Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| characterization and in vitro properties of oral lactobacilli in breastfed infants. | lactobacillus species can contribute positively to general and oral health and are frequently acquired by breastfeeding in infancy. the present study aimed to identify oral lactobacilli in breast and formula-fed 4 month-old infants and to evaluate potential probiotic properties of the dominant lactobacillus species detected. saliva and oral swab samples were collected from 133 infants who were enrolled in a longitudinal study (n=240) examining the effect of a new infant formula on child growth a ... | 2013 | 23945215 |
| photodynamic biofilm inactivation by sapyr--an exclusive singlet oxygen photosensitizer. | prevention and control of biofilm-growing microorganisms are serious problems in public health due to increasing resistances of some pathogens against antimicrobial drugs and the potential of these microorganisms to cause severe infections in patients. therefore, alternative approaches that are capable of killing pathogens are needed to supplement standard treatment modalities. one alternative is the photodynamic inactivation of bacteria (pib). the lethal effect of pib is based on the principle ... | 2013 | 23891675 |
| effects of short-chain fatty acids on actinomyces naeslundii biofilm formation. | actinomyces naeslundii is an early colonizer and has important roles in the development of the oral biofilm. short-chain fatty acids (scfa) are secreted extracellularly as a product of metabolism by gram-negative anaerobes, e.g. porphyromonas gingivalis and fusobacterium nucleatum; and the scfa may affect biofilm development with interaction between a. naeslundii and gram-negative bacteria. our aim was to investigate the effects of scfa on biofilm formation by a. naeslundii and to determine the ... | 2013 | 23731652 |
| in vivo study of the initial bacterial adhesion on different implant materials. | biofilm formation on implant materials plays a major role in the aetiology of periimplantitis. the aim of this study was to examine in vivo the initial bacterial adhesion on six different implant materials. | 2013 | 23694907 |
| effect of metalloporphyrins on red autofluorescence from oral bacteria. | the aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. bacteria were grown anaerobically on tryptic soy agar (tsa) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. the autofluorescence was then assessed at 405 nm excitation. on the tsa without additives, no autofluorescence was observed from any of the species ... | 2013 | 23659237 |
| antimicrobial activity of ethanol extracts of laminaria japonica against oral microorganisms. | laminaria japonica is a brown alga, which is consumed widely in korea, japan, and china. this study investigated the antimicrobial activity of ethanol extracts of l. japonica against oral microbial species to assess the possible application of l. japonica extracts in dental care products. the minimal inhibitory concentration (mic) and minimal bactericidal concentration (mbc) were determined in culture medium using a microdilution method. the mics of ethanol extracts of l. japonica with oral stre ... | 2013 | 23583539 |
| antibacterial activity of probiotic candidates for oral health. | the aim of this study was to determine the probiotic potential of autochthonous oral lactobacilli. for this, 66 strains were screened for antibacterial activity against two cariogenic strains (streptococcus mutans and actinomyces viscosus) and two periodontopathogenic strains (fusobacterium nucleatum and porphyromonas gingivalis). the inhibitory activity was investigated with the agar overlay technique. positive results led us to explore some mechanisms of action. the ability to produce h(2)o(2) ... | 2013 | 23211763 |
| porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of prommp9 and its activation. | recent epidemiological studies have revealed a significant association between periodontitis and oral squamous cell carcinoma (oscc). furthermore, matrix metalloproteinase 9 (mmp9) is implicated in the invasion and metastasis of tumour cells. we examined the involvement of porphyromonas gingivalis, a periodontal pathogen, in oscc invasion through induced expression of prommp and its activation. prommp9 was continuously secreted from carcinoma sas cells, while p. gingivalis infection increased pr ... | 2013 | 23991831 |
| microbiological evaluation of bacterial and mycotic seal in implant systems with different implant-abutment interfaces and closing torque values. | the aim of this study was to evaluate the possible leakage of 3 species of bacteria (streptococcus sanguinis, fusobacterium nucleatum, and actinomyces odontolyticus) and of candida albicans and candida glabrata in osseointegrated implants with different implant-abutment interface (iai) geometry. | 2013 | 23782848 |
| association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females. | previous findings of an association between 25-hydroxyvitamin d [25(oh)d] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin d. to the best of the authors' knowledge, no study has investigated the association between 25(oh)d and pathogenic oral bacteria, a putative cause of periodontal disease. | 2013 | 24261910 |
| a new type of na(+)-driven atp synthase membrane rotor with a two-carboxylate ion-coupling motif. | the anaerobic bacterium fusobacterium nucleatum uses glutamate decarboxylation to generate a transmembrane gradient of na⁺. here, we demonstrate that this ion-motive force is directly coupled to atp synthesis, via an f₁f₀-atp synthase with a novel na⁺ recognition motif, shared by other human pathogens. molecular modeling and free-energy simulations of the rotary element of the enzyme, the c-ring, indicate na⁺ specificity in physiological settings. consistently, activity measurements showed na⁺ s ... | 2013 | 23824040 |
