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biphasic culture system for rapid campylobacter cultivation.we developed a biphasic culture system consisting of 4 ml of brucella agar (ba) and 6 ml of brucella broth (bb) in 25-cm2 tissue culture flasks, which were incubated in air (bb/baa) or in a gas mixture of 5% o2, 10% co2, and 85% n2 (bb/bag). these media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (fb/faa and fb/fag, respectively). highly satisfactory growth of campylobacter jejuni 301 was obtained with all mediu ...19836401980
a study of the oxygen and carbon dioxide requirements of thermophilic campylobacters.the oxygen and carbon dioxide requirements of different biotypes of thermophilic campylobacters were investigated by means of (a) quantitative studies, and (b) total growth studies. oxygen tolerance of the five test organisms differed markedly and varied with the carbon dioxide concentration. at most carbon dioxide concentrations tested, campylobacter jejuni strains nctc 11168 and nctc 11392 tolerated 21% oxygen (growth reduced), c coli nctc 11353 tolerated 15% oxygen (growth reduced), and c jej ...19836408142
diarrhea associated with rotaviruses, enterotoxigenic escherichia coli, campylobacter, and other agents in costa rican children, 1976-1981.rotaviruses, enterotoxigenic escherichia coli, salmonella, shigella, and parasites were investigated in outpatient diarrheic children, and in hospitalized diarrheic and non-diarrheic children, between january 1976 and june 1979. in outpatient cases studied within 4 days of onset of symptoms, rotaviruses were the most common agents (45.3%); e. coli heat-stable enterotoxin ranked second (13.4%); shigella was third (8.1%); salmonella was fourth (7.3%). in 63.2%, one or more enteric agents were dete ...19836337517
avian wildlife reservoir of campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. in norway.cloacal swabs from 540 wild-living birds were cultured for campylobacter fetus subsp. jejuni, yersinia spp., and salmonella spp. the carrier rates detected were as follows: c. fetus subsp. jejuni, 28.4%; yersinia spp., 1.2%; and salmonella spp., 0.8%. all birds were apparently healthy when captured. c. fetus subsp. jejuni was isolated from 11 of the 40 bird species examined. among birds inhabiting the city of oslo, the highest isolation rate was found in crows (corvus corone cornix) (89.8%), fol ...19836338824
serogroups of campylobacter jejuni, campylobacter coli, and campylobacter fetus defined by direct immunofluorescence.rabbits were inoculated with whole, formalinized campylobacter jejuni, c. coli, and c. fetus cells; the c. jejuni and c. coli immunogens were identified by their dna relatedness at the species level to the type strains of c. jejuni or c. coli. the designation c. coli was not used among the other c. jejuni strains; they were classified as hippurate-positive or hippurate-negative c. jejuni. immunoglobulin g was isolated from the antisera and labeled with fluorescein isothiocyanate. these conjugate ...19836341405
an acid extract as a common antigen in campylobacter coli and campylobacter jejuni strains.an acid extract prepared from a single strain of campylobacter jejuni reacted with rabbit antisera against 21 strains of c. jejuni and campylobacter coli. the immunogen is resistant to formalin and to exposure to 100 degrees c for 30 min. the antigenic extract may have practical applications in human serology.19836343426
[campylobacter infection in children].enteritis by campylobacter jejuni/coli is by far the most frequent clinical entity of campylobacter-infection. it may be compared to salmonella-enteritis as for its epidemiologic importance. the course of the disease may be dramatic, but can be successfully treated as soon as the pathogen is known. in prematures and newborn babies infections with campylobacter fetus ssp. fetus are life-threatening and early diagnosis is of life-saving importance. five observations of campylobacter-enteritis in c ...19836352463
isolation of campylobacter jejuni from an appendix.campylobacter jejuni skirrow biotype 1, lior serotype 8 was isolated from the appendix of an 11-year-old boy who had a 6-h history of acute abdominal pain. histological diagnosis on the appendix section was early acute appendicitis. dilute carbol fuchsin stain and indirect fluorescent antibody test performed on the appendix section also revealed the presence of campylobacter sp. the patient developed a significant bactericidal antibody titer of 1,024, providing substantial clinical evidence of t ...19836352736
evaluation of transport media for campylobacter jejuni in human fecal specimens.it is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for campylobacter jejuni. in phase 1, cary-blair medium and buffered glycerol saline did not preserve c. jejuni as well as did alkaline peptone-water (apw), modified cary-blair medium, thiogl ...19836355160
detection of campylobacter fetus in artificial insemination bulls with a transport enrichment medium.one hundred and five bulls from an artificial insemination unit were tested for the presence of campylobacter fetus subspecies venerealis. the method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. seventeen bulls (16%) were found to be either positive or suspected carriers of c. fetus at one or more sampling times. the average age of these 17 bulls was about two years greater than the average age of all the bull ...19836357410
[an infection caused by campylobacter fetus]. 19836357724
