Publications

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degradation of crude oil by acinetobacter calcoaceticus and alcaligenes odorans.two types of indian crude oil (bombay high and gujarat) were tested for their biodegradability by acinetobacter calcoaceticus and alcaligenes odorans. acinetobacter calcoaceticus s30 and alc. odorans p20 degraded bombay high crude oil by 50% and 45%, while only 29% and 37% of gujarat crude oil (heavy crude oil) was degraded by these isolates, respectively. acinetobacter calcoaceticus and alc. odorans in combination degraded 58% and 40% of bombay high and gujarat crude oils, respectively, which w ...19968896350
multiparametric analysis of waterline contamination in dental units.microbial contamination of dental unit waterlines is thought to be the result of biofilm formation within the small-bore tubing used for these conduits. systematic sampling of 121 dental units located at the dental school of université de montréal showed that none of the waterlines was spared from bacterial contamination. multilevel statistical analyses showed significant differences between samples taken at the beginning of the day and samples taken after a 2-min purge. differences were also fo ...19968899982
the beta-ketoadipate pathway and the biology of self-identity.the beta-ketoadipate pathway is a chromosomally encoded convergent pathway for aromatic compound degradation that is widely distributed in soil bacteria and fungi. one branch converts protocatechuate, derived from phenolic compounds including p-cresol, 4-hydroxybenzoate and numerous lignin monomers, to beta-ketoadipate. the other branch converts catechol, generated from various aromatic hydrocarbons, amino aromatics, and lignin monomers, also to beta-ketoadipate. two additional steps accomplish ...19968905091
a comparative study of different pcr-based dna fingerprinting techniques for typing of the acinetobacter calcoaceticus-a. baumannii complex.different pcr-based dna fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the acinetobacter calcoaceticus-a. baumannii complex. seven isolates belonged to a previously defined outbreak while 19 isolates were unrelated epidemiologically. the pcr-based dna fingerprinting techniques used were: (i) repetitive extragenic palindromic (rep) pcr; (ii) enterobacterial repetitive intergenic consensus (eric) pcr; (iii) randomly amplified polymorphic dna with m13 forward ...19968636966
[identification and typing of hospital strains of acinetobacter calcoaceticus-acinetobacter baumanni complex].a collection of 95 strains of the acinetobacter calcoaceticus-acinetobacter baumannii complex, isolated between 1991 and 1993 in the prague burn center (bc), was studied. ninety-one strains were isolated from 43 patients: 50 of them from burnt sites, 22 from endotracheal tube, 13 from urine, 3 from blood and 3 from venous catheter, and 4 strains were isolated from the hospital environment and the nursing staff. the strains were classified by restriction endonuclease fingerprinting of total dna, ...19968756404
is1236, a newly discovered member of the is3 family, exhibits varied patterns of insertion into the acinetobacter calcoaceticus chromosome.analysis of spontaneous mutations in acinetobacter calcoaceticus revealed a 1237 bp insertion sequence named is1236 and possessing a nucleotide sequence resembling those of members of the ls3 family. the chromosome of a. calcoaceticus strain adp1 contains seven copies of is1236 which appears to insert preferentially into pobr, the transcriptional activator of the structural gene for p-hydroxybenzoate hydroxylase. is1236 creates tandem 3 bp dna duplications flanking the sites of its insertion in ...19968757745
a reappraisal of the diversity and class distribution of aspartate transcarbamoylases in gram-negative bacteria.recently, the subunit composition of class a aspartate transcarbamoylases (atcases) in fluorescent pseudomonads has been clarified. we present evidence that distribution of this type of atcase may be more widespread than at first suspected. bacterial atcases exist in three forms: class a (molecular mass approximately 450-500 kda); class b, typified by escherichia coli atcase (approximately 300 kda); and class c, typified by bacillus subtilis atcase (approximately 100 kda). using gradient gel ele ...19968757751
evaluation of the ability of a commercial system to identify acinetobacter genomic species.a collection of 130 acinetobacter strains identified by dna hybridization to 18 different genomic species was used to assess the ability of the api 20ne system (biomérieux, france) to identify acinetobacter genomic species and to determine its accuracy. fifty-eight (87%) of the 67 strains of genomic species defined in the database (version 5.1) were identified to the appropriate genomic species. the acinetobacter baumannii strains and the acinetobacter haemolyticus strains were all identified co ...19968781881
[in vitro antibacterial activity of a new parenteral penem, sulopenem].eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ...19968786624
[in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic].the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ...19968786626
changing trends in the antimicrobial susceptibility of clinical isolates of acinetobacter baumannii.acinetobacter baumannii has recently emerged as an important nosocomial pathogen because of its resistance to the majority of commonly used antimicrobial agents. therefore, the pattern of isolation of acinetobacter baumannii in our laboratory and the in vitro susceptibilities to various antimicrobial agents were studied. one hundred and two clinical isolates of previously identified acinetobacter calcoaceticus var. anitratus from different patients were collected from the diagnostic bacteriology ...19968799001
in-vitro interaction of azithromycin and fluoroquinolones against gram-positive and gram-negative bacteria.objective: to determine the combined in-vitro effects of azithromycin plus the fluoroquinolone ofloxacin or lomefloxacin against gram-positive and gram-negative bacteria. methods: fractional inhibitory (fic) and fractional bactericidal concentration indices of azithromycin and the fluoroquinolone were determined using a microtiter-checkerboard method. clinical isolates of staphylococcus aureus, streptococcus pneumoniae, neisseria gonorrhoeae, escherichia coli, klebsiella pneumoniae, pseudomonas ...199611866755
polymicrobial bacteremia in cancer patients: analysis of risk factors, etiology and outcome in 214 episodes.two hundred and fourteen episodes of polymicrobial bacteremia in 182 cancer patients in a period of 6 years in a 360-bed national cancer institute were analyzed for etiology, risk factors and outcome. variables were compared with 187 episodes of monomicrobial bacteremias in 147 cancer patients to find statistical significance among risk factors, etiology and outcome. urinary catheters and breakthrough bacteremia were the only risk factors associated with polymicrobial in comparison to monomicrob ...199618611744
