Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| porcine reproductive and respiratory syndrome: neb-1 prrsv infection did not potentiate bacterial pathogens. | a 2-phase study was conducted to evaluate the ability of the neb-1 strain of porcine reproductive and respiratory syndrome virus (prrsv) to potentiate common bacterial pathogens of swine. in phase i, 25 of 50 4-5-week-old specific-pathogen-free (spf) pigs were exposed to neb-1 prrsv (day 0). seven days after virus inoculation, 8 groups received 1 of 4 bacterial pathogens: haemophilus parasuis, streptococcus suis, salmonella cholerasuis, and pasteurella multocida. the ability of neb-1 prrsv to pr ... | 1995 | 7578444 |
| description of six new capsular types (29-34) of streptococcus suis. | 1995 | 7578463 | |
| fibrinohemorrhagic pneumonia in pigs naturally infected with streptococcus suis. | 1995 | 7578464 | |
| purification and characterization of a 52-kilodalton immunoglobulin g-binding protein from streptococcus suis capsular type 2. | we previously reported that group d streptococci exhibited immunoglobulin g (igg)-binding activity and that a 52-kda igg-binding protein was present in all streptococcus suis strains examined (b. serhir, r. higgins, b. foiry, and m. jacques, j. gen. microbiol. 139:2953-2958, 1993). the objective of the present study was to purify and characterize this protein. pig igg were immobilized through their fab fragments to ech-sepharose 4b, and the protein was purified by affinity chromatography. electr ... | 1995 | 7601850 |
| septicaemia, meningitis and spondylodiscitis caused by streptococcus suis type 2. | 1995 | 7622264 | |
| alterations in penicillin-binding proteins in strains of streptococcus suis possessing moderate and high levels of resistance to penicillin. | we examined the penicillin-binding proteins (pbps) of certain field strains of streptococcus suis, as well as those from laboratory variants having different degrees of resistance to penicillin. results indicated that (i) s. suis possesses three distinct groups of pbps, arbitrarily named here pbp 1, pbp 2, and pbp 3, with approximate molecular weights of 97, 82, and 45 kda respectively; (ii) pbp profiles of field strains of s. suis having different mics (< or = 0.03) to 16.0 micrograms/ml) were ... | 1995 | 7649432 |
| isolation and identification of streptococcus suis serotypes 3, 4 and 7. | 1995 | 7660562 | |
| streptococcus suis infection. | 1995 | 7662939 | |
| porcine reproductive and respiratory syndrome: clinical disease, pathology and immunosuppression. | porcine reproductive and respiratory syndrome (prrs) was first known as blue-eared pig disease in the united kingdom and the causative agent as 'lelystad virus'. the disease is characterised by very variable clinical signs, including reproductive failure and respiratory disease. the respiratory syndrome is often associated with severe infection with secondary bacterial agents including pasteurella multocida, haemophilus parasuis and streptococcus suis. however, some seropositive herds show no cl ... | 1995 | 7709569 |
| discrimination of virulent and avirulent streptococcus suis capsular type 2 isolates from different geographical origins. | in an effort to relate the protein profile to virulence, proteins from the cellular fractions and from culture supernatants of streptococcus suis capsular type 2 strains from different geographical origins were compared by using western blots (immunoblots). the protein profiles of the cellular fractions were similar for the majority of virulent and avirulent isolates studied, with the exception of three virulent canadian strains for which a 135-kda protein was not detected. examination of the cu ... | 1995 | 7729910 |
| distribution of streptococcus suis capsular types in 1994. | 1995 | 7773923 | |
| characterization of antibiotic resistance in streptococcus suis. | twenty one isolates of streptococcus suis were screened for antibiotic resistance by growth on antibiotic-containing media, by measuring minimum inhibitory concentrations, by hybridization to specific dna and oligonucleotide probes for antibiotic resistance genes, and by pcr. the isolates were from a slaughter house survey of respiratory pathogens in norwegian pigs in 1986. fifteen isolates were resistant to tetracycline, with mics ranging from 4-128 micrograms/ml. genes coding for the tet o and ... | 1994 | 7801524 |
| serotypes and putative virulence markers of streptococcus suis isolates from cats and dogs. | thirteen isolates of streptococcus suis from cats and dogs were characterised. formamide extracts of 10 of the 13 cultures reacted with group d specific antiserum, and serotyping of the cultures with specific antisera against capsular types 1 to 28 revealed the serotypes 9, 20, 1/2, 22, 26 and 4; the remaining cultures were untypable. in addition one of the cultures reacted with monoclonal antibodies against muramidase-released protein and in parallel with monoclonal antibodies against the extra ... | 1994 | 7817019 |
| immunization of mice against streptococcus suis serotype 2 infections using a live avirulent strain. | in this study, the igg response of mice injected with two virulent strains and one avirulent streptococcus suis capsular type 2 strain was compared by western blotting. the serum from mice immunized against the avirulent strain could recognize most proteins of the various strains tested and similar results were obtained with serum from mice injected with virulent strains. the live avirulent strain was injected twice (days 0 and 10) to groups of five mice, and four virulent strains from different ... | 1994 | 7889463 |
| biochemical traits and antimicrobial susceptibility of streptococcus suis isolated from slaughtered pigs. | a total of 522 streptococcus suis isolates were recovered from the tonsils of slaughtered pigs by culturing on media containing different specific antisera. the serogroup reactions were determined by coagglutination. the most frequently isolated serotype was serotype 4 (representing 28.7% of the isolates), followed by serotype 3 (24.9%) and serotype 2 (22.6%). typable isolates were examined by growth in 6.5% nacl, amylase activity, acetoin production, susceptibility to optochin and production of ... | 1994 | 7740861 |
