Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| systemic and mesenteric o2 metabolism in endotoxic pigs: effect of graded hemorrhage. | normally, supply-dependency of oxygen uptake (vo2) is not demonstrable unless oxygen delivery (do2) is less than a critical value (do2crit) below which vo2 is linearly dependent upon do2. because recent evidence suggests that vo2 is pathologically supply-dependent in endotoxic or septic animals and humans, we sought to determine whether 1) pathological systemic and/or mesenteric oxygen extraction (o2ext) defects occur in a porcine model of endotoxicosis and 2) arterial lactate and ileal intramuc ... | 1991 | 1742860 |
| 1-aminocyclopropane-1-carboxylate synthase in tomato is encoded by a multigene family whose transcription is induced during fruit and floral senescence. | the key regulatory enzyme in the biosynthetic pathway of the plant hormone ethylene is 1-aminocyclopropane-1-carboxylic acid (acc) synthase (ec 4.1.1.14). it catalyzes the conversion of s-adenosylmethionine to acc, the precursor of ethylene. we isolated complementary dna sequences, ptacc2 and ptacc4, for two distinct and differentially regulated acc synthase mrnas expressed in ripe tomato fruit. the authenticity of the clones has been confirmed by expression experiments in e. coli. the predicted ... | 1991 | 1762159 |
| prevalence of antibiotic-resistant escherichia coli in danish pigs and cattle. | the present paper reports on the antibiotic resistance of e. coli isolated from danish piglets and calves in 1987-1988, and compares the results with similar investigations performed during the periods 1971-1972 and 1977-1978. rectal swabs from 52 piglets and from 78 calves were examined. all the animals studied harboured resistant e. coli. this is a significant increase compared to the previously conducted investigations. the number of strains having three or more resistance markers did not dif ... | 1991 | 1772647 |
| detection of escherichia coli heat-stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction. | a combination of selective enrichment by using immunomagnetic separation of f4 (k88)-positive escherichia coli and a nested colorimetric polymerase chain reaction (pcr) was used on crude clinical and spiked samples for determination of genes encoding heat-stable enterotoxins (sts) ia (st ia) and ib (st ib). the combination increased the sensitivity of the nested pcr compared with that of application onto crude samples. dead cells were also enriched by use of this technology, giving results that ... | 1991 | 1774239 |
| positive regulator for the expression of mba protein of the virulence plasmid, pkdsc50, of salmonella choleraesuis. | a positive regulator was identified within a 2.3 kb fragment of the 6.4 kb mouse bacteremia region (mba region) of the virulence pkdsc50 plasmid of salmonella choleraesuis. sodium dodecyl sulphate polyacrylamide gel electrophoresis showed that escherichia coli k-12 carrying the recombinant plasmids of the 2.3 kb fragment produced mba1 protein with a molecular mass of 32 kda. the recombinant plasmids carrying a 4.1 kb fragment, the other part of 6.4 kb region, produced mba2 (32 kda), mba3 (70 kda ... | 1991 | 1795622 |
| sequential release of tumour necrosis factor, platelet activating factor and eicosanoids during endotoxin shock in anaesthetized pigs: protective effects of indomethacin. | 1. the effects of indomethacin were investigated on haemodynamics, haematological and blood glucose values, and the release of tumour necrosis factor (tnf), platelet activating factor (paf) and eicosanoids in anaesthetized pigs receiving 5 micrograms kg-1 e. coli lipopolysaccharide (lps) over 60 min into the superior mesenteric artery. the animals were observed for an additional period of 2 h after the termination of lps infusion. 2. eight of the 17 animals infused with lps and not treated with ... | 1991 | 1797328 |
| cross immunoreactivity of aortic kappa-2-elastin with some antibacterial antisera. | immunological and microbial factors may lead to the formation of atherosclerotic lesions. cross immune reactions between aortic kappa-2-elastin and antisera against some antigens of gram-negative bacteria--salmonella and escherichia coli--were studied using the dot-immunobinding assay. positive results were obtained with antisera against salmonella ao, bo, co, eo, hm and escherichia coli ok(a). among immunological mechanisms leading to atherosclerosis cross immunoreactivity of aortic antigens wi ... | 1991 | 1797575 |
| impact of transfer from animal-source insulins to biosynthetic human insulin (rdna e coli) in patients with diabetes mellitus. | six hundred forty-eight patients (50.5% men; 49.5% women) with diabetes mellitus on animal-source insulin therapy for at least five years were studied. in this patient population, approximately 68.7% had type i insulin-dependent diabetes mellitus and 31.3% had type ii noninsulin-dependent diabetes mellitus, nonetheless requiring insulin therapy. patients were voluntarily transferred from animal-source insulin to biosynthetic human insulin derived by recombinant dna technology from genetically al ... | 1991 | 1799920 |
| a novel method for the purification of porcine phospholipase a2 expressed in e. coli. | porcine phospholipasea2 expressed in e. coli as a fusion protein was isolated, renatured and specifically cleaved by trypsin as described in (1). active phospholipasea2, was purified to homogeneity on a column of pbe-94 over a ph region 7.4-4.5. using this method, several phospholipase a2 mutant enzymes have now been purified in a single step and all behaved identically during chromatofocusing. the method will therefore be extremely useful not only for those interested in understanding the struc ... | 1991 | 1850264 |
| complexes of yeast adenylate kinase and nucleotides investigated by 1h nmr. | the role of one of the histidine residues present in many adenylate kinases (h36 in the porcine cytosolic enzyme) is highly disputed. we thus studied the yeast enzyme (akye) containing this his residue. akye is highly homologous to the escherichia coli enzyme (akec), a protein that is already well characterized by nmr [vetter et al. (1990) biochemistry 29, 7459-7467] and does not contain the his residue in question. in addition, discrepancies between solution structural and x-ray crystallographi ... | 1991 | 1850618 |
| identification of two neutrophil chemotactic peptides produced by porcine alveolar macrophages. | we have purified to homogeneity two distinct 10-kda proteins with potent chemotactic activity for neutrophils from porcine alveolar macrophages incubated for 24 h with escherichia coli endotoxin (lipopolysaccharide (lps), 10 micrograms/ml). neutrophil chemotactic activity in alveolar macrophage supernatants was concentrated by adsorption to sp-sephadex, and purified by cation exchange and reversed phase high performance liquid chromatography. the first peptide, alveolar macrophage chemotactic fa ... | 1991 | 1850745 |
| the amino acid sequence of the aspartate aminotransferase from baker's yeast (saccharomyces cerevisiae). | 1. the single (cytosolic) aspartate aminotransferase was purified in high yield from baker's yeast (saccharomyces cerevisiae). 2. amino-acid-sequence analysis was carried out by digestion of the protein with trypsin and with cnbr; some of the peptides produced were further subdigested with staphylococcus aureus v8 proteinase or with pepsin. peptides were sequenced by the dansyl-edman method and/or by automated gas-phase methods. the amino acid sequence obtained was complete except for a probable ... | 1991 | 1859361 |
