Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| the "dark side" of β-lactoglobulin: unedited structural features suggest unexpected functions. | the in-depth characterization of water buffalo (wb) whey proteins based on chromatographic and mass spectrometric techniques revealed unexpected structural co- and post-translational modifications for β-lactoglobulin (β-lg). the residues lys(47) and lys(69) of β-lg were found to be lactosylated early, at the time of milking. thiol groups of β-lg underwent a dynamic sulfhydryl/disulfide exchange that is probably essential in accomplishing specific physiological requirements in which proteins may ... | 2011 | 21507408 |
| methanogen diversity in the rumen of indian surti buffalo (bubalus bubalis), assessed by 16s rdna analysis. | the methanogenic communities in buffalo rumen were characterized using a culture-independent approach of a pooled sample of rumen fluid from three adult surti buffaloes. buffalo rumen is likely to include species of various methanogens, so 16s rdna sequences were amplified and cloned from the sample. a total of 171 clones were sequenced to examine 16s rdna sequence similarity. about 52.63% sequences (90 clones) had ⩾90% similarity, whereas, 46.78% of the sequences (81 clones) were 75-89% similar ... | 2011 | 21507441 |
| characterization of sarcocystis fusiformis based on sequencing and pcr-rflp in water buffalo (bubalus bubalis) in iran. | four sarcocystis species, i.e., sarcocystis fusiformis and sarcocystis buffalonis with cats as definitive hosts, sarcocystis levinei with dogs as definitive host, and sarcocystis dubeyi with unknown definitive host, have previously been described from water buffalo (bubalus bubalis). the aim of the present study was genetic characterization of the causative agent(s) of water buffalo sarcocystosis in khuzestan province, western iran. rflp-pcr and partial sequence analysis of 18s rdna gene were us ... | 2011 | 21526403 |
| characterization of {beta}-lactoglobulin from buffalo (bubalus bubalis) colostrum and its possible interaction with erythrocyte lipocalin-interacting-membrane-receptor (limr). | lipocalins form a widespread class of proteins involved in the transport of weakly soluble vitamins, hormones or hydrophobic molecules. β-lactoglobulin (blg-col), a major lipocalin present in whey was purified and characterized from buffalo colostrum. the molecular weight of blg-col as determined by lc-esi ms was 18.257 kda and the peptide mass fingerprint of the purified protein revealed 67% sequence homology to buffalo milk β-lactoglobulin. the n-terminal-iivtq and lc-esi-cid/etd ms/ms analyse ... | 2011 | 21546361 |
| production of cloned and transgenic embryos using buffalo (bubalus bubalis) embryonic stem cell-like cells isolated from in vitro fertilized and cloned blastocysts. | abstract here, we report the isolation and characterization of embryonic stem (es) cell-like cells from cloned blastocysts, generated using fibroblasts derived from an adult buffalo (baf). these nuclear transfer embryonic stem cell-like cells (nt-es) grew in well-defined and dome-shaped colonies. the expression pattern of pluripotency marker genes was similar in both nt-es and in vitro fertilization (ivf) embryo-derived embryonic stem cell-like cells (f-es). upon spontaneous differentiation via ... | 2011 | 21548826 |
| substitutes for endangered medicinal animal horns and shells exposed by antithrombotic and anticoagulation effects. | cornu saigae tataricae (antelope horn), manis squama (pangolin scale), cornu cervi pantotrichum (velvet antler) and cornu bovis grunniens (yak horn) are valuable medicinal animal horns and shells (mahs). as the major source of biological agents and ethnodrugs, mahs show pretty good bioactivities. however, with the increased demand for mahs, some of the medicinal resources are endangered, and there has been a concomitant increase in the prevalence of adulterated or impostor mahs. it is of great s ... | 2011 | 21549826 |
| effect of cytoplasmic volume on developmental competence of buffalo (bubalus bubalis) embryos produced through hand-made cloning. | abstract this study examined the effects of cytoplasmic volume on the developmental competence of hand-made cloned buffalo embryos. two different cell types, that is, buffalo fetal fibroblast (bff) and buffalo embryonic stem (es) cell-like cells were taken as donor cell and fused with one, two, or three demicytoplasts to generate embryos with decreased, normal (control), and increased cytoplasmic volume. using bff as a nuclear donor, the cleavage rate was similar in all the groups (p > 0.05), bu ... | 2011 | 21563942 |
| isolation of glucosidase and phospholipase positive bacillus circulans on aloa medium. | aim: to report the growth of glucosidase and phospholipase positive bacteria on agar listeria according to ottaviani and agosti (aloa) different from listeria monocytogenes, listeria ivanovii and bacillus cereus. methods and results: raw water-buffalo milk was analysed according to en iso 11290. streaking of fraser broth on aloa resulted in green colonies with an opaque halo after 48 h at 30°c. colonies were transferred onto tryptone soya yeast extract agar and showed cultural characteristics ... | 2011 | 21575023 |
| effect of low density lipoproteins in extender on freezability and fertility of buffalo (bubalus bubalis) bull semen. | this study was designed to determine whether low-density lipoporoteins (ldls) extracted from egg yolk in extender improve the freezability and fertility of buffalo bull semen. semen from three nili-ravi buffalo bulls was diluted at 37 °c with tris-citric acid extender (50 × 10(6) motile spermatozoa ml(-1)) containing ldls 2.5%, 5%, 10%, and 15% extracted from egg yolk and extender containing 20% egg yolk was kept as control. diluted semen was cooled to 4 °c in 2 h, equilibrated at 4 °c for 4 h, ... | 2011 | 21601914 |
| molecular characterization and prevalence of cystic echinococcosis in slaughtered water buffaloes in turkey. | the present study was carried out between march 2006 and june 2010. during the study nine abattoirs were visited and 166 water buffalo internal organs were examined in black sea region of turkey. it was found that 10.24% buffaloes were infected with cystic echinococcosis (ce). the rate of ce found as 3.77% in males and 21.66% in females, 37.93% in older and 4.38% in young animals. the degree of prevalence according to age and sex was statistically significant (p<0.05). ce was observed 29.41% onl ... | 2011 | 21601988 |
| rsai repetitive dna in buffalo bubalus bubalis representing retrotransposons, conserved in bovids, are part of the functional genes. | abstract: | 2011 | 21718551 |
| effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (bubalus bubalis) sperm. | this study investigated the apoptosis-like events associated with cryopreservation process and their relationship with cryocapacitation in buffalo (bubalus bubalis) sperm. a total of 49 semen ejaculates from seven bulls were studied for structural changes in sperm following cryopreservation. apoptotic changes were detected by assays specific for translocation of phosphatidylserine (ps) to the cell surface, alterations in membrane permeability and mitochondrial membrane potential (mmp), and dna i ... | 2011 | 21676035 |
| comparative evaluation of halothane anaesthesia in medetomidine-butorphanol and midazolam-butorphanol premedicated water buffaloes (bubalus bubalis). | six clinically healthy male water buffaloes (bubalus bubalis) 2-3 years of age and weighing 290-325 kg were used for 2 different treatments (h1 and h2). the animals of group h1 were premedicated with medetomidine (2.5 g/kg,i.v.) and butorphanol (0.05 mg/kg, i.v.), while in group h2 midazolam (0.25 mg/kg) and butorphanol (0.05 mg/kg) were used intravenously. induction of anaesthesia was achieved by 5% thiopental sodium in h1 (3.85 +/- 0.63 mg/kg) and h2 (6.96 +/- 0.45 mg/kg) groups. the anaesthes ... | 2011 | 21826832 |