| the prevalence of streptococcus suis type 2 in western australian piggeries. | 1994 | 7726767 | |
| relation between encapsulation and various properties of streptococcus suis. | encapsulation is thought to be a critical virulence factor for streptococcus suis. in this study, encapsulation of a selected s. suis culture could be stimulated by cultivation conditions. after cultivation of the s. suis culture in fluid media supplemented with serum, the culture grew with short chains and a uniform turbidity of the growth medium. autoclave extracts of this culture reacted with capsular type-1-specific antiserum. after cultivation of the s. suis culture in fluid media without s ... | 1994 | 7701857 |
| susceptibility of streptococcus suis to various antimicrobial agents. | a study was performed to determine the susceptibility of 59 s. suis strains, isolated from sick and healthy carrier swine, to a range of antimicrobial agents. the beta-lactamic group proved more active both in kirby-bauer and mci tests. trimethoprim-sulfamethoxazole emerged as an alternative drug in chemoprevention and treatment of s. suis infections. | 1994 | 7597863 |
| genomic relatedness among reference strains of different streptococcus suis serotypes. | hybridization studies using genomic dna and a rdna probe revealed genetic relatedness among reference strains of different streptococcus suis serotypes. although most serotype 22 isolates are biochemically atypical, the reference strain of capsular type 22 is genetically related to other s. suis serotypes, but not to streptococcus pneumoniae. using dna digested with bamhi and bglii for ribotyping, some s. suis reference strains had common patterns, but this analysis mainly revealed variations in ... | 1994 | 7534206 |
| influence of interleukin-1 on neutrophil function and resistance to streptococcus suis in neonatal pigs. | nonspecific immunity is usually lower in neonates than adults. consequently, enhancing the neonate's nonspecific immune capability may be beneficial for the health and growth performance of young animals. we conducted two experiments in which neonatal pigs were injected with recombinant bovine interleukin-1 beta (rboil-1 beta) at 9 to 11 days of age. three consecutive daily injections of rboil-1 beta increased neutrophil and monocyte numbers, which remained elevated until the animals were challe ... | 1994 | 7913118 |
| streptococcus suis meningitis complicated with permanent perceptive deafness: report of a case. | a 61-year-old pig farmer was found to be suffering from meningitis caused by streptococcus suis type ii. he was successfully treated with intravenous penicillin g but was left with permanent deafness. b-cell lymphoma was also diagnosed one year later. s. suis is a zoonotic pathogen which causes meningitis, septicemia and endocarditis in pigs. human infection is rare and often presents as meningitis with the sequela of permanent deafness. it has previously been reported in pig rearing countries s ... | 1994 | 7914782 |
| [sacroiliitis caused by streptococcus suis type ii]. | 1994 | 7918949 | |
| clonal analysis and virulence of australian isolates of streptococcus suis type 2. | multilocus enzyme electrophoresis was used to divide 124 australian isolates of streptococcus suis type 2 into 17 electrophoretic types (ets). isolates in et 1 were the most frequent cause of disease amongst western australian pigs, but isolates of et 8 were more commonly associated with disease in other australian states. multiple isolates from 10 of 19 farms all belonged to the same et, whilst isolates from the other farms belonged to between 2 and 4 different ets. some isolates could be diffe ... | 1994 | 7925669 |
| identification and composition of the streptococcal and enterococcal flora of tonsils, intestines and faeces of pigs. | streptococcus suis was the most frequent streptococcus spp. in pig tonsils, followed by the beta-haemolytic porcine 'equisimilis' ecovar of strep. dysgalactiae. the intestinal streptococcal flora was composed of strep. bovis, strep. hyointestinalis and strep. suis. many of these intestinal strep. suis belonged to a beta-glucuronidase-negative biotype which is infrequent in lesions. nearly half of the strains presumptively identified as strep. alactolyticus produced acid from lactose. this specie ... | 1994 | 7928781 |
| streptococcus suis infection in swine: a retrospective study of 256 cases. part ii. clinical signs, gross and microscopic lesions, and coexisting microorganisms. | a retrospective study of 256 cases of naturally acquired streptococcus suis infections in swine submitted to the indiana animal disease diagnostic laboratory from 1985 to 1989 was undertaken to describe the clinical signs, lesions, and coexisting organisms associated with s. suis serotypes 1-8 and 1/2. infected pigs generally had clinical signs and gross lesions referable to either the respiratory system or to the central nervous system (cns), but not both. neurologic signs were inversely relate ... | 1994 | 7948202 |
| lesions caused by natural infection with streptococcus suis type 9 in weaned pigs. | in this paper we report on the spectrum and frequency of lesions observed in a prospective study of naturally occurring infections with streptococcus suis type 9 (ss-9) in weaned pigs. ss-9 produced arthritis, meningitis, interstitial pneumonia, and endocarditis in 100%, 91%, 73%, and 42%, respectively, of pigs dying from the disease. the lesions were similar to those caused by s. suis type 2 (ss-2), however, ss-9 produced a different frequency of lesions. in ss-2 infections, bronchopneumonia is ... | 1994 | 7948203 |
| comparison of pig, rabbit and mouse igg response to streptococcus suis serotype 2 proteins and active immunization of mice against the infection. | the aim of this study was to compare the igg response of different animal species to streptococcus suis serotype 2 proteins and to evaluate the immunogenic potential of these proteins in the mouse experimental model of infection. the protein profiles of ten different s. suis capsular type 2 isolates were compared by western blotting using antisera produced in mice, rabbits and pigs against the reference strain. strains were grown overnight in todd-hewitt broth, harvested by centrifugation, proce ... | 1994 | 7954125 |
