Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins. | porcine leukocytes contained three homologous peptides, pg-1, 2 and 3, that manifested potent microbicidal activity against escherichia coli, listeria monocytogenes and candida albicans in vitro. the peptides ('protegrins') were composed of 16 (pg-2) or 18 amino acid residues, and, like tachyplesins (broad-spectrum antibiotic peptides of horseshoe crab hemocytes), they contained two intramolecular cystine disulfide bonds. considerably smaller than defensins, protegrins nevertheless showed substa ... | 1993 | 8335113 |
| immunization of mice with yersinia enterocolitica leads to the induction of antithyrotropin receptor antibodies. | it is well established that autoimmune graves' disease, which is characterized by hyperthyroidism, is mediated by autoantibodies to the thyrotropin receptor (tshr). although what initially triggers this autoantibody response is not known, a number of studies have suggested that yersinia enterocolitica, an enterobacteria, could initiate the immune response against the tshr. in this study, we produced antibodies against purified extracellular domain of human tshr (etshr) and showed that anti-etshr ... | 1993 | 8335920 |
| ifn-gamma inhibits the replication of listeria monocytogenes in hepatocytes. | we previously reported that the bulk of listeria monocytogenes injected intravenously into mice is taken up in the liver and replicates within the parenchymal cells (hepatocytes). although ifn-gamma is known to play an important role in host defenses to listerial infections of the liver, the mechanism(s) that underlies this role remains to be fully delineated. in the initial experiments presented here, we demonstrated the elevated expression of ifn-gamma message in the livers of mice during prim ... | 1993 | 8335936 |
| bacterial phospholipases c. | a variety of pathogenic bacteria produce phospholipases c, and since the discovery in 1944 that a bacterial toxin (clostridium perfringens alpha-toxin) possessed an enzymatic activity, there has been considerable interest in this class of proteins. initial speculation that all phospholipases c would have lethal properties has not been substantiated. most of the characterized enzymes fall into one of four groups of structurally related proteins: the zinc-metallophospholipases c, the sphingomyelin ... | 1993 | 8336671 |
| [weber syndrome caused by listeria abscess]. | a 63-year old diabetic man presented with left weber's syndrome and meningitic syndrome. csf examination showed moderate lymphocytic pleocytosis and elevated proteins with normal glucose content and sterile culture. blood cultures yielded listeria monocytogenes and the patient received ampicillin. while his neurological condition had partially improved, he died of heart failure. several mesencephalic abscesses were found at autopsy. | 1993 | 8337566 |
| variable binding affinities of listeriolysin o peptides for the h-2kd class i molecule. | previously we used the peptide-binding motif for the murine class i major histocompatibility complex molecule h-2kd to identify a nonamer peptide of the listeria monocytogenes listeriolysin (llo) protein that was recognized by cytotoxic t lymphocytes (ctl) in association with h-2kd. eleven nonamer peptides contained in the llo sequence were synthesized and one, llo 91-99, proved to be a ctl target. using peptide binding competition assays with h-2kd-restricted ctl, we show that 3 out of the 11 l ... | 1993 | 8344365 |
| the toxicologic interactions resulting from inhalation of carbon black and acrolein on pulmonary antibacterial and antiviral defenses. | the goal of this study was to investigate whether coexposures to carbon black and acrolein result in a toxicologic interaction having effects on lung defenses against infectious agents. this aim was accomplished through in vivo studies with inhalation challenges of infectious agents that probe the functional integrity of the multicomponent system that comprises the integrated defenses of the lungs. staphylococcus aureus was used for the alveolar macrophage (am) surveillance phagocytic system, pr ... | 1993 | 8346533 |
| distribution of listeria spp. in confectioners' pastries from western france: comparison of enrichment methods. | three hundred samples of pastry from 100 different suppliers in western france, including butter-cream, whipped dairy cream and custard filled cakes from each supplier, were collected and tested for the occurrence of listeria spp. in 25 g samples. listeria spp. were detected in 21.7% of he samples: listeria monocytogenes in 13.7%, listeria innocua in 10% and listeria seeligeri in 2.3%. thirteen samples were contaminated with two species simultaneously. the frequency of contaminated samples was n ... | 1993 | 8347428 |
| presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica in environmental samples taken from a supermarket delicatessen. | environmental swab samples collected on three occasions from a supermarket delicatessen were examined for the presence of listeria monocytogenes, motile aeromonads and yersinia enterocolitica. strains of y. enterocolitica belonging to a pathogenic biotype were not recovered, but isolates of l. monocytogenes and motile aeromonads were found. contaminated surfaces and items that came into direct contact with ready-to-eat food included a slicing machine, a knife and parsley that was to be used as a ... | 1993 | 8347431 |
| listeria monocytogenes--a model system for studying the pathomechanisms of an intracellular microorganism. | virulence of listeria monocytogenes is determined by a cluster of five genes in the order plca, hly, mpl, acta and plcb, which are coordinately regulated by a transcriptional activator, termed prfa. the gene for prfa is located in front of plca. mutations within each of these genes reduce the virulence considerably and render the mutants unable to properly multiply and/or spread within the infected host cells. under growth-limiting conditions prfa-dependent proteins are preferentially synthesise ... | 1993 | 8347937 |
| induction of murine peritoneal gamma/delta t cells and their role in resistance to bacterial infection. | previous studies have reported an association of gamma/delta t cells with microbial infection in both human lesions and murine infectious disease models. in this study we provide a comprehensive analysis of the conditions under which the induction of gamma/delta t cells occurs at a site of infection. we found a site-specific induction of gamma/delta t cells after the injection of listeria monocytogenes in the peritoneal cavity of c3h mice. no changes were seen in the splenic or lymph node popula ... | 1993 | 8350063 |
| [neurotropic properties of listeria monocytogenes and mycobacterium leprae in natural and experimental infections]. | 1993 | 8351384 | |
| ovine ophthalmitis associated with listeria monocytogenes. | 1993 | 8351784 | |
| inhibition of adhesion of enteroinvasive pathogens to human intestinal caco-2 cells by lactobacillus acidophilus strain lb decreases bacterial invasion. | salmonella typhimurium and enteropathogenic escherichia coli (epec) were found to adhere to the brush border of differentiated human intestinal epithelial caco-2 cells in culture, whereas yersinia pseudotuberculosis and listeria monocytogenes adhered to the periphery of undifferentiated caco-2 cells. all these enterovirulent strains invaded the caco-2 cells. using a heat-killed human lactobacillus acidophilus (strain lb) which strongly adheres both to undifferentiated and differentiated caco-2 c ... | 1993 | 8354463 |