| dna hybridization and latex agglutination for detection of heat-labile- and shiga-like toxin-producing escherichia coli in meat. | dna-hybridization and latex-agglutination tests were used for screening of a group of escherichia coli isolates for heat-labile enterotoxin (lt)- and shiga-like toxin (slt1 or vt1) -producing strains, respectively. strains tested originated from 162 meat samples (poultry, pigs and beef) chosen at random. additionally lt- and slt1-producing reference strains were tested. the dna-hybridization technique allowed screening of large numbers of strains, whereas large scale testing of strains by latex ... | 1991 | 1863526 |
| endotoxin in the conscious piglet: its effects on some general and gastrointestinal myoelectrical parameters. | the effect of an intravenous bolus injection of endotoxin, 0.1, 1 or 10 micrograms/kg, on rectal temperature, clinical appearance, haematological parameters, and on gastrointestinal electrical activity was examined in 11 conscious piglets of 4-5 weeks of age, with implanted electrodes in the antrum pylori, duodenum, jejunum and ileum. all doses resulted in a significant and dose-dependent increase in rectal temperature, in pronounced clinical signs and in distinct changes in haematological value ... | 1991 | 1882516 |
| clonal relationships among escherichia coli serogroup 06 isolates from human and animal infections. | the clonal relationship of thirty e. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. two main clones with serotypes 06 : h1 or 06 : h31, h- were identified. isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possible source for human extraintestinal escherichia coli strains. two human etec (06 : h16) and two pig isolates (06 : h10) were not related to the 06 : h1 or 06 : h31, h- e. coli clone ... | 1991 | 1884980 |
| urea-induced release of heat-labile enterotoxin from escherichia coli. | urea induces the release of heat-labile enterotoxin (lt) from cells of lt-producing escherichia coli strains. optimal conditions were defined by using the checkerboard immunoblotting system. lt release was highest when e. coli cells were incubated in 8 m urea, ph 8.0, at 37 degrees c in a water bath for 30 min. urea was more effective than polymyxin b in inducing the release of lt antigen from e. coli; the activity of lt from urea-treated cells was seven times that of lt from polymyxin b-treated ... | 1991 | 1890176 |
| [site-directed mutagenesis in the uracil-repair system. preparation of mutated forms of human alpha 2 interferon]. | the mutant forms of human ifn-alpha 2 gene are obtained by oligonucleotide-directed mutagenesis with the use of uracil-repair system. to intensify the process the procedure of the uracil-containing dna template preparation is modified. it was determined that when mutagenesis is performed in the uracil-repair system the yield of the process depends on the mutant dna-strand in vitro synthesis efficiency. it is shown that the stability of the 5'-end primer-template complex and the level of the endo ... | 1991 | 1896031 |
| [coliform bacilli which produce cytotoxins: importance in veterinary medicine and public health]. | this article reviews published data (october 1st, 1990) on 4 types of cytotoxins produced by animal and human pathogenic strains of e coli, ie, verotoxins, also named shiga-like toxins, cytotoxic necrotizing factors, haemolysin, and cytolethal distending toxin. the biological and molecular properties are described for each type of cytotoxin, the association of producing strains with animal pathology, and the role of domestic animals as reservoirs of strains pathogenic for man. | 1991 | 1897865 |
| direct cloning of sequence tagged microsatellite sites by dna affinity chromatography. | 1991 | 1923833 | |
| amrinone during porcine intraperitoneal sepsis. | seven yucatan minipigs with chronic, severe intraperitoneal sepsis were given amrinone i.v. (loading dose of 0.75 mg/kg, followed by continuous infusion of 10, 20, 40, and 80 micrograms/kg/min) during the hyperdynamic phase of sepsis. hemodynamic variables and oxygen utilization, delivery, and extraction were recorded throughout the study. pulmonary capillary wedge pressure was kept constant to ensure a fixed ventricular filling pressure. intravenous amrinone modestly augmented cardiac index wit ... | 1991 | 1934325 |
| early hemodynamic indicators of gram-negative sepsis and shock in an infant pig model. | to assess the hemodynamics of early shock and to determine to what extent hemodynamic indicators of sepsis and shock can be predictive, we evaluated data from several series of experiments (including 136 piglets) in which animals were subjected to fecal-escherichia coli peritonitis-induced septic shock. serial measurements of 39 parameters were obtained and their values taken at baseline, at hyperdynamic sepsis, and at shock were compared by analysis of variance. piglet weights ranged from 1.2 t ... | 1991 | 1941483 |
| identification of enteropathogenic escherichia coli isolated in britain as enteroaggregative or as members of a subclass of attaching-and-effacing e. coli not hybridising with the epec adherence-factor probe. | strains of escherichia coli from sporadic cases of diarrhoea and belonging to serotypes o44:h18, o55:h7, o111ab:h21, o111ab:h25 or o126:h27 were examined for virulence properties. with the exception of o111ab:h25 these are considered to be classical enteropathogenic e. coli (epec) serotypes. the strains had been isolated in britain from the faeces of children less than 3 years old. of the serotypes examined, 7 of 13 o44:h18 strains, all of 10 o111ab:h21 strains and 13 of 21 o126:h27 strains belo ... | 1991 | 1941998 |
| evidence for an ancestral core structure in nucleotide-binding proteins with the type a motif. | many proteins that bind purine nucleotide triphosphates have a type a sequence motif. only two classes of structures for such proteins are so far available from x-ray crystallography. we examined the tertiary structures of representatives of the two classes, porcine cytoplasmic adenylate kinase and escherichia coli translational elongation factor tu. comparison of the two proteins suggests that the a motif may be just one part of a larger common core structure consisting of four parallel strands ... | 1991 | 1942027 |
| [escherichia coli reactions to the action of the cationic proteins of blood leukocytes]. | the antibacterial effect of cation proteins (cp) of swine leukocytes with respect to escherichia coli strain 17 has been demonstrated in vitro. the composition and properties of these cp have been studied; as a result, a comparatively high content of basic and dicarbonic amino acids and the presence of protease activity have been established. the release of the components of the metabolic fund by e. coli under the influence of cp of swine leukocytes is indicative of changes in the permeability o ... | 1991 | 1950265 |
| three-dimensional structure of porcine procarboxypeptidase b: a structural basis of its inactivity. | procarboxypeptidase b is converted to enzymatically active carboxypeptidase b by limited proteolysis catalysed by trypsin, removing the long n-terminal activation segment of 95 amino acids. the three-dimensional crystal structure of procarboxypeptidase b from porcine pancreas has been determined at 2.3 a resolution and refined to a crystallographic r-factor of 0.169. the functional determinants of its enzymatic inactivity and of its activation by limited proteolysis have thus been unveiled. the ... | 1991 | 1989878 |
