Publications

plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant.	plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp.	1986	3005233
enteropathogens associated with acute diarrhea in hospitalized infants.	thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ...	1986	3007718
incidence of antibiotic resistance and characterization of plasmids in campylobacter jejuni strains isolated from clinical sources in alberta, canada.	antibiotic susceptibilities of 382 strains of campylobacter jejuni isolated in alberta, canada, in 1980 and 1981 were determined. although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. tetracycline resistance was noted in 6.8% of strains in 1980 and in 8.6% in 1981. moreover, resistance to high-level tetracycline (32-128 micrograms/ml) was always mediated by a plasmid of 45-50 kilobases. three transmissible ...	1986	3008970
pathogens that cause travelers' diarrhea in latin america and africa.	with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ...	1986	3014633
an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario.	fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ...	1986	3017528
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.	complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...	1986	3018039
comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia.	the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ...	1986	3020082
genetic studies of kanamycin resistance in campylobacter jejuni.	campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ...	1986	3021049
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter spp.	to determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...	1986	3079730
macrolide, lincosamide and streptogramin resistance in campylobacter jejuni/coli.	strains of erythromycin-resistant campylobacter jejuni/coli isolated in the united kingdom, canada and europe, were also resistant to tylosin, spiramycin and clindamycin and, like sensitive strains, were insensitive to virginiamycin and pristinamycin component b. these were designated as macrolide-lincosamide-streptogramin generalized resistant organisms and macrolide-lincosamide generalized resistant on the basis of patterns of resistance to pristinamycin components a and b. two erythromycin-se ...	1986	3084440
in vitro antibacterial activity of enoxacin (ci-919).	antibacterial activity of enoxacin was evaluated against more than 3,700 clinical isolates using the agar-dilution method and an inoculum of 10(4)-10(5) cells per site. for comparison other antibiotics appropriate for each species were also included. for most enterobacteria and for staphylococcus aureus, staphylococcus epidermidis and pseudomonas aeruginosa, the mic90 of enoxacin was below 2 mg/l. serratia marcescens was more resistant; the mic90 being 4 mg/ml. enoxacin also showed high activity ...	1986	3093961
[biotyping of campylobacter jejuni isolated from diarrhea patients].		1986	3095457
[prevalence of campylobacter jejuni complement fixing antibody in healthy people].		1986	3097197
[a food-borne infection caused by campylobacter jejuni serotype lauwers 19].	report on a outbreak due to contaminated food involving 556 schoolchildren who got their lunch from a school kitchen. the outbreak occurred abruptly without any secondary infectious. examinations for salmonellae, shigellae, enteritis-coli, yersiniae and rotaviruses were negative. however, campylobacter jejuni were isolated from 105 specimens of faeces in rostock. sixty of these strains and another 2 that had been isolated at the same time but had no relation to the outbreak in rostock, were furt ...	1986	3107256
anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections.	several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ...	1986	2421739
a comparative study of the rod and coccoid forms of campylobacter jejuni atcc 29428.	coccoid forms in cultures of a strain of the enteric pathogen campylobacter jejuni were investigated. a culture containing 100% coccoid forms was non-viable. coccoid forms had a lesser content of cytoplasmic components and nucleic acids than rods of c. jejuni. during the conversion to coccoid forms nucleotides leaked from the cells. the results of treatments with ionic and non-ionic detergents, and lysozyme and ethylenediaminetetraacetic acid indicated a changed cell wall in coccoid forms compar ...	1986	2422149
monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice.	eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ...	1986	2422256
isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli.	flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ...	1986	2424932
common and specific epitopes of campylobacter flagellin recognized by monoclonal antibodies.	murine monoclonal antibodies to campylobacter jejuni recognized a flagellin epitope common to most campylobacter species and an epitope restricted to c. jejuni and c. coli. these epitopes are distinct from the serotype-specific epitope recently detected on the flagellin and have not been described previously.	1986	2426201