| toll-like receptor 2-dependent activity of native major outer membrane protein proteosomes of chlamydia trachomatis. | chlamydia trachomatis is the most common sexually transmitted bacterial pathogen and the etiologic agent of blinding trachoma. intracellular signaling pathways leading to host cell inflammation and innate immunity to chlamydia include those mediated by toll-like receptors (tlrs) and nucleotide binding oligomerization domain 1 (nod1) protein. in epithelial cells, tlr-dependent signaling contributes to local immune responses via induction of inflammatory mediators. there is evidence that tlr3, tlr ... | 2013 | 23132491 |
| oral epithelial cell responses to multispecies microbial biofilms. | this report describes the use of a novel model of multispecies biofilms to stimulate profiles of cytokines/chemokines from oral epithelial cells that contribute to local inflammation in the periodontium. streptococcus gordonii (sg)/s. oralis (so)/s. sanguinis (ss) and sg/fusobacterium nucleatum (fn)/porphyromonas gingivalis (pg) biofilms elicited significantly elevated levels of il-1α and showed synergistic stimulatory activity compared with an additive effect of the 3 individual bacteria. only ... | 2013 | 23300185 |
| the predominant bacteria isolated from radicular cysts. | to detect predominant bacteria associated with radicular cysts and discuss in light of the literature. | 2013 | 24011184 |
| analysis of bacterial flora associated with peri-implantitis using obligate anaerobic culture technique and 16s rdna gene sequence. | to analyze and characterize the predominant bacterial flora associated with peri-implantitis by using culture techniques under obligate anaerobic conditions and 16s rdna gene sequences. | 2013 | 24278920 |
| anaerobic co-culture of mesenchymal stem cells and anaerobic pathogens - a new in vitro model system. | human mesenchymal stem cells (hmscs) are multipotent by nature and are originally isolated from bone marrow. in light of a future application of hmscs in the oral cavity, a body compartment with varying oxygen partial pressures and an omnipresence of different bacterial species i.e. periodontitis pathogens, we performed this study to gain information about the behavior of hmsc in an anaerobic system and the response in interaction with oral bacterial pathogens. | 2013 | 24223777 |
| antimicrobial activity of isothiocyanates (itcs) extracted from horseradish (armoracia rusticana) root against oral microorganisms. | the antimicrobial activity of isothiocyanates (itcs) extracted from horseradish root was investigated against oral microorganisms: 6 strains of facultative anaerobic bacteria, streptococcus mutans, streptococcus sobrinus, lactobacillus casei, staphylococcus aureus, enterococcus faecalis and aggregatibacter actinomycetemcomitans; one strain of yeast, candida albicans, and 3 strains of anaerobic bacteria, fusobacterium nucleatum, prevotella nigrescens, and clostridium perfringens. the itcs extract ... | 2013 | 24077540 |
| phototoxic effect of blue light on the planktonic and biofilm state of anaerobic periodontal pathogens. | the purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures. | 2013 | 23678390 |
| the connection between ruptured cerebral aneurysms and odontogenic bacteria. | patients with ruptured saccular intracranial aneurysms have excess long-term mortality due to cerebrovascular and cardiovascular diseases compared with general population. chronic inflammation is detected in ruptured intracranial aneurysms, abdominal aortic aneurysms and coronary artery plaques. bacterial infections have been suggested to have a role in the aetiology of atherosclerosis. bacteria have been detected both in abdominal and coronary arteries but their presence in intracranial aneurys ... | 2013 | 23761916 |
| comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: a clinical and microbiologic study. | gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. | 2013 | 23633772 |
| in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota. | the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro. | 2013 | 23368885 |
| development of quantitative real-time pcr primers for detecting 42 oral bacterial species. | in this study, we introduced species-specific quantitative real-time pcr (qpcr) primers designed based on a dna-dependent rna polymerase beta-subunit gene (rpob) for detecting 42 oral bacterial species. the specificity of the qpcr primers was confirmed by conventional pcr with the genomic dnas of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. the standard curves revealed the lower detection limits of 42 bacterial species-specific qpcr primers ra ... | 2013 | 23689247 |
| oral community interactions of filifactor alocis in vitro. | filifactor alocis is a gram positive anaerobe that is emerging as an important periodontal pathogen. in the oral cavity f. alocis colonizes polymicrobial biofilm communities; however, little is known regarding the nature of the interactions between f. alocis and other oral biofilm bacteria. here we investigate the community interactions of two strains of f. alocis with streptococcus gordonii, fusobacterium nucleatum, porphyromonas gingivalis and aggregatibacter actinomycetemcomitans, organisms w ... | 2013 | 24098460 |
| porphyromonas gingivalis, treponema denticola and toll-like receptor 2 are associated with hypertensive disorders in placental tissue: a case-control study. | to explore the associations between the presence of periodontal pathogens and the expression of toll-like receptors (tlr-2 and tlr-4) in the placental tissue of patients with hypertensive disorders compared to the placentas of healthy normotensive patients. | 2013 | 23711357 |
| peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents. | the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function. | 2013 | 23510146 |
| salivary detection of periodontopathic bacteria in fanconi's anemia patients. | fanconi's anemia (fa) is characterized by bone marrow failure and can lead to infections such as periodontal disease. the aim of this study was to compare the prevalence of four periodontopathic bacteria including aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum and treponema denticola in saliva samples from children with and without fa. paraffin-stimulated saliva samples were collected from 71 children and adolescents, aged 6-18 years old. the samples wer ... | 2013 | 24055631 |
