Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| three-dimensional structure of the cytoskeleton in trichomonas vaginalis revealed new features. | the flagellated protozoan trichomonas vaginalis has been widely studied owing to its medical significance and unique structure. the complicated three-dimensional (3d) structure of the cellular components of t. vaginalis was reconstructed from serial sections to enable observation of the spatial features of the whole cell. electron tomography was used to examine the detailed structure of the cellular organelles. tomographic reconstruction showed the mastigont system and the parabasal filament of ... | 2009 | 19386993 |
| simple and inexpensive point-of-care tests improve diagnosis of vaginal infections in resource constrained settings. | bacterial vaginosis (bv) and trichomonas vaginalis infection (tv) have been associated with adverse birth outcomes and increased risk for hiv. we compare the performance of simple inexpensive point-of-care (poc) tests to laboratory diagnosis and syndromic management of bv and tv in poor settings. | 2009 | 19392745 |
| alfred francois donné (1801-78): a pioneer of microscopy, microbiology and haematology. | alfred françois donné is widely known in the scientific community as the discoverer of trichomonas vaginalis, since he was the first to illustrate the parasite that later was recognized to cause vaginal infections. however, his other, less-known findings are equally important: he was also the inventor of the photoelectric microscope, with the assistance of his student léon foucault, as well as the first to apply photography to microscopic preparations (daguerreotype). his research in microscopy ... | 2009 | 19401511 |
| characteristics of hiv-1 discordant couples enrolled in a trial of hsv-2 suppression to reduce hiv-1 transmission: the partners study. | the partners hsv-2/hiv-1 transmission study (partners study) is a phase iii, placebo-controlled trial of daily acyclovir for genital herpes (hsv-2) suppression among hiv-1/hsv-2 co-infected persons to reduce hiv-1 transmission to their hiv-1 susceptible partners, which requires recruitment of hiv-1 serodiscordant heterosexual couples. we describe the baseline characteristics of this cohort. | 2009 | 19404392 |
| use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. | to evaluate a new dual priming oligonucleotide (dpo)-based multiplex polymerase chain reaction (pcr) assay for detection of six sexually transmitted pathogens, including chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum and trichomonas vaginalis. | 2009 | 19413770 |
| trichomonas vaginalis: metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. implications for nitroimidazole toxicity and resistance. | infections with the microaerophilic parasite trichomonas vaginalis are treated with the 5-nitroimidazole drug metronidazole, which is also in use against entamoeba histolytica, giardia intestinalis and microaerophilic/anaerobic bacteria. here we report that in t. vaginalis the flavin enzyme thioredoxin reductase displays nitroreductase activity with nitroimidazoles, including metronidazole, and with the nitrofuran drug furazolidone. reactive metabolites of metronidazole and other nitroimidazoles ... | 2009 | 19415801 |
| current and future perspectives on the chemotherapy of the parasitic protozoa trichomonas vaginalis and entamoeba histolytica. | trichomonas vaginalis and entamoeba histolytica are clinically important protozoa that affect humans. t. vaginalis produces sexually transmitted infections and e. histolytica is the causative agent of amebic dysentery. metronidazole, a compound first used to treat t. vaginalis in 1959, is still the main drug used worldwide to treat these pathogens. it is essential to find new biochemical differences in these organisms that could be exploited to develop new antiprotozoal chemotherapeutics. recent ... | 2009 | 21426030 |
| high throughput genome-wide survey of small rnas from the parasitic protists giardia intestinalis and trichomonas vaginalis. | rna interference (rnai) is a set of mechanisms which regulate gene expression in eukaryotes. key elements of rnai are small sense and antisense rnas from 19 to 26 nt generated from double-stranded rnas. micrornas (mirnas) are a major type of rnai-associated small rnas and are found in most eukaryotes studied to date. to investigate whether small rnas associated with rnai appear to be present in all eukaryotic lineages, and therefore present in the ancestral eukaryote, we studied two deep-branchi ... | 2009 | 20333187 |
| a clinicopathological study of urethritis in males. | to determine the etiology and frequency of different types of urethritis in adult males. | 2009 | 20042155 |
| trichomonas vaginalis is highly prevalent in adolescent girls, pregnant women, and commercial sex workers in ndola, zambia. | objectives:: the aim of the study was to assess the prevalence of trichomonas sp. infection among adolescent girls, pregnant women, and commercial sex workers in ndola, zambia. methods:: a cross-sectional study was conducted among 460 girls attending school, 307 pregnant women, and 197 commercial sex workers. self-collected specimens from the vagina, rectum, and mouth were tested by polymerase chain amplification assays for the presence of trichomonas vaginalis, pentatrichomonas hominis, and tri ... | 2009 | 19940808 |
| the hydrogenosomes of psalteriomonas lanterna. | hydrogenosomes are organelles that produce molecular hydrogen and atp. the broad phylogenetic distribution of their hosts suggests that the hydrogenosomes of these organisms evolved several times independently from the mitochondria of aerobic progenitors. morphology and 18s rrna phylogeny suggest that the microaerophilic amoeboflagellate psalteriomonas lanterna, which possesses hydrogenosomes and elusive "modified mitochondria", belongs to the heterolobosea, a taxon that consists predominantly o ... | 2009 | 20003182 |
| time from first intercourse to first sexually transmitted infection diagnosis among adolescent women. | to determine the time between first intercourse and first sexually transmitted infection (sti) with chlamydia trachomatis, neisseria gonorrhoeae, or trichomonas vaginalis and time between repeated infections. | 2009 | 19996047 |
