Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the wall associated lipoteichoic acid of streptococcus sanguis. | a competitive elisa is described for the measurement of lipoteichoic acid. the assay was used to determine the wall associated lipoteichoic acid of streptococcus sanguis which was found to represent only 2-4% of the phenol extractable content. extracellular lipoteichoic acid was detected even after exhaustive cell washing. this material was not the result of de novo synthesis because membrane de-polarization had no effect on the amount detected. since extracellular lipoteichoic acid interfered w ... | 1993 | 8386915 |
| agglutination of streptococcus mutans serotype c cells but inhibition of porphyromonas gingivalis autoaggregation by human lactoferrin. | the ability of various forms of human lactoferrin (lf) to agglutinate oral streptococcus mutans, strep. sobrinus, strep. rattus, strep. sanguis, porphyromonas gingivalis and actinobacillus actinomycetemcomitans cells was studied spectrophotometrically. fe3+ saturated lf was unable to agglutinate these bacteria, whereas iron-free lf (apo lf) effectively agglutinated strep. mutans cells but not the other bacteria. the efficiency and rate of agglutination of strep. mutans were somewhat lower with a ... | 1993 | 8387771 |
| longitudinal study of relations between human salivary antimicrobial proteins and measures of dental plaque accumulation and composition. | many studies have attempted to relate levels of antimicrobial proteins in saliva to oral health; results have been inconsistent, and one reason might be inconsistency of measures of plaque and saliva within subjects. this study investigated associations between plaque and salivary variables in longitudinal data. whole saliva, and 8-h plaque pooled from buccal first permanent molars, was obtained from 32 dental students on tuesdays from 3:00-6:00 p.m. over 4 weeks. salivary flow rate was determin ... | 1993 | 8392324 |
| the comparative activity of azithromycin, macrolides and amoxycillin against streptococci in experimental infections. | since serious sequelae may follow streptococcal infections, eradication is viewed as necessary for successful therapy. studies were therefore conducted to compare the effectiveness of azithromycin with other macrolide antibiotics and amoxycillin to eliminate these organisms in experimental localized infections. in a streptococcus pneumoniae lung infection induced by transtracheal challenge, the pathogen was not recovered after therapy with azithromycin (ed50 7.9 mg/kg), while clarithromycin was ... | 1993 | 8396094 |
| involvement of alpha 2-adrenoreceptors and g proteins in the modulation of platelet secretion in response to streptococcus sanguis. | in the presence of plasma, human platelets secrete the contents of their dense granules and then aggregate in response to certain strains of streptococcus sanguis. after 2 to 5 min of incubation with streptococci, platelets from fast-responding donors will begin to aggregate. slow responders aggregate after a longer delay. platelets may secrete after a short (fast responder) or long (slow responder) delay because of differences in the basal levels or responses to potentiating catecholamines. to ... | 1993 | 8397001 |
| purification and characterization of chimeric human iga1 and iga2 expressed in cos and chinese hamster ovary cells. | ag-specific chimeric human iga molecules, of the two human subclasses, iga1 and iga2, have been expressed in two mammalian cell systems. analysis of the secreted iga molecules, purified in milligram quantities from stable chinese hamster ovary transfectants by ag affinity chromatography, has allowed a direct comparison of the biologic properties of the two subclasses. hplc gel filtration analysis revealed that in both subclasses, the iga molecules associate predominantly into dimers. the monomer ... | 1993 | 8409433 |
| distribution and isolation frequency of eight streptococcal species in saliva from predentate and dentate children and adults. | the isolation frequency and distribution of eight recently defined streptococcal species have been investigated in the saliva of adults and that of both predentate and dentate children. the effects of frequency of sugar intake and tooth presence on the distribution of streptococcal species were also analyzed. saliva samples were collected from 121 subjects divided into three study groups: (a) 56 predentate children (predentate group), (b) 37 dentate children (dentate group), and (c) 28 adults (a ... | 1993 | 8418104 |
| saliva-binding protein (ssab) from streptococcus sanguis 12 is a lipoprotein. | two lipoprotein consensus sequences (leu-x-x-cys) are found in the presumptive signal peptide region (positions 12 to 15 and 17 to 20) of saliva-binding protein (ssab) from streptococcus sanguis 12. three analogs of ssab containing cys-->gly mutations were constructed by site-directed mutagenesis of psa2, the recombinant plasmid expressing ssab. [3h]palmitate was incorporated into ssab only when the native cys-20 residue was present. these data show that ssab is a lipoprotein and that cys-20 is ... | 1993 | 8419308 |
| home antibiotic therapy for streptococcal endocarditis: a call for a controlled trial. | 1993 | 8420289 | |
| the streptococcus sanguis platelet aggregation-associated protein. identification and characterization of the minimal platelet-interactive domain. | streptococcus sanguis expresses a cell wall-bound protein that induces the activation and aggregation of platelets. this platelet aggregation-associated protein (paap) contains a collagen-like, platelet-interactive domain within a 23-kda protein fragment. to isolate the minimal platelet-interactive domain, p23 paap was digested with collagenase, and the digest chromatographed to isolate fractions with activity inhibitory to s. sanguis-induced platelet aggregation. the active fraction was then di ... | 1993 | 8420939 |
| the dental status and attitudes of patients at risk from infective endocarditis. | infective endocarditis represents one of the few potentially fatal infections that may be caused in the patient by a dentist. efforts to reduce the incidence of this disease usually take the form of appropriate antibiotic cover prior to dental treatment, together with the establishment and maintenance of good oral health. this paper is a report of a survey on the dental health and awareness of 81 'at-risk' patients attending a cardiology outpatient clinic. it was found that whilst the patients w ... | 1993 | 8422317 |