| evidence for gene duplication forming similar binding folds for nad(p)h and fad in pyridine nucleotide-dependent flavoenzymes. | for pyridine nucleotide-dependent flavoenzymes, binding both fad and nad(p)h on a single amino-acid chain, we have found a high degree of internal sequence similarity for certain regions of the fad and nad(p)h binding portions of the chain for any given protein. this was the case for a range of enzyme classes, including disulphide oxidoreductases (such as glutathione reductase, trypanothione reductase, lipoamide dehydrogenase, mercuric reductase), mono- and dioxygenases, nitrite reductase, alkyl ... | 1991 | 1995341 |
| two copies of shiga-like toxin ii-related genes common in enterohemorrhagic escherichia coli strains are responsible for the antigenic heterogeneity of the o157:h- strain e32511. | thirty-two clinical isolates of shiga-like toxin (slt)-producing escherichia coli associated with single cases or outbreaks of bloody diarrhea, hemorrhagic colitis, the hemolytic uremic syndrome, or edema disease of swine were examined for multiple copies of genes belonging to the slt-i or slt-ii toxin families. five of 19 strains that were known to produce slt-ii or to hybridize to slt-ii-specific probes by colony blot were found by southern hybridization to contain two copies of toxin genes re ... | 1991 | 1997410 |
| conversion of pig pancreas phospholipase a2 by protein engineering into enzyme active against escherichia coli treated with the bactericidal/permeability-increasing protein. | phospholipases a2 (pla-2) are conserved enzymes that can vary widely in their activity toward certain biological targets. activity of pla-2 toward escherichia coli treated with the bactericidal/permeability-increasing protein (bpi) of granulocytes has been detected only in "group ii" pla-2 (lacking cys11-cys77) and correlates with overall basicity and the presence of a cluster of basic amino acids within a variable surface region near the nh2 terminus (including residues 6, 7, 10, 11, and 15). w ... | 1991 | 1999411 |
| construction of aromatic dependent shigella flexneri 2a live vaccine candidate strains: deletion mutations in the aroa and the arod genes. | live shigella flexneri 2a vaccine candidate strains with deletion mutations in the aro genes were constructed. tn10-generated auxotrophic mutations were transduced from escherichia coli to s. flexneri 2a with bacteriophage p1cmcts. the tetracycline-sensitive derivatives of tn10 mutants obtained were selected on bochner's medium and checked by dna-dna hybridization using aroa and arod gene specific probes. the vaccine candidate strains were tested to assess the efficacy of protection in guinea-pi ... | 1991 | 2008803 |
| antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (hr 111v), a new broad-spectrum cephalosporin. | cefquinome is a new injectable aminothiazolyl cephalosporin derivative. it is stable against chromosomally and plasmid-encoded beta-lactamases and has a broad antibacterial spectrum. staphylococcus aureus, streptococci, pseudomonas aeruginosa, and members of the family enterobacteriaceae (escherichia coli, salmonella spp., klebsiella spp., enterobacter spp., citrobacter spp., and serratia marcescens) are inhibited at low concentrations. cefquinome is also active against many strains of methicill ... | 1991 | 2014969 |
| tumor necrosis factor and interleukin 1 appearance in experimental gram-negative septic shock. the effects of plasma exchange with albumin and plasma infusion. | to study the effect of plasma removal vs plasma administration on the appearance of tumor necrosis factor (tnf) and interleukin 1 in septic shock, 24 anesthetized piglets were inoculated with live escherichia coli. plasma exchange with albumin was performed in one group. fresh-frozen plasma was administered to a second group. a third group served as nontreated controls. following plasma exchange, a reduction in both tnf and interleukin 1 levels occurred, whereas plasma infusion was followed by a ... | 1991 | 2021343 |
| production of antisera selective for m1 muscarinic receptors using fusion proteins: distribution of m1 receptors in rat brain. | a fragment of the cdna encoding the third intracellular loop of the rat m1 muscarinic receptor was cloned, and the dna was expressed in escherichia coli as a fusion protein. the fusion protein was purified and utilized as an antigen to raise a polyclonal antiserum in rabbits. chinese hamster ovary cells stably transfected with the cdna encoding each of the five known subtypes of muscarinic receptor were used as tissue sources to test the antiserum. the antiserum was found to quantitatively immun ... | 1991 | 2034236 |
| cloning and expression of complementary dna encoding an antigen of trichinella spiralis. | complementary dna (cdna) encoding a 49-kda antigen of trichinella spiralis was cloned, characterized, and expressed in escherichia coli by recombinant dna methods. as a first step, 29 residues of n-terminal amino acid sequence were determined by direct analysis of highly purified antigen. mixed-sequence primers based on the amino acid sequence were then synthesized and used as primers to generate a partial cdna by the polymerase chain reaction (pcr). a nearly full-length cdna was then obtained b ... | 1991 | 2038363 |
| identification and characterization of the functional amino acids at the active center of pig liver thioltransferase by site-directed mutagenesis. | by using site-directed mutagenesis techniques, the essential amino acids at the catalytic center of porcine thioltransferase (glutaredoxin) were determined. seven oligonucleotides were designed, synthesized, and used to construct mutants, ett-c22s, ett-c25s, ett-c25a, ett-r26v, ett-k27q, ett-r26v: k27q, and ett-c78s:c82s, by altering their codons in pig liver thioltransferase cdna/m13mp18 clones. each of the thioltransferases was purified to homogeneity and its dithiol-disulfide exchange, and de ... | 1991 | 2061338 |
| [isolation of vero toxin-producing escherichia coli (vtec) from imported meats]. | one hundred and four samples of various imported meats obtained from the stores and supermarkets in matsuyama-city and iyo-city from november, 1987 to october, 1988 were examined and two strains of vero toxin-producing escherichia coli were isolated: one was from beef from australia and the other was from pork from canada. by bead-elisa, it was confirmed that the strain from beef produced vero toxin 1 (vt1) and vero toxin 2 variant (vt2vh) and the strain from pork produced vt2vh. these results s ... | 1991 | 2066599 |
| killing of nongrowing and adherent escherichia coli determines drug efficacy in device-related infections. | antimicrobial therapy of device-related infections often fails, despite the in vitro susceptibility of the infecting strain. therefore, alternative laboratory-based in vitro tests are required to predict the outcome. fleroxacin, ciprofloxacin, aztreonam, and co-trimoxazole were tested against escherichia coli atcc 25922 in vitro and in the tissue-cage animal model. the importance of early treatment was evaluated by starting the drugs either 30 min before or 4, 12, and 24 h after bacterial challe ... | 1991 | 2069381 |
| nmr spectra of exchangeable protons of pyridoxal phosphate-dependent enzymes. | we have recorded 1h nmr spectra in h2o for exchangeable protons of four pyridoxal phosphate-dependent enzymes: d-serine dehydratase, aspartate aminotransferase, tryptophan: indole-lyase and glutamate decarboxylase. the molecular masses range from 48-250 kda. in every case there are downfield peaks which are lost when the apoenzyme is formed. in most cases some peaks shift in response to interactions with substrates and inhibitors and with changes in ph. we associate one downfield resonance with ... | 1991 | 2069576 |