| sodium chloride, potassium chloride, and virulence in listeria monocytogenes. | virulence, as determined in a mouse model, and the virulence factor activities of catalase, superoxide dismutase, and listeriolysin o were examined in a parental strain (10403s) and in a nonhemolytic mutant strain (dp-l224) of listeria monocytogenes. the cells were propagated in media containing various concentrations of sodium chloride or potassium chloride. strains 10403s and dp-l224 exhibited significant increases in catalase activity and listeriolysin o activity when grown in medium containi ... | 1993 | 8357245 |
| inhibitory effect against pathogenic and spoilage bacteria of pseudomonas strains isolated from spoiled and fresh fish. | the antibacterial effects of 209 pseudomonas strains isolated from spoiled iced fish and newly caught fish were assessed by screening target organisms in agar diffusion assays. one-third (67 strains) inhibited the growth of one or several of six target organisms (escherichia coli, shewanella putrefaciens, aeromonas sobria, pseudomonas fluorescens, listeria monocytogenes, and staphylococcus aureus), of which s. aureus and a. sobria were the most sensitive. the inhibitory action was most pronounce ... | 1993 | 8357253 |
| role of complement component c1q in phagocytosis of listeria monocytogenes by murine macrophage-like cell lines. | listeria monocytogenes is a facultative intracellular pathogen of a great variety of cells. among them, macrophages constitute the major effector cells of listerial immunity during the course of an infection. although the molecular bases of l. monocytogenes attachment and entry to phagocytes are not completely understood, it has been demonstrated that c3b significantly increases l. monocytogenes uptake by macrophages via complement receptor type 3. the first component of complement, c1q, is pres ... | 1993 | 8359889 |
| analysis of cytokine mrna expression in listeria-resistant c57bl/6 and listeria-susceptible a/j mice during listeria monocytogenes infection. | this laboratory previously reported that mrna expression for many cytokines, as determined by reverse transcription-polymerase chain reaction analysis, is induced rapidly in the spleen during murine listeriosis. in the present study, the patterns of cytokine mrna expression in spleens and livers of listeria-resistant c57bl/6 and listeria-susceptible a/j mice were compared. in addition, in situ hybridization was performed to evaluate the distributions of cytokine mrna-expressing cells in these ti ... | 1993 | 8359895 |
| intracytoplasmic growth and virulence of listeria monocytogenes auxotrophic mutants. | the intracellular growth of several auxotrophic mutants of listeria monocytogenes was examined in cell culture, and virulence was evaluated in mice by intravenous injection of log-phase bacteria. l. monocytogenes transposon insertion mutants requiring either uracil, phenylalanine, glycine, proline, or nicotinic acid for growth were fully virulent and grew similarly to the parental strain as shown by their growth rates in cell culture. those requiring all three aromatic amino acids (phenylalanine ... | 1993 | 8359896 |
| different roles of alpha beta and gamma delta t cells in immunity against an intracellular bacterial pathogen. | several bacterial pathogens of medical importance are able to persist and replicate inside host mononuclear phagocytes. protective immunity depends on specific t lymphocytes that induce granulomatous lesions at the sites of bacterial multiplication. listeria monocytogenes is an intracellular pathogen that replicates inside mononuclear phagocytes and hepatocytes of mice. invasion from the phagosomal compartment into the cytoplasmic compartment is the principal mechanism of intracellular survival. ... | 1993 | 8361537 |
| an animal model of foodborne listeria monocytogenes virulence: effect of alterations in local and systemic immunity on invasive infection. | development of foodborne listeriosis is dependent on an interplay between organism-specific virulence factors and host susceptibility. gastric inoculation of sprague-dawley rats was used as a model to explore listeria-specific virulence and host susceptibility. gastric inoculation leads to invasive infection with "smooth" hemolytic listeria monocytogenes but not with "rough" l. monocytogenes or other listeria species. infection is dose-dependent with an id50 of 10(6) virulent listeria monocytoge ... | 1993 | 8365049 |
| inhibition of enterobacteria and listeria growth by lactic, acetic and formic acids. | minimum inhibitory concentrations (mic) of undissociated lactic, acetic and formic acids were evaluated for 23 strains of enterobacteria and two of listeria monocytogenes. the evaluation was performed aerobically and anaerobically in a liquid test system at ph intervals of between 4.2 and 5.4. growth of the enterobacteria was inhibited at 2-11 mmol l-1, 0.5-14 mmol l-1 and 0.1-1.5 mmol l-1 of undissociated lactic, acetic and formic acids, respectively. the mic value was slightly lower with anaer ... | 1993 | 8365950 |
| a combined modified reverse dot-blot and nested pcr assay for the specific non-radioactive detection of listeria monocytogenes. | a modified reverse dot-blot assay was developed and used in combination with a nested pcr amplification of hly a for the specific detection of listeria monocytogenes. the deoxynucleotides and digoxygenin-11-dutp concentrations in the pcr were optimized for maximal sensitivity and economy. for the dot-blot hybridization, digoxygenin-labelled pcr products were directly spotted on nylon membrane-bound poly-dt tailed capture probes and the signal detection was either colorimetric or chemiluminescent ... | 1993 | 8366865 |
| inhibition of listeria monocytogenes by lactobacillus bavaricus mn in beef systems at refrigeration temperatures. | the ability of lactobacillus bavaricus, a meat isolate, to inhibit the growth of three listeria monocytogenes strains was examined in three beef systems: beef cubes, beef cubes in gravy, and beef cubes in gravy containing glucose. the beef was minimally heat treated, inoculated with l. bavaricus at 10(5) or 10(3) cfu/g and l. monocytogenes at 10(2) cfu/g, vacuum sealed, and stored at 4 or 10 degrees c. the meat samples were monitored for microbial growth, ph, and bacteriocin production. the path ... | 1993 | 8368843 |
| a ribosomal dna fragment of listeria monocytogenes and its use as a genus-specific probe in an aqueous-phase hybridization assay. | cdnas were prepared from the total rna of listeria monocytogenes atcc 19118 and used as probes to screen a genomic library of the same strain. four clones were identified which contained ribosomal dna fragments. recombinant dna from one of them was fractionated and differentially hybridized with the cdna probes to rna of l. monocytogenes and kurthia zopfii. the resulting hybridization pattern revealed an hpaii fragment of 0.8 kb that was specific for the l. monocytogenes strain. the nucleotide s ... | 1993 | 8368854 |