| the antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. | the antibacterial activity of various saturated fatty acids (sfa) and unsaturated fatty acids (usfa) against different oral pathogens which are implicated in the cause of dental caries, stomatitis, gingivitis, and periodontitis was examined. the saturated fatty acids pa, sta and ara, and the unsaturated omega-7 fatty acids pla and omega-9 fatty acids oa showed either none to low antimicrobial activity against all of the 12 oral pathogenic strains used in this study. in contrast, the omega-3 pufa ... | 2013 | 24640241 |
| bacterial antagonism against periodontopathogens. | the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria. | 2013 | 22897652 |
| specificity of antimicrobial peptide ll-37 to neutralize periodontopathogenic lipopolysaccharide activity in human oral fibroblasts. | the antimicrobial peptide ll-37 is known to have a potent lipopolysaccharide (lps)-neutralizing activity in various cell types. because of observed heterogeneity within periodontopathogenic lps, the authors hypothesized that ll-37 had specificity to neutralize such lps activity. the present study, therefore, aims to investigate the lps-neutralizing activity of ll-37 to various periodontopathogenic lps in interleukin-8 (il-8) production after challenging them in human oral fibroblasts. | 2013 | 22443521 |
| modulation of quaternary structure and enhancement of ligand binding by the k-turn of tandem glycine riboswitches. | most known glycine riboswitches have two homologous aptamer domains arranged in tandem and separated by a short linker. the two aptamers associate through reciprocal "quaternary" interactions that have been proposed to result in cooperative glycine binding. recently, the interaptamer linker was found to form helix p0 with a previously unrecognized segment 5' to the first aptamer domain. p0 was shown to increase glycine affinity, abolish cooperativity, and conform to the k-turn motif consensus. w ... | 2013 | 23249744 |
| [evaluation on antimicrobial efficacy of fufang bingpeng irrigant: an in vitro study]. | to evaluate the antimicrobial efficacy of fufang bingpeng irrigant and bingpeng irrigant on four endodontic pathogen bacteria. | 2013 | 23991570 |
| antibacterial effect of different root canal sealers on three bacterial species. | bacteria persisting in the root canal system may thwart endodontic therapy. it is therefore interesting to know whether clinically available root canal sealers have an antimicrobial effect. the objective of the present in vitro study was to investigate the antibacterial effect of various sealers on the endodontologically detectable species enterococcus faecalis (e. faecalis), fusobacterium nucleatum (f. nucleatum) and porphyromonas gingivalis (p. gingivalis). | 2013 | 23523285 |
| the effects of stress hormones on growth of selected periodontitis related bacteria. | the focus of this study was to examine in vitro the effects of stress hormones (catecholamines: epinephrine, norepinephrine, dopamine and hydrocortisone: cortisol) on the growth of four anaerobic species of periodontitis-related bacteria (fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and tannerella forsythia) and one facultative anaerobic species (eikenella corrodens). bacterial growth was determined by two different methods: fluorescence in situ hybridization (fish), ... | 2013 | 24036419 |
| subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis. | there is a bidirectional relationship between periodontal disease and type-2 diabetes mellitus (dm). inflammatory mediators may negatively affect glycemic control, and increased glucose levels and resultant glycation end-products may alter the host response against bacterial infection. however, no agreement has been reached regarding the effect of dm on periodontal subgingival microbiota. therefore, the purpose of the present study was to compare the subgingival biodiversity in deep periodontal ... | 2013 | 22762355 |
| adhesion of aggregatibacter actinomycetemcomitans and fusobacterium nucleatum on bioactive tio(2) surfaces. | bioactive tio(2) coatings have been found to enhance fibroblast adhesion and gingival attachment on the titanium surfaces, but no information is available whether the coatings also promote the adhesion of periodontal pathogens. | 2013 | 22220677 |
| comparative microbial analysis of paired amniotic fluid and cord blood from pregnancies complicated by preterm birth and early-onset neonatal sepsis. | 16s rrna-based genomic analyses have revolutionized our understanding of infectious diseases. many cases which were recognized as "idiopathic" are now known to have an infectious etiology. here, we present a proof-of-concept study to examine the microbial link between intra-amniotic infection (iai) and early-onset neonatal sepsis (eons). | 2013 | 23437088 |
| in vitro activity of biapenem plus rpx7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria. | biapenem is a carbapenem being developed in combination with rpx7009, a new inhibitor of serine β-lactamases. biapenem was tested alone and in combination with fixed concentrations of rpx7009 by agar dilution against 377 recent isolates of anaerobes. a separate panel of 27 isolates of bacteroides spp. with decreased susceptibility or resistance to imipenem was also tested. comparator drugs included meropenem, piperacillin-tazobactam, ampicillin-sulbactam, cefoxitin, ceftazidime, metronidazole, c ... | 2013 | 23529731 |
| identification of a 43-kda outer membrane protein of fusobacterium necrophorum that exhibits similarity with pore-forming proteins of other fusobacterium species. | a pair of primers was designed in an attempt to amplify outer membrane protein (omp) gene of fusobacterium necrophorum based on nucleotide sequence of the omp of fusobacterium nucleatum. further analysis was performed to characterize its molecular properties and phylogeny in the genus fusobacterium. we identified a predicated 43kda outer membrane protein (43k omp) in f. necrophorum, which showed the same properties as other pore-forming proteins of gram-negative anaerobic bacteria according to a ... | 2013 | 23433684 |
