Publications

mycoplasma hominis surgical wound infection: a case report and discussion.	a patient with mycoplasma hominis infection of a clean surgical wound that contained a prosthetic vascular graft had clinical findings similar to those observed in patients with acute bacterial infections. etiologic diagnosis was delayed because m. hominis is not appreciated as a cause of surgical wound infections and because of its special growth characteristics. confirmation of m. hominis infection requires awareness on the part of the clinician and assistance from an informed microbiologist. ...	1988	3340994
association of coexisting vaginal infections and multiple abusers in female children with genital warts.	all 11 girls less than 12 years of age presenting to the pediatric clinic between 1980 and 1986 with genital warts were evaluated by the child protection team for possible sexual abuse. nine of the 11 girls had condylomata acuminata, and two had verruca vulgaris. ten of the 11 had historical and/or physical evidence other than the warts that confirmed sexual abuse. six girls had coexisting vaginal infections such as bacterial vaginosis and infections due to various pathogens including neisseria ...	1988	3358240
mycoplasma hominis septicemia after heart surgery.		1988	3364454
evidence that chlamydia trachomatis causes seronegative arthritis in women.	chlamydia trachomatis elementary bodies (ebs) were found in synovial membranes or synovial fluid cell deposits from five of 15 women with seronegative mono- or oligoarthritis by means of a fluorescein conjugated anti-chlamydial monoclonal antibody (micro trak; syva). genital tract specimens were taken from only five of the patients, one of whom had intra-articular ebs, but none was chlamydia positive. six of 10 patients tested were hla-b27 positive, and chlamydial igg antibody, measured by micro ...	1988	3365028
isolation of genital mycoplasmas and chlamydia trachomatis in stillborn and neonatal autopsy material.	chlamydia trachomatis and the genital mycoplasmas are significantly prevalent in sexually active women. how these organisms may affect the outcome of pregnancy and the neonate was the principal thrust of this investigation. placenta, liver, and lung tissue were cultured from mycoplasma hominis, ureaplasma urealyticum, chlamydia trachomatis, and aerobic as well as anaerobic bacteria in 432 stillborn and neonatal autopsies. genital mycoplasmas were isolated from 36 cases (8.3%). acute chorioamnion ...	1988	3382330
distinction of species and strains of mycoplasmas (mollicutes) by genomic dna fingerprints with an rrna gene probe.	genomic fingerprints of acholeplasma laidlawii, mycoplasma hominis, and mycoplasma pneumoniae strains were obtained by southern blot hybridization of the digested mycoplasmal dnas with an rrna gene probe. the hybridization patterns revealed genotypic heterogeneity among a. laidlawii and m. hominis strains and a remarkable degree of homogeneity among m. pneumoniae strains isolated from pneumonia patients during a 10-year period. genomic fingerprints with the rrna gene probe can thus serve as indi ...	1988	3384931
hematoma infected with mycoplasma hominis.	although mycoplasmas are commonly isolated in the genitourinary and upper respiratory tracts, they are rarely isolated from other sites. we describe a case of a young woman with trauma to her pelvis and genitourinary tract who had a thigh hematoma infected with mycoplasma hominis. the patient had persistent systemic symptoms that resolved with appropriate antibiotics and surgical drainage. this case as well as other cases of mycoplasma-infected hematomas point out the need to consider mycoplasma ...	1988	3399998
[mycoplasma infections of the kidneys].	from the urine cultures of 1238 patients with renal or suspicious for renal disease mycoplasmas were isolated in 119 (9.6%). in 75 patients the mycoplasmas were typified. in 56 patients m. hominis and in 24 patients ur. urealyticum were isolated. in 52 patients there were data for pyelonephritis, in 46 patients there was no renal infection. in 21 patents there were mycoplasmas and arterial hypertension without sufficient data for pyelonephritis. in the group of patients with pyelonephritis and a ...	1988	3414078
urinary tract symptoms: microbiologic evaluation in rural family practice.	in order to define the etiology of urinary symptoms in rural family practice, this study examines 106 patients (88 women, 18 men) who went to their family physicians in private practice or a resident-faculty practice with genitourinary symptoms. evaluation of each patient included history, physical examination, urinalysis, and urine or cervical cultures for bacteria, mycoplasma, and chlamydia. using agar plate culturing techniques, 37 patients (35 percent) were identified as having significant u ...	1988	3414387
prevalence of lower genital tract infections in pregnancy.	a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ...	1988	3128883
non-occurrence of mycoplasma genitalium in clinical specimens.	five hundred and thirteen clinical specimens, mainly from patients with urogenital inflammations, were examined for ureaplasma urealyticum and mycoplasmas, including cultures for mycoplasma genitalium. the study yielded 95 isolates of ureaplasma urealyticum, 37 isolates of mycoplasma hominis and two isolates of mycoplasma fermentans, but no growth of mycoplasma genitalium was obtained. it was concluded that mycoplasma genitalium is a relatively rare inhabitant of the human urogenital tract in is ...	1988	3132377
comparative in vitro activity of fleroxacin (ro 23-6240) against ureaplasma urealyticum and mycoplasma hominis.	the susceptibility of 67 isolated ureaplasma urealyticum, 25 mycoplasma hominis and five mycoplasma spp. reference strains to fleroxacin, ofloxacin, josamycin and doxycycline was determined by agar dilution. josamycin had the best activity against ureaplasma urealyticum, followed in order by doxycycline, ofloxacin and fleroxacin. all the strains were inhibited by josamycin at 0.4 mg/l, while only 38.8% of the strains were inhibited by doxycycline at this concentration. mycoplasma hominis strains ...	1988	3132382