| development and evaluation of real-time pcr assay for the detection of babesia orientalis in water buffalo (bubalus bubalis, linnaeus, 1758). | abstract babesia orientalis is the causative agent of babesiosis in water buffalo (bubalus babalis, linnaeus, 1758). in this study, a taqman real-time pcr assay was developed for quantitative detection of b. orientalis in water buffalo. hybridization probe and oligonucleotide primers were designed based on the v4 region of 18s rrna gene. detection limit was determined at 2 parasites. blood samples were collected from experimentally infected water buffalo, as well as from 180 field samples, which ... | 2011 | 21711103 |
| behaviour of listeria monocytogenes in packaged water buffalo mozzarella cheese. | aims:ôçé the aim of the study was to evaluate the behaviour of listeria monocytogenes in the conditioning liquid of packaged water buffalo mozzarella cheese (wbmc). methods and results:ôçé the conditioning liquid was contaminated with l.-ámonocytogenes, and the contaminated samples were stored at four different storage temperatures: 5 and 10-¦c for 22ôçâdays; 20-¦c for 9ôçâdays; 20-¦c for 3ôçâdays and then at 5-¦c for 6ôçâdays. the results showed that l.-ámonocytogenes concentration decreased wh ... | 2011 | 21740447 |
| changes in biochemical composition of follicular fluid during reproductive acyclicity in water buffalo (bubalus bubalis). | this study describes the changes in biochemical composition of follicular fluid during reproductive acyclicity in buffalo. a total of 73 pairs of ovaries collected from 26 reproductively acyclic and 47 reproductively cyclic buffaloes were used in the investigation. ovarian follicles were classified into small (5.0-6.9mm), medium (7.0-9.9mm) and large (ôëñ10.0mm) sized categories depending upon their diameter. follicular fluid was aspirated, processed and assayed for glucose, cholesterol, total p ... | 2011 | 21840653 |
| constant transmission of mitochondrial dna in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm. | although interspecies/intergeneric somatic cell nuclear transfer (iscnt) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iscnt embryos has been identified as an impediment to implementation for agricultural production. to investigate the nuclear-mitochondrial interactions on the developmental potential of iscnt embryos, we analyzed the mtdna copy numbers in iscnt embryos reconstructed with water buffalo (swamp type ... | 2011 | 21729201 |
| hormone variations in serum and milk of buffaloes (bubalus bubalis) as potential indicators of treatment with recombinant bovine somatotropin. | recombinant bovine somatotropin (rbst) is used to increase milk yield in cows, but it has been forbidden in some countries and in the eu. however, rbst misuse represents a concern in both bovine and buffalo dairy production. a number of studies on rbst treatment have been performed on bovines, but there are few data on buffaloes. in this study, we treated eight lactating buffaloes with biweekly injections of a slow-release formulation of rbst, for five cycles of administration, and analysed tota ... | 2011 | 21843393 |
| early pregnancy detection of iraqi riverine buffalo (bubalus bubalis) using the biopryn enzyme-linked immunosorbent assay for pspb and the progesterone assay. | this study was undertaken to detect pregnancy in iraqi riverine buffalo (bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy-specific protein b (pspb) with the biopryn(®) enzyme-linked immunosorbent assay (elisa) test. the aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. twenty-two female riverine buffalo that were 6.0 ± 0.93 years old were used. four ... | 2011 | 20825581 |
| activation of zona-free buffalo (bubalus bubalis) oocytes by chemical or electrical stimulation, and subsequent parthenogenetic embryo development. | parthenogenetic activation using zona-free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. the aim of this study was to compare the efficacy of different methods for the activation of zona-free buffalo oocytes in terms of their effects on the developmental competence of parthenogenetic embryos. the effects of zona removal on parthenogenetic activation and in vitro developmental competence of metaphase ii oocy ... | 2011 | 20825585 |
| the microanatomy of the palatine tonsils of the buffalo (bos bubalus). | the palatine tonsils play a key role in initiating immune responses against antigens entering the body through the mouth. they are also replication sites of some pathogens. there is no data available about the structure of the palatine tonsils of the egyptian water buffalo. therefore, palatine tonsils of 14 clinically healthy buffalo bulls (2-3 years old) were examined macroscopically and microscopically using light, and transmission electron microscopes. the tonsils had an elongated kidney shap ... | 2011 | 20833436 |
| freeze-thaw induced genotoxicity in buffalo (bubalus bubalis) spermatozoa in relation to total antioxidant status. | use of cryopreserved semen has become an important tool in assisted reproduction but freezing and thawing cause sub-lethal damage to spermatozoa. this is detrimental to sperm because of the membrane damage including permeability and integrity. an excess generation of reactive oxygen species (ros) creates oxidative stress due to reduced antioxidant status of the cryopreserved spermatozoa. in the present study fresh buffalo semen was collected and divided into two aliquots. one aliquot was used fo ... | 2011 | 20853151 |
| peptides from water buffalo cheese whey induced senescence cell death via ceramide secretion in human colon adenocarcinoma cell line. | milk proteins are a source of bioactive peptides. recent studies have indicated that protein-derived peptides released in buffalo cheese acid whey exert a cytomodulatory effect in human epithelial colon cancer (caco2) cells. the aim of the present study was to explain the molecular mechanism involved in the response of caco2 cells to oxidative stress in the presence of peptide fractions of buffalo cheese whey, purified and characterized by mass spectrometry. | 2011 | 20725925 |
| microvasculature of the buffalo (bubalus bubalis) choroid plexuses: structural, histochemical, and immunocytochemical study. | the choroid plexuses (cps) in mammals produce the cerebrospinal fluid (csf). in the literature, the morphology of cps and the process that regulates the production of csf are virtually nonexistent for domestic ruminants. thus this study has two aims: 1. to investigate the morpho-structure of the buffalo cp microvasculature utilizing light microscopy (lm), scanning electron microscopy (sem) and transmission electron microscopy (tem) techniques, and 2. to investigate the relationship between the b ... | 2011 | 21181712 |
| identification, characterization and expression analysis of hepcidin gene in sheep. | hepcidin is part of the innate immune system, and it plays a central role in the regulation of iron homeostasis. this peptide has been previously characterized in man, non-human primates, rat, mouse, dog, swine, cattle, horse, fishes, reptiles and birds but until now not in sheep. the aim of this study was to sequence, characterize and perform hepcidin expression analysis in different tissues collected from healthy sheep. the resulting open reading frame consisted of 249bp predicted to encode an ... | 2011 | 20723953 |