| oxygen deprivation affects the antimicrobial action of ll-37 as determined by microplate real-time kinetic measurements under anaerobic conditions. | some agents, including escherichia coli and group a streptococcus pyogenes cause infections in oxygen depleted sites. ll-37 is a human host defence peptide shown previously to play an important role in controlling infections caused by these bacteria. however, the effect of oxygen levels on the antimicrobial activity of ll-37 remains obscure. in order to test the effect of oxygen (or lack thereof) on ll-37's activity against e. coli and s. pyogenes, a method for adapting commonly used microtiter ... | 2013 | 23664904 |
| folding and stability of outer membrane protein a (ompa) from escherichia coli in an amphipathic polymer, amphipol a8-35. | amphipols are a class of amphipathic polymers designed to maintain membrane proteins in aqueous solutions in the absence of detergents. denatured β-barrel membrane proteins, like outer membrane proteins ompa from escherichia coli and foma from fusobacterium nucleatum, can be folded by dilution of the denaturant urea in the presence of amphipol a8-35. here, the folding kinetics and stability of ompa in a8-35 have been investigated. folding is well described by two parallel first-order processes, ... | 2013 | 23370791 |
| the lipid bilayer-inserted membrane protein bama of escherichia coli facilitates insertion and folding of outer membrane protein a from its complex with skp. | folding of β-barrel membrane proteins, either from a urea-unfolded form or from chaperone-bound aqueous forms, has been characterized for pure lipid bilayers. the impact of preinserted integral proteins from biomembranes has not been examined in biophysical comparisons, but this knowledge is important for the characterization of protein assembly machinery in membranes to distinguish specific effects from unspecific effects. here, folding was studied for a β-barrel membrane protein, outer membran ... | 2013 | 23641708 |
| lemierre's syndrome: a rare case of pulmonic valve vegetation. | lemierre's syndrome is an uncommon complication of pharyngitis commonly associated with an anaerobic gram negative bacterium, fusobacterium necrophorum. the syndrome usually affects young healthy adults with the mean age of 20 and is characterized by recent pharyngitis followed by ipsilateral internal jugular vein thrombosis and septic thromboembolism. the treatment is at least 6 weeks of antibiotics; the role of anticoagulation is unclear. the following presentation is a case of lemierre's synd ... | 2013 | 23573433 |
| advancement in the routine identification of anaerobic bacteria by maldi-tof mass spectrometry. | we evaluated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) biotyper as a tool for the identification of anaerobic bacteria compared with 500 base-pair (bp) 16s ribosomal ribonucleic acid (rrna) gene sequencing analysis, which is considered to be the "gold standard" method. a total of 484 anaerobic bacteria were retrieved from the clinical specimens of 318 pediatric patients. molecular identification resulted in 18 genera and 51 species. the most prev ... | 2013 | 23584672 |
| an investigation of the presence of specific anaerobic species in necrotic primary teeth. | different microbial identification methods have shown that the microbial community profiles in endodontic infections are diverse and assorted. the aim of this study was to evaluate the frequency of selected endodontic pathogens in the pulp chambers (pcs) and root canals (rcs) of infected primary teeth using pcr methods. paired pc and rc samples were collected from 15 subjects and analyzed by pcr for the presence of filifactor alocis, fusobacterium nucleatum, parvimonas micra, porphyromonas endod ... | 2013 | 23538426 |
| fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment. | increasing evidence links the gut microbiota with colorectal cancer. metagenomic analyses indicate that symbiotic fusobacterium spp. are associated with human colorectal carcinoma, but whether this is an indirect or causal link remains unclear. we find that fusobacterium spp. are enriched in human colonic adenomas relative to surrounding tissues and in stool samples from colorectal adenoma and carcinoma patients compared to healthy subjects. additionally, in the apc(min/+) mouse model of intesti ... | 2013 | 23954159 |
| fusobacterium nucleatum promotes colorectal carcinogenesis by modulating e-cadherin/β-catenin signaling via its fada adhesin. | fusobacterium nucleatum (fn) has been associated with colorectal cancer (crc), but causality and underlying mechanisms remain to be established. we demonstrate that fn adheres to, invades, and induces oncogenic and inflammatory responses to stimulate growth of crc cells through its unique fada adhesin. fada binds to e-cadherin, activates β-catenin signaling, and differentially regulates the inflammatory and oncogenic responses. the fada-binding site on e-cadherin is mapped to an 11-amino-acid re ... | 2013 | 23954158 |
| genotype is an important determinant factor of host susceptibility to periodontitis in the collaborative cross and inbred mouse populations. | periodontal infection (periodontitis) is a chronic inflammatory disease, which results in the breakdown of the supporting tissues of the teeth. previous epidemiological studies have suggested that resistance to chronic periodontitis is controlled to some extent by genetic factors of the host. the aim of this study was to determine the phenotypic response of inbred and collaborative cross (cc) mouse populations to periodontal bacterial challenge, using an experimental periodontitis model. in this ... | 2013 | 23937452 |
| oral fusobacterium nucleatum subsp. polymorphum binds to human salivary α-amylase. | fusobacterium nucleatum acts as an intermediate between early and late colonizers in the oral cavity. in this study, we showed that f. nucleatum subsp. polymorphum can bind to a salivary component with a molecular weight of approximately 110 kda and identified the protein and another major factor of 55 kda, as salivary α-amylase by time-of-flight mass spectrometry and immuno-reactions. salivary α-amylase is present in both monomeric and dimeric forms and we found that formation of the dimer depe ... | 2013 | 23906425 |