correlation of ultrasonography and bacteriology of the endocervix and posterior cul-de-sac of patients with severe pelvic inflammatory disease.	we evaluated 30 patients admitted with a diagnosis of pelvic inflammatory disease (pid) by culdocentesis and ultrasonography to determine the severity of disease in relation to endocervical and culdocentesis bacteriology. specimens from the endocervix were cultured for neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum. in addition, culdocentesis fluid was cultured for aerobes and anaerobes. patients with n. gonorrhoeae or c. trachomatis had significantl ...	1988	3135606
lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology.	we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ...	1988	3135608
[in vitro activity of new quinolones against mycoplasma pathogenic to humans].	the in vitro activity of new quinolones was evaluated against mycoplasma pneumoniae (10 strains) and mycoplasma hominis (approximately equal to 70 strains) by agar dilution, and against ureaplasma urealyticum (approximately equal to 115 strains) by broth dilution. the static effect of pefloxacin, ofloxacin, ciprofloxacin, enoxacin was investigated for all the strains. rosoxacin was included in the tests for u. urealyticum and m. hominis. pefloxacin, ofloxacin, ciprofloxacin and enoxacin were wit ...	1988	3136430
microbial aetiology and diagnostic criteria of postpartum endometritis in nairobi, kenya.	using a protected triple lumen device, neisseria gonorrhoeae or chlamydia trachomatis, or both, were isolated from the endometriums of five out of 35 women with clinical postpartum endometritis compared with none of a control group of 30 puerperal women without endometritis (p less than 0.05) in nairobi, kenya. these sexually transmitted agents were also found in 12 cervical specimens from women with and three without postpartum endometritis (p = 0.04). mycoplasma hominis and ureaplasma urealyti ...	1988	3137152
[isolation of human urogenital mycoplasma, mycoplasma hominis and ureaplasma urealyticum--comparative study between mycotrim-gu and conventional culture methods].		1988	3137284
gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra.	in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ...	1988	3140532
presence of anaplerotic reactions and transamination, and the absence of the tricarboxylic acid cycle in mollicutes.	cell extracts of the fermentative mollicutes acholeplasma laidlawii b-pg9, acholeplasma morum s2, mycoplasma capricolum 14, mycoplasma gallisepticum s6, mycoplasma pneumoniae fh, mycoplasma hyopneumoniae j and m. genitalium g-37, and the non-fermentative mycoplasma hominis pg-21, mycoplasma hominis 1620 and mycoplasma bovigenitalium pg-11 were examined for 39 cytoplasmic enzyme activities associated with the tricarboxylic acid (tca) cycle, transamination, anaplerotic reactions and other enzyme a ...	1988	3141576
in vitro activity of florphenicol.	florphenicol was active at a lower concentration than chloramphenicol against over half of 234 recent clinical bacterial isolates. the majority (98%) of the isolates were inhibited by florphenicol at a concentration of 8 mg/l or less. florphenicol was particularly effective against chloramphenicol resistant strains of haemophilus influenzae. klebsiella aerogenes and bacteroides spp. florphenicol was bacteristatic for salmonellae and escherichia coli but bactericidal for haemophilus influenzae. f ...	1988	3143587
[a case of peritonitis caused by mycoplasma hominis].		1988	3143792
microbiological profile of the cervix in 1,000 sexually active women.	one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ...	1988	3148302
effects of antimicrobial therapy on sperm-mucus interaction.	sperm-mucus interaction under in-vitro or in-vivo conditions might be affected by microorganisms colonizing the reproductive tract. in order to study the influence of antimicrobial therapy, an extensive microbial screening was performed including chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, a broad spectrum of potentially pathogenic aerobic and anaerobic bacteria, trichomonas vaginalis, herpes simplex virus and yeasts. one-hundred-and-six couples with ...	1988	3182976
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethritis. v. sensitivity of u. urealyticum to selected chemotherapeutic agents].		1988	3210836
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethritis. vi. sensitivity of m. hominis to selected chemotherapeutic agents].		1988	3210837
[genital infections and the course of pregnancy: a prospective study].	the reported study investigates the relationship of genital infections, pathobiochemical findings and demographic data to preterm labor, premature rupture of membranes (prom) and premature delivery. the predictability of chorioamnionitis, puerperal and neonatal infections by these parameters was evaluated concurrently. 301 patients were included in this study between july 1985 and june 1986. 147 of these patients were studied longitudinally during pregnancy, delivery and puerperium (longitudinal ...	1988	3215443
effect of blood culture media on the in vitro recovery of mycoplasma hominis.	despite the prevalence of mycoplasma hominis few cases of septicaemia due to this organism have been reported. the ability of various blood culture media to sustain the growth of an inoculum of m. hominis was therefore studied. of the media tested, an 'in-house' hartley's digest broth with 0.1% glucose was the most efficient. the investigation also demonstrated that growth of m. hominis is adversely affected by the concentration of liquoid (sodium polyanetholsulphonate) present in blood culture ...	1988	3216132
mycoplasma hominis meningitis in a premature infant.		1988	3231501
chronic mycoplasma hominis infection complicating severe intraventricular hemorrhage, in a premature neonate.		1988	3231506