| oligosaccharide-receptor interaction of the gal alpha 1-4gal binding adhesin of streptococcus suis. combining site architecture and characterization of two variant adhesin specificities. | the sugar binding specificities of two groups of streptococcus suis, a pig pathogen that causes meningitis also in man, were determined. both the group represented by a recently characterized strain inhibitable by galactose and n-acetylgalactosamine (type pn) and the group inhibitable by galactose (type po) were found by hemagglutination and solid-phase binding inhibition experiments to recognize the disaccharide gal alpha 1-4gal of the p1 and pk blood group antigens. both types preferred the di ... | 1994 | 7961660 |
| isolation, characterization and protection studies in mice of a streptomycin-dependent mutant of streptococcus suis type 1/2. | an avirulent, streptomycin-dependent (str-d) mutant of streptococcus suis type 1/2 was produced and characterized by its antimicrobial susceptibility, growth kinetics, biochemical reactions and reversion rate. homologous and heterologous vaccine trials in mice resulted in complete protection against challenge with s. suis types 1 and 1/2 and partial protection against challenge with s. suis type 2. | 1994 | 7985376 |
| antimicrobial susceptibility of streptococcus suis isolates. | the antimicrobial activities of penicillin (pen), ampicillin (amp), cephalothin (ct), trimethoprim-sulphamethoxazole (tmp-smx), streptomycin (stm), and gentamicin (gm) against 122 representative strains of streptococcus suis, were compared by the agar dilution procedure. the current us national committee for clinical laboratory standards (nccls) breakpoints for non-enterococcal streptococci were used for pen, amp, ct, and tmp-smx. overall, 50% of strains were not fully susceptible to pen, wherea ... | 1994 | 8044666 |
| partial characterization of streptococcus suis type 2 hemolysin. | streptococcus suis type 2 was evaluated for hemolysin production. supernatants of s. suis type 2 grown in todd-hewitt broth were assayed for hemolytic activity by a photometric assay. twenty-two additional serotypes of s. suis (1,3 to 22, and 1/2) were evaluated for hemolysin production; nine of them (1/2, 1, 4, 5, 14, 15, 17, 19, and 20) were positive. the effects of temperature, atmosphere, centrifugation, sonication, chemicals, bovine serum albumin, fetal calf serum, and enzymes on s. suis ty ... | 1994 | 8051253 |
| distribution of streptococcus suis capsular types in 1993. | 1994 | 8076284 | |
| [streptococcal infections as cause of death in pigs brought in for necropsy]. | research was carried out into the prevalence of streptococcal types isolated from pigs that died of septicaemia, meningo-encephalitis, endocarditis, and pneumonia and which were brought in for investigation from 1 january 1988 to 31 december 1991. cultures were prepared from the liver, spleen, kidneys, and brains of all animals and from the heart valves, joints, bronchi, and lungs of animals with pathological changes. the results are presented in six tables. as a group, streptococci were a major ... | 1994 | 8134911 |
| interaction between streptococcus suis serotype 2 and porcine reproductive and respiratory syndrome virus in specific pathogen-free piglets. | secondary specific pathogen-free (sspf) piglets were inoculated intranasally with streptococcus suis serotype 2 alone, porcine reproductive and respiratory syndrome virus (prrsv) alone, or with prrsv followed by s suis. uninfected piglets were used as controls. pigs inoculated with prrsv (atcc vr-2332) followed by challenge with a virulent strain (87555) of s suis serotype 2 developed clinical signs, suppurative meningitis and large numbers of s suis in their tissues, including the brain and men ... | 1994 | 8135015 |
| evaluation of the antibody response in pigs vaccinated against streptococcus suis capsular type 2 using a double-antibody sandwich enzyme-linked immunosorbent assay. | a double-antibody sandwich enzyme-linked immunosorbent assay (elisa) was standardized for the detection of specific antibodies following vaccination with streptococcus suis capsular type 2 bacterins. no statistically significant increase of antibody titers was detected in vaccinated piglets compared to the nonvaccinated control group, even if a minority of piglets demonstrated an important postvaccinal response. three of four vaccinated sows showed a low antibody response to vaccine and specific ... | 1994 | 8143253 |
| identification of streptococcus suis isolated from swine: proposal for biochemical parameters. | a study was made of the biochemical profiles of 59 strains serotyped as streptococcus suis, isolated from diseased and clinically healthy pigs. the following parameters are proposed for the identification of the species: voges-proskauer negativity, hydrolysis of esculin positivity, trehalose positivity, negativity for growth in 6.5% nacl, and absence of beta-hemolysis on sheep blood agar. s. suis serotype 2 is negative for hippurate, pyrrolidonylarylamidase, and mannose. | 1994 | 8150982 |
| identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of streptococcus suis. | the present report describes the identification, purification, and characterization of a hemolysin produced by streptococcus suis type 2. the hemolysin was purified from the culture supernatant by using different filtration steps, superose-12 column chromatography, and selective (nh4)2so4 precipitation. the purified hemolysin, designated suilysin, had an apparent molecular mass of 54,000 da and exhibited a specific activity of 0.7 x 10(6) hemolytic units per mg. suilysin appeared to belong to a ... | 1994 | 8168935 |
| increase of capsular material thickness following in vivo growth of virulent streptococcus suis serotype 2 strains. | protein profile and capsular material thickness of streptococcus suis serotype 2 strains were compared after in vitro and in vivo growth. three virulent and one avirulent strains were used. these strains were grown in brain heart infusion (bhi) broth, cells were collected by centrifugation, resuspended in a sterile saline solution and injected in diffusion chambers. the devices were then inserted in rat abdomens for 17 h. in vitro grown strains were also inoculated into fresh bhi broth and culti ... | 1994 | 8125242 |