| a periodontal pathogen treponema denticola hijacks the fusobacterium nucleatum-driven host response. | periodontitis is a polymicrobial disease that arises from the dysbiosis of the plaque biofilm. to study polymicrobial interactions with gingival epithelial cells, the oral commensal fusobacterium nucleatum and the periodontal pathogen treponema denticola were chosen due to their opposing effects on the expression of human beta-defensins (hbds) and interleukin (il)-8 in gingival epithelial cells. immortalized gingival epithelial hok-16b cells were infected with either f. nucleatum or t. denticola ... | 2013 | 23897119 |
| myocardial infection due to fusobacterium nucleatum. | fusobacterium nucleatum is an anaerobic gram-negative bacillus, which inhabits the oropharynx, gastrointestinal tract, and female genital tract. infections classically affect the head and neck. we report a patient with a myocardial mass due to f. nucleatum, initially thought to be a neoplasm, and discuss anaerobic cardiac infections. | 2013 | 24094834 |
| cetylpyridinium chloride suppresses gene expression associated with halitosis. | halitosis is a common complaint affecting the majority of the population. mouthrinses containing cetylpyridinium chloride (cpc) have been used as oral hygiene aids to suppress oral malodor. although the clinical efficacy of these mouthrinses has been well-documented, the mechanism whereby cpc reduces malodor is less-well-understood. we hypothesized that cpc suppresses expression of the genes (mgl and cdl) and enzymes responsible for methyl mercaptan (ch3sh) and hydrogen sulfide (h2s) production ... | 2013 | 24112735 |
| differential effect of autoinducer 2 of fusobacterium nucleatum on oral streptococci. | autoinducer 2 (ai-2) is a quorum sensing molecule and plays an important role in dental biofilm formation, mediating interspecies communication and virulence expression of oral bacteria. fusobacterium nucleatum connects early colonizing commensals and late colonizing periodontopathogens. f. nucleatum ai-2 and quorum sensing inhibitors (qsis) can manipulate dental biofilm formation. in this study, we evaluated the effect of f. nucleatum ai-2 and qsis on biofilm formation of streptococcus gordonii ... | 2013 | 24112724 |
| draft genome sequence of fusobacterium nucleatum subsp. animalis chdc f324, isolated from a human subgingival plaque in the republic of korea. | five subspecies of fusobacterium nucleatum have been classified: animalis, nucleatum, polymorphum, vincentii, and fusiforme. f. nucleatum subsp. animalis chdc f324 (kcom 1325) was isolated from a human subgingival plaque in the republic of korea. here, we report the draft genome sequence of the strain. | 2013 | 24336380 |
| draft genome sequence of fusobacterium nucleatum subsp. nucleatum chdc f316, isolated from a human peri-implantitis lesion in the republic of korea. | fusobacterium nucleatum is a gram-negative anaerobe and is one of the causative agents of periodontal diseases, including peri-implantitis. fusobacterium nucleatum subsp. nucleatum chdc f316 (kcom 1322) was isolated from a human peri-implantitis lesion. here, we report the draft genome sequence of this strain. | 2013 | 24336379 |
| draft genome sequence of fusobacterium nucleatum subsp. vincentii chdc f8, isolated from a human subgingival plaque in the republic of korea. | fusobacterium nucleatum is a gram-negative, nonmotile, obligately anaerobic rod bacterium which might play an important role in the initiation and progression of periodontal diseases. f. nucleatum subsp. vincentii chdc f8 (kcom 1231) was isolated from a human gingivitis lesion. here, we report the draft genome sequence of the strain. | 2013 | 24336378 |
| suppression of t-cell chemokines by porphyromonas gingivalis. | porphyromonas gingivalis is a major pathogen in periodontal disease and is associated with immune dysbiosis. in this study, we found that p. gingivalis did not induce the expression of the t-cell chemokine ip-10 (cxcl10) from neutrophils, peripheral blood mononuclear cells (pbmcs), or gingival epithelial cells. furthermore, p. gingivalis suppressed gamma interferon (ifn-γ)-stimulated release of ip-10, itac (cxcl11), and mig (cxcl9) from epithelial cells and inhibited ip-10 secretion in a mixed i ... | 2013 | 23589576 |
| host susceptibility to periodontitis: mapping murine genomic regions. | host susceptibility to periodontal infection is controlled by genetic factors. as a step toward identifying and cloning these factors, we generated an a/j x balb/cj f2 mouse resource population. a genome-wide search for quantitative trait loci (qtl) associated with periodontitis was performed. we aimed to quantify the phenotypic response of the progenies to periodontitis by microct analysis, to perform a genome-wide search for qtl associated with periodontitis, and, finally, to suggest candidate ... | 2013 | 23539559 |
| interactions between lactobacillus crispatus and bacterial vaginosis (bv)-associated bacterial species in initial attachment and biofilm formation. | certain anaerobic bacterial species tend to predominate the vaginal flora during bacterial vaginosis (bv), with gardnerella vaginalis being the most common. however, the exact role of g. vaginalis in bv has not yet been determined. the main goal of this study was to test the hypothesis that g. vaginalis is an early colonizer, paving the way for intermediate (e.g., fusobacterium nucleatum) and late colonizers (e.g., prevotella bivia). theoretically, in order to function as an early colonizer, spe ... | 2013 | 23739678 |
| necrotizing pneumonia in children: report of 41 cases between 2006 and 2011 in a french tertiary care center. | forty-one children hospitalized for necrotizing pneumonia were retrospectively analyzed. necrotizing pneumonia represented 0.8% of community-acquired pneumonia and 6% of hospitalized community-acquired pneumonia. the chest radiograph revealed necrosis on admission in onethird of cases. twenty-one cases (51%) were documented, including 13 staphylococcus aureus, all panton-valentine leukocidin positive, 7 streptococcus pneumoniae and 1 fusobacterium nucleatum. | 2013 | 23722529 |
