Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| anti-biofilm and sporicidal activity of peptides based on wheat puroindoline and barley hordoindoline proteins. | the broad-spectrum activity of antimicrobial peptides (amps) and low probability of development of host resistance make them excellent candidates as novel bio-control agents. a number of amps are found to be cationic, and a small proportion of these are tryptophan-rich. the puroindolines (pin) are small, basic proteins found in wheat grains with proposed roles in biotic defence of seeds and seedlings. synthetic peptides based on their unique tryptophan-rich domain (trd) display antimicrobial pro ... | 2016 | 27238815 |
| arginine patch predicts the rna annealing activity of hfq from gram-negative and gram-positive bacteria. | the sm-protein hfq facilitates interactions between small non-coding rna (srna) and target mrnas. in enteric gram-negative bacteria, hfq is required for srna regulation, and hfq deletion results in stress intolerance and reduced virulence. by contrast, the role of hfq in gram-positive is less established and varies among species. the rna binding and rna annealing activity of hfq from escherichia coli, pseudomonas aeruginosa, listeria monocytogenes, bacillus subtilis, and staphylococcus aureus we ... | 2016 | 27049793 |
| screening of probiotic activities of lactobacilli strains isolated from traditional tibetan qula, a raw yak milk cheese. | in this study, 69 lactobacilli isolated from tibetan qula, a raw yak milk cheese, were screened for their potential use as probiotics. the isolates were tested in terms of: their ability to survive at ph 2.0, ph 3.0, and in the presence of 0.3% bile salts; tolerance of simulated gastric and intestinal juices; antimicrobial activity; sensitivity against 11 specific antibiotics; and their cell surface hydrophobicity. the results show that out of the 69 strains, 29 strains (42%) had survival rates ... | 2016 | 26954218 |
| the listeria monocytogenes fur-regulated virulence protein frva is an fe(ii) efflux p1b4 -type atpase. | listeria monocytogenes frva (lmo0641) is critical for virulence in the mouse model and is an ortholog of the bacillus subtilis fur- and perr-regulated fe(ii) efflux p1b4 -type atpase pfet. previously, frva was suggested to protect against heme toxicity. here, we demonstrate that an frva mutant is sensitive to iron intoxication, but not to other metals. expression of frva is induced by high iron and this induction requires fur. frva functions in vitro as a divalent cation specific atpase most str ... | 2016 | 26946370 |
| an advanced bioinformatics approach for analyzing rna-seq data reveals sigma h-dependent regulation of competence genes in listeria monocytogenes. | alternative σ factors are important transcriptional regulators in bacteria. while σ(b) has been shown to control a large regulon and play important roles in stress response and virulence in the pathogen listeria monocytogenes, the function of σ(h) has not yet been well defined in listeria, even though σ(h) controls a large regulon in the closely related non-pathogenic bacillus subtilis. | 2016 | 26880300 |
| purification and characteristics of a novel bacteriocin produced by enterococcus faecalis l11 isolated from chinese traditional fermented cucumber. | to purify and characterize a novel bacteriocin with broad inhibitory spectrum produced by an isolate of enterococcus faecalis from chinese fermented cucumber. | 2016 | 26875092 |
| identification and characterization of holgh15: the holin of staphylococcus aureus bacteriophage gh15. | holins are phage-encoded hydrophobic membrane proteins that spontaneously and non-specifically accumulate and form lesions in the cytoplasmic membrane. the orf72 gene (also designated holgh15) derived from the genome of the staphylococcus aureus phage gh15 was predicted to encode a membrane protein. an analysis indicated that the protein encoded by holgh15 potentially consisted of two hydrophobic transmembrane helices. this protein exhibited the structural characteristics of class ii holins and ... | 2016 | 26873847 |
| comparison of antimicrobial activities of newly obtained low molecular weight scorpion chitosan and medium molecular weight commercial chitosan. | in this study the antimicrobial activity of low molecular weight (3.22 kda) chitosan, obtained for the first time from a species belonging to the scorpiones, was screened against nine pathogenic microorganisms (seven bacteria and two yeasts) and compared with that of medium molecular weight commercial chitosan (mmwcc). it was observed that the antimicrobial activity of the low molecular weight scorpion chitosan (lmwsc) was specific to bacterial species in general rather than gram-negative or gra ... | 2016 | 26702952 |
| risk assessment in the recovery of food for social solidarity purposes: preliminary data. | the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ... | 2016 | 28058252 |
| differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region. | the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ... | 2016 | 27853715 |
| evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life. | ricotta salata cheese is a salted variety of ricotta traditionally made in sardinia (italy) from the whey remaining after the production of pecorino romano protected designation of origin or other sheep milk cheeses. ricotta salata cheese is very critical for the possible growth of pathogenic and spoilage microorganisms. sporadic cases of listeriosis associated with ricotta salata cheese have been reported over recent years. the objective of the present study was to assess the evolution of spoil ... | 2016 | 27800440 |
| effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review. | according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ... | 2016 | 25612827 |
| antimicrobial activity of kefir against various food pathogens and spoilage bacteria. | kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ... | 2016 | 28115890 |
| a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute. | in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ... | 2016 | 27874136 |