| maternal morbidity in the first year after childbirth in mombasa kenya; a needs assessment. | in sub-saharan africa, few services specifically address the needs of women in the first year after childbirth. by assessing the health status of women in this period, key interventions to improve maternal health could be identified. there is an underutilised opportunity to include these interventions within the package of services provided for woman-child pairs attending child-health clinics. | 2009 | 19891784 |
| malate dehydrogenase is negatively regulated by mir-1 in trichomonas vaginalis. | micrornas are highly conserved small noncoding rnas that can suppress protein translation through complementary binding to target mrnas. we used a novel approach to identify mirna targets in the protist trichomonas vaginalis by comparing the levels of differentially expressed proteins and genes in the trophozoite and amoeboid stages. we observed that the t. vaginalis malate dehydrogenase (tv_mdh) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold. b ... | 2009 | 19777264 |
| molecular characterization of trichomonas vaginalis isolates from the philippines. | trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral parasitic sexually transmitted infection in the world. presently, there are no reports on comparative sequence analysis as well as on the identification of phylogenetic positions of t. vaginalis isolates from the philippines relative to known trichomonads. in this study, 5.8s rdna and the flanking internal transcribed spacer (its) regions of 57 t. vaginalis isolates were sequenced. the phylo ... | 2009 | 19779740 |
| use of injectable progestin contraception and risk of sti among south african women. | this study was conducted to determine the association between the use of injectable progestin contraception (ipc) and the risk of infection with neisseria gonorrhoeae (gc), chlamydia trachomatis (ct), bacterial vaginosis (bv) and trichomonas vaginalis (tv) among women in south africa. | 2009 | 19913149 |
| metronidazole resistance in trichomonas vaginalis from highland women in papua new guinea. | the prevalence of the sexually transmissible protozoan parasite trichomonas vaginalis in the highlands of papua new guinea (png) has been reported to be as high as 46% and although not previously studied in papua new guinea, clinical resistance against metronidazole (mz), the drug most commonly used to treat trichomoniasis, is well documented worldwide. this study was primarily aimed at assessing resistance to mz in t. vaginalis strains from the goroka region. | 2009 | 19917203 |
| prevalence of trichomonas vaginalis infection among young reproductive age women in india: implications for treatment and prevention. | trichomonas vaginalis infection is the most common curable sexually transmissible infection (sti) worldwide. the present study describes the burden and correlates of t. vaginalis infection among young reproductive age women in mysore, india. | 2009 | 19917204 |
| treatment of metronidazole-resistant trichomonas vaginalis. | a 58-year-old caucasian woman presented with a 14-month history of persistent trichomoniasis not responsive to numerous courses of metronidazole and tinidazole. vaginal secretion samples were obtained for sensitivity testing and treatment recommendations. in vitro susceptibility testing revealed the patient's trichomonas vaginalis isolate was highly resistant to both metronidazole and tinidazole. she was treated with topical furazolidone and experienced a complete symptomatic cure and culture re ... | 2009 | 19917205 |
| prevalence of sexually transmitted infections among female adolescents aged 14 to 19 in the united states. | most young women initiate sexual activity during adolescence; risk for sexually transmitted infections (stis) accompanies this initiation. in this study we estimated the prevalence of the most common stis among a representative sample of female adolescents in the united states. | 2009 | 19933728 |
| idiopathic urethritis in young men in the united states: prevalence and comparison to infections with known sexually transmitted pathogens. | urethritis is the most common male reproductive tract disease syndrome; yet 20-50% of diagnosed cases have no defined etiology, and few population-level data exist on the prevalence or etiology of the syndrome. we estimated the prevalence of urethritis among young men in the united states and compared correlates of idiopathic cases to correlates of detected infections with sexually transmitted pathogens. | 2009 | 19837352 |
| partial purification and characterization of trichomonas vaginalis dna topoisomerase ii. | dna topoisomerases regulate conformational changes in dna topology by catalyzing the breakage and rejoining of dna strands during the cell cycle. these processes are essential for the multiplication of cells, and inhibition of these reactions stops cell division and cell growth. drug resistance to trichomonas vaginalis, a common sexually transmitted protozoan parasite, is increasing worldwide, and dna topoisomerase ii may provide a new target for anti-trichomonal drug development. in this study, ... | 2009 | 19842367 |
| the prevalence of sexually transmitted and other lower reproductive tract infections among rural women in sichuan province, china. | to estimate the prevalence of sexually transmitted infections (sti) and lower reproductive tract infections (rti) and determine risk factors for sti among rural women in sichuan province, china, a cross-sectional, community-based cluster sample of 2,000 rural, married women were interviewed, examined and clinical specimens collected to assess for six sti and two non-sexually transmitted rti. the overall prevalence of any sti was 10.9% (95% ci 9.5-12.3); of any sti or rti was 30.8% (95% ci 28.7-3 ... | 2009 | 19842387 |
| impact of t. vaginalis infection on innate immune responses and reproductive outcome. | trichomonas vaginalis is the most common non-viral sexually transmitted pathogen. the infection is prevalent in reproductive age women and is associated with vaginitis, endometritis, adnexitis, pyosalpinx, infertility, preterm birth, low birth weight, bacterial vaginosis, and increased risk of cervical cancer, hpv, and hiv infection. in men, its complications include urethritis, prostatitis, epididymitis, and infertility through inflammatory damage or interference with the sperm function. the in ... | 2009 | 19850356 |
| [in vitro effect of secnidazole benzoate on trichomonas vaginalis]. | to research the trichomonacidal effect of secnidazole benzoate in vitro. | 2009 | 19856497 |
