Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. | a panel of twenty monoclonal antibodies to the hemagglutinin-neuraminidase (hn) glycoprotein of human parainfluenza 3 (pi3) virus has been obtained and tentatively classified into four different groups based on reactivity in hemagglutination inhibition (hi), neuraminidase inhibition (ni), and plaque neutralization (nt) tests. the antibodies were tested for cross-reactivity with bovine pi3 virus, sendai virus, and simian virus 5 (sv5). only two of these antibodies showed similar reactivities with ... | 1986 | 2417412 |
| [response of guinea pigs to vaccination with parainfluenza virus 3]. | an inactivated vaccine was prepared with parainfluenza-3 virus strain lq-514 and strains of pasteurella hemolytica and p. multocida, suspended in oil adjuvant. the virus had been isolated from 30-60 day old calves during an epidemic of pneumonia. the vaccine was tested in guinea pigs aged 1 to 2 months. the antibody response and the virus titres in organs after the challenge were the parameters studied. hemagglutination inhibition antibodies were first detected 14 days after vaccination and reac ... | 1985 | 2829271 |
| antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. | sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (hn) protein of a 1957 isolate of parainfluenza type 3 virus (piv3) were produced and used to examine antigenic variation in clinical strains. analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in the presence of monoclonal antibodies indicated that there were a minimum of six distinct epitopes detectable on the hn molecule. competitive-binding assays indicated that these ep ... | 1985 | 2414910 |
| glycoproteins of human parainfluenza virus type 3: characterization and evaluation as a subunit vaccine. | the envelope glycoproteins of human parainfluenza type 3 virus were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reactivity with specific monoclonal antibodies. the molecular weight of the hemagglutinin-neuraminidase (hn) glycoprotein was found to be 72,000, and the fusion (f) glycoprotein appeared to consist of 74,000 (f0) or 56,000 (f1) species. envelope glycoproteins were solubilized with octyl-glucoside and, after removal of the detergent by dialysis, were u ... | 1985 | 2999259 |
| in vitro protective effect of bacteria-derived bovine alpha interferon i1 against selected bovine viruses. | we used bacteria-derived bovine alpha-interferon i1 (bo ifn-alpha i1) to study its antiviral effect in a bovine turbinate cell line on bovine diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and pseudorabies virus. we based our study upon replicate tests for each strain by using a block titration system with various concentrations of bo ifn-alpha i1 against various concentrations of virus. the data were compiled in two-axis tables (replicate x concentration) and we ... | 1985 | 2999188 |
| serum antibody to respiratory syncytial virus in goats in the uk. | 1985 | 2988182 | |
| the importance of imp dehydrogenase inhibition in the broad spectrum antiviral activity of ribavirin and selenazofurin. | the nucleosides ribavirin, selenazofurin, tiazofurin and bredinin all exhibit the lowering of guanylate pools in vitro and in vivo by the inhibition of imp dehydrogenase. however, each of these nucleosides has a separate profile of antiviral and antitumor activity. the imp dehydrogenase inhibition in the case of ribavirin and bredinin appears to be due to the nucleoside 5'-monophosphate and in the case of selenazofurin and tiazofurin to the nad analogs formed intracellularly. with regard to the ... | 1985 | 2872781 |
| inability to detect a k cell in bovine peripheral blood leukocytes. | antibody-dependent cellular cytotoxicity to viral-infected cells, chicken red blood cells, and tumor cells was tested using different effector cell populations: polymorphonuclear cells, mononuclear cells, and mononuclear cells separated into adherent and nonadherent populations by sephadex g-10. polymorphonuclear cells were the most efficient mediators of antibody-dependent cellular cytotoxicity against most targets, although a combination of g-10 adherent and polymorphonuclear cells was more ef ... | 1985 | 2988185 |
| respiratory virus surveillance--united states, january 1985. | 1985 | 2981395 | |
| in vivo and in vitro studies on the use of the guinea pig as a model for virus-provoked airway hyperreactivity. | the parainfluenza 3 (p-3)-infected guinea pig was examined as a model for virus-provoked airway hyperreactivity by measuring changes in airway overflow pressure in response to intravenously (iv) administered histamine. in vitro responses of lung parenchymal strips to several contractile substances were also measured. all studies were conducted 4 days after nasal insufflation with p-3 or p-3 growth medium (control). increases in airway overflow pressure caused by histamine were enhanced by p-3 in ... | 1985 | 2992331 |
| antiviral effect of flavonoids on human viruses. | the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ... | 1985 | 2981979 |
| kawasaki disease associated with klebsiella pneumoniae bacteremia and parainfluenza type 3 virus infection. | 1985 | 2982131 | |
| synthesis and biological activity of 5-thiobredinin and certain related 5-substituted imidazole-4-carboxamide ribonucleosides. | a number of 5-substituted imidazole-4-carboxamide ribonucleosides were prepared and tested for their biological activity. treatment of 5-chloro-1-beta-d-ribofuranosylimidazole-4-carboxamide (2) with methanethiol provided 5-(methylthio)-1-beta-d-ribofuranosylimidazole-4-carboxamide (3a). similar treatment of 2 with ethanethiol or benzenemethanethiol gave the corresponding 5-ethylthio and 5-benzylthio derivatives 3b and 3c. oxidation of 3a and 3b with m-chloroperoxybenzoic acid furnished the corre ... | 1985 | 2993613 |
| proteins associated with human parainfluenza virus type 3. | the polypeptides associated with human parainfluenza virus type 3 were identified. five proteins were present in detergent- and salt-resistant viral cores. of these, three proteins designated np0, np1, and np2 of 68,000, 58,000, and 52,000 daltons, respectively, were stably associated with 50s rna in cscl gradient-purified nucleocapsids. the amounts of np1 and np2 were variable, and these proteins were shown to be structurally related to the major nucleocapsid protein (np0) by partial staphyloco ... | 1985 | 2993658 |
| rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions. | nasopharyngeal secretions from adults and children were obtained in stockholm, sweden, for routine diagnosis of influenza a virus, influenza b virus, respiratory syncytial (rs) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. the cells in nasopharyngeal secretions were pelleted by centrifugation for preparation of cell deposits for diagnosis by the immunofluorescence technique (if) in london, england, and in stockholm, whe ... | 1985 | 2997270 |
| experimental parainfluenza virus infection in mice: growth and spread of a highly pathogenic variant of parainfluenza 3 virus in the mouse brain. | we had previously showed that following intracerebral inoculation of newborn mice, the 910 n and m strains of bovine parainfluenza 3 virus induce a non-lethal hydrocephalus and a lethal disease with marked thymic and splenic atrophy, respectively. moreover, only the m virus was lethal for 2-week-old mice. in the present study, we demonstrate that the m virus multiplies and spreads in the mouse brain invading the thalamus, hypothalamus and brain stem beyond the ependyma whereas the 910 n virus ca ... | 1985 | 2982350 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| properties of temperature-sensitive mutants of parainfluenza virus type 3 selected during the course of persistent infection. | we investigated properties of the ts mutants that were selected during the course of persistent infection of vero cells by parainfluenza virus type 3. the mutants demonstrated leakiness when infecting cells at high moi and interfered with the growth of wild type virus, apparently by inhibiting a step prior to rna synthesis. | 1985 | 2986583 |
| studies on human parainfluenza virus 3: characterization of the structural proteins and in vitro synthesized proteins coded by mrnas isolated from infected cells. | the structural proteins of human parainfluenza virus 3, a member of the paramyxovirus family, were characterized by sds-polyacrylamide gel electrophoresis of radiolabeled virus. the purified virion contains at least eight structural proteins, with estimated molecular weights of 251k, 90k, 71k, 68k, 65k, 51k, 35k, and 21k, respectively. three of the polypeptides (71k, 65k, and 51k) were identified as glycoproteins based on their incorporation of [3h]glucosamine. disruption of the virus by triton ... | 1985 | 2998013 |
| cloning and gene assignment of mrnas of human parainfluenza virus 3. | cytoplasmic poly(a)-containing rna from parainfluenza virus 3-infected cells was used as template to construct a cdna library that was cloned into the ecorv site of the plasmid pmg5. the resulting clones were screened with [32p]-labeled cdna probes made from infected and mock-infected cell mrnas. the virus specificity of the clones was confirmed by northern blot hybridization. the viral clones were grouped into five different families by hybridization with individual size-selected reverse transc ... | 1985 | 2998060 |
| a serologic epidemiological study of parainfluenza 1, 2, and 3 viruses in beef cattle and calves in illinois. | beef cattle and calves in four illinois populations were studied. serum was tested by the hemagglutination-inhibition (hi) test for antibodies to parainfluenza viruses (piv) 1, 2, and 3. in all but group 3 a low level of antibody to piv-1 and 2 was demonstrated. the highest prevalence and incidence of hi-antibodies was found for piv-3, followed by piv-1. there may have been a cross-reaction between piv-1 and -3 in group 1 calves. the low level of hi antibodies to piv-1 and 2 may have resulted fr ... | 1985 | 2986256 |
| intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides. | the intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35s]methionine-labeled cell extracts under reducing conditions. all of the virion structural proteins were detected in cell extracts, including: l, 180,000 molecular weight (180k); p, 83k; hn, 69k; np, 66k; f0, 60k; f1, 51k; and m, 38k. p and np were phosphorylated. hn and f were glycosylated. the kinetics of intracellular viral protein synthesis did not d ... | 1985 | 2987519 |
| impaired function of bovine alveolar macrophages infected with parainfluenza-3 virus. | bovine alveolar macrophages (bam) were harvested from nonsedated cattle, adhered to glass or plastic surfaces, and infected with parainfluenza-3 (pi-3) virus at a multiplicity of infection of 10. control and pi-3 virus-infected bam were compared at 24-hour intervals up to 168 hours for their ability to phagocytize antibody-coated sheep erythrocytes (eac) and latex particles, to kill staphylococcus epidermidis, and to alter intracellular acid phosphatase concentrations. the effect of antiviral se ... | 1985 | 2994528 |
| paramyxovirus antigens in osteoclasts from paget's bone tissue detected by monoclonal antibodies. | the fluorescent antibody technique using both monoclonal and specific polyclonal virus antibodies was applied to investigate the nature of the inclusions seen in the abnormal osteoclasts associated with paget's bone disease. the results show that antigens of measles virus, simian virus 5 (sv5) and human parainfluenza virus type 3 (pf3) could be detected in the osteoclasts but not in control bone cells. measles and sv5 nucleoprotein (np) and haemagglutinin-neuraminidase (hn) antigens were apparen ... | 1985 | 2995550 |
| synthesis and biological activity of certain 6-substituted and 2,6-disubstituted 2'-deoxytubercidins prepared via the stereospecific sodium salt glycosylation procedure. | a number of 6-substituted and 2,6-disubstituted pyrrolo[2,3-d]pyrimidine 2'-deoxyribonucleosides were prepared by the direct stereospecific sodium salt glycosylation procedure. reaction of the sodium salt of 4-chloro-6-methyl-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6a) or 4,6-dichloro-2-(methylthio)pyrrolo[2,3-d]pyrimidine (6b) with 1-chloro-2-deoxy-3,5-di-o-p-toluoyl-alpha-d-erythro-pentofuranose (9) provided the corresponding n7 2'-deoxy-beta-d-ribofuranosyl blocked derivatives (8a and 8c) whi ... | 1985 | 2995666 |