correlation of an expanded direct fluorescent-antibody system with an established passive hemagglutination system for serogrouping strains of campylobacter jejuni and campylobacter coli.rabbits were inoculated with whole, formalinized cells from eight passive hemagglutination reference strains of campylobacter. fluorescein isothiocyanate-labeled immunoglobulin g from these antisera defined seven new direct fluorescent-antibody serogroups of c. jejuni and one new serogroup of c. coli. this expanded the campylobacter direct fluorescent antibody system to include 17 serogroups of c. jejuni, 3 serogroups of c. coli, and 2 serogroups of c. fetus. we then compared the passive hemaggl ...19836358249
evaluation of filters for recovery of campylobacter jejuni from water.campylobacter jejuni has been incriminated in several large waterborne outbreaks, but it has rarely been isolated from water itself. better methodology is needed for the isolation of c. jejuni from water. we evaluated three types of 0.45-micron microporous filters and three different pore sizes of positively charged depth filters for their ability to recover c. jejuni from seeded, sterile tap and surface water. the microporous filters tested were millipore ha, gelman gn6, and zetapor. three pore ...19836360048
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?the role of enrichment culture for the isolation of campylobacter jejuni from faeces is discussed. it is concluded that enrichment culture is only necessary for those specimens where it is anticipated that the number of organisms is likely to be low. in a trial of a blood free enrichment broth (ccd broth) and the modified preston enrichment broth the latter gave significantly superior results.19836361073
enteropathogen carriage by healthy individuals living in an area with poor sanitation.faecal carriage of bacterial enteropathogens (enteropathogenic escherichia coli (epec), shigellae and salmonellae) was studied in 265 individuals: 65 infants 3-6 months of age (50 bottle-fed and 15 breast-fed), 100 school-age children 8-10 years of age and 100 adults 21-50 years of age. all were apparently healthy, did not have gastrointestinal symptoms, had not received antibiotics in the preceding fortnight and were not malnourished. enteropathogens were isolated from the faeces of 24 individu ...19836363528
comparison of selective media for isolation of campylobacter jejuni/coli.a comparison of skirrow's, butzler's, blaser's, campy-bap and preston media for campylobacter spp was made using human, animal and environmental specimens. butzler's medium gave the lowest isolation rate and preston medium, which was the most selective, the highest isolation rate. enrichment culture using preston enrichment broth gave a higher isolation rate than direct plating onto preston medium.19836822680
inflammatory abdominal aortic aneurysm infected by campylobacter fetus. 19836823082
campylobacter enteritis in 188 hospitalized patients.the clinical picture and epidemiologic characteristics of infection due to campylobacter fetus subspecies jejuni were studied in 188 patients hospitalized in finland during a three-year period. all but two patients had diarrhea; 90% had abdominal pain, fever, and fatigue; half had vomiting and headache; one third experienced electrolyte disturbances; and one fifth of the patients had other complications, most commonly pancreatitis (6%) and arthritis (5%). all age groups were affected, most usual ...19836824388
relapsing septicemia caused by campylobacter fetus subsp. fetus. 19836825036
prevalence of campylobacter jejuni in two california chicken processing plants.two federally inspected california chicken processing plants participated in campylobacter jejuni prevalence studies. twelve sampling sites were included in each of four groups. groups were based on bird age, scald water temperature, and plant sampled. scald water temperatures of 60 degrees c (140 degrees f) did not contribute to a lower prevalence of c. jejuni in edible parts, as did temperatures of 53 degrees c (127 degrees f) and 49 degrees c (120 degrees f). the feather picker and chilling t ...19836830212
house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni.a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ...19836830213
experimental campylobacter jejuni infection of adult mice.ha-icr adult mice were studied to develop an animal model for campylobacter jejuni enteritis in humans. fecal and ileal cultures made by selective and nonselective methods showed that c. jejuni and related organisms are not bowel commensals. intragastric feeding of 10(8) cfu of three different strains of c. jejuni produced infection in 100% of the animals, and infection rates were dose dependent. pretreatment with antibiotics or opiates was not necessary to induce infection. fresh isolates and s ...19836832823
culture supernatants of campylobacter jejuni induce a secretory response in jejunal segments of adult rats.culture supernatants of four campylobacter jejuni strains induced a net sodium secretory flux (plasma-lumen) and an impaired glucose transport in perfused jejunal segments of adult rats in vivo.19836832835
the association of malabsorption syndrome with a campylobacter fetus infection. 19836833780
[campylobacter fetus bacteremia].five cases of campylobacter fetus bacteremia are reported. this germ, found in blood cultures, induces high fever and is accompanied by either gastroenteritis with colitis or thrombophlebitis. other, but much rarer septic sites are the meninges and endocardium. patients already debilitated by chronic disease are more susceptible to bacteremia with campylobacter fetus, which worsens the prognosis of the infection. although non-debilitated patients can contract this infection, the prognosis here i ...19836836251