location of epitopes on campylobacter jejuni flagella.	flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ...	1986	2430943
[campylobacter jejuni/coli in partridges (perdix perdix l.) and pheasants (phasianus colchicus l.)].		1986	3814066
[epidemiology and epizootiology of enteritis caused by campylobacter jejuni and campylobacter coli].		1986	3822876
[incidence of campylobacter jejuni/coli in swine and poultry. possible relationship to human disease on the basis of characteristics of species and serotype].		1986	3823733
[frequency of infection with campylobacter jejuni/coli and the occurrence of igg, igm and complement-fixing antibodies against the agent in workers in poultry abattoirs in santiago, chile].		1986	3825343
the viability of campylobacter jejuni on refrigerated chicken drumsticks.	radappertized chicken drumsticks were experimentally contaminated with suspensions of campylobacter jejuni in two trials. qualitative analysis on drumsticks with an initial level of contamination of 4.8 x 10(3) cfu/cm2 showed that viability was retained for at least 10 days of storage at either 9 degrees or -12 degrees c. in a second quantitative trial, the level of contamination declined from 9.9 x 10(2) cfu/cm2 to 4.5 x 10(1) cfu/cm2 after 7 days at -20 degrees c. thereafter, c. jejuni persist ...	1986	3798737
campylobacter jejuni contamination of eggs.	contamination of commercial table eggs with a fecal suspension containing 4.4 x 10(6) cfu/g campylobacter jejuni resulted in shell penetration in 3/70 eggs and recovery of the organism from homogenized egg contents in 1/70 eggs. viability of c. jejuni on the shell surface was retained for only 16 hours, attributed to desiccation of the fecal suspension. a field survey of three commercial laying farms and their associated egg-packing plants showed that hens demonstrated to be fecal shedders of c. ...	1986	3798738
detection of serum antibody response in patients infected with one strain of campylobacter jejuni with a dig-elisa method.	paired sera from 10 patients and a convalescence sample from one patient suffering from campylobacteriosis were analysed for igg, igm and iga antibodies against the infective organism (campylobacter jejuni pen 0:6,7) with a dig-elisa system. either formalinized, ethanol-inactivated or heat-inactivated preparations of the infecting organism were used as antigens. cross reactivity was tested with human sera having agglutinating antibodies against yersinia enterocolitica (n = 6) or salmonella typhi ...	1986	3808455
[septicemia caused by campylobacter jejuni].		1986	3810578
morphologic observations of experimental campylobacter jejuni infection in the hamster intestinal tract.	the authors have developed a model for the diarrhea and intestinal lesions seen in campylobacter jejuni enterocolitis by colonizing the hamster ileum and cecum with c jejuni. erythematous inflammation of the ileum and cecum and distention of the cecum with fluid were observed at autopsy. the cecal mucosa appeared edematous. epithelial abnormalities observed by light microscopy included focal edema, occasional hyperplasia, diffuse hyperemia, and infiltration of the lamina propria with leukocytes. ...	1986	3942198
immune response to campylobacter jejuni in a rural community in thailand.	we studied the prevalence of antibodies to campylobacter jejuni in serum from healthy thai villagers by using enzyme-linked immunosorbent assays with c. jejuni surface proteins as antigens. levels of c. jejuni-specific iga rose progressively through life, igg peaked in the second year of life and then fell, and igm peaked during late childhood and the teenage years. these findings confirm results observed in bangladeshi children, and they suggest there is intense early exposure and continued exp ...	1986	3944480
campylobacter jejuni diarrhea in a 3-day-old male neonate.		1986	3946343
multiple angiomatous proliferations of ileal stoma following campylobacter enteritis. effect of laser photocoagulation.	multiple polypoid sessile tumors arose at the tip of the ileal stoma of a 37-year-old man, who previously underwent proctocolectomy for ulcerative colitis. the lesions developed shortly after a well-established gastrointestinal infection with campylobacter jejuni with mucosal ulceration. the histological and ultrastructural examination showed angiomatous proliferations, reminiscent of pyogenic granuloma. the destruction of the tumors by laser photocoagulation was followed by superficial ulcerati ...	1986	3948633
distribution of sero-biotypes of campylobacter jejuni and c. coli isolated from paediatric patients.	during a one-year period, 258 isolates of campylobacter jejuni and c. coli were obtained from children with gastroenteritis or bacteraemia at the red cross children's hospital, cape town, south africa. these isolates were biotyped by hippurate hydrolysis, h2s production and tolerance to 2,3,5-triphenyltetrazolium chloride (ttc). our study indicated that 95.4% of the isolates were c. jejuni biotype 1, 1.5% were c. jejuni biotype 2 and 3.1% were c. coli; 70% of the isolates were resistant to ttc. ...	1986	3950960