| inactivation of bacterial pathogens in yoba mutandabota, a dairy product fermented with the probiotic lactobacillus rhamnosus yoba. | mutandabota is a dairy product consumed as a major source of proteins and micronutrients in southern africa. in this study the microbial safety of traditional and a variant of mutandabota fermented with the probiotic lactobacillus rhamnosus yoba (yoba mutandabota) was investigated by challenging the products with five important food pathogens: listeria monocytogenes, salmonella spp., campylobacter jejuni, escherichia coli o157:h7 and bacillus cereus. pasteurized full-fat cow's milk was used for ... | 2016 | 26490648 |
| the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria. | goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ... | 2016 | 27698594 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| biological activities of aerial parts extracts of euphorbia characias. | the aim of the present study was to evaluate antioxidant, antimicrobial, anti-hiv, and cholinesterase inhibitory activities of aqueous and alcoholic extracts from leaves, stems, and flowers of euphorbia characias. the extracts showed a high antioxidant activity and were a good source of total polyphenols and flavonoids. ethanolic extracts from leaves and flowers displayed the highest inhibitory activity against acetylcholinesterase and butyrylcholinesterase, showing potential properties against ... | 2016 | 27314007 |
| a rapid multiplex real-time pcr high-resolution melt curve assay for the simultaneous detection of bacillus cereus, listeria monocytogenes, and staphylococcus aureus in food. | three important foodborne pathogens, bacillus cereus, listeria monocytogenes, and staphylococcus aureus, are of great concern for food safety. they may also coexist in food matrices and, in the case of b. cereus and s. aureus, the resulting illnesses can resemble each other owing to similar symptoms. therefore, their simultaneous detection may have advantages in terms of cost savings and rapidity. given this context, a rapid multiplex real-time pcr high-resolution melt curve assay for the simult ... | 2016 | 27296430 |
| assessment of the microbiological quality of meat pies from retail sale in england 2013. | outbreaks of foodborne illness caused by bacillus cereus and listeria monocytogenes in england associated with meat pie consumption were detected in 2012. to obtain baseline data for pies unrelated to outbreaks, 862 samples of ready-to-eat meat pies were collected at retail or from catering facilities in england in 2013 and examined to enumerate food-poisoning bacteria and indicator organisms using organization for standardization (iso) methods for listeria spp. including l. monocytogenes (iso 1 ... | 2016 | 27296425 |
| innovative bionanocomposite films of edible proteins containing liposome-encapsulated nisin and halloysite nanoclay. | films and coatings based on natural polymers have gained increased interest for food packaging applications. in this work, halloysite and phosphatidylcholine liposomes encapsulating nisin were used to develop nanocomposite films of gelatin and casein. liposomes prepared with either soybean lecithin or phospholipon(®) showed particle size ranging from 124 to 178nm and high entrapment efficiency (94-100%). considering their stability, phospholipon(®) liposomes with 1.0mg/ml nisin were selected for ... | 2016 | 27289315 |
| the impact of oxygen on bacterial enteric pathogens. | bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ... | 2016 | 27261784 |
| potential antimicrobial and antiproliferative activities of autochthonous starter cultures and protease epg222 in dry-fermented sausages. | this work studied the presence of nitrogen compounds with bioactive properties in iberian pork sausages that were manufactured using different autochthonous starter cultures (pediococcus acidilactici ms200 and staphylococcus vitulus rs34) and protease epg222. nitrogen compounds were extracted and evaluated for their antimicrobial effect against spoilage and pathogenic bacteria, such as bacillus cereus, escherichia coli, salmonella choleraesuis, staphylococcus aureus and listeria monocytogenes, a ... | 2016 | 27112426 |
| chemical composition, antioxidant and antibacterial activities of essential oils from ferulago angulata. | ferulago angulata boiss. (apiaceae), a perennial aromatic herb, grows wild in iran. the aerial parts of f. angulata are used as a flavouring in foods, especially dairy foods by indigenous people in western and southwestern iran. | 2016 | 27102982 |
| recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph. | the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ... | 2016 | 26976751 |
| application of mathematical models for bacterial inactivation curves using hypericin-based photosensitization. | the aim of this study was to evaluate which of the three mathematical models (weibull, geeraerd and logistic) makes the best approximation for hypericin-based photosensitization bacterial inactivation. | 2016 | 26972682 |
| isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria. | a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ... | 2016 | 26921428 |
| influence of season and type of restaurants on sashimi microbiota. | in recent years, an increase in the consumption of japanese food in european countries has been verified, including in portugal. these specialities made with raw fish, typical japanese meals, have been prepared in typical and on non-typical restaurants, and represent a challenge to risk analysis on haccp plans. the aim of this study was to evaluate the influence of the type of restaurant, season and type of fish used on sashimi microbiota. | 2016 | 26911490 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk. | lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ... | 2016 | 26805974 |
| characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food. | a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ... | 2016 | 26803269 |
| antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens. | the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ... | 2016 | 26775951 |
| advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges. | the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ... | 2016 | 26745757 |