| acute valvular obstruction from streptococcal endocarditis. | 1993 | 8427158 | |
| interfering alpha-streptococci as a protection against recurrent streptococcal tonsillitis in children. | recurrent streptococcal tonsillitis/pharyngitis is a great problem, especially in certain epidemiological situations. patients treated with antibiotics often have a disturbed normal throat flora and may lack, e.g., alpha-streptococci known in vitro to have an interfering activity against group a streptococci. thirty-one patients with recurrent streptococcal tonsillitis were given antibiotics for 10 days. at the end of this treatment they were sprayed in their mouths with four selected alpha-stre ... | 1993 | 8436458 |
| biosynthesis and characterization of phosphatidylglycerophosphoglycerol, a possible intermediate in lipoteichoic acid biosynthesis in streptococcus sanguis. | a membrane enzyme preparation from streptococcus sanguis was shown to convert sn-[14c]glycerol 3-phosphate and cdp-diacylglycerol (or deoxycdp-diacylglycerol) into a series of progressively higher-molecular-weight [14c]oligophosphoglycerophospholipids in vitro. the first oligophosphoglycerophospholipid to accumulate (termed lipid-1) was purified to homogeneity; chemical analysis, gas-liquid chromatography and chemical degradation studies indicated the most likely structure to be phosphatidylglyc ... | 1993 | 8443240 |
| mixed continuous cultures of streptococcus mutans with streptococcus sanguis or with streptococcus oralis as a model to study the ecological effects of the lactoperoxidase system. | mixed continuous cultures of streptococcus species were obtained, using complex carbohydrate (mucin) as a source of nutrients, to study the ecological effects of oxygen and the lactoperoxidase system. s. mutans nctc 10449 was unable to grow as a pure culture on mucin, but attained a significant population size in the presence of s. oralis and s. sanguis strains. the cell densities of the anaerobic mixed cultures decreased when oxygen was supplied, and s. mutans was more suppressed by oxygen than ... | 1993 | 8448770 |
| ph changes in human dental plaque from lactose and milk before and after adaptation. | ph changes in human dental plaque in vivo from lactose and milk have been studied in four separate mouthrinse experiments. (1) ph was measured in plaque samples of 12 subjects before and after 6 weeks of frequent daily mouthrinsing with 10% lactose. the ph decreases were significantly more pronounced after the adaptation period than before (p < 0.001), 4 of the 12 subjects showing about the same low plaque ph from lactose as from glucose. (2) similar to the previous experiment, frequent daily mo ... | 1993 | 8448773 |
| synergistic activity of ceftriaxone combined with netilmicin administered once daily for treatment of experimental streptococcal endocarditis. | we have conducted experiments to determine if one daily injection of netilmicin (net) would be synergistic with the broad-spectrum cephalosporin ceftriaxone (cro) in the treatment of experimentally induced endocarditis. rats with catheter-induced aortic vegetations were infected intravenously with 3 x 10(7) cfu of a beta-lactam-sensitive strain of streptococcus sanguis or a beta-lactam-resistant strain of streptococcus mitis. treatment with the antibiotics alone (cro, 10 mg/kg of body weight eve ... | 1993 | 8452350 |
| infective endocarditis after transesophageal echocardiography. | 1993 | 8462170 | |
| coaggregation: specific adherence among human oral plaque bacteria. | nearly all human oral bacteria exhibit coaggregation, cell-to-cell recognition of genetically distinct cell types. clumps or coaggregates composed of the two kinds of cells are formed immediately upon mixing two partner cell types. members of all 18 genera tested exhibit lactose-reversible coaggregation. many of these interactions appear to be mediated by a lectin on one cell type that interacts with a complementary carbohydrate receptor on the other cell type. a lactose-sensitive adhesin has be ... | 1993 | 8462782 |
| determination by heteronuclear nmr spectroscopy of the complete structure of the cell wall polysaccharide of streptococcus sanguis strain k103. | although complete structures of complex polysaccharides have traditionally been determined by chemical degradative methods, a number of recent developments in instrumentation have greatly facilitated this task. we illustrate the application of several of these methods in a determination of the complete covalent structure of the polysaccharide from streptococcus sanguis k103, which is composed of an octasaccharide repeating subunit linked by phosphodiester bonds. carbohydrate analysis by hpae-pad ... | 1993 | 8470820 |
| infectious crystalline keratopathy. the role of bacterial exopolysaccharide. | infectious crystalline keratopathy is a unique pauci-inflammatory infection of the cornea most commonly due to viridans type streptococci. we investigated the hypothesis that production of exopolysaccharide by streptococci, a property that can be induced by growth conditions, may contribute to the pathogenesis of infectious crystalline keratopathy by suppressing the ocular immune response. | 1993 | 8470987 |
| targeting and delivery of bactericide to adsorbed oral bacteria by use of proteoliposomes. | proteoliposomes having surface-bound succinylated concanavalin a (s-cona) have been prepared from a range of phospholipid mixtures by sonication (suv) and reverse phase evaporation (rev) covering a range of size (weight-average diameter (dw)) from approx. 35 to 310 nm and weight-average number of protein molecules per liposomes (pw) from approx. 50 to 3000. the targeting of the proteoliposomes to adsorbed biofilms of the bacteria streptococcus sanguis and streptococcus mutans has been assessed f ... | 1993 | 8476919 |
| role of superantigens in human disease. | superantigens include bacterial products (mainly of streptococci and staphylococci) that stimulate t cells to proliferate nonspecifically through interaction with class ii major histocompatibility complex products on antigen-presenting cells and then with variable regions on the beta chain of the t cell receptor complex. they include pyrogenic toxins (streptococcal scarlet fever toxins of serotypes a, b, and c, toxic shock syndrome toxin 1, and staphylococcal enterotoxin serotypes a, b, cn, d, e ... | 1993 | 8486972 |
