Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| composition, cytotoxic and antimicrobial activities of satureja intermedia c.a.mey essential oil. | in this study, the essential oil (eo) constituents from the aerial parts of satureja intermedia c.a.mey were detected by gc and gc/ms. the antimicrobial activity of eo on oral pathogens and its cytotoxicity to human cancer cells were determined by the microbroth dilution method and the crystal violet staining method, respectively. thirty-nine compounds were identified and the main eo constituents were γ-terpinene (37.1%), thymol (30.2%), p-cymene (16.2%), limonene (3.9%), α-terpinene (3.3%), myr ... | 2015 | 26247936 |
| antimicrobial activity and chemometric modelling of south african propolis. | this study reports on the inhibitory and bactericidal properties of 39 south african (sa) propolis samples and three propolis samples from brazil. | 2015 | 26189549 |
| antimicrobial activity of silver and gold in toothpastes: a comparative analysis. | in this study, we compared the antimicrobial activity of identical toothpastes differing only in silver or gold nanoparticles against the activity of one of the common toothpastes containing a chemical active ingredient. we also compared the active concentrations of the toothpastes. | 2015 | 26183852 |
| evaluation of antimicrobial and antifungal efficacy of inter appointment intracanal medicaments against enterococcus and candida albicans: an in vitro study. | microorganisms and their by-products in pulpal and periapical diseases are to be considered as the primary etiological agents of the pulpal necrosis and apical periodontitis. enterococcus faecalis, which is the most common organism isolated from failed root canals, is a gram-positive facultative anaerobe. yeasts can be detected in 7-18% of infected root canals. | 2015 | 26124609 |
| a preliminary investigation on the antimicrobial activity of listerine®, its components, and of mixtures thereof. | listerine® is one of the most popular mouthwashes worldwide and claims to combat harmful bacteria. in the past century, its recipe was changed from an essential oil mouthwash to a five-component mixture (thymol, menthol, eucalyptol, and methyl salicylate dissolved in 27% ethanol). the aim of this study was to get preliminary information about the antimicrobial activities of individual listerine® components and their mixtures. we tested the bacterial strains streptococcus mutans, enterococcus fae ... | 2015 | 26104602 |
| antimicrobial and antifungal efficacy of spilanthes acmella as an intracanal medicament in comparison to calcium hydroxide: an in vitro study. | repeated endodontic failures are due to pathogens like enterococcus faecalis and candida albicans which are resistant to the common intracanal medicaments like calcium hydroxide (ca[oh]2). | 2015 | 26672426 |
| pyrrolocin a, a 3-decalinoyltetramic acid with selective biological activity, isolated from amazonian cultures of the novel endophyte diaporthales sp. e6927e. | natural products remain an important source of new therapeutics for emerging drug-resistant pathogens like candida albicans, which particularly affects immunocompromised patients. a bioactive 3-decalinoyltetramic acid, pyrrolocin a, was isolated from extracts of a novel amazonian fungal endophyte, e6927e, of the diaporthales family. the structure of the natural product was solved using nmr and cd spectroscopy and it is structurally related to the fungal setins, equisetin and phomasetin, which ar ... | 2015 | 26669095 |
| assessment of the antimicrobial activity of few saudi arabian snake venoms. | venoms of two cobras, four vipers, a standard antibiotic and an antimycotic, were evaluated comparatively, as antimicrobials. | 2015 | 26668657 |
| utilization of blood cultures in danish hospitals: a population-based descriptive analysis. | this national population-based study was conducted as part of the development of a national automated surveillance system for hospital-acquired bacteraemia and ascertains the utilization of blood cultures (bcs). a primary objective was to understand how local differences may affect interpretation of nationwide surveillance for bacteraemia. from the danish microbiology database, we retrieved all bcs taken between 2010 and 2013 and linked these to admission data from the national patient registry. ... | 2015 | 25658520 |
| antimicrobial and cytotoxic activity of ferula gummosa plant essential oil compared to naocl and chx: a preliminary in vitro study. | the usage of medicinal plants as natural antimicrobial agents has grown in many fields including dental medicine. the aim of this in vitro study was three-fold: (i) to determine the chemical compositions of the ferula gummosa essential oil (fgeo), (ii) to compare the antimicrobial efficacy of the oil with sodium hypochlorite (naocl) and chlorhexidine (chx), (iii) to assess the toxic behavior of fgeo in different concentrations compared to 5% naocl and 0.2% chx. | 2015 | 25671213 |
| adenosine tri-phosphate (atp)-based cleaning monitoring in health care: how rapidly does environmental atp deteriorate? | ensuring cleaning compliance of housekeeping staff is critical to ensure adequate application of surface disinfectants. adenosine triphosphate (atp) testing has been recommended as a way to monitor cleaning compliance; however, little is known about the stability of atp on environmental surfaces. | 2015 | 25794441 |
| the in vitro antimicrobial activity of cymbopogon essential oil (lemon grass) and its interaction with silver ions. | it is well known that cymbopogon (lemon grass) essential oil exhibits antimicrobial activity while the efficacy of silver ions as a disinfectant is equally well reported. | 2015 | 26055131 |
| the efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique. | the aim of this in vitro study was to evaluate the efficacy of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in root canal disinfection by a single-file instrumentation technique. | 2015 | 26007242 |