| nucleotide sequence of the promoter and fadb gene of the fadba operon and primary structure of the multifunctional fatty acid oxidation protein from escherichia coli. | the primary structure of a multifunctional protein, the large alpha-subunit of the escherichia coli fatty acid oxidation complex, was determined by sequencing the fadb region of the fadba operon. the amino-terminal sequence of this protein had been established by edman degradation. the transcription start site of the fadba operon was located 42 nucleotides upstream of the initiator codon of the fadb gene by primer extension analysis. sequences of -10 and -35 regions of the promoter responsible f ... | 1991 | 1712230 |
| localization on the mitochondrial f1 atpase alpha subunit of an epitope masked in the membrane-bound enzyme using a monoclonal antibody and synthetic peptides. | the epitope of the monoclonal antibody 20d6 was localized by n-terminal sequencing of the smallest immunoreactive peptides obtained after cnbr and trypsin cleavage of the f1 alpha subunit of the mitochondrial atpase/atp synthase. immunochemical analysis of overlapping synthetic octapeptides, covering the immunoreactive peptide sequence, has defined the seven-amino-acid sequence recognized by 20d6 as 84egdivkr90. the binding of 20d6 was lost after substituting either i87 by k or s, or r90 by c or ... | 1991 | 1714390 |
| molecular cloning and expression of the cdna coding for a new member of the s100 protein family from porcine cardiac muscle. | we isolated a new calcium-binding protein from porcine cardiac muscle by calcium-dependent hydrophobic and dye-affinity chromatography. it showed an apparent molecular weight of 11,000 on sds-page. amino acid sequence determination revealed that the protein contained two calcium-binding domains of the ef-hand motif. the cdna gene coding for this protein was cloned from the porcine lung cdna library. sequence analysis of the cloned cdna showed that the protein was composed of 99 amino acid residu ... | 1991 | 1722468 |
| use of antibodies against the p36 protein of mycoplasma hyopneumoniae for the identification of m. hyopneumoniae strains. | mycoplasma hyopneumoniae, the principal aetiological agent of porcine enzootic pneumonia, synthesizes a 36 kda protein (p36) which is an early and strong immunogenic factor in experimentally and naturally infected swine. polyclonal antibodies were made against the recombinant p36 protein in rabbits and used for the identification of m. hyopneumoniae by the immunoblot technique. the proteins from the m. hyopneumoniae reference strains and from 13 m. hyopneumoniae field strains isolated from natur ... | 1991 | 1723490 |
| swine and cattle enterotoxigenic escherichia coli-mediated diarrhea. development of therapies based on inhibition of bacteria-host interactions. | enterotoxigenic escherichia coli (etec) frequently occurs in diarrheal disease afflicting domestic animals. in this paper is summarized the research carried out over the last decade on the two important determinants of virulence that plays a role in the development of the infection, namely the colonizing ability of the small intestine mediated by specific fimbrial adhesins acting as lectins and the production of enterotoxins. recent progress in knowledge of the phenomenon led to alternative stra ... | 1991 | 1723693 |
| [etiological and pathogenetic aspects of diarrhea in piglets following weaning]. | in this paper aetiological and pathogenic aspects of postweaning diarrhoea are discussed. besides earlier data, particular attention is paid to recent results. except specific serotypes of escherichia coli and rotaviruses, feeding regimen as well as components in the feed are important aetiological factors. villus shortening after weaning as well as the level of villus shortening are pathogenetically important. recently recovered villi may be more sensitive to the toxic effects of escherichia co ... | 1991 | 1661032 |
| activation of intestinal brush border guanylate cyclase by aromatic disulphide compounds. | guanylate cyclase in pig intestinal brush border membranes was stimulated by certain aromatic disulphides. the most effective were 6-thioguanine disulphide [(tgs)2], 6-mercaptopurine disulphide, 6,6'-dithiodinicotinic acid, 5,5'-dithiobis-(2-nitrobenzoic acid) and 5-carboxy-2-thiouracil disulphide. (tgs)2 stimulated activity 15-fold when present at 0.1 mm. the optimum concentration for each disulphide was different, and higher concentrations were inhibitory. there was no activation by alkyl disu ... | 1991 | 1673335 |
| human vntr sequences in porcine htf-islands. | mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive dna elements known as vntrs. since a high gc-content is characteristic of most of these elements one might expect them to be clustered at least partially in cpg-islands. to address this question we have constructed a porcine liver dna noti-linkage library in puc18 using isolated htf-islands. hpaii tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and ... | 1991 | 1678358 |
| pericytes of the brain microvasculature express gamma-glutamyl transpeptidase. | the expression of gamma-glutamyl transpeptidase (ggt) is a specific property of the brain capillary endothelium that constitutes the blood-brain barrier. we report here the detection of ggt, not only in endothelial cells, but also in pericytes, demonstrating that a brain capillary-specific pericyte population exists. we raised antibodies to ggt using a porcine brain microvessel ggt-protein-a (staphylococcal protein a) fusion protein as antigen which was expressed in escherichia coli. the immunoh ... | 1991 | 1684747 |
| production of type 1 fimbriae by escherichia coli hb101. | escherichia coli hb101 is frequently used as a host in the cloning of bacterial virulence genes because of its reported lack of virulence determinants such as fimbriae, adhesins and haemagglutinins. however, passage of hb101 in standing broth culture rapidly induced the production of fimbriae which mediated adhesion to hep-2 cells and mannose-sensitive haemagglutination of human and guinea-pig erythrocytes. fimbrial serology, morphology and pilin molecular mass of 18 kda were consistent with tho ... | 1991 | 1686629 |
| [antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens]. | in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ... | 1991 | 1686823 |
| identification and mapping of an immunogenic region of mycoplasma hyopneumoniae p65 surface lipoprotein expressed in escherichia coli from a cloned genomic fragment. | a previously characterized lipid-modified amphiphilic surface protein of mycoplasma hyopneumoniae, p65, has been defined by its reaction with a surface-binding monoclonal antibody (mab) and by its exclusive partitioning into the detergent phase during triton x-114 phase fractionation (k. s. wise and m. f. kim, j. bacteriol. 169:5546-5555, 1987). in the current study, polyclonal mouse antibody (pab) to gel-purified p65 was used to identify recombinant phage plaques expressing p65-related epitopes ... | 1990 | 1695206 |
| the trat lipoprotein as a vehicle for the transport of foreign antigenic determinants to the cell surface of escherichia coli k12: structure-function relationships in the trat protein. | the trat protein is a surface-exposed lipoprotein, specified by plasmids of the incf group, that mediates serum resistance and surface exclusion. the structure and function of the trat protein determined by plasmid r6-5 was probed by genetic insertion of a foreign antigenic determinant, the c3 epitope of polio virus, at residues 61, 125, 180, 200 or 216 of the protein. the chimaeric proteins were transported to the outer membrane and, in three cases, immunoassays with an anti-c3 monoclonal antib ... | 1990 | 1704095 |
