Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| bartonella strains from ground squirrels are identical to bartonella washoensis isolated from a human patient. | the most likely animal source of a human case of cardiac disease in washoe county, nev., was identified by comparison of dna sequences of three genes (citrate synthase glta, 60-kda heat shock protein gene groel, and 16s rrna gene) of bartonella washoensis cultured from the human patient in question and of bartonella isolates obtained from the following nevada rodents: peromyscus maniculatus (17 isolates), tamias minimus (11 isolates), spermophilus lateralis (3 isolates), and spermophilus beechey ... | 2003 | 12574261 |
| comparison of pcr assays for detection of the agent of human granulocytic ehrlichiosis, anaplasma phagocytophilum. | human granulocytic ehrlichiosis is an emerging infectious disease in the united states and europe, and pcr methods have been shown to be effective for the diagnosis of acute infections. numerous pcr assays and primer sets have been reported in the literature. the analytical sensitivities (limits of detection) of 13 published pcr primer sets were compared using dna extracted from serial dilutions of anaplasma phagocytophilum-infected hl-60 cells. the specificity of the assays that were able to de ... | 2003 | 12574272 |
| molecular diagnosis of infective endocarditis by pcr amplification and direct sequencing of dna from valve tissue. | we used broad-range eubacterial pcr amplification followed by direct sequencing to identify microbial pathogens in heart valve material from 29 patients with histologically confirmed infective endocarditis and 23 patients free of infective endocarditis. microorganisms cultured by conventional techniques matched those identified by pcr in 21 cases. pcr alone identified the causative agent in three cases (streptococcus bovis, staphylococcus cohnii, and coxiella burnetii), allowing better patient m ... | 2003 | 12574279 |
| characterization of the cryptic plasmid pbgr1 from bartonella grahamii and construction of a versatile escherichia coli-bartonella spp. shuttle cloning vector. | we report herein the isolation and molecular characterization of pbgr1, the first native plasmid isolated from the genus bartonella. cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. the regions containing rep and mob are separated by 140-bp inverted repeat sequences and display a difference in g + c content from one another. a 1435-bp saci-bcli fragment containing the rep gene is su ... | 2003 | 12584000 |
| blood culture negative endocarditis: analysis of 63 cases presenting over 25 years. | to analyse cases of blood culture negative endocarditis (bcne) seen at st thomas' hospital, london, between 1975 and 2000. | 2003 | 12591823 |
| hemin binding, functional expression, and complementation analysis of pap 31 from bartonella henselae. | growth of bartonella henselae is strongly heme dependent, and b. henselae is unable to synthesize heme itself. at least five outer membrane-associated proteins from b. henselae bind hemin, including the 31-kda protein designated pap31. the gene of this protein was heterologously expressed in escherichia coli m15(prep4) and detected with monoclonal antibodies in the outer membrane fraction. complementation of the hema-deficient mutant e. coli k-12 eb53 (arob tsx malt hema) with pap31 demonstrated ... | 2003 | 12591895 |
| diffusion-weighted magnetic resonance imaging abnormalities in bartonella encephalopathy. | the authors describe 2 patients with new-onset, refractory status epilepticus and serological evidence for bartonella infection. brain magnetic resonance imaging (mri) in patient 1 showed transient diffusion abnormalities in the posterior (pulvinar) thalami. in patient 2, brain mri showed several enhancing cortical lesions, of which one lesion was bright on diffusion-weighted imaging (dwi). in patients with unexplained, refractory seizures, the presence of dwi abnormalities warrants a search for ... | 2003 | 12593136 |
| clinical quiz: cat scratch disease. | 2003 | 12593397 | |
| unusual presentation of cat scratch disease in hiv+ patient. | 2003 | 12598467 | |
| bartonella quintana bacteremia and overproduction of interleukin-10: model of bacterial persistence in homeless people. | chronic asymptomatic bacteremia caused by bartonella quintana occurs in homeless people, but its pathophysiology is unknown. we investigated homeless people with bacteremia to determine whether the persistence of b. quintana is associated with a specific immune profile. homeless people without b. quintana infection exhibited an inflammatory profile--levels of circulating markers of leukocyte activation (soluble interleukin [il]-2 receptor and neopterin) and cytokines released by mononuclear cell ... | 2003 | 12599058 |
| [hepatosplenic localization of cat scratch disease in immunocompetent adults. two cases]. | the infective agent responsible for cat scratch disease, bartonella henselae, is a rare cause of hepatic granulomatosis in immunocompetent adults. clinical features include a prolonged fever or more typical symptoms such as lymphadenopathy associated with painful hepatomegaly and a fever following a cat scratch or bite. images of micronodular hepatosplenic lesions on abdominal ultrasonography or computed tomography scan along with epithelioid granulomas in a liver biopsy can suggest this diagnos ... | 2003 | 12658133 |