ciprofloxacin treatment of chlamydial infections of urogenital tracts of women.	ciprofloxacin was evaluated in chlamydial infections of the urogenital tracts of women treated with a dosage regimen of 500 mg orally twice a day for seven days. of the 40 women evaluated, 30 were infected with chlamydia trachomatis only, two were infected with neisseria gonorrhoeae only, and a further eight had combined gonococcal and chlamydial infections. ten were found to be harbouring chlamydia trachomatis in the urethra as well as the cervix. neisseria gonorrhoeae was eradicated from all p ...	1988	3278970
mycoplasma hominis septic arthritis.	an unusual case is described of extragenital mycoplasma hominis infection presenting as a septic arthritis of the hip joint in a young paraplegic girl. the few reported cases of m. hominis septic arthritis are summarized. based on the current case and these reported cases, the etiology, presentation, diagnosis, and treatment of this infection are discussed.	1988	3281546
septic arthritis due to dual infection with mycoplasma hominis and ureaplasma urealyticum.	acute septic arthritis of a knee and shoulder developed in a 32-year-old renal transplant patient. cultures yielded mycoplasma hominis and at least 1, and possibly 2, strains of ureaplasma urealyticum. doxycycline therapy controlled the symptoms and signs, and the joints became culture negative. on stopping therapy after 7 months, the arthritis recurred and u. urealyticum was again isolated from the shoulder joint. cessation of doxycycline almost 4 years after the initial episode resulted in ano ...	1988	3283360
nongenitourinary infections caused by mycoplasma hominis in adults.	eleven new cases of mycoplasma hominis infection occurring outside the genitourinary tract in adults not in the puerperium were evaluated in relation to the 25 cases previously reported. cases of bacteremia (n = 14) were commonly associated with trauma or manipulation of the genitourinary tract and were often self-limited. wound infections (n = 14) followed surgery by 4-14 days in most cases and may have arisen from contamination from the genitourinary or respiratory tract. joint infections (n = ...	1988	3293162
elevated antibody titers against cytomegalovirus among patients with testicular cancer.	the epidemiology of testicular cancer (tc) has 2 distinguishing features. first, it has an unusual unimodal age-incidence curve which peaks about age 30, and decreases with increasing age. second, risk of the disease has been consistently associated with a high standard of living inasmuch as it is a disease of young white men of generally higher social class in economically developed populations. these features suggest that risk may be related to relatively late age of exposure to common infecti ...	1988	2831159
in vitro comparison of the activity of doxycycline, tetracycline, erythromycin and a new macrolide, cp 62993, against mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum.	the in vitro activities of doxycycline, tetracycline, erythromycin and a new macrolide, cp 62993, against clinical isolates of mycoplasma pneumoniae (n = 3), mycoplasma hominis (n = 64) and ureaplasma urealyticum (n = 33), were compared using an agar dilution method. m. pneumoniae seemed equally susceptible to the two tetracyclines, while the minimum inhibitory concentrations (mics) of doxycycline were one or two dilution steps lower than the mics of tetracycline for most strains of m. hominis a ...	1988	2843982
role of ureaplasma urealyticum and other pathogens in the development of chronic lung disease of prematurity.	a prospective cohort study enrolling 107 infants weighing less than 1250 g was conducted between september 1, 1986, and november 15, 1987 in order to determine the role of microorganisms on the development of chronic lung disease (cld). ureaplasma urealyticum was isolated significantly more frequently from gastric aspirates and nasopharyngeal or endotracheal aspirates from 43 infants developing cld than from 56 who did not (51% vs. 16%; p less than 0.005). infants developing cld, defined by radi ...	1988	2845345
the efficacy and safety of spiramycin in the treatment of nongonococcal urethritis in men.	twenty-five male patients with nongonococcal urethritis including 15 chlamydial infections, were treated with spiramycin for ten days. all but four patients had been treated previously, mostly with tetracyclines. chlamydia trachomatis was cultured in seven patients and was detected in three additional men by immunofluorescent smear. five other patients had antibodies to chlamydia, and one patient yielded a positive culture for ureaplasma urealyticum and mycoplasma hominis. a successful clinical ...	1988	3053571
protein antigens of genital mycoplasmas.	surface structures of the genital mycoplasmas ureaplasma urealyticum and mycoplasma hominis that are important in the human immune response and pathogenesis of disease are relatively poorly defined. in this study, an unusual antigen complex of u. urealyticum consisting of multiple bands forming a "ladder" pattern after electrophoretic separation was noted. it is similar to the variable v-1 surface antigen of mycoplasma pulmonis. data on u. urealyticum are only preliminary, but the ureaplasma ant ...	1988	3055206
mycoplasma hominis in maternal and fetal infections.	although prevalence of m. hominis colonization during pregnancy varies from 12-50%, its role in infections of the mother and newborn infants is unclear. definite correlations exist with chorioamnionitis and amniotic fluid infections, but as it is rarely isolated alone during these infections, its pathogenic role is uncertain. its association with septic abortion is similarly questioned. prevalence and antibody titers to m. hominis increase with increasing parity. transient bacteremia occurs in a ...	1988	3067641
cervical chlamydia trachomatis and mycoplasmal infections in women with abnormal papanicolaou smears.	in a series of 2,346 papanicolaou-stained smears from women undergoing routine gynaecological examination, 39 showed cytomorphological signs of inflammation suggesting chlamydia trachomatis infection (papanicolaou class ii or iii). the 39 smears were studied microbiologically by the direct-immunofluorescence test and cell culture to see whether chlamydial infection correlated with the presence of mycoplasma hominis and ureaplasma urealyticum. the results were compared with the cytological and co ...	1988	3069248