| methionine gamma-lyase: the unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers. | sulfur-containing amino acids (saas) are essential components in many biological processes and ubiquitously distributed to all organisms. both biosynthetic and catabolic pathways of saas are heterogeneous among organisms and between developmental stages, and regulated by the environmental changes. limited lineage of organisms ranging from archaea to plants, but not human, possess a unique enzyme methionine gamma-lyase (mgl, ec 4.4.1.11) to directly degrade saa to alpha-keto acids, ammonia, and v ... | 2009 | 19859976 |
| synthesis and biological evaluation of new heterocyclic quinolinones as anti-parasite and anti-hiv drug candidates. | we have synthesized quinolinones with potential antiparasitic and anti-hiv activities by an original two-step method involving microwave irradiation and have evaluated their activities against plasmodium falciparum, leishmania donovani, trichomonas vaginalis, and hiv. none of the tested compounds had been previously described using this method of synthesis. one of the compounds had interesting antiparasitic and anti-hiv activity, which could be improved by substitution with different radicals. | 2009 | 19748781 |
| steroid hormones alter amp hydrolysis in intact trophozoites of trichomonas vaginalis. | trichomonas vaginalis infection may be influenced by the vaginal concentrations of estrogens. we have investigated the effects of 17beta-estradiol and dehydroepiandrosterone sulfate (dheas) on the ecto-5'-nucleotidase activity in fresh clinical (vp60) and in long-term-grown (30236 atcc) isolates of t. vaginalis. in vitro exposure to dheas and 17beta-estradiol did not induce any changes in adenosine monophosphate (amp) hydrolysis in these isolates. the treatment of parasites in the presence of dh ... | 2009 | 19756747 |
| the evolution of an integrated sexual health service. | the objective of the study was to assess the contributions of different providers across an integrated sexual health-care network to the diagnosis of sexually transmitted infections (stis). the method employed was retrospective cross-sectional survey of laboratory tests for chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis between 01 april 2004 and 31 march 2007. the total number of sti screens in all venues rose from 17,126 to 23,679. the proportion of sti screens occurring ... | 2009 | 19759047 |
| the mercaptopyruvate sulfurtransferase of trichomonas vaginalis links cysteine catabolism to the production of thioredoxin persulfide. | trichomonas vaginalis is a protozoan parasite of humans that is able to synthesize cysteine de novo using cysteine synthase but does not produce glutathione. in this study, high pressure liquid chromatography analysis confirmed that cysteine is the major intracellular redox buffer by showing that t. vaginalis contains high levels of cysteine ( approximately 600 mum) comprising more than 70% of the total thiols detected. to investigate possible mechanisms for the regulation of cysteine levels in ... | 2009 | 19762467 |
| male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis: observations after a randomised controlled trial for hiv prevention. | to assess the association between male circumcision and neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis using data from a male circumcision randomised controlled trial. | 2009 | 19074928 |
| in vitro testing of rationally designed spermicides for selectively targeting human sperm in vagina to ensure safe contraception. | rational synthesis of novel structures resulted in two unique molecules (dse-36 and dse-37, disulphide esters of carbothioic acid) that killed sperm 25 times more strongly and with a precisely targeted action than nonoxynol-9 (n-9). we examine the effects of dse-36 and dse-37 on human spermatozoa versus hela cells to establish specificity and safety compared with n-9. | 2009 | 19088111 |
| an iatrogenic confounding variable. | 2009 | 19741210 | |
| prospective study of trichomonas vaginalis infection and prostate cancer incidence and mortality: physicians' health study. | a recent nested case-control study found that the presence of antibodies against trichomonas vaginalis, a common nonviral sexually transmitted infection, was positively associated with subsequent incidence of prostate cancer. we confirmed these findings in an independent population and related serostatus for antibodies against t vaginalis to prostate cancer incidence and mortality. | 2009 | 19741211 |
| proinflammatory cytokine and nitric oxide production by human macrophages stimulated with trichomonas vaginalis. | trichomonas vaginalis commonly causes vaginitis and perhaps cervicitis in women and urethritis in men and women. macrophages are important immune cells in response to t. vaginalis infection. in this study, we investigated whether human macrophages could be involved in inflammation induced by t. vaginalis. human monocyte-derived macrophages (hmdm) were co-cultured with t. vaginalis. live, opsonized-live trichomonads, and t. vaginalis lysates increased proinflammatory cytokines, such as tnf-alpha, ... | 2009 | 19724692 |
| trichomoniasis and other sexually transmitted infections: results from the 2001-2004 national health and nutrition examination surveys. | to estimate the association between trichomonas vaginalis infection (tv) and 6 sexually transmitted infections: chlamydia, gonorrhea, herpes simplex virus (types 1 and 2), syphilis, and human immunodeficiency virus (hiv) in a nationally representative sample. | 2009 | 19734826 |
| trichomonal sinusitis in an adolescent patient with multiple trauma. | trichomonal disease typically involves the genital and occasionally respiratory tracts. although exposure of the upper respiratory tract to infected genital secretions is not uncommon with contemporary sexual practices, trichomonal sinus disease has been rarely described. the present report describes the case of a healthy 17-year-old male admitted to an intensive care unit following multiple trauma, who developed purulent sinusitis on the 4th day of hospitalization. numerous trichomonads were no ... | 2009 | 19204647 |