| antimicrobial effect of zataria multiflora extract in comparison with chlorhexidine mouthwash on experimentally contaminated orthodontic elastomeric ligatures. | long-term use of orthodontic appliances and fixation ligatures creates a favorable environment for the accumulation of oral normal microflora and increases the risk of enamel demineralization and periodontal disease. the aim of this study was to compare the antimicrobial effects of zataria multiflora extract and 0.2% chlorhexidine (chx) mouthwash on experimentally contaminated orthodontic elastomeric ligatures. | 2015 | 26005448 |
| composition and antimicrobial activity of euphrasia rostkoviana hayne essential oil. | eyebright, euphrasia rostkoviana hayne (scrophulariaceae), is a medicinal plant traditionally used in europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. some euphrasia species have been previously reported to contain essential oil. however, the composition and bioactivity of e. rostkoviana oil are unknown. therefore, in this study, we investigated the chemical c ... | 2015 | 26000025 |
| localization of the dual oxidase bli-3 and characterization of its nadph oxidase domain during infection of caenorhabditis elegans. | dual oxidases (duox) are enzymes that contain an nadph oxidase domain that produces hydrogen peroxide (h2o2) and a peroxidase domain that can utilize h2o2 to carry out a variety of reactions. the model organism caenorhabditis elegans produces the duox, bli-3, which has roles in both cuticle development and in protection against infection. in previous work, we demonstrated that while certain peroxidases were protective against the human bacterial pathogen enterococcus faecalis, the peroxidase dom ... | 2015 | 25909649 |
| in vitro antimicrobial and anti-endotoxin action of zingiber officinale as auxiliary chemical and medicament combined to calcium hydroxide and chlorhexidine. | this study was conducted in vitro to compare the effectiveness of zingiber officinale as an auxiliary chemical substance followed by placement of different intra-canal medication in removing endotoxins and cultivable micro-organisms from infected root canals. | 2015 | 25892281 |
| immune response profile against persistent endodontic pathogens candida albicans and enterococcus faecalis in vitro. | persistent microorganisms such as candida albicans and enterococcus faecalis might be directly related to endodontic treatment failure. the host response to these microorganisms impairs the reestablishment of intraradicular and periradicular health. | 2015 | 25845887 |
| performance evaluation of a dna chip assay in the identification of major genitourinary pathogens. | to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections. | 2015 | 25533217 |
| syntheses, structures, and antimicrobial activity of new remarkably light-stable and water-soluble tris(pyrazolyl)methanesulfonate silver(i) derivatives of n-methyl-1,3,5-triaza-7-phosphaadamantane salt - [mpta]bf4. | two new silver(i) complexes of formula [ag(mpta)4](tpms)4(bf4) (1) and [ag(tpms)(mpta)](bf4) (2) (mpta = n-methyl-1,3,5-triaza-7-phosphaadamantane cation, tpms = tris(pyrazol-1-yl)methanesulfonate anion) have been synthesized and fully characterized by elemental analyses, (1)h and (31)p{(1)h} nmr, esi-ms, and ir spectroscopic techniques. the single-crystal x-ray diffraction study of 1 discloses a noncoordinated nature of the tpms species, existing as counterions around the highly charged metal c ... | 2015 | 25531979 |
| microbial community in persistent apical periodontitis: a 16s rrna gene clone library analysis. | to characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. | 2015 | 25088120 |
| evaluation of cytotoxicity, antimicrobial activity and physicochemical properties of a calcium aluminate-based endodontic material. | a calcium aluminate-based endodontic material, endobinder, has been developed in order to reduce mta negative characteristics, preserving its biological properties and clinical applications. | 2015 | 24626250 |
| use of a dissolved oxygen microsensor for assessing the viability and thickness of microbial biofilm on root surfaces. | to evaluate the use of a dissolved oxygen microsensor (doms) for assessing the viability and thickness of microbial biofilms on the apical external surface of contaminated human tooth roots. | 2015 | 24942082 |
| in vitro and ex vivo antimicrobial efficacy of nano-mgo in the elimination of endodontic pathogens. | the use of metal oxide nanoparticles has attracted lots of attention, mostly because of their promising antimicrobial activity along with their biocompatibility with mammalian cells. this study aims to investigate the in vitro and ex vivo antimicrobial efficiency of nano-magnesium oxide (mgo) aqueous solution against endodontic pathogens. | 2015 | 24859291 |
| chronic bacterial seminal vesiculitis as a potential disease entity in men with chronic prostatitis. | to investigate bacterial infection in the seminal vesicles by bacteriological examination and radionuclide imaging in men with chronic prostatitis. | 2015 | 25721352 |
| presence of aerobic micro-organisms and their influence on basic semen parameters in infertile men. | urogenital tract infections in males are one of the significant etiological factors in infertility. in this prospective study, 72 patients with abnormal semen parameters or any other symptoms of urogenital tract infection were examined. semen analysis according to the who 2010 manual was performed together with microbial assessment: aerobic bacteria culture, chlamydia antigen test, candida culture, ureaplasma and mycoplasma-specific culture. in total, 69.4% of semen samples were positive for at ... | 2015 | 25209133 |
| isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ... | 2015 | 27682080 |
| mutations associated with reduced surotomycin susceptibility in clostridium difficile and enterococcus species. | clostridium difficile infection (cdi) is an urgent public health concern causing considerable clinical and economic burdens. cdi can be treated with antibiotics, but recurrence of the disease following successful treatment of the initial episode often occurs. surotomycin is a rapidly bactericidal cyclic lipopeptide antibiotic that is in clinical trials for cdi treatment and that has demonstrated superiority over vancomycin in preventing cdi relapse. surotomycin is a structural analogue of the me ... | 2015 | 25941217 |
