Publications

identification and characterization of a novel marine bacillus cereus for mosquito control.	entomopathogenic bacteria to control mosquitoes are a promising environmentally friendly alternative to synthetic pesticides. in the present study, a novel mosquitocidal bacterium was isolated from marine soil collected from east coastal areas at pondicherry (india). 16s rrna gene sequence alignment depicted that this isolate belonged to bacillus cereus vcrc-b520 (ncbi: kc-119192). biochemical studies on bacterial growth, biomass, and toxin production have revealed that this strain could possibl ...	2014	24192866
fusarial wilt control and growth promotion of pigeon pea through bioactive metabolites produced by two plant growth promoting rhizobacteria.	the bioactive metabolites produced by two plant growth promoting rhizobacteria strains, a pseudomonas aeruginosa strain rrlj 04 and a bacillus cereus strain bs 03, which showed growth promotion and disease control in pigeon pea against fusarium udum, were isolated and screened for their efficacy to control fusarial wilt of pigeon pea under gnotobiotic and nursery condition. bioactive metabolites viz., bm 1 and bm 2 from rrlj 04 and bm 3 from bs 03 also showed in vitro antibiosis against f. udum. ...	2014	24154979
development of new active packaging film made from a soluble soybean polysaccharide incorporated zataria multiflora boiss and mentha pulegium essential oils.	an active edible film from soluble soybean polysaccharide (ssps) incorporated with different concentrations of zataria multiflora boiss (zeo) and mentha pulegium (meo) essential oils was developed, and the film's optical, wettability, thermal, total phenol and antioxidant characteristics were investigated, along with their antimicrobial effectiveness against staphylococcus aureus, bacillus cereus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium. the film's colour beca ...	2014	24176389
potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma.	atmospheric pressure non-thermal plasma (apntp) has been gaining increasing interest as a new alternative antibacterial approach. although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. mechanistic elucidation of the antimicrobial activity will facilitate development and rational optimisation of this approach for potential medical applications. in this study, the antibacterial efficacy of an in-house-built apntp jet was evaluated a ...	2014	24139886
chemical composition and bioactivity of essential oils of hypericum helianthemoides, hypericum perforatum and hypericum scabrum.	a number hypericum species are well known for their therapeutic efficacy and use in traditional medicine. the various species of hypericum have been traditionally used for the treatment of wounds, eczema, burns, trauma, rheumatism, neuralgia, gastroenteritis, ulcers, hysteria, bedwetting and depression.	2014	24073965
optimization of fermentation conditions for green pigment production from bacillus cereus m¹ 16 (mtcc 5521) and its pharmacological application.	optimal culture conditions for the production of green pigment was investigated. the optimal culture condition for the production of an extracellular green pigment by growing bacillus cereus m(1) 16 (mtcc 5521) in a complex medium containing (g l(-1) ) peptone-4.0, beef extract-9.0, nacl-7.0, mgso4 .7h2 o-1.0 and kh2 po4 -5.0 was as follows ph-7.0 at 30°c for 72 h in a 5 l fermenter. aeration rate and agitator speed had no effect on the pigment production. thin layer chromatogram of the pigment ...	2014	23992047
computational analysis of trappc9: candidate gene for autosomal recessive non-syndromic mental retardation.	mental retardation (mr)/ intellectual disability (id) is a neuro-developmental disorder characterized by a low intellectual quotient (iq) and deficits in adaptive behavior related to everyday life tasks such as delayed language acquisition, social skills or self-help skills with onset before age 18. to date, a few genes (prss12, crbn, cc2d1a, grik2, tusc3, trappc9, tecr, st3gal3, med23, man1b1, nsun1) for autosomal-recessive forms of non syndromic mr (ns-armr) have been identified and establishe ...	2014	24040793
the diversity and antimicrobial activity of preussia sp. endophytes isolated from australian dry rainforests.	limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of preussia within australia. this report describes endophytic preussia species that were identified through molecular analysis of the internal transcribed spacer region. screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (etoac) extracts derived from fungal mycelia against a panel of atcc type strains which included bacillus cereus, escher ...	2014	23975673
complete ¹h, ¹⁵n, and ¹³c resonance assignments of bacillus cereus metallo-β-lactamase and its complex with the inhibitor r-thiomandelic acid.	β-lactamases inactivate β-lactam antibiotics by hydrolysis of their endocyclic β-lactam bond and are a major cause of antibiotic resistance in pathogenic bacteria. the zinc dependent metallo-β-lactamase enzymes are of particular concern since they are located on highly transmissible plasmids and have a broad spectrum of activity against almost all β-lactam antibiotics. we present here essentially complete (>96%) backbone and sidechain sequence-specific nmr resonance assignments for the bacillus ...	2014	23838816
microbial toxicity of ethanolamines--multiwalled carbon nanotubes.	in the present study, antimicrobial activities of multiwalled carbon nanotubes (mwcnts) functionalized with ethanolamine (ea) groups were investigated. therefore, mwcnt were first functionalized with mono-, di-, and triethanolamine (mea, dea, and tea) under microwave technique. development of functional groups on the mwcnt surface was confirmed by fourier transform infrared and thermogravimetric analysis. morphological variation was investigated by transmission electron microscopy. then, antimic ...	2014	23784887
identification of cultivable bacteria in the intestinal tract of bactrocera dorsalis from three different populations and determination of their attractive potential.	this study aimed to identify the cultivable bacteria inhabiting the intestinal tract of adult oriental fruit flies (bactrocera dorsalis) from laboratory-reared, laboratory sterile sugar-reared, and field-collected populations, and to evaluate the attractiveness of the metabolites produced by the above bacteria to their hosts.	2014	23696217
enhanced antimicrobial activity of peptide-cocktails against common bacterial contaminants of ex vivo stored platelets.	bacterial contamination of blood components such as ex vivo-stored platelets is a major safety risk in transfusion medicine. we have recently shown that synthetic antimicrobial peptides named pd1-pd4 derived from the thrombin-induced human platelet-derived antimicrobial proteins, and repeats of arg-trp (rw1-rw5) demonstrate microbicidal activity against selected bacteria and viruses. in the present study, we selected pd3, pd4, rw2, rw3 and rw4 and evaluated each individual peptide and their vari ...	2014	23926880
