Publications

typing of human papillomavirus in women with cervical lesions: prevalence and distribution of different genotypes.	human papillomavirus (hpv) are distributed widely and persistent infection with high-risk (hr) hpv is recognized as a necessary cause of cervical cancer. the aim of this study was to evaluate the distribution of different hr-hpv genotypes in 199 women with cervical pre-invasive lesions undergoing conservative treatment. a linear array hpv genotyping test was used to identify individual hpv genotypes in cervical samples. it was observed that the most prevalent hpv genotypes were hpv 16 (52.6%), h ...	2009	19107962
genomic diversity of human papillomavirus (hpv) genotype 38.	human papillomavirus (hpv) genotype hpv 38 is a hpv genotype associated with skin cancer and is classified taxonomically in the beta-pv genus-species 2. at least six genomic variants of hpv 38, including prototype isolate and its subtype fa125, have been characterized so far. in order to investigate further the genomic diversity of hpv 38, a total of 39 hpv 38 positive samples obtained from hairs plucked from pubic, scrotal, perianal or eyebrow regions from 31 immunocompetent healthy male indivi ...	2009	19107968
tax1bp1 interacts with papillomavirus e2 and regulates e2-dependent transcription and stability.	the papillomavirus e2 proteins regulate viral replication, gene transcription, and genome maintenance by interacting with other viral and host proteins. from a yeast two-hybrid screen, we identified the cellular protein tax1bp1 as a novel binding partner of human papillomavirus type 18 (hpv18) e2. tax1bp1 also interacts with the hpv16 and bovine papillomavirus type 1 (bpv1) e2 proteins, with the c-terminal region of tax1bp1 interacting with the n-terminal transactivation domain of bpv1 e2. tax1b ...	2009	19109394
sequence variation of human papillomavirus type 16 and measurement of viral integration by quantitative pcr.	given that the integration of human papillomavirus type 16 (hpv16) into the host genome occurs preferentially with the disruption of the e2 gene, a ratio of e2 to e7 gene copies is often used as a marker for integration. it is largely undetermined, however, whether ratio estimates are affected by hpv intratypic variations. we assembled four plasmid constructs, each containing a dna fragment from an hpv16 european, asian-american, african-1, or african-2 variant. these constructs and nine cervica ...	2009	19116350
antibody response for l1, e6, e7 hpv 16, and hpv 18 antigens in tunisian women with cervical cancer.	results obtained in the present work indicated that the luminex assay is more sensitive than elisa. the reactivity to the early antigens e6 and e7 was 37% versus 42% for hpv 16 and 21% versus 20% for hpv 18 among cervical cancer cases using elisa. however, these ratios were 44% and 61%, respectively, for e6 and e7 hpv 16 versus 28% and 21% for e6 and e7 hpv 18 when using the luminex technique. data also indicated that hpv 16 and hpv 18 showed distinct profiles for the different antigens tested. ...	2009	19117204
prevaccination distribution of human papillomavirus types in women attending at cervical cancer screening in belgium.	before the introduction of vaccination against human papillomaviruses (hpv) as a new strategy of combating cervical cancer, it is required to describe the baseline prevalence of hpv infection as well as the distribution of the different hpv types in the population and among women with cervical lesions.	2009	19124515
variables associated with human papillomavirus (hpv) vaccine acceptance by men.	to determine correlates of human papillomavirus (hpv) vaccine acceptance for men.	2009	19124631
detection of human papillomavirus dna in retinoblastoma samples: a preliminary study.	recent studies have shown the presence of human papillomavirus (hpv) genome in retinoblastoma (rb) tumor samples. there is no information on the hpv status in the rb tumors of indian patients. we studied the presence of hpv genome in rb tumor samples from patients with unilateral tumor. forty-four fresh rb tumor samples and 30 non-neoplastic donor retinas were analyzed for the presence of hpv 16 and 18 genome by nested and seminested polymerase chain reaction. tumor tissue sections were also use ...	2009	19125079
an hpv 16 l1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic t-cell activity in mice.	even though two prophylactic vaccines against hpv are currently licensed, infections by the virus continue to be a major health problem mainly in developing countries. the cost of the vaccines limits wide-scale application in poor countries. a promising strategy for producing affordable and efficient vaccines involves the expression of recombinant immunogens in plants. several hpv genes have been expressed in plants, including l1, which can self-assemble into virus-like particles. a plant-based, ...	2009	19126233
epidemiological data of different human papillomavirus genotypes in cervical specimens of hiv-1-infected women without history of cervical pathology.	to study the epidemiology of different human papillomavirus (hpv) genotypes in cervical samples of hiv-1-infected women with normal papanicolau smears.	2009	19131892
single nucleotide polymorphism array analysis defines a specific genetic fingerprint for well-differentiated cutaneous sccs.	cutaneous squamous cell carcinomas (csccs) are the second most frequent cancers in fair-skinned populations; yet, because of their genetic heterogeneity, the key molecular events in cscc tumorigenesis remain poorly defined. we have used single nucleotide polymorphism microarray analysis to examine genome-wide allelic imbalance in 60 csccs using paired non-tumor samples. the most frequent recurrent aberrations were loss of heterozygosity at 3p and 9p, observed in 39 (65%) and 45 (75%) tumors, res ...	2009	19131950
expression of human papilloma virus type 16 e5 protein in amelanotic melanoma cells regulates endo-cellular ph and restores tyrosinase activity.	melanin synthesis, the elective trait of melanocytes, is regulated by tyrosinase activity. in tyrosinase-positive amelanotic melanomas this rate limiting enzyme is inactive because of acidic endo-melanosomal ph. the e5 oncogene of the human papillomavirus type 16 is a small transmembrane protein with a weak transforming activity and a role during the early steps of viral infections. e5 has been shown to interact with 16 kda subunit c of the trans-membrane vacuolar atpase proton pump ultimately r ...	2009	19133143