| safety of street-vended soy wara in nigeria. | soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ... | 2016 | 26735046 |
| fapr: from control of membrane lipid homeostasis to a biotechnological tool. | phospholipids and fatty acids are not only one of the major components of cell membranes but also important metabolic intermediates in bacteria. since the fatty acid biosynthetic pathway is essential and energetically expensive, organisms have developed a diversity of homeostatic mechanisms to fine-tune the concentration of lipids at particular levels. fapr is the first global regulator of lipid synthesis discovered in bacteria and is largely conserved in gram-positive organisms including import ... | 2016 | 27766255 |
| isolation and characterization of a broad spectrum bacteriocin from bacillus amyloliquefaciens rx7. | we isolated a bacillus strain, rx7, with inhibitory activity against listeria monocytogenes from soil and identified it as bacillus amyloliquefaciens based on 16s rrna gene sequencing. the inhibitory activity was stable over a wide range of ph and was fully retained after 30 min at 80°c, after which it decreased gradually at higher temperatures. the activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-k, and trypsin, indicating its proteinaceous nature. this bacteriocin ... | 2016 | 27239477 |
| cyclic dipeptide cyclo(l-leucyl-l-prolyl) from marine bacillus amyloliquefaciens mitigates biofilm formation and virulence in listeria monocytogenes. | this study was intentionally focused on cyclo(l-leucyl-l-prolyl) (clp), a cyclic dipeptide with myriad pharmaceutical significance, to explore its antivirulence efficacy against the predominant foodborne pathogen,listeria monocytogenes(lm). minimum inhibitory concentration (mic) of clp against lm atcc 19111 was found to be 512 μg ml(-1) clp at sub-mics (64 128, 256 μg ml(-1)) demonstrated a profound non-bactericidal dose-dependent antibiofilm efficacy (on polystyrene and glass) against lm, which ... | 2016 | 26945590 |
| extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes. | bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ... | 2016 | 27840979 |
| screening and characterization of potential bacillus starter cultures for fermenting low-salt soybean paste (doenjang). | the bacterial strains were screened as potential starters for fermenting low-salt doenjang (a korean traditional fermented soybean paste) using korean doenjang based on proteolytic and antipathogenic activities under 6.5-7.5% nacl conditions. phylogenetic analysis based on 16s rrna gene sequences showed that they all belonged to the genus bacillus. proteolytic and antipathogenic activities against escherichia coli, bacillus cereus, staphylococcus aureus, listeria monocytogenes, and aspergillus f ... | 2016 | 26718466 |
| antimicrobial activity of the pygidial gland secretion of three ground beetle species (insecta: coleoptera: carabidae). | the antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, carabus ullrichii, c. coriaceus, and abax parallelepipedus, have been tested. microdilution method was applied for detection of minimal inhibitory concentrations (mics), minimal bactericidal concentrations (mbcs), and minimal fungicidal concentrations (mfcs). additionally, morpho-histology of the pygidial glands is investigated. we have tested 16 laboratory and clinical strain ... | 2016 | 27000261 |
| molecular characterization and functional analysis of a piscidin gene in large yellow croaker (larimichthys crocea). | the piscidin family, which includes potent antimicrobial peptides with broad-spectrum activity, plays an important role in the innate immune system of fish. in this study, we cloned piscidin-5-like type 3 (lcpis5lt3) in large yellow croaker (larimichthys crocea). multiple alignments with other known piscidins revealed amino acid conservation throughout the fish, especially at the signal peptide (22 amino acids). the phylogenetic tree confirmed that lcpis5lt3 and large yellow croaker piscidin-5-l ... | 2016 | 28105799 |
| effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis). | the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ... | 2016 | 27208583 |
| bacterial diversity of floor drain biofilms and drain waters in a listeria monocytogenes contaminated food processing environment. | sanitation protocols are applied on a daily basis in food processing facilities to prevent the risk of cross-contamination with spoilage organisms. floor drain water serves along with product-associated samples (slicer dust, brine or cheese smear) as an important hygiene indicator in monitoring listeria monocytogenes in food processing facilities. microbial communities of floor drains are representative for each processing area and are influenced to a large degree by food residues, liquid efflue ... | 2016 | 26881738 |
| molecular analysis of dominant species in listeria monocytogenes-positive biofilms in the drains of food processing facilities. | listeria monocytogenes exhibits symbiotic codependence with the dominant commensal bacteria, which may help it avoid being removed or inactivated by disinfectants in local environments. in this study, we investigated l. monocytogenes-positive biofilms at food production facilities, and the dominant bacterial species of the biofilms were identified to determine the properties of the microbiological background. for this purpose, the iso 11290 method was used for the detection and isolation of l. m ... | 2016 | 26658820 |
| fructose 1,6-bisphosphate aldolase, a novel immunogenic surface protein on listeria species. | listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. increased understanding of the surface exposed antigens on listeria would provide potential diagnostic and therapeutic targets. in the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (fba) class ii in listeria species is the antigen target of the previously described mab-3f8. western and ... | 2016 | 27489951 |