| streptococcus suis infections in birds. | four cases of streptococcus suis infection were diagnosed in psittacine birds and four others in zebrafinches, bullfinches, canaries and a duck. the main clinical and pathological manifestation was septicaemia with multiple sudden deaths in nestlings, young and adults. other cases were solitary, with evidence for secondary involvement of s. suis. four serotypable strains all belonged to serotype 9. | 1994 | 18671137 |
| distribution of the different capsular types of streptococcus suis in nineteen swine nurseries. | 1993 | 17424186 | |
| quebec. distribution of streptococcus suis capsular types in canada in 1992. | 1993 | 17424258 | |
| detection of immunoglobulin-g-binding proteins in streptococcus suis. | this study was undertaken to search for the presence of immunoglobulin g (igg)-binding proteins in streptococcus suis, an important swine pathogen. whole bacterial cells were incubated with human or pig igg conjugated to gold particles and examined by transmission electron microscopy. cells of some s. suis strains were labelled as were cells of the positive control strain, staphylococcus aureus cowan i. binding of pig and human igg to five different bacterial species of group d streptococci, to ... | 1993 | 8126422 |
| streptococci and enterococci associated with tonsils of cattle. | streptococcus suis, streptococcus bovis and the mastitis pathogens streptococcus dysgalactiae and streptococcus uberis were the most frequently occurring streptococci in tonsils of cattle. streptococcus suis dominated in samples from calves between 1 month and 1 year of age, but was much less frequent in calves less than 1 month old. the mastitis pathogen strep. dysgalactiae was found more often in calves than in older animals. enterococci were relatively rare, except in the youngest age group. ... | 1993 | 7763446 |
| distribution of capsular types 1 to 28 and further characteristics of streptococcus suis isolates from various european countries. | most of the 150 streptococcus suis isolates from pigs and ruminants used in this study grew under aerobic conditions and were alpha-hemolytic on sheep blood agar. part of the cultures required an increased co2 concentration. these cultures, representing the co2- dependent ecovar of s. suis, were mainly beta-hemolytic on sheep and horse blood agar and gave a synergistic hemolytic reaction with staphylococcal beta-lysin. similar to s. suis reference cultures, the routine isolates showed typical bi ... | 1993 | 8219510 |
| meningitis in pigs caused by streptococcus suis--a speculative review. | 1993 | 8236779 | |
| population structure of australian isolates of streptococcus suis. | the genetic diversity of 109 isolates of streptococcus suis, which were recovered mainly from australian pigs, was examined by multilocus enzyme electrophoresis. the collection was genetically diverse. sixty-five electrophoretic types (ets) were recognized, with a mean genetic diversity per enzyme locus of 0.512, or 0.431 when the number of isolates in each et was considered. serotype diversity varied, being greatest for isolates of capsular serotype 15 (0.364), and then diminishing in the order ... | 1993 | 8263173 |
| repeats in an extracellular protein of weakly pathogenic strains of streptococcus suis type 2 are absent in pathogenic strains. | streptococcus suis type 2 strains that are pathogenic for pigs produce a 110-kda extracellular protein factor (ef). nonpathogenic and weakly pathogenic strains do not produce ef or produce a protein (ef*) that is immunologically related to ef. to study the pathogenesis of s. suis type 2 in pigs and to develop tools and methods for the control of s. suis type 2 infections, we cloned and characterized the genes encoding ef and various ef* proteins. analysis of the deduced amino acid sequences show ... | 1993 | 8335363 |
| an occurrence of equine transport pneumonia caused by mixed infection with pasteurella caballi, streptococcus suis and streptococcus zooepidemicus. | an acute death occurred in a racehorse with pneumonia after long-distance transportation in december, 1990. pasteurella caballi, streptococcus suis and streptococcus zooepidemicus were isolated from the lung at high rate. specific antigens of these bacteria were also demonstrated immunohistologically in the pneumonic lesion. these findings indicated that the disease is equine transport pneumonia caused by a mixed infection of the three bacterial species. this is the first report on the isolation ... | 1993 | 8357920 |
| use of polyvalent coagglutination reagents for serotyping of streptococcus suis. | polyvalent coagglutination reagents (prs) have been evaluated for the serotyping of streptococcus suis. monovalent antisera produced against 28 s. suis reference strains have been grouped to obtain five different pools. a total of 249 field isolates previously identified and belonging to different serotypes were tested with prs prepared by two different procedures: (i) monovalent coagglutination reagents were individually prepared and mixed in equal proportions, and (ii) antisera were mixed in e ... | 1993 | 8370749 |
| streptococcus suis infection in swine: a retrospective study of 256 cases. part i. epidemiologic factors and antibiotic susceptibility patterns. | a retrospective study of 256 cases of naturally acquired streptococcus suis infections in swine submitted to the indiana animal disease diagnostic laboratory from 1985 to 1989 was performed to determine the epidemiologic factors and antibiotic susceptibility patterns associated with s. suis serotypes 1-8 and 1/2. a standardized computer form was used to record the history, signalment, and clinical signs obtained from the records of selected cases and the microscopic lesions identified after revi ... | 1993 | 8373848 |