| synthesis and antitumor and antiviral activities of a series of 1-beta-d-ribofuranosyl-5-halocytosine (5-halocytidine) cyclic 3',5'-monophosphates. | a series of 1-beta-ribofuranosyl-5-halocytosine cyclic 3',5'-monophosphates (1-4) has been prepared. direct halogenation of cytidine 3',5'-monophosphate (ccmp) yielded the cl, br, and i compounds while 5-f-ccmp (1) was obtained on cyclization of the 5'-monophosphate. on in vitro testing of 1-4 against l1210 and p388 leukemias, only 1 showed significant low-level activity (id50 = 3.1 x 10(-4) mmol/l). derivatives 2-4 were inactive at 10(-1) mmol/l and also proved to have low viral ratings against ... | 1985 | 2984417 |
| comparison of interferon production in cattle after intranasal infection with parainfluenza-3 live vaccine and avirulent ibr/ipv-herpesvirus. | 1985 | 2996264 | |
| microwave sterilization of nitrous oxide nasal hoods contaminated with virus. | although there exists a desire to eliminate the possibility of cross-infection from microbial contaminated nitrous oxide nasal hoods, effective and practical methods of sterilization in a dental office are unsatisfactory. microwaves have been used to sterilize certain contaminated dental instruments without damage. in this study nasal hoods contaminated with rhinovirus, parainfluenza virus, adenovirus, and herpes simplex virus were sterilized in a modified microwave oven. ninety-five percent of ... | 1985 | 2999676 |
| protection from parainfluenza-3 virus and persistence of infectious bovine rhinotracheitis virus in sheep vaccinated with a modified live ibr-pi-3 vaccine. | ewes (n = 7) and their lambs (n = 12) were vaccinated with a commercial modified live infectious bovine rhinotracheitis-parainfluenza type 3 virus vaccine. both the vaccinated ewes and lambs and a group of unvaccinated ewes (n = 8) and their lambs (n = 13) were subsequently challenged with virulent parainfluenza type 3 virus. although absolute immunity to infection and clinical response was not conferred, the clinical response was less severe in vaccinated lambs. vaccinated animals also shed par ... | 1985 | 2985216 |
| prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv), bovine virus diarrhoea, bovine respiratory syncytial parainfluenza 3, adeno a and adeno b viruses in indigenous and imported moroccan cattle. | a serological survey on prevalence of antibodies to bovid herpesvirus 1 (ibr-ipv) bovine virus diarrhoea, bovine respiratory syncytial, parainfluenza 3, adeno a and b viruses was performed in 524 cattle from different areas and management conditions in morocco. general antibody prevalence was 62.8, 48.5, 70.4, 68.1, 9.0 and 12.4, for the six viruses, respectively. higher prevalence and high antibody titers to most of the investigated viruses were found among cattle from extensive management syst ... | 1985 | 2998260 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| human parainfluenza virus 3: purification and characterization of subviral components, viral proteins and viral rna. | a simple method was established that allowed large quantities of human parainfluenza 3 (pf3) virions to be isolated from tissue culture cells. the purity of the virus was sufficient for biochemical analysis of virion proteins. the density of pf3 virions was 1.18-1.20. purified virions contained seven viral proteins with estimated molecular weights of: l, 180 000; p, 83 000; hn, 69 000; np, 66 000; f0, 60 000; f1, 51 000; and m, 38 000, as determined by sodium dodecyl sulfate-polyacrylamide gel e ... | 1985 | 3000105 |
| epizootiological survey of parainfluenza-3, reovirus-3, respiratory syncytial and infectious bovine rhinotracheitis viral antibodies in sheep and goat flocks in quebec. | a serological survey was conducted in an attempt to detect antibodies against bovine respiratory viruses in sheep and goats from seven geographical areas of quebec. sera from 10% of the animals in 182 sheep flocks and 40 goat flocks were collected and specific antibodies against parainfluenza-3, reovirus type 3, respiratory syncytial and infectious bovine rhinotracheitis viruses were detected by hemagglutination-inhibition tests for the former viruses and complement fixation and seroneutralizati ... | 1985 | 3000551 |
| a simple method for increased recovery of purified paramyxovirus virions. | a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ... | 1985 | 3001119 |
| production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence. | monoclonal antibodies were produced against parainfluenza virus type 3 (pi-3) and used to identify pi-3 clinical isolates in cell culture and pi-3 antigen in cells obtained from nasopharyngeal (np) washes of patients. two (2e9 and 4g5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton pi-3 protein, and one antibody (4e5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. the three monoclonal antibodies did not cross-react by in ... | 1985 | 3001131 |
| a survey of respiratory syncytial virus and parainfluenza virus type 3 neutralising and immunoprecipitating antibodies in relation to paget disease. | the aetiology of paget disease of bone has not been established but certain features have suggested involvement of a parainfluenzalike virus. to seek further evidence of the possible role of paramyxoviruses in paget disease we have surveyed the presence of neutralising and immunoprecipitating antibodies to both respiratory syncytial virus and parainfluenza virus type 3 in the sera of patients attending a bone disease clinic. these two viruses were implicated by the sporadic observation of viral ... | 1985 | 3001223 |
| [studies on the pathogenesis of subacute thyroiditis]. | despite the fact that subacute thyroiditis (sat) is a common disease, its pathogenesis is still uncertain. at present, viral infection is simply thought to be a possible pathogenic factor. in caucasians and chinese, however, a strong association has recently been found between hla-bw35 and sat. therefore, genetic factors are also thought to participate in the onset of sat. in this study we have further attempted to clarify the pathogenesis of this disease in this regard. protocol: hla typing for ... | 1985 | 2998895 |
| persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83. | virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ... | 1985 | 3000066 |
| application of computer-assisted image analysis for studying viral structures. | the application of caie has been shown to be useful for analyzing the structures of rna viruses. critical assessment of this method is essential for the selection of the micrographs of viruses. in our experience this procedure was helpful for resolving some questions concerning virus morphology. | 1985 | 3006847 |