a survey of campylobacter and other bacterial contaminants of pre-market chicken and retail poultry and meats, king county, washington.	as part of a larger study to determine the flow of campylobacter and salmonella from food animals to humans during 1982-83, 1,936 swabs were collected for bacteriologic study from pre-market chickens, retail poultry, and other retail meats as well as from equipment and work surfaces used to process such foods. of the 297 samples collected in a poultry processing plant, 56.6 per cent were positive for campylobacter jejuni/coli (cjc), as were 23.1 per cent of the 862 retail chicken, and 17.2 per c ...	1986	3953916
the role of poultry and meats in the etiology of campylobacter jejuni/coli enteritis.	to determine the role of meats as possible sources of infection leading to campylobacter jejuni/coli (cjc) enteritis, 218 cases and 526 controls were selected from the king county group health cooperative (ghc) population from april 1982 through september 1983. all subjects were interviewed regarding food consumption one week prior to case onset. consumption of chicken and cornish game hen were both associated with more than a doubling of the risk of cjc enteritis: for chicken (relative risk = 2 ...	1986	3953917
epidemic campylobacteriosis associated with a community water supply.	in may 1983, an estimated 865 cases of epidemic gastrointestinal disease occurred in greenville, florida. surveillance of pharmacy sales of antidiarrheal medicines suggested that the outbreak was confined to greenville and its immediate vicinity. surveys demonstrated that the gastrointestinal illness attack rates inside and outside the city limits were 56 per cent (72/128) and 9 per cent (7/77), respectively (relative risk (rr) = 6.2); consumption of city water was associated with illness (rr = ...	1986	3953920
epidemiologic study of campylobacteriosis in iowa cattle and the possible role of unpasteurized milk as a vehicle of infection.	bile samples were collected from 477 iowa dairy cows and were cultured for thermophilic campylobacters. the prevalence of thermophilic campylobacters in the bile was 15.5%. campylobacter jejuni and c coli from dairy cattle, chickens, pigs, sheep, and human beings were serotyped to develop host-species profiles. human and cattle serologic profiles were the most similar, and human and chicken profiles shared several similarities. epidemiologic data from 168 human cases of campylobacteriosis indica ...	1986	3954200
inactivation of campylobacter jejuni by chlorine and monochloramine.	campylobacter jejuni and closely related organisms are important bacterial causes of acute diarrheal illness in the united states. both endemic and epidemic infections have been associated with consuming untreated or improperly treated surface water. we compared susceptibility of three c. jejuni strains and escherichia coli atcc 11229 with standard procedures used to disinfect water. inactivation of bacterial preparations with 0.1 mg of chlorine and 1.0 mg of monochloramine per liter was determi ...	1986	3954344
methods for isolating campylobacter jejuni from low-turbidity water.	membrane filtration methods were developed and evaluated for the quantitative recovery of campylobacter jejuni from environmental waters of low turbidity. the best procedure studied involved passaging the test water through a filter (pore size, 0.45 micron) and plating it facedown on campylobacter-selective agar. the filter was removed after overnight incubation, and the plate was streaked for isolation and then reincubated. this method, with or without prefiltration through 5.0- and 0.6-micron- ...	1986	3954345
isolation of campylobacter jejuni from retail mushrooms.	campylobacter jejuni was isolated from 3 (1.5%) of 200 retail, polyvinyl chloride film-wrapped, fresh mushrooms. these results indicate that fresh mushrooms may indeed be a source of c. jejuni and support previously reported epidemiological data (seattle-king county department of public health, surveillance of the flow of salmonella and campylobacter in a community, 1984) which revealed an an elevated relative risk of developing campylobacter enteritis in individuals who consume mushrooms.	1986	3954354
campylobacter jejuni and infantile traveller's diarrhoea.		1986	3956542
evaluation of a blood-free, charcoal-based, selective medium for the isolation of campylobacter organisms from feces.	a blood-free, charcoal-based selective medium (csm) consisting of a columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32 mg/liter), vancomycin (20 mg/liter), and cycloheximide (100 mg/liter) supported the growth of campylobacter jejuni and c. coli with colony counts equivalent to those obtained on antibiotic-free horse blood agar. csm was compared to skirrow medium (skm) for the recovery of c. jejuni and c. coli from stools ...	1986	3958143