| defence case known as 'heart'. | 1993 | 8494514 | |
| formation of alpha-(1-->6), alpha-(1-->3), and alpha-(1-->2) glycosidic linkages by dextransucrase from streptococcus sanguis in acceptor-dependent reactions. | dextransucrase from streptococcus sanguis 10558 was found to synthesize alpha-(1-->6), alpha-(1-->3), and alpha-(1-->2) linkages during an acceptor-dependent glucosyl transfer reaction. normally, new glucosyl residues are added at c-6 of monosaccharide acceptors. however, sugars blocked at c-6 also can serve as good acceptors. the disaccharide and trisaccharide products formed when methyl 6-bromo-6-deoxy-alpha-d-glucopyranoside was used as acceptor were isolated and characterized. both were foun ... | 1993 | 8495440 |
| xylitol and the bactericidal effect of chlorhexidine and fluoride on streptococcus mutans and streptococcus sanguis. | the present study was made to investigate the effect of xylitol on the bactericidal and bacteriostatic action of chlorohexidine diacetate (chx) and sodium fluoride (f) in atcc strains of streptococcus mutans and s. sanguis. standardized bacterial cell suspensions were used in tests for bactericidal effect and for inhibition of growth and sucrose fermentation. the results showed no interference of xylitol with the antibacterial effect of chx and f combinations. xylitol did not show any additive e ... | 1993 | 8498160 |
| oral streptococcal colonization of infants. | the objective of this study was to determine the prevalence and proportions of different streptococcal species among the streptococcal flora during infancy. a total of 60 oral samples were collected by oral swabbing of the buccal mucosa and alveolar ridges of 18 infants before tooth eruption and from buccal and lingual surfaces of teeth after tooth eruption. a total of 549 isolates on mitis salivarius agar were speciated, principally by recently revised biochemical criteria of kilian et al. stre ... | 1993 | 8510978 |
| dental plaque development on defined streptococcal surfaces. | coaggregations between bacterial species have been widely studied in vitro but not in the mouth. a new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. chips were covered with streptococcus oralis atcc 10557, which coaggregated with many oral species, or streptococcus gordonii s7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. there were no di ... | 1993 | 8510979 |
| an analysis of the subgingival microflora in randomly selected subjects. | the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ... | 1993 | 8510980 |
| cryopreserved homografts in aortic and mitral prosthetic endocarditis: expanding the use of biological tissues in complex cardiac infections. | the case of a 27-year-old male heroin addict suffering from mitral and aortic prosthetic valve endocarditis is presented. double valve re-replacement was performed using cryopreserved aortic homografts. aortic root replacement with coronary re-implantation and intra-atrial valve implantation for mitral valve replacement were the techniques used. despite the fatal outcome of this case, it clearly illustrates the possibilities of expanding the indications for combined complex replacement of heart ... | 1993 | 7719510 |
| species identification of oral viridans streptococci by restriction fragment polymorphism analysis of rrna genes. | oral streptococci formerly classified as streptococcus sanguis have been divided into six genetic groups. methods to identify those species by genotype are needed. this study compared restriction fragment polymorphisms of rrna genes (ribotypes) for seven s. gordonii, three s. sanguis, four s. oralis, three s. mitis, one s. crista, and seven s. parasanguis strains classified in previous dna hybridization studies, as well as one clinical isolate. dna was digested with hindiii, pvuii, hindiii and p ... | 1993 | 7691875 |
| demonstration of antigen-specific immune response against streptococcus sanguis. | the genetic control of streptococcus sanguis antigen response was studied. mice sensitized with inactivated s. sanguis organisms antigen-injected at the base of the tail developed footpad swelling. those with an i-ak,q,r region of h-2 showed a strong footpad response, whereas those with an i-ab,d,s region showed a weak response to s. sanguis cell wall antigen. footpad response was mediated by cd4+,8- t cells by using in vitro monoclonal antibody treatment. similar evidence of genetic control was ... | 1993 | 7694648 |
| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| expression of salivary mucin bacterial aggregating activity: difference with caries. | the low and high molecular weight mucin forms were isolated from saliva of individuals with different caries status and assessed for their bacterial aggregating potential towards s. mutans and s. sanguis. the high molecular weight mucin from both groups exhibited similar protein and carbohydrate content, but the level of covalently bound fatty acids was lower in the caries-resistant group. the mucin from caries-resistant group showed only a weak inhibitory potential, while no inhibitory activity ... | 1992 | 1290456 |
| serum antibody responses to streptococcus mutans antigens in humans systemically infected with oral streptococci. | sera from patients with subacute bacterial endocarditis (sbe) due to streptococcus mutans or other oral streptococci and from normal subjects were assayed by enzyme-linked immunosorbent assay for antibodies to defined s. mutans antigens. antibodies of igg and iga isotypes to ag i/ii and ag iii were greatly elevated in s. mutans-sbe sera, and the iga antibodies in 3 sera included both polymeric and monomeric forms. elevated igm and igg anti-lipoteichoic acid and igg and iga anti-serotype c polysa ... | 1992 | 1299798 |
| profiling of propionibacterium acnes recovered from root canal and blood during and after endodontic treatment. | this report describes the first results of an ongoing study of bacteremia after endodontic treatment of teeth with asymptomatic apical periodontitis. after access cavity preparation, microbiological samples were taken from the root canal under aseptic conditions in 4 single-rooted teeth in 4 patients. in treatment of 2 of the patients, the first 3 reamers (sizes 15-25) were deliberately used to a level 2 mm beyond the apical foramen. in 2 patients the instrumentation ended inside the root canal ... | 1992 | 1302689 |