| antimicrobial activity of serbian propolis evaluated by means of mic, hptlc, bioautography and chemometrics. | new information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as high performance thin-layer chromatography (hptlc) fingerprinting. this study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of serbian origin against various bacterial species. a linear multivariate calibration technique, using partial least squares, was used t ... | 2016 | 27272728 |
| cip2a promotes t-cell activation and immune response to listeria monocytogenes infection. | the oncoprotein cancerous inhibitor of protein phosphatase 2a (cip2a) is overexpressed in most malignancies and is an obvious candidate target protein for future cancer therapies. however, the physiological importance of cip2a-mediated pp2a inhibition is largely unknown. as pp2a regulates immune responses, we investigated the role of cip2a in normal immune system development and during immune response in vivo. we show that cip2a-deficient mice (cip2ahoz) present a normal immune system developmen ... | 2016 | 27100879 |
| the biofilm lifestyle involves an increase in bacterial membrane saturated fatty acids. | biofilm formation on contact surfaces contributes to persistence of foodborne pathogens all along the food and feed chain. the specific physiological features of bacterial cells embedded in biofilms contribute to their high tolerance to environmental stresses, including the action of antimicrobial compounds. as membrane lipid adaptation is a vital facet of bacterial response when cells are submitted to harsh or unstable conditions, we focused here on membrane fatty acid composition of biofilm ce ... | 2016 | 27840623 |
| normal development and function of t cells in proline rich 7 (prr7) deficient mice. | transmembrane adaptor proteins (traps) are important organisers for the transduction of immunoreceptor-mediated signals. prr7 is a trap that regulates t cell receptor (tcr) signalling and potently induces cell death when overexpressed in human jurkat t cells. whether endogenous prr7 has a similar functional role is currently unknown. to address this issue, we analysed the development and function of the immune system in prr7 knockout mice. we found that loss of prr7 partially impairs development ... | 2016 | 27657535 |
| proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules. | the chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, tenp, ovalbu ... | 2016 | 27294500 |
| licheniocin 50.2 and bacteriocins from lactococcus lactis subsp. lactis biovar. diacetylactis bgbu1-4 inhibit biofilms of coagulase negative staphylococci and listeria monocytogenes clinical isolates. | coagulase negative staphylococci (cons) and listeria monocytogenes have important roles in pathogenesis of various genital tract infections and fatal foetomaternal infections, respectively. the aim of our study was to investigate the inhibitory effects of two novel bacteriocins on biofilms of cons and l. monocytogenes genital isolates. | 2016 | 27930711 |
| comparison of surface proteomes of adherence variants of listeria monocytogenes using lc-ms/ms for identification of potential surface adhesins. | the ability of listeria monocytogenes to adhere and form biofilms leads to persistence in food processing plants and food-associated listeriosis. the role of specific surface proteins as adhesins to attach listeria cells to various contact surfaces has not been well characterized to date. in prior research comparing different methods for surface protein extraction, the ghost urea method revealed cleaner protein content as verified by the least cytoplasmic protein detected in surface extracts usi ... | 2016 | 27196934 |
| antimicrobial activity of croton macrostachyus stem bark extracts against several human pathogenic bacteria. | in kenya, leaves and roots from croton macrostachyus are used as a traditional medicine for infectious diseases such as typhoid and measles, but reports on possible antimicrobial activity of stem bark do not exist. in this study, the antibacterial and antifungal effects of methanol, ethyl acetate and butanol extracts, and purified lupeol of c. macrostachyus stem bark were determined against important human gram-negative pathogens escherichia coli, salmonella typhi, klebsiella pneumoniae, and ent ... | 2016 | 27293897 |
| phenotypes associated with the essential diadenylate cyclase cdaa and its potential regulator cdar in the human pathogen listeria monocytogenes. | cyclic diadenylate monophosphate (c-di-amp) is a second messenger utilized by diverse bacteria. in many species, including the gram-positive human pathogen listeria monocytogenes, c-di-amp is essential for growth. here we show that the single diadenylate cyclase of l. monocytogenes, cdaa, is an integral membrane protein that interacts with its potential regulatory protein, cdar, via the transmembrane protein domain. the presence of the cdar protein is not required for the membrane localization a ... | 2016 | 26527648 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| persistence and potential viable but non-culturable state of pathogenic bacteria during storage of digestates from agricultural biogas plants. | despite the development of on-farm anaerobic digestion as a process for making profitable use of animal by-products, factors leading to the inactivation of pathogenic bacteria during storage of digestates remain poorly described. here, a microcosm approach was used to evaluate the persistence of three pathogenic bacteria (salmonella enterica derby, campylobacter coli and listeria monocytogenes) in digestates from farms, stored for later land spreading. nine samples, including raw digestates, liq ... | 2016 | 27695451 |
| fate of viable but non-culturable listeria monocytogenes in pig manure microcosms. | the fate of two strains of listeria monocytogenes and their ability to become viable but non-culturable (vbnc) was investigated in microcosms containing piggery effluents (two raw manures and two biologically treated manures) stored for 2 months at 8 and 20°c. levels of l. monocytogenes were estimated using the culture method, qpcr, and propidium monoazide treatment combined with qpcr (qpcrpma). the chemical composition and the microbial community structure of the manures were also analyzed. the s ... | 2016 | 26973623 |
