Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| invasion mechanisms among emerging food-borne protozoan parasites. | food-borne parasitic diseases, many known to be more prevalent in poor countries with deficient sanitary conditions, are becoming common worldwide. among the emerging protozoan parasites, the most prominent is trypanosoma cruzi, rarely reported in the past to be transmitted by the oral route but currently responsible for frequent outbreaks of acute cases of chagas disease contracted orally and characterized by high mortality. several other food-borne protozoans considered emerging include the ap ... | 2011 | 21840261 |
| novelties on amoebiasis: a neglected tropical disease. | in accordance with the 1997 documents of the world health organization (who), amoebiasis is defined as the infection by the protozoan parasite entamoeba histolytica with or without clinical manifestations. the only known natural host of e. histolytica is the human with the large intestine as major target organ. this parasite has a very simple life cycle in which the infective form is the cyst, considered a resistant form of parasite: the asymptomatic cyst passers and the intestinal amoebiasis pa ... | 2011 | 21731305 |
| calpains are involved in entamoeba histolytica-induced death of ht-29 colonic epithelial cells. | entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. e. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. in this study, we investigated whether calpains are involved in the e. histolytica-induced cell death of ht-29 colonic epithelial cells. when ht-29 cells were co-incubated with e. histolytica, th ... | 2011 | 21738275 |
| erythema nodosum-like lesions in a renal transplant recipient. | 2011 | 21690541 | |
| structure analysis of entamoeba histolytica enolase. | entamoeba histolytica enolase (eheno) reversibly interconverts 2-phosphoglyceric acid (2-pga) and phosphoenolpyruvic acid (pep). the crystal structure of the homodimeric eheno is presented at a resolution of 1.9ôçà+à. in the crystal structure eheno presents as an asymmetric dimer with one active site in the open conformation and the other active site in the closed conformation. interestingly, both active sites contain a copurified 2-pga molecule. while the 2-pga molecule in the closed active sit ... | 2011 | 21697600 |
| Intestinal Parasite Screening in Internationally Adopted Children: Importance of Multiple Stool Specimens. | Objective: Our goal was to determine the prevalence of intestinal parasites in internationally adopted children, to examine factors associated with infection, and to determine if evaluating multiple stool specimens increases the yield of parasite identification. Methods: We evaluated internationally adopted children with at least 1 stool specimen submitted for ova and parasite testing within 120 days after arrival to the United States. In children submitting 3 stool specimens, in which at least ... | 2011 | 21824880 |
| benzotriazoles and indazoles are scaffolds with biological activity against entamoeba histolytica. | parasitic infections caused by entamoeba histolytica are still major threats against public health, especially in developing countries. although current therapies exist, the problems associated with parasite resistance and negative side effects make it imperative to search for new therapeutic agents. a systematic scaffold analysis reported herein of a public database containing 474 antiamoebic compounds reveals that benzimidazole is the most active scaffold reported thus far. to gain insights in ... | 2011 | 21821786 |
| Rab7 and actin cytoskeleton participate during mobilization of +¦1EHFNR in fibronectin-stimulated Entamoeba histolyticatrophozoites. | In vitro interaction of Entamoeba histolytica trophozoites with fibronectin (FN) induces redistribution of the amoebic fibronectin receptor (+¦1EhFNR). Trafficking of beta1 integrins is important for cell adhesion and migration in higher eukaryotes and requires the participation of Rab proteins. In E. histolytica, the machinery involved in integrin trafficking is not completely known. EhRab7 is a 24.5-kDa protein whose alignment with other Rab7 proteins demonstrated that it shared significant ho ... | 2011 | 21823201 |
| study of parasitic infection among children of sukumbasi basti in kathmandu valley. | keeping in view of heavy burden of intestinal parasitosis, present study was done to find out the prevalence of intestinal parasitic infection in children (aged <16 years) of sukumbasi (people living without land ownership) basti (community) in kathmandu valley. a total of 279 stool samples collected in clean, dry and screw capped plastic container were firstly examined for the presence of adult worm and/or segments of worms. samples fixed in 10% formal-saline were then examined microscopically ... | 2011 | 21991692 |
