Publications

in vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virus.		1976	936472
use of inactivating agents to detect and partially characterize very early events in the infection of cowpea leaf epidermal cells by peanut stunt virus.		1976	948863
nitrogenase activity and respiration of cultures of rhizobium spp. with special reference to concentrations of dissolved oxygen.	studies of nitrogenase in cultures of the cowpea rhizobia (rhizobium spp.) strains 32h1 and cb756 are reported. preliminary experiments established that, even when agar cultures were grown in air, suspensions of bacteria prepared anaerobically from them were most active at low concentrations of free dissolved o2. consequently, assays for activity used low concentrations of o2, stabilized by adding the nodule pigment leghaemoglobin. in continuous, glutamine-limited cultures of 32h1, nitrogenase a ...	1976	953022
homogenization-resistant and -susceptible components of tobacco mosaic virus replicative form rna.	when prepared from tissue frozen with liquid nitrogen, tobacco mosaic virus replicative form rna (tmv rf) was uniform in size but when prepared by high-speed homogenization, or when tmv rf prepared with liquid nitrogen was homogenized, 80 to 90% of the rf broke into relatively discrete pieces. the unbroken rf was not fragmented by additional homogenization. the tmv rf components susceptible and resistant to breakage, respectively, were synthesized with similar kinetics in relation to length of l ...	1976	1018172
comparison of chemical and microbiological methods in the estimation of methionine in cowpea (vigna unguiculata) seeds.	1. meals were prepared from the seeds of fifteen varieties of cowpea (vigna unguiculata), one of lima bean (phaseolus lunatus) and one of yam bean (sphenostylis stenocarpa), and their methionine content was determined by six different methods. 2. total methionine content was determined by two chemical methods (ion-exchange chromatography and a colorimetric procedure) and by two microbiological methods. the 'available' methionine content was determined by microbiological assay with streptococcus ...	1976	821514
an efficient mrna-dependent translation system from reticulocyte lysates.	a simple method is described for converting a standard rabbit reticulocyte cell-free extract (lysate) into an mrna-dependent protein synthesis system. the lysate is preincubated with cacl2 and micrococcal nuclease, and then excess ethyleneglycol-bis(2-aminoethylether)-n,n'-tetraacetic acid is added to chelate the ca2+ and inactivate the nuclease. lysates treated in this way have neglibible endogenous amino acid incorporation activity, but 75% of the activity of the original lysate can be recover ...	1976	823012
proceedings: the cowpea strain of tmv is a pseudo-multicomponent virus.		1976	1275404
nitrogenase activity in cultured rhizobium sp. strain 32h1: nutritional and physical considerations.	nutritional and physical conditions affecting nitrogenase activity in the strain of "cowpea" rhizobia, 32h1, were examined using cultures grown on agar medium. arabinose in the basic medium (cs7) could be replaced by ribose, xylose, or glycerol, but mannitol, glucose, sucrose, or galactose only supported low nitrogenase (c2h2 reduction) activity. succinate could be replaced by pyruvate, fumarate, malate, or 2-oxoglutarate, but without any carboxylic acid, nitrogenase activity was low or undetect ...	1976	5982
nitrogen fixation in cultured cowpea rhizobia: inhibition and regulation of nitrogenase activity.		1976	11803
investigation on the infection of cucumber mesophyll protoplasts with cucumber mosaic virus.	isolated protoplasts from the first leaf mesophyll of cucumber plants have been successfully infected in vitro with cucumber mosaic virus (cmv). virus instability before, during and after inoculation of the protoplasts resulted in low infectivities when extracts were assayed on cowpea; however, viral rna extraction improved the bioassay technique. attempts to optimize inoculation and incubation of protoplasts are outlined, incorporating the improved assay.	1976	13767
protozoa and the decline of rhizobium populations added to soil.	a fall in rhizobium abundance occurred in nonsterile soil inoculated with large numbers of the root-nodule bacteria, but many of the rhizobia still survived. no such decline was evident in sterile soil. protozoa feeding on these bacteria were isolated from soil and other environments. as the abundance of rhizobium meliloti and a cowpea rhizobium strain in soil decreased, the protozoan density increased. the inability of the predators to eliminate their prey from soil was not the result of the pr ...	1975	807307