distribution and serotypes of campylobacter jejuni and campylobacter coli in enteric campylobacter strains isolated from children in the central african republic.	one hundred eighty-five enteric campylobacter strains isolated from diarrheic or healthy children in bangui (central african republic) were studied to determine their species and serotypes. c. coli was identified in 38.9% of all strains and in 43.9% of strains from diarrheic children. by the hemagglutination technique for heat-stable antigens, 73.5% of the strains could be serotyped. of the typeable strains, 75% were distributed among 13 more frequent serotypes. c. coli serotype pen 37,56 was th ...	1986	3958146
colonization of syrian hamsters with streptomycin resistant campylobacter jejuni.	a streptomycin resistant campylobacter jejuni inoculated per os into two populations of syrian hamsters (one endemically harboring c. jejuni, the other free of c. jejuni) established chronic colonization of the organism in both groups. diet, steroid administration, age of hamsters or prior exposure to c. jejuni did not appreciably alter incidence of diarrhea or colonization of c. jejuni. the majority of hamsters sampled during the course of the experiment (1 to 22 weeks) shed streptomycin resist ...	1986	3959531
[campylobacter jejuni. infections in northern norway in 1980-84].		1986	3961747
campylobacter jejuni septicemia--epidemiology, clinical features and outcome.	in 33 cases of campylobacter jejuni septicemia, the disease was more common at the extremes of age: infants made up a third of the reported cases while 24% of patients were older than 50 years. fever was noted in more than 80% of patients and chills in about a fourth. enteritis was present in 70% of cases, and the gastrointestinal tract was the principal source of septicemia. half of the patients did not have significant underlying disease but were at extremes of age, which may reflect relative ...	1986	3962297
[suitability of various culture media for medium-term storage of campylobacter jejuni/coli strains].		1986	3962367
the relative importance of various enteropathogens as a cause of diarrhoea in hospitalized children in chiang mai, thailand.	a study of 208 children aged under 15 hospitalized with diarrhea and 108 matched controls was conducted at chiang mai university hospital, chiang mai, thailand from may 1983 to april 1984 to investigate the incidence, seasonal distribution, and causative agents of diarrhea. 1 or more enteropathogens were isolated from 121 (58%) diarrhea patients and from 32 (30%) controls. in diarrhea patients, shigella spp. were most frequently isolated (22%) followed by enteropathogenic escherichia coli (epe ...	1986	3794260
low incidence of campylobacter enteritis in northern ireland.	in a prospective survey carried out over 9 months in 1984 in the department of bacteriology, belfast city hospital, campylobacter jejuni was isolated from 24 out of 1200 faecal specimens (2%) from patients with acute diarrhoea. this isolation rate is much lower than that from other parts of mainland britain which report an isolation rate of between 8 and 15%. it is difficult to explain this large discrepancy but the limited availability of unpasteurized milk and the generally worse summer here ( ...	1986	3794324
phagocytosis of campylobacter jejuni and c. coli by peritoneal macrophages.	guinea-pig resident peritoneal macrophages had no activity against freshly isolated campylobacter jejuni, whilst c. coli was phagocytosed and killed. the number of bacteria killed by macrophages always exceeded the number of those ingested, suggesting an extracellular mechanism of killing.	1986	3794651
occurrence of campylobacter jejuni in dog faeces from the streets of a southern chilean city.		1986	3602827
[studies on phage typing of campylobacter jejuni/coli].		1986	3577114
hepatic lesions in experimental campylobacter jejuni infection of mice.	mice orally infected with campylobacter jejuni developed focal infiltrative necrotic lesions in the liver, as determined by both histology and liver function tests. the initial histopathological feature was a focal infiltrative lesion in the parenchyma and portal triads. foci of infiltrative lesions became necrotic between days 30 and 60 post-inoculation (p.i.). during this period, portal infiltrates increased in severity. from month 4 p.i., focal areas of infiltrative necrosis in the liver pare ...	1986	3625168
toxic megacolon due to campylobacter colitis.	campylobacter jejuni is a common cause of acute infectious diarrhoea. most patients recover in less than a week, but 20 per cent may have a prolonged or severe illness [1]. we describe a patient who developed a fulminant toxic dilatation of the colon necessitating a subtotal colectomy.	1986	3598315
prevalence of campylobacter jejuni and campylobacter coli among wild and domestic animals in yamaguchi prefecture.		1986	3574158
methods for detecting and enumerating campylobacter jejuni and campylobacter coli in poultry.	methods, media, and biochemical tests for detecting, enumerating, isolating, and identifying campylobacter jejuni and c. coli in foods are summarized with special consideration of poultry and poultry products. information is drawn largely from the american public health association compendium of methods for the microbiological examination of foods and the us food and drug administration bacteriological analytical manual for foods. reference is also made to recently advanced techniques and proced ...	1986	3554203