| Prevalence and risk factors of helminths and intestinal protozoa infections among children from primary schools in western Tajikistan. | Intestinal parasitic infections represent a public health problem in Tajikistan, but epidemiological evidence is scarce. The present study aimed at assessing the extent of helminths and intestinal protozoa infections among children of 10 schools in four districts of Tajikistan, and to make recommendations for control. | 2011 | 21981979 |
| Synchronous tumor with diffuse large B-cell lymphoma and ameboma. | 2011 | 21947830 | |
| fatal amebic colitis after high-dose dexamethasone therapy for newly diagnosed multiple myeloma. | 2011 | 20467744 | |
| entamoeba histolytica and trichomonas vaginalis: trophozoite growth inhibition by metronidazole electro-transferred water. | the influence of low-frequency electromagnetic (lf-em) waves on microorganisms has been a subject of experimental investigations for more than two decades and the results are promising. in parallel, an interesting procedure known as biophysical-information-therapy or bioresonance therapy (brt) which in principle is based on lf-em stimulation, has emerged. brt was discovered in the late 1980's but it is still poorly studied. this paper demonstrates that by transferring metronidazole information t ... | 2011 | 20603119 |
| entamoeba invadens: identification of adf/cofilin and their expression analysis in relation to encystation and excystation. | the differentiation processes of excystation and encystation of entamoeba are essential for infection and completion of their life-cycle, and the processes need cell motility and its control by actin cytoskeletal reorganization. this study investigated actin depolymerizing factor (adf)/cofilin (cfl) family proteins, which are important molecules in actin cytoskeletal reorganization, in entamoeba invadens in relation to the encystation and excystation. axenic culture systems were used to induce e ... | 2011 | 20691182 |
| entamoeba invadens: dynamics of dna synthesis during differentiation from trophozoite to cyst. | the dna dynamics which mediate conversion of uni-nucleate trophozoite into quadrinucleate cyst in entamoeba histolytica is not well understood. here, we have addressed this question in entamoeba invadens (a model system for encystation) through a detailed time course study of the differentiation process. we combined flow cytometric analysis with the change in rate of thymidine incorporation and the number of nuclei per cell. our data shows that during encystment the cell population passes throug ... | 2011 | 20727884 |
| evidence for a novel entamoeba histolytica lectin activity that recognises carbohydrates present on ovalbumin. | entamoeba histolytica, an intestinal amoeba that causes dysentery and liver abscesses, acquires nutrients by engulfing bacteria in the colonic lumen and phagocytoses apoptotic cells during tissue invasion. in preliminary studies to identify ligands that stimulate amoebic phagocytosis, we used ovalbumin immobilized on latex particles as a potential negative control protein. surprisingly, ovalbumin strongly stimulated e. histolytica particle uptake. experiments using highly purified ovalbumin conf ... | 2011 | 20807536 |
| patterns of gastro-intestinal parasites and commensals as an index of population and ecosystem health: the case of sympatric western chimpanzees (pan troglodytes verus) and guinea baboons (papio hamadryas papio) at fongoli, senegal. | the exponential decline of great apes over the past 50 years has resulted in an urgent need for data to inform population viability assessment and conservation strategies. health monitoring of remaining ape populations is an important component of this process. in support of this effort, we examined endoparasitic and commensal prevalence and richness as proxies of population health for western chimpanzees (pan troglodytes verus) and sympatric guinea baboons (papio hamadryas papio) at fongoli, se ... | 2011 | 20853397 |
| acetaldehyde/alcohol dehydrogenase-2 (ehadh2) and clathrin are involved in internalization of human transferrin by entamoeba histolytica. | transferrin (tf) is a host glycoprotein capable of binding two ferric-iron ions to become holotransferrin (holotf), which transports iron in to all cells. entamoeba histolytica is a parasitic protozoan able to use holotf as a sole iron source in vitro. the mechanism by which this parasite scavenges iron from holotf is unknown. an e. histolytica holotf-binding protein (ehtfbp) was purified by using an anti-human transferrin receptor (tfr) monoclonal antibody. ehtfbp was identified by ms/ms analys ... | 2011 | 20847004 |