| synthesis and in vitro trichomonicidal, giardicidal and amebicidal activity of n-acetamide(sulfonamide)-2-methyl-4-nitro-1h-imidazoles. | two new series of imidazole derivatives (acetamides: 1-8 and sulfonamides: 9-15) were synthesized using a short synthetic route. compound 1 as well as the intermediate 16g were characterized by x-ray crystallography. imidazole derivatives 1-15 were tested in vitro against three unicellular parasites (giardia intestinalis, trichomonas vaginalis and entamoeba histolytica) in comparison with benznidazole (bzn) and metronidazole. compound 1 [n-benzyl-2-(2-methyl-4-nitro-1h-imidazol-1-yl)acetamide] w ... | 2009 | 19208443 |
| comparison of clinical symptoms scored according to the national institutes of health chronic prostatitis symptoms index and assessment of antimicrobial treatment in patients with chronic prostatitis syndrome. | we examined a total of 194 patients over 18 years of age with chronic prostatitis syndrome and no evidence of structural or functional lower genitourinary tract abnormalities. the following data were obtained for each patient: clinical history--the severity of chronic prostatitis symptoms scored by a croatian translation of the nih cpsi questionnaire, clinical status including digitorectal examination, urethral swab specimens, and selective samples of urine and expressed prostatic secretion, acc ... | 2009 | 19423471 |
| disentangling contributions of reproductive tract infections to hiv acquisition in african women. | : to estimate the effects of reproductive tract infections (rtis) on hiv acquisition among zimbabwean and ugandan women. | 2009 | 19434010 |
| identification of microrna in the protist trichomonas vaginalis. | micrornas (mirnas) are a class of small noncoding rnas that have important regulatory roles in multicellular organisms. however, mirna has never been identified experimentally in protist. direct cloning of 438 expressed mirna tags by microrna serial analysis of gene expression from the parasitic protist trichomonas vaginalis identified nine candidate mirnas. bioinformatics analysis of the corresponding genomic region revealed that these mirna candidates contain a classical stem-loop-stem structu ... | 2009 | 19442639 |
| examination for double-stranded rna viruses in trichomonas gallinae and identification of a novel sequence of a trichomonas vaginalis virus. | to determine if double-stranded rna (dsrna) viruses exist and are potential virulence factors in trichomonas gallinae, virus purification via ultracentrifugation was attempted for 12 t. gallinae isolates recovered from wild birds. following purification, virus-like particles were not observed by transmission electron microscopy, nor were dsrna segments visualized in agarose gels after electrophoresis of extracted rna from any of the 12 t. gallinae isolates. however, virus particles and dsrna seg ... | 2009 | 19452169 |
| anal sex is a behavioural marker for laboratory-confirmed vaginal sexually transmissible infections and hiv-associated risk among african-american female adolescents. | african-american females are disproportionately affected by hiv and sexually transmissible infections (stis). the prevalence of anal sex and its association with other sexual risk behaviours is understudied in this population. | 2009 | 19457289 |
| in vitro activities of three synthetic peptides derived from epinecidin-1 and an anti-lipopolysaccharide factor against propionibacterium acnes, candida albicans, and trichomonas vaginalis. | the synthetic epinecidin-1(22-42) peptide was derived from positions 22-42 of epinephelus coioides epinecidin-1. the synthetic salf(55-76) cyclic peptide (cssalf(55-76)) and salf(55-76) linear peptide (lssalf(55-76)) contained sequences from positions 55 to 76 of the penaeus monodon anti-lipopolysaccharide factor (alf), respectively. we studied the in vitro activities of epinecidin-1(22-42), cssalf(55-76), and lssalf(55-76) against propionibacterium acnes, candida albicans, and trichomonas vagin ... | 2009 | 19463737 |
| bacterial vaginosis in female facility workers in north-western tanzania: prevalence and risk factors. | to determine prevalence of, and risk factors for, bacterial vaginosis (bv) among herpes simplex virus (hsv) 2 seropositive tanzanian women at enrollment into a randomised, placebo-controlled trial of hsv suppressive treatment. | 2009 | 19473997 |
| trichomoniasis in teens: an update. | trichomoniasis, caused by the protozoa trichomonas vaginalis, is one of the oldest sexually transmitted infections. since the advent of more accurate diagnostic tests, the epidemiology and consequences of infection with t. vaginalis can be described more precisely. this review will highlight new diagnostic methods, the epidemiology of trichomoniasis, and discuss the merits of improved screening for this pathogen in adolescent women. | 2009 | 19491679 |
| a study comparing sexually transmitted infections and hiv among ex-red-light district and non-red-light district sex workers after the demolition of baina red-light district. | in june 2004, the red-light area of goa was demolished. we compare hiv and sexually transmitted infections (stis) between sex workers who had been based in baina red-light district (ex-rld-sws) with sex workers who had never worked in baina (non-rld-sws). | 2009 | 19525855 |
| [leucoplakia vesicae in females: diagnosis and treatment]. | sixty patients suffering from leucoplakia vesicae (lv) were examined using cystoscopy with biopsy of the urinary bladder wall, blood enzyme immunoassay for detection of antibodies to agents of sexually transmitted infections (sti), uroflowmetry, culture analysis of cervical canal and mucosa samples for sti. as shown by a pathomorphological examination of the vesical mucosa biopsy specimens, long-term persistence of pathogenic (chlamydia, trichomonades) and opportunistic (mycoplasma, ureaplasma, ... | 2009 | 19526870 |
| polymerase chain reaction versus conventional methods in the diagnosis of vaginal trichomoniasis. | a total of 200 females of whom 120 had manifestations of vaginal trichomoniasis and 80 asymptomatic ones were studied. in 54/120 symptomatic female (45%) and in 28/80 asymptomatic ones (35%), t. vaginalis was diagnosed by wet mount of bedside vaginal swab samples. of 120 samples from symptomatic females, t. vaginalis was detected in 93 (77.5%) when cultured onto inpouch & 95 (79.16%) in modified thioglycolate media. culturing 80 samples of asymptomatic females showed t. vaginalis in 35 (43.75%) ... | 2009 | 19530606 |