community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees.	cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ...	2014	23893311
antimicrobial activity of amazon astrocaryum aculeatum extracts and its association to oxidative metabolism.	several compounds present in fruits as polyphenols are able to kill or inhibit the growth of microorganisms. these proprieties are relevant mainly in tropical areas, as amazonian region where infectious are highly prevalent. therefore, this study investigated the antimicrobial activity of tucumã amazonian fruit against 37 microorganisms. the potential role of oxidative metabolism imbalance was also studied as causal mechanism of antimicrobial activity. the results showed antibacterial effect of ...	2014	23870852
comparative studies to assess bacterial communities on the clover phylloplane using mlst, dgge and t-rflp.	denaturing gradient gel electrophoresis (dgge) and terminal restriction fragment length polymorphism (t-rflp) were used to characterise the changes that occurred in bacillus cereus group strains present in the phylloplane of clover trifolium hybridum over 4 months. these strains had previously been analysed by multiple locus sequence typing (mlst). dgge displayed many equally intense bands which indicated many equally abundant ribotypes. the bacterial community composition was variable and the l ...	2014	23861044
purification and identification of two antifungal cyclic dipeptides from bacillus cereus subsp. thuringiensis associated with a rhabditid entomopathogenic nematode especially against fusarium oxysporum.	the cell-free culture filtrate of bacillus cereus subsp. thuringiensis associated with an entomopathogenic nematode (epn), rhabditis (oscheius) sp., exhibited strong antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain two cyclic dipeptides (cdps). the structure and absolute stereochemistry of this compound were determined based on extensive spectroscopic analyses (fabms, (1)h nmr, (13)c nmr, (1)h-(1)h cosy ...	2014	23402421
bacillus cereus bacteremia and multiple brain abscesses during acute lymphoblastic leukemia induction therapy.	bacillus cereus can cause serious infections in immunosuppressed patients. this population may be susceptible to b. cereus pneumonia, bacteremia, cellulitis, and rarely cerebral abscess. here we report an 8-year-old boy undergoing induction therapy for acute lymphoblastic leukemia who developed multifocal b. cereus cerebral abscesses, highlighting the propensity for b. cereus to develop cerebral abscesses. a review of the literature over the past 25 years identified another 11 cases (3 children ...	2014	23619116
novel inhibitor of bacterial sphingomyelinase, smy-540, developed based on three-dimensional structure analysis.	bacterial sphingomyelinase (smase) is thought to play a crucial role in bacterial evasion of the immune response during the early stages of infections.	2014	23488740
protein- and dna-based anthrax toxin vaccines confer protection in guinea pigs against inhalational challenge with bacillus cereus g9241.	in the past decade, several bacillus cereus strains have been isolated from otherwise healthy individuals who succumbed to bacterial pneumonia presenting symptoms resembling inhalational anthrax. one strain was indistinguishable from b. cereus g9241, previously cultured from an individual who survived a similar pneumonia-like illness and which was shown to possess a complete set of plasmid-borne anthrax toxin-encoding homologs. the finding that b. cereus g9241 pathogenesis in mice is dependent o ...	2014	25044336
decontamination of a bsl3 laboratory by hydrogen peroxide fumigation using three different surrogates for bacillus anthracis spores.	two independent trials investigated the decontamination of a bsl3 laboratory using vaporous hydrogen peroxide and compared the effect on spores of bacillus cereus, bacillus subtilis and bacillus thuringiensis as surrogates for bacillus anthracis spores, while spores of geobacillus stearothermophilus served as control.	2014	25040253
bacillus anthracis-like bacteria and other b. cereus group members in a microbial community within the international space station: a challenge for rapid and easy molecular detection of virulent b. anthracis.	for some microbial species, such as bacillus anthracis, the etiologic agent of the disease anthrax, correct detection and identification by molecular methods can be problematic. the detection of virulent b. anthracis is challenging due to multiple virulence markers that need to be present in order for b. anthracis to be virulent and its close relationship to bacillus cereus and other members of the b. cereus group. this is especially the case in environments where build-up of bacillus spores can ...	2014	24945323
complete genome sequence of bacillus cereus sensu lato bacteriophage bcp1.	bacillus cereus sensu lato organisms are an ecologically diverse group that includes etiologic agents of food poisoning, periodontal disease, and anthrax. the recently identified bcp1 bacteriophage infects b. cereus sensu lato and is being developed as a therapeutic decontamination agent and diagnostic countermeasure. we announce the complete genome sequence of bcp1.	2014	24926042
the genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon tnces in emetic bacillus weihenstephanensis.	cereulide is a cyclic dodecadepsipeptide ionophore, produced via non-ribosomal peptide synthetases (nrps), which in rare cases can lead to human death. early studies had shown that emetic toxin formation belongs to a homogeneous group of bacillus cereus sensu stricto and the genetic determinants of cereulide (a 24-kb gene cluster of ceshptabcd) are located on a 270-kb plasmid related to the bacillus anthracis virulence plasmid pxo1.	2014	24906385
draft genome sequence of bacillus cereus strain bcfl2013, a clinical isolate similar to g9241.	bacillus cereus strains, such as g9241, causing anthrax-like illnesses have recently been discovered. we report the genome sequence of a clinical strain, b. cereus bcfl2013, which is similar to g9241, recovered from a patient in florida.	2014	24874674
bacillus subtilis systems biology: applications of -omics techniques to the study of endospore formation.	endospore-forming bacteria, with bacillus subtilis being the prevalent model organism, belong to the phylum firmicutes. although the last common ancestor of all firmicutes is likely to have been an endospore-forming species, not every lineage in the phylum has maintained the ability to produce endospores (hereafter, spores). in 1997, the release of the full genome sequence for b. subtilis strain 168 marked the beginning of the genomic era for the study of spore formation (sporulation). in this o ...	2014	26105826