classification, error detection, and reconciliation of process information in complex biochemical systems.bioprocess identification starts with collection of process information. usually there is a variety of information available, consisting of actual measurement data, historical data, empirical kinetic and yield correlations, and general knowledge available from literature. a central problem is to find out how the various pieces of information should be integrated. in addition, one should know how to deal with missing, inconsistent, or too inaccurate data. recently, a general systematic method for ...199618623590
ptim3, a plasmid delivery vector for a transposon-based inducible marker gene system in gram-negative bacteria.ptim3 is a suicide plasmid vector for the delivery of a transposition defective derivative of tn5, expressing inducible mercury resistance (hgr) and catechol 2,3-dioxygenase (c23o) activity, to a range of gram-negative microorganisms. ptim3 was constructed by a four-stage process from pnmm1, a derivative of psup5011 containing a modified tn5 where antibiotic-resistance determinants have been replaced by the mer operon of tn501 and tdnc (encoding a c23o). in ptim3, tdnc is fused to merd, to bring ...19958825369
antibiotic resistance of acinetobacter calcoaceticus strains isolated from patients treated in intensive care units.the distribution according to specimens and susceptibility to antimicrobial agents of 481 acinetobacter calcoaceticus strains isolated from patients treated in intensive care units were studied. they occurred most frequently in tracheal specimens and pus. using disk diffusion test the strains proved to be multiple resistant to ampicillin (86.3%), azlocillin (86.7%), mezlocillin (84.0%), cefamandole (99.7%), cefoxitin (94.1%), cefuroxime (90.6%), cefoperazone (84.9%), cefotaxime (82.0%), ceftriax ...19958689090
cloning and expression of dipeptidase from acinetobacter calcoaceticus atcc 23055.the gene encoding dipeptidase was cloned from acinetobacter calcoaceticus atc 23055. determination of the nucleotide sequence revealed that the gene had an open reading flame of 1,050 bp coding a protein of 350 amino acids. the deduced amino acid sequence showed 48.8% similarity to human renal dipeptidases and conserved two amino acid residues identified in human and pig renal dipeptidases as essential ones for the catalytic activity. purified recombinant enzyme expressed in escherichia coli did ...19958690717
antibacterial properties of am-1155, a new 8-methoxy quinolone.am-1155 is a new 8-methoxy quinolonecarboxylic acid with a broad spectrum of antibacterial activity. it inhibited more than 90% of clinical isolates of methicillin-susceptible staphylococcus aureus, streptococci, enterococcus faecalis, most of the enterobacteriaceae, acinetobacter calcoaceticus and haemophilus influenzae at the concentration of 0.39 mg/l. am-1155 was 2- to 16-fold more active than ciprofloxacin against gram-positive organisms including methicillin-resistant staphylococci. the an ...19958522459
tn5-directed cloning of pqq genes from pseudomonas fluorescens cha0: mutational inactivation of the genes results in overproduction of the antibiotic pyoluteorin.pseudomonas fluorescens cha0 produces several secondary metabolites, e.g., the antibiotics pyoluteorin (plt) and 2,4-diacetylphloroglucinol (phl), which are important for the suppression of root diseases caused by soil-borne fungal pathogens. a tn5 insertion mutant of strain cha0, cha625, does not produce phl, shows enhanced plt production on malt agar, and has lost part of the ability to suppress black root rot in tobacco plants and take-all in wheat. we used a rapid, two-step cloning-out proce ...19958526497
tolerance to acid in ph 5.0-grown organisms of potentially pathogenic gram-negative bacteria.a wide range of potentially pathogenic species of gram-negative bacteria were far more resistant to extreme acidity (ph 2.0-3.5) when cultured at ph 5.0 (habituated to acid) than after ph 7.0 culture. the differences were particularly great for citrobacter spp., enterobacter spp., klebsiella spp. and for vibrio parahaemolyticus; substantial habituation was also observed for proteus mirabilis and aeromonas formicans but the effect was less marked for serratia marcescens and acinetobacter calcoace ...19958554762
acinetobacter endocarditis in children: a case report and review of the literature.acinetobacter calcoaceticus, a gram-negative bacterium ubiquitous in soil, water and sewage, is a rare cause of endocarditis in children. the first case of acinetobacter endocarditis in an infant is described. this patient had underlying tetralogy of fallot with absent pulmonary valve. a review of the literature in english revealed only four other cases of acinetobacter endocarditis in children; three of whom had underlying congenital heart disease. like the other reported cases, this patient re ...19958557392
phenol degradation by acinetobacter calcoaceticus ncib 8250.acinetobacter calcoaceticus ncib 8250 utilizes phenol as sole source of carbon and energy via an ortho-cleavage pathway. the presence of ethanol in mixed substrate cultivations repressed the utilization of phenol. in fed batch cultivation the phenol tolerance was increased at least 2-fold. maximum degradation rates of 150 mg phenol/(1 h) and 280 mg phenol/(g h), respectively were observed. phenol hydroxylase is induced by its substrate and in parallel the catechol-1,2-dioxygenase is detectable. ...19958568644
differentiation of acinetobacter calcoaceticus sensu stricto from related acinetobacter species by electrophoretic polymorphism of malate dehydrogenase, glutamate dehydrogenase and catalase.acinetobacter baumannii, unnamed acinetobacter species 3 (studied by p.j.m. bouvet and p.a.d. grimont) and unnamed dna group 13 (studied by i. tjernberg and j. ursing) are the most prevalent acinetobacter species in hospitals. using the identification scheme of bouvet and grimont, it is sometimes difficult to differentiate these species from a. calcoaceticus sensu stricto, a species of the natural environment that has seldom been found associated with human infection. genetically identified acin ...19958584799
the reca protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of recas and 16s rrnas from the same species.the evolution of the reca protein was analyzed using molecular phylogenetic techniques. phylogenetic trees of all currently available complete reca proteins were inferred using multiple maximum parsimony and distance matrix methods. comparison and analysis of the trees reveal that the inferred relationships among these proteins are highly robust. the reca trees show consistent subdivisions corresponding to many of the major bacterial groups found in trees of other molecules including the alpha, ...19958587109
study of a hydrocarbon-utilizing and emulsifier-producing acinetobacter calcoaceticus strain isolated from heating oil.twenty bacterial strains were isolated from a sample of contaminated heating oil and screened for their ability to use petroleum and several common fuels as the sole source of carbon and energy. one of the isolates, named mm5, was able to grow on petroleum derivatives and brought about an emulsification of those compounds. gas chromatography studies showed that strain mm5 was able to degrade hydrocarbons of heating oil. mm5 has been tentatively identified as a strain of acinetobacter calcoacetic ...19958588840