| monoclonal antibodies that react with live listeria spp. | seven monoclonal antibodies (mabs) against listeria spp. that were reactive with live listeria spp. were developed. two of these mabs (55-8 and 55-37) were members of the immunoglobulin m class, and all other mabs were members of the immunoglobulin g class. mab 55-23 reacted with 148 of 157 strains tested. mab 34-51 reacted with serotype 1/2a, 1/2b, and 1/2c strains and exhibited a scattered reaction pattern with strains belonging to other serotypes. mab 55-44 reacted with all of the strains bel ... | 1993 | 8368856 |
| detection of listeria monocytogenes with a nonisotopic polymerase chain reaction-coupled ligase chain reaction assay. | a polymerase chain reaction (pcr)-coupled ligase chain reaction (lcr) assay for the specific detection of listeria monocytogenes (m. wiedmann, j. czajka, f. barany, and c. a. batt, appl. environ. microbiol. 58:3443-3447, 1992) has been modified for detection of the lcr products with a nonisotopic readout. when a chemiluminescent or a colorimetric substrate for the nonisotopic detection of the lcr products was used, the pcr-coupled lcr gave a sensitivity of 10 cfu of l. monocytogenes. the detecti ... | 1993 | 8368859 |
| cellular motions and thermal fluctuations: the brownian ratchet. | we present here a model for how chemical reactions generate protrusive forces by rectifying brownian motion. this sort of energy transduction drives a number of intracellular processes, including filopodial protrusion, propulsion of the bacterium listeria, and protein translocation. | 1993 | 8369439 |
| characterization of a new class of tetracycline-resistance gene tet(s) in listeria monocytogenes bm4210. | the nucleotide sequence of the tetracycline (tc)-minocycline (mc)-resistance determinant of plasmid pip811 from listeria monocytogenes bm4210 has been determined. the gene, designated tet(s), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated m(r) of 72,912. the apparent 68-kda size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcript ... | 1993 | 8370538 |
| listeriosis of the placenta: clinicopathologic study of seven cases. | the purpose of our study was to evaluate the usefulness of placental examination in the diagnosis of prenatal listeriosis. | 1993 | 8372871 |
| growth response of listeria monocytogenes nctc 7973 in two different media at four incubation temperatures. | listeria monocytogenes nctc 7973 was cultivated in tryptose phosphate broth (tpb) and tryptone soya broth (tsb) at four different temperatures: 4 degrees, 20 degrees, 30 degrees and 37 degrees c. cells grown in tsb at 20 degrees c displayed maximum absorbance over cells grown in tpb at the same temperature. moreover, this observation was further confirmed by dry mass determination and viable count. we determined that cells grown in tsb at 20 degrees c exhibited significant enhanced growth as com ... | 1993 | 8373108 |
| effect of lps on epithelial integrity and bacterial uptake in the polarized human enterocyte-like cell line caco-2. | bacterial lipopolysaccharide (lps) has been speculated to facilitate bacterial translocation by a mechanism involving physical disruption of the gut mucosal barrier. polarized, cultured intestinal epithelial cells (caco-2 cells) were used to study the effect of lps on enterocyte structure, viability, and susceptibility to bacterial invasion. varying concentrations of biologically active lps were incubated with enterocytes for 1 and 16 hr. lps had no noticeable effect on enterocyte viability or m ... | 1993 | 8375030 |
| polysaccharides isolated from plant cell cultures of echinacea purpurea enhance the resistance of immunosuppressed mice against systemic infections with candida albicans and listeria monocytogenes. | polysaccharides (ep) isolated from large scale plant cell cultures of echinacea purpurea, have been shown to activate human and murine phagocytes. in this study we investigated the influence of ep on the nonspecific immunity in immunodeficient mice. ep was effective in activating peritoneal macrophages isolated from animals after administration of cyclophosphamide (cp) or cyclosporin a (csa). ep-treated macrophages exhibited increased production of tumor necrosis factor-alpha (tnf) and enhanced ... | 1993 | 8375943 |
| growth of listeria monocytogenes in camembert and other soft cheeses at refrigeration temperatures. | listeria monocytogenes survived and, under most conditions, multiplied when inoculated directly into the cheese milk of laboratory made camembert cheeses. the rate and extent of growth was reduced at lower storage temperatures. significantly higher rates of growth occurred at the surface compared with the centre of the cheeses, and these were probably associated with increased ph and proteolysis at the cheese surface due to the mould ripening process. similar results were obtained with camenbert ... | 1993 | 8376636 |
| macrophage colony-stimulating factor and the enhanced migration of monocytes are essential in primary but not secondary host defenses to listeria organisms. | nonimmune mice infected with listeria monocytogenes exhibited elevated expression of macrophage colony-stimulating factor (m-csf) mrna and the enhanced migration of mac-1 antigen-positive bone marrow-derived mononuclear phagocytes (bmmp) to their livers. treatment with monoclonal anti-m-csf antibody diminished the traffic of bmmp and promoted the replication of listeriae. immune animals infected with listeriae expressed significantly lower levels of m-csf mrna than did nonimmune animals. moreove ... | 1993 | 8376839 |
| listeria meningitis: identification of a cerebrospinal fluid inhibitor of macrophage listericidal function as interleukin 10. | the killing of bacteria gaining access to the central nervous system is insufficient and requires bactericidal antibiotics for treatment. the inefficient host response in cerebrospinal fluid (csf) is thought to be due to impaired phagocytosis in csf, and low local concentration of antibody and complement. in addition, the csf may contain inhibitors, disabling phagocytes to eliminate bacteria. we have assessed the bactericidal activity of macrophages in the presence of csf from mice infected intr ... | 1993 | 8376933 |
| mechanism of hepatic cytochrome p450 modulation during listeria monocytogenes infection in mice. | there have been numerous reports of altered drug clearance during episodes of viral infection and during the clinical use of recombinant interferons, but there have been very few reports regarding the effect of active bacterial infections on cytochrome p450-mediated metabolism. the objective of this study was to determine the mechanism by which the gram-positive bacteria listeria monocytogenes causes a depression of cytochrome p450-mediated biotransformation in mice. after induction with beta-na ... | 1993 | 8379989 |
| the zinc metalloprotease of listeria monocytogenes is required for maturation of phosphatidylcholine phospholipase c: direct evidence obtained by gene complementation. | the maturation of the 33-kda proenzyme to the 29-kda phosphatidylcholine phospholipase c (pc-plc) of listeria monocytogenes requires the production of the zinc metalloprotease encoded by mpl, the proximal gene of the lecithinase operon. we recently described a low-virulence lecithinase-deficient mutant of l. monocytogenes egd-smr, designated jl762, generated by a single insertion of transposon tn1545 in mpl. this mutant failed to produce the 29-kda pc-plc, an exoenzyme probably involved in cell- ... | 1993 | 8384163 |