the effect of double-stranded cowpea mosaic viral rna on protein synthesis.	the effect of double-stranded cowpea mosaic viral rna on several in vitro protein synthesizing systems was studied. no inhibitory effect of this rna at concentrations up to 16 mug/ml was found in wheat germ protein synthesis programmed with cowpea mosaic viral-, alfalfa mosaic viral- or globin 9 s-rna. at high concentrations of this double-stranded rna, inhibition of escherichia coli in vitro protein synthesis programmed with ms-2- and cowpea mosaic viral-rna was found, whereas at low concentrat ...	1975	1092369
the amino acid sequence of the cowpea strain of tobacco mosaic virus protein.	the amino acid sequence of the coat protein of the cowpea strain of tobacco mosaic virus (cowpea virus) has been determined. the tryptic peptide overlaps were obtained by digesting the protein with chymotrypsin and separating and analysing the lysine-and arginine-containing chymotryptic peptides. the primary structure of cowpea virus protein has been found to differ markedly from that of any other known strain of tobacco mosaic virus, and contains 3 amino acid residues more and 96 amino acid cha ...	1975	1138919
minor protein components in cowpea chlorotic mottle virus and satellite of tobacco necrosis virus.		1974	4414688
structure of isometric viruses containing nonidentical polypeptide chains.	the theory of caspar and klug (1962) for the structure of isometric viruses has been generalized to the case in which the identical repeating unit is composed of n nonidentical polypeptide chains. this modified theory accounts for the structure of picornaviruses, the lambda phage head, cowpea mosaic virus, and phix174, while at the same time conserving the principle of having identical subunits in identical environments. furthermore, the modified theory suggests amending the triangulation number ...	1974	4418754
the amino acid sequences of the tryptic peptides of the cowpea strain of tobacco mosaic virus protein.		1974	4441618
the localization of virus-specific double-stranded rna of cowpea mosaic virus in subcellular fractions of infected vigna leaves.		1973	4576343
the infection of tobacco protoplasts with cowpea chlorotic mottle virus and its rna.		1973	4584931
east african strains of cowpea aphid-borne mosaic virus.		1973	4701692
the effects of aflatoxin b1 and palmotoxins b0 and g0 on the germination and leaf colour of the cowpea (vigna sinensis).		1973	4768220
structure and infectivity of picornaviral rna encapsidated by cowpea chlorotic mottle virus protein.	poliovirus and mengo virus rna were shown to associate efficiently with cowpea chlorotic mottle virus protein to form pseudovirions. the sedimentation coefficient for the pseudovirions was similar to that of poliovirus, and electron microscope observations showed the mengo pseudovirions to be similar in size to mengo virus. such pseudovirions were infectious and were more resistant to ribonuclease than viral rna; however, under our assay conditions, their infectivity was about equal to that of v ...	1973	4128376
the mechanism of infection of plant protoplasts by viruses.	the process of virus infection of protoplasts isolated from tobacco leaves has been examined by means of electron microscopy. immediately after inoculation, virus particles appear at two types of site: trapped in complex surface lesions of the plasmalemma, or in peripheral cytoplasmic vesicles. the complex lesions are only visible after treatment of the protoplasts with inocula containing poly-l-ornithine. with infection by tobacco mosaic virus and cowpea chlorotic mottle virus, which require po ...	1973	24468812
variation in pathogenicity of seventeen isolates of meloidogyne incognita.	pathogenicity of 17 isolates of meloidogyne incognita collected in tennessee was studied in the greenhouse on: rutgers tomato, n.c. 95 tobacco, mcnair 1032 cotton, dixie queen watermelon, california wonder pepper and line m57-13n cowpea. root-knot indices of the isolates on the different hosts differentiated six physiological races. the host reactions of each race are discussed.	1973	19319300
the tryptic peptides and terminal sequences of the protein from the cowpea strain of tobacco mosaic virus.		1972	4640687
citrus oils as protectants of black-eyed peas against cowpea weevils: laboratory evaluation.		1972	5085809
sexual competition of gamma-sterilized male cowpea weevils.		1971	5122367
a bacterium and dipterous parasite in wild populations of cowpea curculio larvae: effects of treatment with spores of metarrhizium anisopliae.		1971	4930318
antigenic analysis of rhizobium japonicum by immunodiffusion.	immunodiffusion reactions were studied with seven strains of rhizobium japonicum and three strains of the cowpea miscellany by using antisera against eight of the strains. most strains yielded only weak precipitin bands when untreated cell suspensions were used as antigens in the diffusions. ultrasonic disruption or heat treatment of the cells led to stronger bands, and immersion in boiling water for 20 min was used as the standard procedure for preparing these bacteria for immunodiffusion analy ...	1971	4998353