palliative aspects of recurrent respiratory papillomatosis.	recurrent respiratory papillomatosis (rrp) is a chronic, frequently debilitating, and potentially life-threatening disease. therapy for rrp has evolved from simply inserting a tracheotomy to provide an airway and plucking out papillomata with cup forceps to provide some degree of voice to the present-day far more sophisticated approaches, along with preventative measures that may someday offer the potential dramatically to decrease disease prevalence. family dynamics and support and intentional ...	2009	19134490
enhancement of dendritic cell-based vaccine potency by anti-apoptotic sirnas targeting key pro-apoptotic proteins in cytotoxic cd8(+) t cell-mediated cell death.	dendritic cells (dcs) have become an important measure for the treatment of malignancies. current dc preparations, however, generate short-lived dcs because they are subject to cell death from various apoptotic pressures. antigen-specific cd8(+) cytotoxic t lymphocytes (ctls) is one of the main obstacles to limit the dc-mediated immune priming since ctls can recognize the target antigen expressing dcs as target cells and kill the dcs. ctls secret perforin and serine protease granzymes during ctl ...	2009	19135479
asymmetric gp5+/6+ pcr and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples.	the detection of a broad spectrum of hpv genotypes has not been used widely in cervical cancer screening due to technical difficulties and high costs.	2009	19144564
molecular hpv typing as a diagnostic tool to discriminate primary from metastatic squamous cell carcinoma of the lung.	in this study, typing of human papilloma virus (hpv) was performed in squamous cell carcinomas of the lung (n=26) as well as putative primaries of head and neck (n=21) and female genital tract (n=5) of the same patients, to test whether additional information to discriminate lung primaries from metastases can be gained by a direct comparison of the hpv status in both tumors. in 3 (14.2%) patients with head and neck as well as lung squamous cell carcinoma, an identical hpv subtype could be detect ...	2009	19145203
prevalence of human papillomavirus genotypes in women from a rural region of puebla, mexico.	to determine the prevalence of human papillomavirus (hpv) genotypes 6, 11, 16, 18, and 31 in mexican women living in rural areas of puebla, mexico and to evaluate risk factors associated with cervical neoplasm in this population.	2009	19147384
chromosomal signatures of a subset of high-grade premalignant cervical lesions closely resemble invasive carcinomas.	cervical cancer develops from precancerous high-grade cervical intraepithelial neoplasia (cin) harboring a transforming infection with high-risk human papillomavirus, which is characterized by p16(ink4a) overexpression. once such a lesion has developed, progression toward an invasive squamous cell carcinoma (scc) may take one or more decades, underlining the heterogeneity of these lesions in terms of duration of existence and progression risk. we performed array-based comparative genomic hybridi ...	2009	19147580
anticancer effects on tacc3 by treatment of paclitaxel in hpv-18 positive cervical carcinoma cells.	previously, we used proteome analysis to identify transforming acidic coiled coil (tacc) 3 as a protein that is down-regulated upon paclitaxel treatment in cervical cancer cells. tacc3 mrna and protein levels decreased after paclitaxel treatment in a time- and dose-dependent manner, and the transactivation of tacc3 promoter was dramatically diminished by paclitaxel. importantly, paclitaxel treatment and knockdown of tacc3 by sirna led to a synergistic enhancement of significant g2/m phase arrest ...	2009	19148534
interaction between human papillomavirus type 5 e2 and polo-like kinase 1.	the e2 protein of the papillomavirus plays an essential role in the viral life cycle. through a yeast two-hybrid screening, human polo-like kinase 1 was found to interact with human papillomavirus type 5 e2. further characterization identified that the domains responsible for the interaction are the transactivation domain of hpv-5 e2 and the sequence between the kinase and the polo box domains of plk1. in vivo, plk1 and hpv-5 e2 are colocalized at the nuclear speckles. in the skin epithelium not ...	2009	19152411
high-risk hpv types in lesions of the uterine cervix of female commercial sex workers in the philippines.	in order to prevent cervical cancer, vaccines against human papilloma virus types 16 (hpv-16) and 18 (hpv-18) have been implemented worldwide. however, the hpv types that cause cancer can differ according to geographical area and ethnicity. in this new era of the hpv vaccine, it is important to elucidate the prevalent hpv types in each area. therefore, the prevalence of hpv infection and cervical abnormalities among 369 female commercial sex workers in the philippines were examined. hpv l1 gene ...	2009	19152419
gene expression patterns induced by hpv-16 l1 virus-like particles in leukocytes from vaccine recipients.	human papillomavirus (hpv) virus-like particle (vlp) vaccines were recently licensed. although neutralizing ab titers are thought to be the main effectors of protection against infection, early predictors of long-term efficacy are not yet defined and a comprehensive understanding of innate and adaptive immune responses to vaccination is still lacking. here, microarrays were used to compare the gene expression signature in hpv-16 l1 vlp-stimulated pbmcs from 17 vaccine and 4 placebo recipients be ...	2009	19155521
a strained dna binding helix is conserved for site recognition, folding nucleation, and conformational modulation.	nucleic acid recognition is often mediated by alpha-helices or disordered regions that fold into alpha-helix on binding. a peptide bearing the dna recognition helix of hpv16 e2 displays type ii polyproline (pii) structure as judged by ph, temperature, and solvent effects on the cd spectra. nmr experiments indicate that the canonical alpha-helix is stabilized at the n-terminus, while the pii forms at the c-terminus half of the peptide. re-examination of the dihedral angles of the dna binding heli ...	2009	19156829