| dual roles of plca in listeria monocytogenes pathogenesis. | the plca gene of listeria monocytogenes encodes a secreted phosphatidylinositol-specific phospholipase c (pl-plc). recent studies have established that transposon mutations within plca result in avirulence for mice and pleiotropic effects when examined in tissue-culture models of infection. genetic analysis reveals that many of the effects of the transposon insertions are due to loss of readthrough transcription from plca into the downstream gene prfa, which encodes an essential transcription fa ... | 1993 | 8388529 |
| regulation of the prfa transcriptional activator of listeria monocytogenes: multiple promoter elements contribute to intracellular growth and cell-to-cell spread. | the prfa gene product is a transcriptional activator of listeria monocytogenes determinants of pathogenicity. in this study, we provide direct evidence that the prfa protein is a site-specific dna-binding protein. additionally, we describe the characterization of two classes of l. monocytogenes mutants which contain transposon insertions either in the prfa structural gene (exemplified by strain dp-l1075) or within the prfa promoter region (exemplified by strain dp-l973). both mutants are complet ... | 1993 | 8388865 |
| splenic macrophage activation and functions in amyloid enhancing factor-induced secondary amyloidosis. study of phagocytosis, killing, respiratory burst, and mhc class ii surface expression. | secondary amyloidosis is a systemic disease characterized by the extracellular tissue deposition of insoluble fibrillar amyloid a protein. aberrant metabolism of serum amyloid a protein by reticuloendothelial cells is thought to result in the accumulation of fibrils within the tissue. treatment of mice with amyloid-enhancing factor (aef) in conjunction with an inflammatory stimulus (i.e., agno3) induced amyloid deposition within 48-72 h. the activation state of a macrophage largely defines its e ... | 1993 | 8391054 |
| mice lacking the tumour necrosis factor receptor 1 are resistant to tnf-mediated toxicity but highly susceptible to infection by listeria monocytogenes. | tumour necrosis factor (tnf), jointly referring to tnf alpha and tnf beta, is a central mediator of immune and inflammatory responses; its activities are mediated by two distinct receptors, tnfr1 (p55) and tnfr2 (p75) (reviewed in refs 1-3). the cytoplasmic domains of the tnfrs are unrelated, suggesting that they link to different intracellular signalling pathways. although most tnf responses have been assigned to one or the other of the tnf receptors (mostly tnfr1), there is no generally accept ... | 1993 | 8395024 |
| [comparison of 3 enrichment media for the study of listeria monocytogenes in foods]. | analysis organised by the bcr (bureau communautaire de référence) using material in form of capsules containing a strain of listeria monocytogenes of known concentrations in various types of foods were carried out within the interlaboratory trials. in this work, it is demonstrated that the leb (listeria enrichment broth) medium is, of the three media assayed, the most adequate to investigate this organism in foods. | 1993 | 8397968 |
| free versus liposome-encapsulated muramyl tripeptide phosphatidylethanolamide (mtppe) and interferon-y (ifn-y) in experimental infection with listeria monocytogenes. | the effect of free and liposome-encapsulated muramyl tripeptide phosphatidylethanolamide (mtppe) and interferon-y (ifn-y) on the resistance against listeria monocytogenes infection in mice was investigated. it was shown that administration of mtppe or ifn-y at 24 h before bacterial inoculation led to increased resistance against l. monocytogenes infection in terms of a decrease in bacterial numbers in liver and spleen. encapsulation of mtppe and ifn-y in liposomes increased their efficacy 33- or ... | 1993 | 8398570 |
| characterization of bacteriocins from enterococcus faecium with activity against listeria monocytogenes. | laboratory cultures and environmental isolates of bacteria were screened for antagonism towards listeria monocytogenes using an agar spot test. seven of the 163 strains that were tested, one streptococcus bovis, one enterococcus casseliflavus, two e. avium and three e. faecium, consistently displayed antilisterial activity. cell-free, ph-neutralized supernatants prepared from the three e. faecium strains (jbl1061, jbl1083 and jbl1351) exhibited strong antilisterial activity against l. monocytoge ... | 1993 | 8398626 |
| current microbiological considerations in food preservation. | changes in the composition and properties of certain foods, as well as development of food products to meet consumer demands relating to health, nutrition and convenience lead to concerns about the microbiological safety of such products. concerns also arise from emergence or recognition of the importance of certain microbial food pathogens or spoilage organisms, as well as from modifications in food processing, handling, storage and preparation procedures. these facts necessitate continuous ree ... | 1993 | 8398630 |
| increased risk of meningitis and bacteremia due to listeria monocytogenes in patients with human immunodeficiency virus infection. | the incidence, demographics, and clinical outcome of infections due to listeria monocytogenes in individuals infected with the human immunodeficiency virus (hiv) were evaluated by prospective population-based surveillance. during a 2-year study period, 37 cases of invasive listeriosis occurred in metropolitan atlanta (annual incidence, 0.8 case per 100,000 population). seven of these cases occurred in known hiv-infected individuals (19% of all cases); five had an aids-defining illness, and the o ... | 1993 | 8399870 |
| liver abscess due to listeria monocytogenes: case report and review. | involvement of the liver in cases of listeria monocytogenes infection is uncommon but has been manifested as solitary liver abscess, multiple liver abscesses, and hepatitis. we describe a 73-year-old diabetic woman who presented with a solitary liver abscess and prolonged fever, and we review the world literature on hepatic manifestations of l. monocytogenes infection. patients presenting with solitary liver abscesses uniformly recovered with antimicrobial therapy and abscess drainage, whereas a ... | 1993 | 8399880 |
| serological response in rabbits to listeria monocytogenes after oral or intragastric inoculation. | the serological response in rabbits against listeria monocytogenes after oral or intragastric inoculation was investigated. both the number of sero-positive animals and the average serum titres were higher in animals inoculated by the oral route. this difference was especially marked in rabbits inoculated with the lower dose (1 x 10(3) colony-forming units (cfu)), which developed a strong serological response (average serum titre of 1280 after 4 inoculations) in most of the inoculated animals (8 ... | 1993 | 8401424 |