| [histological study of the structure of artificial plaque]. | five cariogenic bacteria were used to produce a plaque, called artificial plaque, by sequential culture in vitro. the results showed that the artificial plaque is very similar to the natural one in morphology and structure. artificial plaque may be taken as an excellent model in vitro for the studies on cariogenicity of dental plaque. | 1992 | 1304541 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction. | the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ... | 1992 | 1318331 |
| a truncated tn916-like element in a clinical isolate of enterococcus faecium. | a 58.7-kb nonconjugative plasmid (pkq1) previously reported in a clinical isolate of enterococcus faecium was found to contain both a tetm and an erythromycin resistance (erm) determinant. the plasmid contained a region homologous to the a, f, h, and g hincii fragments of tn916. however, the 4.8-kb b fragment of tn916 which contained the tetm determinant was replaced by a 7.3-kb fragment, and the 3.6-kb hincii c fragment of tn916 was missing. an element homologous to tn917 was juxtaposed to the ... | 1992 | 1319593 |
| subgingival temperature (iii). relation to microbial counts. | the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ... | 1992 | 1321846 |
| de novo glucan synthesis by mutants streptococcal glucosyltransferases present in pellicle promotes firm binding of streptococcus gordonii to tooth surfaces. | adherence of 3h-labelled cells of streptococcus gordonii and streptococcus milleri to artificial pellicles prepared from saliva supplemented with glucosyltransferases from mutants streptococci was examined using a new assay for sucrose-dependent cell-to-pellicle attachment. results indicate that s. gordonii, but not s. milleri, could attach tightly to hydroxylapatite surfaces through de novo glucan synthesis by mutants streptococcal glucosyltransferases present in the experimental salivary pelli ... | 1992 | 1327954 |
| characterization of the adherence of porphyromonas gingivalis to oral streptococci. | adherence of porphyromonas gingivalis to strains of streptococcus sanguis and streptococcus mitis deposited on nitrocellulose paper was investigated. a variety of laboratory strains and clinical isolates of p. gingivalis bound to both s. sanguis and s. mitis. binding of p. gingivalis to all but one of the streptococci was not inhibited by salivary molecules. pretreatment of p. gingivalis with periodate and pretreatment of s. sanguis and s. mitis with pronase decreased binding, suggesting that ad ... | 1992 | 1328995 |
| infective endocarditis in hypertrophic obstructive cardiomyopathy. | 1992 | 1331190 | |
| periodontal bone loss in porphyromonas gingivalis-infected specific pathogen-free rats after preinoculation with endogenous streptococcus sanguis. | anaerobic gram-negative bacteria dominate in periodontitis locations, while gram-positive bacteria characterize healthy sites. a well-established gram-positive flora might therefore inhibit the colonization of gram-negative pathogens. the purpose of the present investigation was to examine whether endogenous s. sanguis could prevent, or reduce, periodontal bone loss in rats infected with a virulent p. gingivalis strain. sixty specific pathogen-free wistar rats were divided into 6 groups. doxycyc ... | 1992 | 1334145 |
| sensitization of oral bacteria in biofilms to killing by light from a low-power laser. | biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ... | 1992 | 1334649 |
| antimicrobial effect of a dental varnish, in vitro. | the effects of a polymer based antimicrobial releasing varnish cervitec were investigated against different gram-positive and gram-negative bacterial strains as well as a yeast using the agar diffusion inhibitory test (adt-test in vitro). as positive controls a 1% chlorhexidine gel and 1% aqueous solution of thymol and a placebo polymer varnish without active agents were employed. the test experimental varnish containing 1% chlorhexidine and 1% thymol showed antimicrobial activity against all gr ... | 1992 | 1336221 |
| disinfection of irreversible hydrocolloid impressions: a comparative study. | this paper demonstrates the potential for cross contamination with bacteria and viruses from impression materials and evaluates the efficacy of four disinfection systems on irreversible hydrocolloid impressions contaminated with streptococcus sanguis or poliovirus. an irreversible hydrocolloid impression was made of a contaminated acrylic resin template. the impression was disinfected and residual microorganisms were harvested by sonication, cultured, and counted. the results showed that the imp ... | 1992 | 1339136 |
| isolation and characterization of actinomyces viscosus mutants defective in binding salivary proline-rich proteins. | recent studies have provided evidence for human salivary proline-rich proteins (prps) serving as potential receptors in the acquired pellicle for actinomyces viscosus type 1 fimbriae. we report here the isolation of mutants derived from a. viscosus t14v-j1 which are defective in binding to prps partially purified from parotid gland saliva. mutagenesis with ethyl methanesulfonate preceded enrichment for cells nonreactive with prps by successive adsorptions with prp-treated latex beads. screening ... | 1992 | 1347286 |
| restriction endonuclease-fragment polymorphisms of oral viridans streptococci, compared by conventional and field-inversion gel electrophoresis. | oral streptococci formerly classified as streptococcus sanguis or streptococcus mitis have recently been divided into four species. two additional species have also been proposed for this group. each species is genetically distinct, but they have many traits in common, which makes it difficult for clinical isolates to be identified by phenotypic tests. genotypic comparison may provide an alternative approach. this study used dna fingerprint analysis for comparison of genotypes of 21 reference st ... | 1992 | 1351484 |
| sensitization of oral bacteria to killing by low-power laser radiation. | twenty-seven compounds were screened for their ability to sensitize streptococcus sanguis to killing by light from a 7.3-mw helium/neon (hene) laser. bacteria were mixed with various concentrations of the test compounds, spread over the surfaces of agar plates, and then exposed to light from the hene laser for various time periods. the plates were then incubated and examined for zones of inhibition. those compounds found to be effective photosensitizers were then tested against porphyromonas gin ... | 1992 | 1369193 |
