Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| in vitro selection of resistance in escherichia coli and klebsiella spp. at in vivo fluoroquinolone concentrations. | fluoroquinolones are potent antimicrobial agents used for the treatment of a wide variety of community- and nosocomial- infections. however, resistance to fluoroquinolones in enterobacteriaceae is increasingly reported. studies assessing the ability of fluoroquinolones to select for resistance have often used antimicrobial concentrations quite different from those actually acquired at the site of infection. the present study compared the ability to select for resistance of levofloxacin, ciproflo ... | 2010 | 20409341 |
| identification of a blavim-4 gene in the internationally successful klebsiella pneumoniae st11 clone and in a klebsiella oxytoca strain in hungary. | 2010 | 20410063 | |
| a membrane-located glycosyltransferase complex required for biosynthesis of the d-galactan i lipopolysaccharide o antigen in klebsiella pneumoniae. | d-galactan i is a polysaccharide with the disaccharide repeat unit structure [-->3-beta-d-galf-(1-->3)-alpha-d-galp-(1-->]. this glycan represents the lipopolysaccharide o antigen found in many gram-negative bacteria, including several klebsiella pneumoniae o serotypes. the polysaccharide is synthesized in the cytoplasm prior to its export via an atp-binding cassette transporter. sequence analysis predicts three galactosyltransferases in the d-galactan i genetic locus. they are wbbo (belonging t ... | 2010 | 20410291 |
| pathogenicity of aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis to brown tree frogs (litoria ewingii). | bacterial dermatosepticemia, a systemic infectious bacterial disease of frogs, can be caused by several opportunistic gram-negative bacterial species including aeromonas hydrophila, chryseobacterium indologenes, chryseobacterium meningosepticum, citrobacter freundii, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, and serratia liquifaciens. here we determined the pathogenicity of 3 bacterial species (aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis) associated ... | 2010 | 20412685 |
| [bacterial meningitis due to gram-negative bacilli in adults]. | gram-negative bacilli are causative agents of bacterial meningitis in the neonatal period but rarely cause meningitis in adults without risk factors. | 2010 | 20414871 |
| in vitro assessment of antibacterial activity and cytocompatibility of silver-containing phbv nanofibrous scaffolds for tissue engineering. | infections with bacteria have become a serious problem in joint arthroplasty. this study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) nanofibers loaded with metallic silver particles of a size of 5-13 nm. in vitro antibacterial activity against staphylococcus aureus and klebsiella pneumoniae was studied by microplate proliferation tests. the adhesion, viability, and proliferation properties of fibroblasts (n ... | 2010 | 20415469 |
| [characteristics of urinary tract infections in different patient subpopulations and depending on the bladder emptying system]. | to analyze the prevalence and etiology of urinary tract infection in patients with neurogenic bladder depending on the bladder emptying system used as compared to a population with no neurological impairment. to assess perception of urinary tract infection by patients with neurogenic bladder. | 2010 | 20416242 |
| production and immunogenicity of recombinant ferric enterobactin protein (fepa). | ferric siderophore complexes are produced by most bacteria to acquire iron, a vital element. these complexes are transported across the outer membrane by receptor proteins commonly known as fepa (ferric enterobactin protein). in this study we attempted to evaluate the immunogenicity of the membrane protein fepa, aiming at inhibition of iron uptake to protect invasion of the host by the bacterium. | 2010 | 20418143 |
| abolition of biofilm formation in urinary tract escherichia coli and klebsiella isolates by metal interference through competition for fur. | bacterial biofilms are associated with a large number of persistent and chronic infections. biofilm-dwelling bacteria are particularly resistant to antibiotics and immune defenses, which makes it hard if not impossible to eradicate biofilm-associated infections. in the urinary tract, free iron is strictly limited but is critical for bacterial growth. biofilm-associated escherichia coli cells are particularly desperate for iron. an attractive way of inhibiting biofilm formation is to fool the bac ... | 2010 | 20418434 |
| invasive bacterial infections in neonates and young infants born outside hospital admitted to a rural hospital in kenya. | bacterial sepsis is thought to be a major cause of young infant deaths in low-income countries, but there are few precise estimates of its burden or causes. we studied invasive bacterial infections (ibis) in young infants, born at home or in first-level health units ("outborn") who were admitted to a kenyan rural district hospital during an 8-year period. | 2010 | 20418799 |
| assessing the emergence of resistance: the absence of biological cost in vivo may compromise fosfomycin treatments for p. aeruginosa infections. | fosfomycin is a cell wall inhibitor used efficiently to treat uncomplicated urinary tract and gastrointestinal infections. a very convenient feature of fosfomycin, among others, is that although the expected frequency of resistant mutants is high, the biological cost associated with mutation impedes an effective growth rate, and bacteria cannot offset the obstacles posed by host defenses or compete with sensitive bacteria. due to the current scarcity of new antibiotics, fosfomycin has been propo ... | 2010 | 20419114 |
| electricity generation from glucose by a klebsiella sp. in microbial fuel cells. | as electrochemically active bacteria play an important role in microbial fuel cells (mfcs), it is necessary to get a comprehensive understanding of their electrogenesis mechanisms. in this study, a new electrochemically active bacterium, klebsiella sp. me17, was employed into an "h" typed mfc for electrogenesis, with glucose as the electron donor. the maximum power density was 1,209 mw/m2 at a resistance of 340 omega and the maximum current was 1.47 ma. given the original anode medium, fresh med ... | 2010 | 20419297 |