bacillus cereus certhrax adp-ribosylates vinculin to disrupt focal adhesion complexes and cell adhesion.	bacillus cereus is often associated with mild to moderate gastroenteritis; however, some recent isolates cause inhalational anthrax-like diseases and death. these potential emerging human pathogens express multiple virulence factors. b. cereus strain g9241 expresses anthrax toxin, several polysaccharide capsules, and the novel adp-ribosyltransferase, certhrax. in this study, we show that certhrax adp-ribosylates arg-433 of vinculin, a protein that coordinates actin cytoskeleton and extracellular ...	2014	24573681
rapid homogenous time-resolved fluorescence (htrf) immunoassay for anthrax detection.	infection with bacillus anthracsis spores induces an acute anthrax disease that can cause casualties and death in untreated cases. thus rapid diagnosis of anthrax at early stage of the disease is essential to allow an effective treatment. here we present the development of rapid and sensitive homogenous time-resolved fluorescence (htrf) immunoassays based on the energy transfer process of europium cryptate (euk) donor to alexafluor647 acceptor. the energy transfer process is limited to d < 10 nm ...	2014	24515915
inactivation and ultrastructure analysis of bacillus spp. and clostridium perfringens spores.	bacterial endospores are resistant to many environmental factors from temperature extremes to ultraviolet irradiation and are generally more difficult to inactivate or kill than vegetative bacterial cells. it is often considered necessary to treat spores or samples containing spores with chemical fixative solutions for prolonged periods of time (e.g., 1-21 days) to achieve fixation/inactivation to enable electron microscopy (em) examination outside of containment laboratories. prolonged exposure ...	2014	24503289
novel giant siphovirus from bacillus anthracis features unusual genome characteristics.	here we present vb_bans-tsamsa, a novel temperate phage isolated from bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. tsamsa phage is a giant siphovirus (order caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. we induced tsamsa phage in samples from two different carcass sites in etosha national park, namibia. the tsamsa phage genome is th ...	2014	24475065
the regulated synthesis of a bacillus anthracis spore coat protein that affects spore surface properties.	examine the regulation of a spore coat protein and the effects on spore properties.	2014	24460572
exoproteome analysis of a novel strain of bacillus cereus implicated in disease resembling cutaneous anthrax.	bacillus cereus belongs to b. cereus sensu lato group, shared by six other related species including bacillus anthracis. b. anthracis is the causative agent for serious illness affecting a wide range of animals as well as humans and is a category a biological and toxin warfare (btw) agent. recent studies indicate that a bacillus species other than b. anthracis can cause anthrax-like disease and role of anthrax virulence plasmids (pxo1 and pxo2) on the pathogenicity of b. cereus has been document ...	2014	24412723
identification and validation of specific markers of bacillus anthracis spores by proteomics and genomics approaches.	bacillus anthracis is the causative bacteria of anthrax, an acute and often fatal disease in humans. the infectious agent, the spore, represents a real bioterrorism threat and its specific identification is crucial. however, because of the high genomic relatedness within the bacillus cereus group, it is still a real challenge to identify b. anthracis spores confidently. mass spectrometry-based tools represent a powerful approach to the efficient discovery and identification of such protein marke ...	2014	24379445
the designer proline-rich antibacterial peptide a3-apo prevents bacillus anthracis mortality by deactivating bacterial toxins.	proline-rich antibacterial peptides protect experimental animals from bacterial challenge even if they are unable to kill the microorganisms in vitro. their major in vivo modes of action are inhibition of bacterial protein folding and immunostimulation. here we investigated whether the proline-rich antibacterial peptide dimer a3-apo was able to inhibit bacillus cereus enterotoxin production in vitro and restrict the proliferation of lethal toxin-induced bacillus anthracis replication in mouse ma ...	2014	24164262
antibiotic-resistant heterotrophic plate count bacteria and amoeba-resistant bacteria in aquifers of the mooi river, north west province, south africa.	groundwater in the mooi river catchment is prone to mining, agricultural, municipal and septic tank pollution. in this study physico-chemical and microbiological parameters were determined using appropriate methods. bacterial isolates were identified by 16s rrna sequencing (heterotrophic plate count (hpc) bacteria and amoeba-resistant bacteria (arb)) and multiplex polymerase chain reaction (escherichia coli). antibiotic resistance tests were also performed. physico-chemical parameters were gener ...	2014	25473993
chemical composition and biological activity of pulicaria vulgaris essential oil from iran.	the present study investigated the chemical composition of the essential oil (eo) from aerial parts (flowering stage) of pulicaria vulgaris gaertn. by gc-ms. also, the antimicrobial activity of the eo against gram-positive bacteria (bacillus cereus and staphylococcus aureus), gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and fungi (aspergillus niger and candida albicans) was tested. in total, 23 compounds were recognized, accounting for 98.08% of the eo. the main compounds ...	2014	25532299
antibacterial and antifungal activity of holothuria leucospilota isolated from persian gulf and oman sea.	emergence of antimicrobial resistance toward a number of conventional antibiotics has triggered the search for antimicrobial agents from a variety of sources including the marine environment.	2014	25147657
influence of albizia lebbeck saponin and its fractions on in vitro gas production kinetics, rumen methanogenesis, and rumen fermentation characteristics.	the present study was undertaken to investigate the effect of crude seed powder (csp) and gross saponins extract (gse) of seeds of albizia lebbeck on antimicrobial activity by taking two gram-positive (staphylococcus aureus and bacillus cereus), two gram-negative (escherichia coli and salmonella typhi) bacteria, and two fungi species (aspergillus niger and candida butyric) were taken at 25, 50, 100, 250, and 500 µg levels using agar well diffusion method. zone of inhibition was increased with in ...	2014	24977047
influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities.	the results given in the literature are conflicting when considering the relationship between antimicrobial activity and chitosan characteristics. to be able to clarify, we prepared fifteen homogeneous chitosans with different acetylation degrees (da) and molecular weights (mw) by reacetylation of a fully deacetylated chitin under homogeneous conditions. they were tested at different ph values for their antimicrobial activities against four gram-negative bacteria (escherichia coli, pseudomonas a ...	2014	24929684