| chlamydia pneumoniae, but not bartonella quintana, is associated with coronary heart disease: results of a french case-control study. | serologic cross-reactivity has been demonstrated between bartonella quintana and chlamydia pneumoniae. therefore, the association between antibodies to c. pneumoniae and coronary heart disease (chd) as described in the literature may be due to antibodies cross-reacting with b. quintana. to investigate this hypothesis, we evaluated, in a case-control study, the prevalence of c. pneumoniae and b. quintana antibodies among 296 cases with angiographically significant artery lesions and 170 controls ... | 2003 | 12667243 |
| cure of bartonella endocarditis of a prosthetic aortic valve without surgery: value of serologic follow-up. | bartonella species are emerging as an important cause of blood culture-negative endocarditis, but the optimal management of this disease has not been fully defined. we describe a case of subacute bartonella henselae endocarditis of a prosthetic aortic valve in an immunocompetent woman that was cured with long-term antibiotic therapy alone. in addition, we demonstrate that follow-up of serologic titers against b. henselae was helpful in assessing definitive cure of the infection. | 2003 | 12667258 |
| differential expression of the invasion-associated locus b (ialb) gene of bartonella bacilliformis in response to environmental cues. | bartonella bacilliformis is the causative agent of the biphasic human disease, oroya fever. during the primary disease phase, up to 100% of the circulating erythrocytes can be parasitized and 80% lysed. during the secondary phase of this disease, bacterial invasion shifts to endothelial cells lining the vasculature. b. bacilliformis is transferred between human hosts by the sandfly, lutzomyia verrucarum. to investigate the regulation of ialb by environmental cues signaling vector-to-host transmi ... | 2003 | 12668141 |
| primary lesion of cat-scratch disease and inguinal cat-scratch disease. | 2003 | 12674683 | |
| [bartonellosis]. | in the last years the number of bartonella species significantly raised, often with an epidemiological profile of emergent disease. b. bacilliformis is the etiological agent of carrion's disease. b. clarridgeiae has been associated, together with b. henselae, to cat-scratch disease (csd), whereas b. elizabethae to endocarditis and b. grahamii to neuroretinitis. b. henselae has been associated to csd and, in patients with immunodeficiency, together with b. quintana, to bacillary angiomatosis, to ... | 2003 | 12677790 |
| prevalence of bartonella henselae in young, healthy cats in sweden. | 2003 | 12678262 | |
| seroprevalence of bartonella henselae, toxoplasma gondii, fiv and felv infections in domestic cats in japan. | seroprevalence of bartonella henselae, toxoplasma gondii, feline immunodeficiency virus (fiv) and feline leukemia virus (felv) infections was investigated in 1,447 domestic cats derived from the north (hokkaido) to the south (okinawa) prefectures in japan. of the cats investigated, 8.8% (128/1,447) were seropositive to b. henselae, 5.4% (78/1,447) to t. gondii, 9.8% (107/1,088) to fiv, and 2.9% (32/1,088) to felv, respectively. for b. henselae infection, the positive rate varied from 11.5% in ca ... | 2003 | 12680718 |
| [transverse myelitis associated with cat-scratch disease]. | 2003 | 12681227 | |
| blood agar and mycobacterium tuberculosis: the end of a dogma. | incidental blood agar-based recovery of mycobacterium tuberculosis led us to further investigate this routine medium for primary isolation and culture of m. tuberculosis. fifteen respiratory tract and eight lymph node ziehl-neelsen-positive specimens were inoculated in parallel into tubes containing egg-based medium and 5% sheep blood agar. colonies appeared sooner on this medium than on the egg-based medium, but this difference was not significant (p = 0.11, analysis of variance [anova] test). ... | 2003 | 12682165 |
| characterization of the human autoimmune response to the major c-terminal epitope of the ribosomal p proteins. | autoantibodies to the ribosomal phospho (-p) proteins p0, p1, and p2, collectively referred to as rib-p, are specifically found in 10-40% of patients with systemic lupus erythematosus (sle). these antibodies are believed to be correlated with lupus nephritis, hepatitis, and central nervous system involvement. the major immunoreactive epitope of these ribosomal antigens has been localized to the carboxy terminus, which is a highly conserved domain of all three proteins and contains two phosphoryl ... | 2003 | 12682728 |
| urban trench fever and cat scratch disease. | 2003 | 12684929 | |
| bartonella henselae: association with the development of transient lupus anticoagulant and asymptomatic prolongation of activated partial thromboplastin time. | 2003 | 12693573 | |
| detection and characterization of feline bartonella henselae in the czech republic. | the aims of the study were to characterize isolates of bartonella henselae and to determine the prevalence of bacteremic domestic cats in urban and suburban parts of prague, czech republic. five (18%) gram-negative fastidious bacterial single-cat isolates were recovered from 27 hemocultures incubated without previous freezing. four of these isolates originated from flea infested stray cats (n=6) and one from a shelter cat without any ectoparasites (n=21). none of the 34 previously frozen specime ... | 2003 | 12695049 |
| [systematics of the lutzomyia species of the verrucarum theodor group, 1965 (diptera: psychodiadae)]. | the verrucarum group of phlebotomine sand flies includes vectors of leishmania spp. and bartonella bacilliformis, and from the perspective of public health is considered as one of the most important groups of neotropical phlebotomine sand flies. due to marked morphological similarity among species constituting this group, the identification based on conventional taxonomic characters can be difficult. consequently, the verrucarum group has been the focus of numerous taxonomic comparisons which ha ... | 2003 | 12696402 |