| cross-linking of trans reentrant loops in the na(+)-citrate transporter cits of klebsiella pneumoniae. | the membrane topology model of the na(+)-citrate transporter cits of klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. a split version of cits was constructed to study domain interactions and proximity relationships of the putative reentrant loops. split cits retained 50% transport activity of the wild-type version in membrane vesicles. unspecific cross-linking of the purified complex with glutar ... | 2010 | 20420430 |
| incidence and antimicrobial susceptibility of escherichia coli and klebsiella pneumoniae with extended-spectrum beta-lactamases in community- and hospital-associated intra-abdominal infections in europe: results of the 2008 study for monitoring antimicrobial resistance trends (smart). | from 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (esbl)-positive escherichia coli isolates in european intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (p < 0.001), but not for esbl-positive klebsiella pneumoniae isolates (16.4% to 17.9% [p > 0.05]). hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for e. coli (p < 0.001) and 20.9% versus 5.3%, respectively, for k. pneumoniae ... | 2010 | 20421398 |
| isecp1-mediated transposition and homologous recombination can explain the context of bla(ctx-m-62) linked to qnrb2. | bla(ctx-m-62), a c508t variant of bla(ctx-m-3b), was transferred from klebsiella pneumoniae jie137 on a conjugative plasmid together with a class 1 integron containing the dfra12-gcuf-aada2 cassette array, iscr1, and qnrb2. bla(ctx-m-62) lies between intact and rearranged copies of isecp1 in a configuration that can be explained by a combination of transposition and homologous recombination and which also illustrates the ability of isecp1 to mobilize an adjacent gene as part of transposition uni ... | 2010 | 20421399 |
| scrotal abscess in a newborn: caused by extended-spectrum beta-lactamase-producing klebsiella pneumoniae. | 2010 | 20431174 | |
| dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in klebsiella pneumoniae. | dielectric barrier discharge plasma was used to generate a stable strain of klebsiella pneumoniae (designated to as kp-m2) with improved 1,3-propanediol production. the specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 u mg(-1), respectively, for wild type to 0.67, 14.4 and 1.6 u mg(-1) for kp-m2. the glycerol flux of kp-m2 was redistributed with the flux to the reductive pathway bein ... | 2010 | 20431910 |
| changing etiology of community-acquired bacterial meningitis in adults: a nationwide multicenter study in korea. | epidemiologic data on the etiologic organisms is important for appropriate empirical antibiotic treatment of bacterial meningitis. we identified the etiologies of community-acquired bacterial meningitis in korean adults and the associated epidemiological factors. a retrospective, multicenter nationwide study was carried out. patients 18 years of age or older with community-acquired bacterial meningitis with a confirmed pathogen were enrolled. demographic, clinical, and microbiological data were ... | 2010 | 20432052 |
| [kinetic mechanisms of glycerol dehydrogenase and 1,3-propanediol oxidoreductase from klebsiella pneumoniae]. | the kinetic mechanisms of two key enzymes in the biotransformation of glycerol to 1,3-propanediol (1,3-pd) by klebsiella pneumoniae, glycerol dehydrogenase (gdh) and 1,3-propanediol oxidoreductase (pdor), was characterized. kinetics on initial velocity and product inhibition revealed that gdh and pdor follow an ordered bi-bi sequential mechanism. kinetic models for gdh and pdor showed that the oxidation reaction catalyzed by gdh was the rate-limiting step in coupled enzymatic reaction when the g ... | 2010 | 20432935 |
| approach of empirical antibiotic treatment: analyzing bacterial resistance of community-acquired bacteremia. | nosocomial bacterial resistance has been advanced and well studied in taiwan, but there were few reports describing the antibiotic susceptibility of community-acquired pathogens. through collecting data from those who received blood cultures in emergency department, we presented an epidemiological study to analyze appropriate empirical therapy for the community-acquired bacteremia in southern taiwan. | 2010 | 20434122 |
| culture-dependent and culture-independent characterization of microorganisms associated with aedes aegypti (diptera: culicidae) (l.) and dynamics of bacterial colonization in the midgut. | in this work we show that the lumen of aedes aegypti midgut is highly colonized by bacteria that were identified by culture-dependent and culture-independent methods. rdna sequences obtained were compared with those from genbank and the main bacterial genera identified were: serratia, klebsiella, asaia, bacillus, enterococcus, enterobacter,kluyvera and pantoea. all genera were identified in midgut except enterobacter that was observed only in eggs. asaia and pantoea were also identified in eggs ... | 2010 | 20434424 |
| pharmacodynamic modeling of intravenous antibiotics against gram-negative bacteria collected in the united states. | in the era of escalating antimicrobial resistance, the choice of effective empiric antimicrobial therapy has become considerably more difficult. in an attempt to improve antimicrobial selection, pharmacodynamic modeling that considers the drug, dose, dosing interval, and duration of infusion is increasingly used as a tool to assist in the clinical decision-making process. | 2010 | 20435246 |
| characterization and molecular epidemiology of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae in a tunisian university hospital. | one hundred extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae were recovered from the intensive care unit and the urology ward of the university hospital of sahloul in tunisia between may 2005 and may 2006. the majority of strains showed a high level of resistance to cefotaxime and ceftazidime. double-disk synergy test and e-test strips were used to confirm production of esbls. the molecular analysis revealed that the majority of strains (91%) carried genes encoding ctx-m-15. ... | 2010 | 20438347 |
| antibiotic resistance patterns of bacterial strains isolated from periplaneta americana and musca domestica in tangier, morocco. | flies and cockroaches are two insects in close contact with human beings. they are carriers of human pathogenic bacteria on the external areas of their bodies or in their digestive tracts. this study examines periplaneta americana and musca domestica collected from the residential areas of six districts in tangier, morocco. | 2010 | 20440055 |
