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aetiology and epidemiology of acute gastro-enteritis in swedish children.in a prospective 1-year study, 144 children attending or admitted to hospital and 272 children outside hospital with acute gastro-enteritis and 200 controls were investigated by a broad panel of diagnostic methods for enteropathogenic agents in the faeces and for related antibody responses. enteropathogens were identified in 77% of the inpatients, 63% of the outpatients and 8% of the controls. rotavirus and yersinia enterocolitica were detected significantly more often among inpatients. altogeth ...19863734469
distribution of campylobacter jejuni in wild birds and serogroup of isolates by slide agglutination technique. 19863735880
the nucleotide sequences of two trna genes from campylobacter jejuni. 19863737410
isolation of campylobacter jejuni from intestinal contents of chickens in mexico city. 19863738283
[characteristics of the susceptibility to antimicrobial agents of campylobacter jejuni/coli strains]. 19863738362
campylobacter jejuni in hospitalized children with diarrhoea in chiang mai, thailand.in this hospital based study, campylobacter jejuni, although endemic in northern thailand was not found in a significantly higher percentage of diarrhoea than in control children. it was isolated from the stools of 14 of 208 diarrhoea (6.7%) and 6 of 108 (5.5%) control patients. ten of the 14 positive diarrhoea cases were mixed infections, other pathogens isolated simultaneously were enteropathogenic e. coli 4x, shigella spp. 3x, salmonella spp. 4x, rotavirus 3x, plesiomonas shigelloides 1x and ...19863738608
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
use of skirrow medium with membrane filters for selective isolation of campylobacter jejuni from stools. 19863743564
the prevalence of bacterial intestinal pathogens in a healthy rural population in southern india.in a one-year prospective survey bacterial intestinal pathogens unassociated with diarrhoeal episodes were isolated from 20.5% of stool samples from 48.5% of a stratified random sample of the population of a village in southern india. campylobacter jejuni was the pathogen most frequently isolated, followed by enteropathogenic serotypes of escherichia coli. the incidence of diarrhoea in the study population was lower than the frequency of isolation of bacterial intestinal pathogens. it is necessa ...19863746876
[rapid detection of anti-campylobacter jejuni antibodies by enzyme linked immunosorbent assay in the patients with acute enteritis. (ii)]. 19863747201
preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.short synthetic oligonucleotides have been covalently cross-linked to alkaline phosphatase using the homobifunctional reagent disuccinimidyl suberate. the oligomers, twenty-one to twenty-six bases in length, are complementary to unique sequences found in herpes simplex virus, hepatitis b virus, campylobacter jejuni and enterotoxigenic escherichia coli. each oligomer contains a single modified base with a 12-atom "linker arm" terminating in a reactive primary amine. cross-linking through this ami ...19863748805
campylobacter infection associated with raw milk. an outbreak of gastroenteritis due to campylobacter jejuni and thermotolerant campylobacter fetus subsp fetus.raw milk is identified with increasing numbers of outbreaks of gastroenteritis and is an important vehicle for transmission of campylobacter infection. unlike campylobacter jejuni, campylobacter fetus subsp fetus has not been associated with common-source outbreaks of gastroenteritis. this report describes an outbreak of gastroenteritis involving c jejuni and a thermotolerant strain of c fetus subsp fetus associated with raw milk. fifteen (39%) of 38 persons who attended a banquet in wisconsin i ...19863753617
strain characterization and grouping of campylobacter jejuni and campylobacter coli by interaction with lectins.strains of campylobacter jejuni and campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. heating of the campylobacter cultures to 100 degrees c and holding for 30 to 60 min greatly enhanced their reactivity with lectins and permitted the grouping of all but 3 of 155 cultures tested in this study without interference of autoagglutination and other nonspecific activities. the lectin reaction patterns of the heated cultures were stable and reproducible ...19863754264
[diseases caused by campylobacter jejuni in childhood]. 19863757003
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
chronic diarrhea associated with campylobacter jejuni infection in a cat.campylobacter jejuni was isolated from a cat with chronic diarrhea. the diarrheic cat and another cat (which previously had diarrhea) in the same household had bactericidal antibody titers to the c jejuni. clinical response to antibiotic therapy and not recovering campylobacter sp from normal feces after treatment also supported the diagnosis of campylobacter-associated diarrhea. although the owner had a protracted episode of diarrhea, c jejuni was not isolated from the owner's feces, nor was a ...19863759618