| an alkaline phosphatase reporter for use in clostridium difficile. | clostridium difficile is an anaerobic, gram-positive pathogen that causes severe gastrointestinal disease in humans and other mammals. c. difficile is notoriously difficult to work with and, until recently, few tools were available for genetic manipulation and molecular analyses. despite the recent advances in the field, there is no simple or cost-effective technique for measuring gene transcription in c. difficile other than direct transcriptional analyses (e.g., quantitative real-time pcr and ... | 2015 | 25576237 |
| new role for human α-defensin 5 in the fight against hypervirulent clostridium difficile strains. | clostridium difficile infection (cdi), one of the most common hospital-acquired infections, is increasing in incidence and severity with the emergence and diffusion of hypervirulent strains. cdi is precipitated by antibiotic treatment that destroys the equilibrium of the gut microbiota. human α-defensin 5 (hd5), the most abundant enteric antimicrobial peptide, is a key regulator of gut microbiota homeostasis, yet it is still unknown if c. difficile, which successfully evades killing by other hos ... | 2015 | 25547793 |
| effectiveness of probiotic therapy for the prevention of relapse in patients with inactive ulcerative colitis. | to evaluate the effectiveness of probiotic therapy for suppressing relapse in patients with inactive ulcerative colitis (uc). | 2015 | 26019464 |
| polymicrobial q fever and enterococcal aortic prosthetic valve endocarditis with aortic root abscess. | polymicrobial endocarditis is uncommon. we present a case of polymicrobial endocarditis caused by enterococcus faecalis and coxiella burnetii and review previous cases of polymicrobial endocarditis involving coxiella burnetii. testing for q fever should be considered in any patient with endocarditis living in an endemic area. | 2015 | 25988443 |
| a novel method for screening of potential probiotics for high adhesion capability. | to screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. lactobacillus plantarum (hm218749), lactobacillus reuteri (eu547310), and enterococcus faecalis (hm218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°c in an anaerobic environment and could r ... | 2015 | 25912863 |
| early colonizing escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state. | we investigated the effects of early colonizing bacteria on the colonic epithelium. we isolated dominant bacteria, escherichia coli, enterococcus faecalis, lactobacillus intestinalis, clostridium innocuum and a novel fusobacterium spp., from the intestinal contents of conventional suckling rats and transferred them in different combinations into germfree (gf) adult rats. animals were investigated after various times up to 21 days. proliferative cell markers (ki67, proliferating cell nuclear anti ... | 2015 | 25012905 |
| cyclodepsipeptides produced by actinomycetes inhibit cyclic-peptide-mediated quorum sensing in gram-positive bacteria. | cyclic peptides are commonly used as quorum-sensing autoinducers in gram-positive firmicutes bacteria. well-studied examples of such molecules are thiolactone and lactone, used to regulate the expression of a series of virulence genes in the agr system of staphylococcus aureus and the fsr system of enterococcus faecalis, respectively. three cyclodepsipeptides ws9326a, ws9326b and cochinmicin ii/iii were identified as a result of screening actinomycetes culture extracts for activity against the a ... | 2015 | 26149266 |
| synergistic effect of 2% chlorhexidine combined with proteolytic enzymes on biofilm disruption and killing. | to investigate the dynamics of a disinfection regimen using 1% trypsin and 1% proteinase k in combination with 2% chlorhexidine (with or without ultrasonics) using a nutrient-stressed endodontic multispecies model biofilm. | 2015 | 25482847 |
| construction of recombinant pichia pastoris carrying a constitutive avbd9 gene and analysis of its activity. | avian beta-defensin 9 (avbd9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, avbd9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. using the preferred codon of pichia pastoris, the mature avbd9 peptide was designed and synthesized, based on th ... | 2015 | 26370794 |
| effect of in-feed administration and withdrawal of tylosin phosphate on antibiotic resistance in enterococci isolated from feedlot steers. | tylosin phosphate is a macrolide commonly administered to cattle in north america for the control of liver abscesses. this study investigated the effect of in-feed administration of tylosin phosphate to cattle at subtherapeutic levels and its subsequent withdrawal on macrolide resistance using enterococci as an indicator bacterium. fecal samples were collected from steers that received no antibiotics and steers administered tylosin phosphate (11 ppm) in-feed for 197 days and withdrawn 28 days be ... | 2015 | 26074889 |
| antimicrobial resistance profile of enterococcus species isolated from intestinal tracts of hospitalized patients in jimma, ethiopia. | multi-drug-resistant enterococci colonizing the intestinal tract of hospitalized patients are the major source of infection as well as nosocomial spread. despite worldwide increasing rate of multidrug resistant enterococci colonization and infection among hospitalized patients, there is scarcity of data from resource limited setting. the present study aimed at determining the antimicrobial resistance profile of enterococcus species from intestinal tracts of hospitalized patients in jimma, ethiop ... | 2015 | 26036911 |
| diversity of enterococcal species and characterization of high-level aminoglycoside resistant enterococci of samples of wastewater and surface water in tunisia. | one hundred-fourteen samples of wastewater (n=64) and surface-water (n=50) were inoculated in slanetz-bartley agar plates supplemented or not with gentamicin (sb-gen and sb plates, respectively) for enterococci recovery. enterococci were obtained from 75% of tested samples in sb media (72% in wastewater; 78% in surface-water), and 85 enterococcal isolates (one/positive-sample) were obtained. enterococcus faecium was the most prevalent species (63.5%), followed by enterococcus faecalis (20%), ent ... | 2015 | 26026404 |
