal transcripts are degraded, in a tightly regulated process known as the maternal to zygotic transition (mzt). while this process has been well-studied within model species, we have little knowledge of how the pools of maternal and zygotic transcripts evolve. to characterize the evolutionary dynamics and functional constraints ...201830557299
aedes aegypti galectin competes with cry11aa for binding to alp1 to modulate cry toxicity.the key step for the toxicity of bacillus thuringiensis subsp. israelensis (bti) is the interaction between toxins and putative receptors; thus, many studies focus on identification of new toxin receptors and engineering of toxins with higher affinity/specificity for receptors. in the larvae of aedes aegypti, galectin-14 was one of the genes upregulated by bti treatment. rnai knockdown expression of galectin-14 and feeding recombinant galectin-14-thioredoxin fusion protein significantly affected ...201830556692
evaluation of the leaf essential oil from artemisia vulgaris and its larvicidal and repellent activity against dengue fever vector aedes aegypti-an experimental and molecular docking investigation.aedes aegypti is a mosquito vector that spreads dengue fever and yellow fever worldwide in tropical and subtropical countries. essential oil isolated from artemisia vulgaris is found to have larvicidal and repellent action against this vector. the dried leaves were subjected to hydrodistillation using a clevenger-type apparatus for 4 h. the isolated essential oil was analyzed by using gas chromatography-mass spectrometry, and the major insecticidal compounds were identified as α-humulene (0.72%) ...201830556010
zika in puerto rico, 2016-2017: ii perspectives on epidemic surveillance and control, health communication, outcomes and lessons.the social reaction to the zika epidemic in puerto rico reached a confrontational climax regarding aerial fumigation with an organophosphate insecticide. the public drama has obscured multiple simultaneous controversies. this and a companion paper, based mostly on print and digital news reports, provide a context and description of the major controversies and examine the outcomes and their lessons for the protection of the public's health. part ii covers the questions on disease surveillance (wh ...201830576574
zika virus in the americas: an environmental health perspective.phylogenetic studies suggest that zikv may have been introduced to brazil, and therefore to the americas, in 2014 during the world spring canoe championship held in the city of rio de janeiro. since then the virus has spread across latin america, caribbean, and north america. it seems clear that aedes aegypti and, to a lesser extent, aedes albopictus are the main vectors of the pathogen. zikv infection symptoms are similar to other flaviviruses such as a dengue infection and therefore can be eas ...201830576572
the pagodas protocol: pediatric assessment group of dengue and aedes saliva protocol to investigate vector-borne determinants of aedes-transmitted arboviral infections in cambodia.mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in southeast asia. when the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alter the immune response and influence host receptivity to infection, and consequently, the establishment of the virus. previous reports have highlighted the complexity of mosquito vector-derive ...201830572920
construction sites in miami-dade county, florida are highly favorable environments for vector mosquitoes.urbanization is increasing globally, and construction sites are an integral part of the urbanization process. it is unknown to what extent construction sites create favorable breeding conditions for mosquitoes. the main objectives of the present study were to identify what species of mosquitoes are present at construction sites and the respective physical features associated with their production. eleven construction sites were cross-sectionally surveyed for the presence of mosquitoes in miami-d ...201830571764
dengue outbreaks and the geographic distribution of dengue vectors in taiwan: a 20-year epidemiological analysis.dengue fever is an important mosquito-borne viral infectious disease that mostly occurs in tropical and subtropical areas of the world. according to epidemiological data from the center for disease control of taiwan, more than 98.62% of outbreaks of indigenous total dengue cases were reported in the southern part of taiwan. southern taiwan is an aggregate area encompassing tainan, kaohsiung, and pingtung, all of which are located below the tropic of cancer (23º35'n). with a few exceptions, dengu ...201830580791
dengue in taiwan: pointing the finger at aedes aegypti.in this issue of the biomedical journal we explore the history of dengue infection in taiwan and what current trends have to say about the vector responsible for transmitting the disease on the island. we focus on original research reporting the development of a new perfusion bioreactor to engineer bone from human cord blood stem cells. finally, we look at trends in osteoporosis in taiwan and how they highlight the success of public health campaigns.201830580790