| antiviral effects of bovine interferons on bovine respiratory tract viruses. | the antiviral effects of bovine interferons on the replication of bovine respiratory tract viruses were studied. bovine turbinate monolayer cultures were treated with bovine interferons and challenged with several bovine herpesvirus 1 strains, bovine viral diarrhea virus, parainfluenza type 3 virus, goat respiratory syncytial virus, bovine respiratory syncytial virus, bovine adenovirus type 7, or vesicular stomatitis virus. treatment with bovine interferons reduced viral yield for each of these ... | 1984 | 6201505 |
| survey for antibodies to respiratory viruses in two groups of sheep in northern ireland. | two hundred serum samples from texel and texel crossbred sheep (non-indigenous breeds) and 200 from indigenous northern ireland breeds (mainly blackface, cheviot and border leicester crosses) were tested for antibodies to parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, bovine adenovirus (subgroups 1 and 2), influenza type a, maedi-visna virus and bovine virus diarrhoea virus. the percentage of animals with antibodies to parainfluenza virus 3 (50 to 56 per cent) and adenovirus ... | 1984 | 6095511 |
| serological studies of parainfluenza type 3 virus, bovine adenovirus type 3 and bovine respiratory syncytial virus infection in beef calves. | six serum samples were taken at monthly intervals from birth to weaning from each of 41 newborn calves in the autumn and spring calf crops of a beef cow--calf herd. the serum hemagglutination-inhibition (hi) antibody titres to parainfluenza type 3 virus (piv-3), virus-neutralization (vn) antibody titres to bovine adenovirus type 3 (bav-3) and bovine respiratory syncytial virus (brsv) were determined using microtitration techniques. there was serological evidence of a significantly higher inciden ... | 1984 | 6095517 |
| predisposition to invasive pneumococcal illness following parainfluenza type 3 virus infection in chimpanzees. | an outbreak of invasive disease, including pneumococcal bacteremia, meningitis, and pneumonia, involved 17 of 83 (20.5%) chimpanzees at a primate rehabilitation unit. invasive disease was more common in splenectomized than in nonsplenectomized animals (42.9% vs 18.4%), but the difference was not statistically significant. the outbreak followed closely an outbreak of upper respiratory tract infection (urti) that occurred with equal frequency in splenectomized and nonsplenectomized chimpanzees. th ... | 1984 | 6096328 |
| [pulmonary infections in pediatrics]. | 1984 | 6097981 | |
| serological diagnosis of parainfluenza virus infections by enzyme immunoassay with special emphasis on purity of viral antigens. | enzyme immunoassay (eia) for parainfluenza virus type 1, 2, and 3 antibodies was compared with the complement fixation test (cf) as a diagnostic method in 180 patients with respiratory symptoms. the cf test detected rises in parainfluenza virus antibodies in 30 cases, whereas eia detected 47 rises. patients with antibody rises in parainfluenza or mumps virus antibodies were studied for cross-reactions by cf, hemagglutination inhibition (hai), and eia using purified viral envelope glycoprotein an ... | 1984 | 6092530 |
| synthesis and antiviral/antitumor activities of certain pyrazolo[3,4-d]pyrimidine-4(5h)-selone nucleosides and related compounds. | several pyrazolo[3,4-d]pyrimidine-4(5h)-selone ribonucleosides were prepared as potential antiparasitic agents. treatment of 4-chloro-1-(2,3,5-tri-o-acetyl-beta-d-ribofuranosyl)pyrazolo [3,4-d]pyrimidine (5a) with selenourea and subsequent deacetylation gave 1-beta-d-ribofuranosylpyrazolo[3,4-d] pyrimidine-4(5h)-selone (6a). a similar treatment of 3-bromo-4-chloro-1-(2,3,5-tri-o-benzoyl-beta-d-ribofuranosyl)pyrazolo [3,4-d]pyrimidine (5b) with selenurea, followed by debenzoylation, gave the 3-br ... | 1984 | 6086922 |
| a glucose oxidase immunoenzyme stain for the detection of viral antigen or antibody on nitrocellulose transfer blots. | separation of mixtures of proteins by polyacrylamide gel electrophoresis followed by transfer of the proteins to support media such as nitrocellulose and detection by immunologic procedures provides a powerful analytic tool for assaying the antibody specificity of antisera or for following the purification of antigens. this technique requires fewer assumptions about antigenic solubility or antibody reactivity than immunoprecipitation methods. we present a glucose oxidase immunoenzyme staining pr ... | 1984 | 6205093 |
| resolution of two surface glycoproteins from human parainfluenza-3 virus by crossed immunoelectrophoresis. | the technique of two-dimensional crossed immunoelectrophoresis (cie) was used to resolve two glycoproteins from purified human parainfluenza type 3 virus. virus preparations were extracted with triton x-100 and fractionated by centrifugation in a beckman airfuge. two immunoprecipitates were detected by cie in the supernatant fractions, but were not found in the pellets from extracted virus. viral glycoproteins labeled with [35s]methionine were isolated by affinity chromatography on concanavalin ... | 1984 | 6088566 |
| association of parainfluenza-3 virus with bovine macrophages and blood cells: an in vitro study. | bovine blood cells and peritoneal and lung macrophages were exposed in vitro to parainfluenza-3 (pi-3) virus. residual nonadsorbed pi-3 virus (expressed in percentage of input virus) in the supernate of the various cell fractions 1 hour after incubation at 37 c was as follows: lung macrophages, 11%; peritoneal macrophages, 59%; monocytes, 26%; rbc, 14%; lymphocytes, 28%; and polymorphonuclear cells (pmn), 63%. lung macrophages, monocytes, lymphocytes, and pmn were monitored over a 72-hour period ... | 1984 | 6324623 |
| a simultaneous outbreak of respiratory syncytial virus and parainfluenza virus type 3 in a newborn nursery. | over a 3-week period, 20 of 34 (59%) infants in a newborn nursery developed nosocomial viral respiratory tract disease. either respiratory syncytial virus (seven infants) or parainfluenza virus type 3 (five) or both (two) were demonstrated in respiratory secretions from 14 of the 20 symptomatic patients. symptoms in the 20 infants included rhinitis (15 infants), cough (14), apnea (eight), pulmonary infiltrates (seven), and fever (six). there were no differences in symptoms between children infec ... | 1984 | 6325655 |