infection of the pancreatic duct following pancreas transplantation with bladder drainage.combined kidney-pancreas transplantation represents a widely accepted therapy for endstage diabetic nephropathy. drainage of the pancreatic juice into the urinary bladder is the preferred surgical technique in most centers. between january 1987 and december 1994 a total of 85 pancreas transplantations with pancreatocystostomy were performed at the innsbruck university hospital. in all cases a polyvinylcatheter was placed into the pancreatic duct and drained transvesically to the exterior. during ...19958596128
genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in acinetobacter calcoaceticus ncib8250.we have mutated acinetobacter calcoaceticus ncib-8250 to growth deficiency on phenol as sole carbon source and isolated genes with similarity to phenol hydroxylase and catechol 1,2-dioxygenase by complementation. sequence analysis reveals the presence of six open reading frames (orfs) with similarities to a pseudomonas multicomponent phenol hydroxylase which are followed by an orf with similarity to cata from a. calcoaceticus adp1. transformation of these genes to adp1 confers the ability to gro ...19958596453
[the efficacy of sulbactam-ampicillin in a urinary infection due to acinetobacter calcoaceticus var. anitratus]. 19957475461
purification and characterization of acinetobacter calcoaceticus 4-hydroxybenzoate 3-hydroxylase after its overexpression in escherichia coli.4-hydroxybenzoate 3-hydroxylase [ec 1.14.13.2] from acinetobacter calcoaceticus was purified to homogeneity following the 40-fold overexpression of this gene (poba) in escherichia coli. overexpression was accomplished by placing the fola gene (encoding trimethoprim-resistant dihydrofolate reductase) directly downstream of the poba gene, and demanding growth of recombinants on elevated concentration of trimethoprim. presumably, the surviving variants have undergone a genetic alteration which allo ...19957490269
antibiotic resistance pattern and r-plasmids of acinetobacter calcoaceticus subsp. anitratus.during a 6 month period from march 1990 to august 1990, a total of 159 strains of acinetobacter calcoaceticus subsp. anitratus were isolated from various samples and studied for antibiotic resistance pattern to 12 drugs by kirby-bauer method. ceftazidime and netilmycin were the most sensitive drugs followed by cefotaxime, norfloxacin and augmentin. all the strains were resistant to chloramphenicol and tetracycline. commonest pattern of resistance was acgkstsu. forty eight isolates were tested fo ...19958919107
microbial colonization of large wounds.this study determines the nature of microbial wound colonization in 28 patients with large burns admitted to the burn centre, haukeland university hospital, bergen. altogether, 748 swabs were taken in 141 sampling procedures. a total of 414 microbial isolates were detected and their resistance patterns to a variety of systemic antimicrobial agents determined. the most frequent isolates were coagulase-negative staphylococci (21.5 per cent) and staphylococcus aureus (14 per cent), followed by ente ...19958747728
cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs.the two-component nonheme iron dioxygenase system 2-halobenzoate 1,2-dioxygenase from pseudomonas cepacia 2cbs catalyzes the double hydroxylation of 2-halobenzoates with concomitant release of halogenide and carbon dioxide, yielding catechol. the gene cluster encoding this enzyme, cbdabc, was localized on a 70-kbp conjugative plasmid designated pbah1. the nucleotide sequences of cbdabc and flanking regions were determined. in the deduced amino acid sequence of the large subunit of the terminal o ...19957530709
comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of acinetobacter isolates.seventy-three isolates of the acinetobacter calcoaceticus-acinetobacter baumannii complex, including 26 isolates from 10 hospital outbreaks, were typed by ribotyping with ecori and clai and by pulsed-field gel electrophoresis (pfge) of genomic dna after digestion with apai. ribotyping with ecori distinguished 31 ribopatterns. digestion with clai generated another eight ribotypes. pfge, in contrast, identified 49 distinct patterns with seven variants. both methods detected all outbreak-related is ...19957542274
the in-vitro activity of cp 99,219, a new naphthyridone antimicrobial agent: a comparison with fluoroquinolone agents.the in-vitro activity of a new fluoroquinolone, cp 99,219 was compared with that of ciprofloxacin, du 6859, sparfloxacin and levofloxacin. cp 99,219 showed generally similar in-vitro activity to the other compounds tested against the enterobacteriaceae (mic90 of all agents < 0.5 mg/l except morganella morganii). it was found to be more active than ciprofloxacin, sparfloxacin and levofloxacin against the strains of acinetobacter calcoaceticus tested (mic90 1 mg/l). cp 99,219 was found to be four- ...19957559198
biosensor based on an enzyme modified electrode for highly-sensitive measurement of polyphenols.the use of glucose dehydrogenase from acinetobacter calcoaceticus for highly sensitive measurement of polyphenols, based on bioelectrocatalytic analyte recycling, has been demonstrated. a polyphenol (analyte) is oxidized on the surface of a glassy carbon electrode at an anodic potential and is regenerated by immobilized glucose dehydrogenase (gdh) in the presence of glucose, resulting in an amplified response. the dynamic properties of the enzyme-modified glassy carbon electrode allow the conven ...19957576439
catm encodes a lysr-type transcriptional activator regulating catechol degradation in acinetobacter calcoaceticus.on the basis of the constitutive phenotypes of two catm mutants of acinetobacter calcoaceticus, the catm protein was proposed to repress expression of two different loci involved in catechol degradation, cata and catbcijfd (e. neidle, c. hartnett, and l. n. ornston, j. bacteriol. 171:5410-5421, 1989). in spite of its proposed negative role as a repressor, catm is similar in amino acid sequence to positive transcriptional activators of the lysr family. investigating this anomaly, we found that in ...19957592340
unusual ancestry of dehydratases associated with quinate catabolism in acinetobacter calcoaceticus.catabolism of quinate to protocatechuate requires the consecutive action of quinate dehydrogenase (quia), dehydroquinate dehydratase (quib), and dehydroshikimate dehyratase (quic), genes for catabolism of protocatechuate are encoded by the pca operon in the acinetobacter calcoaceticus chromosome. observations reported here demonstrate that a. calcoaceticus qui genes are clustered in the order quibcxa directly downstream from the pca operon. sequence comparisons indicate that quix encodes a porin ...19957592351
distance between alleles as a determinant of linkage in natural transformation of acinetobacter calcoaceticus.cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor acinetobacter calcoaceticus dna. a different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. ligation of unlinked chromosomal dna fragments allowed them to be linked genetically through natural transformation.19957592360
characterization of the extracellular lipase, lipa, of acinetobacter calcoaceticus bd413 and sequence analysis of the cloned structural gene.the extracellular lipase from acinetobacter calcoaceticus bd413 was purified to homogeneity, via hydrophobic-interaction fast performance liquid chromatography (fplc), from cultures grown in mineral medium with hexadecane as the sole carbon source. the enzyme has an apparent molecular mass of 32 kda on sds-polyacrylamide gels and hydrolyses long acyl chain p-nitrophenol (pnp) esters, like pnp palmitate (pnpp), with optimal activity between ph 7.8 and 8.8. additionally, the enzyme shows activity ...19957596283