| efficacy of two plant extracts against vaginal trichomoniasis. | trichomoniasis vaginalis is now an important worldwide health problem. metronidazole has so far been used in treatment, but the metronidazole-resistant strains and unpleasant adverse effects have been developed. treatment of patients with metronidazole refractory vaginal trichomoniasis constitutes a major therapeutic challenge and treatment options are extremely limited. in the present study, 33 metronidazole-resistant t. vaginalis females were treated with a combined course of metronidazole and ... | 2009 | 19530609 |
| responsiveness of trichomonas vaginalis to iron concentrations: evidence for a post-transcriptional iron regulation by an ire/irp-like system. | trichomonas vaginalis has high iron-dependency, favoring its growth and multiplication in culture. iron also regulates some of the trichomonal virulence properties by yet unknown mechanisms. iron is an essential but potentially toxic metal for the majority of organisms. thus, its concentration must be tightly regulated within the cell. in mammals, the iron homeostasis is mainly regulated at the post-transcriptional level by a well known mechanism mediated by the binding of iron regulatory protei ... | 2009 | 19539055 |
| [tinidazole: a classical anaerobical drug with multiple potential uses nowadays]. | tinidazole is a 5-nitroimidazole active in vitro against a wide variety of anaerobic bacteria and protozoa. tinidazole is an effective treatment against anaerobic microorganisms based on its pharmacokinetic characteristics (c(max) 51 microg/ml, t(1/2) 12.5 h) and its excellent in vitro activity. its long half-life allows once a day regimens. tinidazole is as effective as metronidazole in the treatment of infections caused by t. vaginalis, giardiasis and amebiasis and bacterial vaginosis, malaria ... | 2009 | 19544102 |
| adult male circumcision does not reduce the risk of incident neisseria gonorrhoeae, chlamydia trachomatis, or trichomonas vaginalis infection: results from a randomized, controlled trial in kenya. | we examined the effect of male circumcision on the acquisition of 3 nonulcerative sexually transmitted infections (stis). | 2009 | 19545209 |
| redox properties of the oxygen-detoxifying flavodiiron protein from the human parasite giardia intestinalis. | flavodiiron proteins (fdps) are enzymes identified in prokaryotes and a few pathogenic protozoa, which protect microorganisms by reducing o(2) to h(2)o and/or no to n(2)o. unlike most prokaryotic fdps, the protozoan enzymes from the human pathogens giardia intestinalis and trichomonas vaginalis are selective towards o(2). uv/vis and epr spectroscopy showed that, differently from the no-consuming bacterial fdps, the giardia fdp contains an fmn with reduction potentials for the formation of the si ... | 2009 | 19545535 |
| the association between oral contraceptives, depot-medroxyprogesterone acetate, and trichomoniasis. | hormonal contraception use by women may increase the risk of acquiring certain sexually transmitted infections. we explored the effect of hormonal contraceptive use, specifically oral contraception (oc), and depot-medroxyprogesterone acetate (dmpa) on trichomonas vaginalis infections in women. | 2009 | 19556926 |
| vaginal and endocervical microorganisms in symptomatic and asymptomatic non-pregnant females: risk factors and rates of occurrence. | physiological or non-physiological factors may affect the vaginal flora. the occurrence of genital microorganisms in non-pregnant females of all ages was studied, as were the risk factors associated with each microorganism. a retrospective analysis of vaginal and endocervical cultures and wet smears from 27,172 non-pregnant women, between 1996 to 2005, was performed taking into consideration clinical and socio-demographic characteristics. no microorganisms were observed in 55.7% of the individua ... | 2009 | 19558525 |
| epidemiology of sexually transmitted infections in suspected child victims of sexual assault. | the objective of this study was to describe the epidemiology of neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, treponema pallidum, hiv, and herpes simplex virus type 2 (hsv-2) infection diagnosed by culture or by serologic or microscopic tests and by nucleic acid amplification tests in children who are evaluated for sexual victimization. | 2009 | 19564286 |
| diagnosis of trichomonas vaginalis in female children and adolescents evaluated for possible sexual abuse: a comparison of the inpouch tv culture method and wet mount microscopy. | to compare the performance of wet mount microscopy and the inpouch tv culture method in diagnosing trichomonas vaginalis in a cohort of female children and adolescents evaluated for possible sexual abuse. | 2009 | 19576816 |
| depression, sexually transmitted infection, and sexual risk behavior among young adults in the united states. | to measure associations among depression, sexual risk behaviors, and sexually transmitted infection (sti) among white and black youth in the united states. | 2009 | 19581548 |
| trichomonas vaginalis infection and associated risk factors in a socially-marginalized female population in coastal peru. | the epidemiology of trichomonas vaginalis infection among sexually active socially-marginalized women in three urban, coastal peruvian cities was examined in order to quantify the prevalence of trichomonas infection and identify associated risk factors. | 2009 | 19584943 |
| a quantitative resazurin assay to determinate the viability of trichomonas vaginalis and the cytotoxicity of organic solvents and surfactant agents. | trichomonas vaginalis causes trichomonosis, the most common, non-viral sexually transmitted disease. to test anti-trichomonas agents, usually many with low water solubility, organic solvents and surfactant agents should be used. therefore, the minimal inhibitory concentration (mic) of acetone, methanol, ethanol, isopropanol, dmso, tween 20, tween 80, and triton x-100 was determined against t. vaginalis isolates using the quantitative resazurin method. our results showed that solvents and surfact ... | 2009 | 19619538 |
| the protist trichomonas vaginalis harbors multiple lineages of transcriptionally active mutator-like elements. | for three decades the mutator system was thought to be exclusive of plants, until the first homolog representatives were characterized in fungi and in early-diverging amoebas earlier in this decade. | 2009 | 19622157 |