long-term exposure of aedes aegypti to bacillus thuringiensis svar. israelensis did not involve altered susceptibility to this microbial larvicide or to other control agents.bacillus thuringiensis svar. israelensis (bti) is an effective and safe biolarvicide to control aedes aegypti. its mode of action based on four protoxins disfavors resistance; however, control in endemic areas that display high mosquito infestation throughout the year requires continuous larvicide applications, which imposes a strong selection pressure. therefore, this study aimed to investigate the effects of an intensive bti exposure on an ae. aegypti strain (recbti), regarding its susceptibil ...201830594214
biochemical effects of petroselinum crispum (umbellifereae) essential oil on the pyrethroid resistant strains of aedes aegypti (diptera: culicidae).in ongoing screening research for edible plants, petroselinum crispum essential oil was considered as a potential bioinsecticide with proven antimosquito activity against both the pyrethroid susceptible and resistant strains of aedes aegypti. due to the comparative mosquitocidal efficacy on these mosquitoes, this plant essential oil is promoted as an attractive candidate for further study in monitoring resistance of mosquito vectors. therefore, the aim of this study was to evaluate the impact of ...201830586929
a comparative study of energy contents in mosquito vectors of malaria and dengue prevailing in jodhpur city (thar desert) of rajasthan state, india.transmission of malaria and dengue in the desert part of india is mainly caused by anopheles stephensi and aedes aegypti respectively. the maintenance and transmission of the pathogens that cause malaria and dengue are dependent on the physiology of the mosquito vectors. we aimed to measure the energy contents in the mosquitoes transmitting malaria and dengue in the desert part of the country.201830584552
combined sterile insect technique and incompatible insect technique: sex separation and quality of sterile aedes aegypti male mosquitoes released in a pilot population suppression trial in thailand.the sterile insect technique (sit), which is based on irradiation-induced sterility, and incompatible insect technique (iit), which is based on wolbachia-induced cytoplasmic incompatibility (a kind of male sterility), have been used as alternative methods to reduce mosquito vector populations. both methods require the release of males to reduce fertile females and suppress the number of natural populations. different techniques of sex separation to obtain only males have been investigated previo ...201830583749
molecular tools to create new strains for mosquito sexing and vector control.vector control programs based on population reduction by matings with mass-released sterile insects require the release of only male mosquitoes, as the release of females, even if sterile, would increase the number of biting and potentially disease-transmitting individuals. while small-scale releases demonstrated the applicability of sterile males releases to control the yellow fever mosquito aedes aegypti, large-scale programs for mosquitoes are currently prevented by the lack of efficient sexi ...201830583736
functional analysis of the promoter of an early zygotic gene klc2 in aedes aegypti.aedes aegypti is an important mosquito vector that transmits arboviruses that cause devastating diseases including zika, dengue fever, yellow fever and chikungunya. improved understanding of gene regulation in the early development of ae. aegypti will facilitate genetic studies and help the development of novel control strategies of this important disease vector.201830583735
sex determination and aedes population control.the global economic cost of aedes-borne diseases, such as dengue, is estimated to be in the billions of dollars annually. in this scenario, a sustained vector control strategy is the only alternative to control dengue, as well as other diseases transmitted by aedes, including zika and chikungunya. the use of transgenic mosquitoes is a promising weapon in the improvement of approaches currently applied in aedes aegypti control. field trials using genetically modified mosquitoes for population con ...201830583731
pure early zygotic genes in the asian malaria mosquito anopheles stephensi.the asian malaria mosquito, anopheles stephensi, is a major urban malaria vector in the middle east and on the indian subcontinent. early zygotic transcription, which marks the maternal-to-zygotic transition, has not been systematically studied in an. stephensi or any other anopheles mosquitoes. improved understanding of early embryonic gene expression in an. stephensi will facilitate genetic and evolutionary studies and help with the development of novel control strategies for this important di ...201830583723