characterization of immortalized human epidermolysis bullosa simplex (krt5) cell lines: trimethylamine n-oxide protects the keratin cytoskeleton against disruptive stress condition.	epidermolysis bullosa simplex (ebs) is an autosomal inherited mechano-bullous disease, characterized by intraepidermal blistering and skin fragility caused by mutations in the keratin (krt) 5 or 14 genes. despite a vast knowledge about the intermediate filament pathology in this disease, the progress in therapy has been slow. animal models and well-characterized continuous cell culture models of ebs are needed prior to clinical testing.	2009	19157792
a type-specific study of human papillomavirus prevalence in cervicovaginal samples in three different spanish regions.	human papillomavirus (hpv) is the most frequent sexually transmitted viral infection. it is necessary to know hpv genotype distribution to identify how many women will be protected by hpv vaccines. during a period of 18 months, we have analyzed 2362 hpv positive reporting data from a secondary demand screening program in three regions in spain (cantabria, leon and burgos). the study has been conducted using polymerase chain reaction and tube array hybridization covering the 35 hpv genotypes desc ...	2009	19161533
induction of focal epithelial hyperplasia in tongue of young bk6-e6/e7 hpv16 transgenic mice.	squamous cell carcinoma (scc) of the oral cavity is one of the most common neoplasms in the world. during the past 2 decades, the role of high-risk human papilloma virus (hr-hpv) has been studied and the data supporting hpv as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (hnscc) has accumulated. in order to investigate the role of hr-hpv oncogene expression in early epithelial alterations in vivo, we produced transgenic m ...	2009	19165615
the bcl-xl inhibitor of apoptosis is preferentially expressed in cutaneous squamous cell carcinoma compared with that in keratoacanthoma.	keratoacanthoma (ka) is difficult to histologically distinguish from squamous cell carcinoma (scc). therefore, although ka is a benign self-resolving skin lesion, ka is commonly treated as scc. biomarkers to distinguish ka and scc would thus be desirable. in search for specific markers, paraffin-embedded tissue samples from 25 scc and 64 ka were arranged in a tissue microarray (tma) and stained for immunologic cell-markers cd3, cd20 and cd68 as well as for proteins considered of relevance in tum ...	2009	19165861
triage of women with equivocal or low-grade cervical cytology results: a meta-analysis of the hpv test positivity rate.	consistent evidence underlines the utility of human papillomavirus (hpv) dna testing in the management of women with equivocal cervical cytological abnormalities, but not in case of low-grade lesions. we performed a meta-analysis including studies where the high-risk probe of the hybrid capture-ii is used to triage these two cytological categories. the triage test-positivity rate reflects the colposcopy referral workload.data were pooled on the hpv test positivity rate in women with atypical squ ...	2009	19166485
prognostic value of measuring load of human papillomavirus dna in cervical samples: an elusive target.		2009	19176455
[mechanisms of high-risk human papillomavirus-induced cervical carcinogenesis].	human papillomaviruses (hpvs) are believed to be the primary causal agents for development of pre-neoplastic and malignant lesions of the uterine cervix and high-risk types such as type 16 and 18 are associated with more than 90% of all cervical carcinomas. the e6 and e7 genes of hpvs are thought to play causative roles, since e6 promotes the degradation of p53 through its interaction with e6ap, an e3 ubiquitin ligase, whereas e7 binds to the retinoblastoma protein prb and disrupts its complex f ...	2009	19177752
[advancements in diagnosis of sexually transmitted diseases in urology].	sexually transmitted diseases diagnosed and treated exclusively in urology clinics are male urethritis and epididymitis caused by neisseria gonorrhoeae and chlamydia trachomatis. although recent developments in diagnostic tools have made it possible to detect these microorganisms with extremely high sensitivities, the screening of neisseria gonorrhoeae using the microscopic observation of intraleucocyte diplococci stained by methylene blue or gram staining in the first voided urine sediment or u ...	2009	19177755
epithelial expression of human papillomavirus type 16 e7 protein results in peripheral cd8 t-cell suppression mediated by cd4+cd25+ t cells.	the role of thymic versus peripheral epithelium in the regulation of the antigen-specific cd8 t-cell repertoire is still largely unresolved. we generated tcr-beta chain transgenic mice in which an increased frequency of peripheral cd8 t cells recognizes an epitope from a viral oncoprotein (hpv16e7) in the context of h-2d(b) mhc class i. when t cells from these mice developed through the thymus of mice expressing functional e7 protein from a keratin 14 promoter, no major perturbation to transgeni ...	2009	19180468
multiple human papillomavirus infection and high grade cervical intraepithelial neoplasia among women with cytological diagnosis of atypical squamous cells of undetermined significance or low grade squamous intraepithelial lesions.	to evaluate the effect of multiple human papillomavirus (hpv) infection on the prevalence of cervical intraepithelial neoplasia (cin) among women undergoing colposcopy following a cytological diagnosis of atypical squamous cells of undetermined significance (ascus) or low grade squamous intraepithelial lesions (lsil).	2009	19181368
immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells.	most in vitro studies of oral carcinogenesis in human cells are carried out with oral keratinocytes immortalized by human papillomavirus type 16 dna. however, because various etiological factors for oral cancer are known, it is important to establish new human keratinocyte cell lines useful for studying the mechanism of oral carcinogenesis. normal human gingival keratinocytes in secondary cultures grown in serum-free medium were either transfected with origin (-) sv40 dna or sequentially transfe ...	2009	19184294