the importance of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis infections in obstetrics and gynaecology.		1988	3075818
influence of microbial colonization on sperm-mucus interaction in vivo and in vitro.	two-hundred-and-thirty-three asymptomatic couples with a mean duration of infertility of 5 years were submitted to postcoital testing (pt) and to sperm penetration meter test (spmt) and simultaneous microbial screening. cervical swabs and semen specimens were collected for culture of mycoplasma hominis, ureaplasma urealyticum, chlamydia trachomatis, neisseria gonorrhoeae, other potentially pathogenic and commensal aerobic and anaerobic bacteria, herpes simplex virus, vaginal swabs for trichomona ...	1987	3114313
sexually transmittable organisms in the urethra of males with and without urethritis.	in 589 males, 169 with and 420 without urethritis, urethral swabs were taken and assessed semiquantitatively for the sexually transmittable infectious agents neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, trichomonas vaginalis and candida species. the organisms were isolated in patients with and without urethritis as follows: n. gonorrhoeae with urethritis 19.5%, without 0.0%; c. trachomatis with urethritis 16.0%, without 2.9%; u. urealyticum (high cfu- ...	1987	3118595
sternotomy infections with mycoplasma hominis.	mycoplasma hominis caused sternal wound infections with mediastinitis in three patients. one infection occurred in a nonimmunocompromised man after coronary artery bypass grafting. the wound did not heal after repeated debridement, closed irrigation of the mediastinum with povidone-iodine solution, and antimycoplasmal chemotherapy; muscle flap grafting was eventually required. two infections occurred in recipients of heart-lung transplants after the isolation of mycoplasma from bronchial secreti ...	1987	3541722
effect on birth weight of erythromycin treatment of pregnant women.	to test the hypothesis that treatment with antibiotics prevents low birth weight, pregnant women whose vaginal cultures contained ureaplasma urealyticum or mycoplasma hominis (or both) and who gave written informed consent were treated with one of the following: identical looking capsules containing 250 mg of either erythromycin estolate or stearate (active against u urealyticum), or 150 mg of clindamycin hydrochloride (active against m hominis), or placebo, four times daily for six weeks in a r ...	1987	3543767
effect of carbohydrates on growth of ureaplasma urealyticum and mycoplasma hominis.	we examined the effect of 31 carbohydrates on the growth of ureaplasma urealyticum and mycoplasma hominis. arbutin and its breakdown product, hydroquinone, inhibited growth of both species; the other substrates did not alter the extent of growth. volatile and nonvolatile end products of carbohydrate metabolism were not detected by gas chromatography.	1987	3793871
mycoplasmas and in vitro fertilization.	semen samples taken from 135 patients attending an in vitro fertilization clinic were shown to be colonized, 53 with ureaplasma urealyticum (39%) and 16 with mycoplasma hominis (12%). an unidentified mycoplasma species was isolated from the sperm of two patients. m. hominis was recovered from all the washed sperm samples taken from colonized semen, whereas washing the sperm eradicated u. urealyticum from 71% of colonized semen. the presence of mycoplasmas in semen made no significant difference ...	1987	3552747
colonization of sexually abused children with genital mycoplasmas.	although sexually abused children are usually evaluated only for the presence of infections with neisseria gonorrhoeae and treponema pallidum, they are also at risk for acquiring other sexually transmitted organisms prevalent in the adult population. accordingly, we examined pharyngeal, anorectal and genital specimens from 50 children who had been sexually abused and from 40 healthy children who served as controls; these specimens were cultured for mycoplasma hominis and ureaplasma urealyticum. ...	1987	3563830
infection with multiple sexually transmitted agents.	a patient named as a contact by an individual with gonorrhea and syphilis was found to be an asymptomatic carrier of seven organisms known to be transmitted sexually. cultures were positive for neisseria gonorrhoeae, chlamydia trachomatis, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and herpes simplex virus. trichomonas vaginalis was detected by a wet preparation and the papanicolaou smear. the cervix was found to have an ulcer on the posterior lip that yielded herpes simp ...	1987	3573062
bacteraemia and pelvic infection in women due to ureaplasma urealyticum and mycoplasma hominis.	during a 22-month period, pelvic infection with bacteraemia that was due to genital mycoplasmas was diagnosed in 12 adult patients at the royal women's hospital. mycoplasma hominis was isolated from seven patients and ureaplasma urealyticum from five patients. infections occurred postpartum in seven patients (in three patients after a vaginal delivery and in four patients after a caesarean section) and after gynaecological procedures in five patients. all patients were moderately ill. during the ...	1987	3574192
[incidence of mycoplasma hominis and ureaplasma urealyticum in pregnant women and gynecologic patients and their treatment with doxycycline].		1987	3587970
[mycoplasmas and pregnancy].	the pathogenic role of mycoplasms during pregnancy remains quite controverted, depending on the studies; for some it has an incidence on prematurity, delayed growth in utero and premature rupture of the membranes. the purpose of this study was, from a population of patients with term delivery, without any specific pathology, to verify the frequency of mothers carrying mycoplasma hominis or ureaplasma, and to determine the possible consequences on the newborn. a linear analysis of the evolution o ...	1987	3589355