an immunopathologic study of the bovine prepuce.the prepuces of 83 bulls with macroscopically normal reproductive tracts were obtained at slaughter and microbiological, immunological, and histologic studies were done and the findings were correlated. some bulls had been vaccinated on several occasions against campylobacter fetus. mean concentrations of intrapreputial immunoglobulins (ig) in 27 bulls were igg1 - 1.8 +/- 5.2; iga - 0.16 +/- 0.15; and igm - 0.24 +/- 0.24 mg/ml. high concentrations of igg2 in some bulls precluded precise estimati ...19836836875
production of enteritis in calves by the oral inoculation of pure cultures of campylobacter fetus subspecies intestinalis.pure cultures of an isolate of campylobacter fetus subspecies intestinalis obtained from the congested small intestinal mucosa of a two-week-old calf were used to infect three milk-fed calves and three ruminating calves in two separate controlled experiments. inoculated animals all developed clinical signs which included fever (to 40 degrees c) and diarrhoea with excess clear mucus containing occasional spots of blood. c fetus subspecies intestinalis was isolated from the faeces of all infected ...19836836883
isolation of plasmids encoding tetracycline resistance from campylobacter jejuni strains isolated from simians.fifteen isolates of tetracycline-resistant campylobacter jejuni were recovered from stool samples of cynomologous monkeys (macaca fascicularis) housed at the university of washington primate research center, seattle. resistance was associated with carriage of a 38-megadalton plasmid which was transmissible to other strains of c. jejuni but not to escherichia coli. seven isolates also contained a 2.6-megadalton plasmid which was phenotypically cryptic.19836838189
isolation of campylobacter fetus subsp. jejuni from the common puffin (fratercula arctica) in norway. 19836842738
legitimacy of the names of subspecies of campylobacter fetus proposed by véron and chatelain, 1980.since the publication of the 8th edition of bergey's manual of determinative bacteriology, there has been considerable confusion concerning the names of 2 subspecies and the types of the subspecies of campylobacter fetus (smith and taylor, 1919) sebald and véron, 1980. despite the documentation of the names c. fetus subsp. fetus (smith and taylor) véron and chatelain and c. fetus subsp. venerealis (florent) véron and chatelain, which appear in the "approved lists of bacterial names", the influen ...19836847035
mycotic aneurysm of the abdominal aorta: successful management of campylobacter fetus aortitis. 19836851545
selective enrichment broth medium for isolation of campylobacter jejuni.a new selective campylobacter enrichment broth for the isolation of campylobacter jejuni has been tested with fecal specimens of human, poultry, and bovine origin. we compared the isolation rate with that from the simultaneous direct plating of a duplicate specimen. of the 380 duplicate specimens examined comparatively, we obtained a 46.3% increase in isolation of c. jejuni by using the new campylobacter enrichment broth medium.19836863504
acute hepatitis associated with campylobacter colitis.a patient with proven campylobacter fetus ss. jejuni acute colitis developed hepatocellular dysfunction, which paralleled the course of the colitis. liver biopsy showed nonspecific reactive hepatitis. other causes of acute hepatocellular damage were excluded. the patient made a complete recovery.19836863882
campylobacter enteritis in normal and immunodeficient children.campylobacter fetus subspecies jejuni (cbj) has been recently recognized as a common pathogen in bacterial gastroenteritis in children. during a period of 16 months, 51 cases of c fetus subspecies jejuni gastroenteritis were diagnosed. five of the children in whom the cases were diagnosed were previously known to be immunodeficient: two had x-linked agammaglobulinemia, one had agammaglobulinemia, one had combined immunodeficiency, and one had transient hypogammaglobulinemia. average duration of ...19836869333
ferret as a potential reservoir for human campylobacteriosis.a survey was conducted to determine the frequency of campylobacter fetus subsp jejuni in feces of ferrets purchased for use in biomedical research. over a 12-month period, 168 ferrets from 2 commercial breeders were examined by bacteriologic cultural procedures for campylobacter spp; 61% were culturally positive for c fetus subsp jejuni. in a therapeutic trial with 16 ferrets shedding c fetus subsp jejuni in feces, erythromycin given orally failed to eliminate intestinal carriage of the organism ...19836870007
susceptibility of clinical isolates of campylobacter jejuni to sixteen antimicrobial agents.the activities of 16 antimicrobial agents against 103 clinical isolates of campylobacter jejuni were tested. all the strains were susceptible to kanamycin and gentamicin. chloramphenicol, nalidixic acid, and clindamycin were active against most of the strains. more than one-third of the strains were resistant to the tetracyclines and 12.5% were resistant to erythromycin.19836870228
serum antibodies in campylobacter enteritis.a solid-phase enzyme immunoassay method for the measurement of total and class-specific serum antibodies to campylobacter jejuni was developed. the test was found to be both sensitive and specific. immunoglobulin m, g, and a antibodies were detected in about 90% of sera collected from patients recovering from campylobacter enteritis, with the pattern of appearance and decline of these antibodies conforming to that usually seen in an acute infection. a poor antibody response was noted in patients ...19836885981