| the epidemiological studies of streptococcus suis infections in japan from 1987 to 1991. | the epidemiological studies of s. suis infections in japan were carried out between 1987 to 1991. a total of 380 s. suis strains isolated from pigs, cattle and a horse were serotyped by using antisera against s. suis types 1 to 22. a total of 318 (83.7%) of s. suis isolates were serologically typable. serotype 2 was the most prevalent with 28.2%, followed by type 7 (10.8%), 1/2 (8.4%), 3 (7.4%) and 4 (5.5%). of twenty s. suis strains isolated from cattle, eight were type 9, one was type 10, five ... | 1993 | 8399744 |
| effect of management practices on the streptococcus suis carrier rate in nursery swine. | management practices on swine farms were analyzed to determine factor(s) associated with high prevalence of pigs that were carriers of streptococcus suis. samples were obtained for bacteriologic culture via direct swabbing of palatine tonsils of healthy nursery pigs on 35 farms throughout the united states. overall, 36.7% of the pigs were determined to be carriers. isolates of s suis were serotyped, and antimicrobial susceptibility tests were performed by use of kirby-bauer techniques. streptoco ... | 1993 | 8407494 |
| a sandwich enzyme-linked immunosorbent assay for the detection of streptococcus suis. | a double-antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and the identification of streptococcus suis capsular types 1, 2, 1/2, 3 and 22. the specificity of this test was first evaluated using reference strains of s. suis capsular types 1 to 28 and 1/2 as well as 15 different bacterial species susceptible to be isolated from swine. the elisa developed was very specific for capsular types 1, 3 and 22 but it could not discriminate between capsular types ... | 1993 | 8431800 |
| production of capsular material by streptococcus suis serotype 2 under different growth conditions. | the procedure currently used for the production of streptococcus suis antigen is very long and includes several subcultures. the aim of the present work was to study the in vitro production of capsular material by s. suis serotype 2 after each of these subcultures. the amount of capsular material produced was evaluated by electron microscopy using bacterial cells grown on blood-agar plates and in todd-hewitt broth (thb) or thb supplemented with serum. in addition, the production of antibodies in ... | 1993 | 8431805 |
| the carriage of streptococcus suis type 2 by pigs in papua new guinea. | an indirect fluorescent antibody test was used to detect the presence of streptococcus suis type 2 in nasal and pharyngeal swabs taken from pigs in papua new guinea. the rate of carriage for the two sites in domesticated indigenous village pigs was 0.5 and 2.5% respectively, compared to 39 and 43% for intensively reared pigs. these findings were supported by the results of a serological survey, using an enzyme linked immunosorbent assay, in which 87% of intensively reared pigs but only 8% of vil ... | 1993 | 8432325 |
| characterization of a novel bacterial adhesion specificity of streptococcus suis recognizing blood group p receptor oligosaccharides. | streptococcus suis causes sepsis, meningitis, and other serious infections in piglets, and meningitis in humans. hemagglutination inhibition experiments with mono- and oligosaccharides and glycoproteins indicated that galactose-binding strains of s. suis recognized the gal alpha 1-4gal sequence present in the p1 and pk blood group antigen structures. in thin-layer chromatography overlay assays the bacteria bound to trihexosylceramide (gbo3) but not to globoside (gbo4) or forssman glycolipid (gbo ... | 1993 | 8440715 |
| discrimination between virulent and nonvirulent streptococcus suis type 2 strains by enzyme-linked immunosorbent assay. | discrimination between virulent and nonvirulent strains of streptococcus suis type 2 will allow proper diagnosis of diseased pigs and the identification of carrier pigs. to discriminate between virulent and nonvirulent strains, we developed two double antibody sandwich (das) enzyme-linked immunosorbent assays (elisa) using specific monoclonal antibodies directed against two virulence markers of s. suis type 2. one mab was directed against the 136-kilodalton (kda) cell-wall-associated protein, de ... | 1993 | 8447081 |
| streptococcus suis infection in fallow deer. | 1993 | 8465510 | |
| isolation and distribution of streptococcus suis capsular types from diseased pigs in spain. | a total of 65 isolates of streptococcus suis was recovered from various tissues of diseased pigs. almost 96% of all these isolates could be categorized as one of capsular types 1 to 22 and 1/2. capsular type 2 was the most prevalent and represented 53.8% of all isolates, followed by capsular types 1 (9.2%), 1/2 (7.7%) and 8 (4.6%). brain-meninges, multiples tissues and lungs were the source of the majority of the isolates. a total of 37% of s. suis isolates, mainly from brain-meninges, were foun ... | 1993 | 8122443 |
| conjugation of antibiotic resistance in streptococcus suis. | forty-eight clinical isolates of streptococcus suis were examined for antibiotic sensitivity and the presence of plasmid dna. it was determined that isolates from this study showed a substantial increase in resistance to erythromycin (ery), clindamycin, and tetracycline (tet) compared to a similar study conducted five years earlier. eleven of the 48 isolates contained plasmid dna as revealed by dna isolation and gel electrophoresis. plasmid dna from four strains resistant to the above three anti ... | 1992 | 1313622 |
| inoculation of pigs with streptococcus suis type 2 alone or in combination with pseudorabies virus. | pigs (9 [+/- 1] weeks old) were inoculated with streptococcus suis type 2, pseudorabies virus (prv), or both. for each pig of groups a, b, and c the inoculum of s suis was 10(9) colony-forming units. for each pig of groups a, b, and d the inoculum of prv was 5 x 10(3) tcid50 of either prv strain 4892 (group a, n = 9) or prv isolate b (group b, n = 9). the prv strain 4892 is a highly virulent strain; isolate b causes mild clinical signs of infection in inoculated pigs. group-c pigs (n = 9) were g ... | 1992 | 1317689 |