prevalence and significance of mycoplasma hominis and ureaplasma urealyticum in the urines of a non-venereal disease population.	ureaplasma urealyticum organisms (ureaplasmas) and mycoplasma hominis organisms (mycoplasmas) were sought in mid-stream urines collected from 200 men and 200 women attending hospital with conditions of a non-venereal nature. in addition, the urines from 100 male and 100 female healthy volunteers were examined. overall, ureaplasmas were isolated four times more often than mycoplasmas. in individuals less than 50 years of age, the organisms were found in about 20% of men and about 40% of women. in ...	1987	3595751
a microbiologic and clinical study of placental inflammation at term.	this study describes the relationships between histologically evident inflammation of the placenta, membranes, and umbilical cords, and correlates these lesions with clinical outcome and with amniotic fluid and amniotic membrane cultures in pregnancies at risk for clinical infection. the overall frequency of inflammation in 123 placentas was 85.45%. there were good interrelationships between inflammatory lesions at various sites. membrane infiltrates of 3+ were seen in 90.9% of intra-amniotic in ...	1987	3601279
[isolation of mycoplasma hominis from the blood in puerperal fever].		1987	3614885
postcesarean wound infection by mycoplasma hominis in a patient with persistent postpartum fever.	mycoplasma hominis was isolated in pure culture from a wound infection following delivery by cesarean section. the importance of recognizing this organism as a potential pathogen of the female genital tract is emphasized. two commercially available isolation systems that allow the recovery of this organism are also described.	1987	3652655
analysis of protein antigens of mycoplasma hominis: detection of polypeptides involved in the human immune response.	immunoblotting of polypeptide antigens after polyacrylamide gel electrophoresis was used to analyze clinical strains of mycoplasma hominis. there was increased antibody titer for polypeptides comprising a wide range of molecular sizes between paired sera obtained from patients with indication of invasive infection. eight of these antigens were shown to be candidate surface antigens. absorption of hyperimmune rabbit serum with intact cells resulted in elimination or reduction in intensity of band ...	1987	3667227
western blot analysis of the human serum response to mycoplasma hominis.	mycoplasma hominis is a human genital pathogen with importance in postpartum pregnancy complications (postpartum fever/endometritis). previous research has suggested that serum antibody levels to m. hominis are important in predicting which groups of women are at risk. m. hominis strain pg21 was examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and western blots to determine which protein antigens would be good candidates for use in serologic tests. western blots o ...	1987	3667228
an improved method for elimination of mycoplasmas from cell cultures.	cell lines infected by different species of mycoplasma (mycoplasma orale, mycoplasma hominis) were decontaminated by co-culture with human blood monocyte (bm)-derived macrophages and pooled human immunoglobulin preparations. co-cultures with bm-derived macrophages or murine peritoneal macrophages (pm) alone were not successful. the phenotype of infected cell lines did not differ from that of uninfected cell lines as revealed by morphological, enzymecytochemical, and immunocytochemical analysis.	1987	3668257
mycoplasma hominis meningitis in a preterm infant.		1987	3668264
the role of mycoplasmas, ureaplasmas and chlamydiae in the genital tract of women presenting in spontaneous early preterm labour.	the genital carriage of ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis was assessed in 72 women admitted to hospital in spontaneous preterm labour and in 26 women requiring preterm delivery for other reasons who formed a control group. women in preterm labour significantly more often carried ureaplasmas, had large numbers of m. hominis and subsequently developed chorioamnionitis than women in the control group. m. hominis, in particular, occurred more frequently and in larg ...	1987	3669058
[biologic properties of ureaplasma urealyticum and mycoplasma hominis isolated from persons with inflammatory conditions of the urethra. i. a method of isolating ureaplasma and mycoplasma from human clinical specimens].		1987	3669803
[biologic properties of ureaplasma urealyticum and mycoplasma hominis isolated from persons with inflammatory conditions of the urethra. ii. occurrence of ureaplasma urealyticum and mycoplasma hominis in healthy men and in those with inflammatory conditions of the urethra and in their sexual partners].		1987	3669804
colonization with genital mycoplasmas in pregnant women and their neonates and birth weight.	the colonization by genital mycoplasmas of mothers and their newborn infants was examined in 114 pregnant women and their 84 neonates. urine and cervical swabs were taken from the pregnant women in the last trimester, and urine from the neonates within six days after birth. ureaplasma urealyticum was found in 73.7% of the pregnant women and in 17.9% of the neonates. mycoplasma hominis was isolated in 8.8% of the material from the pregnant women and in 1.2% of that from the neonates. the isolatio ...	1987	3673336
low birth weight, prematurity, and postpartum endometritis. association with prenatal cervical mycoplasma hominis and chlamydia trachomatis infections.	we studied associations of mycoplasma hominis, ureaplasma urealyticum, and chlamydia trachomatis genital infections with pregnancy outcomes, controlling by logistic and multiple linear regression for known risk factors and for the presence of the other two infections. a sample of 1204 navajo women enrolling for prenatal care had endocervical c trachomatis, m hominis, and u urealyticum cultures and serum samples taken at enrollment and when possible after 30 weeks. low birth weight (less than 250 ...	1987	3806917