synthesis, characterization and biological studies of substituted quinozoline-4-(3h)-ones containing diazepine moiety.	the synthesis and characterization of new series of 1,4-benzodiazepine derivatives have been presented. the structures were confirmed by elemental analyses, ir spectral, (1)h nmr spectral and mass spectral data. all the compounds were screened for in vitro antimicrobial and anthelmintic activities. the antibacterial activity was tested against staphylococcus aureus (gram positive), bacillus cereus (gram positive), escherichia coli (gram negative) and pseudomonas aeruginosa (gram negative). the a ...	2014	24438669
volatile analysis and antimicrobial screening of the parasitic plant cuscuta reflexa roxb. from nepal.	the essential oil from the parasitic vine cuscuta reflexa roxb., collected from kirtipur, kathmandu, nepal, was obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry. from a total of 62 peaks, 61 compounds were identified in the oil, accounting for 99.6% of the oil. the majority of the essential oil was dominated by the relatively rare component cis-3-butyl-4-vinylcyclopentane (26.4%). the oil also contained substantial amounts of limonene (5.1%) and (e)-nerolidol (9 ...	2014	24116676
design, synthesis, antimicrobial activity and anti-hiv activity evaluation of novel hybrid quinazoline-triazine derivatives.	a series of novel hybrid quinazoline-triazine derivatives was designed and synthesized from cyanuric chloride and anthranilic acid through sequential reactions, which contain different pharmacophores like quinazoline and substituted diaryl triazine (data) linked with ethylene diamine. all the newly synthesized compounds were characterized by infrared, (1)h-nmr, (13)c-nmr, ms and elemental analysis. further, we evaluated the in vitro anti-hiv activity of the newly synthesized compounds against hi ...	2014	23327639
mangostanaxanthones i and ii, new xanthones from the pericarp of garcinia mangostana.	two new xanthones: mangostanaxanthones i (3) and ii (5) were isolated from the pericarp of garcinia mangostana, along with four known xanthones: 9-hydroxycalabaxanthone (1), parvifolixanthone c (2), α-mangostin (4), and rubraxanthone (6). their structures were elucidated on the basis of ir, uv, 1d, 2d nmr, and ms spectroscopic data, in addition to comparison with literature data. the isolated compounds were evaluated for their antioxidant, antimicrobial, and quorum-sensing inhibitory activities. ...	2014	25128900
antibacterial, antifungal and antioxidant activities of honey collected from timergara (dir, pakistan).	in this study honeys of acacia modesta, prunus persica, zizyphus sativa and isodon rogosus plants were tested against two gram-positive bacterial strains (staphylococcus aureus and bacillus cereus), two gram-negative bacterial strains (klebsilla pneumonia and escherichia coli) and two fungal strains (alternaria alternata and trichoderma harzianum) through agar well diffusion method. the tested honeys showed high antimicrobial activities to the tested bacterial and fungal strains. all the tested ...	2014	24374434
[evaluation of endoscopy assisted vitrectomy for the treatment of severe traumatic eyes with no light perception].	to evaluate the effect of endoscopy assisted pars plana vitrectomy for severe ocular trauma with no light perception.	2014	24841815
microbes associated with freshly prepared juices of citrus and carrots.	fruit juices are popular drinks as they contain antioxidants, vitamins, and minerals that are essential for human being and play important role in the prevention of heart diseases, cancer, and diabetes. they contain essential nutrients which support the growth of acid tolerant bacteria, yeasts, and moulds. in the present study, we have conducted a microbiological examination of freshly prepared juices (sweet lime, orange, and carrot) by serial dilution agar plate technique. a total of 30 juice s ...	2014	26904628
microbial decontamination of red pepper powder by cold plasma.	effects of the microwave-powered cold plasma treatments (cpts) on the inhibition of microorganisms in red pepper powder, including aspergillus flavus and bacillus cereus spores, were investigated. combinations of heat treatment with cpt were investigated for the inhibition of b. cereus spores on the powder. the number of a. flavus was reduced by 2.5 ± 0.3 log spores/g by the cpt with nitrogen at 900 w and 667 pa for 20 min. cpt at 900 w and 667 pa for 20 min inhibited naturally occurring total a ...	2014	24290635
metal based pharmacologically active agents: synthesis, structural characterization, molecular modeling, ct-dna binding studies and in vitro antimicrobial screening of iron(ii) bromosalicylidene amino acid chelates.	in recent years, great interest has been focused on fe(ii) schiff base amino acid complexes as cytotoxic and antitumor drugs. thus a series of new iron(ii) complexes based on schiff bases amino acids ligands have been designed and synthesized from condensation of 5-bromosalicylaldehyde (bs) and α-amino acids (l-alanine (ala), l-phenylalanine (phala), l-aspartic acid (aspa), l-histidine (his) and l-arginine (arg)). the structure of the investigated iron(ii) complexes was elucidated using elementa ...	2014	24001978
cyclic dipeptides from rhabditid entomopathogenic nematode-associated bacillus cereus have antimicrobial activities.	the cell free culture filtrate of bacillus cereus associated with an entomopathogenic nematode, rhabditis (oscheius) sp. exhibited strong antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain four bioactive compounds. the structure and absolute stereochemistry of these compounds were determined based on extensive spectroscopic analyses (fabms, (1)h nmr, (13)c nmr, (1)h-(1)h cosy, (1)h-(13)c hmbc) and marfey' ...	2014	23979826
the biosynthesis of udp-d-fucnac-4n-(2)-oxoglutarate (udp-yelosamine) in bacillus cereus atcc 14579: pat and pyl, an aminotransferase and an atp-dependent grasp protein that ligates 2-oxoglutarate to udp-4-amino-sugars.	surface glycan switching is often observed when micro-organisms transition between different biotic and abiotic niches, including biofilms, although the advantages of this switching to the organism are not well understood. bacillus cereus grown in a biofilm-inducing medium has been shown to synthesize an unusual cell wall polysaccharide composed of the repeating subunit →6)gal(α1-2)(2-r-hydroxyglutar-5-ylamido)fuc2nac4n(α1-6)glcnac(β1→, where galactose is linked to the hydroxyglutarate moiety of ...	2014	25368324
nitropropenyl benzodioxole, an anti-infective agent with action as a protein tyrosine phosphatase inhibitor.	we report on the activities of a broad spectrum antimicrobial compound,nitropropenyl benzodioxole (npbd) which are of relevance to its potential as an anti-infective drug. these investigations support the proposal that a major mechanism of npbd is action as a tyrosine mimetic, competitively inhibiting bacterial and fungal protein tyrosine phosphatases (ptp). npbd did not affect major anti-bacterial drug targets, namely, atp production, cell wall or cell membrane integrity, or transcription and t ...	2014	24976873