exploring the potential of computer vision analysis of pupae size dimorphism for adaptive sex sorting systems of various vector mosquito species.several mosquito population suppression strategies based on the rearing and release of sterile males have provided promising results. however, the lack of an efficient male selection method has hampered the expansion of these approaches into large-scale operational programmes. currently, most of these programmes targeting aedes mosquitoes rely on sorting methods based on the sexual size dimorphism (ssd) at the pupal stage. the currently available sorting methods have not been developed based on ...201830583722
current status of aedes aegypti insecticide resistance development from banjarmasin, kalimantan, indonesia.aedes aegypti represents the principal vector of many arthropod-borne diseases in tropical areas worldwide. since mosquito control strategies are mainly based on use of insecticides, resistance development can be expected to occur in frequently exposed ae. aegypti populations. surveillance on resistance development as well as testing of insecticide susceptibility is therefore mandatory and needs further attention by national/international public health authorities. in accordance, we here conduct ...201830671445
zebrafish embryo toxicity of a binary mixture of pyrethroid insecticides: d-tetramethrin and cyphenothrin.pesguard fg161™, a mixture of d-tetramethrin and cyphenothrin (1:3 ratio), is extensively used to achieve rapid control of adult dengue vector, aedes aegypti, during the disease outbreaks. both d-tetramethrin and cyphenothrin are synthetic pyrethroids that are known to have adverse effects on non-mammalian organisms such as fish. the present study intended to use zebrafish embryo toxicity model to investigate the toxic effect of the above binary mixture on fish. particularly, zebrafish embryo to ...201830687404
insecticidal and repellent properties of novel trifluoromethylphenyl amides ii.this project focused on the design, synthesis, and testing of trifluoromethylphenyl amides (tfmpas) as potential mosquitocides and repellents. fourteen compounds were evaluated for toxicity against larvae and adults of aedes aegypti. several compounds were toxic against aedes aegypti (larval, adult and feeding bioassays) and drosophila melanogaster (glass-surface contact assay), but were much less toxic than fipronil, with toxicity ratios ranging from 100-fold in the larval assay to 100,000-fold ...201830704711
toxicity, mode of action, and synergist potential of flonicamid against mosquitoes.the present study focused on the toxicity of the aphid anti-feedant flonicamid and its main metabolite, 4-trifluoromethylnicotinamide (tfna-am) to aedes aegypti and anopheles gambiae mosquitoes. the compounds were toxic to both species via topical application, resulting in un-coordinated locomotion and leg splaying, with a favorable an. gambiae ld50 value of 35 ng/mg for tfna-am, but no significant lethality to ae. aegypti at 10 μg/female. there was mild cross resistance in the akron-kdr (akdr) ...201830704710
use of chemical probes to explore the toxicological potential of the k+/cl- cotransporter (kcc) as a novel insecticide target to control the primary vector of dengue and zika virus, aedes aegypti.the majority of commercialized insecticides target the insect nervous system and therefore, neural proteins are well-validated targets for insecticide development. considering that only a few neural targets are exploited for insecticidal action and the development of insecticide resistance has reduced the efficacy of current insecticidal classes, we sought to test the toxicological potential of the potassium-chloride cotransporter (kcc). in mammals, kcc proteins have seminal roles in shaping gab ...201830704707
wing geometry analysis of aedes aegypti (diptera, culicidae), a dengue virus vector, from multiple geographical locations of samut songkhram, thailand.dengue haemorrhagic fever (dhf) is a mosquito-borne disease and remains a major public health problem, especially in tropical and temperate countries. studying wing morphometric of aedes aegypti as a mosquito vector of dhf can help to better understand biological process of the mosquito adaptation to the environment. we aimed to study the geometric morphometric of ae. aegypti from multiple geographical areas.201830918904
larvicidal potential of five selected dragonfly nymphs in sri lanka over aedes aegypti (linnaeus) larvae under laboratory settings.limitations in breeding source reduction practices, development of insecticide resistance in mosquitoes, and ill effects of chemical controlling methods on human and ecosystem health have motivated sri lankan authorities working for dengue control to seek for alternative, ecofriendly, and sustainable approaches for controlling of aedes vectors, to manage dengue epidemics. the present study attempted to investigate the predation efficiency of locally available dragonfly nymphs over aedes aegypti ...201830627580
mapping the global potential distributions of two arboviral vectors aedes aegypti and ae. albopictus under changing climate.aedes aegypti and ae. albopictus are the primary vectors that transmit several arboviral diseases, including dengue, chikungunya, and zika. the world is presently experiencing a series of outbreaks of these diseases, so, we still require to better understand the current distributions and possible future shifts of their vectors for successful surveillance and control programs. few studies assessed the influences of climate change on the spatial distributional patterns and abundance of these impor ...201830596764