campylobacter jejuni as a bacterial cause of diarrhoea in ile-ife, nigeria.a total of 495 diarrhoea and non-diarrhoea patients whose ages ranged between 5 and 39 years were examined for the presence of campylobacter jejuni, salmonella and shigella species. about 12% of the specimens from diarrhoea patients were positive for campylobacter jejuni compared with 6% and 10% for salmonella and shigella species. in contrast 2%, 0% and 1% of the samples from non-diarrhoea patients were positive for campylobacter jejuni, salmonella and shigella species respectively. most (62%) ...19836886413
the microbial evaluation of acute cellulitis.twenty consecutive patients with acute cellulitis were studied by retrospective chart review. patients with suffusion and edema, with or without vesicles, were included. patients with breaks in the skin, with focal suppurations or with concurrent antimicrobial therapy were excluded. in nineteen of the twenty patients, aspirates (95 percent) failed to grow organisms. pasteurella multocida was grown from subcutaneous aspirate and blood cultures of a single patient; he was immunocompromised by wald ...19836602690
hla-b27-negative arthritis related to campylobacter jejuni enteritis in three children and two adults.five out of 37 patients with proven campylobacter jejuni enteritis developed arthritis. two adult patients presented with classical reiter's syndrome. one of the three children had reactive arthritis, and clinical suspicion of septic arthritis could not be confirmed in two. the acute synovitis subsided usually without treatment in all patients within 3-7 days, while arthralgia persisted longer in 4 patients. hla-b27 was not present in the 5 patients with arthritis, but was found in 4 others. in ...19836605028
a case of a double infection with salmonella typhi and campylobacter fetus subspecies jejuni.a case of a double infection with salmonella typhi and campylobacter subspecies jejuni proven by isolation and serology is reported. the patient was effectively treated with erythromycin and chloramphenicol.19836612785
campylobacter-induced toxic megacolon.a case of a compromised host with myeloproliferative syndrome who presented with severe, relentless but nonbloody diarrhea, fever, severe colitis, and toxic megacolon is presented. blood cultures grew out campylobacter fetus ssp. fetus. specific treatment with erythromycin reversed the grave clinical picture to normal in a 5-day period.19836613968
[campylobacter fetus bacteremia]. 19836618327
identification and characterization of campylobacter jejuni outer membrane proteins.outer membrane proteins from isolates of campylobacter jejuni were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sarcosinate-insoluble membrane preparations were outer membrane enriched based on increased ketodeoxyoctonate concentrations, the presence of surface-exposed 125i-labeled proteins that were hydrophobic, and similarity to membrane vesicle (bleb) sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles. most isolates contained a single major band with ...19836618667
extended scheme for serotyping campylobacter jejuni: results obtained in israel from 1980 to 1981.the serotyping scheme for campylobacter jejuni previously developed in the national center for campylobacter, jerusalem, was extended by the use of 20 new sera and modified by the absorption of the sera, when necessary, with homologous boiled cultures or heterologous live cultures. the extended scheme is based on slide agglutination of live suspensions and is performed in two stages: pretesting with four pooled sera and final testing with monovalent sera. so far, 34 serotypes have been recognize ...19836619282
electron microscopy of the coccoid form of campylobacter jejuni.confluent cultures of campylobacter jejuni incubated for 24 and 48 h each were examined by electron microscopy. although the 24-h-old cells exhibited typical curved morphology, the 48-h-old cells showed rounded morphology with a loss of cell integrity. this appeared to be an autolytic process that occurred very rapidly after the culture became mature. these results confirm previous evidence that the coccoid form of this organism is a degenerate state.19836619291
[occurrence of campylobacter fetus subsp. jejuni in mice and guinea pigs from experimental animal establishments]. 19836624307
inhibitory and lethal activities of rosaramicin, erythromycin, and clindamycin against campylobacter fetus subsp jejuni and intestinalis.in a comparative study of the inhibitory and lethal effects of rosaramicin, erythromycin, and clindamycin on strains of campylobacter fetus subsp jejuni and c intestinalis, c jejuni was more readily killed by rosaramicin and clindamycin than was c intestinalis. erythromycin exhibited an equally lethal effect against both subspecies. however, it was the least active of the macrolides tested against both c jejuni and c intestinalis.19836625312
[erythema nodosum and campylobacter fetus infection]. 19836625463
isolation of campylobacter jejuni from raw milk.campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. this procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of c. jejuni in milk from bulk tanks.19836625570
enhanced isolation of campylobacter jejuni by cold enrichment in campy-thio broth.isolation of campylobacter jejuni from human feces by direct inoculation to campy-bap (scott laboratories, inc., fiskeville, r.i.) was compared with isolation after overnight enrichment at 4 degrees c in campy-thio broth followed by subculture to campy-bap. of 54 positive specimens, 19 were positive only after enrichment, and 5 were positive only on the direct plate. among 36 positive patients, 10 were detected by enrichment only and 2 by direct plating only. laboratories using campy-bap should ...19836630456