| green laser irradiation effects on buffalo semen. | the overall objective of this paper is to develop a more sensitive and less costly technique of laser irradiation of spermatozoa at certain wavelengths and exposure times suitable for improvement of buffalo semen quality. a 532 nm continuous wave (cw) dpss laser light has been used to irradiate buffalo semen for different time intervals. three semen pools from three different bulls (bubalus bubalis) were used in the experiment, each pool was divided into six groups : control (not irradiated), an ... | 2011 | 21220155 |
| standardisation of an indirect enzyme-linked immunosorbent assay for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis) in italy. | an indirect enzyme-linked immunosorbent assay (elisa) was evaluated for the detection of brucella antibodies in milk from water buffalo (bubalus bubalis linnaeus, 1758). the test accuracy was evaluated on milk samples from the campania region in italy. a total of 100 negative samples were collected from 10 officially brucellosis-free herds in salerno province, while 30 positive samples were collected from 3 herds in caserta province, where animals gave positive results to the official tests and ... | 2011 | 21240852 |
| effects of heavy metals and pesticides on buffalo (bubalus bubalis) spermatozoa functions in vitro. | industrial toxic metals, pollutants and bio-accumulative pesticides interfere with the male reproductive functions in farm animals. frozen-thawed semen samples were incubated with heavy metals (cadmium and lead) and pesticides (chlorpyrifos and endosulfan) of different concentrations (0, 0.005, 0.05, 0.02, 0.1, 0.5, 1.0, 2.0 and 4.0 μg/ml) for 1 h, and various spermatozoa functional parameters and in vitro fertilization rates were assessed. any significant effect was assessed by comparing the 1 ... | 2011 | 21241381 |
| free amino acids profile and quantities of 'sirt', 'bohca' and 'sekerpare' pastirma, dry cured meat products. | pastirma is a dry-cured and semi-fermented meat product, produced from whole beef or water buffalo muscles. sixteen to twenty different types of pastirma can be produced from a carcass, and each pastirma type has a different name, shape and quality characteristics. the objective of this study was to determine the free amino acid (faa) composition, ph and moisture values of 'sirt', 'bohca' and 'sekerpare' pastirma. | 2011 | 21254071 |
| analysis of active components of rhinoceros, water buffalo and yak horns using two-dimensional electrophoresis and ethnopharmacological evaluation. | cornu rhinoceri asiatici (rhinoceros horn, rh), cornu bubali (water buffalo horn, wbh), and cornu bovis grunniens (yak horn, yh) are traditional chinese medicine (tcm), and have been used in china for thousands of years. in this study, ethnopharmacological experiments were used to evaluate and verify the traditional efficacies of horns. area under curve (auc) was used to quantify the pharmacological efficacy strength of three horns. two-dimensional electrophoresis (2-de) was used to analyze the ... | 2011 | 21268260 |
| extended cytogenetic maps of sheep chromosome 1 and their cattle and river buffalo homoeologues: comparison with the oar1 rh map and human chromosomes 2, 3, 21 and 1q. | cytogenetic maps are useful tools for several applications, such as the physical anchoring of linkage and rh maps or genome sequence contigs to specific chromosome regions or the analysis of chromosome rearrangements. recently, a detailed rh map was reported in oar1. in the present study, we selected 38 markers equally distributed in this rh map for identification of ovine genomic dna clones within the ovine bac library chori-243 using the virtual sheep genome browser and performed fish mapping ... | 2011 | 21282943 |
| production of interspecies handmade cloned embryos by nuclear transfer of cattle, goat and rat fibroblasts to buffalo (bubalus bubalis) oocytes. | the possibility of producing interspecies handmade cloned (ihmc) embryos by nuclear transfer from donor cells of cattle, goat and rat using buffalo oocytes as recipient cytoplasts was explored. zona-free buffalo oocytes were enucleated by protrusion cone-guided bisection with a microblade. after electrofusion with somatic cells, reconstructed oocytes were activated by calcimycin a23187, treated with 6-dimethylaminopurine and were cultured in k-rvcl-50® medium for 8 days. although the cleavage ra ... | 2011 | 21288667 |
| long term effect of ovum pick-up in buffalo species. | the aim of this study was to evaluate the effect of an ovum pick-up (opu) treatment carried out for 9 months in buffalo (bubalus bubalis) species. eight pluriparous non-lactating buffalo cows underwent opu for 9 months. recovered cumulus enclosed oocytes (cocs) were classified and cocs suitable for in vitro embryo production (ivep) were in vitro matured (ivm), fertilized (ivf) and cultured (ivc) to the blastocyst (bl) stage. animals were monitored for a total period of 270 days, but at the summe ... | 2011 | 21306842 |
| dual promoter lentiviral vector generates transgenic mice expressing e2-csfv glycoprotein in their milk, but impairs early identification of transgenic embryos. | lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother. however, there are apparently no reports regarding early detection of transgenic embryos using a lentiviral vector carrying an additional transcription unit for tissue-specific expression of a valuable protein. in this study, two hiv-based lentiviral vectors were constructed. the first one contained the green fluorescent protein (gfp) co ... | 2011 | 21316751 |
| assessment of buffalo (bubalus bubalis) sperm dna fragmentation using a sperm chromatin dispersion test. | chromosomal fragmentations or damage in sperm dna has considerable value in determination of semen quality. however, rapid and/or simple method to assess sperm dna integrity in buffalo has apparently not been reported. in the present study, scd was used for the first time in buffalo bulls for assessment of sperm dna fragmentation. a modified scd protocol, under bright field microscope was developed and validated by comparison with other routine tests which can be used for processing of samples. ... | 2011 | 21382103 |
| biochemical and hormonal composition of follicular cysts in water buffalo (bubalus bubalis). | the objective of this study was to examine the follicular fluid biochemical and hormonal changes associated with ovarian follicular cysts in buffalo. follicular fluid was aspirated from eight cysts and eight preovulatory follicles, and subjected to biochemical and hormonal analyses. cysts were characterized by a greater (p<0.01) concentration of nitric oxide and lesser concentrations of ascorbic acid and glucose than that of preovulatory follicles (p<0.01 and p<0.05, respectively). furthermore, ... | 2011 | 21392901 |
| a rapid improved method for sexing embryo of water buffalo. | the objective of the experiment of this paper is to develop and improve in the sexing method for preimplantation embryos of water buffalo (bubalus bubalis) using loop-mediated isothermal amplification (lamp) reaction. embryo sexing has been recognized to control effectively the sex of offspring in the embryo transfer industry. a rapid and simple detection system was established by adding ethidium bromide (eb) or 5 ++l of cuso4 (3m) to the product of lamp reaction. the result of these additions a ... | 2011 | 21396688 |
| evaluation of mammalian and intermediate host surveillance methods for detecting schistosomiasis reemergence in southwest china. | schistosomiasis has reemerged in china, threatening schistosomiasis elimination efforts. surveillance methods that can identify locations where schistosomiasis has reemerged are needed to prevent the further spread of infections. | 2011 | 21408127 |