zika virus infection produces a reduction on aedes aegypti lifespan but no effects on mosquito fecundity and oviposition success.a zika virus (zikv) pandemic started soon after the first autochthonous cases in latin america. although aedes aegypti is pointed as the primary vector in latin america, little is known about the fitness cost due to zikv infection. we investigated the effects of zikv infection on the life-history traits of ae. aegypti females collected in three districts of rio de janeiro, brazil (barra, deodoro, and porto), equidistant ~25 km each other. aedes aegypti mosquitoes were classified into infected (a ...201830619118
geographical distribution of aedes aegypti and aedes albopictus (diptera: culicidae) and genetic diversity of invading population of ae. albopictus in the republic of the congo.background: the arbovirus vector, aedes albopictus, originating from asia, has recently invaded african countries, including the republic of the congo, where it was associated with a chikungunya outbreak. up until now, little was known about its distribution in relation to the native aedes aegypti and how the invasion will modify the epidemiology of arboviral diseases. here, we assessed the current distribution of ae. albopictus and ae. aegypti in the republic of the congo and explored the genet ...201830175244
estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever outbreaks.in this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ...201730137722
fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures.bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.201729664029
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
ultrasound is not useful as a screening tool for dengue fever.dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ...201729657635
infectivity of chordodes nobilii larvae (gordiida: nematomorpha).the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ...201729465953
environmental factors can influence dengue reported cases.global climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo.201729451659
[clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico].describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital.201731391823
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
[(assessment of risk for arbovirus infections in russia)].the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ...201730721608
larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae).acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ...201729644817
emergent acoustic order in arrays of mosquitoes.the yellow fever mosquito aedes aegypti forms aerial swarms that serve as mating aggregations [1]. despite lacking the remarkable collective order of other animal ensembles, such as fish and birds [2], the kinematic properties of these swarms bear the hallmarks of local interaction and global cohesion [3,4]. however, the mechanisms responsible for collective behaviour in mosquitoes are not well understood. mosquitoes use their antennae as hearing organs to locate and interact with one another vi ...201729161555
dengue virus detection in aedes aegypti larvae and pupae collected in rural areas of anapoima, cundinamarca, colombia.there is a high incidence and prevalence of dengue in the department of cundinamarca, and recently aedes aegypti, the main vector of dengue virus (denv), was detected in some of its rural areas.201729161491
effect of the type and number of washes in the efficacy of long lasting insecticidal nets for aedes aegypti control in girardot, colombia.the use of long lasting insecticidal materials has shown promising results in the control of aedes aegypti.201729161489
virological surveillance of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus as support for decision making for dengue control in medellín.dengue represents an important public health problem in colombia. no treatment is available and the vaccine has not been approved in all countries, hence, actions should be strengthened to mitigate its impact through the control of aedes aegypti, the vector mosquito. in colombia, surveillance is done using entomological indexes and case notification, which is usually informed late, leading to untimely interventions. viral detection in urban mosquitoes using molecular techniques provides more acc ...201729161487
aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia.aedes aegypti and ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and zika arboviruses in several countries worldwide. in colombia, ae. albopictus geographical distribution has increased to include highly populated cities such as cali and medellín. although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known.201729161485