| [a case of listeria rhombencephalitis with a secondary vasculitis suggested by mri]. | we reported a rare case of listeria rhombencephalitis with meningitis. a 48-year-old healthy man suddenly experienced high fever and headache, then he had lower cranial nerve's palsies and mental dysfunction developed during one week period. on admission, his temperature was 38 degrees c. he was slightly delirious and euphoric. he had nuchal rigidity, mild paresthesia over his left cheek to left upper lip, a right sixth nerve palsy, dysphagia, hiccup, nasal voice and left cerebellar ataxia. his ... | 1993 | 8403684 |
| inhibition of interferon-gamma by an interferon-gamma receptor immunoadhesin. | interferon-gamma (ifn-gamma) is an important cytokine which regulates inflammatory and immune response mechanisms. ifn-gamma enhances the presentation and recognition of antigens by inducing the expression of major histocompatibility complex (mhc) proteins, by activating effector t cells and mononuclear phagocytes, and by modulating immunoglobulin production and class selection in b cells. inappropriate production of ifn-gamma has been implicated in the pathogenesis of several autoimmune and inf ... | 1993 | 8406584 |
| epithelial cells secrete the chemokine interleukin-8 in response to bacterial entry. | bacterial invasion of mucosal surfaces results in a rapid influx of polymorphonuclear leukocytes. the chemotactic stimulus responsible for this response is not known. since epithelial cells are among the first cells entered by many enteric pathogens, we investigated the ability of epithelial cells to provide an early signal for the mucosal inflammatory response through the release of chemotactic cytokines. as shown herein, the chemokine interleukin-8 (il-8), a potent chemoattractant and activato ... | 1993 | 8406853 |
| antimicrobial activity of lactic acid bacteria isolated from sour doughs: purification and characterization of bavaricin a, a bacteriocin produced by lactobacillus bavaricus mi401. | three hundred and thirty-five lactic acid bacteria were isolated from sour doughs and screened for antagonistic activity. of these 145 showed activity against one or several of the indicator strains used in the screening. the antimicrobial activity of 18 isolates were due to a proteinaceous compound. these 18 isolates belonged to three different lactobacillus species: lactobacillus bavaricus, lactobacillus curvatus and lactobacillus plantarum. the spectrum of antimicrobial activity for the three ... | 1993 | 8407671 |
| bacteriocinogenic activity of lactobacilli from fermented sausages. | during the screening of the inhibitory activity of 254 strains of lactobacilli isolated from fermented sausages at different times of ripening, 22% of the strains showed inhibition that was not related to acid or hydrogen peroxide, towards one or more indicator strains. not all the strains were capable of secreting the inhibitory compound in the supernatant fluid. the characterization of the inhibitory compound from three strains showed that they were bacteriocins with a bactericidal mode of act ... | 1993 | 8407674 |
| polarized distribution of listeria monocytogenes surface protein acta at the site of directional actin assembly. | the facultative intracellular pathogen listeria monocytogenes can infect host tissues by using directional actin assembly to propel itself from one cell into another. the movement is generated by continuous actin assembly from one end of the bacterium into a tail, which is left behind in the cytoplasm. bacterial actin assembly requires expression of the bacterial gene acta. we have used immunocytochemistry to show that the acta gene product, acta, is distributed asymmetrically on the bacterial s ... | 1993 | 8408297 |
| acute bacterial meningitis in adults. a review of 493 episodes. | to characterize acute bacterial meningitis in adults, we reviewed the charts of all persons 16 years of age or older in whom acute bacterial meningitis was diagnosed at massachusetts general hospital from 1962 through 1988. we included patients who were admitted after initial treatment at other hospitals. | 1993 | 8416268 |
| listeria ivanovii is capable of cell-to-cell spread involving actin polymerization. | listeria ivanovii has been considered to be pathogenic to animals but has rarely been found associated with human infections. it has been claimed that l. ivanovii lacks the acta gene, which in l. monocytogenes encodes a protein required for interaction with host cell actin. using fluorescence microscopy and electron microscopy, we demonstrate that l. ivanovii can invade mammalian cells, lyse the phagosomal membrane, polymerize host cell actin, reorganize actin to form tails, and spread from cell ... | 1993 | 8418038 |
| distribution of pathogen inhibition in the lactobacillus isolates of a commercial probiotic consortium. | pure strains of lactobacillus ssp. isolated from a commercial probiotic consortium were checked in a double layer solid medium for their inhibition activities against selected pathogenic bacteria including serotypes of listeria monocytogenes, escherichia coli and salmonella. the antagonistic properties of the lactobacillus strains may be related to the production of bacteriocin-like compounds. all the pathogens tested were inhibited by one or a few strains of lactobacillus, the best inhibition w ... | 1993 | 8420917 |
| purification, partial characterization and plasmid-linkage of pediocin sj-1, a bacteriocin produced by pediococcus acidilactici. | pediococcus acidilactici sj-1, isolated from a naturally-fermented meat product, produced an antibacterial agent active against selected strains of lactobacillus spp., clostridium perfringens and listeria monocytogenes. the agent was bactericidal against sensitive indicators, and sensitive to proteolytic enzymes; it was identified as a bacteriocin, and was designated as pediocin sj-1. it was stable over a wide ph range (3-9), and apparently most stable in the lower part of that range. at ph 3.6, ... | 1993 | 8420920 |
| the effect of slurry storage and anaerobic digestion on survival of pathogenic bacteria. | the decline in viable numbers of salmonella typhimurium, yersinia enterocolitica and listeria monocytogene in beef cattle slurry is temperature-dependent; they decline more rapidly at 17 degrees c than at 4 degrees c. mesophilic anaerobic digestion caused an initial rapid decline in the viable numbers of escherichia coli, salm. typhimurium, y. enterocolitica and l. monocytogenes. this was followed by a period in which the viable numbers were not reduced by 90%. the t90 values of e. coli, salm. t ... | 1993 | 8420921 |
| comparative analysis of using mtt and xtt in colorimetric assays for quantitating bovine neutrophil bactericidal activity. | two different tetrazolium compounds were compared for use in a colorimetric assay for quantitating bovine neutrophil bactericidal activity against staphylococcus aureus, escherichia coli, listeria monocytogenes, and brucella abortus. the tetrazolium compounds tested included 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) and sodium 3,3'-[1[(phenylamino)carbonyl]-3,4- tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate (xtt). the mtt and xtt colorimetric bacteric ... | 1993 | 8423367 |