chorioamnionitis: its association with pregnancy outcome and microbial infection.	in a study of 33 cases of perinatal death, chorioamnionitis was observed in 57.6% compared with 5% of 20 control cases (p less than 0.001) and in 70.8% of cases with no morphologic cause compared with 22.2% of cases with a defined cause of death (p less than 0.01). chorioamnionitis was significantly associated with previous gestations (p less than 0.01), prolonged rupture of the membranes (p less than 0.001), prematurity (20 to 27 weeks' gestation) (p less than 0.001), and low birth weight (less ...	1987	3826174
measurement of antibody to mycoplasma hominis by an enzyme-linked immunoassay and detection of class-specific antibody responses in women with postpartum fever.	the standard conditions for detection of human igg, igm, and iga antibodies to mycoplasma hominis by an enzyme-linked immunosorbent assay (elisa) were established with the use of a cell lysate antigen and alkaline phosphatase conjugates. antigen was used at a concentration of 10 micrograms of protein per milliliter, sera were diluted 1:200, and conjugates were diluted 1:500. agreement between cultured isolation of m. hominis from the lower genital tract and presence of antibody in 207 women was ...	1987	3826222
relationship of vaginal ph and papanicolaou smear results to vaginal flora and pregnancy outcome.	prematurity is a major cause of perinatal morbidity. studies have implicated components of the vaginal flora in the etiology of some cases of preterm birth. current scoring systems do not include factors which directly reflect the vaginal flora. since papanicolaou smears and the vaginal ph may be affected by the vaginal flora and are easy tests to perform, we studied their relationship to vaginal flora and pregnancy outcome. among 231 patients, those with a vaginal ph greater than or equal to 4. ...	1987	2883041
heterogeneity of mycoplasma hominis as detected by a probe for atp genes.	use of a plasmid containing part of the atp operon of mycoplasma pg50 as a probe in southern blots show that this region can be used to detect the presence of mycoplasma species in general. dna from 14 different strains of m. hominis was analyzed for restriction fragment length polymorphism (rflp) by the use of the same pg50 probe. the m. hominis strains exhibit considerable polymorphism, although some groupings can be observed. these groupings are compared to those obtained for the same strains ...	1987	2889697
prevalence of chlamydia trachomatis and the genital mycoplasmas in a nonmetropolitan population.	results of cultures from patients with nonspecific urethritis (nsu) during a 4-year period were retrospectively analyzed. nsu cultures included chlamydiae and mycoplasmas. seventy-five of 2,408 cultures run by the missoula community hospital microbiology laboratory were positive for chlamydiae (3%). seventy-five of 1,944 mycoplasma cultures were positive for mycoplasma hominis (4%), 532 of 1,944 for ureaplasma urealyticum (27%), and 305 of 1,944, for both (15%), for a total of 46% culture positi ...	1987	2891633
[isolation of mycoplasma and chlamydia in patients with urogenital infections].	vaginal/cervical swabs, catheter urine and/or puncture of the bladder (p.b.) from 880 females with urogenital diseases were examined microbiologically. mycoplasmas (ureaplasma urealyticum, mycoplasma hominis) could be isolated from 321 females (36.5%), and chlamydia trachomatis from 42 (4.8%). mycoplasmas and c. trachomatis were more frequent in the age between 21 and 30 years (53 and 11.8% respectively). in 32.8% of the entire collective mycoplasmas could be detected in vaginal swabs. in contra ...	1987	3301631
electrophoretic analysis of proteins from mycoplasma hominis strains detected by sds-page, two-dimensional gel electrophoresis and immunoblotting.	the proteins of 14 strains of mycoplasma hominis were compared by sds-page in gradient gels, by two-dimensional (2d) gel electrophoresis of extracts of 35s-labelled cells and by immunoblot analysis of cell proteins. the strains examined included the m. hominis type strain pg21 and 13 others isolated variously from genital tract, mouth, blood, upper urinary tract and a wound. these 14 strains shared 76-99% of proteins in sds-gradient gel analysis and 41-72% in the 2d gels. as expected, the immuno ...	1987	3309143
attachment of mycoplasma hominis to human cell cultures.	clinical isolates, cell-culture contaminants, and the type strain pg21 of mycoplasma hominis were examined for attachment to erythrocytes and human cell cultures. strain 13428 (from blood, postpartum fever) and strain 1184 (cell culture) attached to human and guinea pig erythrocytes, but there were no differences in attachment activities between these strains. however, five m. hominis strains isolated from different tissue sites showed quantitative differences in attachment to human widr (intest ...	1987	3312097
dna probes for the detection of mycoplasmas in genital specimens.	the utility of whole-genomic dna probes for the detection of infections by genital mycoplasmas was investigated in 220 men attending a sexually transmitted diseases clinic. in 144 patients, probe results were compared with quantitative culture results. the prevalence of mycoplasma hominis was 11% by culture, whereas the prevalence of ureaplasmas was 38%. the m. hominis dna probe detected 9 of 16 m. hominis culture-positive specimens and 2 of 128 culture-negative specimens. the ureaplasma urealyt ...	1987	3312098
mycoplasma hominis in patients with pelvic inflammatory disease.	the role of mycoplasma hominis in pelvic inflammatory disease (pid) was studied among 35 patients with laparoscopically confirmed salpingitis or histopathologically confirmed plasma cell endometritis. m. hominis was isolated from the cervix of 15 patients, from the rectum of 13 patients, and from the endometrium of 5 patients. only two of these five patients had m. hominis isolated from the endometrium in the absence of chlamydia trachomatis or neisseria gonorrheae, the major pid-causing organis ...	1987	3312109