| a dual filtration-based multiplex pcr method for simultaneous detection of viable escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on fresh-cut cantaloupe. | fresh-cut cantaloupe is particularly susceptible to contamination with pathogenic bacteria, such as escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus. therefore, development of rapid, yet accurate detection techniques is necessary to ensure food safety. in this study, a multiplex pcr system and propidium monoazide (pma) concentration were optimized to detect all viable pathogens in a single tube. a dual filtration system utilized a filtration membrane with different por ... | 2016 | 27906992 |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| protective effect of carnobacterium spp. against listeria monocytogenes during host cell invasion using in vitro ht29 model. | the pathogenesis of listeriosis results mainly from the ability of listeria monocytogenes to attach, invade, replicate and survive within various cell types in mammalian tissues. in this work, the effect of two bacteriocin-producing carnobacterium (c. divergens v41 and c. maltaromaticum v1) and three non-bacteriocinogenic strains: (c. divergens v41c9, c. divergens 2763, and c. maltaromaticum 2762) was investigated on the reduction of l. monocytogenes scott a plaque-forming during human infection ... | 2016 | 27617232 |
| rapid detection and isolation of escherichia coli o104:h4 from milk using monoclonal antibody-coated magnetic beads. | monoclonal antibodies (mabs) specific for the lipopolysaccharide (lps) of escherichia coli o104:h4 were produced by fusion of sp2/o-ag-14 mouse myeloma cells with spleen cells of balb/c mice, immunized with heat-inactivated and sonicated e. coli o104:h4 bacterial cells. four mabs specific for the e. coli o104:h4 lps (1e6g6, 1f4c9, 3g6g7, and 4g10d2) were characterized and evaluated for the use in a method for the detection of e. coli o104:h4 in milk samples that involves antibody conjugation to ... | 2016 | 27379071 |
| role of host type ia phosphoinositide 3-kinase pathway components in invasin-mediated internalization of yersinia enterocolitica. | many bacterial pathogens subvert mammalian type ia phosphoinositide 3-kinase (pi3k) in order to induce their internalization into host cells. how pi3k promotes internalization is not well understood. also unclear is whether type ia pi3k affects different pathogens through similar or distinct mechanisms. here, we performed an rna interference (rnai)-based screen to identify components of the type ia pi3k pathway involved in invasin-mediated entry of yersinia enterocolitica, an enteropathogen that ... | 2016 | 27068087 |
| comparative resistance of bacterial foodborne pathogens to non-thermal technologies for food preservation. | in this paper the resistance of bacterial foodborne pathogens to manosonication (ms), pulsed electric fields (pefs), high hydrostatic pressure (hhp), and uv-light (uv) is reviewed and compared. the influence of different factors on the resistance of bacterial foodborne pathogens to these technologies is also compared and discussed. only results obtained under harmonized experimental conditions have been considered. this has allowed us to establish meaningful comparisons and draw significant conc ... | 2016 | 27242749 |
| use of lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms. | lactobacillus plantarum is one of the most versatile species extensively used in the food industry both as microbial starters and probiotic microorganisms. several l. plantarum strains have been shown to produce different antimicrobial compounds such as organic acids, hydrogen peroxide, diacetyl, and also bacteriocins and antimicrobial peptides, both denoted by a variable spectrum of action. in recent decades, the selection of microbial molecules and/or bacterial strains able to produce antagoni ... | 2016 | 27148172 |
| biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins. | bacteriophages have been suggested as natural food preservatives as well as rapid detection materials for food-borne pathogens in various foods. since listeria monocytogenes-targeting phage cocktail (listshield) was approved for applications in foods, numerous phages have been screened and experimentally characterized for phage applications in foods. a single phage and phage cocktail treatments to various foods contaminated with food-borne pathogens including e. coli o157:h7, salmonella enterica ... | 2016 | 27092128 |
| cheese microbial risk assessments - a review. | cheese is generally considered a safe and nutritious food, but foodborne illnesses linked to cheese consumption have occurred in many countries. several microbial risk assessments related to listeria monocytogenes, staphylococcus aureus, and escherichia coli infections, causing cheese-related foodborne illnesses, have been conducted. although the assessments of microbial risk in soft and low moisture cheeses such as semi-hard and hard cheeses have been accomplished, it has been more focused on t ... | 2016 | 26950859 |
| cell-based screen identifies human interferon-stimulated regulators of listeria monocytogenes infection. | the type i interferon (ifn) activated transcriptional response is a critical antiviral defense mechanism, yet its role in bacterial pathogenesis remains less well characterized. using an intracellular pathogen listeria monocytogenes (lm) as a model bacterial pathogen, we sought to identify the roles of individual interferon-stimulated genes (isgs) in context of bacterial infection. previously, ifn has been implicated in both restricting and promoting lm growth and immune stimulatory functions in ... | 2016 | 28002492 |
| the listeria monocytogenes pasta kinase prka and its substrate yvck are required for cell wall homeostasis, metabolism, and virulence. | obstacles to bacterial survival and replication in the cytosol of host cells, and the mechanisms used by bacterial pathogens to adapt to this niche are not well understood. listeria monocytogenes is a well-studied gram-positive foodborne pathogen that has evolved to invade and replicate within the host cell cytosol; yet the mechanisms by which it senses and responds to stress to survive in the cytosol are largely unknown. to assess the role of the l. monocytogenes penicillin-binding-protein and ... | 2016 | 27806131 |