| molecular analysis of isolates of streptococcus suis capsular type 2 by restriction-endonuclease-digested dna separated on sds-page and by hybridization with an rdna probe. | this study was undertaken to assess the discriminatory value of restriction endonuclease (re) digestion patterns of streptococcus suis chromosomal dna using polyacrylamide gel electrophoresis (sds-page) and dna-rdna hybridization. for the re digestion patterns, dnas were digested separately with the enzymes bamhi and bglii and the resultant fragments were separated by sds-page. an escherichia coli rdna probe derived from pkk3535 was used for the hybridization. twenty-three s. suis capsular type ... | 1992 | 1362584 |
| identification of n-acetylneuraminyl alpha 2-->3 poly-n-acetyllactosamine glycans as the receptors of sialic acid-binding streptococcus suis strains. | streptococcus suis is a common cause of sepsis, meningitis, and other serious infections in young piglets and also causes meningitis in humans. the cell-binding specificity of sialic acid-recognizing strains of streptococcus suis was investigated. treatment of human erythrocytes with sialidase or mild periodate abolished hemagglutination. hemagglutination inhibition experiments with sialyl oligosaccharides indicated that the adhesin preferred the sequence neunac alpha 2-3gal beta 1-4glc(nac). re ... | 1992 | 1400420 |
| [ocular manifestations of selected zoonoses in humans]. | a report is given on the epidemiology and clinical signs of some selected zoonoses that may be of significance for ocular infections in man: brucellosis, leptospirosis, lyme borreliosis, lymphocytic choriomeningitis, newcastle disease, ornithosis (chlamydiosis), rabies, streptococcus suis infection, larva migrans ocularis by toxocara canis or baylisascaris procyonis, toxoplasmosis and tularemia. | 1992 | 1412422 |
| streptococcus suis meningitis. a severe noncompensated occupational disease. | meningitis caused by streptococcus suis type 2, a rare disease first recognized in 1968 (108 cases worldwide in 1989), is contracted by occupational exposure to pigs and often results in very severe disabilities (definitive deafness and ataxia in 50% of cases). we report the case of an employee in a rendering plant whose initial symptom was deafness. a detailed analysis of medical and veterinary literature is provided concerning the epidemiology of the disease, the clinical forms in man, bacteri ... | 1992 | 1432301 |
| persistent diplopia following streptococcus suis type 2 meningitis. | 1992 | 1433136 | |
| testing meningeal strains of streptococcus suis to detect m protein genes. | previous reports have suggested that the surface proteins found in meningeal strains of streptococcus suis might be similar to the m protein of group a streptococci. fifty-five strains of s suis, including human and swine meningeal and pneumonic isolates, were tested for m protein genes by dna probes representing the constant domain of the 3' end of the group a, m protein gene. none of the s suis strains examined was positive, indicating that these organisms either lack m protein genes or harbou ... | 1992 | 1439214 |
| identification and composition of the tonsillar and anal enterococcal and streptococcal flora of dogs and cats. | enterococcus faecalis was the most frequently isolated enterococcal species from anal swabs and tonsils of dogs and cats, although in the anal samples from dogs ent. hirae was found almost as often as ent. faecalis. most ent. faecium strains from dog tonsils differed from those associated with humans and other animals in that they fermented sorbitol. typical ent. avium as well as atypical ent. avium-like strains were seen in dogs, while the related species ent. raffinosus was associated with cat ... | 1992 | 1447058 |
| [acute mortality in pigs caused by streptococcus suis type 1]. | in early 1991 many young pigs died without clinical signs on a farm in the province of utrecht. this article describes the isolation of streptococcus suis type 1 after postmortem examination of the piglets. | 1992 | 1462345 |
| studies on a murine model for evaluation of virulence of streptococcus suis capsular type 2 isolates. | five different parameters, time of incubation of the culture, type of culture medium, inoculum, strain of inbred mice, and age of mice, were tested using the ld50 technique to standardize a murine model for the evaluation of the virulence of streptococcus suis capsular type 2 isolates. a model using 28 day-old mice belonging to cf1 strain appeared to give the best results. the inoculum size was the parameter most influencing the 50% lethal dose obtained with mice. inoculation with 1-ml volume of ... | 1992 | 1490593 |
| production and characterization of two streptococcus suis capsular type 2 mutants. | two avirulent mutants of streptococcus suis capsular type 2 (m2 and m42) were produced from a highly virulent strain. mutant m2, obtained after serial subcultures of the parent strain in the presence of rabbit anti-capsular type 2 serum, no longer possessed the type-specific capsular antigen, as demonstrated by serotyping methods and immunoelectron microscopy. the lancefield group d antigen could not be detected on the cell surface of this mutant using the immunogold labelling technique. sds-pag ... | 1992 | 1536064 |
| an investigation of bacterial causes of arthritis in slaughter hogs. | joints from 153 arthritic and 80 normal slaughter hogs were examined by culture for presence of bacteria. although none of the normal joints yielded bacteria, 37% of the disease joints were positive for bacterial growth. of 67 bacterial isolates obtained, 45% were erysipelothrix rhusiopathiae. occurrence of other bacteria in order of their frequency was streptococcus suis (16%), actinomyces pyogenes (10%), mycoplasma spp. including 3 m. hyorhinis isolates (7%), staphylococci (7%), streptococcus ... | 1992 | 1554766 |
| phenoxymethyl penicillin potassium as an in-feed medication for pigs with streptococcal meningitis. | in two separate studies involving 2500 weaner pigs, strategic infeed medication with phenoxymethyl penicillin potassium significantly reduced the incidence of streptococcal meningitis. | 1992 | 1557880 |