novel highly thermostable endolysin from thermus scotoductus mat2119 bacteriophage ph2119 with amino acid sequence similarity to eukaryotic peptidoglycan recognition proteins.	in this study, we present the discovery and characterization of a highly thermostable endolysin from bacteriophage ph2119 infecting thermus strain mat2119 isolated from geothermal areas in iceland. nucleotide sequence analysis of the 16s rrna gene affiliated the strain with the species thermus scotoductus. bioinformatics analysis has allowed identification in the genome of phage 2119 of an open reading frame (468 bp in length) coding for a 155-amino-acid basic protein with an mr of 17,555. ph211 ...	2014	24271162
phages preying on bacillus anthracis, bacillus cereus, and bacillus thuringiensis: past, present and future.	many bacteriophages (phages) have been widely studied due to their major role in virulence evolution of bacterial pathogens. however, less attention has been paid to phages preying on bacteria from the bacillus cereus group and their contribution to the bacterial genetic pool has been disregarded. therefore, this review brings together the main information for the b. cereus group phages, from their discovery to their modern biotechnological applications. a special focus is given to phages infect ...	2014	25010767
identification of pbc218/pbc210 genes of bacillus cereus g9241 in five florida soils using qpcr.	the distribution of the virulent plasmid pbc210 of b. cereus that carries several b. anthracis genes and has been implicated in lethal anthrax-like pulmonary disease is unknown. we screened our collection of 103 b. cereus isolates and 256 soil samples using a quantitative pcr (qpcr) assay that targeted three open reading frames putatively unique to pbc210. when tested with dna from 2 b. cereus strains carrying pbc210, and 64 gram-positive and 55 gram-negative bacterial species, the assay had 100 ...	2014	27350995
the cyclic antibacterial peptide enterocin as-48: isolation, mode of action, and possible food applications.	enterocin as-48 is a circular bacteriocin produced by enterococcus. it contains a 70 amino acid-residue chain circularized by a head-to-tail peptide bond. the conformation of enterocin as-48 is arranged into five alpha-helices with a compact globular structure. enterocin as-48 has a wide inhibitory spectrum on gram-positive bacteria. sensitivity of gram-negative bacteria increases in combination with outer-membrane permeabilizing treatments. eukaryotic cells are bacteriocin-resistant. this catio ...	2014	25493478
antibacterial activity of cinnamaldehyde and clove oil: effect on selected foodborne pathogens in model food systems and watermelon juice.	natural additives for the control of microbial growth are in demand because consumers prefer them over synthetic ones. in the present investigation, the antibacterial activity of two natural preservatives, cinnamaldeyde and clove oil alone or in combinations was studied, and their potential as food preservative in model food systems and watermelon juice was evaluated. the cinnamaldehyde and clove essential oil showed minimum inhibitory concentration (mic) at or below 5000 mg/l, and fractional in ...	2014	26344998
selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting.	the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ...	2014	26139877
a halotolerant thermostable lipase from the marine bacterium oceanobacillus sp. pumb02 with an ability to disrupt bacterial biofilms.	a halotolerant thermostable lipase was purified and characterized from the marine bacterium oceanobacillus sp. pumb02. this lipase displayed a high degree of stability over a wide range of conditions including ph, salinity, and temperature. it was optimally active at 30 °c and ph 8.0 respectively and was stable at higher temperatures (50-70 °c) and alkaline ph. the molecular mass of the lipase was approximately 31 kda based on sds-page and maldi-tof fingerprint analysis. conditions for enhanced ...	2014	25482232
cyclic lipodepsipeptides produced by pseudomonas spp. naturally present in raw milk induce inhibitory effects on microbiological inhibitor assays for antibiotic residue screening.	two pseudomonas strains, identified as closely related to pseudomonas tolaasii, were isolated from milk of a farm with frequent false-positive delvotest results for screening putative antibiotic residues in raw milk executed as part of the regulatory quality programme. growth at 5 to 7°c of these isolates in milk resulted in high lipolysis and the production of bacterial inhibitors. the two main bacterial inhibitors have a molecular weight of 1168.7 and 1140.7 da respectively, are heat-tolerant ...	2014	24853676
the self-assembly of a cyclic lipopeptides mixture secreted by a b. megaterium strain and its implications on activity against a sensitive bacillus species.	cyclic lipopeptides are produced by a soil bacillus megaterium strain and several other bacillus species. in this work, they are detected both in the bacillus intact cells and the cells culture medium by maldi-tof mass spectrometry. the cyclic lipopeptides self-assemble in water media producing negatively charged and large aggregates (300-800 nm of mean hydrodynamic radius) as evaluated by dynamic light scattering and zeta-potential analysis. the aggregate size depends on ph and ionic strength. ...	2014	24816927
the denitrification characteristics of pseudomonas stutzeri sc221-m and its application to water quality control in grass carp aquaculture.	to reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium pseudomonas stutzeri, sc221-m, was obtained. the effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirs and nosz in sc221-m were studied. the following conditions were determined to be optimal for growth and denitrification in sc221-m: nano2 as the nitrogen source, sodium citrate as the ...	2014	25489740
bacillus cereus ar156-induced resistance to colletotrichum acutatum is associated with priming of defense responses in loquat fruit.	the effectiveness of a biocontrol agent bacillus cereus ar156 for control of anthracnose rot caused by colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. treatment of fruit with b. cereus ar156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. the treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxida ...	2014	25386680
a genomic region involved in the formation of adhesin fibers in bacillus cereus biofilms.	bacillus cereus is a bacterial pathogen that is responsible for many recurrent disease outbreaks due to food contamination. spores and biofilms are considered the most important reservoirs of b. cereus in contaminated fresh vegetables and fruits. biofilms are bacterial communities that are difficult to eradicate from biotic and abiotic surfaces because of their stable and extremely strong extracellular matrix. these extracellular matrixes contain exopolysaccharides, proteins, extracellular dna, ...	2014	25628606