ensembled support vector machines for human papillomavirus risk type prediction from protein secondary structures.	infection by the human papillomavirus (hpv) is regarded as the major risk factor in the development of cervical cancer. detection of high-risk hpv is important for understanding its oncogenic mechanisms and for developing novel clinical tools for its diagnosis, treatment, and prevention. several methods are available to predict the risk types for hpv protein sequences. nevertheless, no tools can achieve a universally good performance for all domains, including hpv and nor do they provide confide ...	2009	19185855
genetic variants in tap are associated with high-grade cervical neoplasia.	the transporter associated with antigen processing (tap) is essential in assembling mhc-i proteins. human papillomavirus (hpv) evades immune recognition by decreasing class i mhc cell surface expression through down-regulation of tap1 levels. consistent with heterogeneity in mhc expression is the individual variability in clearing detectable hpv infections. genetic polymorphisms in tap genes may affect protein structure, function, and the ability to clear hpv infection.	2009	19188174
human papillomavirus type distribution in anal cancer and anal intraepithelial lesions.	a systematic review was conducted of hpv type distribution in anal cancer and anal high-grade and low-grade squamous intraepithelial lesions (hsil and lsil). a medline search of studies using pcr or hybrid capture for hpv dna detection was completed. a total of 1,824 cases were included: 992 invasive anal cancers, 472 hsil cases and 360 lsil cases. crude hpv prevalence in anal cancer, hsil, and lsil was 71, 91 and 88%, respectively. hpv16/18 prevalence was 72% in invasive anal cancer, 69% in hsi ...	2009	19189402
disparities in how parents are learning about the human papillomavirus vaccine.	differential access to basic health information may contribute to persistent cervical cancer disparities. we examined whether human papillomavirus (hpv) vaccine awareness, hpv knowledge, and use of information sources about the vaccine differ by sociodemographic characteristics associated with cervical cancer.	2009	19190161
[human papillomavirus and cancer of the oropharynx. molecular interaction and clinical implications].	one-third of the cases of oropharyngeal squamous cell carcinoma (oscc) contain oncogenic human papillomavirus (hr-hpv). epidemiologic and molecular evidence underlines the causal role of hr-hpv in these tumors, which can be defined as hpv-related oscc. these tumors differ from chemical/toxin-induced oscc in several biological aspects, including specific molecular and genetic alterations. this leads to a characteristic clinical profile of hpv-related oscc. sexual risk factors play a role; however ...	2009	19194683
[assessing the incidence of papillomavirus infections in france].	high-risk oncogenic human papillomavirus (hr-hpv) are strongly associated with squamous cell carcinoma and adenocarcinoma of the uterine cervix. relatively detailed data on prevalence of hr-hpv infections by age in france are available in several cytologically normal women populations but none on incidence. these latter data may be obtained according to the relationship between prevalence and incidence due to the spontaneous viral clearance over time. the study has shown that hr-hpv infections i ...	2009	19196534
human papillomavirus-associated squamous cell carcinoma of the nail bed in african-american patients.	human papillomavirus (hpv) has been implicated in the development of digital squamous cell carcinoma (scc). case reports in the literature mostly identify hpv type 16 present in tumors, but hpv types 2, 31, 34, 35, and 73 have also been isolated.	2009	19200182
an e7-based therapeutic vaccine protects mice against hpv16 associated cancer.	plant-derived vaccines represent an attractive strategy for cancer immunotherapy due to their relative safety and cost-effectiveness. we evaluated the anti-tumour activity of a nicotiana benthamiana produced vaccine candidate based on the non-transforming e7 protein of hpv-16 fused to beta-1,3-1,4-glucanase of clostridium thermocellum. two doses of vaccine at two week intervals were administered to groups of c57bl/6 mice starting 3 or 6 days after challenge with tumourigenic e7-expressing tc-1* ...	2009	19200826
prophylactic hpv vaccination for women over 18 years of age.	cervical screening has resulted in a major reduction in the incidence and mortality of cervical cancer in the uk and other developed countries. nevertheless approximately 2700 women present with cervical cancer in the uk each year with mortality in excess of 1000 cases. prophylactic hpv vaccination against hpv 16 and 18 has been shown to be highly effective in preventing hpv related malignancy in clinical trials. newly introduced hpv vaccination programmes in the uk and elsewhere are ultimately ...	2009	19200838
vaginal self-swab specimen collection in a home-based survey of older women: methods and applications.	to describe the methods used for, cooperation with, assays conducted on, and applications of vaginal specimens collected by older women in their homes.	2009	19204072
high-risk and multiple human papillomavirus (hpv) infections in cancer-free jamaican women.	vaccines, that target human papillomavirus (hpv) high risk genotypes 16 and 18, have recently been developed. this study was aimed at determining genotypes commonly found in high-risk and multiple-hpv infections in jamaican women. two hundred and fifty three (253) women were enrolled in the study. of these, 120 pregnant women, aged 15-44 years, were recruited from the ante natal clinic at the university hospital of the west indies and 116 non-pregnant, aged 19-83, from a family practice in weste ...	2009	19208202
comparisons of high-risk cervical hpv infections in caribbean and us populations.	disparities in cervical cancer incidence and mortality rates exist among women of african ancestry (african-american, african-caribbean and african). persistent cervical infection with human papillomavirus (hpv) is associated with cervical dysplasia and if untreated, could potentially progress to invasive cervical cancer. very few studies have been conducted to examine the true prevalence of hpv infection in this population. comparisons of cervical hpv infection and the type-specific distributio ...	2009	19208214