effect of ferrous sulfate, sodium metabisulfite, and sodium pyruvate on survival of campylobacter jejuni.a combination of ferrous sulfate, sodium metabisulfite and sodium pyruvate, incorporated in solid medium, maintained the characteristic morphology, motility, and viability of six isolates of campylobacter jejuni stored at room temperature and 4 degrees c for up to 20 and 30 days, respectively, under normal atmospheric conditions.19836630476
campylobacter fetus subsp. fetus in homosexual males.campylobacter fetus subsp. fetus was isolated from the stools of two homosexual males. one was asymptomatic at the time of isolation. the other presented with diarrhea. both isolates were initially grown at 42 degrees c. this organism should be included among the list of organisms that are found in homosexual males.19836630480
demonstration of a cytotoxin from campylobacter jejuni.a 48-hour culture filtrate of campylobacter jejuni was found to produce cytopathic effects on three human cell lines--that is, hela, mrc-5 and hep-2. the cytopathic effects observed include cell rounding, loss of adherence and cell death after 24-48 h of incubation. such morphological changes were observed with eight of the eleven strains of campylobacter jejuni isolated from the blood/stools of patients who suffered from either acute gastroenteritis or septicaemia. the toxic factor did not reta ...19836630575
molecular identification of surface protein antigens of campylobacter jejuni.the technique of immunoblotting was used to identify the surface protein antigens of campylobacter jejuni. polyclonal antisera were raised in rabbits to formalinized cells of a typical human fecal isolate, c. jejuni vc74. surface components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. fractions analyzed included whole cell lysates, sarcosinate-extracted outer membranes, released outer membrane blebs (fragments), isolated flagella, 0.2 m glycine-hydrochloride (ph 2 ...19836642648
simple adult rabbit model for campylobacter jejuni enteritis.we tested the usefulness of the removable intestinal tie adult rabbit diarrhea model to establish campylobacter jejuni infection in rabbits. the procedure involved ligation of the cecum, placement of a slip knot at the terminal ileum, and injection of the test inoculum into the mid-small bowel. the ends of the slip knot were externalized, and the tie was released 4 h later. fifty-five rabbits received c. jejuni, and 16 received uninoculated medium as controls. daily rectal swabs were positive fo ...19836642664
campylobacter fetus ssp. fetus bacteremia in a patient with liver cirrhosis. 19836642681
probable campylobacter fetus subsp. fetus gastroenteritis.three strains of campylobacter fetus subsp. fetus isolated from cases of gastroenteritis are reported. dna-dna hybridizations in addition to biochemical tests were used to confirm the identification of the isolates as c. fetus since all strains grew at 42 degrees c. these isolates, like other c. fetus strains, are susceptible to cephalothin and thus would not have been detected in laboratories with campylobacter isolation media containing this component.19836643676
the isolation of campylobacter jejuni from flies.living flies collected from three locations were cultured on selective medium for campylobacter spp. campylobacter jejuni was isolated from five (2.4%) of 210 flies examined. these results suggest that the potential hazard to health from the transmission of campylobacters from animals to human food by flies is small.19836644008
milk-borne campylobacter enteritis in a rural area.during november and december 1981 more than 50 residents in a village in derbyshire had an acute gastrointestinal illness. one month later a second outbreak occurred affecting another 22 people. campylobacter jejuni was isolated from 12 patients; no other gastrointestinal pathogens were identified. a case-control study showed an association with the consumption of unpasteurized milk from one particular farm. no new cases were identified for 6 months following the application of a pasteurization ...19836644009
[ulcerative colitis and campylobacter fetus ss. jejuni]. 19836645707
morphological differences in flagella in campylobacter fetus subsp. intestinalis and c. fetus subsp. jejuni.intact flagella were isolated from human pathogenic strains of campylobacter, c. fetus subsp. intestinalis and c. fetus subsp. jejuni, by the method of depamphilis and adler and examined by electron microscopy. the isolated flagella were composed of a filament, a hook, a basal body, and a large disk associated with the end of the hook region covering the basal body. the width of the hook was approximately 28 nm, somewhat greater than that of the filament (20 nm in diameter). the hook region of c ...19836645983
medium for isolating campylobacter fetus. 19836649358
isolation and enumeration of campylobacter jejuni from poultry products by a selective enrichment method.a direct selective enrichment procedure was developed for the isolation of campylobacter jejuni from poultry products. the selective enrichment medium (atb) consisted of (per liter) tryptose (20 g), yeast extract (2.5 g), sodium chloride (5 g), fbp supplement (ferrous sulfate [0.25 g], sodium metabisulfite [0.25 g], sodium pyruvate [0.25 g]), bicine (10 g), and agar (1 g). hematin solution (6.25 ml; prepared by dissolving 0.032 g of bovine hemin in 10 ml of 0.15 n sodium hydroxide solution and a ...19836651294