| improvement in the diagnosis of brucella abortus infections in naturally infected water buffaloes (bubalus bubalis) using an elisa with a protein-g-based indicator system. | brucellosis caused by brucella abortus in domestic water buffaloes (bubalus bubalis) raised under the traditional system of husbandry in northern india was diagnosed using an enzyme-linked immunosorbent assays (elisa) with a protein-g-based indicator system (protein-g elisa). a total of 1,551 animals that are positive (n = 61), negative (n = 243), and suspected (n = 1,247) for brucellosis were examined. rose bengal test (rbt) was used to predict the disease, and accordingly, animals were dichoto ... | 2011 | 21442153 |
| effect of straw size and thawing time on quality of cryopreserved buffalo (bubalus bubalis) semen. | this study was designed to compare the effect of straw size (0.25 vs. 0.5 ml) and thawing time (30 vs. 60 sec) on the quality of cryopreserved buffalo bull semen. sperm motility, plasma membrane integrity and viability were higher (p = 0.05) in 0.25 ml than 0.5 ml straw, thawed at 37°c either for 30 or 60 sec. in conclusion, cryopreservation of buffalo semen in 0.25 ml straw resulted in a higher post-thaw quality. | 2011 | 21455280 |
| fluorescence in situ hybridization mapping of six loci containing genes involved in the dioxin metabolism of domestic bovids. | six loci containing genes involved in the dioxin metabolism (arnt, ahr, cyp1a1, cyp1a2, cyp1b1 and ahrr) were assigned, for the first time, to cattle (bos taurus, 2n = 60, bta), river buffalo (bubalus bubalis, 2n = 50, bbu), sheep (ovis aries, 2n = 54, oar) and goat (capra hircus, 2n = 60, chi) chromosomes by comparative fish-mapping and r-banding using bovine bac-clones. the following chromosome locations were found: arnt to bta3q21, bbu6q21, oar1p21 and chi3q21, ahr to bta4q15, bbu8q15, oar4q1 ... | 2011 | 21465154 |
| brucellosis in domestic water buffalo (bubalus bubalis) of trinidad and tobago with comparative epidemiology to cattle. | the water buffalo is an important domestic animal worldwide, and the local buffalypso variety was developed in trinidad to have improved beef qualities. brucellosis was diagnosed in trinidad and tobago during 1998 in both cattle and domestic water buffalo (bubalus bubalis) populations. brucellosis in the latter species is caused by infection with brucella abortus, similar to bovine brucellosis. control of brucellosis is of paramount importance to preservation of the genetic diversity of these an ... | 2011 | 21479842 |
| effects of various plant protein sources in high-quality feed block on feed intake, rumen fermentation, and microbial population in swamp buffalo. | this study was designed to determine effect of various plant protein sources in high-quality feed block (hqfb) on feed intake, rumen fermentation, and microbial population in swamp buffalo. four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly assigned according to a 4 × 4 latin square design. four kinds of plant protein sources (coarse rice bran (crb), cassava hay (ch), phaseolus calcaratus hay, and mulberry hay (mh)) were mixed in the hqfb. hqfbs were allowed to be licked at fr ... | 2011 | 21479843 |
| pregnancy rates following ai with sexed semen in mediterranean italian buffalo heifers (bubalus bubalis). | the use of sexed semen in farm animal production and genetic improvement has been shown to be feasible with variable degree of efficiency in a number of species, and proved to be economically viable in cattle. in the last two decades, various newly developed reproductive technologies applicable in buffaloes have mushroomed. recently, following the birth of the first buffalo calves using ai with sexed semen, commercial interest to exploit sexing of semen in this species too is aroused. in order t ... | 2011 | 21497388 |
| microisolation and microcloning of bovine x-chromosomes for identification of sorted buffalo (bubalus bubalis) spermatozoa. | flow-cytometry sorting technology has been successfully used to separate the x- and y-chromosome bearing spermatozoa for production of sex-preselected buffalo. however, an independent technique should be employed to validate the sorting accuracy. in the present study, x-chromosomes of bovine were micro-dissected from the metaphase spreads by using glass needles. then x-chromosomes were then amplified by pcr and labelled with cy3-dutp for use as a probe in hybridization of the unsorted and sorted ... | 2011 | 21640528 |
| attenuation of luteolytic response following fish meal supplementation in dairy buffaloes (bubalus bubalis). | luteolysis of corpus luteum, due to un-inhibited pgf(2α) secretion, has been reported to be a cause of early embryonic mortality in dairy animals. the objective of this study was to determine the effects of fish meal (fm) supplementation on the uterine secretion of pgf(2α) and hence establish its supplementation as an antiluteolytic strategy in dairy buffaloes. five cycling murrah buffaloes were supplemented with 250g fm daily for 55 days in addition to their routine feed and seven buffaloes wer ... | 2011 | 21641133 |
| follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (bubalus bubalis). | the objective of this study was to examine the follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in buffaloes. ovaries were collected from 56 acyclic and 95 cyclic buffaloes at slaughter, surface follicle number was counted and follicles were classified into small (5.0-6.9 mm), medium (7.0-9.9 mm), and large (≥10.0 mm) size categories based on their diameter. follicular fluid was aspirated and assayed for nitric oxide, ascor ... | 2011 | 21643661 |
| characterization of enterotoxigenic e. coli (etec), shiga-toxin producing e. coli (stec) and necrotoxigenic e. coli (ntec) isolated from diarrhoeic mediterranean water buffalo calves (bubalus bubalis). | two hundred and twenty escherichia coli isolates from 314 mediterranean water buffalo calves less than 4weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors lt, st, stx1, stx2, haemolysins, intimin, cnf1, cnf2, cdt-i, cdt-ii, cdt-iii, cdt-iv, and f17-related fimbriae (f17a, f17b, f17c, f17d). the prevalence of etec, stec and ntec were 1.8%, 6.8% and 20.9%, respectively. the etec isolates were all lt-positive and st-negative. th ... | 2011 | 21658736 |
| molecular prevalence of different genotypes of theileria orientalis detected from cattle and water buffaloes in thailand. | abstract here, we report on an epidemiological study regarding the molecular prevalence of different genotypes of theileria orientalis present among domestic cattle and water buffalo populations bred in thailand. a phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein (mpsp) revealed the presence of 5 genotypes (types 1, 3, 5, 7, and n-3) in cattle and 7 genotypes (types 1, 3, 4, 5, 7, n-2, and n-3) in water buffaloes. types 4, 7, and n-3 of t. orientalis w ... | 2011 | 21671722 |
| presence of nitric oxide synthase immunoreactivity and mrna in buffalo (bubalus bubalis) oocytes and embryos. | this study examined the presence of immunoreactivity and mrna for different nitric oxide synthase (nos) isoforms in immature and in vitro matured oocytes and in embryos at two-, four- and eight-cell, and morula and blastocyst stages in buffalo. oocytes obtained from slaughterhouse buffalo ovaries were subjected to in vitro maturation in tcm-199 + 10% fbs + 5 µg/ml pfsh + 1 µg/ml estradiol-17ß + 0.81 mm sodium pyruvate + 10% buffalo follicular fluid + 50 µg/ml gentamycin sulphate for 24 h in a co ... | 2011 | 21793945 |
| in vitro immune competence of buffaloes (bubalus bubalis) of different production potential: effect of heat stress and cortisol. | twelve healthy lactating murrah buffaloes of similar parity (3rd) between 90 and 120 days of lactation, selected from the herd of national dairy research institute (karnal, india) and maintained at managemental practices as followed at the institute they were included in this experiment. the animals were divided into two groups based on their production level in previous lactation. the average milk production level of group 1 and ii was 9.3 and 6?lit/day, respectively. blood was collected from t ... | 2011 | 21776354 |