| aromatic-dependent salmonella as live vaccine presenters of foreign epitopes as inserts in flagellin. | synthetic oligonucleotides specifying amino acid sequences identified as epitopes of various foreign antigens (cholera toxin subunit b, hepatitis b surface protein and others) have been inserted at an ecorv-ecorv deletion site in a cloned salmonella flagellin gene; the resulting plasmids, when placed in flagellin-negative escherichia coli or salmonella sp. strains, caused production of flagellin expressing the epitope. if the chimeric flagellin allowed formation of flagella, the epitope was expo ... | 1990 | 1714093 |
| k-antigen identification, hemolysin production, and hemagglutination types of escherichia coli o6 strains isolated from patients with urinary tract infections. | serotyping of 1918 escherichia coli strains isolated in significant cell numbers from the urine of patients with urinary tract infections (uti) revealed the presence of 117 o6 strains. the k antigens were identified by means of k-specific phages and serological methods. the phages used included a k1 phage pool (phi 1, a-e) and the separate phages phi 2, phi 5, phi 7, phi 12 and phi 13. the presence of h antigens, type 1 fimbriae formation, hemolysin production and mannose-resistant hemagglutinat ... | 1990 | 2090152 |
| [hemolytic-uremic syndrome: association with vero cytotoxin-producing escherichia coli. an epidemiological and microbiological study on possible animal reservoirs]. | vero cytotoxin-producing strains of escherichia coli (vtec) appear to be in man associated with a range of clinical symptoms from mild non bloody diarrhoea to haemorrhagic colitis and severe haemolytic uraemic syndrome (hus). we present two patients affected by hus who were investigated to determine evidence of infection by vtec. in one of the patient free fecal cytotoxin active on vero cells was detected as well as seroconversion of vt-neutralizing antibody titre. both children were living in t ... | 1990 | 2093884 |
| synthesis and characterization of escherichia coli o18 o-polysaccharide conjugate vaccines. | nontoxic, serologically reactive o polysaccharide was derived from escherichia coli o18 lipopolysaccharide by acid hydrolysis, extraction with organic solvents, and gel filtration chromatography. oxidized o polysaccharide was covalently coupled to either pseudomonas aeruginosa toxin a or cholera toxin by using adipic acid dihydrazide as a spacer molecule in the presence of carbodiimide. the resulting conjugates were composed of approximately equal amounts of o polysaccharide and protein and were ... | 1990 | 2105272 |
| mono-hydroxyeicosatetraenoic acids during porcine endotoxemia. effect of a platelet-activating factor receptor antagonist. | infusion of endotoxin into domestic pigs induces an acute respiratory failure that has many similarities with the adult respiratory distress syndrome. we hypothesized that mono-hydroxyeicosatetraenoic acids (hete) and platelet-activating factor (paf) might be involved in this model of respiratory failure. escherichia coli endotoxin (055-b5) was infused intravenously into anesthetized young pigs at 5 micrograms/kg the first hour, followed by 2 micrograms/kg/hour for 3 hours in the presence and ab ... | 1990 | 2116563 |
| three-dimensional structures of aspartate aminotransferase from escherichia coli and its mutant enzyme at 2.5 a resolution. | the structure of escherichia coli aspartate aminotransferase complex with the inhibitor 2-methylaspartate, and that of the mutant enzyme in which an arginine was substituted for a lysine residue thereby forming a schiff base with the coenzyme pyridoxal 5'-phosphate, were determined at 2.5 a resolution, by the molecular replacement method using the known structure of pig cytosolic aspartate aminotransferase. the enzyme catalyzes the reversible transamination between l-aspartate and alpha-ketoglut ... | 1990 | 2121725 |
| protection of cattle and swine against foot-and-mouth disease, using biosynthetic peptide vaccines. | a single dose of foot-and-mouth disease (fmd) virus protein 1 (vp1) peptide, expressed in escherichia coli as a fusion protein with 190 amino acids (aa) of the le' protein of the tryptophan operon of e coli, elicited an immune response in steers sufficient to withstand the challenge of exposure to animals with acute fmd. the 58-micrograms dose of viral peptide, composed of a segment of the vp1 from the a12 strain (a12) of fmd virus (fmdv; a12-32dimer) in a tandem repeat configuration of aa137 th ... | 1990 | 2154148 |
| detection of renal scarring by dmsa scanning--an experimental study. | using the established piglet model, renal scars were produced by a combination of vesicoureteric reflux and urinary infection. the presence and extent of scarring, as determined by postmortem examination, was compared with that detected by technetium 99m dimercaptosuccinic acid (dmsa) scans performed before sacrifice. sixty female piglets (62 refluxing units) were studied. overall the sensitivity (true-positive/(true-positive + false-negative)) of dmsa scanning in detecting macroscopic scarring ... | 1990 | 2158539 |
| failure of endotoxic shock to elicit superoxide anion production in pig brain. | endotoxin shock in pigs alters cerebral blood flow regulation. this study sought 1) to evaluate global cerebral electrical function using somatosensory evoked potential and 2) to determine if superoxide anion free radical (o2-) is generated in brain following endotoxin infusion. five female pigs received e. coli endotoxin (0.1 mg/kg i.v.) and supplemental fluid to maintain cardiac output at control levels. somatosensory evoked potential was generated before and after endotoxin infusion, and nitr ... | 1990 | 2160865 |
| differences in susceptibility of inbred and outbred infant mice to enterotoxigenic escherichia coli of bovine, porcine and human origin. | infant mice from outbred swiss of1 and from inbred dba/2, c57bl/6, balb/cby and cba strains were screened for usefulness in the diarrhoea model with enterotoxigenic escherichia coli (etec) strains of bovine, porcine and human origin. mouse strains were either weakly susceptible or not susceptible to etec strains of porcine or human origin bearing antigen k88, 987p, cfa/i or cfa/ii. in contrast, some mouse strains were highly susceptible to bovine and porcine etec strains bearing k99 or f41 or bo ... | 1990 | 2179554 |
| expression and refolding of recombinant human fibroblast procathepsin d. | procathepsin d is a precursor of the human lysosomal protease cathepsin d. due to its short half-life, procathepsin d is difficult to obtain in quantities sufficient to allow structural and enzymatic studies. to obtain large quantities of this precursor, procathepsin d was expressed using the t7 promoter vector pet3a in bacteria that carry a chromosomal copy of the t7 rna polymerase gene under the control of the lac promoter. at high cell density in rich medium, basal levels of t7 rna polymerase ... | 1990 | 2180427 |
| retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family. | the elongation factor 1 alpha (ef-1 alpha) is a protein which promotes the gtp-dependent binding of aminoacyl-trna to ribosomes in the protein synthesis process. a human gene coding for ef-1 alpha has previously been cloned and sequenced along with a pseudo-gene. here, we have further analyzed the family of human ef-1 alpha genes. using an ef-1 alpha cdna as probe twelve genomic ef-1 alpha-like clones were isolated and analyzed. four of these were sequenced and found to contain ef-1 alpha retrop ... | 1990 | 2183196 |