serological relationship of brome mosaic and cowpea chlorotic mottle viruses.		1971	4108620
electron microscopy of the bean and cowpea strains of southern bean mosaic virus within leaf cells.		1970	5430452
structures derived from cowpea chlorotic mottle and brome mosaic virus protein.		1969	5784855
some properties of pea green mottle virus, a member of the cowpea mosaic group, isolated in czechoslovakia.		1969	4106754
rhizobial relationship of the genus phaseolus. i. cross-inoculation performance of different species to r. phaseoli, r. japonicum, and the cowpea organism.		1967	6036898
electron microscopy of leaves infected with sowbane mosaic virus and other small polyhedral viruses.	leaves of chenopodium amaranticolor were infected with sowbane mosaic virus (smv), sectioned and examined by electron microscopy. leaves of brassica pekinensis and c. amaranticolor were infected with turnip yellow mosaic virus and cowpea mosaic virus, respectively, and similarly studied. with all three viruses it was difficult, in sections, to distinguish the small isometric virus particles from ribosomes though sometimes this was possible, especially when the viruses crystallized. pretreatment ...	1967	18614059
bentonite aids virus transmission.	the addition of 0.1-1% bentonite to crude sap of erigeron glaucus containing tomato ringspot virus increased transmission of the virus to bean, but similar amounts of bentonite to the same inoculum decreased transmission to cucumber and cowpea. addition of bentonite to crude inoculum of this virus in cucumber, bean, and cowpea reduced transmission to cucumber, bean, and cowpea. bentonite decreased transmission to cowpea of cucumber mosaic virus from young cucumber but increased transmission from ...	1966	18611479
enzymes of the glyoxylate cycle in rhizobia and nodules of legumes.	the relatively high level of fatty acids in soybean nodules and rhizobia from soybean nodules suggested that the glyoxylate cycle might have a role in nodule metabolism. several species of rhizobia in pure culture were found to have malate synthetase activity when grown on a number of different carbon sources. significant isocitrate lyase activity was induced when oleate, which presumably may act as an acetyl coa precursor, was utilized as the principle carbon source. malate synthetase was activ ...	1966	16656404
the amino acid and nucleotide composition of the bean and cowpea strains of southern bean mosaic virus.		1966	5921641
effects of ethylene on auxin transport.	the effect of ethylene on the uptake, distribution and polar transport of c(14) from indole-3-acetic acid-2-c(14) and naphthalene acetic acid-1-c(14) in tissue sections was studied. test species were cotton (gossypium hirsutum, l.) and cowpea (vigna sinensis, endl.). generally, incubation of tissue or intact plants with ethylene reduced the degree of polar auxin transport. ethylene inhibited the movement of both auxins in stem tissue and iaa in petiole tissue of cotton. the effect of ethylene on ...	1966	16656230
quick serological method of classifying strains of rhizobium japonicum in nodules.	means, ura m. (u.s. department of agriculture, beltsville, md.), herbert w. johnson, and r. a. date. quick serological method of classifying strains of rhizobium japonicum in nodules. j. bacteriol. 87:547-553. 1964.-a new method of classifying strains of rhizobium japonicum serologically by utilizing the contents of nodules as antigens is described. the method differs from standard procedures only in the preparation of antigens, but it is less time-consuming. agglutination reactions of culture a ...	1964	14127569
acquired tolerance of leaves to heat.	when bean, cowpea, cucumber, fig and tobacco leaves were heated 15 to 30 seconds at 50 degrees c, 12 to 48 hours later they tolerated a temperature of 55 degrees c up to three times as long for the same degree of heat injury as did leaves which were not previously heated.	1961	17812919
temperature influence on the rate of passage of cucumber mosaic virus through the epidermis of cowpea leaves.		1958	13544110
some variables affecting the use of cowpea as an assay host for cucumber mosaic virus.		1954	13189819
action of inhibitors on hydrogenase in azotobacter.	the inhibitors usually associated with the activity of the cytochrome oxidase system-cyanide and carbon monoxide-are also effective in reducing the oxidation of h(2) by intact cells of azotobacter vinelandii. the hydrogenase system is more sensitive to co than is the respiratory system. oxidation of a carbon source and of hydrogen by azotobacter cells is inhibited in a quantitatively different manner by the following compounds: sodium azide, hydroxylamine, sodium iodoacetate, and sodium fluoride ...	1943	19873342
bacterial spot of cowpea.		1923	17820798