| frequency of entamoeba histolytica and entamoeba dispar prevalence among patients with gastrointestinal complaints in chelgerd city, southwest of iran(*). | differentiation between entamoeba histolytica and entamoeba dispar is very important for both clinical therapy and epidemiological studies. although these two species are morphologically identical, they have differences in genetic, chemical specifications and pathogenicity. this study was carried out to differentiate e. histolytica from e. dispar and also to find out frequency of the two species. | 2011 | 22973344 |
| high-level parasitic contamination of soil sampled in ibadan metropolis. | soil transmitted helminthes infections are common chronic human infections worldwide, this has been recognized as an important health problem, particularly in developing countries. the study was conducted within ibadan metropolis in oyo state, south western nigeria between september 2008 and march 2009 to determine the prevalence of intestinal parasite in soil samples within the city. a total of 102 soil samples were collected from different sources from five local government areas ranging from ... | 2011 | 22783681 |
| prevalence of intestinal parasites and bacteria among food handlers in a tertiary care hospital. | the aim of this work is to determine the prevalence of intestinal parasites and bacteria among the food handlers. | 2011 | 22529512 |
| status of intestinal parasitosis among hospital visiting patients in deukhury valley, dang, nepal. | intestinal parasitosis is highly prevalent among the general population in nepal. this study aimed to assess the status of intestinal parasitosis among patients attending deukhury community hospital, lamahi, dang, situated in the mid western region of nepal. a total of 210 stool samples examined by direct smear technique were reported. the overall prevalence of intestinal parasitic infection was found to be 21.4% (m = 23.5% vs f = 19.3%). children < 15 yrs age were infected more often as compare ... | 2011 | 22364091 |
| prevalence of intestinal parasites among patients of a tertiary hospital in benin city, nigeria. | intestinal parasitic infections have been described as constituting the greatest single worldwide cause of illness and disease. | 2011 | 22363084 |
| gastrointestinal parasites of captive and free-roaming primates at the afi mountain primate conservation area in calabar, nigeria and their zoonotic implications. | a study on the gastrointestinal parasites among free-living and captive primates at the afi mountain, primate conservation area in calabar, nigeria was undertaken for the first time to ascertain their zoonotic implications. faecal samples were subjected to direct smear, floatation, quantitative estimation of helminth eggs (epg) and oocysts (opg), larval isolation and identification by modified baerman's technique and oocyst sporulation for specie identification. out of the 108 primates examined, ... | 2011 | 22308652 |
| probing the antiamoebic and cytotoxicity potency of novel tetrazole and triazine derivatives. | a series of compounds bearing a tetrazole and triazine ring motif conjugated with a so(2)nh function were synthesized and investigated for their antiamoebic potency. cytotoxicity of the compounds was checked on human hepatocellular carcinoma cell line hepg2. incorporation of triazine ring in place of tetrazole resulted in a precipitous increase in the antiamoebic activity of the compounds. antiamoebic activity of the investigated compounds was found to be position and substituent dependent. in v ... | 2011 | 22236470 |
| intestinal parasitic infestation among children in a semi-urban indian population. | survey on the prevalence of various intestinal parasitic infestations in different geographic regions is a prerequisite to obtain an accurate understanding of the burden and cause of intestinal parasitic infestations in a particular area. the aim of the present study was to determine the intestinal parasitic infestation among children in a semi-urban area. | 2011 | 23508675 |
| a coprological survey for assessing intensity of parasitic infection in school children: cross-sectional study. | intestinal parasitic infections (ipis) are endemic worldwide and have been the cause of illness and disease worldwide. | 2011 | 23507633 |