investigation of a waterborne outbreak of campylobacter jejuni enteritis with a serotyping scheme based on thermostable antigens.serotyping of 11 human and 2 water isolates of campylobacter jejuni associated with a waterborne outbreak revealed two serotypes among the human isolates. one of these (serotype 58) was a new serotype and was added to the serotyping scheme. serotypes were defined by using extracted thermostable antigens and passive hemagglutination titrations of both unabsorbed and cross-absorbed antisera. two water isolates of the same serotype as six human isolates provided evidence to link a contaminated wate ...19836655043
detection of menaquinone-6 and a novel methyl-substituted menaquinone-6 in campylobacter jejuni and campylobacter fetus subsp. fetus.menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-naphthoquinone) were the major isoprenoid quinones found in membrane preparations of campylobacter jejuni and campylobacter fetus subsp. fetus. by reverse-phase high-performance liquid chromatography (hplc) and thin-layer chromatography (tlc) the faster-eluting menaquinone-6 co-chromatographed with a menaquinone-6 standard. the identity of menaquinon ...19836663282
[neonatal meningitis caused by campylobacter fetus]. 19836674590
[campylobacter infection and the hemolytic-uremic syndrome].a 20-year-old man with an acute enteritis caused by campylobacter fetus developed a haemolytic-uraemic syndrome on the fifth day of his disease. the pathogenesis of the disseminated intravascular coagulopathie , the nephropathie and the haemolysis are discussed. with increasing rate of isolation of campylobacter, the connection of this infection with hus may be seen more frequently.19836676172
[isolation of campylobacter fetus ssp. jejuni from products of animal origin]. 19836680922
mycotic aortic aneurysm. a complication of campylobacter fetus septicemia.the first surviving case, to our knowledge, of a campylobacter fetus mycotic aortic aneurysm is reported. bacteremia and an ileofemoral thrombophlebitis preceded the development of the infected aneurysm, reconfirming the vascular tropism of this organism. the clinical similarity with infections caused by salmonella choleraesuis is illustrated by this case. the full recovery of our patient attests to the efficacy of extralanatomic bypass combined with long-term antibiotic therapy in the treatment ...19836687525
bovine genital campylobacteriosis (vibriosis): vaccination of experimentally infected bulls.the therapeutic efficacy of a campylobacter fetus subsp venerealis bacterin was determined in experimentally infected bulls. ten of twelve 5-year-old angus bulls became infected after being infused intrapreputially with c fetus subsp venerealis. of the 10 bulls, 6 were vaccinated with 5 ml of c fetus subsp venerealis vaccine on 2 occasions 4 weeks apart. preputial washings of the vaccinated bulls were culturally negative by the 8th week after primary vaccination. none of the 18 heifers exposed t ...19836194723
nucleic acids in the classification of campylobacters.the importance of campylobacters in human disease has stimulated improvements in the methods for identification of strains from hospitals and the environment. reliable and accurate identification depends on a sound classification for which nucleic acid analyses provide fundamental information about species relationships. studies on the genus campylobacter show that the genome dna of species have base compositions of 29 to 38 mol% g + c and molecular weights of 1.54 x 10(9) to 2.31 x 10(9). campy ...19836194994
in vitro antibacterial activity of norfloxacin (mk-0366, am-715) and other agents against gastrointestinal tract pathogens.a comparison was made of the in vitro activities of norfloxacin and of nine other orally administered antibacterial agents against 180 clinical isolates representing the bacterial species most frequently implicated in infections of the gastrointestinal tract in humans. the 90% minimal inhibitory concentrations showed norfloxacin to be 4, 15, 4, 17, 17, 17, and 33 times more active than the next best compound tested against campylobacter fetus subsp. jejuni, escherichia coli, salmonella spp., shi ...19836219622
effects of disinfectants on campylobacter jejuni.because of the increasing recognition that campylobacter jejuni is an important enteric pathogen of humans, we studied the effects of widely used disinfectants on the viability of this organism. at an inoculum size of 10(3) to 10(4) cfu/ml, 1.25 mg of hypochlorite per liter killed three strains within 1 min. at an inoculum size of 10(6) to 10(7) cfu/ml, 5 mg of hypochlorite per liter killed three strains within 15 min. killing of similar concentrations of c. jejuni and escherichia coli by hypoch ...19836305266
value of stool examination in patients with diarrhoea.findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ...19836307460
the polymicrobial origin of intestinal infections in homosexual men.to determine the microbial cause and the clinical and pathologic correlates of anorectal and intestinal symptoms in homosexually active men, we performed comprehensive microbiologic studies, anoscopy, sigmoid-oscopy, and rectal biopsy in men examined in a clinic for sexually transmitted diseases. enteric pathogens were found in 95 of 119 consecutive homosexual men with anorectal or intestinal symptoms and in 29 of 75 randomly selected homosexual men without such symptoms (p less than 0.001). the ...19836308444
application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory-acquired case of campylobacter jejuni enteritis.a frequently passaged laboratory strain of campylobacter jejuni was confirmed by serotyping on the basis of thermostable antigens and by bacterial chromosomal restriction endonuclease digests to be the causative agent of enteritis in a laboratory worker.19836317712