| purification and characterization of an aminopeptidase from streptococcus mitis atcc 903. | an aminopeptidase isolated from the cytoplasmic fraction of a cell extract of streptococcus mitis atcc 903 was purified 330-fold by ion-exchange chromatography, gel filtration, and hydroxyapatite chromatography. the partially purified enzyme had a broad substrate specificity. twelve aminoacyl-beta-naphthylamide substrates were hydrolyzed and also several di-, tri-, tetra-, and pentapeptides and bradykinin. the enzyme hydrolyzed arginine-beta-naphthylamide at the highest rate. optimal conditions ... | 1992 | 1369197 |
| cross-reactive immunodeterminants on streptococcus sanguis and collagen. predicting a structural motif of platelet-interactive domains. | cross-reactive immunodeterminants on a fibril-associated surface antigen of streptococcus sanguis and types i and iii collagen participate in the induction of aggregation of human platelets. to further understand the basis for this apparent molecular mimicry, antitype-specific collagen antibodies, anti-kpgepgpk (an analogue of platelet-interactive domains on collagen) and a panel of kpgepgpk-like synthetic peptides were used as probes. when collagen or s. sanguis cells were pretreated with the a ... | 1992 | 1374395 |
| comparative acid tolerances and inhibitor sensitivities of isolated f-atpases of oral lactic acid bacteria. | ph activity profiles and inhibitor sensitivities were compared for membrane atpases isolated from three oral lactic acid bacteria, lactobacillus casei atcc 4646, streptococcus mutans gs-5, and streptococcus sanguis nctc 10904, with, respectively, high, moderate, and low levels of acid tolerance. membranes containing f1f0 atpases were isolated by means of salt lysis of cells treated with muralytic enzymes. membrane-free f1f0 complexes were then isolated from membranes by detergent extraction with ... | 1992 | 1386211 |
| cloning and sequence analysis of the stsi restriction-modification gene: presence of homology to foki restriction-modification enzymes. | stsi endonuclease (r.stsi), a type iis restriction endonuclease found in streptococcus sanguis 54, recognizes the same sequence as foki but cleaves at different positions. a dna fragment that carried the genes for r.stsi and stsi methylase (m.stsi) was cloned from the chromosomal dna of s.sanguis 54, and its nucleotide sequence was analyzed. the endonuclease gene was 1,806 bp long, corresponding to a protein of 602 amino acid residues (m(r) = 68,388), and the methylase gene was 1,959 bp long, co ... | 1992 | 1387204 |
| glucosyltransferase phase variation in streptococcus gordonii modifies adhesion to saliva-coated hydroxyapatite surfaces in a sucrose-independent manner. | phase variation of streptococcus gordonii between high (spp+) and low (spp-) levels of glucosyltransferase (gtf) activity resulted in the greater adhesion of spp- strains to saliva-coated hydroxyapatite (s-ha) in a washed-cell adhesion test. specific gtf mutants did not show this response. although washed spp+ cells produced 5-fold or more glucan from sucrose than spp- cells did under the conditions of the adhesion test, sucrose elevated the adhesion of both phenotypes to hydroxyapatite (ha) equ ... | 1992 | 1388259 |
| an augmented regenerative technique for severe osseous defects. | techniques for new attachment using the principle of guided tissue regeneration with barrier membranes have become accepted as a method for treating teeth with severe osseous defects and furcation involvement. after an evaluation of existing membranes and techniques, a procedure was developed using an expanded polytetrafluoroethylene soft tissue patch, 1 mm thick (w. l. gore and assoc., flagstaff, az), in conjunction with an alloplast hydroxylapatite bone substitute (ha-500, 40-60 mesh) (orthoma ... | 1992 | 1392503 |
| a case of biliary tract infection caused by haemophilus parainfluenzae. | 1992 | 1396738 | |
| characterization of the streptococcus mutans gs-5 frua gene encoding exo-beta-d-fructosidase. | the complete nucleotide sequence (5,010 bp) of the fructanase gene (frua) and flanking regions of the chromosome of streptococcus mutans gs-5 was determined. the frua gene appears to be the sole transcript arising from a proximal promoter. the presumed precursor of the secreted frua protein consists of 1,423 amino acids, and it has an m(r) of 158,656 and a pi of 4.82. the n terminus of frua has characteristics in common with signal peptides of gram-positive organisms. the c terminus consists of ... | 1992 | 1398976 |
| the platelet interactivity phenotype of streptococcus sanguis influences the course of experimental endocarditis. | a strain of streptococcus sanguis that induced rabbit platelets to aggregate in vitro (agg+ phenotype) was hypothesized to be a more virulent pathogen than an agg- strain in experimental endocarditis in rabbits. a left ventricular catheter was implanted, and then an agg+ or agg- strain was inoculated intravenously. vegetations formed on the aortic semilunar valves but were unaffected by the duration of implantation of the catheter. vegetations enlarged by accumulating platelets and their mass in ... | 1992 | 1398992 |
| longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. | fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ... | 1992 | 1403580 |
| efficacy of preprocedural rinsing with an antiseptic in reducing viable bacteria in dental aerosols. | this double-blind, controlled, cross-over, clinical study evaluated the effect of preprocedural rinsing with an antiseptic mouthrinse on the level of recoverable viable bacteria in an aerosol generated during a typical dental procedure. eighteen subjects participated. following 24 hours of abstention from all oral hygiene procedures, subjects received a 10-minute ultrasonic scaling of a randomly selected one-half of their mouth which served as the unrinsed control. they were then randomly assign ... | 1992 | 1403589 |