isolation of campylobacter jejuni from diarrhoea patients in eastern province of saudi arabia.		1986	3571879
biotypes and serotypes among campylobacter jejuni strains in lagos.		1986	3568121
[comparison of campylobacter jejuni isolation methods and the effect of moisture content on colony morphology].	several isolation methods and media and the effect of moisture content on colony morphology were compared for the primary isolation of campylobacter jejuni. of the 200 rectal swabs from cattle and sheep tested for the isolation of c. jejuni, 19.5% were positive on butzler medium and 16.5% were positive on skirrow medium. the transportation of samples in modified cary-blair medium increased the isolation rate. of the 300 gallbladder from cattle and sheep tested for the isolation of c. jejuni, 5% ...	1986	3561277
[the incidence of campylobacter jejuni and other pathogenic bacteria in childhood summer diarrhea].	it was investigated the incidence of campylobacter jejuni which has been recognized as a common cause of akut gastroenteritis and other pathogenic bacteria in childhood summer diarrhea. it was examined 94 diarrheic patients stool in 0-5 years old in summer months 1985-86. campylobacter jejuni, salmonella, shigella were isolated as a pathogenic bacteria.	1986	3561278
extraintestinal campylobacter jejuni and campylobacter coli infections: host factors and strain characteristics.	to determine whether extraintestinal isolates of campylobacter jejuni and campylobacter coli are the consequence of unusual host or bacterial characteristics, we studied clinical and bacteriologic features of 24 extraintestinal infections. common serotypes and auxotypes were present among the extraintestinal isolates. gastrointestinal isolates were more susceptible to normal human serum than were the systemic isolates; however, the ranges overlapped considerably. predispositions to systemic spre ...	1986	3512731
western blot analysis of intestinal secretory immunoglobulin a response to campylobacter jejuni antigens in patients with naturally acquired campylobacter enteritis.	secretory immunoglobulin a (siga) response at the intestinal mucosa is a primary defense against enteric infections. we sought to determine which antigens of campylobacter jejuni outer membranes elicited siga responses in 8 patients with naturally acquired campylobacter enteritis using western blot analysis of fecal extracts. naturally acquired campylobacter infection elicited an siga response in 7 of 8 patients. of these 7 patients, 5 had campylobacter-specific siga titers of 1:16 and two had t ...	1986	3514359
two-dimensional thin-layer chromatography for the specific detection of hippurate hydrolysis by microorganisms.	glycine, one of the end products of hippurate hydrolysis by microorganisms, was detected by a rapid, specific technique utilizing two-dimensional thin-layer chromatography. a loopful of growth of each organism from its suitable agar medium was washed, suspended, and incubated with 0.1% sodium hippurate for 30 min at 37 degrees c. the supernatant of the incubated suspension from each organism was then dansylated, and the dansyl derivatives were separated by two-dimensional thin-layer chromatograp ...	1986	3517036
enrichment for detection of campylobacter jejuni.	gifu anaerobe-modified semisolid medium supplemented with vancomycin, polymyxin b, trimethoprim, and amphotericin b can serve as an efficient enrichment for the recovery of campylobacter jejuni under aerobic conditions. it is superior to the direct plating method in its recovery of very small numbers of c. jejuni. blood supplementation, agitation, and elevated temperatures are not required for this improved recovery.	1986	3519661
isolation of campylobacter jejuni in hunan, the people's republic of china: epidemiology and comparison of chinese and american methodology.	the incidence of infection with campylobacter jejuni was determined in individuals with and without diarrheal disease seen at a hospital in changsha, china. stool specimens were cultured by two methods: one developed by the chinese and a second control method used commonly in the united states. among people with diarrhea, 18.7% of the 48 children and 8.7% of 104 adults were infected with campylobacter jejuni. in the group without diarrhea, 8.6% of 105 children but none of 76 adults tested had th ...	1986	3522087
antigenicity of campylobacter jejuni flagella.	we studied the antigenicity of a wild-type flagellate and motile (f+m+) campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile (f+m-) and one aflagellate and immotile (f-m-). by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodalton (kda) band identified from sheared flagella as the flagellar protein was present in the f+m+ and f+m- strains but not in the f-m- strain. no other differences in protein profile ...	1986	3522430