| profile of catering staff at a tertiary care hospital in mumbai. | food borne illnesses, even today, continue to be a major public health problem in both developing and developed nations. food handlers play an important role in ensuring food safety throughout the chain of production, processing, storage and preparation.health of food handlers is of great importance for maintaining hygienic quality of food prepared and served by them. thus, the present study was conducted to study socio-demographic characteristics, morbidity pattern and immunization status of ca ... | 2011 | 23390464 |
| cloning of a 1.8 kb repeated sequence for the identification and comparison of giardia intestinalis isolates. | restriction enzyme digestion of bulk dna from giardia intestinalis reveals the presence of repeated sequences. a prominent 1.8 kb band in the alu i profile was cloned into the puc8 plasmid (pgi7) and used for comparing strains. when blots of dna of 34 isolates from different geographic areas are probed with pgi7, hybridization with identical intensities can be detected. however, some strains give different hybridization patterns with several restriction enzymes. no hybridization of pgi7 can be d ... | 2011 | 23195568 |
| Public health and clinical importance of amoebiasis in Malaysia: a review. | Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts of the world. Similarly Entamoeba moshkovskii, which was long considered to be a free-living amoeba is also morphologically identical to E. histolytica and E. dispar, and is highly p ... | 2011 | 22041740 |
| characterization of a novel entamoeba histolytica strain from burkina faso, africa, possessing a unique hexokinase-2 gene. | an entamoeba histolytica strain (bf-841 cl1) that originated from burkina faso, africa presented with novel, polymorphic genotypes of the serine-rich e. histolytica protein and the anodic hexokinase‑2 (hxk-2) isoenzyme band, which showed less electrophoretic mobility than that of an e. histolytica reference strain [hm-1:imss cl6 (zymodeme (z)-ii)] by starch gel electrophoresis and isoelectric focusing (ief). the hxk-2 gene of bf-841 cl1 had amino acid variations at four positions compared to the ... | 2011 | 22091458 |
| prevalence of intestinal parasites among patients of al-noor specialist hospital, makkah, saudi arabia. | to determine the prevalence of intestinal parasites among patients of a tertiary care hospital. | 2011 | 22043412 |
| High-resolution electron microscopical study of cyst walls of Entamoeba spp. | Knowledge of the fine structural organization, molecular composition and permeability properties of the cell surface of intestinal protozoan cysts is important to understand the biologic basis of their resistance. Recent studies on the biology of the cyst walls of Entamoeba histolytica and Entamoeba invadens have considerably advanced knowledge on the cellular processes involved in the transport and surface deposition of the main cyst wall components. Using transmission electron microscopy, cyto ... | 2011 | 21883633 |
| entamoeba histolytica: cloning, expression and evaluation of the efficacy of a recombinant amebiasis cysteine proteinase gene (acp1) antigen in minipig. | the amebiasis cysteine proteinase gene (acp1) encoding an antigen from entamoeba histolytica, as well as the recombinant acp1, obtained by cloning and expression of the acp1 gene in heterologous host escherichia coli bl-21 (de3), were used to evaluate their ability to induce immune protective responses in minipig against challenge infection in a minipig -e. histolytica model. there was a 64.52% reduction (p<0.001) in the group of recovery of challenged e. histolytica compared with that in the co ... | 2011 | 22154977 |
| direct detection of the acetate-forming activity of the enzyme acetate kinase. | acetate kinase, a member of the acetate and sugar kinase-hsp70-actin (askha) enzyme superfamily(1-5), is responsible for the reversible phosphorylation of acetate to acetyl phosphate utilizing atp as a substrate. acetate kinases are ubiquitous in the bacteria, found in one genus of archaea, and are also present in microbes of the eukarya(6). the most well characterized acetate kinase is that from the methane-producing archaeon methanosarcina thermophila(7-14). an acetate kinase which can only ut ... | 2011 | 22214984 |