| the protective effect of recombinant foma-expressing lactobacillus acidophilus against periodontal infection. | a number of studies have shown that the outer membrane protein foma found in fusobacterium nucleatum demonstrates great potential as an immune target for combating periodontitis. lactobacillus acidophilus is a useful antigen delivery vehicle for mucosal immunisation, and previous studies by our group have shown that l. acidophilus acts as a protective factor in periodontal health. in this study, making use of the immunogenicity of foma and the probiotic properties of l. acidophilus, we construct ... | 2013 | 23644821 |
| meningitis due to fusobacterium nucleatum after a kart crash. | 2013 | 24471173 | |
| halitosis vaccines targeting foma, a biofilm-bridging protein of fusobacteria nucleatum. | halitosis (bad breath) is estimated to influence more than half of the world's population with varying degree of intensity. more than 85% of halitosis originates from oral bacterial infections. foul-smelling breath mainly results from bacterial production of volatile sulfur compounds (vscs) such as hydrogen sulfide and methyl mercaptan. to date, major treatments for elimination of oral malodor include periodontal therapy combined with antibiotics or antimicrobial agents, and mechanical approache ... | 2013 | 23865430 |
| autoinducer 2 of fusobacterium nucleatum as a target molecule to inhibit biofilm formation of periodontopathogens. | periodontitis is initiated by bacteria in subgingival biofilms, which are composed mostly of gram-negative anaerobes. autoinducer 2 (ai-2) is a universal quorum sensing (qs) molecule that mediates intergeneric signalling in multispecies bacterial communities and may induce biofilm formation. as fusobacterium nucleatum is the major coaggregation bridge organism that links early colonising commensals and late pathogenic colonisers in dental biofilms via the accretion of periodontopathogens, we hyp ... | 2013 | 22633049 |
| method to quantify live and dead cells in multi-species oral biofilm by real-time pcr with propidium monoazide. | real-time pcr (qpcr) is a widely used technique in analysing environmental and clinical microbiological samples. however, its main limitation was its inability to discriminate between live and dead cells.recently, propidium monoazide (pma) together with qpcr has been used to overcome this problem, with good results for different bacterial species in different types of samples.our objective was to implement this technique for analysing mortality in multi-species oral biofilms formed in vitro with ... | 2013 | 23289803 |
| presence of oral bacterial species in primary endodontic infections of primary teeth. | knowledge of the microbial composition of deciduous endodontic infections is limited. this study aimed to evaluate the presence of the 10 oral bacterial species in samples from primary tooth root canals by using microarray technology and to determine the association of these organisms with clinical conditions. | 2013 | 24683780 |
| a case of adolescent pelvic inflammatory disease caused by a rare bacterium: fusobacterium nucleatum. | pelvic inflammatory disease (pid) is a polymicrobial infection that typically occurs in sexually active females. pid is rare in premenarchal and/or noncoital young women; however, there are case reports of pid occurring in virginal females. we present the first reported case of pid associated with f. nucleatum. in this unusual case of pid in an adolescent, the responsible organism may have originated from the patient's oral cavity. | 2013 | 23619431 |
| macrolides inhibit fusobacterium nucleatum-induced muc5ac production in human airway epithelial cells. | fusobacterium nucleatum is one of the most common anaerobic bacteria in periodontitis and is responsible for several extraoral infections, including respiratory tract diseases. in this study, we examined whether f. nucleatum induces mucin secretion in airway epithelial cells. we also examined the effects of macrolides on f. nucleatum-induced mucus production compared with the effects of other antibiotics that exert anti-anaerobic activities. the production of muc5ac, the major core protein of mu ... | 2013 | 23380724 |
| characterization of extracellular polymeric matrix, and treatment of fusobacterium nucleatum and porphyromonas gingivalis biofilms with dnase i and proteinase k. | biofilms are organized communities of microorganisms embedded in a self-produced extracellular polymeric matrix (epm), often with great phylogenetic variety. bacteria in the subgingival biofilm are key factors that cause periodontal diseases; among these are the gram-negative bacteria fusobacterium nucleatum and porphyromonas gingivalis. the objectives of this study were to characterize the major components of the epm and to test the effect of deoxyribonuclease i (dnase i) and proteinase k. | 2013 | 23372876 |
| characteristics of porphyromonas gingivalis lipopolysaccharide in co-culture with fusobacterium nucleatum. | porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. they can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. the lipopolysaccharide (lps) of p. gingivalis shows different virulence by growth condition. the purpose of this study was to investigate the characteristics of p. gingivalis lps when co-cultured with fusobacterium nucleatum. ... | 2013 | 23347381 |
| clinical and microbiological results following nonsurgical periodontal therapy with or without local administration of piperacillin/tazobactam. | we assessed if adjunct administration of piperacillin/tazobactam added clinical and microbiological treatment benefits. | 2013 | 23334268 |
| exploiting preq(1) riboswitches to regulate ribosomal frameshifting. | knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. here we report on a novel in vitro assay to study preq(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preq(1)). the assay is based on the ability of the preq(1) aptamer to fold, upon ligand binding, into a pseudoknotted structure that is capable of stimulating -1 ribosomal frameshifting (-1 fs). aptamers from three diff ... | 2013 | 23327288 |