control of shigella flexneri in celebes black macaques (macaca nigra).	stool specimens collected systematically from a group of celebes black macaques (macaca nigra) with a high incidence of diarrhea were examined microbiologically. numerous isolates of shigella flexneri, campylobacter jejuni and pathogenic escherichia coli were recovered. previous parasitology reports had revealed that the majority of the animals had balantidium coli. subsequently, the group was treated with trimethoprim-sulfamethoxazole, erythromycin and tetracycline. after treatment, shigella fl ...	1986	3523037
abortion and perinatal sepsis associated with campylobacter infection.	fetal loss or neonatal sepsis associated with campylobacter infection during pregnancy is infrequently recognized. as reported herein, one case of premature labor and neonatal sepsis due to campylobacter fetus subspecies fetus was treated successfully with ampicillin and gentamicin. only 19 similar cases have been cited in the literature. a review of these 19 cases reveals that the campylobacter species involved were probably c. fetus subspecies fetus in nine instances, campylobacter jejuni in n ...	1986	3523697
pathogenesis of campylobacter spp. in athymic and euthymic germfree mice.	adult athymic (nu/nu) and euthymic (+/nu) germfree balb/c mice were orally challenged with pure cultures of campylobacter jejuni (human clinical fecal strains) and a human blood isolate of campylobacter fetus subsp. fetus. after a period of adaptation to the mouse intestinal tract, all three c. jejuni strains caused disease in gnotobiotic mice. mouse-adapted, weakly cytotoxic c. jejuni 45100 consistently induced disease symptoms (transient diarrhea, cecal shrinkage, and acute inflammatory change ...	1986	3525411
probing of campylobacter jejuni with dna coding for escherichia coli heat-labile enterotoxin.		1986	3525701
[who discovered campylobacter jejuni/coli? a review of hitherto disregarded literature].	non culturable spiral form bacteria were noted in 1886 by theodor escherich. he found them in stool specimens and large intestinal mucous associated with diarrhoea in neonates as well as in kittens. in the following years till the end of the century a number of mainly german language publications appeared, describing the occurrence of such "spirilla" in cases of "cholera-like" and "dysenteric" disease. these organisms were found mainly in the colon or associated with mucous in diarrheal stool sp ...	1986	3526758
antibody response in patients infected with one common strain of campylobacter jejuni. evidence for production of antibodies against thermostable soluble antigens.	antibody production was investigated for ten patients from a group of 20 who had contracted infections with a strain of campylobacter jejuni of serotype pen 6,7. production of antibody was determined by titrating ten sets of paired sera for agglutination of formalin-treated and heat-treated cell suspensions and by passive hemagglutination using both sheep and human o rh-red blood cells sensitized with extracted soluble thermostable antigens. all patients had demonstrable antibodies against the f ...	1986	3528928
[experience in isolating campylobacter jejuni from patients with acute intestinal diseases].	erythrite agar, a commercial culture medium produced by the research institute for culture media (makhachkala, ussr), is suitable for the cultivation of c. jejuni after the addition of hemolized sheep blood. the addition of antibiotics to the medium permits its use for the isolation of c. jejuni from feces. by means of this medium c. jejuni has been isolated from the feces of 10% of patients hospitalized on account of acute infectious intestinal diseases.	1986	3529758
[epidemiologic evaluation of a sample of 91 rats (rattus norvegicus) captured in the sewers of lyon].	91 rats captured in 1982 in the sewers of lyon (france) have been examined for the presence of some microorganisms implicated in infections transmissible to man, by direct examination, bacteriological culture or detection of specific antibodies. bacteriological results have shown a high proportion of carriers of yersinia enterocolitica and frederiksenii (29%), of pasteurella pneumotropica (29%), of staphylococcus aureus (53%), and of campylobacter jejuni (18%), a low proportion of carriers of sa ...	1986	3532637
direct immunofluorescence microscopy for rapid screening of campylobacter enteritis.	diagnostic use of a direct fluorescent-antibody test for detection of campylobacter jejuni and c. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). conjugates were prepared from immunoglobulin g antibody against 22 lior c. jejuni and c. coli reference strains (h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). interestingly, the serotypes of cultures tested by the direct f ...	1986	3533990
[campylobacter jejuni/coli--isolated by coprocultures from cases of acute diarrhea].		1986	3538336