mixed meningitis: association of acinetobacter calcoaceticus var lwoffi and streptococcus faecium.we describe mixed bacterial meningitis in a young man due to acinetobacter calcoaceticus var lwoffi and streptococcus faecium. the combination of a calcoaceticus var lwoffi and s faecium as aetiology of mixed bacterial meningitis has not been previously reported. the patient recovered completely without neurologic sequelae on chloramphenicol and penicillin.19957596936
degradation of trans-ferulic and p-coumaric acid by acinetobacter calcoaceticus dsm 586.cell suspensions of acinetobacter calcoaceticus strain dsm 586 and dsm 590 were able to grow on benzoic, p-hydroxybenzoic and vanillic acid as sole carbon source. testing the utilization of trans-ferulic and p-coumaric acid, we found that the sole a. calcoaceticus dsm 586 efficiently degraded the lignocellulose related monomers. cells induced with trans-ferulic acid were able to oxidize trans-ferulic, p-coumaric, vanillic, p-hydroxybenzoic and protocatechuic acid at rates higher than the uninduc ...19957599157
[analysis of 1116 strains of pathogens isolated from infected burn wounds].we report the analysis of 1,116 strains of pathogens isolated from infected burn wounds of 536 patients hospitalized from 1989 to 1991. from the 1,116 strains of pathogens, 39 species of aerobes and fungi were found, including 217 strains of staphylococcus aureus, 208 strains of pseudomonas aeruginosa and 119 strains of acinetobacter calcoaceticus. the positive rates of the above three bacteria were 19.4%, 18.6% and 10.6% respectively. some opportunistic pathogens, such as bacillus cercus, aeroc ...19957600434
a transposon-like sequence adjacent to the acci restriction-modification operon.we have cloned and sequenced the accirm genes from weeksella zoohelcum (the original identification of this strain as acinetobacter calcoaceticus was incorrect). our sequence differs in the coding regions from a previously published sequence by the addition of three nucleotides near the 3' end of the dna methyltransferase-encoding gene (accim). we have sequenced approx. 3 kb beyond this operon. two genes were found, convergently transcribed with the r-m operon. the first of these genes encodes a ...19957607529
novel bioemulsifiers from microorganisms for use in foods.the main objective of this study was to test the range of microorganisms for production of extracellular, high molecular weight emulsifiers for potential use in foods. a standard emulsification assay developed specifically for assessing food emulsifiers was used to examine 24 extracellular microbial products from bacteria, yeasts and algae. of the 24 products tested, nine had emulsification ability that was as good as and eight had emulsifying properties that were better than those of the common ...19957632394
purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a klebsiella pneumoniae mutant strain.unlike the parent wild-type strain, the klebsiella pneumoniae mutant strain mao4 has a 4-hba+ phenotype. the capacity of this mutant to take up and metabolize 4-hydroxybenzoate (4-hba) relies on the expression of a permease and an nadph-linked monooxygenase (4-hba-3-hydroxylase). both enzymes are normally expressed at basal levels, and only the presence of 4-hba in the media enhances their activities. strikingly, when the acinetobacter calcoaceticus poba gene encoding 4-hydroxybenzoate-3-hydroxy ...19957646318
genomic species typing of acinetobacters by polymerase chain reaction amplification of the reca gene.the reca gene has been used as a target in screening for the presence of acinetobacters on the genospecies level and differentiation of relevant acinetobacter species from one another by pcr. primers deduced from known reca gene sequences of acinetobacter calcoaceticus and neisseria gonorrhoeae allowed the amplification of dnas from all acinetobacter genospecies. the size of the amplified dna fragment from all genospecies tested was approximately 435-500 bp relative to dna size markers. the ampl ...19957649456
a case of acinetobacter calcoaceticus pneumonia.a case of community acquired pneumonia with acinetobacter calcoaceticus is presented. acinetobacter must be considered in the differential diagnosis of gram negative coccobacillary pneumonia.19957660350
phosphate uptake by immobilized acinetobacter calcoaceticus cells in a full scale activated sludge plant.an in situ study of the p-uptake ability of acinetobacter calcoaceticus was carried out using the alginate immobilization technique. immobilized a. calcoaceticus cells displayed a high p-uptake ability (> 97% p-accumulating cells) when immersed in the aerobic zone of an activated sludge system for 30-240 min. the overall p-accumulation pattern of the anaerobic zone depicted a typical p-release mechanism. however, limited p-accumulation was also observed at this stage. growth and anaerobiosis wer ...19957662295
two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in acinetobacter calcoaceticus adp1.mutants of acinetobacter calcoaceticus adp1 unable to grow on dodecane, but retaining the ability to grow on lauric acid were isolated after ethylmethanesulphonate (ems) treatment. this growth deficiency was complemented by a clone from a gene library constructed from chromosomal dna of the wild-type strain. the complementing dna mapped in a gene encoding a polypeptide with homology to rubredoxins. the deduced putative rubredoxin amino acid sequence is more similar to related proteins from gram- ...19957670642
nk-lysin, a novel effector peptide of cytotoxic t and nk cells. structure and cdna cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity.a 78 residue antimicrobial, basic peptide, nk-lysin, with three intrachain disulfide bonds was purified from pig small intestine and characterized. a corresponding clone was isolated from a porcine bone marrow cdna library. the 780 bp dna sequence had a reading frame of 129 amino acids which corresponded to nk-lysin. the clone was used to show that stimulation with human interleukin-2 induced synthesis of nk-lysin-specific mrna in a lymphocyte fraction enriched for t and nk cells. lower levels o ...19957737114
a periplasmic insulin-cleaving proteinase (icp) from acinetobacter calcoaceticus sharing properties with protease iii from escherichia coli and ide from eucaryotes.a periplasmic insulin-cleaving proteinase (icp), purified to its electrophoretic homogeneity in the sds-page from the gram-negative bacterium acinetobacter calcoaceticus, was examined and compared in its properties with the protease iii (protease pi, pitrilysin, ec 3.4.99.44) of escherichia coli and the insulin-destroying proteinase (ide, insulinase, ec 3.4.99.45) from eucaryotes. the enzyme was proven to be a metalloprotease like protease iii and ide, as was shown by the inhibitory effects exer ...19957738784