comparative efficacy of seven selective media for isolating campylobacter jejuni.diarrheal stools from 263 patients were inoculated on seven selective media: butzler selective medium, blaser medium, skirrow blood agar, preston campylobacter selective medium, preston campylobacter blood-free medium, butzler virion medium, and modified preston medium (with amphotericin b [2 mg/liter]). a similar number of campylobacter jejuni strains were isolated from all the media studied; nevertheless, the presence of competing fecal flora (ff) made the detection of suspect colonies difficu ...19863760137
[campylobacter jejuni enteritis in infants]. 19863764182
[acute campylobacter jejuni enterocolitis in children under 2 years of age]. 19863764191
[campylobacter jejuni septicemia]. 19863764855
viable but nonculturable stage of campylobacter jejuni and its role in survival in the natural aquatic environment.conditions influencing the survival of campylobacter jejuni in the natural aquatic environment have been determined. release of campylobacter spp. into natural waters by animal hosts is postulated to play a key role in the maintenance of viability and transmission of the organism in the environment. laboratory flask microcosms containing filter-sterilized stream water were used to test c. jejuni for the ability to remain viable in simulated natural systems. the microcosms were compared with the ...19863767358
[investigation on serotyping of the campylobacter jejuni group]. 19863769671
association with hela cells of campylobacter jejuni and campylobacter coli isolated from human feces.we developed a rapid in vitro test for determining the association of campylobacter jejuni and c. coli with hela cells. association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (ai). the reproducibility of the ai was checked by repeating the test six times, using four strains chosen at random. means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. the experimental conditions for which ...19863770943
techniques for the optimum recovery of cold injured campylobacter jejuni from milk or water.when broth was inoculated with cells of campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. it was greater at 43 degrees than 37 degrees c and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. a technique of pre-enrichment in non-selective culture broth at 37 degrees c for 2 h before the addition of antibiotics and incubation at 43 degree ...19863771411
modified selective medium for isolation of campylobacter spp. from feces: comparison with preston medium, a blood-free medium, and a filtration system.our previously described (h. goossens, m. de boeck, and j. p. butzler, eur. j. clin. microbiol. 2:389-393, 1983) selective medium, consisting of cefoperazone (15 mg/liter), rifampin (10 mg/liter), colistin (10,000 iu/liter), and amphotericin b (2 mg/liter) (medium m1), for the isolation of campylobacter jejuni and campylobacter coli from stool specimens was modified as follows: cefoperazone (30 mg/liter), rifampin (10 mg/liter), and amphotericin b (2 mg/liter) (medium m2). a comparative study of ...19863771769
biotype and serogroup distribution of campylobacter isolates from children in nigeria.a total of 101 campylobacter isolates from nigerian children with or without gastroenteritis were biotyped and serogrouped by using the lior typing schemes (h. lior, j. clin. microbiol. 20:636-640, 1984; h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). fifty-three (52.5%) of the isolates were campylobacter jejuni biotype i, 29 (28.7%) were c. jejuni biotype ii, 10 (9.9%) were campylobacter coli biotype i, and 9 (8.9%) were c. coli biotype i ...19863771772
rate of campylobacter spp. isolation in three regions of ontario, canada, from 1978 to 1985.isolation rates of campylobacter spp. were analyzed for an 8-year period, 1978 through 1985. three laboratories of the ontario ministry of health examined 146,842 human feces samples for bacterial pathogens, including campylobacter jejuni and campylobacter coli. campylobacter spp. were isolated from 5,580 specimens (3.8%), with monthly isolation rates ranging from 1.1 to 7.4%. the data showed a seasonal distribution of isolations, with peaks during the summer months (june to september). most inf ...19863771774
monosaccharide composition of lipopolysaccharides from campylobacter jejuni and campylobacter coli.the monosaccharide composition of the lps from 5 campylobacter jejuni strains and 7 campylobacter coli strains has been studied. all lps's contained kdo, heptose, glucosamine, glucose, and (with one exception) galactose. all c. jejuni and 3 c. coli lps's contained greater than 1% galactosamine. 3-amino-3.6-dideoxyglucose was present in all but one c. coli lps and in only one c. jejuni lps.19863772734
campylobacter bacteraemia: a report of 10 cases.ten cases of campylobacter bacteraemia, which were diagnosed over a three-year period, are described. four patients presented with acute gastrointestinal illness, three patients had acute febrile illnesses, two patients had cellulitis and one patient presented with ureteric colic. two patients had lifelong agammaglobulinaemia, two were undergoing corticosteroid therapy and there was one nosocomial infection. in contrast with previous reports, the most common species that was isolated was campylo ...19863773807