| somatotropin-mediated gene expression profiling of differentially displayed ests during lactation in indian buffalo (bubalus bubalis). | the study of bovine mammary gland functional genomics requires appropriate cdna library collections to access gene expression patterns from different developmental and physiological stages. the present study was undertaken with the objective to identify candidate genes involved in the process of increased milk synthesis following 0, 48 and 96 h of recombinant bovine somatotropin (rbst) treatment to surti buffalo (bubalus bubalis) through differential display reverse transcriptase pcr (ddrt-pcr). ... | 2011 | 21774858 |
| sequence characterization of s100a8 gene reveals structural differences of protein and transcriptional factor binding sites in water buffalo and yak. | the present study was undertaken to characterize the structure of s100a8 gene and its promoter in water buffalo and yak. sequence data of 2.067 kb, 2.071 kb, and 2.052 kb with respect to complete s100a8 gene including 5' flanking region was generated in river buffalo, swamp buffalo, and yak, respectively. blast analysis of coding dna sequences (cds) of s100a8 gene revealed 95% homology of buffalo sequence with cattle, 85% with pig and horse, 83% with dog, 72-73% with murines, and around 79% with ... | 2011 | 21774620 |
| effects of recipient oocyte age and interval from fusion to activation on development of buffalo (bubalus bubalis) nuclear transfer embryos derived from fetal fibroblasts. | the objective was to investigate the effect of recipient oocyte age and the interval from activation to fusion on developmental competence of buffalo nuclear transfer (nt) embryos. buffalo oocytes matured in vitro for 22 h were enucleated by micromanipulation under the spindle view system, and a fetal fibroblast (pretreated with 0.1 µg/ml aphidicolin for 24 h, followed by culture for 48 h in 0.5% fetal bovine serum) was introduced into the enucleated oocyte, followed by electrofusion. both oocyt ... | 2011 | 21752448 |
| maturation competence of swamp buffalo oocytes obtained by ovum pick-up and from slaughterhouse ovaries. | this study was designed with the final goal of improving in vitro embryo production in the thai swamp buffalo (bubalus bubalis carabensis). oocytes were collected by ovum pick-up (opu) from six non-lactating multiparous swamp buffalo twice per week for 10 consecutive sessions followed by once-weekly collection for 10 consecutive sessions without hormone stimulation. in addition, oocytes were collected from slaughterhouse ovaries that were classified as follows: ovaries from non-pregnant cows wit ... | 2011 | 21749483 |
| genetic diversity of mitochondrial cytochrome b gene in chinese native buffalo. | the origins of the domestic water buffalo remain contentious. to better understand the origins of chinese water buffalo, we sequenced the complete mitochondrial cytochrome b (mt-cyb) gene from 270 individuals representing 13 chinese domestic swamp buffalo populations. we found genetic evidence of introgression of river buffalo into chinese swamp buffalo herds. swamp buffalo haplotypes can be divided into two highly divergent lineages (a and b), suggesting that chinese native swamp buffalo have t ... | 2011 | 21749426 |
| polymorphism and gene organization of water buffalo mhc-dqb genes show homology to the bola dqb region. | in cattle (bos taurus), there is evidence of more than 50 alleles of bola-dqb (bovine lymphocyte antigen dqb) that are distributed across at least five dqb loci, making this region one of the most complex in the bola gene family. in this study, dqb alleles were analysed for the water buffalo (bubalus bubalis), another economically important bovine species. twelve alleles for bubu-dqb (bubalis bubalis dqb) were determined by nucleotide sequence analysis. a phylogenetic analysis revealed numerous ... | 2011 | 21749420 |
| genetic differentiation of water buffalo (bubalus bubalis) populations in china, nepal and south-east asia: inferences on the region of domestication of the swamp buffalo. | data from three published studies of genetic variation at 18 microsatellite loci in water buffalo populations in china (18 swamp type, two river type), nepal (one wild, one domestic river, one hybrid) and south-east asia (eight swamp, three river) were combined so as to gain a broader understanding of genetic relationships among the populations and their demographic history. mean numbers of alleles and expected heterozygosities were significantly different among populations. estimates of ? (a me ... | 2011 | 21749419 |
| clinical, hematological, and biochemical findings of uterine torsion in buffaloes (bubalus bubalis). | the aim of this study was to investigate uterine torsion in buffaloes, examine factors influencing the outcome of the disease, and to characterize the related alterations in blood constituents. a total of 126 buffaloes with uterine torsion were examined for stage of gestation, duration, degree, site and direction of torsion, as well as the location of the pregnant horn. methods of correction were documented along with dam and calf survival. blood samples were obtained from 20 buffaloes with uter ... | 2011 | 21676564 |
| characterization of prlr and ppargc1a genes in buffalo (bubalus bubalis). | more than 40 million households in india depend at least partially on livestock production. buffaloes are one of the major milk producers in india. the prolactin receptor (prlr) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (ppargc1a) gene are reportedly associated with milk protein and milk fat yields in bos taurus. in this study, we sequenced the prlr and ppargc1a genes in the water buffalo bubalus bubalis. the prlr and ppargc1a genes coded for 581 and 819 amino ac ... | 2011 | 22215963 |
| experimental inoculation of neospora caninum in pregnant water buffalo. | the aim of this study was to characterize the pathogenesis of neospora caninum in experimentally inoculated pregnant water buffalo (bubalus bubalis). twelve mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to n. caninum by indirect fluorescent antibody test (ifat) were involved. ten females were intravenously inoculated with 10(8) tachyzoites of nc-1 strain at 70 (n=3) or 90 (n=7) days of pregnancy (dp). two control animals were inoculated with placebo ... | 2011 | 22244534 |
| natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation. | this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ... | 2011 | 22654314 |
| effects of plane of nutrition on slaughtering traits and meat characteristics in murrah graded male buffalo (bubalus bubalis) calves in nepal. | an experiment was conducted using 17 male buffalo calves to assess the effects of plane of nutrition on slaughtering traits and meat characteristics. to attain 250 kg body weight (bw), the calves were allocated into three groups: high (h), low-high (l-h) and low (l) corresponding to concentrate levels receiving the concentrate at 1.50% of bw, 0.75% of bw until 190 kg bw and 1.50% thereafter, and 0.75% of bw, respectively. the animals had ad libitum access to urea-treated rice straw. no significa ... | 2011 | 22574796 |
| mitochondrial dna diversity patterns in pakistani buffalo. | the indian subcontinent is considered to be the likely centre of river buffalo domestication, based on population dynamics, archaeological evidence and genetic diversity. recent studies on mitochondrial dna diversity have drawn useful conclusions about the domestication history of bubalus bubalis. the conclusions of these studies are, however, incomplete, unless samples can also be analysed from pakistan, which contains the second largest buffalo population of the world. here, we report the resu ... | 2011 | 22486503 |