| trends and prevalence of intestinal parasites at a tertiary care center in lebanon over a decade. | intestinal parasitic infections or infestation are amongst the most prevalent infections worldwide. this study aimed at revealing the changing trends over a decade duration of intestinal parasites identified at a major tertiary care center in lebanon between 1997-1998 and 2007-2008. the total number of specimens tested were 14,771 for 1997-1998 vs 7477 for 2007-2008. the positive findings for parasites were 2077 (14%) vs 1047 (14%), respectively. the majority of recovered parasites in both study ... | 2011 | 22259902 |
| the majority of cockroaches from the samutprakarn province of thailand are carriers of parasitic organisms. | we undertook a study of the mechanical transmission of parasitic organisms in cockroaches in the samutprakarn province of thailand. in this study, 920 cockroaches were obtained from 18 open-air shopping markets in 5 districts and 1 subdistrict of this province. all cockroaches were captured during their feeding time in their natural habitat. direct wet smear and modified acid-fast bacilli staining were used to identify the parasites from the external surface or cuticle of the cockroaches. the re ... | 2011 | 27857676 |
| new insights into host-pathogen interactions during entamoeba histolytica liver infection. | amoebiasis is the third worldwide disease due to a parasite. the causative agent of this disease, the unicellular eukaryote entamoeba histolytica, causes dysentery and liver abscesses associated with inflammation and human cell death. during liver invasion, before entering the parenchyma, e. histolytica trophozoites are in contact with liver sinusoidal endothelial cells (lsec). we present data characterizing human lsec responses to interaction with e. histolytica and identifying amoebic factors ... | 2011 | 24466432 |
| caecal amoebic colitis mimicking a colorectal cancer. | intestinal entamoeba histolytica infection in the uk is rare but it can lead to colitis, abscess formation, colonic perforation and rarely amoeboma. although most patients are asymptomatic, it can present with a wide range of gastrointestinal symptoms depending on the phase of the infection. we present a case of amoebic colitis that caused diagnostic uncertainty by mimicking a colonic carcinoma. | 2011 | 24972391 |
| amoebic ulcer of the male genitala: a rare case report. | amoebic ulcer of the penis is a very rare clinical entity. we report a case of amoebic ulcer of the glans penis in a 47-year-old male homosexual, symptomatic with severe pain and foul-smelling hemopurulent discharge of acute onset. he had received systemic antibiotics like ciprofloxacin and azithromycin prior to presentation with no improvement. diagnosis was confirmed by wet mount microscopic examination of the discharge. the patient responded well to a course of metronidazole. | 2010 | 21716788 |
| prevalence of intestinal parasitic infections among mentally disabled children and adults of urmia, iran. | the prevalence of intestinal parasites infection in institutions for mental retardation of urmia city, west azerbaijan province, iran was investigated. | 2010 | 22347245 |
| parasitic infection in a young man presenting with non-specific abdominal pain. | a 27-year-old man presented with a two-week history of central colicky abdominal pain associated with loose stools. further history revealed that he had been exposed to contaminated waters. stool investigation by direct wet stool smears revealed the presence of entamoeba histolytica and blastocystis hominis cysts. a diagnosis of amoebiasis secondary to e. histolytica and concurrent b. hominis infestation was made. we would like to emphasise the importance of clinical history including recent tra ... | 2010 | 25606208 |
| evaluation of a single pcr assays on cp5 gene for differentiation of entamoeba histolytica and e. dispar. | we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differentiate the pathogenic species, entamoeba histolytica, from the non-pathogenic species, e. dispar. | 2010 | 23113039 |
| the antiretroviral lectin cyanovirin-n targets well-known and novel targets on the surface of entamoeba histolytica trophozoites. | entamoeba histolytica, the protist that causes amebic dysentery and liver abscess, has a truncated asn-linked glycan (n-glycan) precursor composed of seven sugars (man(5)glcnac(2)). here, we show that glycoproteins with unmodified n-glycans are aggregated and capped on the surface of e. histolytica trophozoites by the antiretroviral lectin cyanovirin-n and then replenished from large intracellular pools. cyanovirin-n cocaps the gal/galnac adherence lectin, as well as glycoproteins containing o-p ... | 2010 | 20852023 |