effect of ascorbic, isoascorbic and dehydroascorbic acids on the growth and survival of campylobacter jejuni.ascorbic acid (asa), added to nutrient broth at a concentration of 5 mmol/l, was bactericidal towards campylobacter jejuni grown at 42 degrees c in a micro-aerobic atmosphere. specific enzymes, radical scavengers, metal chelators and reducing agents were tested as possible antagonists to the cytotoxicity of asa. the addition of catalase or of the metal chelators ceruloplasmin or desferal did not prevent the cytotoxic effect of asa. the addition of the hydroxyl radical scavengers mannitol, format ...19863781941
faecal carriage rate of yersinia species.a total of 1,203 unselected routine faecal samples from 1,006 patients were cultured for yersinia species by a cold enrichment technique. seventy-five specimens (6.1%) from 63 patients were culture-positive for yersinia spp. fifty-two were yersinia enterocolitica, 22 yersinia frederiksenii and 1 yersinia intermedia. the predominant y. enterocolitica isolates belonged to biotype 1 - serotype 0:6, 30 or serotype 0:5, 27. y. frederiksenii strains were non-typable. forty isolates were recovered from ...19863782782
[modification of intestinal colonization and translocation of campylobacter jejuni by erythromycin and an extract of lactobacillus acidophilus in axenic mice].erythromycin (15 mg) or lyophylized heat-killed lactobacillus acidophilus (80 mg) were administered per os daily in germ-free mice infected at day o with 10(7)-10(8) campylobacter jejuni per os. bacterial colonization of intestine and bacterial translocation of c. jejuni toward mesenteric lymph nodes, blood and liver were then studied for 5 days. the results were compared with those from an infected untreated control group. compared to the control group, the numbers of free and mucosa-associated ...19863122638
heterozygosity at the km locus associated with humoral immunity to campylobacter jejuni.serum samples from 43 caucasian subjects convalescing from acute campylobacter jejuni infection were typed for nine gm and two km determinants. the sera were also used to measure iga, igg, and igm classes of antibody to acid-labile surface proteins of c. jejuni by an enzyme-linked immunosorbent assay. a highly significant association (p = 0.004) was found between km1/km3 heterozygotes and the level of iga antibodies. these results suggest the existence of complementary immune response genes whic ...19863274046
early treatment with erythromycin of campylobacter jejuni-associated dysentery in children.to evaluate the efficacy of early treatment with erythromycin on the duration of fecal excretion and of diarrhea associated with campylobacter jejuni, 170 patients, age 3 to 60 months, were randomly assigned in a double-blind fashion to receive either erythromycin ethyl succinate or placebo immediately after being seen at cayetano heredia hospital because of acute dysentery. the groups' pretreatment characteristics were comparable. of the 30 patients with stools positive for c. jejuni, 12 were i ...19863488385
susceptibility of clinical isolates of campylobacter jejuni to twenty-five antimicrobial agents.the in-vitro activity of 25 antimicrobial agents against 113 to 161 clinical isolates of campylobacter jejuni was tested by an agar dilution method. all strains were susceptible to the four aminoglycosides tested, to imipenem, chloramphenicol and norfloxacin. one strain (0.6%) was resistant to each of the following antibiotics: nalidixic acid, erythromycin and clindamycin. the frequency of tetracycline, metronidazole and cotrimoxazole resistance among our isolates was 14.5%, 56.4% and 96% respec ...19863493235
incidence of campylobacter spp. in broiler flocks monitored from hatching to slaughter.the incidence of campylobacter jejuni/coli was examined in five flocks of broilers monitored from hatch to slaughter, in feed and water and in litter samples. a total of 1440 samples from 720 broilers was examined. campylobacter spp. were not isolated from broiler chicks at 1 day of age and were only isolated from one broiler chick in one flock at 1 week of age. in three flocks campylobacter spp. were isolated from all chicks sampled at 4 weeks of age. in the fourth flock all chicks sampled were ...198618766507
egg penetration by campylobacter jejuni.hens eggs were immersed in suspensions of campylobacter jejuni (4 strains) and examined for penetration and infection. penetration was demonstrated using an egg moulding technique and by culture. c. jejuni was recovered from shell membranes, but not from the albumin or yolk. embryonic deaths were not significantly greater in test than control eggs. unlike a strain of salmonella virchow, which was used as a control, the c. jejuni strains did not survive for more than 6 hours after penetration. it ...198518766924
[isolation of campylobacter fetus subsp. jejuni from feces and transport media during a diarrhea epidemic]. 19853155666
in-vitro activity of pefloxacin compared to enoxacin, norfloxacin, gentamicin and new beta-lactams.the in-vitro activity of pefloxacin was compared with that of norfloxacin, enoxacin, nalidixic acid, gentamicin, cefotaxime, ceftazidime and, where appropriate, other beta-lactams against a total of 363 recent clinical isolates. an agar dilution procedure was used to determine mics and two inocula (10(4) and 10(6) cfu) were used throughout. pefloxacin inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-positive proteus spp., enterobacter spp., shigella ...19853156112