identification of multidrug-resistant bacteria and bacillus cereus from healthcare workers and environmental surfaces in a hospital.		2014	25280977
isolation and characterization of broad spectrum coaggregating bacteria from different water systems for potential use in bioaugmentation.	the bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. in this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16s rdna and they were aeromonas, bacillus, comamonas, exiguobacterium, pseudomonas, shewanella and comamonas. furthermore, all possible 231 pa ...	2014	24736645
production of polypeptide antibiotic from streptomyces parvulus and its antibacterial activity.	a highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of visakhapatnam sea coast, bay of bengal. over all ten strains are isolated from the collected soil sediments. among the ten actinomycetes strains the broad spectrum strain rspsn2 was selected for molecular characterization, antibiotic production and its purification. the nucleotide sequence of the 1 rrna gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with stre ...	2014	24948949
identification of 3-sulfinopropionyl coenzyme a (coa) desulfinases within the acyl-coa dehydrogenase superfamily.	in a previous study, the essential role of 3-sulfinopropionyl coenzyme a (3sp-coa) desulfinase acyl-coa dehydrogenase (acd) in advenella mimigardefordensis strain dpn7(t) (acddpn7) during degradation of 3,3'-dithiodipropionic acid (dtdp) was elucidated. dtdp is a sulfur-containing precursor substrate for biosynthesis of polythioesters (ptes). acddpn7 showed high amino acid sequence similarity to acyl-coa dehydrogenases but was unable to catalyze a dehydrogenation reaction. hence, it was investig ...	2014	24317404
alcoholytic cleavage of polyhydroxyalkanoate chains by class iv synthases induced by endogenous and exogenous ethanol.	polyhydroxyalkanoate (pha)-producing bacillus strains express class iv pha synthase, which is composed of the subunits phar and phac. recombinant escherichia coli expressing pha synthase from bacillus cereus strain yb-4 (pharcyb-4) showed an unusual reduction of the molecular weight of pha produced during the stationary phase of growth. nuclear magnetic resonance analysis of the low-molecular-weight pha revealed that its carboxy end structure was capped by ethanol, suggesting that the molecular ...	2014	24334666
diffusible signal factor (dsf) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens.	extensive use of antibiotics has fostered the emergence of superbugs that are resistant to multidrugs, which becomes a great healthcare and public concern. previous studies showed that quorum sensing signal dsf (diffusible signal factor) not only modulates bacterial antibiotic resistance through intraspecies signaling, but also affects bacterial antibiotic tolerance through interspecies communication. these findings motivate us to exploit the possibility of using dsf and its structurally related ...	2014	24575808
antimicrobial and genotoxicity effects of zero-valent iron nanoparticles.	in a world of nanotechnology, the first concern is the potential environmental impact of nanoparticles. an efficient way to estimate nanotoxicity is to monitor the responses of bacteria exposed to these particles.	2014	25147712
iron acquisition in bacillus cereus: the roles of ilsa and bacillibactin in exogenous ferritin iron mobilization.	in host-pathogen interactions, the struggle for iron may have major consequences on the outcome of the disease. to overcome the low solubility and bio-availability of iron, bacteria have evolved multiple systems to acquire iron from various sources such as heme, hemoglobin and ferritin. the molecular basis of iron acquisition from heme and hemoglobin have been extensively studied; however, very little is known about iron acquisition from host ferritin, a 24-mer nanocage protein able to store tho ...	2014	24550730
high antioxidant and dna protection activities of n-acetylglucosamine (glcnac) and chitobiose produced by exolytic chitinase from bacillus cereus ew5.	chitin-degrading bacterial strains were screened and tested for their ability to degrade shrimp-shell waste (ssw). among the potential strains, b. cereus ew5 exhibited the highest chitin-degrading ability compared with other strains and produced 24 mg of reducing sugar per gram of dry ssw after 4 days of incubation. a tlc analysis of ssw biodegradation revealed that the chitosaccharides produced in the culture supernatant were mainly n-acetylglucosamine (glcnac) and chitobiose due to the isolate ...	2014	25077065
clostridium and bacillus binary enterotoxins: bad for the bowels, and eukaryotic being.	some pathogenic spore-forming bacilli employ a binary protein mechanism for intoxicating the intestinal tracts of insects, animals, and humans. these gram-positive bacteria and their toxins include clostridium botulinum (c2 toxin), clostridium difficile (c. difficile toxin or cdt), clostridium perfringens (ι-toxin and binary enterotoxin, or bec), clostridium spiroforme (c. spiroforme toxin or cst), as well as bacillus cereus (vegetative insecticidal protein or vip). these gut-acting proteins for ...	2014	25198129
hygienic-sanitary evaluation of sushi and sashimi sold in messina and catania, italy.	sushi and sashimi are traditional japanese food, mostly consisting of raw seafood alone or in combination with rice. eating sushi and sashimi has become popular in many countries even outside japan. this food is not free from health risks such as ingestion of pathogenic bacteria or parasite. the aim of this study was to investigate on hygienic-sanitary quality of sushi and sashimi sold in the cities of messina and catania, southern italy. fifty samples (38 sushi and 12 sashimi) were analysed to ...	2014	27800343
development of a novel selective and differential medium for the isolation of listeria monocytogenes.	a new medium (lecithin and levofloxacin [ll] medium) is described for the isolation of listeria monocytogenes from food samples. ll medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase c (pi-plc) and phosphatidylcholine-specific phospholipase c (pc-plc) produced by l. monocytogenes. levofloxacin is incorporated to inhibit the growth of microorganisms other than l. monocytogenes, especially bacillus cereus, shown to possess pi-plc and pc-plc acti ...	2014	24271177
draft genome sequences of two bacillus thuringiensis strains and characterization of a putative 41.9-kda insecticidal toxin.	in this work, we report the genome sequencing of two bacillus thuringiensis strains using illumina next-generation sequencing technology (ngs). strain hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (cry) proteins, cry1ia and cry9ea, and a vegetative insecticidal protein (vip) gene, vip3ca2. strain leapi01 contained genes coding for seven cry proteins (cry1aa, cry1ca, cry1da, cry2ab, cry9ea and two cry1ia gene variants) and a vip3 ...	2014	24784323