| physical health consequences of sexual assault victims. | to describe health consequences of sexual assault victims attending police general hospital, thailand. | 2009 | 19626805 |
| correlates of sexually transmitted infection histories in a cohort of american male health professionals. | several epidemiologic studies have investigated sexually transmitted infections (stis) and later risk of genitourinary conditions with suggestive positive results. while these results may reflect causal associations, other possible explanations include confounding by factors possibly related to both sti acquisition and genitourinary condition risk such as recognized sti-risk factors/correlates, and other factors not typically considered in relation to stis (e.g., general health-related behaviors ... | 2009 | 19655261 |
| mycoplasma genitalium as a contributor to the multiple etiologies of cervicitis in women attending sexually transmitted disease clinics. | the purpose of this study was to investigate the prevalence of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and mycoplasma genitalium, in women attending a sexually transmitted disease (std) clinic, as well as the frequency of coinfections, and relationship of each organism to cervicitis. | 2009 | 19704398 |
| [cloning, expression and immunogenicity analysis of cysteine proteinase 3 of trichomonas vaginalis]. | to study the humoral antibody response of mice to the recombinant trichomonas vaginalis cysteine proteinase (tvcp3) in order to investigate the function of the proteinase and its application in diagnosis. | 2009 | 20232629 |
| urinary tract infection among asymptomatic hiv patients in benin city, nigeria. | the impact of human immunodeficiency virus (hiv) infection and cd4+ count on the prevalence of urinary tract infection (uti) is studied to determine the prevalence of uti among hiv and non-hiv subjects. clean-catch midstream urine and venous blood was collected from 421 subjects comprising 317 hiv patients (89 men, 228 women) and 104 non-hiv subjects (48 men, 56 women). the hiv patients consisted of 101 highly active antiretroviral therapy (haart)-naive subjects and 216 patients on haart for thr ... | 2009 | 20095127 |
| [microbiological diagnosis of sexually transmitted infections (sti): part 1. non-viral sti]. | non-viral sexually transmitted infections (sti) are an important cause of physical, psychological and social distress, have severe consequences for women's reproductive health and may be transmitted to the newborn child. these infections are also risk factors for the acquisition and transmission of hiv and other sti, and for premature labor. in the last years we have observed a gradual decrease in the national incidence of gonorrhea. the implementation of a screening program in our country for c ... | 2009 | 20098788 |
| in vitro activity of arbutus unedo leaf extracts against trichomonas vaginalis trophozoites. | trichomonas vaginalis (t. vaginalis) is a flagellated protozoan commonly causing sexually transmitted disease. t. vaginalis infections are treated with a 5-nitroimidazole derivate. however, drug resistance has been known to occur for a long time and new alternatives are under investigation. arbutus unedo is a wild plant mainly growing in maquis and rocky places of the seaboard in southern europe. in our study, ethanolic, water, hexane and ethyl acetate extracts of arbutus unedo leaves were teste ... | 2009 | 20101573 |
| [the relation between trichomoniasis and contraceptive methods]. | trichomoniasis is a sexually transmitted disease caused by trichomonas vaginalis (t. vaginalis) infecting the urogenital system. in this study, the relation between different contraceptive methods used and t. vaginalis infection was investigated. a total of 253 women (aged from 20-48 years) with abnormal vaginal discharges who applied to the obstetrics and gynecology outpatient clinic were enrolled in the study. t. vaginalis was diagnosed by microscopic examination of direct and giemsa stained p ... | 2009 | 20101574 |
| evaluation of common organisms causing vaginal discharge. | vaginal discharge is very common problem among females. alteration in balance of normal vaginal organisms can cause the overgrowth of the bacteria that creates vaginal discharge. it is common among sexually active women yet there still remain gaps in our knowledge of this infectious disorder. | 2009 | 20524479 |
| infectivity of trichomonas vaginalis pseudocysts inoculated intra-vaginally in mice. | in addition to the trophozoite, pseudocyst is another morphological form which is recently identified among genitourinary trichomonads. although, this pseudocyst is competent to divide, its role in trichomonas life cycle has not yet been confirmed. in this study the ability of intra-vaginally inoculated t. vaginalis pseudocysts to induce trichomoniasis in infected mice was evaluated in comparison to the trophozoites. pseudocysts formation was induced by using thermal-freezing cycle method. the i ... | 2008 | 19209760 |
| comparison between trichmonas vaginalis symptomatic and asymptomatic isolates effects in intravaginally infected rats. | in this study, histopathological and immunohistochemical changes of the posterior vaginal fornix's and upper portion of the vagina were compared on rats infected with symptomatic and asymptomatic human isolates. eighteen symptomatic and asymptomatic female isolates were used (nine/ each). two groups of infected female rats were included in this study (3 rats /isolate). the results showed that there were no differences between symptomatic and asymptomatic isolates in histopathological changes; t. ... | 2008 | 19209767 |
| risk factors for sexually transmitted infections in women in rural northeast brazil. | sexually transmitted infections (stis) are highly prevalent in northeast brazil, but factors associated with the presence of an sti have rarely been studied systematically. | 2008 | 19738353 |
| reductive evolution of the mitochondrial processing peptidases of the unicellular parasites trichomonas vaginalis and giardia intestinalis. | mitochondrial processing peptidases are heterodimeric enzymes (alpha/betampp) that play an essential role in mitochondrial biogenesis by recognizing and cleaving the targeting presequences of nuclear-encoded mitochondrial proteins. the two subunits are paralogues that probably evolved by duplication of a gene for a monomeric metallopeptidase from the endosymbiotic ancestor of mitochondria. here, we characterize the mpp-like proteins from two important human parasites that contain highly reduced ... | 2008 | 19096520 |