[digestive localizations of sexually transmitted diseases in male homosexuals].the common practice of sexual relations with many different and anonymous partners and the great variety of responsible micro-organisms account for the high incidence, growing complexity and uneasy prevention of sexually transmitted diseases of the digestive tract in male homosexuals. syphilis, gonorrhoea, papillomas, chancroid, donovanosis, herpes virus or chlamydia infections are known to be transmitted by anal coitus; amebiasis, giardiasis and shigellosis by oro-anal contact (faecal contamina ...19853160035
etiology of acute infectious diarrhea in a highly industrialized area of switzerland.during an 18-mo period between 1981 and 1982, a prospective study was conducted in 119 adult patients with acute diarrhea. a diarrhea-inducing microorganism or toxin could be identified in 38.7% of the patients. salmonella sp and campylobacter jejuni were the leading agents that caused diarrheal illness in 25% of the investigated population. clostridium difficile was found in 6%, mainly after previous antibiotic therapy. rotavirus was rarely isolated and enterotoxigenic escherichia coli were not ...19853964774
lipopolysaccharide characteristics of pathogenic campylobacters.most campylobacter jejuni strains are sensitive and most campylobacter fetus strains are resistant to the bactericidal activity in normal human serum. we purified lipopolysaccharides from campylobacter strains to determine whether their composition and structure relate to serum susceptibility. the lipopolysaccharide of two serum-sensitive strains was best isolated by the galanos procedure, but for two serum-resistant strains a cold-ethanol extraction was optimal. for each lipopolysaccharide prep ...19853967920
in vitro antimicrobial susceptibility, plasmid analysis, and serotyping of epidemic-associated campylobacter jejuni.campylobacter jejuni strains from 11 outbreaks were characterized by antimicrobial susceptibility, plasmid profile, and serotyping by the methods of lior et al. and penner and hennessy. all 31 strains were susceptible to erythromycin, clindamycin, chloramphenicol, kanamycin, tobramycin, streptomycin, and gentamicin. a total of 21 strains from nine outbreaks were resistant to one or more of the following antimicrobial agents: tetracycline, metronidazole, ampicillin, or carbenicillin. of the 31 st ...19853968203
susceptibility of campylobacter isolates to the bactericidal activity of human serum.although campylobacter jejuni and related thermophilic organisms are more common human pathogens than are campylobacter fetus, most bloodstream or systemic isolates are c. fetus. to understand the pathophysiology related to this observation, the authors studied susceptibility to the bactericidal activity of normal human serum of campylobacter coli, c. jejuni, and c. fetus isolates from feces and blood. in standardized assays, 10 of 15 c. jejuni and related isolates showed 90% kill (mean, 90.6% + ...19853968449
campylobacter jejuni-specific serum antibodies are elevated in healthy bangladeshi children.in bangladesh and other developing countries, isolation of campylobacter jejuni is common in healthy children, and the illness/infection ratio falls with age. to determine whether specific serum antibodies correlate with this phenomenon, using an enzyme-linked immunosorbent assay, we studied sera from 93 healthy bangladeshi children and 121 healthy u.s. children under 15 years of age. for each age group (less than 1, 2 to 4, and 5 to 14 years) studied, specific serum antibody levels were signifi ...19853972984
illness associated with campylobacter laridis, a newly recognized campylobacter species.campylobacter laridis, a recently described thermophilic campylobacter species found principally in seagulls, has not previously been linked to illness in humans. six clinical isolates of this species were referred to the national campylobacter reference laboratory in 1982 and 1983. each isolate was confirmed by biochemical characterization and by dna relatedness studies. the six isolates were obtained during an illness: enteritis in four, severe crampy abdominal pain in one, and terminal bacter ...19853972989
comparison of basal media for culturing campylobacter jejuni and campylobacter coli.four strains of campylobacter jejuni and four strains of campylobacter coli were used to compare the quantitative growth of campylobacter cells on blood agar base no. 2 (oxoid), brucella agar (bbl microbiology systems and difco laboratories), campylobacter agar base (difco), columbia blood agar base (difco and oxoid), and mueller-hinton agar (difco and oxoid). columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-f ...19853972990
the virulence of clinical and environmental isolates of campylobacter jejuni.the virulence of campylobacter jejuni and c. coli isolated from various water sources was compared with that of clinical strains by in vitro assays of adhesion, invasion and cytotoxicity to hela cells. variation in degree of attachment was observed, but this did not appear to be related to strain source, however, water strains were less invasive and less cytotoxic to hela cells than clinical strains as shown by immunofluorescence and electron microscopy. these differences were particularly evide ...19853973380