| [kinetics of bartonella spp. igg antibodies in cat-scratch disease and in asymptomatic hiv-infected patients]. | our goal was to investigate the kinetics of serum igg antibodies against bartonella spp. in patients with cat-scratch disease and in hiv-infected indiviuals. | 2003 | 12716542 |
| [non henselae bartonella neuroretinitis]. | 2003 | 12716551 | |
| [possible bacterial etiologies for diseases of unknown origin]. | 2003 | 12717985 | |
| [cat scratch disease]. | 2003 | 12722263 | |
| immune responses of immunocompetent and immunocompromised mice experimentally infected with bartonella henselae. | the aim of this study is to understand host immune responses in immunocompetent and immunocompromised mice against bartonella henselae infection. balb/c and nude (balb/c nu/nu) mice were inoculated intraperitoneally with 10(8) colony forming units of b. henselae (houston-1 strain). blood, brain, liver, spleen, kidney and bone marrow samples were collected 0, 3, 7, 14, 21 and 28 days after infection and submitted to bacteriological, serological and genetical examinations. b. henselae was isolated ... | 2003 | 12736430 |
| a rhizobium leguminosarum acpxl mutant produces lipopolysaccharide lacking 27-hydroxyoctacosanoic acid. | the structure of the lipid a from rhizobium etli and rhizobium leguminosarum lipopolysaccharides (lpss) lacks phosphate and contains a galacturonosyl residue at its 4' position, an acylated 2-aminogluconate in place of the proximal glucosamine, and a very long chain omega-1 hydroxy fatty acid, 27-hydroxyoctacosanoic acid (27ohc28:0). the 27ohc28:0 moiety is common in lipid a's among members of the rhizobiaceae and also among a number of the facultative intracellular pathogens that form chronic i ... | 2003 | 12618448 |
| [three toddlers with a swelling in the neck]. | three children, a girl aged 2.5 years and two boys aged 2 and 3 years respectively, presented with unilateral cervical lymphadenitis. the first patient had acute bacterial lymphadenitis due to group a streptococcus, characterised by a painful cervical swelling of acute onset. the second patient had painless cervical lymphadenitis caused by mycobacterium avium-intracellulare, which drained spontaneously. the third patient developed a non-tender, cervical swelling within a day. he too was systemic ... | 2003 | 12621974 |
| diagnosis of bartonella endocarditis by a real-time nested pcr assay using serum. | bartonella endocarditis is a severe disease for which blood cultures frequently remain negative. we tested three pcr assays by using specimens of serum sampled early during the disease from 43 patients diagnosed in our laboratory as having bartonella endocarditis on the basis of serological, culture, and/or valvular molecular detection. we tested a two-step nested pcr (tsn-pcr), a one-step nested pcr (osn-pcr) with a regular thermal cycler, and a one-step nested pcr with the lightcycler (lcn-pcr ... | 2003 | 12624010 |
| detection and identification of bartonella species pathogenic for humans by pcr amplification targeting the riboflavin synthase gene (ribc). | several bartonella species have now been implicated as human pathogens. the recovery of these fastidious organisms in the clinical microbiology laboratory remains difficult, and current methods are still relatively insensitive. thus, the bartonellae are good candidates for detection by pcr. we have developed a pcr assay which uses a single primer pair targeting the riboflavin synthase gene (ribc) and detected six bartonella species that have been implicated in human disease, b. henselae, b. quin ... | 2003 | 12624031 |
| in search of encephalitis etiologies: diagnostic challenges in the california encephalitis project, 1998-2000. | the california encephalitis project was initiated in june 1998 to identify the causes and characterize the clinical and epidemiologic features of encephalitis in california. testing for >or=13 agents, including herpesviruses, enteroviruses, arboviruses, bartonella species, chlamydia species, and mycoplasma pneumoniae, was performed at the viral and rickettsial disease laboratory (richmond, california). epidemiologic and clinical information collected for each case guided further testing. from ju ... | 2003 | 12627357 |
| phase variation in bartonella henselae. | bartonella henselae is a fastidious, gram-negative bacterial pathogen of cats and humans. previous workers have shown that serial passage in vitro leads to attenuation of virulence-associated attributes such as expression of pili, invasion of human epithelial cell lines and the stimulation of endothelial cell proliferation. in contrast to the published data, it was found that pilin expression is frequently preserved in organisms which have undergone phase variation in vitro. transition from a sl ... | 2003 | 12634331 |
| oral corticosteroids in cat-scratch disease. | 2003 | 12635985 | |
| detection of bartonella henselae dna using polymerase chain reaction assay in patient with cat scratch disease. | 2003 | 12636034 | |
| bartonella henselae in ixodes ricinus ticks (acari: ixodida) removed from humans, belluno province, italy. | the potential role of ticks as vectors of bartonella species has recently been suggested. in this study, we investigated the presence of bartonella species in 271 ticks removed from humans in belluno province, italy. by using primers derived from the 60-kda heat shock protein gene sequences, bartonella dna was amplified and sequenced from four ixodes ricinus ticks (1.48%). to confirm this finding, we performed amplification and partial sequencing of the pap31 protein and the cell division protei ... | 2003 | 12643827 |