| brs virus, pi3 virus and bhv1 infections of young stock on self-contained dairy farms: epidemiological and clinical findings. | the role of bovine respiratory syncytial virus, parainfluenza type 3 virus and bovine herpesvirus 1 as disease agents in 28 groups of young cattle on 19 dairy farms which raised their own replacements was investigated. bovine respiratory syncytial virus infections occurred in 27, parainfluenza type 3 virus infections in all and bovine herpesvirus 1 infections in three of the 28 groups. some infections were accompanied by clinical signs while others were entirely subclinical. clinical respiratory ... | 1984 | 6326374 |
| production of cattle immunotolerant to bovine viral diarrhea virus. | inoculation of bovine virus diarrhea virus into 58 to 125 day old fetuses of bovine virus diarrhea virus seropositive pregnant cows, or inoculation of bovine virus diarrhea virus into seronegative cows 42 to 114 days pregnant, may produce clinically normal calves which are persistently infected with the specific isolate of bovine virus diarrhea virus yet seronegative to the homologous and heterologous isolates. reinoculation of these persistently infected cattle with their homologous isolate pro ... | 1984 | 6326980 |
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| evaluation of cold-adapted and temperature-sensitive mutants of parainfluenza virus type 3 in weanling hamsters. | cold-adapted (ca) and temperature-sensitive (ts) mutants of parainfluenza virus 3 were produced by serially passaging wild type (wt) parainfluenza virus 3 at 20 degrees c 45 times. previously, plaque-purified viruses (clones) were selected from the wt parent and from cold passage levels 7, 12, 18, and 45 and characterized in vitro. in the present study, we evaluated at least one mutant from each cold passage level for attenuation in hamsters. four of the five mutants tested were ts and ca (one e ... | 1984 | 6327899 |
| prevalence of bovine herpesvirus-1, bovine viral diarrhea, parainfluenza-3, goat respiratory syncytial, bovine leukemia, and bluetongue viral antibodies in sheep. | sera from healthy sheep were collected in january and march 1982 from flocks of sheep located in southwestern and southeastern louisiana. these sera were tested for bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv), parainfluenza-3 (pi-3) virus, and goat respiratory syncytial virus (grsv) antibodies by microtitration virus-neutralization test. the sera were tested also for bovine leukemia virus (blv) and bluetongue virus (btv) antibodies by immunodiffusion tests. the number of flo ... | 1984 | 6329044 |
| antiviral activity of c-5 substituted tubercidin analogues. | the pyrrolo[2,3-d]pyrimidine nucleoside antibiotics tubercidin, toyocamycin, and sangivamycin and the synthetic analogues 5-chloro-, 5,6-dichloro-, 5-bromo-, 6-bromo-, 5,6-dibromo-, 5-iodo-, 5-(1-hydroxyethyl)-, 5-(1-methoxyethyl)-, (e)-5-(2-bromoethenyl)-, (e)-5-(2-cyanoethenyl)-, 5-(2-buten-1-yl)-, 5-(3-hydroxypropyl)-, and 5-butyltubercidin were evaluated for their antiviral properties against six rna viruses and three dna viruses in hela cell, primary rabbit kidney cell, and vero cell cultur ... | 1984 | 6699874 |
| antibody and complement-mediated cytotoxicity for bovine parainfluenza-3 virus-infected cells. | an antibody-complement-mediated cytotoxicity assay was employed to determine whether susceptible host cells infected with bovine parainfluenza-3 virus could be destroyed by the humoral immune mechanism. the cytotoxicity assay yielded 21.5% to 76.9% specific release of 51cr with sera collected from calves 28 days after inoculation with the virus. a multiplicity of infection of 5, a postinfection period of 2 days, an antibody dilution of 1:10, and an incubation period of 12 hours with antibody and ... | 1984 | 6331235 |
| viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different european countries. | from november 1978 to october 1981, a total of 7716 specimens of nasopharyngeal secretions were examined by the rapid immunofluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (rsv), influenza virus a, and parainfluenza viruses 1 and 3. the tests were carried out in six different virus laboratories located in newcastle upon tyne (england), copenhagen, oslo, stockholm, turku (finland), and vienna; laboratories in lisbon and paris participated ... | 1984 | 6375886 |
| broad-spectrum synergistic antiviral activity of selenazofurin and ribavirin. | the antiviral effects of selenazofurin (2-beta-d-ribofuranosylselenazole-4-carboxamide, selenazole), ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), and 3-deazaguanosine (6-amino-1-beta-d-ribofuranosylimidazo-[4.5-c]pyridin-4(5h)-one) were investigated separately and in various combinations in an in vitro study. the combination interactions were evaluated at seven drug concentrations, graphically (isobolograms) or by using fractional inhibitory concentration indices against mump ... | 1984 | 6517540 |
| cell mediated immunity of calves to bovine parainfluenza-3 virus; leukocyte migration inhibition and leukocyte aggregation response. | 1984 | 6328797 | |
| susceptibility of specific pathogen-free lambs to concentrations of pasteurella haemolytica serotype a2 in aerosols. | a 100-fold reduction in the numbers of organisms in an aerosol of pasteurella haemolytica used to infect specific pathogen-free lambs did not alter the number of cases of pneumonia which resulted. in a separate experiment a further 10-fold reduction in the number of organisms in the aerosol did not cause fewer cases of pneumonia. | 1984 | 6097970 |
| synthesis and antiviral/antitumor activities of certain 3-deazaguanine nucleosides and nucleotides. | a new procedure for the preparation of the antiviral and antitumor agent 3-deazaguanine (1) and its metabolite 3-deazaguanosine (2) has been developed by reacting methyl 5(4)-(cyanomethyl) imidazole-4(5)-carboxylate (4) and 5-(cyanomethyl)-1- (2,3,5-tri-o-benzoyl-beta-d-ribofuranosyl)imidazole-4-carboxylate (6), respectively, with hydrazine. the 3-deazaguanosine 3',5'-cyclic phosphate (13) was prepared from 5-(cyanomethyl)-1-beta-d-ribofuranosyl-imidazole-4-carboxamide 5'-phosphate. glycosylatio ... | 1984 | 6092634 |