| effect of incorporation of additives in tris-based egg yolk extender on buffalo (bubalus bubalis) sperm tyrosine phosphorylation during cryopreservation. | phosphorylation of tyrosine residues on sperm protein is a known indicator of capacitation and a major intracellular signalling event. there is evidence that sperm cryopreservation promotes tyrosine phosphorylation and is associated with reduced fertility of spermatozoa. under this study, cryoprotective role of different additives namely taurine, trehalose, catalase and 4-bromophenacyl bromide on buffalo sperm quality was evaluated. buffalo semen was cryopreserved in tris-based egg yolk extender ... | 2011 | 22364363 |
| phenotype characterization of staphylococcus species strains isolated from buffalo (bubalus bubalis) milk. | the objective of the current study was to isolate and identify phenotypes of staphylococcus spp. strains derived from buffalo (bubalus bubalis) milk. a total of 548 milk samples from 137 buffalo were cultured in columbia agar enriched with 5% defibrinated sheep blood. determination of the capacity of staphylococcus aureus to produce enterotoxins a-d and toxic shock syndrome toxin-1 (tsst-1) was achieved by reverse passive latex agglutination (rpla). antimicrobial sensitivity of s. aureus strains ... | 2011 | 22362803 |
| soya-lecithin in extender improves the freezability and fertility of buffalo (bubalus bubalis) bull spermatozoa. | egg yolk is routinely used as a cryoprotectant in semen extenders. however, it may contain cryoprotective antagonists, and there are hygienic risks associated with its use. proteins of plant origin, like soya-lecithin, lack these hazards. the aim of this study was to use soya-lecithin as a cryoprotectant in extender and to investigate its effects on in vitro quality and in vivo fertility of buffalo semen. semen from three buffalo bulls was frozen in tris-citric extender containing 5.0%, 10% or 1 ... | 2011 | 22211278 |
| characterization and in silico analysis of pregnancy-associated glycoprotein-1 gene of buffalo (bubalus bubalis). | pregnancy-associated glycoproteins (pags) are trophoblastic proteins belonging to the aspartic proteinase family secreted by different placental cells of many mammalian species. they play a pivotal role in placentogenesis, foetomaternal unit remodeling, and implantation. the identification of the genes encoding those proteins will be helpful to unravel the intricate embryogenomic functions during pregnancy establishment. considering importance of these proteins, the present study was undertaken ... | 2011 | 22567354 |
| Metagenomic analysis of Surti buffalo (Bubalus bubalis) rumen: a preliminary study. | The complex microbiome of the rumen functions as an effective system for the conversion of plant cell wall biomass to microbial proteins, short chain fatty acids and gases. In this study, metagenomic approaches were used to study the microbial populations and metabolic potential of the microbial community. DNA was extracted from Surti Buffalo rumen samples (four treatments diet) and sequenced separately using a 454 GS FLX Titanium system. We used comparative metagenomics to examine metabolic pot ... | 2011 | 21947953 |
| Multilocus phylogeographical analysis of Trypanosoma (Megatrypanum) genotypes from sympatric cattle and water buffalo populations supports evolutionary host constraint and close phylogenetic relationships with genotypes found in other ruminants. | Species of the subgenus Trypanosoma (Megatrypanum) have been reported in cattle and other domestic and wild ruminants worldwide. A previous study in Brazil found at least four genotypes infecting cattle (Bos taurus), but only one in water buffalo (Bubalus bubalis). However, the small number of isolates examined from buffalo, all inhabiting nearby areas, has precluded evaluation of their diversity, host associations and geographical structure. To address these questions, we evaluated the genetic ... | 2011 | 22051399 |
| effect of selenium, zinc, and copper supplementation on blood metabolic profile in male buffalo (bubalus bubalis) calves. | twenty male buffalo calves (15 months, 200.2 ± 9.75) were divided into four groups of five animals in each and fed diets without (t1) or supplemented with 0.3 ppm selenium (se) + 40 ppm zinc (zn) (t2), 0.3 ppm se + 40 ppm zn + 10 ppm copper (cu) (t3), and 40 ppm zn + 10 ppm cu (t4) for 120 days, during which blood samples were collected on days 0, 40, 80, and 120. concentrations of glucose, total protein, albumin, globulin, urea, uric acid, and creatinine were similar in all the four groups. the ... | 2011 | 21947859 |
| cloning and sequencing of indian water buffalo (bubalus bubalis) interleukin-3 cdna. | full-length cdna (435 bp) of the interleukin-3(il-3) gene of the indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. this sequence had 96% nucleotide identity and 92% amino acid identity with bovine il-3. there are 10 amino acid substitutions in buffalo compared with that of bovine. the amino acid sequence of buffalo il-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. structural homology modell ... | 2011 | 22152058 |
| Molecular mining of alleles in water buffalo Bubalus bubalis and characterization of the TSPY1 and COL6A1 genes. | Minisatellites are an integral part of eukaryotic genomes and show variation in the complexity of their organization. Besides their presence in non-coding regions, a small fraction of them are part of the transcriptome, possibly participating in gene regulation, expression and silencing. We studied the minisatellite (TGG)(n) tagged transcriptome in the water buffalo Bubalus bubalis across various tissues and the spermatozoa, and characterized the genes TSPY1 and COL6A1 discovered in the process. | 2011 | 21949806 |
| baccharis megapotamica var. weirii poisoning in water buffalo (bubalus bubalis). | an outbreak of an acute disease in buffalo (bubalus bubalis) caused by the ingestion of baccharis megapotamica var. weirii occurred in the southern region of brazil. ten out of 50 buffalo died 24-48 hr after being introduced into a pasture containing abundant amounts of the plant. factors influencing the ingestion of the plant and consequent toxicosis included hunger, stress caused by shipment, and unfamiliarity with the plant. clinical signs included serous ocular discharge, incoordination, mil ... | 2011 | 21908301 |
| Isolation, culturing and characterization of feeder-independent amniotic fluid stem cells in buffalo (Bubalus bubalis). | Heterogeneous amniotic fluid contains various cell types. The aim of this study was to characterize and differentiate some of the key stemness attributes of the amniotic fluid-derived cells in buffalo (Bubalus bubalis). The amniotic fluid (AF) cells were cultured without feeder cells, in DMEM containing 15% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin/ampicillin, 1% vitamin solution, and 1% l-glutamine in 5% CO(2) in humidified air at 38.5±0.5°C. After 6days of culture different ... | 2011 | 21982462 |
| molecular modeling and docking characterization of dectin-1 (pamp) receptor of bubalus bubalis. | dectin-1, is a type ii transmembrane receptor protein which contains a single extracellular ctld (c-type lectin domain), stalk, transmembrane domain and an itam (immunoreceptor tyrosine-based activation motifs) in its cytoplasmic tail. dectin-1 has the ability to recognize fungal β-glucans, which are carbohydrate pamps found predominantly in fungal cell walls. the recognition of fungal β-glucans by dectin-1 helps in a variety of cellular responses, like host protection, such as fungal uptake and ... | 2011 | 22015804 |