| changes in saliva of epileptic patients. | unstimulated whole saliva samples of 27 indoor epileptic patients were studied on their protein composition using biochemical and immunochemical methods. a number of salivary proteins appeared at least partially to be hydrolyzed. in a number of saliva samples the concentration of carbohydrate-containing isoenzymes of amylase was reduced. in addition, the concentration of the 20 kd glycoprotein ep-gp was reduced by 60%. sialic acid, the terminal sugar of the glycoproteins and mucins, was released ... | 1992 | 1403835 |
| purification and properties of sorbitol-6-phosphate dehydrogenase from oral streptococci. | the activity of sorbitol-6-phosphate (s6p) dehydrogenase (s6pdh) and the sorbitol transport system were studied in strains of the oral streptococci streptococcus gordonii, streptococcus mitis, streptococcus sanguis and streptococcus mutans. genetically transformed (to ferment sorbitol) strains and their dna donors were included. s6pdh was purified by anion exchange chromatography and gel filtration. the purity of the enzyme was confirmed by polyacrylamide gel electrophoresis. the purified enzyme ... | 1992 | 1408350 |
| complete structure of the adhesin receptor polysaccharide of streptococcus oralis atcc 55229 (streptococcus sanguis h1). | this report describes the determination of the complete primary structure of the adhesin receptor polysaccharide of streptococcus oralis atcc 55229 (previously characterized as streptococcus sanguis h1), a gram-positive bacteria implicated in dental plaque formation. the polysaccharide was isolated from s. oralis atcc 55229 cells after deproteination, enzymatic hydrolysis, and ion exchange chromatography. it was shown to consist of rhamnose, galactose, glucose, glycerol, and phosphate, in molar ... | 1992 | 1420190 |
| effect of slow-release chlorhexidine mouthguards on the levels of selected salivary bacteria. | this study evaluated the effect of an alternate delivery system for chlorhexidine on salivary levels of mutans streptococci (ms) and other selected oral bacteria. on the basis of salivary ms levels > or = 10(4) cfu/ml, 22 subjects were enrolled. all caries lesions were restored prior to treatment. two pretreatment paraffin-stimulated saliva samples were obtained for culturing at least 1 week apart. complete-arch vacuum-adapted mouthguards were individually fabricated and coated internally with a ... | 1992 | 1423441 |
| bacterial microleakage of cavit, irm, and term. | in this in vitro study, a model system was developed and tested to evaluate the sealing ability of temporary restorative materials used in endodontic access preparations. the materials studied, cavit, irm, and term, were tested on 40 premolars against a known bacterial species, streptococcus sanguis. the leakage of bacterial cells was checked 4 and 8 days after initial immersion in the culture. thermocycling was introduced on the fourth day. after 8 days the cement thicknesses were measured afte ... | 1992 | 1437066 |
| effect of gels containing stannous fluoride on oral bacteria--an in vitro study. | the purposes of this investigation were to evaluate and compare the antimicrobial effect of (1) twelve 0.4 per cent stannous fluoride (snf2) commercial products and (2) different concentrations of snf2 (range = 0.02 to 3.28 per cent). the antibacterial inhibitory effect of various snf2 gels was evaluated as to their effectiveness against oral plaque bacteria including strains of s. mutans, s. sanguis, s. sobrinus, a. viscosus, a. actinomycetemcomitans, and b. intermedius. when twelve different c ... | 1992 | 1444958 |
| expression of m6 protein gene of streptococcus pyogenes in streptococcus gordonii after chromosomal integration and transcriptional fusion. | the m6 protein of streptococcus pyogenes was expressed on the cell surface and secreted in streptococcus gordonii challis (formerly streptococcus sanguis) after chromosomal integration of a promoterless m6 protein gene (emm-6.1). the ermc gene, conferring resistance to erythromycin, was cloned downstream of emm-6.1, within the same clai fragment. the initiation codon of emm-6.1 was 19 bp downstream of a clai site, so that clai cleavage would leave the gene promoterless. the clai fragment contain ... | 1992 | 1448621 |
| amoxycillin-resistant oral streptococci and experimental infective endocarditis in the rabbit. | the ability of three amoxycillin-resistant strains of streptococcus sanguis 254, 24 and 297 (mic 40 mg/l) to cause infective endocarditis (ie) in the rabbit was investigated. these strains all produced infection in the rabbit, as did an antibiotic sensitive control strain nctc 7864. prophylactic amoxycillin (400 mg/kg body weight) administered one hour before bacterial challenge prevented 80% of the animals developing ie irrespective of the challenging strain. it is concluded that amoxycillin-re ... | 1992 | 1452500 |
| interfering alpha-streptococci as a protection against recurrent streptococcal pharyngotonsillitis. | 1992 | 1456123 | |
| alpha-streptococci-inhibiting beta-streptococci group a in treatment of recurrent streptococcal tonsillitis. | 1992 | 1456127 | |
| endocarditis on a left atrial myxoma. | a 55-year-old woman presented with fever and malaise. three blood cultures were positive for streptococcus sanguis. a diagnosis of endocarditis was made and the patient was treated with intravenous penicillin and gentamicin. endocardiography revealed a large left atrial tumour. at operation a myxoma covered by deposits of fibrin was excised. microscopy revealed massive infiltrates of neutrophils and remnants of bacteria, indicating that this myxoma was a nest for infection. | 1992 | 1464350 |
| effects of food preservatives on growth and metabolism of plaque bacteria in vitro and in vivo. | the aims of the present study were to assess the consumption of food preservatives during the last decades, and to study the effect of the preservatives, sorbic and benzoic acid, on growth and glycolysis of oral bacteria in vitro, and on acid formation by dental plaque in vivo. five consumption reports from the central bureau of statistics of norway were used to estimate alterations in consumption of staple food containing the two preservatives. a modified broth dilution method was used to deter ... | 1992 | 1465573 |