[laboratory susceptibility tests of aedes aegypti and culex quinquefasciatus larvae to the entomopathogenic nematode heterorhabditis bacteriophora].aedes aegypti is the vector of dengue, yellow fever, zika and chikungunya viruses, and culex quinquefasciatus is the vector of st. louis and west nile encephalitis viruses.201729161479
[spatial distribution of potential and positive aedes aegypti breeding sites].the spatial distribution of aedes aegypti is heterogeneous, and the interaction between positive and potential breeding sites located both inside and outside homes is one of the most difficult aspects to characterize in vector control programs.201729161478
[spatial distribution of aedes aegypti (diptera: culicidae) in the rural area of two municipalities of cundinamarca, colombia].it is difficult to adapt or create new strategies for dengue control in rural areas due to the dispersion of homes and the lack of knowledge about the presence and behavior of the vector in these areas. spatial data analysis could help understand time and space dynamics related to the transmission of vector-borne diseases in rural areas and, thus, contribute to the prevention and control of dengue.201729161476
alternative control of aedes aegypti resistant to pyrethroids: lethal and sublethal effects of monoterpenes bioinsecticides.the mosquito aedes aegypti is intensely controlled because it is vector of viruses that cause innumerous diseases, especially in tropical regions. due to the indiscriminate use of insecticides, populations from different regions have been resistant to pyrethroids. here, we analyzed the lethal and sublethal effects of essential oil of aristolochia trilobata and its major compounds on a. aegypti from susceptible and pyrethroid resistant populations.201729160036
upregulation of aedes aegypti vago1 by wolbachia and its effect on dengue virus replication.dengue infection along with its related disease conditions poses a significant threat to human health. the pathogen responsible for this infection is dengue virus (denv) which is primarily transmitted to humans through the bites of aedes aegypti mosquitoes. unavailability of a potent vaccine has recently sparked renewed research endeavours aimed at vector control. to date, wolbachia as an endosymbiotic bacterium has shown promise as a novel biocontrol agent to restrict denv replication in the ve ...201729157676
a call to introduce structured zika surveillance in india.india has the climatic conditions conducive to year-round transmission of zika virus, and a structured disease surveillance program should be implemented to prevent an outbreak. such a program should (i) start screening before an outbreak arises; (ii) collect baseline data to assess future disease risk and monitor potential birth defects; and (iii) provide new insights into the ecology of the disease and inform public health policy following the one health concept.201729153262
effective population sizes of a major vector of human diseases, aedes aegypti.the effective population size (ne ) is a fundamental parameter in population genetics that determines the relative strength of selection and random genetic drift, the effect of migration, levels of inbreeding, and linkage disequilibrium. in many cases where it has been estimated in animals, ne is on the order of 10%-20% of the census size. in this study, we use 12 microsatellite markers and 14,888 single nucleotide polymorphisms (snps) to empirically estimate ne in aedes aegypti, the major vecto ...201729151858
vectors, hosts, and control measures for zika virus in the americas.we examine zika virus (zikv) from an ecological perspective and with a focus on the americas. we assess (1) the role of wildlife in zikv disease ecology, (2) how mosquito behavior and biology influence disease dynamics, and (3) how nontarget species and ecosystems may be impacted by vector control programs. our review suggests that free-ranging, non-human primates may be involved in zikv transmission in the old world; however, other wildlife species likely play a limited role in maintaining or t ...201729150828
the introduction of dengue follows transportation infrastructure changes in the state of acre, brazil: a network-based analysis.human mobility, presence and passive transportation of aedes aegypti mosquito, and environmental characteristics are a group of factors which contribute to the success of dengue spread and establishment. to understand this process, we assess data from dengue national and municipal basins regarding population and demographics, transportation network, human mobility, and ae. aegypti monitoring for the brazilian state of acre since the first recorded dengue case in the year 2000 to the year 2015. d ...201729149175
baccharis reticularia dc. and limonene nanoemulsions: promising larvicidal agents for aedes aegypti (diptera: culicidae) control.baccharis reticularia dc. is a plant species from the asteraceae family that is endemic to brazil. despite the great importance of baccharis genus, no study has been carried out regarding either the phytochemical composition of b. reticularia or the evaluation of its larvicidal potential. considering the intrinsic immiscibility of essential oils, this study shows larvicidal nanoemulsions containing the b. reticularia phytochemically characterized essential oil and its main constituent against ae ...201729149027