characterization of tetracycline resistance plasmids from campylobacter jejuni and campylobacter coli.tetracycline resistance in strains of campylobacter jejuni and campylobacter coli was mediated by plasmids. intra- and interspecies transfer was demonstrated within the genus campylobacter. buoyant densities of plasmid dnas ranged from 1.691 to 1.694 g/cm3 (31 to 33% guanine plus cytosine). restriction enzymes acci, bcli, bglii, and psti were found to be most useful for comparing the plasmids. the molecular weight of c. jejuni plasmid pmak175 was 44.7 kilobases (29 x 10(6), and the other plasmid ...19836318666
[campylobacter fetus fetus bacteremia. review of the literature apropos of 2 cases].the authors report two cases of bacteremia due to campylobacter fetus fetus (also called c. fetus intestinalis). one was a 22-year-old female, under treatment for active systemic lupus erythematosus, who died. the other was a fifty-year-old male, in a poor general condition, who had a self-limited acute febrile dysentery. in this patient, c. fetus fetus was found in stools after apparent recovery, raising the problem of healthy carriers. a review of the literature shows 64 other such cases.19836320419
campylobacter fetus ssp. fetus bacteremia a patient with liver cirrhosis. 198327709517
[enterocolitis caused by campylobacter fetus subsp. jejuni--epidemiology and hygiene with special reference to federal legislation and public health measures]. 19826213894
growth of non-campylobacter, oxidase-positive bacteria on selective campylobacter agar.a total of 67 oxidase-positive, gram-negative bacteria were tested for growth on selective campylobacter agar (blaser formulation, bbl microbiology systems, cockeysville, md.) at 42 degrees c under microaerophilic conditions. although the growth of most of these bacteria was prevented, all strains of achromobacter xylosoxidans, pseudomonas aeruginosa, pseudomonas putrefaciens, pseudomonas alcaligenes, and pseudomonas pseudoalcaligenes grew as well as campylobacter fetus subsp. jejuni.19826179961
campylobacteriosis--a "new" disease in laboratory animals.campylobacter fetus ss jejuni is now recognized as a leading cause of diarrhea in humans. with the development of proper culture techniques, c jejuni has been isolated from humans with diarrhea as frequently as salmonella or shigella species. laboratory animal models are being developed to study the pathogenesis of campylobacter-induced diarrhea; also, identification of c jejuni is occurring more frequently in a variety of laboratory animals, whether asymptomatic carriers or those with clinical ...19826186839
comparison of campypak ii with standard 5% oxygen and candle jars for growth of campylobacter jejuni from human feces.to determine optimal temperature and atmospheric conditions for isolating campylobacter jejuni from fecal specimens of humans, we studied six laboratory isolates and 19 fecal specimens that were known to contain c. jejuni. we compared incubations in 5% oxygen, the campypak ii (bbl microbiology systems, cockeysville, md.) with 6 plates per jar (cp-6) and 12 plates per jar (cp-12), and candle jars at 37 and 42 degrees c. at both temperatures, the colony sizes for the laboratory strains were larger ...19826749892
a most probable number method for estimating small numbers of campylobacters in water.a most probable number (mpn) method capable of estimating as few as ten campylobacters per 100 ml of water is described. the method gave results close to those obtained by the viable count method of miles, misra & irwin (1938) with graded suspensions of campylobacter jejuni. the method was used to test raw water samples: counts were obtained ranging from 10 to 230 campylobacters per 100 ml for 11 49 coastal and estuary water samples, and from 10 to 36 campylobacters per 100 ml for 7 of 44 river ...19826752269
[cultural demonstration of campylobacter fetus subspecies fetus in preputial rinsing samples from insemination and breeding bulls]. 19826758761
[recent studies on campylobacter fetus jejuni]. 19826764587
enrichment medium and control system for isolation of campylobacter fetus subsp. jejuni from stools.enrichment culture with a semisolid medium increased by 6% the isolation rate of campylobacter fetus subsp. jejuni. the semisolid enrichment medium was also used successfully as a transport medium for campylobacter isolates. a blood agar plate streaked with pseudomonas aeruginosa, clostridium perfringens, and a laboratory strain of campylobacter was a good control system for the microaerophilic atmosphere. good growth of all three organisms indicated satisfactory conditions for culturing campylo ...19826764764
evaluation of the campypak ii gas generator system for isolation of campylobacter fetus subsp. jejuni.the campypak ii (bbl microbiology systems, cockeysville, md.) method for isolating campylobacter fetus subsp. jejuni was evaluated with clinical specimens by comparison with an evacuation replacement procedure. of 757 specimens, 26 (3.4%) were positive for c. fetus subsp. jejuni. all 26 were recovered by both systems. no difference was found in the time necessary for isolation, except with one isolate that required 3 days for isolation with campypak ii and 2 days with the established procedure. ...19826764774
comparison of atmospheres of incubation for primary isolation of campylobacter fetus subsp. jejuni from animal specimens: 5% oxygen versus candle jar.an atmosphere with reduced oxygen tension is required for the primary isolation of campylobacter fetus subsp. jejuni. therefore, we compared use of the conventional atmosphere of 5% oxygen and 8% carbon dioxide with use of a candle jar (17% oxygen and 3% carbon dioxide) for primary isolation of c. fetus subsp. jejuni from 263 positive canine, cattle, and turkey fecal or cecal specimens. at an incubation temperature of 42 degrees c, the atmosphere with 5% oxygen resulted in more campylobacter col ...19826764778