| molecular detection of bartonella quintana, b. koehlerae, b. henselae, b. clarridgeiae, rickettsia felis, and wolbachia pipientis in cat fleas, france. | the prevalences of bartonella, rickettsia, and wolbachia were investigated in 309 cat fleas from france by polymerase chain reaction (pcr) assay and sequencing with primers derived from the glta gene for rickettsia, the its and pap31 genes for bartonella, and the 16s rrna gene for anaplasmataceae. positive pcr results were confirmed by using the lightcycler and specific primers for the rompb of rickettsia and glta of bartonella. r. felis was detected in 25 fleas (8.1%), w. pipientis, an insect s ... | 2003 | 12643829 |
| [cat scratch disease with posterior segment involvement]. | to describe the clinical characteristics of patients with cat scratch disease during the last 2 years. | 2003 | 12647335 |
| bartonella infection in sylvatic small mammals of central sweden. | sylvatic small mammals were captured in rural habitats near uppsala, sweden, to measure the prevalence of bartonella infections, characterize bacterial isolates and identify their host range, and increase our understanding of host-pathogen ecology. during 7 nights of trapping at 3 localities, 236 small mammals were captured (trap success 30%). bartonella were isolated from bloods of apodemus flavicollis (19 of 110 tested), apodemus sylvaticus (6/25), clethrionomys glareolus (9/60), microtus agre ... | 2003 | 12613756 |
| in vitro bartonella quintana infection modulates the programmed cell death and inflammatory reaction of endothelial cells. | bartonella quintana is an epicellular bacterium, which in vivo as well as in vitro, invades endothelial cells and develops within them inducing proliferative effects that play a pivotal role in neovascular manifestation of this disease. we investigated the effect of live bartonella quintana and its lps on apoptosis and inflammatory response in huvec-c, an endothelial cell line. the kinetics of the programmed cell death of bartonella quintana-infected huvec-c showed a peculiar course. even if ear ... | 2003 | 12614981 |
| type iv transporters of pathogenic bacteria. | type iv transporters are produced by several bacterial pathogens such as agrobacterium tumefaciens, bordetella pertussis, brucella spp., bartonella henselae, helicobacter pylori and legionella pneumophila. these transporters are critical for the pathogenic process in that they export important virulence factors across the membranes of the bacteria. although the virulence factors that are exported by these transporters can be either nucleic acid or protein, the general mechanism of transport appe ... | 2003 | 12615216 |
| recent progress in understanding bartonella-induced vascular proliferation. | the ability to induce endothelial cell proliferation is a common feature of human pathogenic bartonella species. recent data have indicated that bartonellae can provoke angioproliferation by at least two independent mechanisms: directly, by triggering proliferation and inhibiting apoptosis of endothelial cells; and indirectly, by stimulating a paracrine angiogenic loop of vascular endothelial growth factor production by infected macrophages. a nf-kappab-mediated acute inflammatory reaction of th ... | 2003 | 12615221 |
| rapid polymerase chain reaction-based confirmation of cat scratch disease and bartonella henselae infection. | cat scratch disease (csd) commonly occurs secondary to bartonella henselae infection, and the diagnosis has traditionally been made by microscopic findings, the identification of organisms by cytochemistry, and clinical history. however, cytochemical analysis tends to be very difficult to interpret, and histology alone may be insufficient to establish a definitive diagnosis of csd. | 2003 | 12741894 |
| [severe retinal phlebitis in ocular bartonellosis]. | purpose/methods: to report the clinical case of a 34-year-old male with atypical ophthalmic manifestations of cat-scratch disease (ocular bartonellosis), including an extensive retinal phlebitis, as well as the evolution of the clinical picture with treatment. results/conclusions: the clinical diagnosis of ocular bartonellosis was serologically confirmed. oral treatment with ciprofloxacine healed the phlebitis and the neuroretinitis, with a residual optic disk pallor. this case exemplifies the d ... | 2003 | 12743848 |
| monoclonal and biclonal gammopathy in two patients infected with bartonella henselae. | two immunocompetent patients with cat-scratch disease due to infection with bartonella henselae developed monoclonal and biclonal gammopathy. neither patient had evidence of any other known cause of plasma cell dyscrasia, and antibiotic eradication of bartonella henselae infection resulted in the prompt disappearance of the gammopathy. hence, cat-scratch disease should be added to the list of possible underlying disorders in individuals presenting with monoclonal and biclonal gammopathy. | 2003 | 12764550 |
| lymphadenopathy at the medial epitrochlear region in cat-scratch disease. | our objective was to describe the imaging features of lymphadenopathy at the medial epitrochlear region as frequently seen in cat-scratch disease (csd) and to compare these features with those seen in non-csd. ten patients (four males and six females) presented with a subcutaneous soft tissue mass at the medial epitrochlear region. three patients were younger than 15 years. magnetic resonance imaging was performed in all patients. all lesions turned out to be regional lymphadenopathy instead of ... | 2003 | 12764654 |