| noncariogenicity of erythritol as a substrate. | erythritol is a sugar alcohol produced by aureobasidium sp. from glucose. it is 75-80% as sweet as sucrose and is also nonhygroscopic. the aim of this study was to evaluate this sugar substitute from a cariological point of view. erythritol was neither utilized as a substrate for the lactic acid production nor for plaque formation of mutans streptococci (serotypes a-h) and certain oral microorganisms. it was not utilized for water-insoluble glucan synthesis or cellular adherence by glucosyltrans ... | 1992 | 1468100 |
| streptococcal pharyngitis and epiglottitis in a newborn infant. | we describe a newborn infant with streptococcus sanguis septicaemia and concomitant upper airway obstruction due to epiglottitis and pharyngitis. this rare infection of the supraglottic region was treated with endotracheal intubation and antibiotics. full recovery occurred within 4 days. | 1992 | 1473539 |
| enzyme-linked immunosorbent assay for the detection of antibodies to exocellular proteins of staphylococcus aureus in bone infection. | an enzyme-linked immunosorbent assay (elisa) was developed for measurement of the antibody response to exocellular protein antigens of staphylococcus aureus. the wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of s. aureus. the wells were then reacted sequentially with patient sera, protein a-peroxidase conjugate and chromogenic substrate. serum from patients with s. a ... | 1992 | 1478482 |
| the endodontic microflora revisited. | the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ... | 1992 | 1479923 |
| physico-chemical and structural properties of the surfaces of peptostreptococcus micros and streptococcus mitis as compared to those of mutans streptococci, streptococcus sanguis and streptococcus salivarius. | the surface properties of nine streptococcus mitis and four peptostreptococcus micros strains from the oral cavity were examined and compared with a large group of oral streptococci. zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. in addition, elemental surface concentration ratios were obtained via x-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. the s. mitis and p. mi ... | 1992 | 1487734 |
| [a case report of isolated tricuspid valve endocarditis in a non-addicted adult person without underlying cardiac disease]. | we report a rare case of isolated tricuspid valve endocarditis. a 46-year-old man who was non-addicted, non-alcoholic and had no underlying cardiac disease was admitted to our hospital for persistent pyrexia. appropriate intravenous antibiotics therapy was unable to control the repeated infection and destruction of his tricuspid valve progressed. the patient was referred to our surgical division where on intraoperative inspection, tricuspid valve endocarditis was found to involve the entire ante ... | 1992 | 1491211 |
| macrophage response to bacteria: induction of marked secretory and cellular activities by lipoteichoic acids. | lipoteichoic acids (ltas) from various bacterial species, including staphylococcus aureus, streptococcus pyogenes, streptococcus pneumoniae, enterococcus faecalis, and listeria monocytogenes, were examined for the ability to induce secretory and cellular responses in a pure population of bone marrow-derived mononuclear phagocytes. some of the highly purified ltas, in particular ltas from bacillus subtilis, s. pyogenes, e. faecalis, and enterococcus hirae, were able to affect each of the macropha ... | 1992 | 1500175 |
| mobilization and location of the genetic determinant of chloramphenicol resistance from lactobacillus plantarum catc2r. | the mobilization of a nonconjugative plasmid (pcat) that mediates chloramphenicol resistance in lactobacillus plantarum catc2r was achieved by comobilization with the conjugative plasmid pam beta 1. the conjugation studies confirmed that the 8.5-kb pcat in l. plantarum catc2r contains the gene responsible for chloramphenicol resistance and that the plasmid has several unique restriction sites which make it useful for genetic studies in carnobacterium spp. cloning studies showed that the gene res ... | 1992 | 1513874 |
| the influence of morphological variation on candida albicans adhesion to denture acrylic in vitro. | using denture acrylic pieces coated with either whole human stimulated saliva or oral streptococci, the binding ability of three different candida albicans strains was investigated. the c. albicans strains include a clinical isolate with the commonly observed, smooth, round colonial morphology (strain 613p), a morphological variant spontaneously derived from the clinical isolate strain 613p (strain 613m1bk) and a clinical isolate from an oral lesion that was also a morphological variant upon pri ... | 1992 | 1514934 |
| inhibition of microbial adherence and growth by various glass ionomers in vitro. | this study measured the in vitro inhibition of growth and adherence of five oral bacteria by glass-ionomer materials. disks were prepared from two cavity liners and four restorative class materials, by use of teflon plates with circular wells, five mm wide and two mm deep. the bacterial species tested included: a. viscosus, s. mitis, s. mutans, l. casei, and s. sanguis. growth inhibition studies were performed by the spreading of 0.1 ml of standardized inocula over agar plates produced with sele ... | 1992 | 1521678 |
| enumeration of subgingival species on primary isolation plates using colony lifts. | this study evaluated the feasibility of using a colony lift method and dna probes to enumerate bacterial species cultured on primary isolation plates. fourteen digoxigenin-labeled whole chromosomal dna probes representing 12 subgingival species were validated by hybridization with colony lifts prepared from 249 reference strains of 51 species grown on trypticase soy agar plates supplemented with 5% sheep blood. colonies of reference strains were lifted onto nytran filters from plates and treated ... | 1992 | 1528619 |
| isolation and properties of the capnocytophaga ochracea bacteriocin. | a bacteriocin from a subgingival plaque isolate of capnocytophaga ochracea was purified and characterized. it was isolated from cell extract and had a molecular weight of 100,000. streptococcus sanguis, streptococcus mitis, streptococcus mutans and propionibacterium acnes were susceptible to the bacteriocin. | 1992 | 1528638 |