| antimicrobial susceptibility patterns of community-acquired uropathogens in tehran, iran. | antibiotic resistance of urinary tract pathogens has increased worldwide. knowledge of the antibiotic resistance patterns of uropathogens in specific geographical locations is an important factor for choosing an appropriate empirical antimicrobial treatment. the aim of this study was to provide information regarding local resistance patterns of urinary pathogens to the commonly used antibiotics in tehran, iran. | 2010 | 20440056 |
| ventilator-associated pneumonia in a tertiary care hospital in india: role of multi-drug resistant pathogens. | ventilator-associated pneumonia (vap) is the most frequent intensive-care-unit (icu)-acquired infection. the aetiology of vap varies with different patient populations and types of icus. | 2010 | 20440059 |
| klebsiella pneumoniae carbapenemase-producing enterobacteriaceae. | carbapenem-resistant enterobacteriaceae, particularly klebsiella pneumoniae, are rapidly emerging as significant health care-associated pathogens. because of the adverse impact that infection caused by these extensively drug-resistant organisms can have on patient outcomes, efforts to prevent further transmission of these pathogens within the health care setting are urgently needed. in this article, the epidemiology, detection, and prevention of carbapenem-resistant enterobacteriaceae are descri ... | 2010 | 20442598 |
| the profile of antibiotics resistance and integrons of extended-spectrum beta-lactamase producing thermotolerant coliforms isolated from the yangtze river basin in chongqing. | the spreading of extended-spectrum beta-lactamases (esbl)-producing thermotolerant coliforms (tc) in the water environment is a threat to human health but little is known about esbl-producing tcs in the yangtze river. we received 319 esbl-producing stains obtained from the chongqing basin and we investigated antibiotic susceptibility, bla gene types and the presence of integrons and gene cassettes. 16.8% of tc isolates were esbl-producing bacteria and bla(tem+ctx-m) was the predominant esbl type ... | 2010 | 20447743 |
| effects of plant growth promoting bacteria and mycorrhizal on capsicum annuum l. var. aviculare ([dierbach] d'arcy and eshbaugh) germination under stressing abiotic conditions. | capsicum annuum var. aviculare to tarahumara and papago indians and farmers of sonora desert is a promising biological and commercial value as a natural resource from arid and semiarid coastal zones. traditionally, apply synthetic fertilizers to compensate for soil nitrogen deficiency. however, indiscriminate use of these fertilizers might increase salinity. the inoculation by plant growth promoting bacteria (pgpb) and arbuscular mycorrhizal fungi (amf) represents an alternative as potential bio ... | 2010 | 20447830 |
| primary klebsiella pneumoniae liver abscess with metastatic spread to lung and eye, a north-european case report of an emerging syndrome. | a syndrome of community acquired liver abscess caused by highly virulent hypermucoviscous, rmpa positive klebsiella pneumoniae strains occurs with high incidence in asia. we here report a case complicated by metastatic infection, to our knowledge, the first one described in northern europe. this and other recently reported cases indicate the emergence of this severe syndrome outside of asia. | 2010 | 20448814 |
| physical, antibacterial and antioxidant properties of chitosan films incorporated with thyme oil for potential wound healing applications. | chitosan films incorporated with thyme oil for potential applications of wound dressing were successfully prepared by solvent casting method. the water vapor permeability, oxygen transmission rate, and mechanical properties of the films were determined. surface and cross-section morphologies and the film thicknesses were determined by scanning electron microscopy (sem). fourier transform infrared (ft-ir) spectroscopy was conducted to determine functional group interactions between the chitosan a ... | 2010 | 20372985 |
| risk factors and outcomes associated with acquisition of colistin-resistant kpc-producing klebsiella pneumoniae: a matched case-control study. | a matched 1:3 case-control study investigated factors predicting colistin-resistant versus colistin-susceptible kpc-producing klebsiella pneumoniae acquisition and its impact on patient outcomes. case patients were more often admitted from other institutions (p = 0.019) and had longer therapy with beta-lactam/beta-lactamase inhibitors (p = 0.002) and higher overall mortality (p = 0.05). all 52 study isolates were clonally related, suggesting horizontal dissemination. none of these parameters ind ... | 2010 | 20375234 |
| biofilm bacterial communities and abundance in a full-scale drinking water distribution system in shanghai. | community diversity and abundance of biofilms from a full-scale drinking water distribution system in shanghai were characterized by denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna sequences and heterotrophic plate count (hpc), respectively. bacteria affiliated to the beta- and gamma-proteobacteria were dominating in both in-situ and hpc-culturable bacterial communities. other bacteria present included members of alphaproteobacteria, bacteroides, actinobacteria, nitrospirae a ... | 2010 | 20375488 |
| mutant prevention concentrations of four carbapenems against gram-negative rods. | we tested the propensities of four carbapenems to select for resistant escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, and acinetobacter baumannii mutants by determining the mutant prevention concentrations (mpcs) for 100 clinical strains with various ss-lactam phenotypes. among the members of the enterobacteriaceae family and a. baumannii strains, the mpc/mic ratios were mostly 2 to 4. in contrast, for p. aeruginosa the mpc/mic ratios were 4 to > or =16. the mpc/mic ratios for ... | 2010 | 20308376 |
| maggot excretions inhibit biofilm formation on biomaterials. | biofilm-associated infections in trauma surgery are difficult to treat with conventional therapies. therefore, it is important to develop new treatment modalities. maggots in captured bags, which are permeable for larval excretions/secretions, aid in healing severe, infected wounds, suspect for biofilm formation. therefore we presumed maggot excretions/secretions would reduce biofilm formation. | 2010 | 20309656 |
| potential role of active surveillance in the control of a hospital-wide outbreak of carbapenem-resistant klebsiella pneumoniae infection. | the recent emergence of carbapenem resistance among enterobacteriaceae is a major threat for hospitalized patients, and effective strategies are needed. | 2010 | 20370465 |