statbite: prevalence of hpv in a cohort of u.s. men.		2009	19211451
cellular growth inhibition by fk778 is linked to g1 arrest or s phase accumulation, dependent on the functional status of the retinoblastoma protein.	the malononitrilamide fk778 is a novel immunosuppressive agent with antiproliferative activities. to gain insight into the molecular mechanism of fk778-mediated growth inhibition, we analyzed cells which differ in their p53 status and functionality of retinoblastoma protein (prb). fk778 acted as a broad inhibitor of cell proliferation independent of the p53 or prb status. however, the mechanism of fk778-mediated growth inhibition differed, leading either to cell cycle arrest in g1, or cell accum ...	2009	19212661
cancer prevention by vaccination against hepatitis b.	chronic inflammation caused by persistent infection is closely related to a number of cancers; these include hepatitis b (hbv) or c and hepatoma, human papilloma virus and cervical cancer, and helicobacter pylori and gastric cancer. the first evidence of cancer prevention by vaccination in humans was provided by hbv vaccination in infants. chronic hbv is related to approximately 60%-90% of hepatocellular carcinomas (hcc) in adults and nearly 100% of childhood hcc in areas endemic for hbv infecti ...	2009	19213561
human papillomaviruses and genital co-infections in gynaecological outpatients.	high grade hpv infections and persistence are the strongest risk factors for cervical cancer. nevertheless other genital microorganisms may be involved in the progression of hpv associated lesions.	2009	19216747
surgical salvage of cancer of the oropharynx after chemoradiation.	advanced oropharyngeal squamous cell carcinoma is treated primarily with chemoradiation, with the goal of excellent disease control and preservation of swallowing and articulation functions of the oropharynx. disease control rates generally are excellent; however, a significant number of patients do not achieve locoregional control of disease. the importance of human papillomavirus expression in predicting successful tumor response, locoregional control, and survival following chemoradiation is ...	2009	19216847
genetic signatures of hpv-related and unrelated oropharyngeal carcinoma and their prognostic implications.	patients with human papillomavirus (hpv)-containing oropharyngeal squamous cell carcinomas (oscc) have a better prognosis than patients with hpv-negative oscc. this may be attributed to different genetic pathways promoting cancer.	2009	19223504
knowledge and awareness of cervical cancer and screening among malaysian women who have never had a pap smear: a qualitative study.	cervical cancer is one of the commonest cancers in women worldwide. despite the existence of effective screening using pap smear, the uptake of screening is poor. a qualitative study was undertaken using face-to-face in-depth interviews to investigate knowledge, attitudes and beliefs on cervical cancer screening of malaysian women.	2009	19224084
screening for oral cancer.	the incidence of oral cancer appears to be increasing, especially in younger patients. risk factors include use of tobacco and alcohol, and exposure to human papillomavirus. vizilite plus (zila pharmaceuticals) is a combination device that uses fluorescent light and toluidine blue tissue staining to help dentists identify abnormal changes in the mucous membranes of the oral cavity. originally developed for detecting abnormal growths on the uterine cervix, in 2001 it received fda clearance for "i ...	2009	19229163
ifng (+874 t/a) polymorphism and cervical intraepithelial neoplasia in brazilian women.	our objective was to investigate the relationship between ifng (+874 t/a) polymorphism and cervical intraepithelial neoplasia (cin) in a population of brazilian women. ninety-six women, cin ii (48) and cin iii (48) and 50 normal controls, were enrolled in this study. dna was extracted from blood samples by the salting out method and polymerase chain reaction (pcr) amplified. ifng genotyping was performed by the pcr-sequence specific primer method, using the cytokine genotyping tray. there were n ...	2009	19232001
cervical infections by multiple human papillomavirus (hpv) genotypes: prevalence and impact on the risk of precancerous epithelial lesions.	a large proportion of human papillomavirus (hpv) infections is sustained by multiple genotypes. the effect of multiple infections on the risk of cervical intraepithelial neoplasia (cin) and the potential efficacy of vaccine on these infections are controversial. we performed viral typing by sfp(10)-lipa on a consecutive series of 1,323 women undergoing colposcopy, 69% of whom had cervical biopsy, and correlated cin severity with the type and number of hpvs. overall prevalence of hpv-dna was 68.9 ...	2009	19235847
quantitative human papillomavirus 16 and 18 levels in incident infections and cervical lesion development.	human papillomavirus (hpv) rna levels may be a more sensitive early indicator of predisposition to carcinogenesis than dna levels. we evaluated whether levels of hpv-16 and hpv-18 dna and messenger rna (mrna) in newly detected infections are associated with cervical lesion development. female university students were recruited from 1990 to 2004. cervical samples for hpv dna, hpv mrna, and papanicolaou testing were collected tri-annually, and women were referred for colposcopically directed biops ...	2009	19235870
molecular detection and genotyping of human papillomavirus in cervical carcinoma biopsies in an area of high incidence of cancer from moroccan women.	cervical cancer is a leading cause of cancer-related deaths in developing countries, and the human papillomavirus (hpv) is linked etiologically to cervical cancer. eighty nine cervical carcinoma biopsies collected from women visiting the oncologic center in casablanca (centre hospitalier universitaire ibn rochd, morocco) for cervical cancer symptoms, were screened for hpv dna by polymerase chain reaction amplification with subsequent typing by hybridization with specific oligonucleotides for hpv ...	2009	19235879
human papillomavirus (hpv) vaccines: limited cross-protection against additional hpv types.		2009	19236278