a one-year survey of campylobacter enteritis and other forms of bacterial diarrhoea in hong kong.the following enteropathogens were isolated from the faeces of 769 (10.2%) of 7,545 patients of whom 5,704 had diarrhoea or abdominal pain, attending a teaching hospital in hong kong during one year: salmonellae 458 (6.1%); vibrio parahaemolyticus 125 (1.7%); campylobacters 108 (1.4%); shigellae 83 (1.1%); others 19 (0.3%). further identification of the campylobacter isolates showed that 63 (58%) were campylobacter jejuni biotype 1, 44 (41%) were c. coli and only one was c. jejuni biotype 2. sev ...19853973381
appearance in patients' sera of antibodies against purified antigen of campylobacter jejuni and its relation to the bacterium-excreting period. 19853973420
the prevalence of campylobacter jejuni in random-source cats used in biomedical research. 19853973421
campylobacter jejuni mastitis in a cow: a zoonosis-related incident. 19853976151
prevalence of campylobacter jejuni and salmonella during pig slaughtering.it was found that 79% of healthy pigs, slaughtered in three different slaughterhouses in the netherlands, were intestinal carriers of campylobacter jejuni (mean number 4000 cfu per g), and 21% of the same pigs had salmonella in the intestinal tract (mean number 10 cfu per g). immediately after slaughter, campylobacter was swabbed from 9% of the carcasses and salmonella from 13%. it is concluded from these data that most of the contamination on carcasses does not originate directly from the intes ...19853976157
[isolation of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter swine depending on the season]. 19853976311
campylobacter jejuni-associated hemolytic-uremic syndrome: a possible role for endotoxin. 19853977526
intestinal parasites and bacteria in aboriginal children in south west australia.in a study of intestinal parasites in 697 aboriginal children under the age of 6 years in south-west australia, giardia lamblia was recorded in 26% and hymenolepis nana in 13.9%. g. lamblia infections occurred above 4 months of age, and h. nana infections above 18 months of age. there was a close correlation between infection with these two species (chi 2, p less than 0.001). campylobacter jejuni, the predominant bacterial species recorded, was isolated in 2.3%, and was related to bowel symptoms ...19853977790
use of auxotyping for epidemiological studies of campylobacter jejuni and campylobacter coli infections.a chemically defined medium developed for neisseria gonorrhoeae was modified to support the growth of campylobacter jejuni and campylobacter coli. a total of 76 isolates of c. jejuni and 14 isolates of c. coli were tested on this medium, which was designated campylobacter defined medium (cdm), over a 3-month period. although none of the c. coli isolates appeared to require amino acids, 51% of the c. jejuni tested required one and 7% required multiple amino acids for growth. an analysis of isolat ...19853988340
phagocytosis of campylobacter jejuni and its intracellular survival in mononuclear phagocytes.in vitro phagocytosis and intracellular survival of campylobacter jejuni strain 2964 in mononuclear phagocytes were studied. the following three types of mononuclear phagocytes were used: a j774g8 peritoneal macrophage line derived from balb/c mice, resident balb/c peritoneal macrophages, and human peripheral blood monocytes. when c. jejuni and mononuclear phagocytes were combined at a ratio of 75:1, light microscopy, fluorescent microscopy, and electron microscopy all indicated that c. jejuni c ...19853988342
evidence of udder excretion of campylobacter jejuni as the cause of milk-borne campylobacter outbreak.we describe a community outbreak of campylobacter enteritis associated with the consumption of untreated milk, apparently contaminated by two cows with campylobacter mastitis. the outbreak occurred in two phases. strains of campylobacter jejuni of the penner serogroup complex 4, 13, 16, 50 and preston biotype code 6100 were isolated from patients in both episodes and from the faeces of the cattle, milk filters, bulk milk and retail milk. milk samples from two of 40 milking cows were found to con ...19853989284
campylobacter jejuni isolated from a rectal biopsy specimen. 19853989330
fatalities associated with campylobacter jejuni infections.although campylobacter jejuni is now recognized as a common cause of gastroenteritis, fatalities associated with this infection in the united states have not been previously reported. two fatalities associated with c jejuni infections occurred over a two-year period in the denver metropolitan area. the first case was in a previously healthy 26-year-old woman who died following a two-day diarrheal illness. the second case was in a 69-year-old diabetic woman who died 19 hours after developing a ga ...19853989964
[spontaneous peritonitis caused by campylobacter jejuni in a cirrhotic patient]. 19853990397