different relative importances of the par operons and the effect of conjugal transfer on the maintenance of intact promiscuous plasmid rk2.the par region of the broad-host-range, incp alpha plasmid rk2 has been implicated as a stability determinant by its ability to enhance the maintenance of mini-rk2 plasmids or heterologous replicons in a growing population of host cells. the region consists of two operons: parcba, which encodes a multimer resolution system, and parde, which specifies a postsegregational response mechanism that is toxic to plasmidless segregants. to assess the importance of this region to the stable maintenance o ...19957751288
thermostable chimeric pqq glucose dehydrogenase.the thermal stability of pqq glucose dehydrogenases (pqqgdhs) which were chimeras with more than 95% made up of the n-terminal region of escherichia coli pqqgdh and the rest made up of the c-terminal region of acinetobacter calcoaceticus pqqgdh was investigated. among the chimeric pqqgdhs, e97a3 (e. coli 97% and a. calcoaceticus 3%) and e95a5 were found to possess higher thermal stability than parental e. coli pqqgdh. further detailed characterization of the thermal stability was carried out, fo ...19957758590
acinetobacter calcoaceticus liberates chromosomal dna during induction of competence by cell lysis.a transformation assay was used to assay the amount of dna present in the extracellular medium of a growing culture of acinetobacter calcoaceticus. it was observed that small amounts of dna were liberated during the entire exponential growth phase in a batch culture. release of dna could be fully accounted for by lysis of cells. lysis was quantified via simultaneous measurement of beta-galactosidase activity of cells and supernatant, with a strain that contained a plasmid (papa100) with lacz und ...19957765885
the glucose dehydrogenase-mediated energization of acinetobacter calcoaceticus as a tool for evaluating its susceptibility to, and defence against, hazardous chemicals.cells of acinetobacter calcoaceticus 69-v could be energized by glucose oxidation after the growth on acetate, ethanol, hexanol and benzoate. the velocities of glucose oxidation-driven atp syntheses were relatively constant in the range from ph 5.4 to 7.5. with decreasing ph values (7.0, 6.0, 5.4) atp synthesis was inhibited more strongly by the action of 2,4-dinitrophenol and at the same ph value glucose oxidation was nearly unimpaired or inhibited more weakly. this finding is expressed by a de ...19957765916
characterization of lipase-deficient mutants of acinetobacter calcoaceticus bd413: identification of a periplasmic lipase chaperone essential for the production of extracellular lipase.acinetobacter calcoaceticus bd413 produces an extracellular lipase, which is encoded by the lipa gene. five lipase-deficient mutants have been generated via random insertion mutagenesis. phenotypic characterization of these mutants revealed the presence of as many as four lipolytic enzymes in a. calcoaceticus. biochemical evidence classified four of the mutants as export mutants, which presumably are defective in translocation of the lipase across the outer membrane. the additional mutant, desig ...19957768830
elucidation of the region responsible for edta tolerance in pqq glucose dehydrogenases by constructing escherichia coli and acinetobacter calcoaceticus chimeric enzymes.we constructed various chimeric pqq glucose dehydrogenases (pqqgdhs) from an edta-sensitive pqqgdh from escherichia coli and an edta-tolerant pqqgdh from acinetobacter calcoaceticus by homologous recombination of their structural genes. the edta tolerance of the resulting chimeric enzymes was investigated. our results demonstrated that edta tolerance of pqqgdhs can be completely altered by substituting each corresponding region. the edta tolerance of a. calcoaceticus pqqgdh is mostly within a re ...19957779095
molecular typing of acinetobacter baumannii-acinetobacter calcoaceticus complex isolates from endemic and epidemic nosocomial infections.ribotype, biotype and resistance phenotype were used to characterize 37 acinetobacter baumannii-a. calcoaceticus complex isolates responsible for nosocomial infections in buenos aires. nineteen isolates were recovered from endemic infections at 2 hospitals and 18 represent an intensive care unit outbreak that occurred in a third hospital. by ribotyping isolates were classified into five different clones of a. baumannii biotype 2, 3 of a. baumannii biotype 9, and 3 of acinetobacter genospecies 13 ...19957867730
spontaneous mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase in acinetobacter calcoaceticus.bacteria containing spontaneous null mutations in pcah and -g, structural genes for protocatechuate 3,4-dioxygenase, were selected by exposure of an acinetobacter calcoaceticus strain to physiological conditions in which expression of the genes prevents growth. the parental bacterial strain exhibits high competence for natural transformation, and this procedure was used to characterize 94 independently isolated spontaneous mutations. four of the mutations were caused by integration of a newly id ...19957868609
degradation of propanil by bacterial isolates and mixed populations from a pristine lake.the microbial transformation rates of propanil, a commonly used herbicide, were investigated using water from a pristine lake in northeast georgia. microbial degradation rates were measured using natural water microflora, the natural water microflora amended with five bacterial species (aerobacter aerogenes, aeromonas hydrophila, acinetobacter calcoaceticus, proteus mirabilis, and aeromonas salmonicida) isolated from the same lake, and the five isolates individually. transformation rate constant ...19957874462
nucleotide sequences transferred by gene conversion in the bacterium acinetobacter calcoaceticus.exchange of nucleotide (nt) sequences between the catijf and pcaljf regions of the acinetobacter calcoaceticus chromosome appears to contribute to frequent repair of mutations, including removal of a tn5 insertion from pcaj. repaired nt sequences are the products of nonreciprocal genetic exchange. the length of donor catijf nt tracts recovered in repaired pcaijf dna ranged from less than 315 nt to more than 881 nt. this evidence does not distinguish gene conversion from natural transformation as ...19957883173
regulation of the hema gene during 5-aminolevulinic acid formation in pseudomonas aeruginosa.the general tetrapyrrole precursor 5-aminolevulinic acid is formed in bacteria via two different biosynthetic pathways. members of the alpha group of the proteobacteria use 5-aminolevulinic acid synthase for the condensation of succinyl-coenzyme a and glycine, while other bacteria utilize a two-step pathway from aminoacylated trna(glu). the trna-dependent pathway, involving the enzymes glutamyl-trna reductase (encoded by hema) and glutamate-1-semialdehyde-2,1-aminomutase (encoded by heml), was d ...19957883699
mathematical model of self-cycling fermentation.this article presents a mathematical model for biomass, limiting substrate, and dissolved oxygen concentrations during stable operation of self-cycling fermentation (scf). laboratory experiments using the bacterium acinetobacter calcoaceticus rag-1 and ethanol as the limiting substrate were performed to validate the model. a computer simulation developed from the model successfully matched experimental scf intracycle trends and end-of-cycle results and, most importantly, settled into an unimpose ...199518623278