[dengue fever : symptoms, epidemiology, entomology, pathogen diagnosis and prevention].dengue infections are among the most frequent causes of febrile disease in tropical climates. infections are caused by a flavivirus transmitted by the aedes mosquito. aedes aegypti mosquitos are the main transmitters of dengue viruses. since these insects are both diurnal and container breeders, particular prevention and control measures are required. symptom severity varies and can range from a mild, flu-like clinical picture to severe hemorrhage and shock. the most common symptoms experienced ...201729147722
development and physiological effects of an artificial diet for wolbachia-infected aedes aegypti.the endosymbiotic bacterium wolbachia spreads rapidly through populations of aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and zika viruses. mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there is a dearth of mass rearing infrastructure specific to wolbachia-infected mosquitoes. one example is the lack of a blood meal substitute, which accounts for the wolbachia-specific ph ...201729146940
behavioral, climatic, and environmental risk factors for zika and chikungunya virus infections in rio de janeiro, brazil, 2015-16.the burden of arboviruses in the americas is high and may result in long-term sequelae with infants disabled by zika virus infection (zikv) and arthritis caused by infection with chikungunya virus (chikv). we aimed to identify environmental drivers of arbovirus epidemics to predict where the next epidemics will occur and prioritize municipalities for vector control and eventual vaccination. we screened sera and urine samples (n = 10,459) from residents of 48 municipalities in the state of rio de ...201729145452
zika virus: an updated review of competent or naturally infected mosquitoes.zika virus (zikv) is an arthropod-borne virus (arbovirus) that recently caused outbreaks in the americas. over the past 60 years, this virus has been observed circulating among african, asian, and pacific island populations, but little attention has been paid by the scientific community until the discovery that large-scale urban zikv outbreaks were associated with neurological complications such as microcephaly and several other neurological malformations in fetuses and newborns. this paper is a ...201729145400
shifting the paradigm in dirofilaria immitis prevention: blocking transmission from mosquitoes to dogs using repellents/insecticides and macrocyclic lactone prevention as part of a multimodal approach.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra® 3d, ceva animal health) combined with a macrocyclic lactone (milbemycin oxime, mbo, interceptor®, virbac) on transmission of heartworm l3 from mosquitoes to dogs and subsequent development of worms in treated dogs exposed to infected mosquitoes.201729143678
blocking the transmission of heartworm (dirofilaria immitis) to mosquitoes (aedes aegypti) by weekly exposure for one month to microfilaremic dogs treated once topically with dinotefuran-permethrin-pyriproxyfen.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra®3d, ceva animal health) on the acquisition of heartworm microfilariae by mosquitoes exposed to microfilaremic dogs weekly for 1 month.201729143651
corrigendum: rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.[this corrects the article on p. 283 in vol. 8, pmid: 28536536.].201729142533
the importance of human population characteristics in modeling aedes aegypti distributions and assessing risk of mosquito-borne infectious diseases.the mosquito aedes aegypti has long been a vector for human illness in the southeastern united states. in the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the zika virus that has been introduced to the region. multiple studies have modeled the geographic distribution of ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. furthermore, ae. aegypti thrives in areas where ...201729167627
determination of the efficiency of diets for larval development in mass rearing aedes aegypti (diptera: culicidae).larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. therefore, the current study was carried out to optimize the larval diet for mass rearing of aedes aegypti, for sterile insect technique (sit)-based applications in sri lanka. five batches of 750 first instar larvae (l 1) of ae. aegypti were exposed to five different concentrations (2-10%) of international atomic energy agency (iaea) recommended the larval diet. morphological development parameters ...201729166980
biology of two larval morphological phenotypes of aedes aegypti in abidjan, côte d'ivoire.since 2008, several outbreaks of yellow fever and dengue occurred in abidjan, the economic capital of côte d'ivoire. a better knowledge of the biology of aedes aegypti populations, the main vector of yellow fever and dengue viruses, is necessary to tailor vector control strategies implemented in the city. this study was designed to determine some biological parameters, occurring during the life cycle of two morphological phenotypes of ae. aegypti larvae. mosquitoes were sampled in a suburb of ab ...201729166962