| what do we (not) know about the human bartonelloses? | the human bartonelloses are a group of diseases with a rapidly increasing clinical spectrum. well known manifestations such as carrion's disease, trench fever, cat-scratch disease, and bacillary angiomatosis are examples of bartonella sp. infection. along with these diseases, recurrent bacteremia, endocarditis, septicemia, erythema nodosum, erythema multiforme, trombocytopenic purpura and other syndromes have been reported having been caused by bacteria of this genus. the infectious process and ... | 2003 | 12807686 |
| occurrence of bartonella henselae and bartonella quintana in a healthy greek population. | the purpose of this study was to determine the prevalence of igm and igg antibodies against bartonella henselae and b. quintana in a healthy greek population using a commercially available immunofluorescent test (focus test). five hundred healthy individuals were divided by sex into four age groups and three groups according to contact with cats. igm antibodies were not detected in any of the subjects examined, while 99 (19.8%) and 75 (15%) were igg seropositive to b. henselae and to b. quintana ... | 2003 | 12812344 |
| randomized open trial of gentamicin and doxycycline for eradication of bartonella quintana from blood in patients with chronic bacteremia. | chronic bartonella quintana bacteremia is known to occur in homeless people exposed to lice. we present here the results of an open randomized trial performed to evaluate the efficacy of doxycycline in combination with gentamicin in the eradication of b. quintana bacteremia. from 1 january 2001 to 1 april 2002, homeless people with blood cultures positive for b. quintana were randomized to receive either no treatment (untreated controls) or a combination of gentamicin (3 mg/kg of body weight/day ... | 2003 | 12821469 |
| cloning and characterization of an outer membrane protein (lip18) from helicobacter bizzozeronii. | a recombinant lambda-zap ii phage was selected by screening a genomic library of helicobacter bizzozeronii (hb) using antibodies from a naturally infected cat. dna sequencing resulted an open reading frame containing 172 codons with a predicted molecular mass of 18 kda (lip18). the amino acid sequence showed 22.1, 55.2, 56.7 and 57.1% identity to peptidoglycan-associated lipoprotein of helicobacter pylori, agrobacterium tumefaciens, bartonella bacilliformis, respectively. a peptidoglycan associa ... | 2003 | 12825349 |
| a case of cat scratch disease neuroretinitis confirmed by polymerase chain reaction. | cat scratch disease neuroretinitis is caused by infection by bartonella henselae. to demonstrate b. henselae infection, serologic examination is commonly used, but sometimes serologic examination is not adequate for correct diagnosis. here we present a case of cat scratch disease neuroretinitis confirmed by polymerase chain reaction in addition to serologic examination. | 2003 | 12842212 |
| osteomyelitis caused by bartonella henselae genotype i in an immunocompetent adult woman. | we report the first description of osteomyelitis due to bartonella henselae genotype i in an immunocompetent middle-aged woman. the diagnosis was established by serology, histopathology, and pcr analysis of osseous and lymph node tissues. the mycobacteria growth indicator tube inoculated with the lymph node aspirate was used for pcr analysis. | 2003 | 12843112 |
| detection by immunofluorescence assay of bartonella henselae in lymph nodes from patients with cat scratch disease. | laboratory diagnosis of bartonella henselae infections can be accomplished by serology or pcr assay on biopsy samples. the purpose of our work was to assess immunofluorescence detection (ifd) in lymph node smears using a specific monoclonal antibody directed against b. henselae and a commercial serology assay (ifa) compared with pcr detection. among 200 lymph nodes examined from immunocompetent patients, 54 were positive for b. henselae by pcr, of which 43 were also positive by ifd. among the 14 ... | 2003 | 12853405 |
| identification of rickettsia spp. and bartonella spp. in ffrom the thai-myanmar border. | during a survey for possible rickettsial vectors in villages of the central part of the thai-myanmar border from september 2001 to february 2002, four species of fleas were collected from common peridomestic animals. all fleas were tested by pcr to detect dna of bacteria of the genera rickettsia (glta and ompb genes) and bartonella (its and ftsz genes). sequencing of pcr-amplified products was done using glta fragments for rickettsia and ftsz fragments for bartonella: two genotypes related to ri ... | 2003 | 12860622 |
| bartonella grahamii infecting rodents display high genetic diversity over short geographic distances. | 2003 | 12860632 | |
| bartonella schoenbuchensis isolated from the blood of a french cow. | 2003 | 12860633 | |
| clinical impact of persistent bartonella bacteremia in humans and animals. | bartonella spp. are emerging vector-borne pathogens that cause persistent, often asymptomatic bacteremia in their natural hosts. as our knowledge progresses, it appears that chronic infection may actually predispose the host to mild, insidious nonspecific manifestations or induce, in selected instances, severe diseases. persistent asymptomatic bacteremia is most common in animals that serve as the main reservoir for the specific bartonella. in humans, these organisms are b. bacilliformis and b. ... | 2003 | 12860639 |