| structural characterization of virion proteins and genomic rna of human parainfluenza virus 3. | the virion proteins and genomic rna of human parainfluenza virus 3 have been characterized. the virion contains seven major and two minor proteins. three proteins of 195 x 10(3) molecular weight (195k), 87k, and 67k are associated with the nucleocapsid of the virion and have been designated l, p, and np, respectively. three proteins can be labeled with [14c]glucosamine and have molecular weights of 69k, 60k, and 46k. we have designated these proteins as hn, f0, and f1, respectively. hn protein h ... | 1984 | 6092708 |
| acquisition of serum antibodies to specific viral glycoproteins of parainfluenza virus 3 in children. | a radioimmunoprecipitation assay was used to study antibody responses to parainfluenza virus 3 glycoproteins in human sera. the method was not only more sensitive than the neutralization test for the detection of antibody but also provided semiquantitative assessments of the antibody response to both glycoproteins in a single assay system. anti-hemagglutinin-neuraminidase titers were consistently higher than anti-fusion levels in the same serum specimen. thirteen children were monitored serologi ... | 1984 | 6092715 |
| isolation of avian paramyxovirus 2 and 3 from turkeys in brittany. | 1984 | 6087542 | |
| effect of anti-prostaglandin therapy in experimental parainfluenza type 3 pneumonia in weaned, conventional calves. | an acute pneumonia was induced experimentally in 10, 10- to 12-week-old conventional calves by administration into the upper airways of a pathogenic strain of parainfluenza type 3 (pi3) virus. the experimental calves had been selected on the basis of freedom from clinical evidence of respiratory and other diseases, freedom from current infection by pi3 virus as judged by repeated nasopharyngeal swabbing and freedom from earlier pi3 virus infection as judged by their lack of significant levels of ... | 1984 | 6089403 |
| prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec. | serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ... | 1984 | 6089626 |
| parainfluenza virus type 3: seasonality and risk of infection and reinfection in young children. | in houston the temporal occurrence of infections with parainfluenza virus type 3 has evolved from an endemic to an epidemic pattern. continuous virological surveillance for six years demonstrated that most infections occurred the late winter or spring after influenza virus activity. at least two-thirds of children observed in the houston family study were infected with this virus in each of the first two years of life, and the risk of illness was about 30/100 children per year. after two years o ... | 1984 | 6094674 |
| prevention of bovine respiratory syncytial virus infection and clinical disease by vaccination. | a double blind field trial was carried out with a live attenuated bovine respiratory syncytial virus vaccine. the trial involved 530 calves, two to 10 months old, on 27 dairy farms, where respiratory problems due to bovine respiratory syncytial virus infections had been observed during the preceding year. in 17 herds either all calves were vaccinated (nine groups) or all calves received a placebo (eight groups). in 10 herds half the number of calves were vaccinated and the other half kept as non ... | 1984 | 6097014 |
| [nosocomial epidemic episode of acute respiratory disease due to para-influenza virus type 3]. | 1984 | 6100623 | |
| a simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses involving adsorption to erythrocytes. | a simple procedure for the analysis of the structural proteins of influenza and parainfluenza viruses utilizing adsorption to erythrocytes is described. the method involves virus growth in the presence of [35s]methionine, adsorption of clarified culture medium with a 0.5% suspension of either guinea-pig or chicken erythrocytes and analysis of the virus-erythrocyte aggregates by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). all of the structural proteins can be detected u ... | 1984 | 6323512 |
| the prevalence and microbiology of pneumonia in a flock of lambs. | a flock of new zealand romney lambs on a property in hawkes bay was examined from august 1978 to june 1979. in november, the lambs in the flock were allocated to groups as follows: 600 lambs from which groups were selected for slaughter by commercial criteria each month (selected groups); 700 lambs which were subdivided into 8 groups (random groups), one of which was sent for slaughter each month; and 25 lambs to monitor serological responses to viral infections (surveillance group). at slaughte ... | 1983 | 16030927 |
| vaccination against ovine pneumonia: a progress report. | the results of three field trials of a live, cold-adapted, parainfluenza virus type 3 vaccine supported the hypothesis that infection with this virus initiates some ovine pneumonias. the serological response following vaccination was poor and only 22-44% of vaccinates developed a serum antibody response by two weeks after vaccination. nevertheless, the prevalence of pneumonia was lower in vaccinated lambs in all three trials at the time that natural infection with pi3 was detected in the non-vac ... | 1983 | 16030967 |
| monoclonal antibodies to epitopes shared by actin and vimentin obtained by paramyxovirus immunization. | three hybridoma clones producing igm antibodies against actin were obtained from mice immunized with purified virions of paramyxoviruses. when tested on growing lung fibroblasts, ascites fluids of all clones stained in immunofluorescence cytoplasmic bundles of microfilaments, but also fibrillar networks. on colchicine-treated cells, perinuclear coils were seen in addition to microfilament bundles. in addition, one clone gave a pronounced speckled staining to the nuclei. absorption of the ascites ... | 1983 | 6190666 |
| ribavirin treatment of viral pneumonitis in severe combined immunodeficiency disease. | 1983 | 6136856 | |
| reactivation of bovid herpesvirus 1 and 2 and parainfluenza-3 virus in calves latently infected. | two experiments were carried out to determine whether bovid herpesvirus (bhv) 2 is able to induce a recurrent infection in experimentally infected calves. in the first experiment the stress induced by dexamethasone (dms) treatment failed to reactivate the clinical condition or to induce shedding of bhv2. however, treatment with dms reactivated a latent bhv1 infection in all calves previously inoculated with bhv2 and also in two noninoculated controls. probably, because of the interference by bhv ... | 1983 | 6313287 |
| prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in mexico. | 1983 | 6316006 | |
| improvement of respiratory syncytial virus replication in actively growing hep-2 cells. | the growth of respiratory syncytial (rs) and parainfluenza type iii (pi3) viruses has been studied in actively growing versus relatively stationary hep-2 cells. there was no effect on pi3 virus growth. rs virus synthesis was stimulated from 20- to 60-fold by growth in actively growing cultures when the cell density was approximately 1/3 that of a confluent culture. this stimulation was manifested by a greater yield of virus per cell, more virus-specific mrna produced per cell, and a 50% decrease ... | 1983 | 6317708 |
| persistence of antibodies and anamnestic response in calves vaccinated with inactivated infectious bovine rhinotracheitis virus and parainfluenza-3 virus vaccines. | persistence of antibodies in calves vaccinated with 2 types of inactivated infectious bovine rhinotracheitis (ibr) virus and parainfluenza-3 (pi-3) virus vaccines were determined. calves seronegative for ibr and pi-3 viruses were inoculated with 2 doses of inactivated ibr virus-pi-3 virus vaccines administered 2 weeks apart. blood samples were obtained from the calves for serum at 2 weeks, 6 months, and 1 year after vaccination. the serums were tested by serum-neutralization tests. antibody resp ... | 1983 | 6303996 |
| exposure of calves to aerosols of parainfluenza-3 virus and pasteurella haemolytica. | the present study was undertaken to investigate whether sequential exposure to aerosols of parainfluenza-3 virus followed by pasteurella haemolytica, or p. haemolytica followed by parainfluenza-3 virus, could lead to the production of pulmonary lesions in conventionally-raised calves. twenty male calves with low serum antibody titres to both organisms were placed in five equal groups. synergism of parainfluenza-3 virus and p. haemolytica was not demonstrated in any of the sequentially infected g ... | 1983 | 6320999 |
| disseminated parainfluenza infection in a child with severe combined immunodeficiency. | an 8-month-old male infant with severe combined immunodeficiency syndrome was admitted to the hospital with pneumonitis and, subsequently, died at 21 months of age. during his lengthy hospitalization, parainfluenza virus type 3 (piv-3) was isolated from respiratory secretions and unusual sites, such as pericardial fluid, csf, and wbcs. a postmortem examination showed apparent viral pancreatitis, and piv-3 was isolated from the lung, brain, and pericardial fluid. to our knowledge, this is the fir ... | 1983 | 6314807 |
| serology of bovine parainfluenza virus type 3: comparison of the enzyme linked immunosorbent assay and hemagglutination inhibition. | an enzyme linked immunosorbent assay (elisa) for the detection of antibody to bovine parainfluenza virus type 3 has been compared with the hemagglutination inhibition test on 130 field sera, and seven other paired sera showing a significant raise of titers. the elisa was found to be four to 64 times more sensitive than the hemagglutination inhibition test and the two tests demonstrated a good correlation. | 1983 | 6309348 |
| ultrastructural features of experimental parainfluenza type 3 virus pneumonia in calves. | ultrastructural changes observed in the acute and repair stages of experimental p13 virus pneumonia in young calves are described. in the early acute stage of the pneumonia, paramyxovirus replication was observed within epithelial cells of the respiratory tract and within alveolar macrophages. there was loss of cilia and of ciliated cells in small bronchi and bronchioli. proliferation of non-ciliated bronchiolar cells (clara cells) produced zones of hyperplastic bronchiolar epithelium and, withi ... | 1983 | 6309920 |
| effects of bovine parainfluenza-3 virus on phagocytosis and phagosome-lysosome fusion of cultured bovine alveolar macrophages. | bovine parainfluenza-3 virus (pi-3v) replicated in cultured bovine alveolar macrophages (bam) in vitro. cytopathic changes included spindle cell and giant cell formation, diffuse cell lysis, and cellular detachment progressing to total destruction of the monolayer. direct immunofluorescent microscopy revealed viral antigen in greater than 90% of bam on post-inoculation day (pid) 3. virus titers in culture supernatants increased by a factor of 1.6 x 10(6) tcid50 by pid 6. candida glabrata was pha ... | 1983 | 6314852 |
| neuraminidase activity and syncytial formation in variants of parainfluenza 3 virus. | by a sensitive fluorometric assay method, we could definitely demonstrate neuraminidase activity for two variants of parainfluenza 3 virus, m and sc, which were previously shown to have no detectable neuraminidase activity. the enzyme activities of these viruses were very similar to each other, showing a much lower catalytic rate, a much higher km value, and a more acidic ph optimum than those of the virus variants of high neuraminidase activity, 910n, lt, and mr. m and sc viruses eluted from gu ... | 1983 | 6307881 |
| broad-spectrum antiviral activity of 2-beta-d-ribofuranosylselenazole-4-carboxamide, a new antiviral agent. | the relative in vitro antiviral activities of three related nucleoside carboxamides, ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide), and selenazole (2-beta-d-ribofuranosylselenazole-4-carboxamide), were studied against selected dna and rna viruses. although the activity of selenazole against different viruses varied, it was significantly more potent than ribavirin and tiazofurin against all tested representatives of the ... | 1983 | 6615611 |
| variation of virus replication in primary bovine kidney cell cultures derived from 68 three-day-old calves. | primary kidney cell cultures were prepared from 68 three-day-old calves. complete monolayers of these cultures were infected separately with viral diarrhea, infectious bovine rhinotracheitis, parainfluenza, and adenovirus 7 viruses. the yield of virus from all infected cultures was calculated by plaque titer assay after 2 to 4 days' incubation. the variation of virus yield was substantial between individual cultures. | 1983 | 6318615 |