comparing knowledge and perceived risk related to the human papilloma virus among australian women of diverse sexual orientations.	the study compared levels of awareness of human papilloma virus (hpv) as a sexually transmissible infection (sti) between women of different sexual orientations. it also examined self-reported risk factors for hpv infection, perceived level of personal risk, and willingness to have the hpv vaccine.	2009	19236366
vulval intraepithelial neoplasia with mucinous differentiation: report of 2 cases of a hitherto undescribed phenomenon.	we report 2 cases of classic vulval intraepithelial neoplasia (vin) with collections of cells throughout the full epithelial thickness and within epithelial downgrowths containing intracytoplasmic mucin. the mucinous cells were bland with eccentrically placed small regular nuclei and were positive with cam5.2, carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 7, markers that are typically positive in the neoplastic cells of paget disease. both cases were strongly positive wi ...	2009	19238078
school nurses' workloads: how should they be prioritised?	the school nurse role has developed considerably over the past two decades in order to meet the changing health needs of the school-age population. changes demand continuous adaptability to prioritise workload pressures, one example of which is the new vaccination programme against human papillomavirus. this paper examines the wider context in which school health care is delivered and proposes a systematic, evidence-based approach that school nurse managers could take toward prioritising tasks. ...	2009	19238818
prevalence, clearance, and incidence of anal human papillomavirus infection in hiv-infected men: the hipvirg cohort study.	human immunodeficiency virus (hiv)-seropositive men who have sex with men (msm) are at higher risk of human papillomavirus (hpv) infection. this study was conducted to better understand the natural history of type-specific hpv infection in the anus.	2009	19239366
proteomic analysis reveals the actin cytoskeleton as cellular target for the human papillomavirus type 8.	recent studies strongly support a role of human papillomavirus type 8 (hpv8) in non-melanoma skin cancer development. in this study, a quantitative two-dimensional (2d) differential gene expression (dige) gel approach combined with mass spectrometry has been used to identify proteins that are abundantly deregulated in primary human epidermal keratinocytes expressing hpv8 sequences. twenty six protein spots showed significant changes in the level of expression between keratinocytes expressing e7 ...	2009	19243805
determinants of stability for the e6 protein of papillomavirus type 16.	e6 is an oncoprotein produced by human papillomavirus (hpv). the e6 protein from high-risk hpv type 16 contains two zinc-binding domains with two c-x-x-c motifs each. e6 exerts its transforming functions through formation of a complex with e6ap, which binds p53 and stimulates its degradation. there have been few biophysical and structural studies due to difficulty in preparation of soluble protein; here we describe the preparation of soluble e6 constructs including the two separated zinc-binding ...	2009	19244625
currents in contemporary ethics: hpv vaccines, privacy, and public health.		2009	19245609
molecular targets for cancer chemoprevention.	vaccines targeting infections with hepatitis b virus, a risk factor for hepatocellular cancer, and human papillomavirus, a risk factor for cervical cancer, are considered major clinical cancer chemoprevention successes. molecularly targeted agents can prevent breast cancer (raloxifene and tamoxifen), colorectal adenomas (celecoxib), and prostate cancer (finasteride). nevertheless, the broad translation of chemoprevention to the clinic is not yet a reality. continuing research of molecular target ...	2009	19247304
human papillomavirus negative but dyskaryotic cervical cytology: re-analysis of molecular testing.	evaluation of molecular human papillomavirus (hpv) testing into uk cervical screening programmes is underway. in south wales the current hpv prevalence in women attending routine screening is 13.5% with 76.3% hr hpv positive in cases with reported dyskaryotic cervical cytology [hibbitts s, jones j, powell n, dallimore n, mcrea j, beer h, et al. human papillomavirus prevalence in women attending routine cervical screening in south wales, uk: a cross-sectional study. br j cancer 2008;99(december ( ...	2009	19264544
increased risk of oncogenic human papillomavirus infections and incident high-grade cervical intraepithelial neoplasia among smokers: experience from the latin american screening study.	the purpose of this study was to assess the effect of smoking on the prevalence and incidence of high-risk human papillomavirus (hr-hpv) infection and cervical intraepithelial neoplasia (cin) in a large sample of latin american women.	2009	19265732
p16, ki-67, and bd proexc immunostaining: a practical approach for diagnosis of cervical intraepithelial neoplasia.	p16, ki-67, and bd proexc (tripath imaging, inc., burlington, nc) have each been shown to be helpful adjuncts in the diagnosis and grading of human papillomavirus-associated cervical intraepithelial neoplasia. however, no clear guidelines are available regarding their use in routine practice. this study was designed to evaluate the efficacy of the 3 stains alone and in combinations to determine the most useful strategy in the diagnosis of cervical intraepithelial neoplasia and provide guidance i ...	2009	19269017
cervical cancer: physiology, risk factors, vaccination and treatment.	cervical cancer is the second most common female cancer after breast cancer. unlike many other cancers, however, it is largely preventable through screening and vaccination. therefore, it is necessary for nurses to educate women about cervical cancer, to have an awareness of the mortality rates associated with cervical cancer, and to promote the nhs cervical cancer screening programme. this article discusses the need for cervical screening and methods of prevention, identifies risk factors, trea ...	2009	19270604