| studies on amino acid replacement and inhibitory activity of a beta-lactamase inhibitory peptide. | an shv beta-lactamase gene was amplified from a beta-lactam resistant klebsiella pneumoniae k-71 genomic dna. after expression and purification, we demonstrated that peptide p1 could inhibit the hydrolysis activity of both tem-1 and shv beta-lactamase in vitro. three mutations were introduced into p1 in which the first residue s was replaced by f, the 18th residue v was mutated to y, and the 15th residue y was substituted with a, c, g, and r to obtain the mutants of p1-a, p1-c, p1-g, and p1-r, r ... | 2010 | 20370612 |
| the global problem of antibiotic resistance. | amid the recent attention justly focused on the potential problem of microbial sources for weapons of bioterrorism, it is also apparent that human pathogens frequently isolated from infections in patients from community and hospital sources have been growing more resistant to commonly used antibiotics. much of the growth of multiple-drug-resistant (mdr) bacterial pathogens can be contributed to the overuse of broad-spectrum antimicrobial products. however, an equally troubling and often overlook ... | 2010 | 20370622 |
| carbapenem-resistant klebsiella pneumoniae bacteremia: factors correlated with clinical and microbiologic outcomes. | we undertook a retrospective cohort study describing general outcomes and specific factors associated with positive outcomes in bacteremia due to carbapenem-resistant klebsiella pneumoniae (crkp). forty-eight patients were included, of which 42% died at 30 days. forty-two percent of patients were in septic shock at the time of the first positive blood culture, and 42% were recipients of solid organ transplants. lack of microbiologic eradication at 7 days was independently associated with 30-day ... | 2010 | 20356699 |
| plasmid-mediated carbapenem-hydrolysing oxa-48 beta-lactamase in klebsiella pneumoniae from tunisia. | 2010 | 20356714 | |
| characterisation of qnr plasmid-mediated quinolone resistance in enterobacteriaceae from italy: association of the qnrb19 allele with the integron element iscr1 in escherichia coli. | the spread of plasmid-mediated quinolone resistance determinants (qnr-like determinants) was evaluated in a collection of 232 ciprofloxacin-resistant or extended-spectrum beta-lactamase (esbl)-producing enterobacterial isolates recovered between november 2007 and may 2008 at padua university hospital, italy. qnr genes were mainly found in klebsiella pneumoniae (68%) and to a lesser extent in escherichia coli (5.1%). among the qnra1, qnrs1 and qnrb19 alleles found, the latter was by far the most ... | 2010 | 20356715 |
| molecular architecture of the mn2+-dependent lactonase ulag reveals an rnase-like metallo-beta-lactamase fold and a novel quaternary structure. | the ulag gene, located in the ula regulon, is crucial for the catabolism of l-ascorbate under anaerobic conditions and it has been proposed to encode for the putative l-ascorbate-6-p lactonase. the ulag gene is widespread among eubacteria, including human commensal and pathogenic genera such as escherichia, shigella, klebsiella and salmonella. here, we report the three-dimensional structures of the apoenzyme and mn(2+) holoenzyme of ulag from e. coli to 2.6 a resolution, determined using single- ... | 2010 | 20359483 |
| hospital outbreak control requires joint efforts from hospital management, microbiology and infection control. | an outbreak of multidrug-resistant klebsiella pneumoniae producing the extended-spectrum beta-lactamase ctx-m15 affected 247 mainly elderly patients in more than 30 wards in a 1000-bedded swedish teaching hospital between may 2005 and august 2007. a manual search of the hospital administrative records for possible contacts between cases in wards and outpatient settings revealed a complex chain of transmission. faecal screening identified twice as many cases as cultures from clinical samples. tra ... | 2010 | 20359768 |
| distinct bacterial colonization patterns of escherichia coli subtypes associate with rheumatoid factor status in early inflammatory arthritis. | the aetiology of ra is unknown; however, bacterial exposure, particularly to escherichia coli, proteus mirabilis and klebsiella pneumoniae, has been linked to disease pathogenesis. the strongest association was observed for rf(+) ra. we compare colonization patterns of these bacteria, and the anti-bacterial antibody levels in early onset rf(+) and rf(-) inflammatory arthritis. | 2010 | 20360042 |
| predominance of an st11 extended-spectrum beta-lactamase-producing klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in korea. | to investigate the antimicrobial resistance, extended-spectrum beta-lactamases (esbls) and clones of klebsiella pneumoniae isolates causing bacteraemia or urinary tract infection (uti) in korea, a total of 406 k. pneumoniae isolates from patients with bacteraemia (221 isolates) and uti (185 isolates) were collected from 10 tertiary-care korean hospitals from july 2006 to october 2007. in vitro antimicrobial susceptibility testing was performed for all isolates and esbl production was tested. mul ... | 2010 | 20360396 |
| cooperative interactions between tlr4 and tlr9 regulate interleukin 23 and 17 production in a murine model of gram negative bacterial pneumonia. | toll like receptors play an important role in lung host defense against bacterial pathogens. in this study, we investigated independent and cooperative functions of tlr4 and tlr9 in microbial clearance and systemic dissemination during gram-negative bacterial pneumonia. to access these responses, wildtype balb/c mice, mice with defective tlr4 signaling (tlr4(lps-d)), mice deficient in tlr9 (tlr9(-/-)) and tlr4/9 double mutant mice (tlr4(lps-d)/tlr9(-/-)) were challenged with k. pneumoniae, then ... | 2010 | 20360853 |
| enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture. | in fed-batch culture of klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. when the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. high concentrations of pdo and acetate ... | 2010 | 20361229 |
| enhanced production of 2,3-butanediol from glycerol by forced ph fluctuations. | the glycerol fermentation by klebsiella pneumoniae occurs by receiving more than five liquid products-organic acids, diols, and ethanol. aiming to direct the glycerol conversion towards predominant production of 2,3-butanediol (2,3-bd), the main influencing parameters (the aeration and the ph) were investigated during fed-batch processes. the regime of intensive aeration (2.2 vvm air supply) was evaluated as most favorable for 2,3-bd synthesis and ensured the decrease of all other metabolites. t ... | 2010 | 20361325 |