[detection and identification of mycoplasma infections by dna hybridization].	infection of cell cultures by mycoplasmas can be detected by hybridization of the dna of suspected cell cultures with recombinant plasmids containing fragments of the mycoplasma dna. the test is very sensitive and allows detection of as little as 1 ng of mycoplasmal dna, roughly equivalent to the dna amount of 10(6) mycoplasmas. this approach turns out to be effective for detection and identification of mycoplasmas in clinical material, plant and insect tissues. a set of dna probes for detection ...	1987	3318046
[genital mycoplasma infections--clinical aspects, diagnosis and therapy].	ureaplasma urealyticum und mycoplasma hominis are frequently isolated from the urogenital tract of sexually active persons. their pathogenetic role in urogenital infections and infertility are still controversial. u. urealyticum can cause urethritis and most likely also chronic prostatitis. m. hominis can cause cystitis, salpingitis, and postpartal fever in women and meningitis in newborns. m. genitalium was recently isolated from patients with urethritis and salpingitis. the diagnosis is made b ...	1987	3318082
roxithromycin in nongonococcal urethritis.	this presentation is a summary of five different studies on the efficacy and tolerance of roxithromycin in the treatment of non-gonococcal genital infections. three of the studies were double-blind comparative and two were open studies. of the 924 out-patients whose data were analysed for clinical efficacy, 637 received treatment with roxithromycin 150 mg bd. the standard dose of roxithromycin, 150 mg bd for ten days, was compared with doxycycline 200 mg daily, lymecycline 300 mg bd and roxithro ...	1987	3323168
a review of the in-vitro activity of roxithromycin against genital pathogens.	the in-vitro activity of roxithromycin against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, treponema pallidum, gardnerella vaginalis and haemophilus ducreyi is reviewed. roxithromycin demonstrated equivalent activity to erythromycin against n. gonorrhoeae, c. trachomatis, m. hominis, u. urealyticum, g. vaginalis and h. ducreyi. in a rabbit model for syphilis, potentially useful activity against t. pallidum has been demonstrated.	1987	3323169
[new diagnostic and therapeutic directions in so-called essential anorectal pain].	the authors report a study of 40 patients affected with "essential" ano-rectal neuralgia. it manifests itself by frequent urinary disorders discovered most of the time at the interview of the patient and emphasizes in many cases the presence of psychosomatic disorders. this study has also demonstrated frequent abnormalities of laboratory tests including: chlamydiae serology, urethral samplings and cyto-bacteriological examination of urine looking for: chlamydiae, ureaplasma urealyticum, mycoplas ...	1987	3426144
isolation of genital mycoplasmas from the blood of neonates and women with pelvic infection using conventional sps-free blood culture media.	standard blood culture media used in our laboratory were tested for their ability to support the growth of mycoplasma hominis and ureaplasma urealyticum. small inocula (approximately 10 colony forming units per ml) of both organisms grew in diphasic tryptone soya medium but not in any of several media containing sodium polyanetholesulphonate (sps) including a modified schaedler broth (rwh anaerobic medium) and two bactec media (6b and 7d). both organisms were inhibited even by very low concentra ...	1987	3431914
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethral inflammations. iii. occurrence of serological types of u. urealyticum among the strains isolated from healthy men those with urethral inflammations, and their sexual partners].		1987	3437785
[biological properties of ureaplasma urealyticum and mycoplasma hominis isolated from patients with urethral inflammations. iv. occurrence of serological variants of m. hominis among the strains isolated from healthy men those with urethral inflammations and their sexual partners].		1987	3437786
urinary tract infection (uti) in a couple caused by mycoplasma hominis and ureaplasma urealyticum (a case report).		1987	3449457
a dna probe for detecting mycoplasma genitalium in clinical specimens.	despite decades of careful study, the etiologies of all cases of pelvic inflammatory disease (pid) and non-gonococcal urethritis (ngu) have yet to be described. mycoplasma genitalium is a newly described organism which has been implicated as a cause of both pid and ngu. because of fastidious growth requirements, prolonged incubation time and frequent overgrowth in clinical specimens by mycoplasma hominis, non-culture methods need to be developed for its detection. we have cloned m. genitalium dn ...	1987	3453423
genomic and gene variation in mycoplasma hominis strains.	dnas from 14 strains of mycoplasma hominis isolated from various habitats, including strain pg21, were analyzed for genomic heterogeneity. dna-dna filter hybridization values were from 51 to 91%. restriction endonuclease digestion patterns, analyzed by agarose gel electrophoresis, revealed no identity or cluster formation between strains. variation within m. hominis rrna genes was analyzed by southern hybridization of ecori-cleaved dna hybridized with a cloned fragment of the rrna gene from the ...	1987	2444564
sexually communicable micro-organisms in human semen samples to be used for artificial insemination by donor.	two hundred and thirty seven semen samples from 10 institutes for artificial insemination by donor (aid) in belgium and the netherlands were tested for the presence of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, herpes simplex virus, and cytomegalovirus. the incidence of these micro-organisms in the semen samples was 0%, 6.3%, 4.6%, 35.9%, 0%, and 0.4% respectively, and 47% of all samples were infected with one or more of the micro-organisms. as the ...	1987	3034761