| comparative proteomics to evaluate multi drug resistance in escherichia coli. | drug resistance in food-borne bacterial pathogens is an almost inevitable consequence of the use of antimicrobial drugs, used either therapeutically or to avoid infections in food-producing animals. in the past decades, the spread and inappropriate use of antibiotics have caused a considerable increase of antibiotics to which bacteria have developed resistance and, moreover, bacteria are becoming resistant to more than one antibiotic simultaneously. understanding mechanisms at the molecular leve ... | 2011 | 22120138 |
| a rapid method for authentication of buffalo (bubalus bubalis) meat by alkaline lysis method of dna extraction and species specific polymerase chain reaction. | buffalo (bubalus bubalis) meat is a major item of export from india but export of beef i.e. meat from cattle (bos indicus) is prohibited. also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of india. food analysts require precise identification techniques to implement such regulations. in the present study, a method of dna extraction by alkaline lysis from meat samples and speciation of buf ... | 2011 | 24425899 |
| immunohistochemical studies of the epididymal duct in egyptian water buffalo (bubalus bubalis). | using immunohistochemistry (ihc), this study aimed to evaluate the regional distribution pattern of some biologically active proteins in the epididymis of egyptian water buffalo and to determine the structural-functional relationships of the different epididymal structures. wax-embedded sections from different regions of the epididymal duct from adult, clinically healthy, buffalo bulls were used. primary antibodies against angiotensin converting enzyme (ace), s-100, galactosyltransferase (galtas ... | 2011 | 19836061 |
| detection of follicular apoptosis in water buffalo (bubalus bubalis) ovary by histology and nick end labelling technique. | the objective of this experiment was to assess the features and extent of follicular apoptosis in the water buffalo (bubalus bubalis) ovary using classical histology and nick end labelling technique. ovaries (n=40) procured from the slaughterhouse were used for the study. the sections (5 μm) were used for detection of terminal deoxynucleotidyl transferase-mediated dutp-biotin nick end labelling (tunel) and classical histology (h&e). those follicles showing ≥ 5% tunel positivity (tunel assay) and ... | 2011 | 20070580 |
| in vitro evaluation of liquid-stored buffalo semen at 5°c diluted in soya lecithin based extender (bioxcell®), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. | this study was designed to compare the quality of liquid-stored buffalo bull spermatozoa in soya lecithin based extender bioxcell(®) (biox), milk (milk), tris-citric egg yolk (tey) and egg yolk-citrate (eyc) extender at 5°c. semen was collected from five nili-ravi buffalo (bubalus bubalis) bulls of 6-7 years of age with artificial vagina over a period of 3 weeks (two consecutive ejaculates once in a week). semen ejaculates having more than 60% motility were pooled, split into four aliquots, dilu ... | 2011 | 20070582 |
| buffalo (bubalus bubalis) embryonic stem cell-like cells and preimplantation embryos exhibit comparable expression of pluripotency-related antigens. | in this study, inner cell mass (icm) cells were isolated from in vitro produced buffalo blastocysts and were cultured on mitomycin-c treated buffalo foetal fibroblast feeder layer for producing embryonic stem (es) cells. among different sources (hatched vs expanded blastocysts) or methods (enzymatic vs mechanical), mechanical isolation of icm from hatched blastocysts resulted in the highest primary colony formation rate and the maximum passage number up to which es cells survived. putative es ce ... | 2011 | 20042025 |
| effects of chemical activation treatment on development of swamp buffalo (bubalus bubalis) oocytes matured in vitro and fertilized by intracytoplasmic sperm injection. | the objective of this study was to optimize the activation protocol for buffalo oocytes after intracytoplasmic sperm injection (icsi). the release of the second polar body (pb) at 3, 6 and 9 h after icsi of in-vitro matured oocytes activated either with 5 μm ionomycin (io) or with 7% ethanol (etoh) was preliminary examined. the highest rate of second pb extrusion occurred at 3 h of activation, and the second pb extrusion in etoh group was significantly higher than that in io group. oocytes that ... | 2011 | 20546174 |
| follicular dynamics and oestrous detection in thai postpartum swamp buffaloes (bubalus bubalis). | this study characterized follicular activity and oestrous behaviour from 5 to 9 days post-calving up to the 4th ovulation postpartum (pp) in 16 multiparous (range 2-7 parities) thai swamp buffalo cows (bubalus bubalis), aged 4-12 years and weighing from 432 to 676 kg. ovarian follicular activity was examined by transrectal ultrasonography (tus) every morning. oestrous detection was performed twice daily by direct personal observation of behaviour and for presence of clear cervical mucus discharg ... | 2011 | 20546176 |
| quantification, morphology and ultrastructure of preantral follicles of buffalo (bubalus bubalis) foetuses. | the objective of this study was to determine the number, morphology and ultrastructure of preantral ovarian follicles of buffalo (bubalus bubalis) foetuses at different ages. quantification revealed number of primordial, primary and secondary follicles of 48,857 ± 17,506, 26,000 ± 20,452, 18,428 ± 10,875 and 18,375 ± 19,690, 225 ± 349, 326 ± 288 at 12-34 cm and 35-60 cm crown rump length (crl), respectively. follicular diameter values were 28.9 (± 3.4), 34.7 (± 5.9) and 59.4 (± 12.6) μm; oocyte ... | 2011 | 20403125 |
| localization and expression of follicle-stimulating hormone receptor gene in buffalo (bubalus bubalis) pre-antral follicles. | follicle-stimulating hormone (fsh) stimulates antral follicles to grow, but its role in earlier stages (pre-antral) of follicle development, if any, is obscure. aim of this study was to study the expression of follicle-stimulating hormone receptor (fshr) gene in different sizes of pre-antral follicles (pfs) (<150, 200, 250, 300, 350, 400 μm) and to find out an optimum dose of fsh for better growth, development and steroidogenesis of pfs in vitro. buffalo ovaries were collected from a local abatt ... | 2011 | 20403128 |
| effect of pineal proteins at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in rats. | pineal glands secrets melatonin and various proteins and peptides which has many physiological functions. in keeping with this view, present experiment was conducted to know the effect of buffalo (bubalus bubalis) pineal proteins (pp) at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in female rats. for this, we took 30 adult female wistar rats (133-145 g body weights, bw) and divided into five groups (control, group i; 150 ppm fluoride (f) ... | 2011 | 20509005 |
| a rare case of centric fission and fusion in a river buffalo (bubalus bubalis, 2n = 50) cow with reduced fertility. | a 5-year-old river buffalo cow underwent cytogenetic investigation since it had only one male offspring, apparently with normal body constitution, which died one month after birth. the female carrier had normal body conformation and internal sex adducts, as revealed by rectal palpation performed by a specialist veterinary practitioner. the cow was found to carry a complex and rare chromosome abnormality. indeed, a centric fission of one river buffalo (bbu) chromosome 1 with a subsequent (or simu ... | 2011 | 20693780 |