| cloning and nucleotide sequence of the gene encoding the 136-kilodalton surface protein (muramidase-released protein) of streptococcus suis type 2. | we cloned and sequenced the gene encoding the muramidase-released protein (mrp) of a pathogenic streptococcus suis type 2 strain to determine whether its amino acid sequence resembles that of proteins with known functions and to determine its function in virulence. the complete nucleotide sequence composing the gene and the regions flanking it was determined. the deduced amino acid sequence revealed the presence of a signal peptide at the n terminus and a cell envelope anchor at the c terminus, ... | 1992 | 1587602 |
| streptococcus suis infections in horses and cats. | 1992 | 1604787 | |
| antimicrobial resistance patterns and plasmid profiles of streptococcus suis isolates. | streptococcus suis isolates recovered from diseased animals in quebec and western canada and from human cases in europe were tested for their susceptibility to different antimicrobial agents and screened for their plasmid content. most isolates from quebec were clindamycin, erythromycin, and tetracycline resistant; animal isolates from western canada were notably less resistant to clindamycin and erythromycin, whereas human isolates were considerably more susceptible to most antimicrobials teste ... | 1992 | 1616981 |
| porcine streptococcus suis in minnesota. | 1992 | 1616988 | |
| virulence of streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. | to determine whether the virulence of streptococcus suis type 2 is associated with the phenotype of the strain, we infected newborn germfree pigs with 10 strains of s. suis type 2 categorized by three phenotypes. in an earlier study, the phenotypes were distinguished by the presence or absence of the muramidase-released protein (mrp) and an extracellular factor (ef) and were designated mrp+ ef+, mrp+ ef- and mrp- ef-. pigs were first inoculated with bordetella bronchiseptica to predispose them t ... | 1992 | 1730489 |
| distribution of streptococcus suis capsular types in quebec and western canada. | of a total of 561 isolates of streptococcus suis recovered from various tissues of diseased pigs, 464 were from veterinary laboratories in quebec and 97 were from western canada, particularly alberta (84). almost 83% of all these isolates belonged to the 23 known s. suis capsular types. there was no marked difference between the two groups of isolates. capsular type 2 was the most prevalent and represented 32% of all isolates. the other important capsular types were, in decreasing order, 3, 1/2, ... | 1992 | 17423925 |
| quebec. distribution of streptococcus suis capsular types in canada in 1991. | 1992 | 17424026 | |
| characterization of six new capsular types (23 through 28) of streptococcus suis. | six new capsular types of streptococcus suis (types 23 to 28) are described. all reference strains were isolated from diseased pigs and were morphologically and biochemically similar to previously described capsular types 1 to 22. clear and specific reactions were obtained for each of the new capsular types with three different typing techniques; no cross-reactions were detected among them or with other s. suis capsular types. their capsular material presented similar ultrastructural characteris ... | 1991 | 1774266 |
| a case of human endocarditis due to streptococcus suis in north america. | 1991 | 1775866 | |
| experimental infections of mice and pigs with streptococcus suis type 2. | five inbred strains of mice were tested for their susceptibility to streptococcus suis type 2 including the type strain, two isolates from meningitis in pigs and two isolates from tonsils of clinically healthy pigs. c57bl/6, icr and ddy strain mice showed lower susceptibility to all strains of s. suis type 2 than balb/c and ss strain mice. the type strain and the isolates from diseased pigs produced septicaemia and meningitis in balb/c and ss mice inoculated with 10(8) colony forming unit of the ... | 1991 | 1790214 |
| the site of the lesion causing hearing loss in bacterial meningitis: a study of experimental streptococcal meningitis in guinea-pigs. | the pathological basis of hearing loss in bacterial meningitis was investigated using an animal model of streptococcus suis meningitis. forty guinea-pigs were infected after their hearing had been assessed by brain stem auditory evoked potentials. in 17 animals, it was possible to repeat the procedure at the onset of meningitis; this included one animal with subclinical disease. fifteen animals showed evidence of hearing loss, which on subsequent histological examination was found to be associat ... | 1991 | 1800912 |
| an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. iv. bacteriological findings in chronic pneumonic lesions. | a total of 855 pig lungs were collected at slaughter and evaluated macroscopically. bacteriological examinations were carried out on tissue samples from chronic pleuropneumonic lesions (n = 196) and from chronic bronchopneumonic lesions with suppuration (n = 14). samples from normal lung tissue (n = 22) were also included. pasteurella multocida was isolated from 54%, actinobacillus (haemophilus) pleuropneumoniae from 11%, and streptococcus spp. from 14% of the pneumonic lesions, respectively. fr ... | 1991 | 1814189 |
| a longitudinal study of natural infection of piglets with streptococcus suis types 1 and 2. | streptococcus suis types 1 and 2 were detected in nasal swabs taken from five litters of piglets sampled twice weekly from birth. the two types had been detected in all pigs by the time they were 38 and 25 days old respectively with mean ages of first detectable infection being 13.5 and 8.5 days. the prevalence of infection was not affected by housing conditions or the population density of pigs. piglets originating from a sow with vaginal swabs positive for s. suis type 2 were infected earlier ... | 1991 | 1879478 |
| identification of two proteins associated with virulence of streptococcus suis type 2. | the protein profiles of various cell fractions of 180 strains of streptococcus suis type 2, which were isolated from diseased pigs, from healthy pigs when they were slaughtered, and from human patients, were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. the isolates from diseased pigs contained two proteins that were absent in most of the isolates from healthy pigs. one of these proteins was a 136-kda protein that was previously identified as the muram ... | 1991 | 1879937 |