| real-time pcr duplex assay for rickettsia prowazekii and borrelia recurrentis. | rickettsia prowazekii, the etiologic agent for epidemic typhus, and borrelia recurrentis, the etiologic agent of relapsing fever, both utilize the same vector, the human body louse (pediculus humanus), to transmit human disease. we have developed an assay to detect both bacterial pathogens in a single tube utilizing real-time pcr. assays for both agents are specific. the r. prowazekii and b. recurrentis assays do not detect nucleic acid from r. typhi, r. canada, or any of eight spotted fever ric ... | 2003 | 12860643 |
| genetic variation of bartonella henselae detected in lymph nodes from patients with cat scratch disease in slovenia. | 2003 | 12860661 | |
| cat scratch disease with lymphadenitis, vertebral osteomyelitis, and spleen abscesses. | in this report we describe a 30-year old male patient with vertebral osteomyelitis and spleen abscesses with cat scratch disease. the diagnosis was made on the basis of molecular detection of bartonella henselae either on lymph node biopsies or on bone biopsy, histology of the lymph node, serology using either our in-house microimmunofluorescence assay or a commercial kit (focus technologies). immunofluorescent detection was also performed directly on slide appositions using a monoclonal antibod ... | 2003 | 12860662 |
| bartonella henselae and borrelia burgdorferi infections of the central nervous system. | to investigate the role of b. henselae in patients with symptoms suggesting neuroborreliosis, serum and cerebrospinal fluid samples were tested with serological and pcr methods. among 17 examined patients, in 12 cases borrelia burgdorferi infections were detected, in 1 case bartonella henselae infection was ascertained, and in two patients mixed b. burgdorferi and b. henselae infections were found. these results indicate that mixed infections should be taken into consideration in establishing di ... | 2003 | 12860663 |
| seroprevalence of bartonella henselae and bartonella quintana infections in poland in 1998-2001. | 2003 | 12860664 | |
| bartonella spp. antibodies in forensic samples from swedish heroin addicts. | a high frequency of bartonella elizabethae seropositivity (39%) was recorded among intravenous heroin addicts in stockholm, sweden, who died from a lethal injection. some of the b. elizabethae-seropositive individuals also had antibodies to b. henselae houston-1, b. grahamii, and b. quintana, but none had antibodies to b. henselae marseille or b. vinsonii subsp. vinsonii. hepatitis was a frequent finding but no case had peliosis hepatitis. there was no case of endocarditis, but in three persons ... | 2003 | 12860665 |
| prevalence of bartonella infections in slovenian intravenous drug users. | 2003 | 12860666 | |
| erythroblast cells as a target for bartonella quintana in homeless people. | 2003 | 12860677 | |
| immunofluorescence detection of bartonella bacilliformis flagella in vitro and in vivo in human red blood cells as viewed by laser confocal microscopy. | 2003 | 12860693 | |
| studies on the identification of deforming factor from bartonella bacilliformis. | 2003 | 12860696 | |
| experimental infection of human erythrocytes from alcoholic patients with bartonella quintana. | bartonella spp. are found in the erythrocytes of their specific natural hosts and b. quintana bacteremia is associated epidemiologically with lice, alcoholism, and homelessness. the aim of our study was to compare the growth and the number of bacteria per erythrocyte in vitro in laboratory-infected red blood cells from alcoholic patients versus normal blood donor erythrocytes. enumeration of bacteria was performed either with plate counting or with a real-time pcr quantitative assay. number of b ... | 2003 | 12860697 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 22-2003. a 22-year-old man with chills and fever after a stay in south america. | 2003 | 12867613 | |
| characterization of new members of the group 3 outer membrane protein family of brucella spp. | impairment of the omp25 gene in brucella spp. leads to attenuated strains and confers protection to the host. omp25 and omp31, whose functions remain unknown, were the first characterized members of group 3 outer membrane proteins (omps) (25 to 34 kda). recently, genomic and proteomic approaches identified five new putative members of this family, some of which are produced in b. melitensis or b. abortus. in the present study, using protein microsequencing, we identified new members of group 3 o ... | 2003 | 12874309 |
| molecular characterization of the sucb gene encoding the immunogenic dihydrolipoamide succinyltransferase protein of bartonella vinsonii subsp. berkhoffii and bartonella quintana. | members of the genus bartonella have historically been connected with human disease, such as cat scratch disease, trench fever, and carrion's disease, and recently have been recognized as emerging pathogens causing other clinical manifestations in humans. however, because little is known about the antigens that elicit antibody production in response to bartonella infections, this project was undertaken to identify and molecularly characterize these immunogens. immunologic screening of a bartonel ... | 2003 | 12874367 |
| diagnostic methods. current best practices and guidelines for identification of difficult-to-culture pathogens in infective endocarditis. | ie is a serious, life-threatening disease. because treatment must often be adapted to the pathogen involved, rapid identification of the etiologic agent is critical to successful management of each patient. when difficult-to-culture pathogens are involved, routine microbiologic tests, including blood culture, may remain negative. because such cases may account for up to 31% of all ie cases, alternative diagnostic approaches are necessary. among the etiologic agents of culture-negative endocardit ... | 2003 | 12874894 |