| receptor-active glycolipids of epithelial cells of the small intestine of young and adult pigs in relation to susceptibility to infection with escherichia coli k99. | glycolipids from mucosa scrapings of small intestine of neonatal and adult pigs were tested by the thin-layer chromatogram overlay assay for the binding of escherichia coli k99. there was practically no binding to acid or non-acid glycolipids of adult pig, known to be resistant to infection with this bacterium. however, piglets, which are susceptible to infection, showed a clear binding to a doublet band in the acid glycolipid fraction. the receptor-active glycolipid was isolated and shown by ma ... | 1990 | 2185031 |
| factor xa cleavage of fusion proteins. elimination of non-specific cleavage by reversible acylation. | a method is described for the elimination of non-specific cleavage of fusion proteins by factor xa. putative non-specific cleavage sites c-terminal to lysyl residues are blocked by reversible acylation by 3,4,5,6-tetrahydrophthalic anhydride prior to cleavage. after cleavage, the acyl groups are removed quantitatively by exposure to slightly acidic conditions. this method employs no harsh reagents or conditions, and may be generally applicable to factor xa cleavage of fusion proteins. | 1990 | 2185034 |
| demonstration of enterotoxigenic escherichia coli in diarrheic broiler chicks. | an investigation was made to survey the possible presence of enterotoxigenic escherichia coli (etec) in the stools of diarrheal chicks. we analyzed two outbreaks of diarrhea in broiler chicks at two independent farms in the philippines, from which no pathogens other than escherichia coli were found. in one outbreak at farm #1, all 42 isolates produced heat-labile enterotoxin (lt), with 3 of these isolates also producing heat-stable enterotoxin (st). the o serotypes of 15 strains tested randomly ... | 1990 | 2188851 |
| construction of a bivalent oral vaccine for prevention of typhoid fever and cholera diarrhea. | a recombinant plasmid pmm-ctb containing the gene for production of the nontoxic b subunit of vibrio cholera was transferred into a safe, effective and attenuated oral vaccine ty21a strain of salmonella typhi. the resulting ty21a (pmm-ctb) could steadily produce ct-b subunit that was secreted extracellularly and had the same antigenicity as ct-b produced by v. cholera. furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the str ... | 1990 | 2190570 |
| molecular cloning and nucleotide sequence of another variant of the escherichia coli shiga-like toxin ii family. | escherichia coli strain h.i.8 (o128:b12) produces low levels of a shiga-like toxin (slt) which we have called sltiiva because of its close relationship with sltiiv. the vero cell cytotoxicity of sltiiva is neutralized by antisera against sltii and sltiiv but not by antisera against slti. these data indicate that the slt of strain h.i.8 is a member of the sltii family. since sltiiva shares with sltiiv the property of having low cytotoxicity to hela cells compared with vero cells, it is appropriat ... | 1990 | 2200845 |
| evidence for in vivo synthesis of thiamin triphosphate by cytosolic adenylate kinase in chicken skeletal muscle. | we showed previously that cytosolic adenylate kinase (ak1) purified from pig skeletal muscle catalyzes in vitro formation of thiamin triphosphate (ttp) from thiamin diphosphate (tdp) and adp in addition to atp formation from adp [shikata, h. et al. (1989) biochem. int. 18, 933-942]. to obtain evidence for in vivo synthesis of ttp by ak1, changes in ttp content and ak1 activity were determined in chicken skeletal muscle during development after hatching. thiamin phosphate metabolism in chicken sk ... | 1990 | 2229026 |
| effect of creep feeding on the incidence, duration and severity of post-weaning diarrhoea in pigs. | the effect of creep feeding on the response of pigs weaned at two or three weeks old to infection with an enteropathogenic strain of escherichia coli (o149; k91[b], k88 a, c [l]) was studied in two separate experiments. gastric intubation was adopted to regulate the intake of creep feed during the sucking period. animals were given the enteropathogenic strain either before weaning or at weaning and the course and outcome of the infection followed bacteriologically and clinically. the response of ... | 1990 | 2236922 |
| host defence and bacterial growth in fosfomycin-treated peritonitis. experimental observations in pigs. | an approximately steady state of bacterial density intraperitoneally has been observed in bacterial peritonitis. this state, which follows an initial (0-4 h) phase of rapid elimination of bacteria, was now studied in a model of porcine peritonitis. twelve pigs were intra-abdominally infected with 10(10) cfu each of escherichia coli and bacteroides fragilis. six of the pigs received no antibiotic and six were given two doses of fosfomycin (anti-aerobic), 1 g i.v., with the aim of disturbing possi ... | 1990 | 2239053 |
| [morphologic changes in the intestine of swine after infection with verotoxin-producing escherichia coli]. | spontaneous morphologic lesions are described in 12 of 66 pigs submitted for necropsy. all 12 pigs were culture positive for verotoxin-producing e. coli (vtec). 10 of them were weaned pigs, one a suckling piglet and one a fattening hog. in 6 cases e. coli serovar 0139:h1 and in one case each the serovars 0139:h40; 0138:h-; 0125ac:h27 and 0154:h- were isolated. from the fecal samples of 2 animals e. coli ont (o-group non typable):h- were cultured. macroscopically there were cyanosis, edema of the ... | 1990 | 2242731 |
| early response in neonatal septicemia. the effect of escherichia coli, streptococcus agalactiae and tumor necrosis factor on the generation of lactoferrin. | using an in vitro model, we report the early effect of escherichia coli (e. coli), streptococcus agalactiea (group b streptococci, gbs) and recombinant tumor necrosis factor alpha (tnf) on the release of lactoferrin (lf) and the generation of interleukin-1 (il-1) due to e. coli, using heparinized whole blood from healthy full-term newborns. we wanted to find a dose response relationship between lactoferrin generation on the one hand and the amount of e. coli, gbs and tnf on the other hand. in a ... | 1990 | 2248767 |
| role of tryptophan 248 in the active site of tryptophanase from escherichia coli. | tryptophan 248, located in the active site of tryptophanase from escherichia coli, has been replaced with phenylalanine by site-directed mutagenesis. judging from cd and fluorescence spectra, the global structure of the mutant enzyme was found to be the same as that of the wild-type enzyme. the binding affinity of the mutant enzyme for the coenzyme pyridoxal 5'-phosphate (plp) was reduced tenfold compared to the wild-type enzyme. kinetic analyses under plp-saturated conditions indicated that the ... | 1990 | 2260981 |
| early detection of gastrointestinal mucosal ischemia in porcine e. coli sepsis. | the aim of this study was to investigate the oxygenation of the gastrointestinal tract mucosa using indirect ph measurements in a porcine septic model (intravenous infusion of live e. coli). by means of intraluminally placed balloon catheters (tonomitior) permeable to co2, intramucosal ph (phi) was calculated using the henderson-hasselbalch equation. cardiopulmonary hemodynamics and portal blood flow were measured using swan-ganz catheters. samples were taken from the gastrointestinal tract for ... | 1990 | 2264440 |
| pulmonary capillary pressure and gas exchange after e. coli bacteremia in pigs. | in 9 goettingen minipigs we studied the effect of e. coli bacteremia on effective pulmonary capillary pressure and the longitudinal distribution of pulmonary vascular resistance. precapillary pressure gradient (dpa) was calculated as the difference between mean pulmonary artery pressure (mpp) and effective pulmonary capillary pressure (pc) (dpa = mpp-pc), postcapillary pressure gradient (dpv) as the difference between pc and left atrial pressure (dpv = pc-lap). the disturbance of pulmonary gas e ... | 1990 | 2286730 |