| comparative potential of cultured skin fibroblast, cumulus, and granulosa cell to produce somatic cell nuclear transfer (scnt) preimplantation embryos in buffaloes (bubalus bubalis) in relation to gene expressions. | to improve the efficiency of somatic cell nuclear transfer (scnt)-derived embryos in buffaloes (bubalus bubalis), skin fibroblast, cumulus, and granulosa cells were cultured up to the 15th passage and cloned embryos were produced from each cell type. at the 15th passage the cumulative population doublings (cpds) in cumulus cells was higher (60.78) than skin fibroblast (57.12) and granulosa (56.05) cell lines. gene expression of chromatin remodelling proteins, that is, hdac1, dnmt1, dnmt3a, and d ... | 2010 | 20698775 |
| spermatogonia-specific proteins expressed in prepubertal buffalo (bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia. | buffalo is an economically important livestock species in asia. little is known about male germ line technology owing to lack of sufficient understanding regarding expression of germ- and somatic-cell specific-proteins in the testis. in this study, we identified uchl-1 (pgp 9.5) and lectin- dolichos biflorus agglutinin (dba) as specific markers for spermatogonia in buffalo testis. expression of germ-cell and pluripotency-specific proteins such as ddx4 (vasa) and pou5f1 (oct3/4) were also present ... | 2010 | 20708247 |
| radionuclide concentration ratios in australian terrestrial wildlife and livestock: data compilation and analysis. | radionuclide concentrations in australian terrestrial fauna, including indigenous kangaroos and lizards, as well as introduced sheep and water buffalo, are of interest when considering doses to human receptors and doses to the biota itself. here, concentration ratio (cr) values for a variety of endemic and introduced australian animals with a focus on wildlife and livestock inhabiting open rangeland are derived and reported. the cr values are based on u- and th-series concentration data obtained ... | 2010 | 20711841 |
| bacterial diversity in the rumen of indian surti buffalo (bubalus bubalis), assessed by 16s rdna analysis. | bacterial communities in buffalo rumen were characterized using a culture-independent approach for a pooled sample of rumen fluid from 3 adult surti buffaloes. buffalo rumen is likely to include species of various bacterial phyla, so 16s rdna sequences were amplified and cloned from the sample. a total of 191 clones were sequenced and similarities to known 16s rdna sequences were examined. about 62.82% sequences (120 clones) had >90% similarity to the 16s rdna database sequences. furthermore, ab ... | 2010 | 20720314 |
| prevalence, biology, and distribution pattern of sarcocystis infection in water buffalo (bubalus bubalis) in iran. | this study was conducted to determine the prevalence, distribution pattern, and the sarcocystis species involved in slaughtered water buffaloes (bubalus bubalis) in the khuzestan, iran by macroscopic and histological examination. the esophagus, heart, diaphragm, tongue, masseter, and thigh muscles were investigated. esophagus and thigh muscles of only 3 of the 100 examined water buffaloes (3%) were infected with macroscopic sarcocystis, whereas at microscopic level sarcocystis were found in 83 o ... | 2010 | 20524065 |
| progesterone supplementation during multiple ovulation treatment in buffalo species (bubalus bubalis). | the aim of this study was to evaluate the effect of exogenous progesterone supplementation on superovulatory response in buffaloes that has undergone a multiple ovulation program. fourteen mediterranean buffaloes were divided into two groups and received a 4-day decreasing dosage of an equal mixture of 500 iu of fsh and lh starting on day 8 of the cycle. in group a (n = 7) a progesterone-releasing intravaginal device was removed on day 8, whereas in group b (n = 7) it was left till day 10, when ... | 2010 | 20411328 |
| risk factors associated with the presence of positive reactions in the sccit test in water buffalo around two cities in punjab, pakistan. | the present study was undertaken to find out the association of certain risk factors with the positive sccit (single comparative cervical intradermal tuberculin) test in water buffaloes at faisalabad and okara, pakistan. seventy-six herds (697 buffaloes) at faisalabad and 56 (395 buffaloes) at okara were included in the study. the buffaloes were screened through sccit test. positive reaction to sccit test was recorded in 14% of herds and in 3% of buffaloes. the herds positive for this test were ... | 2010 | 20412784 |
| hot topic: gene duplication at the α-lactalbumin locus: finding the evidence in water buffalo (bubalus bubalus l.). | studies on milk proteins revealed that a qualitative and quantitative polymorphism may often be found regarding alpha-lactalbumin (alpha-la). in mammals, a similar phenomenon was widely documented in the alpha-globin system as the result of a gene duplication. the presence of several differently expressed alpha-lactalbumin gene (lalba) products suggests that the mechanism underlying this phenomenon may involve nonallelic genes. to check this hypothesis, an experiment was set up to investigate th ... | 2010 | 20412931 |
| cellular coupling of potassium channels with beta2 adrenoceptors in mediating myometrial relaxation in buffaloes (bubalus bubalis). | present study unravels the functional presence of potassium channels and their involvement in mediating beta(2) adrenoceptors-induced myometrial relaxation in buffalo myometrium. isolated myometrial preparations exhibited rhythmic spontaneity with regular pattern of amplitude and frequency. levcromakalim produced concentration-dependent inhibitory effect on myometrial spontaneity and relaxant effect and the dose-response curve (drc) was shifted towards right in the presence of glybenclamaide. in ... | 2010 | 20444021 |
| genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes. | river buffalo (bubalus bubalis, 2n = 50, bbu) is a species of economic relevance in a number of countries. this species shows a very peculiar biology and a great capacity for environmental adaptation. there has been an increasing economic interest as well as a growing demand for a more detailed knowledge of molecular features in this species. from this perspective we report a genomic, transcriptional and cytogenetic analysis of 5 master genes involved in skeletal muscle development. of these 5 g ... | 2010 | 20453476 |
| genome-wide search of the genes tagged with the consensus of 33.6 repeat loci in buffalo bubalus bubalis employing minisatellite-associated sequence amplification. | minisatellites have been implicated with chromatin organization and gene regulation, but mrna transcripts tagged with these elements have not been systematically characterized. the aim of the present study was to gain an insight into the transcribing genes associated with consensus of 33.6 repeat loci across the tissues in water buffalo, bubalus bubalis. using cdna from spermatozoa and eight different somatic tissues and an oligo primer based on two units of consensus of 33.6 repeat loci (5' cct ... | 2010 | 20480223 |
| characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (bubalus bubalis) calves. | recently, a coronavirus strain (179/07-11) was isolated from water buffalo (bubalus bubalis) and the virus which displayed a strict genetic and biological relatedness with bovine coronavirus (bcov) was referred to as bubaline coronavirus (bucov). here, we report the characterisation of four bucovs strains identified in the faeces or intestinal contents of water buffalo calves with acute gastroenteritis. single bucov infections were detected in all but one cases from which two clostridia species ... | 2010 | 20483547 |