dark-field microscopy of human feces for presumptive diagnosis of campylobacter fetus subsp. jejuni enteritis.to determine the value of direct dark-field microscopy for diagnosing enteritis due to campylobacter fetus subsp. jejuni, we examined 1,377 human fecal specimens for bacteria with typical campylobacter darting motility, leukocytes, and erythrocytes. eighty-four specimens (6.1%) grew c.fetus subsp. jejuni. of the 48 specimens showing campylobacter motility, 30 (62%) grew c. fetus subsp. jejuni. the sensitivity, specificity, and predictive value of observing campylobacter motility were 36%, 99%, a ...19826764779
the relative importance of rotavirus and other pathogens in the etiology of gastroenteritis in trinidadian children.over a 2-year period, 300 infants less than 3 years old with gastroenteritis admitted to hospitals in trinidad were investigated for the presence of certain microorganisms in the feces, along with an equal number of age- and sex-matched controls. rotavirus was detected in 23% of cases and 1% of controls; salmonella in 7% of cases and in 1% of controls; shigella in 4% of cases and in no controls and two serotypes of enteropathogenic e. coli in 7% of cases and in 2% of controls. campylobacter fetu ...19826277207
respiratory physiology and energy conservation efficiency of campylobacter jejuni.a study of the electron transport chain of the human intestinal pathogen campylobacter jejuni revealed a rich complement of b- and c-type cytochromes. two c-type cytochromes were partially purified: one, possibly an oxidase, bound carbon monoxide whereas the other, of high potential was unreactive with carbon monoxide. respiratory activities determined with membrane vesicles were 50- to 100-fold higher with formate and hydrogen than with succinate, lactate, malate, or nadh as substrates. evidenc ...19826277867
aerobic and anaerobic respiratory systems in campylobacter fetus subsp. jejuni grown in atmospheres containing hydrogen.maximum growth of campylobacter fetus subsp. jejuni, strain c-61, occurred when the cultures were incubated with shaking in atmospheres containing approximately 30% hydrogen, 5% oxygen, and 10% co2. suspensions of cells grown under these conditions consumed oxygen with formate as the substrate in the presence of 0.33 mm cyanide, which completely inhibited respiration with ascorbate-n,n,n',n'-tetramethyl-p-phenylenediamine and with lactate. spectroscopic evidence with intact cells suggested that ...19826288661
campylobacter jejuni survival in chicken meat as a function of temperature.recognition of campylobacter fetus subsp. jejuni (referred to hereafter as c. jejuni) as an important human pathogen and its isolation from meat products indicate the need for knowledge of its survival characteristics in meats. thermal death times (d-values) for a single strain and a five-strain composite were determined in 1% peptone and autoclaved ground chicken meat at temperatures ranging from 49 to 57 degrees c. survival was determined for these strains in chicken meat at 4, 23, 37, and 43 ...19826812501
prevalence and survival of campylobacter jejuni in unpasteurized milk.campylobacter jejuni was isolated from 1 to 108 (0.9%) milk samples obtained from the bulk tanks of nine grade a dairy farms and from 50 of 78 (64%) cows producing grade a milk. survival of eight campylobacter strains in unpasteurized milk (4 degrees c) varied greatly: the most tolerant strain showed a less than 2-log10 decrease in viable cells after 14 days, and the most sensitive strain showed a greater than 6-log10 decrease after 7 days. one strain was still recoverable 21 days after the inoc ...19826897503
evaluation of a commercially available campylobacter fetus subsp. jejuni antigen in the serologic diagnosis of campylobacter enteritis. 19826927227
a clinical and aetiological study of adult patients hospitalised for acute diarrhoeal disease.sixty adult patients with diarrhoea discharged from the infectious disease unit, auckland hospital in the 15 months from 1 january 1980 were reviewed. thirty had diarrhoea due to enteric organisms (campylobacter fetus 8, shigella 6, salmonella typhi 4, salmonella typhimurium 4, clostridium difficile causing pseudomembranous colitis 3). other diagnoses included ulcerative colitis and a colonic carcinoma. eighteen had no specific diagnosis. combinations of admission fever, faecal leucocytes and le ...19826952128
campylobacter jejuni: incidence in processed broilers and biotype distribution in human and broiler isolates.campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in sydney, australia. three of four flocks examined carried c. jejuni. eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes.19827201780
susceptibility testing of campylobacter fetus subsp. jejuni, using broth microdilution panels.twenty-five isolates of campylobacter fetus subsp. jejuni were tested by broth microdilution panels (sensititre; gibco diagnostics, chagrin falls, ohio) and the minimal inhibitory concentrations (mics) were compared with the corresponding mics obtained by the standard agar dilution technique. microdilution panels designed for testing gram-positive organisms were used so that erythromycin, the antibiotic of choice for this organism, could be included. the correlation with agar dilution was relati ...19827041814
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