| lack of virus-specific bacterial adherence to bovine embryonic lung cells infected with bovine parainfluenza virus type 3. | infection of bovine embryonic lung cells with bovine parainfluenza virus type 3 did not induce in vitro, virus-specific, hemadsorption-related adherence of corynebacterium pyogenes, haemophilus somnus, staphylococcus aureus, streptococcus zooepidemicus, pasteurella haemolytica, listeria monocytogenes, escherichia coli, pasteurella multocida, brucella sp., or salmonella typhimurium. | 1983 | 6302133 |
| characteristics of the polyriboinosinic acid:polyribocytidylic acid assay for noncytopathogenic bovine viral diarrhea virus. | the use of polyriboinosinic acid:polyribocytidylic acid (poly i:c) for noncytopathogenic bovine viral diarrhea virus (nc-bvdv) assay (pinba) was studied. several viruses were tested for their suitability as test challenge viruses. in addition to vesicular stomatitis virus, which previously was shown to be a suitable challenge virus, bovine enteroviruses also were found to be suitable, whereas infectious bovine rhinotracheitis virus and parainfluenza type 3 virus were only marginally suitable. bo ... | 1983 | 6314854 |
| a field trial, of preshipment vaccination, with intranasal infectious bovine rhinotracheitis-parainfluenza-3 vaccines. | a total of 849 calves, 278 controls, 335 vaccinated intranasally with ibr-pi3/ts and 236 vaccinated intranasally with ibr-pi3/ptc were studied in a field trial of preimmunization. all calves were vaccinated in saskatchewan at least three weeks prior to shipment to feedlots. four hundred and twenty six calves were not sold within eight weeks of vaccination; however, seven of these died within four weeks of vaccination. treatment rates varied from 1.0% to 5.2%. there was no significant effect of v ... | 1983 | 6315194 |
| the relationship of serology and nasal microbiology to pulmonary lesions in feedlot cattle. | a group of 62 beef calves, born and raised in an institutional herd, were transferred at nine months of age to a commercial feedlot where they remained until slaughter seven months later. clinical, immunological and microbiological monitoring was carried out during this period. no serious clinical illness occurred. one hundred percent seroconversion to bovine virus diarrhea virus took place after introduction of the calves into the feedlot as well as almost complete (59/62) seroconversion to bov ... | 1983 | 6315201 |
| epizootic of parainfluenza-3 virus infection in gibbons. | a laboratory-housed breeding colony of white-handed gibbons (hylobates lar) experienced an epizootic of upper respiratory tract disease characterized by lethargy, anorexia, coughing, and serous rhinorrhea. signs were more severe in adults than in offspring, and all animals recovered without complications. base-line, acute, and convalescent sera from the most severely affected gibbons were tested for antibodies against a wide spectrum of infectious agents. for personnel known to have had contact ... | 1983 | 6315655 |
| antibodies against viral pathogens in dairy cattle in colombia. | 1983 | 6316593 | |
| experimental parainfluenza type 3 infection in young lambs: clinical, microbiological, and serological response. | five, 1-week-old, colostrum-deprived lambs were inoculated transtracheally with a parainfluenza type-3 (pi-3) virus that had been isolated from a pneumonic lamb lung. a biphasic febrile response, cough, rapid breathing followed by forced expirations, listlessness, and anorexia were observed in the lambs. there were multifocal areas of consolidation in the lungs of all lambs and ulcerations in the nasal mucosa of three lambs. serum antibody titers to pi-3 virus ranged from 2 to 16 in lambs necrop ... | 1983 | 6316628 |
| electron microscopic evidence for parainfluenza type iii/retrovirus phenotypically mixed particles. | sublines of malignant permanent human fibroblast cell lines were shown to be infected by a type-d retrovirus (pmfv) and by a parainfluenza virus type iii in an earlier study (2, 6). careful electron microscopical investigations of these sublines have proved "viral structural elements" of both viruses in the same cell. we obtained electron microscopic evidence--though rarely--for parainfluenza type iii/pmfv mixed-particles. | 1983 | 6305302 |
| protein subunit vaccines of parainfluenza type 3 virus: immunogenic effect in lambs and mice. | protein subunit vaccines were prepared from a mixture of the haemagglutinin (hn) and fusion (f) glycoproteins of parainfluenza type 3 virus (pi-3). the glycoproteins were isolated in three different forms and characterized by their sedimentation coefficients: 30s protein micelles (a complex of several hn and f glycoproteins devoid of detergent and lipid), 18s protein-tx complexes (a complex of several glycoproteins containing the detergent triton x-100), and 4s protein-tx complexes (probably mon ... | 1983 | 6306152 |
| immunosuppression in goats inoculated with parainfluenza type 3 virus. | the humoral and cellular immune responses of goats experimentally infected with an ovine isolate of parainfluenza type 3 virus (pi-3) were examined. virus neutralization and enzyme-linked immunosorbent assays were used to determine antibody in the serum and csf. lymphocyte stimulation, measured by [3h]thymidine incorporation into peripheral blood leukocytes, was used to determine cellular responses to phytomitogens and virus. there were significant suppression of peripheral blood leukocyte respo ... | 1983 | 6318612 |
| evaluation of the attenuated vaccine para-ribovac. | 1983 | 6316694 | |
| inhibition by vasoactive intestinal peptide of antigen-induced histamine release from guinea-pig minced lung. | vasoactive intestinal peptide (vip) was found to inhibit ovalbumin-induced histamine release from guinea-pig minced lung. maximum inhibition occurred with a 10-20 min time of preincubation with vip. spontaneous histamine release was not altered by vip. with 2 x 10(-6) m vip, the dose-response curve to ovalbumin was shifted about 3-fold to the right, but the maximum histamine release was unaltered. respiratory tract infection with parainfluenza 3 virus did not influence the inhibitory effect of v ... | 1983 | 6189733 |
| tick-borne fever and concurrent parainfluenza-3 virus infection in sheep. | 1982 | 6292262 |