| identification and characterization of th cell epitopes in mrkd adhesin of klebsiella pneumoniae. | in this study, we identified the th epitopes in mrkd of klebsiella pneumoniae, an excellent vaccine candidate antigen. by using the rankpep prediction algorithm, we have identified and characterized three th epitopes within the mrkd antigen, which can be recognized by cd4+ t cells from balb/c (h-2(d)) mice. they were m(221-235), m(175-189), and m(264-278). these epitopes have important value for studying the immune response of k. pneumoniae infection and for designing effective vaccine against k ... | 2010 | 20362045 |
| prospects for new antimicrobials based on n,n-dimethylbiguanide complexes as effective agents on both planktonic and adhered microbial strains. | metal-free n,n-dimethylbiguanidium acetate and novel complexes m(dmbg)(2)(ch(3)coo)(2).nh(2)o (m: mn(ii), ni(ii), cu(ii) and zn(ii)) were screened for their antimicrobial properties against gram-positive (bacillus subtilis, listeria monocytogenes, staphylococcus aureus), gram-negative (klebsiella pneumoniae, escherichia coli, pseudomonas aeruginosa) bacteria and fungal (candida albicans) strains. the ability of compounds to inhibit the microbial adherence ability to the inert substratum as well ... | 2010 | 20363535 |
| anterior sacral pyocele with meningitis: a rare presentation of occult spinal dysraphism with congenital dermal sinus. | the authors describe an interesting case of a hitherto asymptomatic occult spinal defect with a congenital sacral dermal sinus which proved to be the entry point for bacterial meningitis in an otherwise healthy 9-year-old female child. the patient presented with fever and neck stiffness, and a dermal sinus in the lumbosacral region was identified on examination. cerebrospinal fluid analysis confirmed bacterial meningitis and a spinal magnetic resonance imaging scan revealed a dermal sinus tract ... | 2010 | 20363962 |
| in vitro double and triple bactericidal activities of doripenem, polymyxin b, and rifampin against multidrug-resistant acinetobacter baumannii, pseudomonas aeruginosa, klebsiella pneumoniae, and escherichia coli. | in vitro double and triple bactericidal activities of doripenem, polymyxin b, and rifampin were assessed against 20 carbapenem-resistant clinical isolates with different mechanisms of carbapenem resistance. bactericidal activity was achieved in 90% of all bacteria assayed using combinations of polymyxin b, doripenem, and rifampin against five each of the carbapenem-resistant klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and escherichia coli isolates studied. combination ... | 2010 | 20368401 |
| carbapenem resistance via the blakpc-2 gene in enterobacter cloacae blood culture isolate. | an enterobacter cloacae blood culture isolate expressing carbapenem resistance via the klebsiella pneumoniae carbapenemase kpc-2 gene is reported. to our knowledge, this is the first report of a nosocomial isolate with carbapenemase-mediated resistance causing infection in a patient from tennessee. | 2010 | 20375954 |
| production of 2,3-butanediol from corncob molasses, a waste by-product in xylitol production. | corncob molasses, a waste by-product in xylitol production, contains high concentrations of mixed sugars. in the present study, corncob molasses was used to produce 2,3-butanediol (bd) using klebsiella pneumoniae sdm. this was the first report on the use of corncob molasses to produce bulk chemicals. our results indicated that k. pneumoniae sdm can utilize various sugars contained in the corncob molasses in a preferential manner: glucose > arabinose > xylose. it was shown that high sugars concen ... | 2010 | 20376634 |
| recurrent gram-negative bloodstream infection: a 10-year population-based cohort study. | recurrent gram-negative bloodstream infection (bsi) has not been evaluated in a population-based setting; therefore, we performed a population-based retrospective cohort study to examine the incidence, recurrence, and mortality rates of gram-negative bsi. | 2010 | 20378069 |
| osteopontin promotes host defense during klebsiella pneumoniae-induced pneumonia. | klebsiella pneumoniae is a common cause of nosocomial pneumonia. osteopontin (opn) is a phosphorylated glycoprotein involved in inflammatory processes, some of which is mediated by cd44. the aim of this study was to determine the role of opn during k. pneumoniae-induced pneumonia. wild-type (wt) and opn knockout (ko) mice were intranasally infected with 10⁴ colony forming units of k. pneumoniae, or administered klebsiella lipopolysaccharides (lps). in addition, recombinant opn (ropn) was intrana ... | 2010 | 20378602 |
| regulation of the klebsiella pneumoniae kpc fimbriae by the site-specific recombinase kpci. | in the genome of klebsiella pneumoniae ntuh-k2044, nine fimbrial gene clusters were identified. besides type 1 and type 3 fimbriae, the others are novel and were named kpa, kpb, kpc, kpd, kpe, kpf and kpg fimbriae. prevalence analysis among 105 k. pneumoniae clinical isolates revealed that the kpc genes were highly associated with the k1 serotype isolates. induced expression of the recombinant kpcabcd genes in escherichia coli resulted in kpc fimbriation and increased biofilm formation. a putati ... | 2010 | 20378654 |
| detection and treatment options for klebsiella pneumoniae carbapenemases (kpcs): an emerging cause of multidrug-resistant infection. | bacteria producing klebsiella pneumoniae carbapenemases (kpcs) are rapidly emerging as a cause of multidrug-resistant infections worldwide. bacterial isolates harbouring these enzymes are capable of hydrolysing a broad spectrum of beta-lactams including the penicillins, cephalosporins, carbapenems and monobactam. detection of isolates harbouring carbapenemases can be inconsistent using automated systems, often requiring subsequent confirmatory tests. phenotypic methods utilizing boronic acid dis ... | 2010 | 20378670 |
| inactivation of dhad and dhak abolishes by-product accumulation during 1,3-propanediol production in klebsiella pneumoniae. | 1,3-propanediol (1,3-pd) can be used for the industrial synthesis of a variety of compounds, including polyesters, polyethers, and polyurethanes. 1,3-pd is generated from petrochemical and microbial sources. 1,3-propanediol is a typical product of glycerol fermentation, while acetate, lactate, 2,3-butanediol, and ethanol also accumulate during the process. substrate and product inhibition limit the final concentration of 1,3-propanediol in the fermentation broth. it is impossible to increase the ... | 2010 | 20379761 |