| dna binding to hydroxyapatite: a potential mechanism for preservation of microbial dna. | molecular methods are increasingly being deployed for analysis of the microbial flora in the root canal. such methods are based on the assumption that recovered dna is associated with the active endodontic infection, yet paleomicrobiology research is based on the recovery of ancient dna from centuries-old tooth and bone samples, which points to considerable longevity of the dna molecule in these tissues. the main component of dentin and bone is the mineral hydroxyapatite. this study assessed dna ... | 2013 | 23321233 |
| prevalence of β-lactamase-producing bacteria in human periodontitis. | beta-lactam antibiotics prescribed in periodontal therapy are vulnerable to degradation by bacterial β-lactamases. this study evaluated the occurrence of β-lactamase-positive subgingival bacteria in chronic periodontitis subjects of usa origin, and assessed their in vitro resistance to metronidazole at a breakpoint concentration of 4 μg/ml. | 2013 | 23173872 |
| diagnosis and treatment of fusobacterium nucleatum discitis and vertebral osteomyelitis: case report and review of the literature. | case report. | 2013 | 23132536 |
| mycoplasma pneumoniae preceding lemierre's syndrome due to fusobacterium nucleatum complicated by acute epstein-barr virus (ebv) infectious mononucleosis in an immunocompetent host. | we report an unusual case of lemierre's syndrome due to a rare species of fusobacterium, that is, fusobacterium nucleatum preceded by mycoplasma pneumoniae pharyngitis and followed later by epstein-barr virus infectious mononucleosis. | 2013 | 22464641 |
| effects of cranberry components on human aggressive periodontitis gingival fibroblasts. | aggressive periodontitis (agp) causes rapid periodontal breakdown involving agp gingival fibroblast production of cytokines [i.e. interleukin (il)-6, a bone metabolism regulator], and matrix metalloproteinase (mmp)-3. lipopolysaccharide upregulates fibroblast il-6 and mmp-3, via transcription factors (i.e. nf-κb). cranberry (vaccinium macrocarpon) inhibits lipopolysaccharide-stimulated macrophage and normal gingival fibroblast activities, but little is known of its effects on agp fibroblasts. ob ... | 2013 | 23106206 |
| protective potential of non-dialyzable material fraction of cranberry juice on the virulence of p. gingivalis and f. nucleatum mixed infection. | periodontitis is a polymicrobial infectious disease. a novel potential chemical treatment modality may lie in bacterial anti-adhesive materials, such as cranberry juice fractions. the aim of this study is to explore the effect of high molecular weight cranberry constituent (non-dialyzable material [ndm]) on the virulence of a mixed infection with porphyromonas gingivalis and fusobacterium nucleatum in mice. | 2013 | 23030239 |
| maternal oral origin of fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16s-23s rrna gene intergenic transcribed spacer region. | fusobacterium nucleatum, a common gram-negative anaerobe prevalent in the oral cavity, possesses the ability to colonize the amniotic cavity and the fetus. however, f. nucleatum may also be part of the vaginal microbiota from where it could reach the amniotic tissues. due to the heterogeneity of f. nucleatum, consisting of five subspecies, analysis at the subspecies/strain level is desirable to determine its precise origin. the aims of this study were: (i) to evaluate the use of the 16s-23s rrna ... | 2013 | 23002071 |
| isolation and identification of porphyromonas spp. and other putative pathogens from cats with periodontal disease. | the purpose of this study was to evaluate the subgingival microbiota and determine the most prevalent periodontal pathogens implicated in feline periodontal disease and to correlate these findings with the clinical periodontal status. subgingival microbiological samples were taken under sedation from 50 cats with clinical signs of periodontal disease. pooled paper point samples from 4 selected subgingival sites were cultured on blood agar and on dentaid-1 medium. suspected pathogens were identif ... | 2013 | 24660305 |
| fusobacterium nucleatum subsp. fusiforme gharbia and shah 1992 is a later synonym of fusobacterium nucleatum subsp. vincentii dzink et al. 1990. | on the basis of the dna-dna hybridization patterns and phenotypic characteristics, fusobacterium nucleatum was classified into five subspecies. previous studies have suggested that f. nucleatum subsp. vincentii is genetically similar to f. nucleatum subsp. fusiforme. the aim of this study was to investigate the possibility of classifying these two subspecies into a single subspecies by phylogenetic analysis using a single sequence (24,715 bp) concatenated 22 housekeeping genes of eight f. nuclea ... | 2013 | 23263257 |
| virulence mechanism of bacteria in mixed infection: attenuation of cytokine levels and evasion of polymorphonuclear leukocyte phagocytosis. | the objective of the present study is to evaluate the effect of bacterial viability on the virulence of mixed infection. | 2013 | 23259412 |
| antimicrobial activities of stearidonic and gamma-linolenic acids from the green seaweed enteromorpha linza against several oral pathogenic bacteria. | we found that the edible green seaweed enteromorpha linza displayed potent antimicrobial activity against prevotella intermedia and porphyromonas gingivalis. to elucidate the active component of e. linza, isolation procedures were performed. | 2013 | 28510876 |
| micronutrient modulation of nf-κb in oral keratinocytes exposed to periodontal bacteria. | chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. we have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including nf-κb and cytokine signalling, when exposed to the periodontal bacteria porphyromonas gingivalis and fusobacterium nucleatum in vitro. the purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro ... | 2013 | 22890546 |
| association between oral malodour and periodontal disease-related parameters in the general population. | to determine the association between halitosis detection and periodontal status in systemically healthy non-smokers and to assess whether halitosis was related to quantities of fusobacterium nucleatum and porphyromonas gingivalis on the tongue dorsum. | 2013 | 22339235 |