| trichomoniasis. an update on diagnostic tools and treatments. | 2008 | 19999494 | |
| [steroidal saponins from the plant agave brittoniana with activity against the parasite trichomona vaginalis]. | the genus agave (agavaceae), includes more than 300 species; around 16 of them show an homogeneous distribution throughout cuba. agave brittoniana (ssp. brachypus), is an endemic subspecies that grows in the central region of the country and its leaves are traditionally used in the treatment of parasitic diseases. the parasite trichomonas vaginalis causes the disease known as trichomoniasis, that infects the genital tract. to test in vitro the plant against trichomona vaginalis, the dried and po ... | 2008 | 19419073 |
| prevalence of vulvovaginitis and bacterial vaginosis in patients with koilocytosis. | empirical discussion regarding an association between koilocytosis and vulvovaginitis often occurs. thus, the objective of this study was to assess the prevalence of microorganisms associated with bacterial vaginosis and vulvovaginitis in women with and without koilocytosis. | 2008 | 19274321 |
| [utility of molecular biology techniques in the diagnosis of sexually transmitted diseases and genital infections]. | historically, the diagnosis of sexually transmitted diseases (stds) has been difficult. the introduction of molecular biology techniques in microbiological diagnosis and their application to non-invasive samples has produced significant advances in the diagnosis of these diseases. overall, detection of neisseria gonorrhoeae by molecular biology techniques provides a presumptive diagnosis and requires confirmation by culture in areas with a low prevalence. for chlamydia trachomatis infections, th ... | 2008 | 19195446 |
| a comparison of cytological and parasitological methods in the diagnosis of trichomonas vaginalis. | trichomonas vaginalis (t. vaginalis), which causes urogenital system infections in humans, develops symptomatically and asymptomatically. t. vaginalis in females is diagnosed using direct microscopy, giemsa staining, and cultivation methods for examination of samples derived from the vaginal posterior fornix. serologic methods can also be employed. in cytological diagnosis, the ectocervical smear is examined using the papanicolaou (paps) stain. the aim of the present study was to compare the eff ... | 2008 | 19156601 |
| [killing effect of polymorphonuclear neutrophils on trichomonas vaginalis]. | to study the killing effect of polymorphonuclear neutrophils (pmns) on trichomonas vaginalis. | 2008 | 19157301 |
| [observation on mitosis of trichomonas vaginalis cultivated in vitro using modified giemsa staining]. | the observation showed that the percentage of trichomonas vaginalis trophozoites at the stages of interphase, binary fission and multiple fission was 66.5%, 24.1% and 9.4% respectively. cells in binary fission could be classified as premitotic phase, prophase, metaphase, anaphase and telophase. 3 to 8 microcosms were seen in one trophozoite under multiple fission and the percentage of trophozoites with 3 and 4 microcosms occupied 69% and 24.5% respectively. cells with abnormal morphs were also o ... | 2008 | 19157311 |
| [genetic variation and clustal analysis of trichomonas vaginalis cysteine proteases]. | to clone the genes coding for cysteine proteases (cps, tvcps) from trichomonas vaginalis and to analyze their genetic variations with the related sequences from ncbi database (genbank) and t. vaginalis genome project database from the institute for genomic research (tigr). | 2008 | 19160965 |
| prevalence of mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in bangladesh. | the objective of the study was to determine the prevalence of mycoplasma genitalium in a sample of health clinic attendees complaining of vaginal discharge. a subsample of 399 vaginal and cervical swabs was randomly selected from 2579 samples collected during a study to determine the causes of vaginal discharge in women attending primary health-care clinics in dhaka, bangladesh. cervical samples were tested for m. genitalium by polymerase chain reaction. in addition, the samples were tested for ... | 2008 | 18931272 |
| enzymatic assay for total plasma cys. | patients with vascular disease and end-stage renal disease have significantly higher concentrations of plasma total cys (tcys) than do healthy individuals. described here is a nonradioactive, precise, rapid and sensitive enzymatic colorimetric assay for tcys in plasma samples and is homogeneous in that it avoids separation methods. the tcys assay uses only the recombinant enzymes methionine alpha,gamma-lyase (rmetase) and s-adenosylhomocysteine hydrolase (rsahh) cloned from pseudomonas putida an ... | 2008 | 18948977 |
| the divergent eukaryote trichomonas vaginalis has an m7g cap methyltransferase capable of a single n2 methylation. | eukaryotic rnas typically contain 5' cap structures that have been primarily studied in yeast and metazoa. the only known rna cap structure in unicellular protists is the unusual cap4 on trypanosoma brucei mrnas. we have found that t. vaginalis mrnas are protected by a 5' cap structure, however, contrary to that typical for eukaryotes, t. vaginalis spliceosomal snrnas lack a cap and may contain 5' monophophates. the distinctive 2,2,7-trimethylguanosine (tmg) cap structure usually found on snrnas ... | 2008 | 18957443 |
| vulnerability of women in southern africa to infection with hiv: biological determinants and priority health sector interventions. | to review biomedical determinants of women's vulnerability to infection with hiv and interventions to counter this, within the southern african context. | 2008 | 19033753 |
| evaluation of selected tropical seaweeds for in vitro anti-trichomonal activity. | human parasitic infections are a serious problem in tropical and sub-tropical developing countries. trichomoniasis, responsible for the annual infection of 180 million people, is a common sexually transmitted disease caused by the protozoan trichomonas vaginalis. traditionally seaweeds have been used in folk medicine by coastal people in asia and the caribbean to treat parasitic infections and are a valuable source of novel anti-trichomonals. | 2008 | 18725281 |