characterization of a novel manduca sexta beta-1, 3-glucan recognition protein (βgrp3) with multiple functions.	recognition of pathogens by insect pattern recognition receptors is critical to mount effective immune responses. in this study, we reported a new member (βgrp3) of the β-1, 3-glucan recognition protein (βgrp) family from the tobacco hornworm manduca sexta. unlike other members of the m. sexta βgrp family proteins, which contain an n-terminal small glucan binding domain and a c-terminal large glucanase-like domain, βgrp3 is 40-45 residues shorter at the n-terminus and lacks the small glucan bind ...	2014	24952171
studies on antimicrobial and immunomodulatory effects of hot aqueous extract of acacia nilotica l. leaves against common veterinary pathogens.	acacia nilotica is a plant species that is almost ubiquitously found in different parts of the world. various preparations of it have been advocated in folk medicine for the treatment of tuberculosis, leprosy, smallpox, dysentery, cough, ophthalmia, toothache, skin cancer as astringent, antispasmodic, and aphrodisiac since immemorial times. the present study investigates the antibacterial, antifungal, antiviral, and immunomodulatory potential of hot aqueous extract (hae) of acacia nilotica leave ...	2014	24804150
chemical constituents and antimicrobial properties of the essential oil and ethanol extract from the stem of aglaia odorata lour.	the stem-derived essential oil of aglaia odorata lour. was obtained by hydrodistillation using a clevenger-type system. gas chromatography-mass spectrometry analysis of the oil revealed the identification of 39 compounds, representing 76.4% of the oil; germacrene d (20.3%), α-humulene (17.1%), α-himachalene (12.7%) and β-caryophyllene (10.2%) were the major components. ar-turmerone (1) and eichlerialactone (2) were isolated from the stem oil and ethanolic stem extract of this plant species, resp ...	2014	24934340
antimicrobial and anti-inflammatory activities of pleurostylia capensis turcz (loes) (celastraceae).	pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. using ethanol, chloroform, dichloromethane (dcm), ethyl acetate (ea), and water extracts, leaves, bark and roots of pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard method ...	2014	25435632
role of the moth fly clogmia albipunctata (diptera: psychodinae) as a mechanical vector of bacterial pathogens in german hospitals.	the formerly mediterranean moth fly species clogmia albipunctata (diptera: psychodidae) is now present in germany, where it has become a common, year-round pest in hospital buildings.	2013	23182212
mosquitocidal properties of bacillus species isolated from mangroves of vellar estuary, southeast coast of india.	samples collected from the mangroves of vellar estuary yielded a mosquitocidal bacterium, whose secondary metabolites exhibited mosquito larvicidal and pupicidal activity. the bacterium was isolated using standard microbiological methods and identified using classical biochemical tests. the mosquitocidal bacterium was identified as bacillus subtilis, bacillus thuringiensis, bacillus sphaericus and bacillus cereus. mosquitocidal metabolite(s) was separated from the culture supernatant of the bact ...	2013	26345039
antimicrobial activity, growth inhibition of human tumour cell lines, and phytochemical characterization of the hydromethanolic extract obtained from sapindus saponaria l. aerial parts.	the hydromethanolic extract of sapindus saponaria l. aerial parts was investigated for antimicrobial activity (against several gram-positive and gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. the evaluated extract was further characterized in terms of phytochemicals using uv, (1)h-nmr, (13)c-nmr, and ms spectroscopic tools. the extract has shown a significant antimicrobial activity on all tested bacterial and ...	2013	24455713
in vitro characterization of a recombinant ahl-lactonase from bacillus cereus isolated from a striped catfish (pangasianodon hypophthalmus) pond.	aiia gene encoding ahl-lactonase was isolated from bacillus cereus strain n26.2, originating from a striped catfish (pangasianodon hypophthalmus) pond in vietnam. this gene, abbreviated as aiia(n26.2), was cloned and expressed in a competent escherichia coli strain bl21(de3)plyss. the resulting protein, abbreviated as aiian26.2, was highly active in the ph range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °c or for 3 days at 20 °c. these properties of aiian ...	2013	24426155
improving glyphosate oxidation activity of glycine oxidase from bacillus cereus by directed evolution.	glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (go) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the c-n bond in glyphosate. here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a glyphosate-degrading marine strain of bacillus cereus (bcego), we used a bacteriophage ...	2013	24223901
tuning of thioredoxin redox properties by intramolecular hydrogen bonds.	thioredoxin-like proteins contain a characteristic c-x-x-c active site motif and are involved in a large number of biological processes ranging from electron transfer, cellular redox level maintenance, and regulation of cellular processes. the mechanism for deprotonation of the buried c-terminal active site cysteine in thioredoxin, necessary for dissociation of the mixed-disulfide intermediate that occurs under thiol/disulfide mediated electron transfer, is not well understood for all thioredoxi ...	2013	23936007
antimicrobial constituents of the leaves of mikania micrantha h. b. k.	to isolate plant-derived compounds with antimicrobial activity from the leaves of mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (exserohilum turcicum, colletotrichum lagenarium, pseudoperonispora cubensis, botrytis cirerea, rhizoctonia solani, phytophthora parasitica, fusarium solani, and pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: ralstonia dolaanacearum, xan ...	2013	24098556
gram-positive siderophore-shuttle with iron-exchange from fe-siderophore to apo-siderophore by bacillus cereus yxeb.	small molecule iron-chelators, siderophores, are very important in facilitating the acquisition of fe(iii), an essential element for pathogenic bacteria. many gram-negative outer-membrane transporters and gram-positive lipoprotein siderophore-binding proteins have been characterized, and the binding ability of outer-membrane transporters and siderophore-binding proteins for fe-siderophores has been determined. however, there is little information regarding the binding ability of these proteins f ...	2013	23924612