conjugal transfer of transposon tn916 from streptococcus faecalis to mycoplasma hominis.	transposon tn916 was transferred from streptococcus faecalis to mycoplasma hominis by a mating process resembling conjugation with a frequency of 10(-6) to 10(-7). tn916 was inserted into the mycoplasmal chromosome in single and multiple copies.	1987	3038852
[isolation and use of a mycoplasma hominis antigen in the laboratory diagnosis of respiratory diseases].		1987	2471038
[sexually transmitted agents in gynecology: incidence and importance].	the study comprised the analysis of 77 patients between may 1985 and may 1986 in the emergency clinic of the gynecological clinic of the faculty of medicine of the university of sao paulo. three different groups constituted the cases: a) 15 women devoid of infectious symptoms, aged 18-52 with an average of 31.5 years; b) 25 patients who were carriers of mucous purulent cervicitis, aged 16-42 with an average age of 27.6 years; and c) 37 carriers of acute salpingitis, aged 16-48 with an average ...	1987	12288347
prevalence of genital pathogens among female prostitutes in new york city and in rotterdam.	the authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in new york city and 60 female prostitutes attending a sexually transmitted diseases clinic in rotterdam, the netherlands. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in the two cities were 9.3% and 8.3%, 25.3% and 16.6%, 57.3% and 74.9%, and 73% and 79%, respectively. trichomonas vaginalis was detected in 3.6% of new york pr ...	1986	3012805
effect of short term treatment of non-gonococcal urethritis with minocycline.	two hundred and forty four men with non-gonococcal urethritis (ngu) took part in a trial of minocycline. chlamydiae were isolated from 34%, ureaplasmas from 47.1%, and mycoplasma hominis from 10.2%. these micro-organisms were isolated least often from men suffering their third or more attack of ngu, and men in this group failed most often to respond to minocycline. irrespective of the micro-organisms isolated originally or the number of previous attacks, the failure rate (10%) for men receiving ...	1986	3512418
further studies on genital mycoplasmas in intra-amniotic infection: blood cultures and serologic response.	mycoplasma hominis is recovered significantly more often in amniotic fluid of women with intra-amniotic infection than in matched control women, but ureaplasma urealyticum is found in 50% of amniotic fluid samples of both groups. to gain further understanding, we performed blood cultures for genital mycoplasmas and measured serologic responses by a micro enzyme-linked immunosorbent assay method in women with intra-amniotic infection and in control subjects. in blood cultures of 81 women with int ...	1986	3515945
[amine colpitis].	significance of koh test and isonitril (isocyanide) reaction tested in 104 patients was compared with our microbial culture results. in gardnerella vaginalis as well as in no spores forming anaerobic germs the koh test was not and the isonitril reaction was only insignificant reliable. both tests are not able to substitute the procedures of culturing germs including those of mycoplasma hominis and ureaplasma urealyticum.	1986	3532626
aetiology of urinary symptoms in sexually active women.	two hundred and fifty six unselected women, 50 of whom had urinary symptoms (frequency of urination or dysuria, or both), and who were attending a department of genitourinary medicine, were investigated. the urinary symptoms were associated both with pyuria and the isolation of undoubted pathogens from midstream urine (msu) specimens. no associations were found between urinary symptoms and the isolation of neisseria gonorrhoeae or chlamydia trachomatis from the urethra or cervix; the recovery of ...	1986	3533756
[chlamydia trachomatis in patients with genital contact infections in austria--studies of 3,367 patients].	in order to evaluate the epidemiological importance of chlamydia trachomatis (c. trachomatis) as a genital microorganism, data were obtained from 3,367 patients with sexually transmitted diseases in vienna and analyzed by computer-assisted methods. c. trachomatis was cultured in 26.1% of 2,594 patients investigated for the first time. the microorganism was found more often in male patients (31.3%) than in female patients (21%). 32.2% of positive chlamydia cultures were obtained from patients wit ...	1986	3538672
ureaplasma urealyticum as a cause of postpartum fever.	mycoplasma hominis has been shown to cause a postpartum fever in nonimmune women and among the approximately 5% of febrile postpartum women who have the organism isolated from the blood. ureaplasma urealyticum causes postpartum fever in the 20% of febrile women with u. urealyticum alone isolated from the endometrium. about 5% of febrile postpartum women have u. urealyticum isolated from the blood. these data suggest that both genital mycoplasmas can cause postpartum fever.	1986	3540898
a retrospective study of doxycycline in the treatment of genitourinary infections.	a retrospective study was conducted to assess the prevalence of chlamydia trachomatis, ureaplasma ureolyticum, and mycoplasma sp in patients with suspected genital infection, and to assess the efficacy of doxycycline and other current antibiotic therapies. over a three-year period, 1,048 records of patients (64% female; 36% male) were reviewed. c trachomatis, u ureolyticum, or mycoplasma hominis was found in 39% of the men and 49% of the women. other pathogens included gardnerella vaginalis (in ...	1986	3829089
prosthetic joint infection with mycoplasma hominis.		1986	3941284
recovery of microorganisms in semen and relationship to semen evaluation.	semen samples from 120 consecutive unselected men attending our fertility clinic were studied to determine the bacterial isolation rate of semen and its influence on semen quality. each sample was cultured aerobically, anaerobically, and for ureaplasma urealyticum and mycoplasma hominis. the following were analyzed for each semen specimen: motility, morphologic features, and number of sperm cells and viscosity of the ejaculate. four of the 120 samples had negative results; 101 cultures yielded o ...	1986	3943640