[season-related incidence of campylobacter jejuni and campylobacter coli in fecal samples of healthy slaughter cattle]. 19853993275
estimation of campylobacter spp. in broth culture by bioluminescence assay of atp.the luciferin-luciferase bioluminescence reaction was used to estimate cell numbers of campylobacter jejuni and campylobacter coli in broth cultures based on a linear relationship between cell numbers (in excess of 10(4) to 10(5] and atp levels. the sensitivity was lower than that obtained with escherichia coli. the calculated amount of intracellular atp per cell of c. jejuni and c. coli ranged from 1.7 to 2.1 fg.19853994377
[epidemiologic studies of enteritis caused by campylobacter jejuni. iv. a study of the principal reservoir hosts]. 19853995570
typing of heat-stable and heat-labile antigens of campylobacter jejuni and campylobacter coli by coagglutination.a coagglutination system has been devised for typing heat-stable and heat-labile antigens of campylobacter jejuni and c. coli. the use of protein a-positive staphylococcus aureus cells carrying campylobacter sp. serotype antibody and the treatment of campylobacter sp. cells with dnase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. cells of s. aureus were sensitized with campylobacter sp. serotype antisera. four to fi ...19853998098
cadmium chloride susceptibility, a characteristic of campylobacter spp.we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ...19853998099
campylobacter infection in domestic dogs.campylobacter jejuni, a recently recognised human enteric pathogen, was recovered from the faeces of 21.7 per cent of domestic dogs with diarrhoea as compared with only 3.1 per cent of normal healthy dogs. the recovery rate from non-diarrhoeic, but unhealthy, domestic dogs was 6.7 per cent. the differences in the incidence of c jejuni between breeds was not statistically significant. the majority of isolations were from puppies between birth and six months old. the incidence of c jejuni in domes ...19854002595
campylobacter jejuni infections on college campuses: a national survey.we surveyed 303 colleges and universities to determine the frequency of isolations of campylobacter jejuni and other enteric bacterial pathogens. campylobacter cultures could be obtained at 74 per cent; 10 per cent of stool cultures yielded campylobacter, which was isolated 10 and 46 times more frequently than salmonella and shigella, respectively. the incidence of diagnosed campylobacter infections was 1.3 per 10,000 enrolled students per month. intercollegiate variation was not explained by di ...19854003634
serotyping of and hippurate hydrolysis by campylobacter jejuni isolates from human patients, poultry and pigs in the netherlands.three hundred strains of campylobacter jejuni, isolated from human patients, poultry and pigs were serotyped according to the penner-lauwers system and furthermore tested for hippurate hydrolysis. serotyping showed a close relationship between human and chicken strains, whereas there was little relationship between human and pig strains. a similar conclusion was drawn from the results of the hippurate hydrolysis test: 86% of human and 94% of poultry strains were positive, whereas 91% of pig stra ...19854004203
alterations of myoelectric activity associated with campylobacter jejuni and its cell-free filtrate in the small intestine of rabbits.we evaluated the effects of a culture of campylobacter jejuni and its cell-free filtrate on myoelectric activity of isolated ileal segments in new zealand white rabbits. hematoxylin and eosin staining and scanning electron microscopy were used to assess the association between histologic changes and alterations in intestinal myoelectric activity. a culture of c. jejuni was shown to cause a significant increase in repetitive bursts of action potentials (rbaps) (6.9 +/- 1.2 rbap/h; p less than 0.0 ...19854007424
acute polyneuritis with cranial nerve involvement following campylobacter jejuni infection. 19854009198
serotyping of campylobacter jejuni isolated from sporadic cases and outbreaks in northern norway.in the northern regions of norway, 123 cases of campylobacter jejuni gastroenteritis were either sporadic infections or due to five separate outbreaks during 1980-83. using a serotyping scheme based on thermostable antigens and the passive haemagglutination technique, 92 isolates were serotyped. excluding duplicates from the outbreaks, the serotype distribution based on 79 isolates showed that serotypes 6, 7 and 12, 40 were the most frequent in occurrence. each included 11 (13.9%) of the isolate ...19854013744
antigenicity of lipopolysaccharides from campylobacter jejuni and campylobacter coli in passive haemagglutination tests and enzyme-linked immunosorbent assays.passive haemagglutination tests and elisa were used to study the serological activity of homologous and heterologous rabbit antisera against lps prepared from various strains of c. jejuni/coli. in both test systems the homologous antisera exhibited serological activity against lps. the heterologous antisera showed some degree of intra- and inter- species cross-reactivity. the cross-reacting was most pronounced in the elisa. erythrocytes sensitized with untreated lps gave higher antibody-titres t ...19854013751