| endoscopy-associated transmission of carbapenem-resistant klebsiella pneumoniae producing kpc-2 beta-lactamase. | 2010 | 20382724 | |
| rmpa regulation of capsular polysaccharide biosynthesis in klebsiella pneumoniae cg43. | sequence analysis of the large virulence plasmid plvpk in klebsiella pneumoniae cg43 revealed the presence of another mucoid factor encoding gene rmpa besides rmpa2. promoter activity measurement indicated that the deletion of rmpa reduced k2 capsular polysaccharide (cps) biosynthesis, resulting in decreased colony mucoidy and virulence in mice. introduction of a multicopy plasmid carrying rmpa restored cps production in the rmpa or rmpa2 mutant but not in the rcsb mutant. transformation of the ... | 2010 | 20382770 |
| correlation between klebsiella pneumoniae carrying plvpk-derived loci and abscess formation. | klebsiella pneumoniae-caused liver abscess (kla) is an emerging infectious disease. however, factors other than k1-specific loci that contribute to the pathogenesis of this disease have not been identified. plvpk is a 219,385-bp plasmid of k. pneumoniae cg43, an invasive k2 strain associated with kla. we aimed in this study to evaluate the involvement of plvpk in k. pneumoniae virulence and its clinical significance in abscess formation. a plvpk-cured cg43 was isolated and its virulence was exam ... | 2010 | 20383552 |
| antibacterial activity of alpinia galanga (l) willd crude extracts. | methanol, acetone and diethyl ether extracts of alpinia galanga have been evaluated against pathogens viz. bacillus subtilis mtcc 2391, enterobacter aerogene, enterobacter cloacae, enterococcus faecalis, escherichia coli mtcc 1563, klebsiella pneumoniae, pseudomonas aeruginosa mtcc 6642, salmonella typhimurium, staphylococcus aureus and streptococcus epidermis using agar well diffusion method. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of all the extracts ... | 2010 | 20387130 |
| emergence of ctx-m group 1 extended-spectrum beta-lactamase-producing klebsiella pneumoniae strains in the community. | molecular characterization of esbl-producing k. pneumoniae strains isolated from urine of outpatients in zagreb region during the last five years. | 2010 | 20387722 |
| [characterization of esbl-producing escherichia coli and klebsiella pneumoniae strains isolated from urine of nonhospitalized patients in the zagreb region]. | to determine the prevalence of esbl-producing escherichia coli and klebsiella pneumoniae strains isolated from urine of nonhospitalized patients during a three-year period, to determine their antibiotic susceptibility, investigate the transfer of esbl genes with cotransfer of resistance and to characterize isolated beta-lactamases. | 2010 | 20387724 |
| etiology and outcome of community-acquired lung abscess. | anaerobes are the first and streptococcus species the second most common cause of community-acquired lung abscess (cala) in the west. the etiologic pathogens of this disease have changed in taiwan, with klebsiella pneumoniae being reported as the most common cause of cala. | 2010 | 20389050 |
| expanded role for the nitrogen assimilation control protein in the response of klebsiella pneumoniae to nitrogen stress. | klebsiella pneumoniae is able to utilize many nitrogen sources, and the utilization of some of these nitrogen sources is dependent on the nitrogen assimilation control (nac) protein. seven nac-regulated promoters have been characterized in k. pneumoniae, and nine nac-regulated promoters have been found by microarray analysis in escherichia coli. so far, all characterized nac-regulated promoters have been directly related to nitrogen metabolism. we have used a genome-wide analysis of nac binding ... | 2010 | 20348267 |
| [microbiological etiology in children with community acquired pneumonia]. | to study the distribution of pathogenic microorganisms of community acquired pneumonia (cap) in children. | 2010 | 20350426 |
| [effects of probiotics on intestinal bacterial colonization in premature infants]. | to study the possible roles of probiotics in decreasing intestinal bacterial colonization rate and the incidence of enterogenic infections in premature infants. | 2010 | 20350428 |
| investigation of extended-spectrum beta-lactamase-producing klebsiella pneumoniae outbreaks in hungary between 2005 and 2008. | fourteen outbreaks in hungary between 2005 and 2008 caused by extended-spectrum beta-lactamase-producing klebsiella pneumoniae (esbl-kp) were epidemiologically investigated and the isolated pathogens were characterized by molecular techniques. ten of the fourteen outbreaks occurred in adult wards and four in neonatal units affecting a total number of 73 patients. the 54% [40] of the patients developed bloodstream infections and 21.9%-21.9% [16] pneumonia and surgical site infections, respectivel ... | 2010 | 20350878 |
| ramr mutations in clinical isolates of klebsiella pneumoniae with reduced susceptibility to tigecycline. | five klebsiella pneumoniae isolates with reduced susceptibility to tigecycline (mic, 2 microg/ml) were analyzed. a gene homologous to ramr of salmonella enterica was identified in klebsiella pneumoniae. sequencing of ramr in the nonsusceptible klebsiella strains revealed deletions, insertions, and point mutations. transformation of mutants with wild-type ramr genes, but not with mutant ramr genes, restored susceptibility to tigecycline and repressed overexpression of rama and acrb. thus, this st ... | 2010 | 20350947 |
| molecular epidemiology, sequence types, and plasmid analyses of kpc-producing klebsiella pneumoniae strains in israel. | sporadic isolates of carbapenem-resistant kpc-2-producing klebsiella pneumoniae were isolated in tel aviv medical center during 2005 and 2006, parallel to the emergence of the kpc-3-producing k. pneumoniae sequence type 258 (st 258). we aimed to study the molecular epidemiology of these isolates and to characterize their bla(kpc)-carrying plasmids and their origin. ten isolates (8 kpc-2 and 2 kpc-3 producing) were studied. all isolates were extremely drug resistant. they possessed the bla(kpc) g ... | 2010 | 20350950 |
| diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant. | the occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, beta-lactam/beta-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. additionally the removal of resistant isolates was evaluated in the laboratory-scale wastewater treatment model plant (m-wwtp), continuously suppli ... | 2010 | 20351416 |
| antibiotic resistance in mexico: a brief overview of the current status and its causes. | as in many other developing countries, conditions that may foster antibiotic resistance in mexico differ from developed countries, and so resistance prevalence. fecal pollution and other traits of overcrowded, poor cities, might create ideal settings for selecting, exchanging and maintaining resistance traits. medical abuse of antibiotics, along with low-quality drugs, are also present as in many developing countries. self-prescription, a common yet unmeasured practice among mexican population, ... | 2010 | 20351451 |
| high prevalence of extended-spectrum beta-lactamase-producing klebsiella pneumoniae in a tertiary care hospital in tehran, iran. | extended-spectrum beta (beta)-lactamase (esbl)-producing enterobacteria are major emerging pathogens in nosocomial infections. | 2010 | 20351452 |
| enhanced production of ethanol from glycerol by engineered hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from zymomonas mobilis. | to improve production of ethanol from glycerol, the methylotrophic yeast hansenula polymorpha was engineered to express the pdc and adhb genes encoding pyruvate decarboxylase and aldehyde dehydrogenase ii from zymomonas mobilis, respectively, under the control of the glyceraldehyde-3-phosphate dehydrogenase (gapdh) promoter. the ethanol yield was 3.3-fold higher (2.74 g l(-1)) in the engineered yeast compared with the parent strain (0.83 g l(-1)). further engineering to stimulate glycerol utiliz ... | 2010 | 20354759 |
| essential oil from the underground parts of laserpitium zernyi: potential source of alpha-bisabolol and its antimicrobial activity. | the composition and antimicrobial activity of the essential oil from the underground parts (roots and rhizomes) of laserpitium zernyi hayek (apiaceae) were investigated. the pale blue essential oil, obtained by hydrodistillation, was analyzed by gc and gc-ms. forty-three compounds were identified (94.3% of total oil). the main constituent, beside alpha-pinene (31.6%) was alpha-bisabolol (30.9%), so this oil may be a novel potential natural source of this sesquiterpene alcohol. the antimicrobial ... | 2010 | 20334149 |
| multidrug resistance among gram-negative pathogens that caused healthcare-associated infections reported to the national healthcare safety network, 2006-2008. | we evaluated isolates of klebsiella pneumoniae, pseudomonas aeruginosa, and acinetobacter baumannii that were reported to the national healthcare safety network from january 2006 through december 2008 to determine the proportion that represented multidrug-resistant phenotypes. the pooled mean percentage of resistance varied by the definition used; however, multidrug resistance was relatively common and widespread. | 2010 | 20334552 |
| outbreak of carbapenem-resistant klebsiella pneumoniae in puerto rico associated with a novel carbapenemase variant. | carbapenem-resistant klebsiella pneumoniae (crkp) is resistant to almost all antimicrobial agents, and crkp infections are associated with substantial morbidity and mortality. | 2010 | 20334553 |
| [diversity changes of the microbial communities and bla(ctx-m) genes in urban river sediments treated with cefotaxime]. | to explore the diversity changes of microbial communities and bla(ctx-m) in an urban river sediment community with cefotaxime treatment. | 2010 | 20335110 |
| isolation of some pathogenic bacteria from the great spruce bark beetle, dendroctonus micans and its specific predator, rhizophagus grandis. | some bacteria were isolated from dendroctonus micans and its specific predator, rhizophagus grandis. six bacteria from d. micans were identified as bacillus pumilus, enterobacter intermedius, citrobacter freundii, cellulomonas flavigena, microbacterium liquefaciens and enterobacter amnigenus, three bacteria from r. grandis as klebsiella pneumoniae, pantoea agglomerans and serratia grimesii, on the basis of fatty acid methyl ester analysis and carbon utilization profile by using microbial identif ... | 2010 | 20336502 |
| temperature and ph affect the production of bacterial biofilm. | the effect of different cultivation temperatures (30 and 37 degrees c) and ph of the media (5.5, 7.5, 8.5) on the biofilm production was compared in pseudomonas aeruginosa, klebsiella pneumoniae, and vibrio cholerae non-o1 and o1 using the crystal-violet test for estimation of quantitative production of the biofilm. decrease (46.4-98.4 %) in the biofilm production was observed at 37 degrees c in 8 of the tested strains (p. aeruginosa three strains, k pneumoniae two, v. cholerae non-o1 two, and v ... | 2010 | 20336508 |
| severe mediastinal infection with abscess formation after endobronchial ultrasound-guided transbrochial needle aspiration. | endobronchial, ultrasound-guided, transbronchial needle aspiration has recently been introduced as an alternative to mediastinoscopy for lymph node staging of lung cancer and the diagnosis of respiratory diseases. this procedure is less invasive and more cost-effective, and multiple large studies have reported no associated complications. in this case, an individual presented with descending mediastinitis after having this minimally invasive procedure for mediastinal lymphadenopathy. | 2010 | 20338352 |
| impact of hospital-wide infection rate, invasive procedures use and antimicrobial consumption on bacterial resistance inside an intensive care unit. | we performed a 30-month ecological study to determine the impact of hospital-wide antibiotic consumption, invasive procedure use and hospital-acquired infections (hais) on antibiotic resistance in an intensive care unit (icu). microbiological isolates from icu patients with established diagnosis of hospital infection were monitored throughout the study. overall hospital consumption per 100 patient-days of piperacillin-tazobactam, fluoroquinolones and cephalosporins increased from 1.9 to 2.3 defi ... | 2010 | 20338669 |
| imipenem-associated encephalopathy: alert to physicians. | 2010 | 20339671 | |
| characterization of the nasal, sublingual, and oropharyngeal mucosa microbiota in cleft lip and palate individuals before and after surgical repair. | to delineate inherent differences in the microbial milieu in cleft palate patients compared with cleft lip patients and to document changes in microbial flora before and after cleft lip and palate repair. | 2010 | 20210635 |