| streptococcus suis infection in swine. a sixteen month study. | a total of 349 isolates of streptococcus suis retrieved from different tissues from diseased pigs were examined in this study. only 48% of them could be categorized as one of serotypes 1 to 8 and 1/2. among typable isolates, serotype 2 was the most prevalent (23%), followed by serotype 3 (10%). the majority of all isolates originated from lungs, meninges/brain, and multiple tissues. forty-one percent of typable isolates and 33% of untypable isolates were retrieved in pure culture. other isolates ... | 1990 | 2306668 |
| cloning of the toxin gene from pasteurella multocida and its role in atrophic rhinitis. | the gene for the osteolytic toxin of pasteurella multocida has been cloned into a plasmid vector and expressed off its own promoter in escherichia coli. particular restriction endonucleases failed to cut the gene and regions flanking it, suggesting an a + t base ratio significantly greater than the remaining genome of p. multocida. cloned toxin was indistinguishable from the native toxin with respect to molecular mass, antigenicity and toxicity in different tests. a single intraperitoneal inject ... | 1990 | 2351955 |
| drug-susceptibility and isolation of a plasmid in haemophilus (actinobacillus) pleuropneumoniae. | we isolated 56 haemophilus (actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. two drug-resistant strains were obtained. one, named kh-265, was resistant to streptomycin (sm) and sulfonamide (sa), and the other, named kh-195, was resistant to tetracycline (tc). the minimum inhibitory concentrations (mics) of drugs for resistant strains were 100 micrograms/mliters for sm, 3200 micrograms/mliters for sa, and 12.5 micro ... | 1990 | 1968988 |
| phenotype and genotype of escherichia coli isolated from pigs with postweaning diarrhea in hungary. | a total of 205 escherichia coli isolates from 88 diarrheal weanling (4- to 10-week-old) pigs from 59 farms were tested by slide agglutination for k88, k99, f41, and 987p antigens. k88 antigen was detected in 61% of the isolates representing 60% of the pigs and 56% of the farms. k88 antigen was associated with serogroup o149 and 91% of the k88+ isolates. k99, f41, and 987p were not detected. of the k88- isolates, 70 were additionally tested by colony hybridization with dna probes for adherence fa ... | 1990 | 1970575 |
| [development and preliminary characterization of monoclonal antibodies to 987p adhesin antigen of e. coli]. | a panel of 11 monoclonal antibodies (mabs) to 987p adhesin antigen of e. coli were developed and partially characterized. they only reacted with e. coli strains bearing 987p adhesin but neither with other e. coli (k88+, k99+ and f41+) strains nor other species in family of enterobacteriaceae in if or elisa. the amount of 987p+ bacteria which could be detected by the hrpo-conjugated mab epn3 was as small as 2 x 10(3) cells/ml, and the detected rate of fecal or intestinal samples from piglets with ... | 1990 | 1971468 |
| the major subunit of escherichia coli type 1 fimbriae is not required for d-mannose-specific adhesion. | type 1 fimbriae are surface organelles on escherichia coli, which mediate specific binding to d-mannose-containing structures. these fimbriae are heteropolymers composed of a major building element, the fima protein, and small amounts of the fimf, fimg and fimh proteins. the fimh protein is uniquely responsible for the d-mannose receptor binding. in this work data are presented which indicate that the major subunit of type 1 fimbriae is dispensable for d-mannose-specific binding. a recombinant s ... | 1990 | 1972261 |
| nine dna probes for detection of toxin and adhesin genes in escherichia coli isolated from diarrhoeal disease in animals. | dna gene probes specific for genes encoding heat labile enterotoxin (lti), heat stable enterotoxins (stia, stii), vero cytotoxins (vt1, vt2), and adhesins k88 (f4), k99(f5), f41 and 987p(f6) were used to examine 873 isolates of e. coli from cases of diarrhoea (680 from pigs, 187 from cattle and six from sheep). a total of 188 were toxin gene positive and of these 84 belonged to the classical etec serogroups. of the other 104 toxin gene positive strains, 80 hybridized with the vt2 probe of which ... | 1990 | 1978952 |
| binding of k99 fimbriae of enterotoxigenic escherichia coli to pig small intestinal mucin glycopeptides. | binding of purified k99 fimbriae to cryostat sections of pig small intestine was detected. binding sites were located in the mucus layer, but not in the submucosal connective tissue. high-mr mucin glycopeptides from pig small intestine were found to bind to k99-fimbriated enterotoxigenic escherichia coli, in contrast to non-fimbriated cells. sialic acid specificity of k99 fimbriae was demonstrated by the significant reduction in binding upon desialylation of mucin glycopeptides. the binding was ... | 1990 | 1979805 |
| characterization of bovine septicemic, bovine diarrheal, and human enteroinvasive escherichia coli that hybridize with k88 and f41 accessory gene probes but do not express these adhesins. | certain dna probes derived from accessory genes of cloned k88 and f41 determinants hybridize with escherichia coli strains that express k88 or f41 and with certain other e. coli strains that do not express these antigens. we found that these probes hybridized with human enteroinvasive e. coli, and with bovine e. coli isolates which produced a fatal septicemia in experimentally infected piglets. these strains did not hybridize with probes derived from the structural subunit genes encoding the k88 ... | 1990 | 1980001 |
| synthesis, purification, and active site mutagenesis of recombinant porcine pepsinogen. | in order to carry out studies on structure and function relationships of porcine pepsinogen using site-directed mutagenesis approaches, the cdna of this zymogen was cloned, sequenced, expressed in escherichia coli, and the protein refolded, and purified to homogeneity. porcine pepsinogen cdna, obtained from a lambda gt10 cdna library of porcine stomach contains 1364 base pairs. it contains leader, pro, and pepsin regions of 14, 44, and 326 residues, respectively. in addition, it also contains 5' ... | 1989 | 2494172 |
| effects of exogenous amino acids on the multiplication of porcine escherichia coli. | the multiplication rates of 70 porcine escherichia coli strains were compared in minimal medium and in medium supplemented with aspartic acid, lysine, serine and threonine, which were the amino acids taken up during multiplication of porcine e. coli in a complex medium. the effects of these amino acids singly or in combinations and the amino acids norleucine and norvaline on the growth of porcine e. coli were studied. together, aspartic acid, threonine, serine and lysine increased the multiplica ... | 1989 | 2497578 |
| effect of chlorpromazine on experimental diarrhoea in just-weaned piglets. | in just-weaned piglets (n = 30, 3-4 weeks) diarrhoea (100%) and vomiting (66%) were provoked by inoculation with transmissible gastroenteritis virus and enterotoxigenic e. coli strains (o149: k91: k88ac; lt, sta and stb enterotoxin positive). this combined infection resulted in a mortality of 71% within 7 days. during this period animals revealed a decrease in body weight, in arterial pressure, in leukocyte count, in plasma ph and in plasma lactic acid concentrations, and an increase in heart ra ... | 1989 | 2501952 |