| lipopolysaccharide-induced selective priming effects on tumor necrosis factor alpha and nitric oxide production in mouse peritoneal macrophages. | preculture of thioglycollate-elicited c3heb/fej mouse peritoneal macrophages in vitro with subthreshold stimulatory concentrations of lipopolysaccharide (lps) can induce hyporesponsiveness (desensitization) to both tumor necrosis factor alpha (tnf-alpha) and nitric oxide (no) production when these cells are subsequently stimulated with 100 ng/ml of lps. we have established, however, that the primary dose of lps required for inducing downregulation of no production is significantly lower than tha ... | 1993 | 8426119 |
| optical dna-dna homology in the genus listeria. | twenty-three strains of the seven recognized listeria species were studied by using dna-dna optical hybridization. the level of error in the data was low. our results supported the results of rocourt et al. (j. rocourt, f. grimont, p. a. d. grimont, and h. p. r. seeliger, curr. microbiol. 7:383-388, 1982), although there was some overlap between listeria monocytogenes and listeria innocua. we suggest that there may be more than one cluster in the species l. monocytogenes or the species may form ... | 1993 | 8427807 |
| differential mechanisms of intracellular killing of mycobacterium avium and listeria monocytogenes by activated human and murine macrophages. the role of nitric oxide. | murine peritoneal macrophages activated with interferon-gamma (ifn-gamma) produce large quantities of nitric oxide and are efficient in the killing of certain intracellular pathogens. to examine the role of this mechanism in the killing of mycobacterium avium by murine and human macrophages, we infected mouse peritoneal macrophages and human monocyte-derived macrophages with m. avium and listeria monocytogenes and stimulated the cells with recombinant tumour necrosis factor (tnf), granulocyte-ma ... | 1993 | 8428392 |
| genetic characterisation of isolates of listeria monocytogenes from man, animals and food. | multilocus enzyme electrophoresis was used to assess genetic relationships between 95 isolates of listeria monocytogenes, most of which were isolated in australia and new zealand from man, animals and food. the isolates were separable into two major genetic divisions; the majority of those from human patients and animals were in division i, and the majority from those foods that were not specifically associated with human listeriosis were in division ii. isolates in division i were virulent, whe ... | 1993 | 8429537 |
| immunotoxic effects of the color additive caramel color iii: immune function studies in rats. | administration of the color additive caramel color iii (ac) may cause a reduction in total white blood cell counts in rats due to reduced lymphocyte counts. beside lymphopenia, several other effects in rat have been described. the effects are caused by the imidazole derivative 2-acetyl-4(5)-(1,2,3,4-tetrahydroxybutyl)imidazole (thi) and occur in rats fed a diet low in vitamin b6. in the present paper, immune function studies on ac and thi with rats fed a diet low, but not deficient in vitamin b6 ... | 1993 | 8432426 |
| listeria monocytogenes infection in beta 2 microglobulin-deficient mice. | beta 2 microglobulin-deficient mice, in which the beta 2m gene has been disrupted by homologous recombination, lack functional cd8 t cells and are able to contain but not resolve an intravenous immunizing inoculum of listeria monocytogenes. we present evidence that compensatory immunity in such immunodeficient mice was mediated by a population of gamma delta t-cell receptor-positive cells; in contrast, neither cd4 cells nor natural killer cells appeared to play any part in this process. these da ... | 1993 | 8432593 |
| murine peritoneal macrophages activated by the mycobacterial 65-kilodalton heat shock protein express enhanced microbicidal activity in vitro. | after an intraperitoneal (i.p.) injection of purified protein derivative, peritoneal macrophages from mice infected with mycobacterium bovis bacillus calmette-guérin (bcg) show an enhanced respiratory burst, inhibit the intracellular proliferation of toxoplasma gondii, and kill listeria monocytogenes more efficiently than peritoneal macrophages from normal mice. one of the immunodominant antigens of mycobacterium spp. is the 65-kda heat shock protein (hsp 65), and in the present study, we determ ... | 1993 | 8432607 |
| stimulating effect of deodan (an oral preparation from lactobacillus bulgaricus "lb51") on monocytes/macrophages and host resistance to experimental infections. | deodan is a lysozyme lysate from lactobacillus bulgaricus for oral administration which has shown antitumor activity in mice and humans. the effects of this preparation on some functions of monocytes/macrophages and on host resistance to experimental infections were examined. the oral administration to mice of deodan-150 mg/kg daily (the recommended dose in humans) caused an increase of the spreading ability and phagocytic activity of peritoneal macrophages, which showed morphological signs of c ... | 1993 | 8432622 |
| genetic basis of tetracycline resistance in food-borne isolates of listeria innocua. | eleven of 12 tetracycline-resistant listeria innocua strains, isolated from chicken or turkey frankfurters and mozzarella cheese, were shown to carry dna sequences which hybridized with the tet m probe; of these, two strains also hybridized with tet k. the remaining strain hybridized with the tet k probe only. the tet m determinant appeared to be located on the chromosome; in one case, it was transferable by conjugation to recipients listeria monocytogenes, listeria ivanovii, and enterococcus fa ... | 1993 | 8434927 |
| comparison of conventional and reversed phage typing procedures for identification of listeria spp. | of 225 listeria isolates evaluated, 199 had the same bacteriophage patterns by both the conventional (a. audurier, a.g. taylor, b. carbonelle, and j. mclaughlin, clin. invest. med. 7:229-232, 1984) and the new, easier to apply, "reversed" (m. j. loessner, appl. environ. microbiol. 57:882-884, 1991) phage typing procedures, 5 had different phage reactions, and the remaining 21 isolates were untypeable. thus, the overall typeability rate was 90.7%, and 97.6% of the typeable isolates had the same p ... | 1993 | 8434928 |
| identification and enumeration of listeria monocytogenes by nonradioactive dna probe colony hybridization. | a plasmid containing the cloned listeriolysin gene of listeria monocytogenes was used as a probe to identify listeria strains by dna colony hybridization. the probe dna was labeled with horseradish peroxidase in the presence of glutaraldehyde. after the hybridization and wash procedures, the hybrid molecules were detected by luminescence, which resulted from the oxidation of luminol by a horseradish peroxidase-hydrogen peroxide-coupled reaction. of the 150 listeria strains and 16 non-listeria st ... | 1993 | 8439145 |
| differentiation of listeria monocytogenes and listeria innocua by 16s rrna genes and intraspecies discrimination of listeria monocytogenes strains by random amplified polymorphic dna polymorphisms. | differences in the 16s rrna genes (16s rdna) which can be used to discriminate listeria monocytogenes from listeria innocua have been detected. the 16s rdna were amplified by polymerase chain reaction with a set of oligonucleotide primers which flank a 1.5-kb fragment. sequence differences were observed in the v2 region of the 16s rdna both between l. monocytogenes scott a and l. innocua and between different l. monocytogenes serotypes. although l. monocytogenes slcc2371 had the same v2 region s ... | 1993 | 8439157 |