analysis of the interaction between autochthonous bacteria and packaging material in pvc-bottled mineral water.a study with about 10,000 bottles produced by a mineral water company was undertaken in order to identify the causal agent of an off-odour occurrence in the bottled water. some physiological attributes of the dominant species over an 8-month period, as well as their interaction with packaging material, were investigated. pseudomonas maltophilia, p. acidovorans, acinetobacter calcoaceticus var. lowffi, frequently associated with bottles having an off-odour, seemed to play a decisive role in the p ...19947921893
evaluation of minocycline and cefuzonam for antimicrobial activity against clinical isolates.the antibacterial activity of minocycline (mino) and that of cefuzonam (czon) were assessed with clinical isolates of 19 species, and compared with that of other antibiotics. mino was highly active against methicilli-sensitive staphylococcus aureus (mssa), neisseria gonorrhoeae, moraxella (branhamella) catarrhalis, haemophilus influenzae, helicobacter pylori, flavobacterium meningosepticum, acinetobacter calcoaceticus, peptostreptococcus spp. and propionibacterium acnes, but not as effective aga ...19947933531
[susceptibilities of glucose non-fermentative gram-negative bacilli to antibiotics].glucose non-fermentative gram-negative bacilli are important nosocomial pathogens. this study concerned with susceptibilities to antibacterial agents of strains of glucose non-fermentative gram-negative bacilli that were isolated from cultures of clinical materials at 123 hospital laboratories throughout japan from september to december of 1991. the tests for susceptibilities were performed according to the disk dilution method recommended by nccls. the following bacteria were tested: pseudomona ...19947933532
[laboratory and clinical studies on combined effects of fosfomycin plus sulbactam/cefoperazone for mixed infections of mrsa and pseudomonas aeruginosa].the time-lag/sequential/step by step chemotherapy by fosfomycin plus sulbactam/cefoperazone including a short time small dose of steroid was done for 27 severely infected patients suffered from various cancer or other severe basal diseases. and the staggered "intensive" chemotherapy with added arbekacin or vancomycin to the previous staggered chemotherapy was done against 13 severely infected patients having mrsas among their infecting pathogens. 1. bacteriological effects pseudomonas aeruginosa ...19947933542
properties of acinetobacter calcoaceticus reca and its contribution to intracellular gene conversion.the acinetobacter calcoaceticus pcaj and catj genes, nearly identical in dna sequence, differ in transcriptional control and are separated by more than 20 kb of chromosomal dna. the pcaj3125 mutation is repaired frequently in organisms containing the wild-type catj gene. this high-frequency repair is eliminated in strains lacking the catj gene, which suggests that recombination between the homologous catj and pcaj genes contributes to the high-frequency repair of the pcaj3125 mutation. we report ...19947934905
community-acquired bacteremias from tunneled central intravenous lines: results from studies of a single vendor.tunneled central intravenous catheters are a common method for rendering prolonged outpatient intravenous therapy. their safety, however, has not been well studied. we conducted a retrospective evaluation of bacteremias associated with tunneled central intravenous catheters managed by a single home health care vendor during a 1-year period. all catheters were inserted in the operating room under sterile conditions. to calculate total line days, the dates of catheter insertion and removal were ob ...19947943925
[degradation of oil derivatives by acinetobacter calcoaceticus mm5].this paper describes the isolation of microorganisms from polluted heating oil. the growth of one of them has been studied (acinetobacter calcoaceticus mm5) in several linear and branched hydrocarbons as well as the effect of its growth on commercial diesel oil. acinetobacter calcoaceticus mm5 is not capable of using glucose as its only source of carbon, and it needs the presence of nitrogen and phosphorus sources to degrade any petroleum by-product.19947946119
a gene of acinetobacter calcoaceticus bd413 encodes a periplasmic peptidyl-prolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth.downstream of the acinetobacter calcoaceticus esta gene, encoding a cell-bound esterase, an open reading frame (orf) was identified, which may encode a protein with a mass of 20.4 kda. this protein shows extensive similarity to both prokaryotic and eukaryotic peptidyl-prolyl cis-trans isomerases (ppiases) of the cyclophilin sub-class, especially to the periplasmic rotamase (rota) of escherichia coli. a putative signal sequence suggests that the product of the acinetobacter gene, we termed rota, ...19947948017
inducible cell lysis system for the study of natural transformation and environmental fate of dna released by cell death.two novel conditional broad-host-range cell lysis systems have been developed for the study of natural transformation in bacteria and the environmental fate of dna released by cell death. plasmid pdkl02 consists of lysis genes s, r, and rz from bacteriophage lambda under the control of the ptac promoter. the addition of inducer to escherichia coli, acinetobacter calcoaceticus, or pseudomonas stutzeri containing plasmid pdkl02 resulted in cell lysis coincident with the release of high amounts of ...19947961508
purification and properties of a novel type of malonate decarboxylase from acinetobacter calcoaceticus.a novel type of malonate decarboxylase which is induced and located in periplasm of acinetobacter calcoaceticus grown on malonate as sole carbon and energy source was purified to homogeneity. the purified 185-kda decarboxylase was found to be an oligomer composed of three different polypeptides in a ratio of 2:1:1, designated alpha (65 kda), beta (32 kda), and gamma (25 kda). optimum ph was 6.8, while pi was 6.39. the enzyme was highly specific for malonate with km 1.4 mm. this enzyme contained ...19947961952
[hydrophobic properties of acinetobacter calcoaceticus].evaluation of hydrophobic properties of 309 strains of acinetobacter calcoaceticus was performed by a sat method. among investigated strains cultured at 22 degrees c, 103 strains were autoaggregating, representing very strong hydrophobic properties. most of strains aggregated at concentration of 0.4-1.0 m (nh4)2so4, exhibiting strong hydrophobic activities. only 38 strains during culture at 22 degrees c exhibited hydrophilic surface properties. differences between strains of anitratus variety an ...19947967921
[adhesion of acinetobacter calcoaceticus to cheek epithelial cells].adhesive properties of 309 strains of acinetobacter calcoaceticus were determined toward cells of cheek epithelium obtained from 13 healthy women. most of strains adhered in number of > 0-20 bacterial cells for one epithelial cell. adherence of a. calcoaceticus to cheek epithelium was dependent not only of variety, strain and temperature of bacterial culture, but also of individual properties of epithelial cells obtained from donors. relation between hydrophobic properties and adherence to cheek ...19947967922