| first case in bulgaria of parinaud's oculoglandular syndrome associated with bartonella henselae. | 2003 | 12875532 | |
| gene-sequence-based criteria for species definition in bacteriology: the bartonella paradigm. | the definition of new species is currently based on polyphasic classification that includes both determination of phenotypic characteristics and dna-dna homology. however, none of these techniques is convenient for the rapid characterization of fastidious or non-culturable bacteria. using sequences available in the genbank database, we compared the similarities of gene fragments among the currently recognized bartonella species. this comparison led to both the definition of similarity values tha ... | 2003 | 12875815 |
| upper gastrointestinal bacillary angiomatosis causing hematemesis: a case report. | a 38-year-old hiv-positive woman presented with massive hematemesis on initial admission to hospital. endoscopy revealed ulcerated nodular lesions in the esophagus, stomach, and duodenum. the clinical impression was of kaposi's sarcoma. the stomach was biopsied when the patient re-presented, and another endoscopy was performed. the biopsy showed mucosal ulceration with a proliferation of vascular channels associated with neutrophils and clumps of purplish, granular bacterial colonies, which were ... | 2003 | 12894361 |
| cat scratch disease presenting as orbital abscess and osteomyelitis. | ocular manifestations of cat scratch disease are uncommon. the diagnosis is usually made on the basis of increasing bartonella henselae serum antibody titers. we report a child presenting with orbital abscess and osteomyelitis who was diagnosed with hepatosplenic cat scratch disease by detection of b. henselae dna in the orbital abscess fluid. | 2003 | 12904438 |
| first isolation and detection by immunofluorescence assay of bartonella koehlerae in erythrocytes from a french cat. | 2003 | 12904442 | |
| prevalence of bartonella infection among human immunodeficiency virus-infected patients with fever. | bartonella infection can be difficult to diagnose, especially when it manifests as bacteremia, which is usually accompanied by nonspecific symptoms, such as fever. therefore, we hypothesized that bartonella infection represents an underrecognized cause of febrile illness. to determine the prevalence of bartonella infection among patients presenting with fever, we evaluated 382 patients in san francisco. overall, 68 patients (18%) had evidence of bartonella infection detected by culture, indirect ... | 2003 | 12905141 |
| molecular and immunological approaches to controlling parasitic infection. | 2003 | 12906731 | |
| the role of the host immune response in pathogenesis of bartonella henselae. | bartonella henselae can infect humans resulting in a wide range of disease syndromes including cat-scratch disease, fever with bacteremia, endocarditis, bacillary angiomatosis, and bacillary peliosis hepatis, among others. the nature and severity of the clinical presentation correlates well with the status of the hosts' immune system. individuals with impaired immune function, including hiv infection, progress to systemic infections more often. patients with intact immune function who become inf ... | 2003 | 12906736 |
| bartonellosis. | the role of bartonella species as pathogens in dogs and cats is being defined. diagnosis and treatment of bartonella infections of dogs and cats remain challenging. as new information regarding bartonella infections of companion animals becomes available, the understanding of the pathogenesis of these infections will improve. most bartonella species infecting dogs and cats are zoonotic, with b henselae the most important zoonotic species. b henselae bacteremia is common in domestic cats, and cat ... | 2003 | 12910745 |
| canine borreliosis. | a guild of organisms carried by the same vector (ixodes ticks) in lyme-endemic areas may be confounding the understanding of lyme disease in dogs. a new diagnostic method, the c6 peptide test for lyme, and serology and pcr testing for ehrlichia, babesia, and bartonella species will help to sort out seroprevalence and symptomatology caused by exposure to these agents or by coinfections. in addition, rickettsia, leptospira, mycoplasma species, and more could be involved in dogs diagnosed with a "d ... | 2003 | 12910746 |
| the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis. | bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ... | 2003 | 12918816 |
| a bacterial conjugation machinery recruited for pathogenesis. | type iv secretion systems (t4ss) are multicomponent transporters of gram-negative bacteria adapted to functions as diverse as dna transfer in bacterial conjugation or the delivery of effector proteins into eukaryotic target cells in pathogenesis. the generally modest sequence conservation between t4ss may reflect their evolutionary distance and/or functional divergence. here, we show that the establishment of intraerythrocytic parasitism by bartonella tribocorum requires a putative t4ss, which s ... | 2003 | 12940985 |
| molecular cloning and analysis of a region of the bartonella bacilliformis genome encoding nlpd, l-isoaspartyl methyltransferase and yajc homologs. | the nlpd/lppb homolog of the human pathogen, bartonella bacilliformis, is an immunogenic 43-kda protein that is encoded by a 1206-bp open reading frame (orf-401). the regions flanking the nlpd/lppb gene of b. bacilliformis were sequenced to determine if it is located within the rpos operon, as it is in most bacteria. we report that the b. bacilliformis nlpd/lppb gene is located immediately downstream of pcm, a gene encoding a 25-kda protein, l-isoaspartyl protein carboxyl methyltransferase, that ... | 2003 | 12941162 |