| a possible mechanism of the inhibitory effect of endotoxin on adenylate cyclase. | the possible mechanism of the inhibitory effect of endotoxin on adenylate cyclase was investigated. plasma membranes were isolated from porcine thyroid gland and rat liver. the effects of endotoxin on adenylate cyclase activity was determined. the adenylate cyclase activity followed in the presence of various concentrations of guanosine-imidodiphosphate (5 x 10(-8)-10(-6) m) and naf (0.1-10 mm) was markedly inhibited by endotoxin. moreover, the basal activity of adenylate cyclase was inhibited s ... | 1989 | 2518620 |
| a 32 kda lipocortin from human mononuclear cells appears to be identical with the placental inhibitor of blood coagulation. | a 32 kda protein isolated from human mononuclear cells is a member of the lipocortin family, a new group of ca2+-dependent lipid-binding proteins thought to be involved in the regulation of phospholipase a2, in exocytosis and in membrane-cytoskeleton interactions. purification of this protein was based on its ability to associate with membrane phospholipids in a ca2+-dependent manner and its capacity to inhibit purified phospholipase a2 from pig pancreas. using immunological detection, we show t ... | 1989 | 2532007 |
| comparative analysis of the genomes of intestinal spirochetes of human and animal origin. | the aim of the present work was to compare the genomes of 21 strains of intestinal spirochetes, which were isolated from patients suffering intestinal disorders, with those of treponema hyodysenteriae (strain p18), the known etiological agent of swine dysentery (bloody scours), and of a nonpathogenic strain (m1) of treponema innocens. the percent guanine-plus-cytosine value of the 23 dnas was found to be 25.5 to 30.1, as determined by a double-labeling procedure based on nick-translation by dna ... | 1989 | 2535832 |
| site-directed mutagenesis of pro-17 located in the glycine-rich region of adenylate kinase. | proline 17 in the glycine-rich region of adenylate kinase was replaced by gly (the gly-mutant) or val (the val-mutant) by site-directed mutagenesis. the mutant enzymes were purified to homogeneous states on sodium dodecyl sulfate-gel electrophoresis after solubilization of the proteins from the pellets of cell lysates of escherichia coli. the apparent km values of the gly- and the val-mutants for amp increased approximately 7- and 24-fold, respectively, as compared with that of the wild-type enz ... | 1989 | 2536029 |
| a new compound, withangulatin a, promotes type ii dna topoisomerase-mediated dna damage. | withangulatin a, a new compound with a known chemical structure and from the antitumor chinese herb physalis angulata l, was found to act on topoisomerase ii to induce topoisomerase ii-mediated dna damage in vitro. it has two effective dosage ranges of approximate 0.5 and 20 microm, with about one-third the activity of 20 microm vm-26. | 1989 | 2539141 |
| transfer of plasmid hly in vivo in pigs intestine. | the results of our study suggest the in vivo transfer of hly plasmid from native pathogenic and enterotoxigenic escherichia coli strain to autochtonous escherichia coli, using ileal loop test. to confirm this hypothesis phly::tn5 and phly::tn3 were obtained using an in vitro recombination method, and introduced to escherichia coli laboratory strain. for experiments the laboratory strain, carrying phly::tn5 and phly::tn3 and phly::tn5 strain which acquired k88(f4) by means of conjugation, were us ... | 1989 | 2550170 |
| modulatory activity of 9-hydroxy- and 9-hydroperoxy-octadecadienoic acid towards reactive oxygen species from guinea-pig pulmonary macrophages. | as guinea-pig pulmonary macrophages (pm) synthesize the linoleic acid metabolite 9-hydroxy-octadecadienoic acid (9-oh-lin) under non-stimulated conditions in relatively large quantities, we investigated whether this product has an effect on the macrophage's own phagocytic cell function. 9-oh-lin, and also its hydroperoxy precursor 9-hydroperoxy-octadecadienoic acid (9-ooh-lin), influenced the generation of pm chemiluminescence, a measure of the production of reactive oxygen species. the generati ... | 1989 | 2550227 |
| opossum kidney contains a functional receptor for the escherichia coli heat-stable enterotoxin. | the escherichia coli heat-stable enterotoxin (st1 or sta) binds to specific receptors on mammalian intestinal brush border membranes, and stimulates guanylate cyclase in those membranes. we have found a similar signal transduction system in brush border membranes prepared from kidney cortex of the american opossum (didelphis virginiana, and in a cell line (ok cell) derived from that tissue. activation of guanylate cyclase by st1 is therefore not limited to intestinal cells. furthermore, since it ... | 1989 | 2564275 |
| peptidyl-prolyl cis-trans isomerase is the cyclosporin a-binding protein cyclophilin. | peptidyl-prolyl cis-trans isomerase (ppiase) catalyses the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and has been shown to accelerate the refolding of several proteins in vitro. its activity has been detected in yeast, insects and escherichia coli as well as in mammals, and it is though to be essential for protein folding during protein synthesis in the cell. we purified ppiase from pig kidney and found that its amino-acid sequence is identical to that reported for ... | 1989 | 2644542 |
| antibodies to escherichia coli j5 core glycolipids in gnotobiotic and conventionally reared piglets. | antibody titers to escherichia coli j5 endotoxin core glycolipids were determined in 5-week-old, unvaccinated, conventionally reared and gnotobiotic pigs in a micro-elisa. both groups of pigs had naturally occurring antibody of all major isotypes. the conventionally reared pigs had significantly (p less than 0.05) more serum antibody to j5 endotoxin glycolipids (geometric mean titer, gmt = 2.07) than gnotobiotes (gmt = 0.85). this relationship was not necessarily true in fluids from various anat ... | 1989 | 2655265 |
| conjunctival oxygen tension monitoring in experimental septic shock. | continuous non-invasive monitoring of conjunctival oxygen tension (pcjo2) versus 'conventional' invasive hemodynamic and oxygen transport variables was evaluated in a porcine model of septic shock induced by a continuous i.v. infusion of e. coli endotoxin over 2 hours. seventeen pigs under ketamine anesthesia and breathing air spontaneously were investigated. pcjo2, which reflects local oxygen tension at tissue level, correlated significantly at baseline and throughout the septic course with mix ... | 1989 | 2656892 |
| complement depletion with naje haje cobra venom factor limits prostaglandin release and improves visceral perfusion in porcine endotoxic shock. | we tested the hypothesis that complement (c')-dependent release of prostaglandin (pg) i2 is an important factor contributing to the development of hypotension and low systemic vascular resistance index (svri) in endotoxic shock. two groups (n = 7) of pentobarbital-anesthetized pigs (12-15 kg) were infused over 40 min with escherichia coli lipopolysaccharide (lps; 200 micrograms/kg) and continuously resuscitated with normal saline (1 ml/kg min): lps-control (no pretreatment) and lps-decomplemente ... | 1989 | 2668544 |
| molecular cloning and characterization of chromosomal virulence region kcpa of shigella flexneri. | in shigella flexneri, in addition to several well-recognized plasmid-borne virulence loci, at least three genetic loci implicated in pathogenesis have been recognized on the chromosome. to understand more about the pathogenesis of bacillary dysentery at a molecular level, the genetically recognized but previously unidentified kcpa region (one of the chromosomal regions near pure) was cloned and sequenced. a single translatable open reading frame encoding a 12310 dalton protein corresponding to t ... | 1989 | 2668687 |