[incidence and transmission of campylobacter jejuni/coli in the production of young fattening poultry. 2. study of the characterization, resistance behavior and pathogenicity of campylobacter jejuni/coli in poultry].		1986	3542476
[comparison of various selective supplements in the cultivation of campylobacter jejuni/coli from fecal samples].		1986	3544537
survey of campylobacter jejuni and enterotoxigenic escherichia coli in kenya.		1986	3546042
the comparative activity of pefloxacin, enoxacin, ciprofloxacin and 13 other antimicrobial agents against enteropathogenic microorganisms.	in this study, we compared the activity of pefloxacin, enoxacin and ciprofloxacin against 269 enteropathogenic strains (campylobacter jejuni, enteropathogenic escherichia coli, salmonella typhi, shigella spp., vibrio cholerae and yersinia enterocolitica) with that of rosoxacin, flumequin, nifuroxazide, erythromycin, chloramphenicol, ampicillin, cefotaxime, tetracycline, amikacin, netilmicin, sulfamethoxazole, trimethoprim and co-trimoxazole. pefloxacin, enoxacin and ciprofloxacin were always amo ...	1986	3546145
approaches to the detection of enteric pathogens, including campylobacter, using nucleic acid hybridization.	a previous study of the detection of campylobacter jejuni in fecal samples spotted directly onto nitrocellulose filters before hybridization revealed a relatively low sensitivity and some false-positive results. we have investigated two factors that interfere with the detection of campylobacter jejuni in fecal samples: interfering substances that create false-positive background signals and nonspecificity of the probe. heterologous deoxyribonucleic acid probes bound nonspecifically to partially ...	1986	3698541
detection of pathogenic campylobacter species in blood culture systems.	because differences in recognition of campylobacter fetus and campylobacter jejuni in systemic infections may be due partially to differences in the ability to cultivate these organisms, we studied their growth characteristics in two widely used blood culture systems. in the roche septi-chek system (hoffman-la roche, inc., nutley, n.j.), over a broad range of inocula all strains were detected in broth within 2 days and on paddles within 3 days. in the bactec 6b aerobic bottles (johnston laborato ...	1986	3700626
the protection of infant mice from colonization with campylobacter jejuni by vaccination of the dams.	intraperitoneal vaccination of female mice, before mating, with a whole cell, heat-killed (62 degrees c) vaccine of campylobacter jejuni allowed the mother to confer immunity to her young, challenged orally 4-6 days after birth with the homologous strain. there was no protection against a strain of another serotype. heating the vaccine to 100 degrees c destroyed its protective properties. a vaccine prepared from an aflagellate variant of the original strain was as protective as the original vacc ...	1986	3701036
campylobacter jejuni in broilers: the role of vertical transmission.	the role of broiler eggs in the transmission of campylobacter jejuni to broiler grow-out flocks was investigated. six breeder flocks supplying broiler eggs to hatcheries were examined for cloacal carriage of c. jejuni. of 240 birds tested, 178 (74%) were c. jejuni-positive. eggs from these birds examined for c. jejuni penetration of the egg shell indicated that 185 of 187 were campylobacter-free. eggs from breeder flocks of unknown c. jejuni status were also examined for c. jejuni shell penetrat ...	1986	3701037
the cellular fatty acid composition of campylobacter species isolated from cases of enteritis in man and animals.	the cellular fatty acid composition of 41 strains of suspected campylobacter jejuni, 23 from human cases of gastroenteritis and 18 from animals, was examined by gas-liquid chromatography. three of the 23 human isolates and 2 of 18 animal isolates did not contain 19:0 cyclopropane fatty acid and were identified as c. laridis. the remaining 36 strains had cellular fatty acid profiles consistent with c. jejuni but could be divided into three groups on the ratio of the concentration of 18:1 and 19:0 ...	1986	3701040
pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni.		1986	3703054
ovine abortion associated with campylobacter jejuni.		1986	3705362
persistence of multiply antibiotic-resistant campylobacter jejuni in a patient with the acquired immune deficiency syndrome.	chronic diarrhea due to campylobacter jejuni has been described in patients with hypogammaglobulinemia. a patient with the acquired immune deficiency syndrome (aids) and normal total serum immunoglobulins had persistent diarrhea and c. jejuni on stool culture for seven and a half months despite repeated antibiotic therapy. antibiotic sensitivity studies revealed the c. jejuni to be multiply antibiotic-resistant. evaluation of the mechanism of resistance showed the organism harbored a conjugative ...	1986	3706381