| rotenoids from lablab purpureus l. and their bioefficacy against human disease vectors. | various plant parts of lablab purpureu s l. were collected and analyzed separately for their rotenoid content. among the plant parts, the maximum content was in the roots and minimum in the seeds. the identity of different rotenoids was confirmed by melting point, mixed melting point, uv, and infrared spectral studies and gas-liquid chromatography. six rotenoids (deguelin, dehydrodeguelin, rotenol, rotenone, tephrosin, and sumatrol) were isolated, identified, and quantified both in vivo and in v ... | 2010 | 20803155 |
| amebic liver abscess. | 2010 | 21036819 | |
| acute amebic appendicitis: report of a rare case. | acute appendicitis of amebic origin is considered a rare cause of acute appendicitis. we report a case of amebic appendicitis presenting with fever, severe pain in the right lower quadrant of the abdomen and rebound tenderness. lab investigations revealed neutrophilic leukocytosis. the patient underwent appendectomy. histopathological examination revealed numerous entameba histolytica trophozoites in the mucosa of the appendix. acute appendicitis of amebic origin does not appear frequently. appe ... | 2010 | 21045411 |
| [evolution of the prevalence the enteroparasitoses in talca-chile]. | the prevalence of intestinal parasites in preschool and school children in the city of talca and rural areas belonging to the maule region, was assessed annually by means of the serial parasitological studies in stool which were performed in the parasitology laboratory of the "universidad de talca". for consecutive periods since 1980 until 2008, an estimated prevalence of parasitism of 76.2% in the population studied was found. these results show a marked decrease from 9.8% to 2.5% in pathogenic ... | 2010 | 21046719 |
| evaluation of multiplex tandem real-time pcr for detection of cryptosporidium spp., dientamoeba fragilis, entamoeba histolytica, and giardia intestinalis in clinical stool samples. | the aim of this study was to describe the first development and evaluation of a multiplex tandem pcr (mt-pcr) assay for the detection and identification of 4 common pathogenic protozoan parasites, cryptosporidium spp., dientamoeba fragilis, entamoeba histolytica, and giardia intestinalis, from human clinical samples. a total of 472 fecal samples submitted to the department of microbiology at st. vincent's hospital were included in the study. the mt-pcr assay was compared to four real-time pcr (r ... | 2010 | 21048004 |
| in vitro trna methylation assay with the entamoeba histolytica dna and trna methyltransferase dnmt2 (ehmeth) enzyme. | protozoan parasites are among the most devastating infectious agents of humans responsible for a variety of diseases including amebiasis, which is one of the three most common causes of death from parasitic disease. the agent of amebiasis is the amoeba parasite entamoeba histolytica that exists under two stages: the infective cyst found in food or water and the invasive trophozoite living in the intestine. the clinical manifestations of amebiasis range from being asymptomatic to colitis, dysente ... | 2010 | 21048666 |
| synthesis, characterization, antiamoebic activity and cytotoxicity of new pyrazolo[3, 4-d]pyrimidine-6-one derivatives. | a new series of pyrazolo[3,4-d]pyrimidine-6-one derivatives (2a-2j) were prepared by using the biginelli multicomponent cyclocondensation of 3-methyl-1-phenyl-1h-pyrazol-5(4h)-one (1a), different aromatic aldehydes, and urea with a catalytic amount of hcl at reflux temperature. these compounds were characterized by ir, (1)h nmr, (13)c nmr, and mass spectral data. in vitro antiamoebic activity was performed against hm1:imss strain of entamoeba histolytica. the results showed that the compounds 2b ... | 2010 | 21054147 |
| [adaptation of a sensitive dna extraction method for detection of entamoeba histolytica by real-time polymerase chain reaction.] | this study was aimed to adapt a sensitive dna extraction protocol in stool samples for real-time polymerase chain reaction (pcr) detection of entamoeba histolytica which causes important morbidity and mortality worldwide. stool extraction is a problematic step and has direct effects on pcr sensitivity. in order to improve the sensitivity of e.histolytica detection by real-time pcr, "qiaamp dna stool minikit (qiagen, germany)" was modified by adding an overnight incubation step with proteinase k ... | 2010 | 21063995 |