[clinical evaluation of sulbactam/cefoperazone for infections during the chemotherapy of hematologic malignancy].in 28 patients with hematologic malignancy, the clinical effect and the safety of sulbactam/cefoperazone (sbt/cpz) were studied on 45 episodes during granulocytopenic stage after antineoplastic chemotherapy. the overall efficacy rate obtained with sbt/cpz in combination with other drugs was 62%. the rates for sepsis, suspected sepsis and pneumonia were 67%, 94% and 38%, respectively. the efficacy in those cases that showed granulocyte count less than 300/microliters during the course of administ ...19947990260
l-carnitine via enzyme-catalyzed oxidative kinetic resolution.l-carnitine of high optical purity was prepared via kinetic resolution using a mutant strain of acinetobacter calcoaceticus atcc 39647. this organism preferentially metabolized the d-enantiomer of the racemate to furnish l-carnitine. recovery of l-carnitine was 93%, providing a total weight yield of 46.5% in 92% enantiomeric excess. the mode of degradation of carnitine was shown to proceed via a monooxygenase-catalyzed oxidative cleavage resulting in the formation of trimethylamine and malic aci ...19948000862
bactericidal activity of cefpirome (hr 810) against 513 gram-negative bacteria isolated from blood of septicemic patients.the profile of antibacterial activity of cefpirome was compared with that of nine other antimicrobial agents against 513 gram-negative bacteria isolated from septicemic patients. all strains were evaluated for their sensitivity by disc diffusion and broth dilution tests (mic and mbc). cefpirome was compared to cefazolin, cefuroxime, ceftazidime, ceftriaxone, aztreonam, imipenem, ticarcillin, tobramycin and ciprofloxacin. among the five cephalosporins tested in this study, cefpirome was the most ...19948002094
the pca-pob supraoperonic cluster of acinetobacter calcoaceticus contains quia, the structural gene for quinate-shikimate dehydrogenase.an 18-kbp acinetobacter calcoaceticus chromosomal segment contains the pcaijfbdkchg operon, which is required for catabolism of protocatechuate, and pobsra, genes associated with conversion of p-hydroxybenzoate to protocatechuate. the genetic function of the 6.5 kbp of dna between pcag and pobs was unknown. deletions in this dna were designed by removal of fragments between restriction sites, and the deletion mutations were introduced into a. calcoaceticus by natural transformation. the mutation ...19948002591
regulation of p-hydroxybenzoate hydroxylase synthesis by pobr bound to an operator in acinetobacter calcoaceticus.pobr is a transcriptional activator required for the expression of poba, the structural gene for p-hydroxybenzoate hydroxylase. the poba and pobr genes are divergently transcribed and separated by 134 bp in the acinetobacter calcoaceticus chromosome. primer extension analysis revealed that the poba transcript begins 22 bp upstream from the structural gene and the pobr transcript begins 69 bp upstream from the regulatory gene. this arrangement requires superimposition of the -10 base pair and -35 ...19948021213
inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate.the conversion of 2-chloro-cis,cis-muconate by muconate cycloisomerase from pseudomonas putida prs2000 yielded two products which by nuclear magnetic resonance spectroscopy were identified as 2-chloro- and 5-chloromuconolactone. high-pressure liquid chromatography analyses showed the same compounds to be formed also by muconate cycloisomerases from acinetobacter calcoaceticus adp1 and pseudomonas sp. strain b13. during 2-chloro-cis,cis-muconate turnover by the enzyme from p. putida, 2-chloromuco ...19948021223
comparative serum bactericidal activities of three doses of ciprofloxacin administered intravenously.the pharmacokinetics and serum bactericidal activities of three intravenous doses of ciprofloxacin were studied comparatively in 30 patients. single 200-, 300-, and 400-mg intravenous doses of ciprofloxacin were given over 30 min to 10 patients each, and serum samples were obtained at 0.5, 1, 2, 3, 4, 8, and 12 h after the start of the infusion. serum drug concentrations were determined by high-pressure liquid chromatography. pharmacokinetic parameters were estimated by using noncompartmental an ...19948031055
regulation of spvr gene expression of salmonella virulence plasmid pkdsc50 in salmonella choleraesuis serovar choleraesuis.the expression regulation of spvr, a regulatory gene on the virulence plasmid (pkdsc50) of salmonella choleraesuis serovar choleraesuis, was investigated by spvr-lacz translational fusion. the spvr gene was found to be positively regulated by its own product, the spvr protein, and this unusual positive autoregulation was repressed by the products of spva and spvb, virulence-associated genes present downstream from the spvr gene. amino acid sequence analysis revealed that the amino-terminal regio ...19948052129
contrasting patterns of evolutionary divergence within the acinetobacter calcoaceticus pca operon.the six enzymes required for catabolism of protocatechuate to succinate and acetylcoa are encoded by the pca genes in the gram-bacterium, acinetobacter calcoaceticus. the clustered a. calcoaceticus cat genes encode an analogous set of enzymes associated with the metabolic dissimilation of catechol. the nucleotide (nt) sequences of pcaijfb and pcak, reported here, complete evidence showing that all of the pca structural genes are tightly grouped in the order pcaijfbdkchg within a single operon. t ...19948063101
characterization of the chromosomal aac(6')-ij gene of acinetobacter sp. 13 and the aac(6')-ih plasmid gene of acinetobacter baumannii.the amikacin resistance genes aac(6')-ih of acinetobacter baumannii bm2686 and aac(6')-ij of acinetobacter sp. 13 bm2689 encoding aminoglycoside 6'-n-acetyltransferases were characterized. the 441-bp coding sequences predict proteins with calculated masses of 16,698 and 16,677 da, respectively. analysis of the deduced amino acid sequences indicated that the proteins belonged to a subfamily of 6'-aminoglycoside acetyltransferase type i enzymes from gram-negative bacteria. the aac(6')-ih gene of b ...19947810994
in vitro antibacterial activity and beta-lactamase stability of sy5555, a new oral penem antibiotic.the antibacterial activity of sy5555, a new oral penem antibiotic, was compared with those of cefaclor, cefixime, and cefteram. sy5555 was more active than the comparison agents against methicillin-susceptible staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, enterococcus faecalis, citrobacter freundii, enterobacter cloacae, morganella morganii, acinetobacter calcoaceticus, clostridium spp., and bacteroides fragilis. against providencia spp., proteus spp., and haemophi ...19947811005
in-vitro activity of ampicillin/sulbactam against multiresistant acinetobacter calcoaceticus var. anitratus clinical isolates. 19947814296
acinetobacter calcoaceticus anitratus outbreak in the intensive care unit traced to a peak flow meter.a cluster of seven cases of acinetobacter caleoaceticus anitratus in a community teaching hospital intensive care unit was discovered (the seventh case was located in a step-down unit next to an infected patient recently transferred from the intensive care unit.)19947847640