human papillomavirus types by age in cervical cancer precursors: predominance of human papillomavirus 16 in young women.	in a population-based study conducted in guanacaste, costa rica, we investigated the human papillomavirus (hpv) types detected in 233 cases of cervical intraepithelial neoplasia (cin) 2/3 and cancer by age. cin2+ and cin3+ in young women were significantly more likely to be associated with hpv 16 than the same lesions in older women (80% of cin3+ were associated with hpv 16 among women ages 18-26 years compared with only 32% among women older than 55 years; ptrend=0.018). there were no differenc ...	2009	19273486
detection of oncogenic human papillomavirus and other predictors of anal high-grade dysplasia in men who have sex with men with abnormal cytology.	the incidence of anal high-grade dysplasia in men who have sex with men is increasing. anal cytology that shows atypical squamous cells of undetermined significance is common, nonspecific, and rarely predicts high-grade squamous intraepithelial lesion. we want to know whether hybrid-capture ii(r) testing for oncogenic human papillomavirus (human papillomavirus+) in men who have sex with men with atypical squamous cells of undetermined significance is beneficial and whether other predictors of hi ...	2009	19273953
mbl2 gene polymorphisms are correlated with high-risk human papillomavirus infection but not with human papillomavirus-related cervical cancer.	we investigated whether there is a correlation between mbl2 polymorphisms and the host susceptibility to human papillomavirus (hpv) infection and cancer development. toward this end, we analyzed mbl2 exon 1 polymorphisms in 172 women infected by strains of hpv that are associated with high-risk of cervical cancer development (or "high-risk" hpv infection), 105 of whom had hpv-related squamous cell carcinoma of the cervix (scc), as well as 105 women not infected by hpv. we demonstrated an associa ...	2009	19275921
epidermodysplasia verruciform-like lesions in an hiv patient.	epidermodysplasia verruciformis (ev) is a rare disorder involving widespread infection with specific human papillomavirus types and characteristic clinical lesions that may resemble verruca plana, tinea versicolor, psoriasis, or seborrheic keratoses. the most common hpv types found in ev are 5, 8, 17, and 20. histopathologically, lesions demonstrate stereotypical enlarged keratinocytes in the upper epidermis with gray-blue cytoplasm, enlarged round nuclei with pale chromatin, and one or multiple ...	2009	19281706
cidofovir induces an increase in levels of low-risk and high-risk hpv e6.	cidofovir is a nucleoside analogue that is used off-license to treat recurrent respiratory papillomatosis (rrp) caused by hpv6/11. however, the effect of this drug upon low-risk hpv 6/11 gene expression is unknown.	2009	19283851
vulvar cancer in young women: demographic features and outcome evaluation.	the objective of the study was to identify prognostic and environmental factors associated with vulvar carcinoma in young women.	2009	19286150
assessment of human papillomavirus and epstein-barr virus in lung adenocarcinoma.	the association of human papillomavirus (hpv) and epstein-barr virus (ebv) infection with non-small cell lung cancer is controversial. hpv and ebv prevalence in a uniform population of lung adenocarcinoma was investigated, hypothesizing that there would be differences seen between smokers and non-smokers and between sexes. patients involved in this study were selected from a single institution database of lung cancer. in total 497 patients with adenocarcinoma were identified and 110 patients had ...	2009	19287995
one in four california adolescent girls have had human papillomavirus vaccination.		2009	19288623
the evaluation of human papillomavirus genotyping in cervical liquid-based cytology specimens; using the roche linear array hpv genotyping assay.	to ascertain the usefulness of the roche linear array human papillomavirus (hpv) genotyping assay for assessing hpv genotypes in liquid-based cytology (lbc) samples and to evaluate this methodology within a cytopathology laboratory. these tests are of importance as persistent infection with high-risk hpv genotypes is considered a causal factor in the development of cervical cancer.	2009	19291176
human papillomavirus is detected in transitional cell carcinoma arising in renal transplant recipients.	we investigated the role of human papillomavirus (hpv) in the development of transitional cell carcinoma (tcc) arising in renal transplant recipients.	2009	19291536
dutch public health experts refute claims that human papillomavirus vaccination has health risks.		2009	19293220
endocervical adenocarcinomas with prominent endometrial or endomyometrial involvement simulating primary endometrial carcinomas: utility of hpv dna detection and immunohistochemical expression of p16 and hormone receptors to confirm the cervical origin of the corpus tumor.	determining the primary site of a uterine adenocarcinoma can be problematic in hysterectomy specimens due to the overlapping morphology of endocervical adenocarcinomas and endometrial carcinomas, particularly when both the corpus (usually lower uterine segment) and endocervix are involved and precursor lesions are lacking or difficult to distinguish from intramucosal spread of carcinoma from one site to the other. both preferential extension of endocervical adenocarcinomas into the endometrium ( ...	2009	19295407
five-year experience of human papillomavirus dna and papanicolaou test cotesting.	to estimate the 5-year age group-specific test positives for pap tests and human papillomavirus (hpv) testing in a large, general screening population of women 30 and older.	2009	19300322
central european vaccination advisory group (cevag) guidance statement on recommendations for the introduction of hpv vaccines.	vaccines against human papillomavirus (hpv), the primary causative agent in cervical cancer, are licensed. this paper contains the central european vaccination advisory group (cevag) guidance statement on the introduction of hpv vaccines in central europe. eight countries currently have medical representatives on cevag: the czech republic, estonia, hungary, lithuania, poland, romania, slovakia and turkey. by raising awareness and disseminating information, cevag aims to promote the efficient and ...	2009	19301033