| administration of dehydroepiandrosterone to burned mice preserves normal immunologic competence. | burned individuals display a reduced ability to elicit cellular and humoral immune responses and a depression in the vitro production of certain t-cell lymphokines. treatment of burned mice with 100 micrograms of dehydroepiandrosterone within 1 hour after injury resulted in preserving a completely normal capacity to produce t-cell-derived lymphokines and to generate cellular immune responses. in addition, dehydroepiandrosterone-treated thermally injured mice demonstrated an above-normal ability ... | 1993 | 8442690 |
| ro 23-9424, a new cephalosporin 3'-quinolone: in-vitro antimicrobial activity and tentative disc diffusion interpretive criteria. | the susceptibility of 410 clinical bacterial isolates to ro 23-9424, a novel cephalosporin 3'-quinolone, was determined. overall, 97% of enterobacteriaceae and 100% of staphylococci were inhibited by < or = 8.0 mg/l of ro 23-9424. only 60% of pseudomonas aeruginosa and 80-90% of pseudomonas spp. and xanthomonas maltophilia were inhibited by this concentration. enterococci and listeria monocytogenes were resistant to ro 23-9424. clinical isolates of moraxella catarrhalis, streptococcus spp., and ... | 1993 | 8444677 |
| fish oil and immune function. | 1993 | 8446307 | |
| ethanol ingestion increases susceptibility of mice to listeria monocytogenes. | excessive consumption of alcohol is associated with an increase in the frequency and severity of infectious diseases. ethanol adversely affects specific and nonspecific aspects of the immune response. we used a murine model to determine whether ethanol ingestion impairs host mechanisms of resistance to listeria monocytogenes. naive mice and mice immune to l. monocytogenes were pair-fed either a leiber-decarli liquid diet containing 7% (v/v) ethanol or an isocaloric control diet for 7 days. then, ... | 1993 | 8452211 |
| reduced ribosomal thermal denaturation in listeria monocytogenes following osmotic and heat shocks. | increased thermotolerance of listeria monocytogenes induced by exposure to a high nacl concentration or a sublethal heat shock was concurrent with increased thermal stability of the 30s ribosomal subunit as measured by differential scanning calorimetry. it is proposed that protection of the 30s subunit is a critical mechanism for increased thermotolerance. | 1993 | 8454183 |
| membrane damage and interleukin-1 production in murine macrophages exposed to listeriolysin o. | to obtain some insight into the interaction between listeriolysin o (llo) and the macrophage membrane, we examined the effect of purified listeria monocytogenes hemolysin on the viability and functions of mouse peritoneal exudate macrophages. the study showed that purified llo impaired a variety of functions of the macrophages. first, it suppressed the luminol-dependent chemiluminescence response of macrophages. second, it suppressed the phagocytic ingestion of opsonized sheep erythrocytes and l ... | 1993 | 8454336 |
| listeria monocytogenes endocarditis in a patient with a prosthetic aortic valve. | 1993 | 8454450 | |
| immune response in mice that lack the interferon-gamma receptor. | interferon-gamma (ifn-gamma) exerts pleiotropic effects, including antiviral activity, stimulation of macrophages and natural killer cells, and increased expression of major histocompatibility complex antigens. mice without the ifn-gamma receptor had no overt anomalies, and their immune system appeared to develop normally. however, mutant mice had a defective natural resistance, they had increased susceptibility to infection by listeria monocytogenes and vaccinia virus despite normal cytotoxic a ... | 1993 | 8456301 |
| outbreak of listeriosis in 1992. | 1993 | 8457433 | |
| a chimeric toxin to study the role of the 21 kda gtp binding protein rho in the control of actin microfilament assembly. | we have developed a new tool for studying the role of rho in actin stress fibre formation. clostridium botulinum exoenzyme c3 which affects actin microfilament assembly by adp-ribosylation of p21 rho was genetically fused in various ways to diphtheria toxin (dt). the resulting chimeric toxins were tested on vero cells. chimeras of c3 and both the a and b fragments of diphtheria toxin had reduced cell binding activities but were apparently able to penetrate into vero cells by the same mechanism a ... | 1993 | 8458345 |
| microbiological status of rabbit carcases in egypt. | a total of 40 new zealand white rabbits, 20 freshly slaughtered rabbits from an experimental farm and 20 processed rabbit carcases from grocery stores in beni-suef city, were examined bacteriologically. the mean values of aerobic plate counts at 37 degrees c and 1 degrees c, enterobacteriaceae counts, pseudomonas counts and staphylococcus counts of freshly slaughtered rabbits were 10(4) +/- 2 x 10(3), 8 x 10(2) +/- 10(2), 6 x 10(2) +/- 10(2), 3 x 10(2) +/- 10(2), and 10(2) +/- 60 organisms per g ... | 1993 | 8465608 |
| a model of food-borne listeria monocytogenes infection in the sprague-dawley rat using gastric inoculation: development and effect of gastric acidity on infective dose. | recent epidemiological evidence suggests that listeria monocytogenes (lm) is a food-borne pathogen in humans. a model of lm infection was developed using the sprague-dawley (sd) rat to study the interaction of lm with gastrointestinal epithelium as the first step in the pathogenesis of invasive listeriosis. conventionally raised, juvenile female sd rats were given 10(2)-10(9) virulent l. monocytogenes, serotype 4b or nonpathogenic listeria species. only rats given virulent lm developed dose-depe ... | 1993 | 8466809 |
| novel mechanism for the camp reaction between listeria monocytogenes and corynebacterium equi. | the synergistic hemolytic reaction between listeria monocytogenes, staphylococcus aureus and corynebacterium equi on blood agar plates was explored using a modified test-tube assay. a synergistic lysis of sheep red blood cells was observed with l. monocytogenes and either s. aureus or c. equi, using crude extracts. synergism was also found with l. monocytogenes and purified cholesterol oxidase from pseudomonas fluorescens and brevibacterium brevi. crude extracts of s. aureus sensitized sheep red ... | 1993 | 8466816 |
| separation of bacteria using agglutinins isolated from invertebrates. | the agglutination of a selection of gram-positive and gram-negative bacteria by the haemolymph and coelomic fluid from several invertebrates was studied. the haemolymph from lumbricus terrestris and limulus polyphemus caused the strongest agglutination of most of the bacteria studied. when the agglutinating fraction of lim. polyphemus was liganded to magnetic microspheres 53% of the cells in pure cultures of listeria monocytogenes c200, 15% of salmonella enteritidis 37782, 92% of staphylococcus ... | 1993 | 8468260 |
| [periprosthetic popliteal abscess caused by listeria monocytogenes]. | 1993 | 8469664 | |