| prevalence, diversity and characterization of enterococci from three coraciiform birds. | coraciiform birds hoopoe (upupa epops), common kingfisher (alcedo atthis) and european roller (coracius garrulus) were examined for enterococci in their cloacae and uropygial glands. the enterococcal isolates were identified at the species level using several genomic and proteomic methods, screened for antibiotic susceptibility and genotyped by pulsed-field gel electrophoresis (pfge). clonality of isolates from the common kingfisher was also assessed by multi-locus sequence typing (mlst). using ... | 2015 | 25772302 |
| identification of enterococcus faecium and enterococcus faecalis as vanc-type vancomycin-resistant enterococci (vre) from sewage and river water in the provincial city of miyazaki, japan. | as a first step for assessing the risk to human health posed by vancomycin-resistant enterococci (vre) in the aquatic environment, we screened sewage and urban river water samples from miyazaki, japan for vre. because vancomycin-resistant organisms are not as prevalent in sewage and river water as vancomycin-susceptible organisms, the samples were screened by minimum inhibitory concentration test using the vancomycin-supplemented membrane-enterococcus indoxyl-β-d-glucoside (mei) agar. the isolat ... | 2015 | 25438128 |
| diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures? | enterococci represent the most controversial group of dairy bacteria. they are found to be the main constituent of many traditional mediterranean dairy products and contribute to their characteristic taste and flavor. on the other hand, during the last 50 years antibiotic-resistant enterococci have emerged as leading causes of nosocomial infections worldwide. the aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 ente ... | 2015 | 26441888 |
| molecular characterization of microbial contaminants isolated from umbilical cord blood units for transplant. | disposal of umbilical cord blood units due to microbial contamination is a major problem in cord blood banks worldwide as it reduces the number of units available for transplantation. additionally, economic losses are generated as result of resources and infrastructure used to obtain such units. umbilical cord blood units that showed initial microbial contamination were subject to strains isolation, identification, and characterization by sequencing the 16s rrna gene and enterobacterial repetiti ... | 2015 | 26361843 |
| virulence factors genes in enterococci isolated from beavers (castor fiber). | only limited information exists concerning the microbiota in beaver (castor fiber). this study has been focused on the virulence factors genes detection in enterococci from beavers. in general, animals are not affected by enterococcal infections, but they can be a reservoir of, e.g. pathogenic strains. moreover, detection of virulence factors genes in enterococci from beavers was never tested before. free-living beavers (12), male and female (age 4-5 years) were caught in the north-east part of ... | 2015 | 25300356 |
| isolation of alkaline-tolerant bacteria from primary infected root canals. | alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3). | 2015 | 25638530 |
| antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens. | the addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. | 2015 | 26957978 |
| comparison of apical sealing ability of two phases of gutta-percha: a bacterial leakage model. | the aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model. | 2015 | 27507995 |
| effect of different obturation materials on residual antimicrobial activity of 2% chlorhexidine in dentin at different time intervals: an ex vivo study. | the aim of this study was to evaluate the effect of gutta percha/ah26 and resilon/realseal se on residual antimicrobial activity of chlorhexidine (chx) in human root dentin and suggest the best filling material when chx is used as final irrigant. | 2015 | 27252755 |
| characterization of enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production. | in this study, 72 enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr) patterns and biofilm production. of the isolates, 31 (43.1%) were positive for gelatinase, 11 (15.3%) for aggregation substance and cytolysine, 38 (52.8%) for gele and 34 (47.2%) for asa1 genes. all isolates were found ... | 2015 | 27175186 |
| [clinical observations of acute-onset endophthalmitis after clear corneal phacoemulsification]. | to evaluate the incidence of acute-onset endophthalmitis after clear corneal phacoemulsification and to report its clinical features, microbiology, and therapeutic efficacy. | 2015 | 26888274 |
| [the incidence and risk factors of male urinary tract infection following invasive urodynamic investigation]. | to assess the prevalence of male urinary tract infection (uti) after invasive urodynamic studies and to determinate the risk factors by evaluating the clinical characteristics and urodynamic parameters of patients. | 2015 | 26813139 |
| did the iceman know better? screening of the medicinal properties of the birch polypore medicinal mushroom, piptoporus betulinus (higher basidiomycetes). | the birch polypore piptoporus betulinus was among two mushrooms that were found in the iceman's bag. recent studies indicated that p. betulinus was probably used as a religious and medicinal item. in order to examine the medicinal potential of p. betulinus, hot water (hw), partially purified (pp), and alkali extract (ha) were prepared and tested for antioxidant, antimicrobial, cytotoxic, and angiotensin i-converting enzyme (ace) inhibitory activity. all tested samples exhibited moderate cytotoxi ... | 2015 | 26854098 |
| antimicrobial activity of herbal medicines (tulsi extract, neem extract) and chlorhexidine against enterococcus faecalis in endodontics: an in vitro study. | successful endodontic treatment depends on effective disinfection and complete sealing of root canal. various medicaments are advised for disinfecting root canal, such as herbal and non-herbal medicaments. this study was done to assess the antimicrobial activity of herbal medicines (neem extract, tulsi extract) and chlorhexidine against enterococcus faecalis in endodontics. | 2015 | 26942123 |