| temperature-dependent gentamicin resistance of francisella tularensis is mediated by uptake modulation. | gentamicin (gm) is an aminoglycoside commonly used to treat bacterial infections such as tularemia - the disease caused by francisella tularensis. in addition to being pathogenic, f. tularensis is found in environmental niches such as soil where this bacterium likely encounters gm producers (micromonospora sp.). here we show that f. tularensis exhibits increased resistance to gm at ambient temperature (26°c) compared to mammalian body temperature (37°c). to evaluate whether f. tularensis was les ... | 2016 | 26858709 |
| listeria monocytogenes and the inflammasome: from cytosolic bacteriolysis to tumor immunotherapy. | inflammasomes are cytosolic innate immune surveillance systems that recognize a variety of danger signals, including those from pathogens. listeria monocytogenes is a gram-positive intracellular bacterium evolved to live within the harsh environment of the host cytosol. further, l. monocytogenes can activate a robust cell-mediated immune response that is being harnessed as an immunotherapeutic platform. access to the cytosol is critical for both causing disease and inducing a protective immune r ... | 2016 | 27460808 |
| structural basis of rifampin inactivation by rifampin phosphotransferase. | rifampin (rif) is a first-line drug used for the treatment of tuberculosis and other bacterial infections. various rif resistance mechanisms have been reported, and recently an rif-inactivation enzyme, rif phosphotransferase (rph), was reported to phosphorylate rif at its c21 hydroxyl at the cost of atp. however, the underlying molecular mechanism remained unknown. here, we solve the structures of rph from listeria monocytogenes (lmrph) in different conformations. lmrph comprises three domains: ... | 2016 | 27001859 |
| chemistry and molecular dynamics simulations of heme b-hemq and coproheme-hemq. | recently, a novel pathway for heme b biosynthesis in gram-positive bacteria has been proposed. the final poorly understood step is catalyzed by an enzyme called hemq and includes two decarboxylation reactions leading from coproheme to heme b. coproheme has been suggested to act as both substrate and redox active cofactor in this reaction. in the study presented here, we focus on hemqs from listeria monocytogenes (lmhemq) and staphylococcus aureus (sahemq) recombinantly produced as apoproteins in ... | 2016 | 27599156 |
| exploring the diversity and antimicrobial potential of marine actinobacteria from the comau fjord in northern patagonia, chile. | bioprospecting natural products in marine bacteria from fjord environments are attractive due to their unique geographical features. although, actinobacteria are well known for producing a myriad of bioactive compounds, investigations regarding fjord-derived marine actinobacteria are scarce. in this study, the diversity and biotechnological potential of actinobacteria isolated from marine sediments within the comau fjord, in northern chilean patagonia, were assessed by culture-based approaches. ... | 2016 | 27486455 |
| effects of tween 80 on growth and biofilm formation in laboratory media. | tween 80 is a widely used non-ionic emulsifier that is added to cosmetics, pharmaceuticals, and foods. because of its widespread use we need to understand how it affects bacteria on our skin, in our gut, and in food products. the aim of this study is to investigate how tween 80 affects the growth and antimicrobial susceptibility of staphylococcus aureus, listeria monocytogenes, and pseudomonas fluorescens, which are common causes of spoilage and foodborne illnesses. addition of 0.1% tween 80 to ... | 2016 | 27920774 |
| increased biomass production by mesophilic food-associated bacteria through lowering the growth temperature from 30°c to 10°c. | five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species escherichia coli, listeria monocytogenes, staphylococcus xylosus, bacillus cereus, pedobacter nutrimenti, and pedobacter panaciterrae were tested for the effect of growth temperature (30°c and 10°c) on biomass formation. growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and define ... | 2016 | 27084015 |
| study of the population dynamics of listeria monocytogenes and pseudomonas fluorescens in buffalo mozzarella by means of challenge testing. | campania's buffalo mozzarella is a greatly appreciated cheese in italy and worldwide. from a microbiological standpoint, it is a highly perishable food and potentially at risk of contamination by pathogens such as listeria monocytogenes (l. monocytogenes). the present paper reports the results of a challenge test carried out to assess the population dynamics of l. monocytogenes, alone and in presence of pseudomonas fluorescens (p. fluorescens), in buffalo mozzarella. for this purpose buffalo moz ... | 2016 | 27853707 |
| use of metagenomic shotgun sequencing technology to detect foodborne pathogens within the microbiome of the beef production chain. | foodborne illnesses associated with pathogenic bacteria are a global public health and economic challenge. the diversity of microorganisms (pathogenic and nonpathogenic) that exists within the food and meat industries complicates efforts to understand pathogen ecology. further, little is known about the interaction of pathogens within the microbiome throughout the meat production chain. here, a metagenomic approach and shotgun sequencing technology were used as tools to detect pathogenic bacteri ... | 2016 | 26873315 |
| listeria monocytogenes impact on mature or old pseudomonas fluorescens biofilms during growth at 4 and 20°c. | changes in spatial organization, as observed by confocal laser scanning microscopy (clsm), viable cell content, biovolume, and substratum surface coverage of the biofilms formed on glass by pseudomonas fluorescens resulting from co-culture with listeria monocytogenes, were examined. two strains of l. monocytogenes, two culture temperatures and two biofilm developmental stages were investigated. both l. monocytogenes strains, a persistently sampled isolate (collected repeatedly along 3 years from ... | 2016 | 26913024 |