antibacterial activity of 2-alkynoic fatty acids against multidrug-resistant bacteria.	the first study aimed at determining the structural characteristics needed to prepare antibacterial 2-alkynoic fatty acids (2-afas) was accomplished by synthesizing several 2-afas and other analogs in 18-76% overall yields. among all the compounds tested, the 2-hexadecynoic acid (2-hda) displayed the best overall antibacterial activity against gram-positive staphylococcus aureus (mic=15.6 μg/ml), staphylococcus saprophyticus (mic=15.5 μg/ml), and bacillus cereus (mic=31.3 μg/ml), as well as agai ...	2013	24365283
screening of biological activities of polygonum maritimum l. from algerian coast.	to investigate the antioxidant and the antibacterial activities of crude extract from aerial part of polygonum maritimum l. (polygonaceae) (p. maritimum) and to find new actives biomolecules.	2013	23905017
extended genetic analysis of brazilian isolates of bacillus cereus and bacillus thuringiensis.	multiple locus sequence typing (mlst) was undertaken to extend the genetic characterization of 29 isolates of bacillus cereus and bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (vntr). additional analysis involved polymerase chain reaction for the presence of sequences (be, cytk, ina, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. mlst a ...	2013	23440117
endophthalmitis.	endophthalmitis means bacterial or fungal infection inside the eye involving the vitreous and/or aqueous humors. most cases are exogenous and occur after eye surgery, after penetrating ocular trauma, or as an extension of corneal infection. an increasing number of cases are occurring after intravitreal injections of anti-vascular endothelial growth factor (vegf) medications. endophthalmitis may also be endogenous, arising from bacteraemic or fungaemic seeding of the eye. the infected eye never s ...	2013	23438028
the influence of interfering substances on the antimicrobial activity of selected quaternary ammonium compounds.	standard cleaning processes may not remove all the soiling typically found in food industry, such as carbohydrates, fats, or proteins. contaminants have a high impact in disinfection as their presence may reduce the activity of disinfectants. the influence of alginic acid, bovine serum albumin, yeast extract, and humic acids was assessed on the antimicrobial activities of benzalkonium chloride and cetyltrimethyl ammonium bromide against bacillus cereus vegetative cells and pseudomonas fluorescen ...	2013	26904590
inference of the properties of the recombination process from whole bacterial genomes.	patterns of linkage disequilibrium, homoplasy, and incompatibility are difficult to interpret because they depend on several factors, including the recombination process and the population structure. here we introduce a novel model-based framework to infer recombination properties from such summary statistics in bacterial genomes. the underlying model is sequentially markovian so that data can be simulated very efficiently, and we use approximate bayesian computation techniques to infer paramete ...	2013	24172133
differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from burkholderia cepacia and pseudomonas pseudoflava.	the aim of this study is determine the relative sensitivity of a panel of seven polyhydroxyalkanoate producing bacteria to a panel of seven lignocellulosic-derived fermentation inhibitors representing aliphatic acids, furans and phenolics. a further aim was to measure the polyhydroxybutyrate production of select organisms on lignocellulosic-derived monosaccharides arabinose, xylose, glucose and mannose.	2013	23734728
eco-genetic structure of bacillus cereus sensu lato populations from different environments in northeastern poland.	the bacillus cereus group, which includes entomopathogens and etiologic agents of foodborne illness or anthrax, persists in various environments. the basis of their ecological diversification remains largely undescribed. here we present the genetic structure and phylogeny of 273 soil b. cereus s.l. isolates from diverse habitats in northeastern poland, with samplings acquired from the last european natural forest (białowieża national park), the largest marshes in europe (biebrza national park), ...	2013	24312460
root exudate-induced alterations in bacillus cereus cell wall contribute to root colonization and plant growth promotion.	the outcome of an interaction between plant growth promoting rhizobacteria and plants may depend on the chemical composition of root exudates (res). we report the colonization of tobacco, and not groundnut, roots by a non-rhizospheric bacillus cereus (mtcc 430). there was a differential alteration in the cell wall components of b. cereus in response to the res from tobacco and groundnut. attenuated total reflectance infrared spectroscopy revealed a split in amide i region of b. cereus cells expo ...	2013	24205213
functional screening of metagenome and genome libraries for detection of novel flavonoid-modifying enzymes.	the functional detection of novel enzymes other than hydrolases from metagenomes is limited since only a very few reliable screening procedures are available that allow the rapid screening of large clone libraries. for the discovery of flavonoid-modifying enzymes in genome and metagenome clone libraries, we have developed a new screening system based on high-performance thin-layer chromatography (hptlc). this metagenome extract thin-layer chromatography analysis (meta) allows the rapid detection ...	2013	23686272
bacillus cereus cell response upon exposure to acid environment: toward the identification of potential biomarkers.	microorganisms are able to adapt to different environments and evolve rapidly, allowing them to cope with their new environments. such adaptive response and associated protections toward other lethal stresses, is a crucial survival strategy for a wide spectrum of microorganisms, including food spoilage bacteria, pathogens, and organisms used in functional food applications. the growing demand for minimal processed food yields to an increasing use of combination of hurdles or mild preservation fa ...	2013	24106490
exploring the biosynthesis of unsaturated fatty acids in bacillus cereus atcc 14579: functional characterization of novel acyl-lipid desaturases.	at low temperatures, bacillus cereus synthesizes large amounts of unsaturated fatty acids (ufas) with double bonds in positions δ5 and δ10, as well as δ5,10 di-unsaturated fatty acids. through sequence homology searches we identified two orfs encoding a putative δ5 desaturase and a fatty acid acyl-lipid desaturase in b. cereus atcc 14579 genome, named bc2983 and bc0400, respectively. functional characterization by means of heterologous expression in bacillus subtilis of orfs bc2983 and bc0400 co ...	2013	23913431
antioxidative, antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract.	this study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of leea indica leaf ethanol extract. phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, dpph radical scavenging effect, fecl3 reducing power, dmso superoxide scavenging effect and iron chelating effects were studied by established methods. antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine ...	2013	23961238