| enterococcus faecalis biofilm. formation and development in vitro observed by scanning electron microscopy. | biofilm produced by enterococcus faecalis isolated from root canals was detected by growing it on microplates and using 10% crystal violet stain, elution with alcohol and three procedures: no fixation, heat fixation and 10% formaldehyde fixation. the biofilm was evaluated using a versamax microplate reader (usa). twenty sterile root portions were incubated in ts broth with e. faecalis (108) for 48 hours, 4, 7, 14 and 30 days, after which they were processed and observed by scanning electron micr ... | 2015 | 27095620 |
| cholesterol suppresses antimicrobial effect of statins. | isoprenoid biosynthesis is a key metabolic pathway to produce a wide variety of biomolecules such as cholesterol and carotenoids, which target cell membranes. on the other hand, it has been reported that statins known as inhibitors of isoprenoid biosynthesis and cholesterol lowering agents, may have a direct antimicrobial effect on the some bacteria. the exact action of statins in microbial metabolism is not clearly understood. it is possible that statins inhibit synthesis or utilization of some ... | 2015 | 26877857 |
| the effect of addition of an eps degrading enzyme with and without detergent to 2% chlorhexidine on disruption of enterococcus faecalis biofilm: a confocal laser scanning microscopic study. | enterococcus faecalis is one of the most commonly occurring organisms retrieved from root canal treated teeth that show refractory apical periodontitis. though it is well known that the ability of e. faecalis to form a matrix-encased biofilm contributes to its pathogenicity, the role of extracellular dextran and dna in biofilm formation and its effect on the susceptibility of the biofilm to chlorhexidine remains poorly understood. it was hypothesized that the addition of an extracellular polymer ... | 2015 | 26675655 |
| enterococcus faecalis septicemia and vertebral osteomyelitis after transrectal ultrasound guided biopsy of the prostate. | transrectal ultrasound guided prostate biopsy (trus) has rarely been associated with disseminated infection, yet the occurrence appears to be increasing. resistance to fluoroquinolones, the most commonly used prophylaxis, is one of the likely causes, with escherichia coli being the most commonly reported cause of these infections. herein we present what is, to our knowledge, the first case of enterococcus faecalis septicemia and vertebral osteomyelitis after trus. previously reported cases of th ... | 2015 | 26682075 |
| genomic sequence of a clinical vancomycin-resistant reference strain, enterococcus faecalis atcc 51299. | in this paper, we present a draft genome sequence of a quality control reference strain, enterococcus faecalis atcc 51299 (multilocus sequencing type [mlst] st6), which is sensitive to teicoplanin but resistant to vancomycin. it is used in an agar screening test for streptomycin, gentamicin, and vancomycin resistance and the resistance marker vanb. | 2015 | 26679597 |
| [a case of pyoderma gangrenosum of the penis difficult to distinguish from fournier gangrene]. | here, we report a case of pyoderma gangrenosum of the penis which was difficult to distinguish from fournier gangrene. the patient was a 54-year-old male who was aware of redness and swelling of the glanspenis for 1 month prior to a consultation at our department. although he was diagnosed with herpes and treated at a nearby hospital, his symptoms did not improve. subsequently, the patient visited our department following the onset of pain and fever. during his initial consultation, he had a fev ... | 2015 | 26699892 |
| evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of enterococcus faecalis. | background and aims. triple antibiotic paste (tap) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. the aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of tap at 1-, 2-, 3-, and 4-week intervals on mature enterococcus faecalis biofilm. materials and methods. a total of 287 extracted one-rooted human central incisors were infected with e. faecalis atcc 29212 after removing the crown and preparation ... | 2015 | 26697145 |
| the capability of tyramine production and correlation between phenotypic and genetic characteristics of enterococcus faecium and enterococcus faecalis strains. | the aim of this study was to investigate the diversity of tyramine production capability of four enterococcus strains in buffered systems in relation to their genetic characteristics and environmental conditions. cells of the strains enterococcus faecalis ef37 and atcc 29212, and e. faecium fc12 and fc643 were re-suspended in phosphate/citrate buffers with different ph, nacl concentration and incubation temperature. at intervals, cell viability and tyramine production were assessed by plate coun ... | 2015 | 26696981 |
| olive mill waste extracts: polyphenols content, antioxidant, and antimicrobial activities. | natural polyphenols extracts have been usually associated with great bioactive properties. in this work, we investigated in vitro antioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (owwe) and the olive cake extracts (oce). using the folin ciocalteux method, owwe contained higher total phenol content compared to oce (8.90 ± 0.728 g/l versus 0.95 ± 0.017 mg/g). the phenolic compounds identification was carried out with a performance liquid chromatograph coupled ... | 2015 | 26693221 |
| enterococcus faecalis from food, clinical specimens, and oral sites: prevalence of virulence factors in association with biofilm formation. | enterococci have gained significance as the cause of nosocomial infections; they occur as food contaminants and have also been linked to dental diseases. e. faecalis has a great potential to spread virulence as well as antibiotic resistance genes via horizontal gene transfer. the integration of food-borne enterococci into the oral biofilm in-vivo has been observed. therefore, we investigated the virulence determinants and antibiotic resistance of 97 e. faecalis isolates from the oral cavity, foo ... | 2015 | 26793174 |