| ascorbate and α-tocopherol differentially modulate reactive oxygen species generation by neutrophils in response to fcγr and tlr agonists. | periodontitis, a ubiquitous chronic inflammatory disease, is associated with reduced antioxidant defences and neutrophil hyperactivity in terms of reactive oxygen species (ros) generation. its phenotype is thus characterized by oxidative stress. we have determined the effect of antioxidant micronutrients ascorbate and α-tocopherol on neutrophil ros generation. peripheral neutrophils from periodontally-healthy individuals (n = 20) were challenged with phorbol myristate acetate, igg-opsonised stap ... | 2013 | 22914919 |
| fretibacterium fastidiosum gen. nov., sp. nov., isolated from the human oral cavity. | sgp1(t), a strain belonging to a lineage of the phylum synergistetes with no previously cultivated representatives was subjected to a comprehensive range of phenotypic and genotypic tests. for good growth the strain was dependent on co-culture with, or extracts from, selected other oral bacteria. cells of strain sgp1(t) were asaccharolytic and major amounts of acetic acid and moderate amounts of propionic acid were produced as end products of metabolism in peptone-yeast extract-glucose broth sup ... | 2013 | 22493171 |
| human neutrophil defensins and their effect on epithelial cells. | the aims of the present study include: 1) to localize human neutrophil defensin-1 (hnp-1) through hnp-3 in gingiva and in neutrophil extract-treated epithelial cell monolayers; 2) to determine the effects of hnp-1 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of hnp-1 on the bacterial adherence to epithelial cells. | 2013 | 22443519 |
| handlight retroillumination in hotv visual acuity testing. | 2013 | 4062629 | |
| salt intake, intracellular cations, and hypertension. | 2013 | 3760913 | |
| [risks of postcoital contraception using a d-norgestrel-ethinyl estradiol combination]. | 2013 | 3726780 | |
| initiation of blood coagulation at artificial surfaces. | 2012 | 3439730 | |
| [surgical treatment of primary chronic pancreatitis]. | surgical methods of treatment of chronic alcoholic pancreatitis must be chosen with special reference to the state of the duct and parenchyma of the pancreas. treatment of alcoholic patients should be performed with the participation of psychiatrists. | 2012 | 3424549 |
| proceedings from a workshop symposium of the 14th international congress of chemotherapy. kyoto, japan, june 23-28, 1985. | 2012 | 3510504 | |
| cancer, stress and personality: a correlational investigation of life-events, repression-sensitization and locus of control. | this study investigated the hypothesis that stress-inducing life experiences and the personality factors of external locus of control and tendency to repress emotion are relevant in the aetiology of cancer. fifty cancer patients scored higher than 50 matched controls on a life-events scale (especially negative events), but were found to be lower on repression of emotion. there was no difference between groups on locus of control. | 2012 | 3401426 |
| Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by modulating the filament's length and width. | FadA, a novel adhesin of periodontal pathogen Fusobacterium nucleatum is composed of two forms, pre-FadA and mature FadA (mFadA), constituting the functional FadA complex (FadAc). By electron microscopy, we observed that mFadA formed uniformly long and thin filaments, while FadAc formed heterogeneous filaments of varying lengths and widths, as well as "knots". Mutants in signal peptide or in the non-alpha-helical loop retaining heterogeneous structures had binding activity while those forming ag ... | 2012 | 22108653 |
| fusobacterium nucleatum biofilm induces epithelial migration in an organotypic model of dento-gingival junction. | background: effects of fusobacterium nucleatum biofilm on epithelial cell proliferation, apoptotic cell death and basement membrane constituent collagen iv production were examined in an organotypic dento-epithelial (od-e) model. methods: the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing tooth pieces on the top. a 3-day-old biofilm of either a laboratory strain (atcc 25586) or a clinical strain (ahn 9508) of f. nucleatum was placed on ... | 2012 | 22248219 |
| synergistic effect on biofilm formation between fusobacterium nucleatum and capnocytophaga ochracea. | the formation of dental plaque biofilm by specific gram-negative rods and spirochetes plays an important role in the development of periodontal disease. the aim of this study was to characterize biofilm formation by fusobacterium nucleatum and capnocytophaga ochracea. coaggregation between f. nucleatum and capnocytophaga species was determined by visual assay. biofilm formation was assessed by crystal violet staining. enhancement of biofilm formation by f. nucleatum via soluble factor of c. ochr ... | 2012 | 22252100 |
| [expression of cytokines in dentogingival sulcus in patients after dental implantation and during development of peri-implantitis]. | determination of cytokine content in various areas of dentition in patients with peri-implantitis associated with parodontopathogenic bacteria species of i and ii order. | 2012 | 23297645 |
| antibacterial activity of essential oils against periodontal pathogens: a qualitative systematic review. | periodontal diseases are among the most common infectious diseases that lead to the destruction of periodontal tissues. anaerobic gram-negative bacteria (aggregatibacter actinomecetemcomitans, porphyromonas gingivalis, fusobacterium nucleatum...) isolated from periodontal lesions, have been shown to be related to the onset and progression of periodontal disease. given the incidence of periodontitis, increased resistance of oral bacteria to antibiotics and adverse effects of some antibacterial ag ... | 2012 | 23513511 |
| variations in the oral anaerobic microbial flora in relation to pregnancy. | pregnancy gingivitis is a major oral infection. periodontium acts as a reservoir of inflammatory mediators and sub gingival biofilms of bacteria. | 2012 | 23285437 |