genital and perianal herpes simplex simulating neoplasia in patients with aids.	genital and perianal herpetic ulcers are common in hiv-infected patients and chronic mucocutaneous ulcers persisting for more than 1 month are the hallmark of active aids status. however, atypical clinical manifestations of herpes simplex virus (hsv) may occur in immunocompromised patients presenting as tumor-like nodules or condylomatous or hypertrophic lesions, rather than a classic ulcer. such unusual presentations raise the risk of misdiagnosis and a delay in appropriate treatment. here we d ...	2009	19302018
occurrence of human papillomavirus in pterygia.	the aim of the study was to assess the occurrence of human papillomavirus (hpv) dna in pterygium.	2009	19302081
human papillomavirus type-distribution in vulvar and vaginal cancers and their associated precursors.	data on human papillomavirus (hpv) prevalence in vulvar and vaginal cancers are limited. these data are important to predict the potential future effect of prophylactic hpv vaccines. our aim was to conduct a systematic review of hpv type distribution in vulvar and vaginal invasive carcinomas, vulvar intraepithelial neoplasia (vin), and vaginal intraepithelial neoplasia.	2009	19305339
adolescent gynecology.	given new developments in the field of adolescent reproductive health, this review focuses on highlighting new guidelines and practice patterns in evaluation and management of adolescent gynecologic problems. first, understanding the proper techniques for the initial examination is key to establishing a long-term relationship with this age group. reservations about the first gynecologic examination are common, and the practitioner's goal is foremost to make the patient as comfortable as possible ...	2009	19305342
p21 cip1/waf1 expression is strongly associated with hpv-positive tonsillar carcinoma and a favorable prognosis.	human papillomavirus is involved in the carcinogenesis of tonsillar squamous cell carcinomas. here, we investigated the expression and the prognostic value of key cell cycle proteins in the prb and p53 pathways in both human papillomavirus type 16-positive and -negative tonsillar squamous cell carcinomas. using immunohistochemistry, 77 tonsillar squamous cell carcinomas with known human papillomavirus type 16 status and clinical outcome were analyzed for expression of ki67, p16(ink4a,) cyclin d1 ...	2009	19305381
[pathogenesis of human papillomavirus infection in patients with epidermodysplasia verruciformis].	epidermodysplasia verruciformis (ev), a rare inherited disease, is believed to be associated with human papillomavirus (hpv) infection. ever1/2 genes, dendritic cells, t lymphocytes, and the biological characteristics of hpv itself may play roles in the pathogenesis of hpv infection.	2009	19317070
primary cervical cancer truly negative for high-risk human papillomavirus is a rare but distinct entity that can affect virgins and young adolescents.	cancer of the uterine cervix is almost exclusively associated with human papillomavirus (hpv). carcinogenesis is slow, the minimal time from initial hpv infection to invasive carcinoma seems to be less than ten years. in order to identify rapid onset cervical cancer, we carried out a retrospective re-analysis of an extended cohort of patients with invasive cervical cancer, and reviewed cases identified within the cancer registry of lower saxony or using medline or isi data. no instances of a rap ...	2009	19317256
prevalence of chlamydia trachomatis and herpes simplex virus 2 in cervical carcinoma associated with human papillomavirus detected in paraffin-sectioned samples.	the aim of the study was to evaluate the frequency of occurrence of hpv and co-infection: chlamydia (c.) trachomatis and hsv-2 in cervical cancer.	2009	19317260
multiple-type human papillomavirus infection in male anogenital sites: prevalence and associated factors.	human papillomavirus (hpv) causes cervical cancer and is strongly associated with other anogenital cancers. multiple-type hpv infection has been associated with lengthier infection and precancerous lesions. little is known about multiple-type hpv prevalence and associated factors in men. we examined the prevalence of and risk factors for multiple-type hpv in primarily asymptomatic men. detection of 37 hpv types in male anogenital epithelium and semen was completed in 463 men in two u.s. cities. ...	2009	19318438
human papillomavirus genotype distributions: implications for vaccination and cancer screening in the united states.	limited data are available describing human papillomavirus (hpv) genotype distributions in cervical cancer in the united states. such studies are needed to predict how hpv vaccination and hpv-based screening will influence cervical cancer prevention.	2009	19318628
assessing the hpv vaccine: researchers confront complex interplay of factors.		2009	19318636
demographic and risk factors in patients with head and neck tumors.	the association between human papillomavirus (hpv) infection and the development of head and neck cancer has been documented recently. in this study on 86 head and neck cancer patients and 124 controls, data regarding demographics, behavioral risk factors, and risks related to hpv exposure were collected. hpv detection was carried out using polymerase chain reaction in the tumors and in oral exfoliated cells, and hpv typing by a reverse line blot assay specific for 37 hpv types. sera were tested ...	2009	19319944
[human papillomavirus vaccination in denmark].		2009	19321083
male circumcision for the prevention of hsv-2 and hpv infections and syphilis.	male circumcision significantly reduced the incidence of human immunodeficiency virus (hiv) infection among men in three clinical trials. we assessed the efficacy of male circumcision for the prevention of herpes simplex virus type 2 (hsv-2) and human papillomavirus (hpv) infections and syphilis in hiv-negative adolescent boys and men.	2009	19321868
[pseudoangiosarcomatous squamous cell carcinoma of the penis: a case report with clinicopathological and human papilloma virus analyses].	to further understand the clinicopathological, ultrastructural and molecular features of penile pseudoangiosarcomatous squamous cell carcinoma (pascc), and improve its diagnosis and treatment.	2009	19323373