| contribution of outer membrane protein k36 to antimicrobial resistance and virulence in klebsiella pneumoniae. | loss of outer membrane protein (omp) is commonly encountered in multidrug-resistant klebsiella pneumoniae. however, little is known about the association between omp loss and virulence. in the present study, this association was investigated in k. pneumoniae. | 2010 | 20211860 |
| treatment with fluoroquinolones or with beta-lactam-beta-lactamase inhibitor combinations is a risk factor for isolation of extended-spectrum-beta-lactamase-producing klebsiella species in hospitalized patients. | antibiotic exposure exerts strong selective pressure and is an important modifiable risk factor for antibiotic resistance. we aimed to identify the role of various antibiotics as risk factors for the isolation of extended-spectrum-beta-lactamase (esbl)-producing klebsiella spp. in hospitalized patients at a tertiary-care hospital. a parallel multivariable model was created to compare two groups of cases with either nosocomially acquired esbl- or non-esbl-producing klebsiella spp. to a common con ... | 2010 | 20211888 |
| worldwide experience with the use of doripenem against extended-spectrum-beta-lactamase-producing and ciprofloxacin-resistant enterobacteriaceae: analysis of six phase 3 clinical studies. | the worldwide increase in fluoroquinolone-resistant and extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae pathogens has led to doripenem and other carbapenems assuming a greater role in the treatment of serious infections. we analyzed data from 6 phase 3 multinational doripenem clinical trials on ciprofloxacin-resistant enterobacteriaceae isolates consisting of all genera (cipre) and esbl-producing enterobacteriaceae isolates consisting of escherichia coli, klebsiella spp., an ... | 2010 | 20211892 |
| kpc-producing extreme drug-resistant klebsiella pneumoniae isolate from a patient with diabetes mellitus and chronic renal failure on hemodialysis in south korea. | 2010 | 20211897 | |
| antimicrobial resistance in pathogens causing nosocomial infections in surgery and intensive care units of two hospitals in antananarivo, madagascar. | in developing countries, knowledge of antimicrobial resistance patterns is essential to define empirical therapy. | 2010 | 20212337 |
| first outbreak of klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae in germany. | we report the first outbreak of klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae in germany. the presence of kpc was confirmed by polymerase chain reaction (pcr). the kpc-encoding plasmid was analysed by transconjugation experiments, dna sequencing, southern blotting and isoelectric focussing. typing was performed by pulsed-field gel electrophoresis (pfge). an ertapenem-resistant k. pneumoniae with low minimum inhibitory concentrations (mic) to other cabapenems (tested by the vi ... | 2010 | 20213255 |
| antibiotic resistance trends in paediatric community-acquired first urinary tract infections in the united arab emirates. | we analysed the trend of antibiotic resistance of community-acquired uropathogens over a 4-year period in a cohort of children with a first episode of culture-proven urinary tract infection presenting to the department of paediatrics at a large general secondary care hospital in the united arab emirates. comparisons were made between 2 time periods: 2003-04 versus 2005-06 for 145 children aged 2 months to 12 years of age. although some changes in resistance were observed for some antibiotics bet ... | 2010 | 20214157 |
| photoantimicrobials as a potential local approach to geriatric utis. | to test the efficacy of acceptable photoantimicrobial agents against bacterial pathogens implicated in complicated urinary tract infection (uti) in comparison with conventionally employed antibacterials. | 2010 | 20214732 |
| isolation of multidrug-resistant klebsiella oxytoca carrying blaimp-8, associated with oxy hyperproduction, in the intensive care unit of a community hospital in spain. | 2010 | 20215129 | |
| clinical characteristics of nosocomial infections in neonatal intensive care unit in eastern china. | a retrospective cohort study on nosocomial infections (ni) in the neonatal intensive care unit (nicu) was performed in the children's hospital of zhejiang university, hangzhou district, china. the most common infection site was pneumonia and bloodstream infection. low admission age, long nicu stay, and mechanical ventilation were significant risk factors for ni. klebsiella pneumoniae was the most common pathogen, followed by acinetobacter baumannii, staphylococcus epidermidi, pseudomonas aerugin ... | 2010 | 20297900 |
| utilization of amino acids by bacteria from the pig small intestine. | this study determined the utilization of amino acids (aa) by bacteria from the lumen of the pig small intestine. digesta samples from different segments of the small intestine were inoculated into media containing 10 mmol/l each of select aa (l-lysine, l-threonine, l-arginine, l-glutamate, l-histidine, l-leucine, l-isoleucine, l-valine, l-proline, l-methionine, l-phenylalanine or l-tryptophan) and incubated for 24 h. the previous 24-h culture served as an inoculum for a subsequent 24-h subcultur ... | 2010 | 20300787 |
| septic shock due to klebsiella pneumoniae after medical abortion with misoprostol-only regimen. | to report a case of a healthy woman who was admitted to the hospital with septic shock caused by a common uropathogen after self-administration of misoprostol for pregnancy termination. | 2010 | 20303487 |
| substrate specificity and evolutionary implications of a nifdk enzyme carrying nifb-co at its active site. | the in vitro reconstitution of molybdenum nitrogenase was manipulated to generate a chimeric enzyme in which the active site iron-molybdenum cofactor (femo-co) is replaced by nifb-co. the nifdk/nifb-co enzyme was unable to reduce n(2) to nh(3), while exhibiting residual c(2)h(4) and considerable h(2) production activities. production of h(2) by nifdk/nifb-co was stimulated by n(2) and was dependent on nifh and atp hydrolysis. thus, nifdk/nifb-co is a useful tool to gain insights into the catalyt ... | 2010 | 20219465 |
| [age-related features of dysbiosis of large intestine]. | to compare rate of detection of stage ii-iii intestinal dysbiosis with normal and decreased quantities of bacteria from lactobacillus and bifidobacterium families in different age groups. | 2010 | 20222187 |