| adherence of streptococcus suis capsular type 2 to porcine lung sections. | the present study was undertaken to evaluate the ability of 33 streptococcus suis capsular type 2 isolates to adhere to frozen sections of porcine lung. twenty isolates originated from diseased pigs and 13 from the nasal cavities of clinically healthy pigs. all isolates from diseased animals adhered to lung sections; isolates from pneumonia adhered, in general, in greater numbers than isolates from meningitis. only four isolates from clinically healthy animals showed a weak adherence to lung sec ... | 1991 | 1889041 |
| identification of epidemic strains of streptococcus suis by genomic fingerprinting. | a natural outbreak of streptococcus suis meningitis in two closed swine herds was studied. dna fingerprinting, serotyping, and biochemical profiles were assessed. multiple serotypes were recovered from these herds. in farm a, 50 s. suis strains were isolated from 330 swabs collected. eighteen strains belonged to serotype 2, and 32 strains belonged to serotypes 3, 5, 6, 8, 9, and 11. in farm b, 16 s. suis strains were recovered from a total of 70 samples. eight strains belonged to serotype 7 and ... | 1991 | 1890177 |
| susceptibility of streptococcus suis to penicillin. | 1991 | 1892937 | |
| fatal streptococcus suis meningitis in man. | a case of streptococcus suis meningitis is observed in a 39-year-old and previously healthy meat factory worker. neurological recovery was incomplete despite adequate and sustained antimicrobial therapy. early deafness was demonstrated by brainstem auditory evoked potentials. death due to aspiration pneumonia, cardiac arrest and subsequent cerebral anoxia occurred late in the course of the illness. | 1991 | 1927255 |
| colonization of the pharyngeal tonsil and respiratory tract of the gnotobiotic pig by a toxigenic strain of pasteurella multocida type d. | seven-day-old gnotobiotic pigs were inoculated intranasally with pasteurella multocida and euthanatized 2, 5, 9, and 14 days after inoculation. tissues from the oropharynx and respiratory tract of pigs were cultured quantitatively and analyzed microscopically. pigs remained afebrile and alert, except one that died of acute fibrinopurulent pneumonia. pasteurella multocida was isolated in greatest numbers from the pharyngeal tonsils, but only in low numbers from turbinate, trachea, lung, spleen, a ... | 1991 | 1949505 |
| detection of streptococcus suis type 2 in tonsils of slaughtered pigs using improved selective and differential media. | new selective and differential media were devised for the isolation and identification of streptococcus suis type 2. the selective medium (nncc) agar) was todd-hewitt broth containing 1.5% bactoagar and 5% defibrinated sheep blood with addition of sodium azide (50 micrograms/ml), nalidixic acid (25 micrograms/ml), colistin (12.5 micrograms/ml) and crystal violet (2 micrograms/ml). the differential medium consisted of heart infusion agar and antiserum specific only for s. suis type 2. in a total ... | 1991 | 1949547 |
| bèta-hemolytic streptococci from pigs: bacteriological diagnosis. | bèta-hemolytic streptococci from lesions in pigs were identified as s. dysgalactiae biotype "equisimilis" and s. dysgalactiae serovar l, s. porcinus, s. agalactiae, e. faecalis and co2 dependent and broad bèta-hemolytic s. suis. data are provided which can be used in the interpretation of commercial identification systems. tests results and physiological characteristics which complete identification procedures using coagglutination tests are proposed and discussed. | 1991 | 1950251 |
| primary prevention of streptococcus suis meningitis. | 1991 | 1682567 | |
| characteristics of different streptococcus suis ecovars and description of a simplified identification method. | streptococcus suis strains associated with different animal hosts or located in different body regions constitute special ecovars of s. suis. a group of co2-dependent strains which showed several unusual traits was found in the genital tract of swine, and mannitol- and sorbitol-fermenting strains were isolated from sheep, cattle and horses. s. suis was found to be biochemically more heterogenous than originally described. a detailed description is given of additional characteristics of the speci ... | 1991 | 2024436 |
| evaluation of methods used for detecting streptococcus suis type 2 in tonsils, and investigation of the carrier state in pigs. | streptococcus suis type 2 was isolated from the tonsils of 143 (76 per cent) of 188 pigs from three herds by culturing on media containing a specific antiserum. culture of frozen sections of tonsillar tissue was more sensitive than culture of swabs or deep scrapes. the specificity of an indirect fluorescent antibody test for identifying s suis type 2 in mixed cultures was conservatively estimated to be 0.89. the level of infection in one herd without a history of meningitis was lower than in two ... | 1991 | 2034898 |
| superoxide dismutases of pathogenic and non-pathogenic streptococcus suis type 2 isolates. | pathogenic and non-pathogenic isolates of streptococcus suis type 2 were screened to determine whether differences in superoxide dismutase (sod) synthesis could explain the observed differences in their pathogenicity and intracellular fate in macrophages. a single band of sod activity of similar rf value was visualised in page gels in all isolates and inhibition studies suggested that the cofactor present was manganese. there was no correlation between specific sod activity and virulence. it is ... | 1991 | 2037239 |
| isolation and characterization of streptococcus suis capsular types 9-22. | the incidence and biochemical patterns of streptococcus suis capsular types 9-22 are presented. of 148 untypeable (with types 1-8 antisera) isolates of s. suis recovered from diseased pigs, 10% were not capsulated. of the remaining 134 isolates, only 53% belonged to capsular types 9-22; capsular types 22 and 9 were the most prevalent, representing 19% and 13%, respectively. capsular type 15 (de moor's group t streptococcus) is reported here for the first time in north america since it was descri ... | 1991 | 2039790 |
| pneumonia in a sow due to streptococcus suis type ii and bordetella bronchiseptica. | 1991 | 2063536 |