| differences in the rate of intracellular killing of catalase-negative and catalase-positive listeria monocytogenes by normal and interferon-gamma-activated macrophages. | intracellular killing of catalase-positive bacteria by murine resident macrophages requires the presence of extracellular serum, whereas killing of catalase-negative bacteria can occur in the absence of serum. to find out whether the intracellular killing of bacteria by rifn-gamma-activated macrophages also requires serum stimulation, we investigated the handling of ingested catalase-negative and -positive listeria monocytogenes by peritoneal macrophages of normal swiss mice and mice injected i. ... | 1993 | 8469926 |
| mesenrhombencephalitis: mr findings in nine patients. | mesenrhombencephalitis is a serious form of brainstem inflammation predominantly involving the deep and vital portions of the brain, that is, the mesencephalon (midbrain) and rhombencephalon (pons, medulla). mesenrhombencephalitis is difficult to diagnose on the basis of clinical and laboratory findings alone, and access to this portion of the brain for surgical biopsy carries high morbidity. we describe the mr appearance of mesenrhombencephalitis and correlate the imaging findings with clinical ... | 1993 | 8470582 |
| a highly sensitive and fast nonradioactive method for detection of polymerase chain reaction products. | a novel system for the detection of polymerase chain reaction (pcr) products has been developed. the system is based on a pcr in which one of the primers is biotinylated and digoxigenin-11-dutp is incorporated during elongation. biotinylated pcr products are captured on streptavidin-coated solid supports, and alkaline phosphatase conjugated to anti-digoxigenin antibody is subsequently bound to the incorporated digoxigenin. the detection may be obtained with colorimetric, fluorescent, or luminesc ... | 1993 | 8470800 |
| ity influences the production of ifn-gamma by murine splenocytes stimulated in vitro with salmonella typhimurium. | the ity-lsh-bcg genetic locus in the mouse has been documented to confer innate resistance to at least three intracellular pathogens: salmonella typhimurium, leishmania donovani, and mycobacterium. expression of the resistance gene(s) results in a slower net growth of these pathogens in the reticuloendothelial system early postinfection. although it is clear that the resident macrophages in resistant mice are functionally superior with regard to antimicrobial activity, the exact mechanism(s) und ... | 1993 | 8473743 |
| cell-surface location of listeria-specific protein p60--detection of listeria cells by indirect immunofluorescence. | a specific polyclonal antiserum was prepared against a gel-purified 60 kda extracellular protein of listeria monocytogenes atcc 19111 corresponding to protein p60 previously detected in culture broths of l. monocytogenes strains mackaness and egd [kuhn, m. & goebel, w. (1989), infection and immunity 57, 55-61]. indirect immunogold labelling combined with transmission electron microscopy and high-resolution scanning electron microscopy were used to investigate the location and distribution of p60 ... | 1993 | 8473866 |
| listeria-associated pericarditis in an aids patient. | listeria monocytogenes is a pathogenic, facultative intracellular gram-positive rod, generally seen in cell-mediated immunocompromised states. in acquired immunodeficiency syndrome (aids), it most commonly presents as bacteremia or meningitis. an association with pericarditis has not been described previously in this group of patients. this article describes a case of pericarditis secondary to listeriosis involving a focal pancarditis and necrosis of the a-v node with subsequent refractory ventr ... | 1993 | 8474138 |
| immunity to intracellular bacteria. | intracellular bacteria are endowed with the capacity to survive and replicate inside mononuclear phagocytes (mp) and, sometimes, within certain other host cells. mp are potent effectors cells that are able to engulf and kill many bacterial invaders. therefore, intracellular bacteria had to exploit potent evasion mechanisms that allow their survival in this hostile environment. at the early phase, natural killer cells activate antibacterial defense mechanisms. during intracellular persistence, mi ... | 1993 | 8476559 |
| directional actin polymerization associated with spotted fever group rickettsia infection of vero cells. | members of the spotted fever group (sfg) of rickettsiae spread rapidly from cell to cell by an unknown mechanism(s). staining of rickettsia rickettsii-infected vero cells with rhodamine phalloidin demonstrated unique actin filaments associated with one pole of intracellular rickettsiae. f-actin tails greater than 70 microns in length were seen extending from rickettsiae. treatment of infected cells with chloramphenicol eliminated rickettsia-associated f-actin tails, suggesting that de novo prote ... | 1993 | 8478082 |
| listeria monocytogenes-induced gamma interferon secretion by intestinal intraepithelial gamma/delta t lymphocytes. | gamma/delta t cells represent a major proportion of intestinal intraepithelial lymphocytes (iel), and it has been suggested that these iel serve as a first immune barrier against microbial invasion and that they do so by destroying infected epithelial cells. in the present study, we confirm that both alpha/beta and gamma/delta iel from naive mice express potent cytotoxicity and produce gamma interferon (ifn-gamma) after t-cell receptor (tcr) engagement by specific monoclonal antibodies (mab). in ... | 1993 | 8478105 |
| characterization of an aromatic amino acid-dependent listeria monocytogenes mutant: attenuation, persistence, and ability to induce protective immunity in mice. | a transposon insertion mutant of listeria monocytogenes was shown to be deficient in prephenate dehydratase, an enzyme acting late in the pathway for biosynthesis of phenylalanine. this mutant had reduced virulence in mice. the mutant and parent strains persisted to the same extent in the tissues of infected mice and elicited similar degrees of splenomegaly. mice vaccinated with the mutant were protected significantly from subsequent challenge with virulent l. monocytogenes. | 1993 | 8478119 |
| identification of a phosphoenolpyruvate:fructose phosphotransferase system (fructose-1-phosphate forming) in listeria monocytogenes. | listeria monocytogenes is a gram-positive bacterium whose carbohydrate metabolic pathways are poorly understood. we provide evidence for an inducible phosphoenolpyruvate (pep):fructose phosphotransferase system (pts) in this pathogen. the system consists of enzyme i, hpr, and a fructose-specific enzyme ii complex which generates fructose-1-phosphate as the cytoplasmic product of the pts-catalyzed vectorial phosphorylation reaction. fructose-1-phosphate kinase then converts the product of the pts ... | 1993 | 8478337 |
| [listeria monocytogenes infection in hiv patients: 2 new cases]. | two new cases of infection due to listeria monocytogenes in two females with hiv are discussed, one of them with full blown aids and the other pregnant and without knowledge of her seropositivity until that moment. its clinical manifestation as a meningeal manifestation and bacteremia, coincide with the few cases described until now; different hypothesis invoked until now are reviewed to justify this infrequent association hiv-listeria. | 1993 | 8484037 |