feeding-induced changes in allatostatin-a and short neuropeptide f in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, aedes aegypti.aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. sugar feeding and host seeking in female a. aegypti are transiently inhibited following a blood meal. this inhibition is partially mediated by short neuropeptide f (snpf). the paired antennal lobes (als), as the first processing centers for olfactory information, has been shown to play a significant role in the neuropeptidergic regulation of odor-mediated behavi ...201729166650
wolbachia infection alters the relative abundance of resident bacteria in adult aedes aegypti mosquitoes, but not larvae.insect-symbiont interactions are known to play key roles in host functions and fitness. the common insect endosymbiont wolbachia can reduce the ability of several human pathogens, including arboviruses and the malaria parasite, to replicate in insect hosts. wolbachia does not naturally infect aedes aegypti, the primary vector of dengue virus, but transinfected ae. aegypti have antidengue virus properties and are currently being trialled as a dengue biocontrol strategy. here, we assess the impact ...201729165845
insecticide susceptibility status in mexican populations of stegomyia aegypti (= aedes aegypti): a nationwide assessment.in mexico, mosquito vector-borne diseases are of public health concern as a result of their impact on human morbidity and mortality. the use of insecticides against adult mosquitoes is one of the most common ways of controlling mosquito population densities. however, the use of these compounds has resulted in the development of insecticide resistance. the aim of this study was to estimate susceptibility to six pyrethroids, two carbamates and two organophosphates in mexican populations of stegomy ...201729165810
larvicidal, histopathological efficacy of penicillium daleae against larvae of culex quinquefasciatus and aedes aegypti plus biotoxicity on artemia nauplii a non-target aquatic organism.mosquitoes can transmit the terrible diseases to human beings. soil-borne fungal products act as potential source for low-cost chemicals, used for developing eco-friendly control agents against mosquito-vector borne diseases. the prime aim of study was to check the larvicidal potential of fungus mycelia (by ethyl acetate solvent) extract from penicillium daleae (kx387370) against culex quinquefasciatus and aedes aegypti and to test the toxicity of brine shrimp artemia nauplii, by observing the p ...201729163159
involvement of cecropin b in the formation of the aedes aegypti mosquito cuticle.in this study, we found a mosquito antimicrobial peptide (amp), aedes aegypti cecropin b (aacec b), was expressed constitutively in pupae. knockdown in the pupae of aacec b using double-stranded rna (dsrna) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. simultaneous injection of aacec b dsrna and aacec b peptide into pupa ...201729180688
a peptide signaling system that rapidly enforces paternity in the aedes aegypti mosquito.female aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. this single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. ae. aegypti mate in flight near human hosts [6], and females b ...201729174895
aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication.arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ...201729247247
zika virus can be venereally transmitted between aedes aegypti mosquitoes.alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions.201729246237
persistence of toxic activity of fermentation extracts from bacillus thuringiensis var. israelensis after more than three decades of storage.this study was carried out to determine the persistence of toxicity of fermentation extracts of bacillus thuringiensis var. israelensis after more than three decades of storage. for this purpose, a population of aedes aegypti was established. the mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified who protocol. the extracts with the highest mortality rate were determined in triplicat ...201729358951
analytical report of the 2016 dengue outbreak in córdoba city, argentina.after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...201729239555
assessing natural infection with zika virus in the southern house mosquito, culex quinquefasciatus, during 2016 in puerto rico.the epidemic of zika in the western hemisphere has led to intense investigations of all species important in the transmission of zika virus (zikv), including putative mosquito vectors. although evidence points to stegomyia (= aedes) (diptera: culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. herein, field-caught culex quinquefasciatus (diptera: culicidae) collec ...201729239003