susceptibility of campylobacter jejuni to strain-specific bactericidal activity in sera of infected patients.	campylobacter jejuni is a common cause of inflammatory enteritis, which in normal hosts is usually self-limited and resolves without antibiotic therapy. c. jejuni bacteremia is very rare. we examined sera for bactericidal activity that might be important in limiting the extent of c. jejuni infection in man. we studied the ability of nonimmune sera and homologous and heterologous immune sera from infected patients to kill different fresh case isolates of c. jejuni in vitro. the reduction of the l ...	1986	3710581
enzyme-linked immunosorbent assays for virulence properties of campylobacter jejuni clinical isolates.	to evaluate the capacity of enzyme-linked immunosorbent assays (elisas) to identify pathogenic strains among clinical fecal isolates of campylobacter jejuni, 40 consecutively obtained strains from 39 sick patients and 1 asymptomatic person were tested by respective elisas for enterotoxin production in culture filtrates and for the invasive virulence antigen of bacterial cells. of the 40 strains, 14 produced the enterotoxin; 15 strains, two of which were also enterotoxigenic, were invasive; and 1 ...	1986	3711295
[rapid detection of campylobacter jejuni by enzyme linked immunosorbent assay in the patients with acute enteritis (i)].		1986	3712788
effect of iron concentration on toxin production in campylobacter jejuni and campylobacter coli.	the effect of iron concentrations in culture media on supernatant yields of campylobacter cytotonic toxin (cct) was studied. of the 118 campylobacter spp. strains surveyed, 78.8% produced toxin in brucella broth or in casamino acids--yeast extract (cye) broth. when the iron concentration of cye was increased from 0.44 microgram/ml (7.9 microm) to 0.65 microgram/ml (11.6 microm) by the addition of ferric chloride, 94.9% of the strains were positive for toxin in a ganglioside gm1 based, enzyme-lin ...	1986	3719459
[serotyping of campylobacter jejuni/coli].		1986	3719684
campylobacter jejuni bacteraemia as a cause of recurrent fever in a patient with hypogammaglobulinaemia.	in a hypogammaglobulinaemic patient with faecal cultures persistently positive for campylobacter jejuni it was shown that c. jejuni bacteraemia was responsible for a long history of self-limiting attacks of fever. a focus of infection outside the gastro-intestinal tract was not found. antimicrobial treatment failed to eradicate c. jejuni. antibodies against c. jejuni were not detectable and there was also a defect in serum bactericidal activity. in contrast with normal serum, it was shown that, ...	1986	3722839
campylobacter jejuni appendicitis.		1986	3724634
effect of egg storage upon the survival of campylobacter jejuni in laboratory-infected fertile poultry eggs.	fertile eggs were infected by campylobacter jejuni in the laboratory by a temperature differential method of inoculation, which resulted in up to 10% of the hatched birds carrying c. jejuni in the intestine. when infected eggs were stored for 5 1/2 days before incubation, the infection rate of the eggs had decreased to 20% or less when set, and no infected chicks were hatched. inoculation of eggs after 8 days in storage also failed to yield infected chicks. in all cases, the hatch ratio was no d ...	1986	3729872
campylobacter jejuni associated with a perirectal abscess.		1986	3730961
colonization and infection of athymic and euthymic germfree mice by campylobacter jejuni and campylobacter fetus subsp. fetus.	human clinical strains of campylobacter jejuni and campylobacter fetus subsp. fetus colonized the gastrointestinal tracts of both athymic (nu/nu) and euthymic (+/nu) germfree mice (balb/c). viable campylobacter spp. (10(9) to 10(10) cfu/g [dry weight] of cecum and colon contents) were isolated on day 3 after oral challenge, and similar large numbers of viable cells were evident at several intervals during a 10-month experiment. the stomachs and upper small intestines of nu/nu and +/nu mice that ...	1986	3733222
monoclonal antibodies directed against the flagella of campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis.	nine monoclonal antibodies directed against the flagella of campylobacter jejuni strain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 penner serotype strains of c. jejuni. four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype of c. jejuni strain 81116. two monoclonal antibodies cross-reacted with all the flagellated strains of c. jejuni tested. one of these cross-reacting monoclonal antibodies, cf5, was fo ...	1986	3734423
natural campylobacter colonization in chickens raised under different environmental conditions.	a cross-sectional study of 447 laying hens (age range 0-65 weeks) and a longitudinal study of 164 similar birds showed that campylobacter jejuni was not present in the faeces of newly hatched chicks, but that colonization arose after 5-9 weeks. a survey of 250 broilers obtained from four breeders showed that all were negative for c. jejuni before and after slaughter at the age of 5 weeks. once c. jejuni had appeared in a flock, it rapidly spread to virtually all birds, but at the age of 42 weeks ...	1986	3734424