| inactivation of salmonella spp. and listeria spp. by palmitic, stearic, and oleic acid sophorolipids and thiamine dilauryl sulfate. | food contaminated with human pathogens, such as salmonella spp. and listeria monocytogenes, frequently causes outbreaks of foodborne illness. consumer concern over the use of synthesized antimicrobials to enhance microbial food safety has led to a search of natural alternatives. the objectives of this study were to evaluate the antimicrobial activity of various types of sophorolipids (sls) and thiamine dilauryl sulfate (tds) against pathogenic salmonella spp. and listeria spp. both free and lact ... | 2016 | 28066390 |
| listeria monocytogenes dna glycosylase adlp affects flagellar motility, biofilm formation, virulence, and stress responses. | the temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogen listeria monocytogenes to respond to a changing environment. in this study, a genetic determinant, l. monocytogenes f2365_0220 (lmof2365_0220), encoding a putative protein that is structurally similar to the bacillus cereus alkyl base dna glycosylase (alkd), was identified. this determinant was involved in the transcriptional repression of flagellar motility genes and was named adlp ... | 2016 | 27316964 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) can precisely discriminate the lineages of listeria monocytogenes and species of listeria. | the genetic lineages of listeria monocytogenes and other species of the genus listeria are correlated with pathogenesis in humans. although matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has become a prevailing tool for rapid and reliable microbial identification, the precise discrimination of listeria species and lineages remains a crucial issue in clinical settings and for food safety. in this study, we constructed an accurate and reliable ms databa ... | 2016 | 27442502 |
| thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens. | due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ... | 2016 | 27486447 |
| sublethal concentrations of antibiotics cause shift to anaerobic metabolism in listeria monocytogenes and induce phenotypes linked to antibiotic tolerance. | the human pathogenic bacterium listeria monocytogenes is exposed to antibiotics both during clinical treatment and in its saprophytic lifestyle. as one of the keys to successful treatment is continued antibiotic sensitivity, the purpose of this study was to determine if exposure to sublethal antibiotic concentrations would affect the bacterial physiology and induce antibiotic tolerance. transcriptomic analyses demonstrated that each of the four antibiotics tested caused an antibiotic-specific ge ... | 2016 | 27462313 |
| different transcriptional responses from slow and fast growth rate strains of listeria monocytogenes adapted to low temperature. | listeria monocytogenes has become one of the principal foodborne pathogens worldwide. the capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of l. monocytogenes with different growth capacities at low temperatures. we determined the growth rate at 8°c of 110 strains of l. monocytogenes isolated from different food matrices. we identified a group of slow and fast strains according to thei ... | 2016 | 26973610 |
| evaluation of the performance of quantitative detection of the listeria monocytogenes prfa locus with droplet digital pcr. | fast and reliable pathogen detection is an important issue for human health. since conventional microbiological methods are rather slow, there is growing interest in detection and quantification using molecular methods. the droplet digital polymerase chain reaction (ddpcr) is a relatively new pcr method for absolute and accurate quantification without external standards. using the listeria monocytogenes specific prfa assay, we focused on the questions of whether the assay was directly transferab ... | 2016 | 27558101 |
| chemical defence in a millipede: evaluation and characterization of antimicrobial activity of the defensive secretion from pachyiulus hungaricus (karsch, 1881) (diplopoda, julida, julidae). | the chemical defence of the millipede pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. in total, independently of sex, 44 compounds were identified. all compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. the relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. the two dominant quinones in b ... | 2016 | 27907048 |
| structural basis for glutathione-mediated activation of the virulence regulatory protein prfa in listeria. | infection by the human bacterial pathogen listeria monocytogenes is mainly controlled by the positive regulatory factor a (prfa), a member of the crp/fnr family of transcriptional activators. published data suggest that prfa requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (gsh) could fulfill this function. here we report the crystal structures of prfa in complex with gsh and in complex with gsh and its cognate dna, the hly operator prfa box mot ... | 2016 | 27930316 |
| septins and bacterial infection. | septins, a unique cytoskeletal component associated with cellular membranes, are increasingly recognized as having important roles in host defense against bacterial infection. a role for septins during invasion of listeria monocytogenes into host cells was first proposed in 2002. since then, work has shown that septins assemble in response to a wide variety of invasive bacterial pathogens, and septin assemblies can have different roles during the bacterial infection process. here we review the i ... | 2016 | 27891501 |
| modulation of stat-1 in human macrophages infected with different species of intracellular pathogenic bacteria. | the infection of human macrophages by pathogenic bacteria induces different signaling pathways depending on the type of cellular receptors involved in the microorganism entry and on their mechanism(s) of survival and replication in the host cell. it was reported that stat proteins play an important role in this process. in the present study, we investigate the changes in stat-1 activation (phosphorylation in p-tyr701) after uptake of two gram-positive (listeria monocytogenes and staphylococcus a ... | 2016 | 27437406 |