| how men with non-chlamydial, non-gonococcal urethritis are managed in australasia. | the aim of this study was to ascertain how sexual health physicians in australia and new zealand manage men with chlamydia-negative non-gonococcal urethritis (ngu), particularly in relation to the notification of their female sexual partners. in july 2006, a cross-section survey was sent out to all the members of the australasian chapter of sexual health medicine. seventy-three percent of sexual health physicians believed that female partners of men who present with chlamydia-negative ngu were a ... | 2008 | 18725546 |
| measuring the gap: from home office to the national health service in the provision of a one-stop shop sexual health service in a female prison in the uk. | the genitourinary (gu) medicine service was transferred from the home office to the nhs from april 2006 at this female prison to give prisoners access to the same quality of health care as the general public. medline search showed no published data on the prevalence of sexually transmitted infections (stis) among female prisoners in the uk. the main aim was to develop a one stop sexual health shop and to determine the prevalence and risk factors for stis, to determine the uptake rate for hiv tes ... | 2008 | 18725547 |
| prevalence and predictors of herpes simplex virus type 2 infection among female sex workers in yunnan province, china. | the objective of this study was to determine the seroprevalence of herpes simplex virus type 2 (hsv-2), and to evaluate the relationship between hsv-2 infection and sociodemographic factors and the sexual practices of female sex workers (fsws) in kaiyuan city, yunnan province, china. this cross-sectional study involved 737 fsws and was carried out from march to may 2006 with confidential interviews and laboratory tests for hsv-2 and other sexually transmitted infections (sti). hsv-2 was the most ... | 2008 | 18725558 |
| reassessment of bv causation. | 2008 | 18725563 | |
| impact of trichomonas vaginalis transcription-mediated amplification-based analyte-specific-reagent testing in a metropolitan setting of high sexually transmitted disease prevalence. | trichomoniasis is a significant sexually transmitted disease (std) in the spectrum of public health and primary care because of its association with agents such as human immunodeficiency virus and neisseria gonorrhoeae. however, its true significance may be underestimated due to diagnostic modalities that exhibit poor sensitivity. a total of 1,086 genital specimens from two urban emergency departments, a suburban urgent-care facility, and a metropolitan outpatient physician group were subjected ... | 2008 | 18753347 |
| new antitrichomonal drug-like chemicals selected by bond (edge)-based tomocomd-cardd descriptors. | bond-based quadratic indices, new tomocomd-cardd molecular descriptors, and linear discriminant analysis (lda) were used to discover novel lead trichomonacidals. the obtained lda-based quantitative structure-activity relationships (qsar) models, using nonstochastic and stochastic indices, were able to classify correctly 87.91% (87.50%) and 89.01% (84.38%) of the chemicals in training (test) sets, respectively. they showed large matthews correlation coefficients of 0.75 (0.71) and 0.78 (0.65) for ... | 2008 | 18753687 |
| biochemical characterization of a mitochondrial-like organelle from blastocystis sp. subtype 7. | a mitochondrion-like organelle (mlo) was isolated from isotonic homogenates of blastocystis. the organelle sedimented at 5000 g for 10 min, and had an isopycnic density in sucrose of 1.2 g ml(-1). biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete krebs cycle linked to the oxygen-sensitive enzymes pyruvate : nadp(+) oxidoreductase (pno), acetate : succinate coa transferase (asct) and succi ... | 2008 | 18757809 |
| co-occurrence of intoxication during sex and sexually transmissible infections among young african american women: does partner intoxication matter? | the co-occurrence of a behaviour (being intoxicated on alcohol/drugs during sex) with a disease outcome [laboratory-confirmed sexually transmissible infection (sti) prevalence] among young african american women and their male sex partners was studied. | 2008 | 18771645 |
| a novel dna sequence periodicity decodes nucleosome positioning. | there have been two types of well-characterized dna sequence periodicities; both are found to be associated with important molecular mechanisms. one is a 3-nt periodicity corresponding to codon triplets, the other is a 10.5-nt periodicity related to the structure of dna helixes. in the process of analyzing the genome and transcriptome of trichomonas vaginalis, we observed a 120.9-nt periodicity along dna sequences. different from the 3- and 10.5-nt periodicities, this novel periodicity originate ... | 2008 | 18829715 |
| topical fenticonazole in dermatology and gynaecology: current role in therapy. | fenticonazole is an imidazole derivative with a broad spectrum of antimycotic activity against dermatophytes and yeasts in in vitro and clinical studies. fenticonazole exerts its unique antimycotic mechanism of action in the following three ways: (i) inhibition of the secretion of protease acid by candida albicans; (ii) damage to the cytoplasmic membrane; and (iii) by blocking cytochrome oxidases and peroxidises. fenticonazole has also been shown to exhibit antibacterial action, with a spectrum ... | 2008 | 18840006 |
| molecular typing of the actin gene of trichomonas vaginalis isolates by pcr-restriction fragment length polymorphism. | human trichomoniasis, caused by the protozoan trichomonas vaginalis, is a highly prevalent sexually transmitted infection. however, little is known about the degree of strain variability of t. vaginalis. a reliable classification method for t. vaginalis strains would be a useful tool in the study of the epidemiology, pathogenesis and transmission of t. vaginalis. a pcr-restriction fragment length polymorphism typing method was designed and evaluated using t. vaginalis isolates obtained after cul ... | 2008 | 18844685 |
| human urinary parasites in nalout popularity, libya. | a total of 2000 fresh urine samples were collected from a central private laboratory in tigi conference which served all the popularity. the examination showed motile trichomonas vaginalis in seven patients (0.35%); five females and two males, and enterobius vermicularis in two female children (0.1%). none had urinary schistosomiasis. the data were discussed. | 2008 | 18853614 |