| global regulation of gene expression by the mafr protein of enterococcus faecalis. | enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. however, as an opportunistic pathogen, it is able to colonize other host niches and cause life-threatening infections. its adaptation to new environments involves global changes in gene expression. the ef3013 gene (here named mafr) of e. faecalis strain v583 encodes a protein (mafr, 482 residues) that has sequence similarity to global response regulators of the mga/atxa family. the enterococcal og1rf genome also e ... | 2015 | 26793169 |
| enterococcus faecalis as multidrug resistance strains in clinical isolates in imam reza hospital in kermanshah, iran. | the current study aimed to investigate the prevalence of vancomycin-resistant enterococcus in e. faecalis and e. faecium and antimicrobial susceptibility patterns, then dominant genes responsible for vancomycin resistance were determined. for this propose, 180 clinical isolates of enterococcus were subjected for identification and antibiotic susceptibility assay. then, the gene responsible vancomycin resistant strains were determined. the results demonstrated the e. faecalis as a dominant entero ... | 2015 | 26738400 |
| microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation. | this study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (cmp). | 2015 | 26521147 |
| in vitro activity of oritavancin and comparator agents against staphylococci, streptococci and enterococci from clinical infections in europe and north america, 2011-2014. | oritavancin is a lipoglycopeptide that has been approved for the treatment of acute bacterial skin and skin-structure infections (absssis) caused by susceptible organisms. oritavancin causes cell death by inhibiting cell wall synthesis as well as depolarising and permeabilising the cellular membrane of gram-positive pathogens. the activities of oritavancin in comparison with vancomycin, daptomycin and linezolid were determined against a collection of over 11000 recent clinical gram-positive isol ... | 2015 | 26541881 |
| comparative evaluation of effect of different irrigation solutions against enterococcus faecalis: a polymerase chain reaction-based study. | enterococcus faecalis is the most isolated or detected species from oral infections including marginal periodontitis, infected root canals, periradicular abscesses and also detected in cases of failed endodontic therapy. to prevent endodontic treatment failure irrigation is mandatory for the effective removal of smear layer, pulp tissue, and microorganisms. cultivation and other traditional identification methods have been demonstrated to have several limitations when it comes to microbiological ... | 2015 | 26538921 |
| actions of probiotics on trinitrobenzenesulfonic acid-induced colitis in rats. | we investigated the actions of probiotics, streptococcus faecalis 129 bio 3b (sf3b), in a trinitrobenzenesulfonic acid- (tnbs-) induced colitis model in rats. after tnbs was administered into the colons of rats for induction of colitis, the rats were divided into two groups: one group was given a control diet and the other group was given a diet containing sf3b for 14 days. there were no apparent differences in body weight, diarrhea period, macroscopic colitis score, and colonic weight/length ra ... | 2015 | 26550572 |
| antibiotic resistance and biofilm formation of enterococcus faecalis in patient and environmental samples. | enterococci are opportunistic pathogens and are a major factor in nosocomial infections. they may contain ebp operon, which upon expression makes them highly prone to biofilm formation on biotic and abiotic surfaces. | 2015 | 26587208 |
| assessment of the potential of cfc (calcium hydroxide flagyl ciprofloxacin) for the rapid disinfection of resilon and gutta-percha. | obturating materials exposed to the dental operating environment has been shown to be contaminated, making rapid chair side disinfection mandatory to ensure the sterility of the root canals. | 2015 | 26557614 |
| biological changes of enterococcus faecalis in the viable but nonculturable state. | enterococcus faecalis may enter a viable but nonculturable (vbnc) state under adverse conditions. e. faecalis, the major bacterial species present in failed root canal treatments, is thought to survive after endodontic treatment by entering a vbnc state. in this study, we characterized the vbnc state of e. faecalis. we designed 3 different protocols to successfully induce the vbnc state. approximately one-third of bacteria entered a vbnc state after 15-30 days, and all remained viable for at lea ... | 2015 | 26600540 |
| synergistic antibacterial effect of the combination of ε-polylysine and nisin against enterococcus faecalis. | this study evaluated the antibacterial effect of the combination of ε-polylysine (ε-pl) and nisin against enterococcus faecalis strains. the combination of ε-pl and nisin showed synergistic antibacterial activity against three enterococcus strains. scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ε-pl and nisin synergistically damaged the cell morphology of e. faecalis strain r612z1 cells. both ε-pl and nisin can dissipate the transmembrane ... | 2015 | 26613915 |
| infective endocarditis involving the pulmonary valve. | pulmonary valve (pv) infective endocarditis (ie) is a rare entity, accounting for 1.5% to 2% of cases of ie. published data are limited to a few case series and reports. we sought to review the mayo clinic experience and describe clinical, echocardiographic, and microbiologic features. we included all patients aged ≥18 years seen from 2000 to 2014 who had a diagnosis of native pv ie and unequivocal echocardiographic involvement of the pv. nine patients with pv ie were identified. isolated pv ie ... | 2015 | 26611123 |
| clinical and microbiological features of bacteremia caused by enterococcus faecalis. | enterococcus faecalis is a frequent etiologic agent of invasive infections in hospitalized patients. the aim of this study was to analyze clinical and microbiological features of bacteremia caused by e. faecalis. | 2015 | 26623628 |