| detection of novel bartonella strains and yersinia pestis in prairie dogs and their fleas (siphonaptera: ceratophyllidae and pulicidae) using multiplex polymerase chain reaction. | we developed a multiplex polymerase chain reaction (pcr) assay that simultaneously detects three types of flea-associated microorganisms. targets for the assay were sequences encoding portions of the glta, a 17-kda antigen, and pla genes of bartonella spp. strong et al., rickettsia spp. da rocha-lima, and yersinia pestis yersin, respectively. a total of 260 flea samples containing bloodmeal remnants were analyzed from fleas collected from abandoned prairie dog (cynomys ludovicianus) burrows at t ... | 2003 | 12943112 |
| [bilateral retinal artery branch occlusions revealing bartonella grahamii infection]. | 2003 | 12951187 | |
| isolated splenic cat scratch disease. | 2003 | 12955654 | |
| rapid and efficient transposon mutagenesis of bartonella henselae by transposome technology. | molecular genetics are difficult to perform in bartonella henselae, the causative agent of cat scratch disease and the vasculoproliferative disorders bacillary angiomatosis and bacillary peliosis. to elucidate the underlying bacterial pathogenic mechanisms, genetic manipulation of b. henselae is the method of choice. we describe how to perform transposon mutagenesis in b. henselae using transposome technology. b. henselae mutants revealed by this technique showed random transpositional insertion ... | 2003 | 12957381 |
| a study on forensic samples of bartonella spp antibodies in swedish intravenous heroin addicts. | infection with bartonella, an emerging bacterial pathogen which often affects immunodeficient patients, has been reported in sweden over the past few years, with a high seroprevalence of b. elizabethae. a high prevalence of antibodies against b. elizabethae has also been found in urban intravenous drug users in the usa. using immunofluorescence, we retrospectively examined serum samples taken at autopsy from 59 swedish intravenous drug addicts from the stockholm area for evidence of antibodies a ... | 2003 | 12780526 |
| asking the right question. | 2003 | 12781538 | |
| bartonella quintana lipopolysaccharide effects on leukocytes, cxc chemokines and apoptosis: a study on the human whole blood and a rat model. | bartonella quintana, an emerging gram-negative pathogen, may cause trench fever, endocarditis, cerebral abscess and bacillary angiomatosis usually with the absence of septic shock in humans. b. quintana lipopolysaccharide (lps), a deep rough endotoxin with strong reactivity in the limulus amebocyte lysate (lal)-assay, was studied in human whole blood and in a rat model. a significant (p<0.05) increase of interleukin-8 (il-8) concentration, comparable to the level induced by enterobacterial lps, ... | 2003 | 12781702 |
| cat-scratch disease occurring in three siblings simultaneously. | we report for the first time three siblings with cat-scratch disease occurring simultaneously. case 1 was an 8-year-old boy with enlarged supraclavicular lymph nodes and fever. case 2 was the 11-year-old sister whose presentation was a prolonged febrile illness. case 3 was a 13-year-old male adolescent with adenitis of the neck and thigh. all three patients were treated with rifampin with improvement of their symptoms. | 2003 | 12797315 |
| bartonella quintana and mycobacterium tuberculosis coinfection in an hiv-infected patient with lymphadenitis. | cat scratch disease (csd) is usually associated with bartonella henselae infection in patients with a history of cat exposure, but bartonella quintana may also be a cause of chronic lympadenopathy in patients with cat or flea contact. the lymph node histopathology of csd and tuberculosis may be indistinguishable. we report herein the first description of lymph node coinfection with b. quintana and m. tuberculosis in a 32-year hiv-infected woman. culture of lymph node biopsy material on columbia ... | 2003 | 12799150 |
| characterization of the ftsz gene from ehrlichia chaffeensis, anaplasma phagocytophilum, and rickettsia rickettsii, and use as a differential pcr target. | degenerate primers corresponding to highly conserved regions of previously characterized ftsz genes were used to pcr amplify a portion of the ftsz gene from the genomic dna of ehrlichia chaffeensis (ftsz(ech)), anaplasma phagocytophilum (ftsz(ap)), and rickettsia rickettsii (ftsz(rr)). genome walking was then used to amplify the 5' and 3' termini of the genes. the dna sequences of the resulting amplification products yielded open reading frames coding for proteins with molecular masses of 42.0, ... | 2003 | 12804116 |
| epidemiology and impact of coinfections acquired from ixodes ticks. | ixodes scapularis and other ticks in the ixodes ricinus complex may transmit multiple pathogens, but research on coinfections has been limited. coinfections occur with varying frequency in ticks, but single infections are more common than dual infections. the proportion of i. scapularis or i. ricinus ticks coinfected with both borrelia burgdorferi sensu lato and anaplasma phagocytophila is generally low, ranging from < 1% to 6% in six geographic areas. a higher prevalence of tick coinfection (26 ... | 2002 | 12804168 |
| submacular exudates with serous retinal detachment caused by cat scratch disease. | to present submacular exudates as a manifestation of cat scratch disease. | 2002 | 12778351 |