| spovg is a conserved rna-binding protein that regulates listeria monocytogenes lysozyme resistance, virulence, and swarming motility. | in this study, we sought to characterize the targets of the abundantlisteria monocytogenesnoncoding rna rli31, which is required forl. monocytogeneslysozyme resistance and pathogenesis. whole-genome sequencing of lysozyme-resistant suppressor strains identified loss-of-expression mutations in the promoter ofspovg, and deletion ofspovgrescued lysozyme sensitivity and attenuationin vivoof therli31mutant. spovg was demonstrated to be an rna-binding protein that interacted with rli31in vitro.the rel ... | 2016 | 27048798 |
| response to interferons and antibacterial innate immunity in the absence of tyrosine-phosphorylated stat1. | signal transducer and activator of transcription 1 (stat1) plays a pivotal role in the innate immune system by directing the transcriptional response to interferons (ifns). stat1 is activated by janus kinase (jak)-mediated phosphorylation of y701. to determine whether stat1 contributes to cellular responses without this phosphorylation event, we generated mice with y701 mutated to a phenylalanine (stat1(y701f)). we show that heterozygous mice do not exhibit a dominant-negative phenotype. homozyg ... | 2016 | 26882544 |
| brain abscess following rituximab infusion in a patient with pemphigus vulgaris. | immunocompromised patients are at increased risk for developing meningitis or, rarely, brain abscess with opportunistic organisms like listeria monocytogenes. | 2015 | 25659437 |
| receptor-interacting protein kinase-2 inhibition by cyld impairs antibacterial immune responses in macrophages. | upon infection with intracellular bacteria, nucleotide oligomerization domain protein 2 recognizes bacterial muramyl dipeptide and binds, subsequently, to receptor-interacting serine/threonine kinase 2 (ripk2), which activates immune responses via the nuclear factor kappa-light-chain enhancer of activated b cells (nf-κb) and extracellular signal-regulated kinase (erk) pathways. activation of ripk2 depends on its k63 ubiquitination by e3 ligases, whereas the deubiquitinating enzyme a20 counter re ... | 2015 | 26834734 |
| the legionella kinase legk2 targets the arp2/3 complex to inhibit actin nucleation on phagosomes and allow bacterial evasion of the late endocytic pathway. | legionella pneumophila, the etiological agent of legionellosis, replicates within phagocytic cells. crucial to biogenesis of the replicative vacuole is the dot/icm type 4 secretion system, which translocates a large number of effectors into the host cell cytosol. among them is legk2, a protein kinase that plays a key role in legionella infection. here, we identified the actin nucleator arp2/3 complex as a target of legk2. legk2 phosphorylates the arpc1b and arp3 subunits of the arp2/3 complex. l ... | 2015 | 25944859 |
| avoidance of autophagy mediated by plca or acta is required for listeria monocytogenes growth in macrophages. | listeria monocytogenes is a facultative intracellular pathogen that escapes from phagosomes and grows in the cytosol of infected host cells. most of the determinants that govern its intracellular life cycle are controlled by the transcription factor prfa, including the pore-forming cytolysin listeriolysin o (llo), two phospholipases c (plca and plcb), and acta. we constructed a strain that lacked prfa but expressed llo from a prfa-independent promoter, thereby allowing the bacteria to gain acces ... | 2015 | 25776746 |
| host endoplasmic reticulum copii proteins control cell-to-cell spread of the bacterial pathogen listeria monocytogenes. | listeria monocytogenes is a food-borne pathogen that uses actin-dependent motility to spread between human cells. cell-to-cell spread involves the formation by motile bacteria of plasma membrane-derived structures termed 'protrusions'. in cultured enterocytes, the secreted listeria protein inlc promotes protrusion formation by binding and inhibiting the human scaffolding protein tuba. here we demonstrate that protrusions are controlled by human copii components that direct trafficking from the e ... | 2015 | 25529574 |
| a diverse range of bacterial and eukaryotic chitinases hydrolyzes the lacnac (galβ1-4glcnac) and lacdinac (galnacβ1-4glcnac) motifs found on vertebrate and insect cells. | there is emerging evidence that chitinases have additional functions beyond degrading environmental chitin, such as involvement in innate and acquired immune responses, tissue remodeling, fibrosis, and serving as virulence factors of bacterial pathogens. we have recently shown that both the human chitotriosidase and a chitinase from salmonella enterica serovar typhimurium hydrolyze lacnac from galβ1-4glcnacβ-tetramethylrhodamine (lacnac-tmr (galβ1-4glcnacβ(ch2)8conh(ch2)2nhco-tmr)), a fluorescen ... | 2015 | 25561735 |
| diversity of acid stress resistant variants of listeria monocytogenes and the potential role of ribosomal protein s21 encoded by rpsu. | the dynamic response of microorganisms to environmental conditions depends on the behavior of individual cells within the population. adverse environments can select for stable stress resistant subpopulations. in this study, we aimed to get more insight in the diversity within listeria monocytogenes lo28 populations, and the genetic basis for the increased resistance of stable resistant fractions isolated after acid exposure. phenotypic cluster analysis of 23 variants resulted in three clusters ... | 2015 | 26005439 |
| antagonistic effect of pseudomonas sp. cmi-1 on foodborne pathogenic listeria monocytogenes. | bacterial isolates derived from food or raw food materials of animal origin were screened for potential antagonistic activity against foodborne pathogenic listeria monocytogenes. using the agar spot method, ten out of the 94 tested bacteria showed antilisterial activity. all of the antagonistic isolates identified by sequence analysis as strains of the genus pseudomonas were able to inhibit the growth of all the examined listeria species including the ruminal pathogenic l. ivanovii and the oppor ... | 2015 | 27904352 |