aedes aegypti breeding site in an underground rainwater reservoir: a warning.we describe the discovery of aedes aegypti underground breeding site in the pinheiros neighborhood of são paulo, sp, during an entomological survey program performed in 2016. even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. a detailed investigation allowed for the detection of ae. aegypti in an underground reservoir used for rainwater storage. after the implementation of protection screens in the accesses, the presence of the vector was no ...201729236877
detection of zika virus in aedes mosquitoes from mexico.we report on the results of an entomovirological surveillance system of aedes populations performed by the ministry of health of the central state of san luis potosí, mexico.201729232453
efficacy of 13 commercial household aerosol insecticides against aedes aegypti (diptera: culicidae) from morelos, mexico.in mexico, aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue, zika, and chikungunya viruses. control programs include community participation using personal protection such as household aerosol insecticides. in both, urban or rural areas, the use of aerosol insecticides is a common practice to avoiding mosquito biting. thus, information on the efficacy of commercial products must be available. this study reports the efficacy of 13 household aerosol insecticides against ae. ...201729228197
the first detected airline introductions of yellow fever mosquitoes (aedes aegypti) to europe, at schiphol international airport, the netherlands.air-borne introduction of exotic mosquitoes to schiphol airport in the netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. beginning in 2013, surveillance efforts at schiphol had focused on promptly detecting accidental introductions at the airport facilities in order to quickly react and avoid temporary proliferation or establishment of mosquito populations, identify the origin of the introductions, and avoid potential trans ...201729221490
full-length genome sequence of a chikungunya virus isolate from the 2017 autochthonous outbreak, lazio region, italy.we report here the genome sequence of a human chikungunya virus isolate from the ongoing autochthonous outbreak in central italy. the sequence (east-central-south african lineage, indian ocean sublineage), which is similar to recent sequences from pakistan and india, shows e1 and e2 signatures of strains whose main mosquito vector is aedes aegypti, although aedes albopictus is the vector in italy.201729217791
the gastric caecum of larval aedes aegypti: stimulation of epithelial ion transport by 5-hydroxytryptamine and camp.we report measurements of ion transport across the gastric caecum of larvae of aedes aegypti, a vector of yellow fever that inhabits a variety of aquatic habitats ranging from freshwater to brackish water. we provide the first measurements of the effect of 5-hydroxytryptamine (5-ht) on transepithelial potential (tep), luminal ion concentrations and electrochemical potentials, as well as basolateral membrane potential and h+, na+ and k+ fluxes. tep, basolateral membrane potential, and h+, k+, and ...201729217627
the immune strategies of mosquito aedes aegypti against microbial infection.yellow fever mosquito aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, chikungunya virus, and zika virus, which cause great concern to human health. mosquito control is an effective method to block the spread of infectious diseases. ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. in this review, we briefly summarize the recent findings in the immune response of ae. aegypti again ...201729217264
differences in prevalence of symptomatic zika virus infection by age and sex-puerto rico, 2016.during the zika virus (zikv) outbreak in puerto rico in 2016, non-pregnant women aged 20-39 years were disproportionately identified with zikv disease. we used household-based cluster investigations to determine if this disparity was associated with age- or sex-dependent differences in the rate of zikv infection or reporting symptoms.201729216376
novel wolbachia-transinfected aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.wolbachia pipientis from drosophila melanogaster (wmel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, zika, and chikungunya viruses, when introduced into the mosquito vector aedes aegypti. to date, wmel-infected ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. despite the success in establishing wmel-infected mosquitoes in wild populati ...201729216317
dengue serotype-specific immune response in aedes aegypti and aedes albopictus.dengue viruses (denv) are considered one of the most important emerging pathogens and dengue disease is a global health threat. the geographic expansion of dengue viruses has led to co-circulation of all four dengue serotypes making it imperative that new denv control strategies be devised.201729211244
an aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity.transmission of dengue virus (denv) requires successful completion of the infection cycle in the aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. while certain ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to denv infection. we show that a talaromyces (tsp_pr) fungus, isolated from field-caught ae. aegypti, ...201729205153
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