microbial baeyer-villiger reaction of bicyclo[3.2.0]heptan-6-ones--a novel approach to sarkomycin a.racemic (1 alpha, 2 alpha, 5 alpha)- and (1 beta, 2 alpha, 5 beta)-2- bromobicyclo[3.2.0]heptan-6-one (rac-7, rac-10, respectively), (1 alpha, 2 alpha, 5 beta)- and (1 beta, 2 alpha, 5 beta)-2- benzyloxybicyclo[3.2.0]heptan-6-one (rac-15, rac-13, respectively), (1 beta, 2 alpha, 5 beta)-2-hydroxybicyclo[3.2.0]heptan-6-one (rac-17) and cis-bicyclo[3.2.0]hept-2-en-7-one (rac-18) were subjected to a microbial baeyer-villiger reaction by acinetobacter calcoaceticus ncib 9871. in each case both regio ...19947858964
unusual g + c content and codon usage in catijf, a segment of the ben-cat supra-operonic cluster in the acinetobacter calcoaceticus chromosome.the nucleotide (nt) sequence of a 5.3-kb dna segment containing the gram- acinetobacter calcoaceticus catbcijfd operon is reported. this information completes determination of a 16-kb nt sequence containing the twelve ben and cat structural genes encoding enzymes required for catabolism of benzoate via the beta-ketoadipate pathway. many of these genes can be traced to a common ancestry with genes from other organisms containing dna with widely divergent g + c content. the a. calcoaceticus ben an ...19948125318
isolation, phenotypic characterization, and complementation analysis of mutants of methylobacterium extorquens am1 unable to synthesize pyrroloquinoline quinone and sequences of pqqd, pqqg, and pqqc.aerobic gram-negative methylotrophs oxidize methanol to formaldehyde by using a methanol dehydrogenase that has pyrroloquinoline quinone (pqq) as a prosthetic group. seventy-two mutants which are unable to grow on methanol unless the growth medium is supplemented with pqq have been isolated in the facultative methanol utilizer methylobacterium extorquens am1. in addition, 12 previously isolated methanol oxidation mutants of m. extorquens am1 were shown to be able to grow on methanol in the prese ...19948132470
azide-resistant mutants in acinetobacter calcoaceticus a2 are defective in protein secretion.azide, an inhibitor of atpase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in acinetobacter calcoaceticus a2. two such mutants were isolated that were azide-resistant and defective in the general protein transport system. the mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40 degrees c. the existence of protein secretion mutants with a selectable phenotype may be useful for the g ...19948150267
nadp-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from acinetobacter calcoaceticus and saccharomyces cerevisiae.an nadp-dependent constitutive alcohol dehydrogenase that can oxidize hexan-1-ol was detected in several gram-positive and gram-negative eubacteria and in two yeasts. the enzyme was purified to homogeneity from acinetobacter calcoaceticus ncib 8250 and from saccharomyces cerevisiae d273-10b. the bacterial enzyme appears to be a tetramer of subunit m(r) 40,300 and the yeast enzyme appears to be a monomer of subunit m(r) 43,500. the n-terminal amino acid sequence of the bacterial enzyme has 34% id ...19948162187
combined therapy of granulocyte transfusions, intravenous opsonins and antibiotics for gram-negative pneumonia in neutropenic cancer patients.four patients with hematological malignancies, following bone marrow transplantation, who developed documented gram-negative [klebsiella pneumoniae (2), pseudomonas aeruginosa or acinetobacter calcoaceticus] pneumonia during absolute neutropenia, were treated with a combination of antimicrobial therapy, granulocyte transfusions, and high-dose intravenous immunoglobulin. the patients recovered following this regimen, including 2 who had septic shock and respiratory failure, necessitating intubati ...19948171936
udp-n-acetylglucosamine 1-carboxyvinyl-transferase from acinetobacter calcoaceticus.we have analyzed the sequence downstream of rpon from acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to udp-n-acetylglucosamine 1-carboxyvinyl-transferase (murz). multicopy plasmids encoding this enzyme conferred phosphomycin resistance to a. calcoaceticus. the polar effect of a rpon mutation on the phosphomycin resistance level suggests that murz is, in part, cotranscribed with rpon. these observations confirm that a. calcoaceticus repre ...19948181716
acquisition of apparent dna slippage structures during extensive evolutionary divergence of pcad and catd genes encoding identical catalytic activities in acinetobacter calcoaceticus.the pca operon from the gram- bacterium acinetobacter calcoaceticus encodes all of the enzymes required for catabolism of protocatechuate to common intermediary metabolites. this report presents the 2754-nucleotide (nt) sequence of a hindiii restriction fragment containing pcad, the 801-bp gene encoding beta-ketoadipate enol-lactone hydrolase i. the deduced primary structure of a. calcoaceticus pcad shares 44% amino acid (aa) sequence identity with the aligned primary structure of catd (beta-ket ...19948181753
ciprofloxacin as prophylaxis for urinary tract infection: prospective, randomized, cross-over, placebo controlled study in patients with spinal cord lesion.to evaluate the efficacy of low-dose long-term treatment with ciprofloxacin in the prevention of recurrent urinary tract infections in patients with spinal cord lesions and neurogenic bladder dysfunction, a prospective, randomized, cross-over, placebo-controlled study was performed. the study period was 12 months long, including 6 months of treatment with 100 mg. ciprofloxacin at night and 6 months of placebo treatment. the study was completed by 18 men and 3 women, median age 38 years (range 19 ...19948254783
in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin.in vitro and in vivo antibacterial activities of fk037, a new parenteral cephalosporin, were compared with those of cefpirome, ceftazidime and flomoxef. the advantages of in vitro activity of fk037 were as follows: (1) a broad-spectrum antibacterial activity, (2) the most potent activity (mic90: 25 micrograms/ml) of the cephalosporins tested against highly methicillin-resistant staphylococcus aureus (h-mrsa), (3) a strong activity against enterobacter spp. and citrobacter freundii resistant to t ...19948205935
rpon (sigma 54) is required for conversion of phenol to catechol in acinetobacter calcoaceticus.members of the sigma 54 protein family, encoded by rpon, are required for the transcription of genes associated with specialized metabolic functions. the ability to grow with phenol appears to be a specialized trait because it is expressed by few of the microorganisms that grow with catechol, the metabolic product of phenol monooxygenase. a mutation preventing the expression of phenol monooxygenase in the bacterial strain acinetobacter calcoaceticus ncib8250 was complemented by wild-type dna seg ...19948206826
crystallization and preliminary x-ray analysis of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus.x-ray quality single crystals of protocatechuate 3,4-dioxygenase from acinetobacter calcoaceticus were obtained by the hanging drop method. the intradiol dioxygenase crystallizes in the cubic space group i23 with unit cell dimensions a = b = c = 145.5 a. the dodecahedral crystals diffract to beyond 2.5 a resolution. the asymmetric unit contains one twelfth of the enzyme (alpha beta fe+3)12 complex.19948107118
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