| tricuspid valve endocarditis complicated by mobitz type ii heart block - a case report and literature review. | we present a case of a middle-aged male who manifested with low-grade fever and lower back pain. mri and bone scan of the spine were suggestive of vertebral osteomyelitis. blood cultures were persistently positive for enterococcus faecalis and echocardiogram revealed tricuspid valve endocarditis. there was no history of iv drug use and urine toxicology was negative. ekg showed mobitz type ii av block and a transesophageal echocardiogram revealed no valve ring or septal abscesses. the heart block ... | 2015 | 26653699 |
| antibacterial and toxic effect of hydrogen peroxide combined with different concentrations of chlorhexidine in comparison with sodium hypochlorite. | hydrogen peroxide (h2o2) has been suggested to be used in sequence or in combination with chlorhexidine (chx) to enhance the antibacterial activity against enterococcus faecalis, but there is no research in the literature on the safety and effectiveness of this irrigation protocol. | 2015 | 26636124 |
| in vitro comparison of antimicrobial effectiveness of qmix and other final irrigants in human root canals. | final root canal irrigation stands as an effective strategy for eliminating the dentin infection. this study aimed to investigate and compare the antibacterial efficacy of qmix and other four final irrigation regimens in reducing enterococcus faecalis within human root canals. single-canal human teeth contaminated with e. faecalis for 4 weeks were prepared chemomechanically with sodium hypochlorite (naocl). then, the teeth were randomly assigned into six groups according to the final irrigation ... | 2015 | 26631907 |
| degree of bacterial microleakage at the implant-abutment junction in cone morse tapered implants under loaded and unloaded conditions. | different results have been reported on the internal colonization of cone morse connections under in vitro dynamic loading. the aim of the present in vitro study was to evaluate the bacterial leakage in cone morse implant-abutment connections, both under loaded and unloaded conditions. | 2015 | 26660625 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| an in vitro evaluation of antimicrobial activity of five herbal extracts and comparison of their activity with 2.5% sodium hypochlorite against enterococcus faecalis. | sodium hypochlorite is the most widely used irrigant in endodontic practice, but it has various disadvantages. literature has shown that herbal products such as propolis, azadirachta indica (ai), triphala, curcuma longa, and morinda citrifolia (mc) possess good antimicrobial properties and thus can be used as potential endodontic irrigants. | 2015 | 26672425 |
| anti-microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin: an in vitro study. | sodium hypochlorite (naocl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. | 2015 | 26672416 |
| the influence of urinary tract infections on treatment of fracture of proximal femur in patients with systemiv osteoporosis. | in the structure of intrahospital infections the infections of urinary tract make up from 20 to 40%. this problem represents special medical-social significance for trauma departments as patients with trauma are being in forced situation for along time. 380 patients older than 60 years (average age 73.6 ± 9.2 years) with a fracture of proximal femur against background of the systemic osteoporosis having in-patient treatment in the trauma department of the republic hospital no2 were being examine ... | 2015 | 26887137 |
| antibacterial efficacy of polymer containing nanoparticles in comparison with sodium hypochlorite in infected root canals. | the aim of the present study was to compare the antibacterial properties of plga polymer containing zno, ag and zno/ag nanoparticles with those of 2.5% naocl in root canals contaminated with enterococcus faecalis. | 2015 | 26486202 |
| antimicrobial activity of silver doped fabrics for the production of hospital uniforms. | among several alternatives to control hospital-acquired infections (hais), a strategy could be the use of hospital uniforms imbued with antimicrobial substances. for this purpose we evaluated the antibacterial activity of two different silver doped fabrics employed for the production of hospital uniforms. the study was conducted in two-step. in the first the antimicrobial activity was evaluated in vitro against escherichia coli atcc 25922, pseudomonas aeruginosa atcc 27853, staphylococcus aureus ... | 2015 | 26485013 |
| activity of fosfomycin and comparison of several susceptibility testing methods against contemporary urine isolates. | fosfomycin is recommended as first-line treatment for acute uncomplicated cystitis in women. it has demonstrated in vitro activity against a variety of pathogens; however, a paucity of data are available from the usa. we determined the susceptibility of a collection of urine isolates to fosfomycin and compared multiple methods of susceptibility testing. consecutive non-duplicate enterobacteriaceae, enterococci and pseudomonas aeruginosa isolates were collected from the clinical microbiology labo ... | 2015 | 26498988 |
| selective digestive decontamination and enterococcus faecalis overgrowth and infection: a robust relation is yet unproven. | 2015 | 26495899 | |
| virus satellites drive viral evolution and ecology. | virus satellites are widespread subcellular entities, present both in eukaryotic and in prokaryotic cells. their modus vivendi involves parasitism of the life cycle of their inducing helper viruses, which assures their transmission to a new host. however, the evolutionary and ecological implications of satellites on helper viruses remain unclear. here, using staphylococcal pathogenicity islands (sapis) as a model of virus satellites, we experimentally show that helper viruses rapidly evolve resi ... | 2015 | 26495848 |