evaluation and diagnosis of acute infectious diarrhea.the appropriate approach to the diagnosis and management of acute infectious diarrhea is determined by the frequency and setting of the illness, the recognizable causes or syndromes, the cost and yield of available diagnostic tests, and the treatability of the disease. acute diarrhea affects everyone throughout the world from one to more than six times each year, depending on age, location, and living conditions. the range of identifiable viral, bacterial, and parasitic etiologies is great, and ...19854014291
laboratory infection of chicken eggs with campylobacter jejuni by using temperature or pressure differentials.fertile chicken eggs were infected in our laboratory with campylobacter jejuni suspensions by using temperature or pressure differential methods of inoculation. after 2 days of incubation, over 90% of the eggs carried c. jejuni when iron was present in the inoculum. this percentage declined rapidly until by day 8, less than 10% of the eggs were detectably infected. however, up to 11% of hatched, healthy chicks carried c. jejuni in their intestinal tracts. the isolated organisms were of the same ...19854015086
serotyping of campylobacter jejuni, campylobacter coli, and campylobacter laridis from domestic and wild animals.by using 50 unabsorbed antisera, we were able to serotype 272 (65.7%) of 414 thermotolerant campylobacters from wild and domestic animals, on the basis of heat-stable antigens identified by means of passive hemagglutination. forty-two serotypes were recognized. the pattern of serotypes detected in the various animal species was compared to human clinical isolates by using the czekanowski index (proportional similarity index). the highest degree of similarity to the clinical isolates was observed ...19854015088
gastroenteritis due to campylobacter jejuni in lagos, nigeria. 19854016913
development of a bacteriophage typing system for campylobacter jejuni and campylobacter coli.a bacteriophage typing system for campylobacter jejuni and campylobacter coli was developed with phages isolated from poultry feces. data for phage selection were generated from a set of isolates of c. jejuni and c. coli from humans in illinois. selection of 14 phages from the 47 phages available was assisted by determination of the sneath-jaccard similarity coefficients and subsequent unweighted pair-group arithmetic averaging cluster analysis. the typing set was reproducible and stable in the ...19854019737
biotypes and serotypes of thermophilic campylobacters isolated from cattle, sheep and pig offal and other red meats.in this study we examined 730 faecal samples of offal (mainly liver), mince-meat and sausage meat collected from abattoirs and retail butchers' shops for campylobacters. campylobacter jejuni or c. coli were isolated from 30.6, 10.5 and 6% of sheep, cattle and pig offal samples respectively. specimens collected from abattoirs were, in general, more often contaminated than material obtained from retail butchers' shops. only 1.4% of minced meats and sausage meats contained campylobacters. most isol ...19854020105
enrichment medium for isolation of campylobacter jejuni-campylobacter coli.a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...19854026287
transmission of campylobacter jejuni by the housefly (musca domestica).houseflies (musca domestica) were infected with campylobacter jejuni after being confined for 5 days in a horsfall isolator containing 25-day-old chickens known to be fecal excretors of the organism. contaminated flies, when subsequently transferred to a second unit, transmitted c. jejuni to specific-pathogen-free chickens. allowing a sample of 32 houseflies to ingest c. jejuni in a liquid suspension resulted in recovery rates of 20% from the feet and ventral surface of the body and 70% from the ...19854026732
role of litter in the transmission of campylobacter jejuni.autoclaved or non-autoclaved used broiler litter that was experimentally contaminated with campylobacter jejuni was capable of infecting specific-pathogen-free chicks maintained in modified horsfall isolators. artificially infected chicks became fecal shedders of c. jejuni, resulting in contamination of both autoclaved and non-autoclaved used broiler litter. fecal shedding of c. jejuni by litter-reared, artificially infected chicks persisted for at least 63 days after chicks were transferred to ...19854026733
community-wide surveillance of campylobacter jejuni infection. evaluation of a laboratory-based method.surveillance of infections due to campylobacter jejuni is needed to further define the epidemiology of this disease in the u.s. we assessed one potential method of community-wide surveillance, a laboratory-based reporting network. six microbiology laboratories that routinely culture fecal specimens for c. jejuni reported all isolates to seattle-king county health department for an 18-month period. further investigations on 476 cases reported from this broadly based network revealed that c. jejun ...19854028667
campylobacter colitis: histological immunohistochemical and ultrastructural findings.the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ...19854029720
biotyping schemes for campylobacter jejuni. 19854031047
biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam.a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ...19854031520
serum antibodies to heat-labile enterotoxin of campylobacter jejuni. 19854031551
a point-source outbreak of campylobacteriosis associated with consumption of raw milk.after a one-day field trip to a minnesota farm, 22 (45%) of 49 third-grade students and three (14%) of 21 adult chaperones developed campylobacteriosis. campylobacter jejuni was isolated from specimens of 13 children and one asymptomatic adult. illness was associated with the consumption of raw milk during a picnic lunch (odds ratio = 41.0, p less than .0001) and participation in hand milking of cows (odds ratio = 37.5, less than .0001). two additional findings implicated consumption of raw milk ...19854031557
experimental infection of hamsters with campylobacter jejuni.campylobacter jejuni is a major cause of diarrhea and enterocolitis in humans and animals. a mammalian model has been developed for studying pathogenesis of the disease in hamsters by colonizing the ileum and cecum with c. jejuni via either oral intubation after purgation or direct surgical inoculation into the cecum. this colonization resulted in production of disease including diarrhea and intestinal lesions that resembles enterocolitis due to c. jejuni in humans and domestic animals. the muco ...19854038721
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...19854044793
hypogammaglobulinemia and recurrent campylobacter jejuni infection. 19854045252
experimental infection of gnotobiotic mice with campylobacter jejuni: colonisation of intestine and spread to lymphoid and reticulo-endothelial organs.axenic and monoxenic c3h mice were used to develop an animal model for enteroinvasiveness and translocation of campylobacter jejuni. after oral administration of 10(7)-10(8) viable cells of c. jejuni on day 0 (d0), bacterial colonisation was followed quantitatively during 23 days by counting free luminal bacteria and tissue-associated bacteria in the duodenum, ileum and colon. the kinetics of bacterial colonisation were the same in axenic and monoxenic mice; bacteria were more numerous in distal ...19854045991
epidemiologic and laboratory investigation of an outbreak of campylobacter enteritis associated with raw milk.an outbreak of gastroenteritis occurred in march-april 1981, in wichita, kansas, and involved more than 250 persons who drank raw milk from a single local dairy. campylobacter jejuni was recovered from 60 of 116 (52%) persons in households that had one or more ill family members. a cohort study of families that belonged to a food cooperative that purchased raw milk from the implicated dairy showed a significant association between illness and having drunk raw milk. thirty-nine of 55 (71%) person ...19854050775
comparison of methods for isolating campylobacter jejuni from raw milk.the method of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) was compared with that of lovett et al. (appl. environ. microbiol. 46:459-462, 1983) for the ability to recover campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. the method of lovett et al. gave significantly greater recovery proportions.19854051491
serotyping of campylobacter jejuni from an outbreak of enteritis implicating chicken.an outbreak of campylobacter enteritis involving 7 of 17 people over a period of 5 days followed a dinner at a restaurant. a chicken casserole dish was implicated with a food-specific attack rate of 58%. campylobacter jejuni penner serotype 18/21/29, resistant to metronidazole, was isolated from 3 of 4 symptomatic patients and from three raw fresh chicken samples closely associated with the implicated chicken. numbers of c. jejuni in the chicken ranged from 5.3 x 10(1) to 7.5 x 10(2) colony form ...19854056451
motility as an intestinal colonization factor for campylobacter jejuni.the colonization of the intestinal tract of suckling mice by campylobacter jejuni was examined by orally challenging the mice with a wild-type strain and several nonmotile mutant strains which were isolated after treating the wild-type strain with mutagens. the wild-type strain had colonized the lower portion of the small intestine, the caecum and the colon 2 d after inoculation. two nonmotile strains, one of which (m8) had lost all the flagellar structure including the filament, the hook and th ...19854056739
chemically defined media for auxotyping of campylobacter jejuni.a set of chemically defined media has been developed for the cultivation of campylobacter jejuni strains of human origin. a minimal medium, a complete medium and 5 different nutrient-deficient media (ndm1-ndm5) are described. some of the strains investigated required l-methionine(lacking in ndm1), l-cystine and l-cysteine (ndm2), k2hpo4 (ndm 3), kh2po4 (ndm4) and nad, thiamine and calcium pantothenate (ndm5). 57.7% of the strains investigated required l-methionine. the strains grew at ph 6.6-7.7 ...19854060921
reversible expression of flagella in campylobacter jejuni.campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.19854066041
investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines.the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ...19854067286
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