| induction of a putative laminin-binding protein of streptococcus gordonii in human infective endocarditis. | there is evidence to suggest that the virulence of streptococcus strains in infective endocarditis might be due to the expression of binding sites for the extracellular matrix proteins of damaged valves. in this communication, we draw attention to one laminin-binding protein from a strain of streptococcus gordonii isolated from a patient with human endocarditis. this 145-kda protein was found on the cell wall of the bacterium. the level of expression of this binding protein might be regulated by ... | 1992 | 1530927 |
| noncariogenicity of maltitol in specific pathogen-free rats infected with mutans streptococci. | the effect of maltitol on caries development was examined in an experimental caries system employing specific pathogen-free (spf) sprague-dawley rats. fourteen strains of oral streptococci, including mutans streptococci, did not utilize the maltitol nor produce sufficient acid to demineralize tooth enamel. furthermore, maltitol did not serve as a substrate for glucosyltransferases of either streptococcus mutans mt8148r or streptococcus sobrinus 6715 to synthesize water-insoluble glucan. maltitol ... | 1992 | 1533175 |
| identification of a gene, rgg, which regulates expression of glucosyltransferase and influences the spp phenotype of streptococcus gordonii challis. | streptococcus gordonii challis was previously shown to give rise to phase variants expressing high (spp+) or low (spp-) levels of extracellular glucosyltransferase (gtf) activity. here, shotgun cloning of an s. gordonii spp+ chromosomal digest resulted in a chimeric plasmid (pam5010) able to complement the spp- phenotype. in addition, introduction of pam5010 into an spp+ strain resulted in a 10-fold increase in gtf expression. deletion analysis of pam5010 identified a 1.2-kb dna segment which ex ... | 1992 | 1534326 |
| streptococcal-related antigens stimulate production of il6 and interferon-gamma by t cells from patients with behcet's disease. | greater attention has been recently paid to the role of certain strains of streptococcus as an etiologic agent of behçet's disease, in which t cell abnormalities are considered to be involved. we therefore examined whether t cells from patients with behçet's disease might to be stimulated by streptococcus sanguis-related antigen (rre kth-1 antigens). t cells from 17 patients with behcet's disease, but not those from 13 healthy individuals or from 13 patients with other rheumatic diseases, were s ... | 1992 | 1544169 |
| recurrent meningitis in a child with inner ear dysplasia. | 1992 | 1565564 | |
| retention of streptococci to defined solid surfaces in the presence of saliva secretions. | the initial surface retention of streptococcus sanguis (g9b and atcc 10556) and streptococcus salivarius (atcc 9758 and atcc 13419) was examined using a well defined flow cell system. the microorganisms, known to be recovered from hard vs. soft tissue surfaces, respectively, were suspended in either ringer solution, human parotid saliva (hps), human submandibular and sublingual saliva (hsmsl), or mixed saliva. microbial retention was evaluated on germanium prisms of low (20-25 mnm-1) and medium ... | 1992 | 1574683 |
| multibacterial artificial plaque. a model for studying carious process. | a multibacterial artificial plaque was established in vitro on enamel slabs incubated in mixed culture of streptococcus sanguis, streptococcus mutans (serotype c), streptococcus salivarius, lactobacillus casei and actinomyces viscosus. typical structure same as natural plaque was seen under microscope. the caries-like lesion produced under the plaque was observed in enamel sections. the lesion was similar in appearance to natural ones under optic and scanning electron microscopes. the acids prod ... | 1992 | 1576866 |
| multiple adhesins of streptococci. | 1992 | 1587582 | |
| bioassay for glycopeptides. | 1992 | 1592703 | |
| modulation of human neutrophil adherence by oral bacteria. | polymorphonuclear neutrophils (pmns) comprise over 90 per cent of leukocytes in the oral cavity. although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. adherence of pmns to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. in this study, oral bact ... | 1992 | 1605750 |
| ph regulation by streptococcus mutans. | the intracellular ph (phi) optimum for glycolysis in streptococcus mutans ingbritt was determined to be 7.0 by use of the ionophore gramicidin for manipulation of phi. glycolytic activity decreased to zero as the phi was lowered from 7.0 to 5.0. in contrast, glycolysis had an extracellular ph (pho) optimum of 6.0 with a much broader profile. the relative insensitivity of glycolysis to the lowering of pho was attributed to the ability of s. mutans to maintain a transmembrane ph gradient (delta ph ... | 1992 | 1607433 |
| etiology and antibiotic susceptibility of bloodstream streptococcus sp. | 249 episodes of streptococcus bacteraemia in hospitalized patients were evaluated for both clinical and microbiological features. specification of the isolates demonstrated that infective endocarditis was predominantly associated with streptococcus sanguis and streptococcus bovis whereas streptococcus pyogenes and streptococcus milleri were the most common cause of local and/or systemic infections. in vitro-susceptibility tests towards 9 selected antibiotics proved that beta-lactam antibiotics a ... | 1992 | 1611209 |
| [a clinical study on panipenem/betamipron in chronic respiratory tract infections]. | panipenem/betamipron (cs-976, papm/bp), a new carbapenem antibiotic, was administered a single dose of 500 mg or 750 mg via intravenous drip infusion twice a day for treatment of chronic respiratory infection to study its clinical efficacy, bacteriological efficacy and safety. twenty nine cases were studied for the efficacy evaluation. only the safety evaluation was made in 6 cases which were judged to be unsuitable, because in some of them pneumonia and other diseases were not specified as the ... | 1992 | 1613969 |
| streptococcal and enterococcal bacteremia in patients with cancer. | eighty-two episodes of bacteremia due to streptococci (including the genus enterococcus) in 78 patients hospitalized at institut jules bordet between 1986 and 1988 were reviewed. the incidence ranged from 5.5 to 7.6 per 1,000 admissions (16% of all bacteremias). enterococcus faecalis, streptococcus sanguis, and streptococcus mitis were the most prevalent isolates, followed by streptococcus angionosus, streptococcus salivarius, and large colony-forming beta-hemolytic species (a, b, c, and g). twe ... | 1992 | 1617072 |