| design and activity of a cyclic mini-β-defensin analog: a novel antimicrobial tool. | we have designed a cyclic 17-amino acid β-defensin analog featuring a single disulfide bond. this analog, designated "amc" (ie, antimicrobial cyclic peptide), combines the internal hydrophobic domain of hbd1 and the c-terminal charged region of hbd3. the novel peptide was synthesized and characterized by nuclear magnetic resonance spectroscopy. the antimicrobial activities against gram-positive and gram-negative bacteria as well as against herpes simplex virus type 1 were analyzed. the cytotoxic ... | 2015 | 26508857 |
| structural analysis of streptococcus pyogenes nadh oxidase: conformational dynamics involved in formation of the c(4a)-peroxyflavin intermediate. | in probing the oxygen reactivity of an enterococcus faecalis nadh oxidase (nox; o2 → 2h2o) c42s mutant lacking the cys42-sulfenic acid (cys42-soh) redox center, we provided direct evidence of a c(4a)-peroxyflavin intermediate in the oxidative half-reaction and also described a conformational or chemical change that is rate-limiting for full reoxidation of the homodimer. in this work, the nox from streptococcus pyogenes (spynox) has been expressed and crystallized, and the overoxidized wild-type ... | 2015 | 26506002 |
| functional analysis of genes involved in the biosynthesis of enterocin nkr-5-3b, a novel circular bacteriocin. | a putative biosynthetic gene cluster of the enterocin nkr-5-3b (ent53b), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkb, the ent53b structural gene, using a fosmid library. a region approximately 9 kb in length was obtained, and the enkb1, enkb2, enkb3, and enkb4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of ent53b, were identified. we also determined the identity of proteins mediating self-immun ... | 2015 | 26503847 |
| rapidly progressive gas-containing lumbar spinal epidural abscess. | gas-containing (emphysematous) infections of the abdomen, pelvis, and extremities are well-known disease entities, which can potentially be life-threatening. they require aggressive medical and often surgical treatment. in the neurosurgical field, some cases of gas-containing brain abscess and subdural empyema have been reported. sometimes they progress rapidly and even can cause fatal outcome. however, gas-containing spinal epidural abscess has been rarely reported and clinical course is unknow ... | 2015 | 26512268 |
| influence of a subinhibitory concentration of vancomycin on the in vitro expression of virulence-related genes in the vancomycin-resistant enterococcus faecalis. | exposure to subinhibitory concentrations (sics) of antimicrobials may alter the bacterial transcriptome. | 2015 | 26516976 |
| modeling and application of a rapid fluorescence-based assay for biotoxicity in anaerobic digestion. | the sensitivity of anaerobic digestion metabolism to a wide range of solutes makes it important to be able to monitor toxicants in the feed to anaerobic digesters to optimize their operation. in this study, a rapid fluorescence measurement technique based on resazurin reduction using a microplate reader was developed and applied for the detection of toxicants and/or inhibitors to digesters. a kinetic model was developed to describe the process of resazurin reduced to resorufin, and eventually to ... | 2015 | 26457928 |
| tumor necrosis factor receptor-associated factor 6 plays a role in the inflammatory responses of human periodontal ligament fibroblasts to enterococcus faecalis. | enterococcus faecalis is a frequently isolated microorganism in persistent periapical lesion or secondary infection. however, no evidence has demonstrated that e. faecalis induced inflammation directly in the apical area. this study aimed to explore the mechanism of the inflammatory responses of human periodontal ligament fibroblasts (pdls) to e. faecalis. | 2015 | 26454720 |
| characterization of mobile staphylococcus equorum plasmids isolated from fermented seafood that confer lincomycin resistance. | the complete nucleotide sequences of lincomycin-resistance gene (lnua)-containing plasmids in staphylococcus equorum strains isolated from the high-salt-fermented seafood jeotgal were determined. these plasmids, designated pselnu1-3, are 2638-bp long, have two polymorphic sites, and encode typical elements found in plasmids that replicate via a rolling-circle mechanism including the replication protein gene (rep), a double-stranded origin of replication, a single-stranded origin of replication, ... | 2015 | 26448648 |
| labdane and clerodane diterpenoids from colophospermum mopane. | five labdane (1-5), an isolabdane (6), and five clerodane diterpenoids (7-11), were isolated from seeds, husks, and leaves of colophospermum mopane. compounds 1-3 and 6-9 are new, and their structures were elucidated by means of physical data analysis (1d and 2d nmr, hresims). the absolute configurations of 1, 7, and 10 were determined by single-crystal x-ray diffraction with cu kα radiation. for compounds 2 and 6, the absolute configurations were established by the modified mosher's method and ... | 2015 | 26444248 |
| faster and economical screening for vancomycin-resistant enterococci by sequential use of chromogenic agar and real-time polymerase chain reaction. | screening for vancomycin-resistant enterococci (vre) by culture takes days to generate results, while polymerase chain reaction (pcr) testing directly from clinical specimens lacks specificity. the aims of this study were to develop a real-time pcr to detect and identify enterococcus faecium, enterococcus faecalis, and vana and vanb genes, and to evaluate the impact of this pcr on test-reporting times when performing it directly from suspect vre isolates present on screening chromogenic media. | 2015 | 26442675 |
| clinico-microbiological investigation of catheter associated urinary tract infection by enterococcus faecalis: vana genotype. | prolonged hospitalization and exposure to third generation cephalosporins are reported to facilitate the acquisition and colonization of vancomycin resistant enterococci (vre). though vre is not uncommon in india, urinary tract infection with a vana genotype is a cause of serious concern as vre co-exhibit resistance to aminoglycosides. in india, majority of the vre isolates recovered from hospitalized patients include enterococcus faecium. we report a case of catheter associated urinary tract in ... | 2015 | 26435949 |