| entamoeba histolytica infection and secreted proteins proteolytically damage enteric neurons. | the enteric protozoan parasite entamoeba histolytica causes amebic colitis through disruption of the mucus layer, followed by binding to and destruction of epithelial cells. however, it is not known whether ameba infections or ameba components can directly affect the enteric nervous system. analysis of mucosal innervations in the mouse model of cecal amebiasis showed that axon density was diminished to less than 25% of control. to determine whether amebas directly contributed to axon loss, we te ... | 2010 | 20855514 |
| infectious colitis. | purpose of review: the incidence of gastrointestinal infections continues to increase and infectious colitis contributes to significant morbidity and mortality worldwide. the purpose of this review is to highlight the recent advances in knowledge of pathogens causing infectious colitis. we describe the various pathogens and specifically focus on enterohemorrhagic escherichia coli (ehec) o157:h7, salmonella, shigella, campylobacter, and entamoeba histolytica infections, and their impact on long-t ... | 2010 | 20856114 |
| classification of protein kinases on the basis of both kinase and non-kinase regions. | protein phosphorylation is a generic way to regulate signal transduction pathways in all kingdoms of life. in many organisms, it is achieved by the large family of ser/thr/tyr protein kinases which are traditionally classified into groups and subfamilies on the basis of the amino acid sequence of their catalytic domains. many protein kinases are multi-domain in nature but the diversity of the accessory domains and their organization are usually not taken into account while classifying kinases in ... | 2010 | 20856812 |
| the domain structure of entamoeba α-actinin2. | entamoeba histolytica, a major agent of human amoebiasis, expresses two distinct forms of α-actinin, a ubiquitous actin-binding protein that is present in most eukaryotic organisms. in contrast to all metazoan α-actinins, in both isoforms the intervening rod domain that connects the n-terminal actin-binding domain with the c-terminal ef-hands is much shorter. it is suggested that these α-actinins may be involved in amoeboid motility and phagocytosis, so we cloned and characterised each domain of ... | 2010 | 20865366 |
| redox regulation of udp-glucose pyrophosphorylase from entamoeba histolytica. | amoebiasis is an intestinal infection caused by the human pathogen entamoeba histolytica and representing the third leading cause of death by parasites in the world. host-parasite interactions mainly involve anchored glycoconjugates localized in the surface of the parasitic cell. in protozoa, synthesis of structural oligo- and polysaccharides occurs via udp-glucose, generated in a reaction catalyzed by udp-glucose pyrophosphorylase. we report the molecular cloning of the gene coding for this enz ... | 2010 | 20888387 |
| development of multiplex real-time polymerase chain reaction for detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in clinical specimens. | multiplex real-time polymerase chain reaction (pcr) was developed for differential detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii. specific primers were designed for all three species, and then differentiation of e. histolytica and e. dispar was achieved simultaneously using a hybridization probe and melting curve analysis, whereas e. moshkovskii was detected with a separate probe under the same condition. this assay detected as little as 0.2 pg of e. histolytica ... | 2010 | 20889890 |
| seroprevalence of entamoeba histolytica infection among men who have sex with men in sydney, australia. | a retrospective analysis was undertaken to determine the seroprevalence of entamoeba histolytica infection in sydney, australia. using an enzyme-linked immunosorbent assay, 429 high risk human immunodeficiency virus (hiv)-infected men who have sex with men (msm), 446 low risk hiv-uninfected msm, and 456 hiv-uninfected controls were assessed. seroprevalence rates were 5.13% for the high risk hiv-infected msm group, 0.22% for the low risk hiv-uninfected msm group, and 0.44% for the control group. ... | 2010 | 20889891 |
| entamoeba histolytica calreticulin: an endoplasmic reticulum protein expressed by trophozoites into experimentally induced amoebic liver abscesses. | entamoeba histolytica calreticulin (ehcrt) is remarkably immunogenic in humans (90-100% of invasive amoebiasis patients). nevertheless, the study of calreticulin in this protozoan is still in its early stages. the exact location, biological functions, and its role in pathogenesis are yet to be fully understood. the aim of the present work is to determine the location of ehcrt in virulent trophozoites in vivo and the expression of the ehcrt gene during the development of experimentally induced am ... | 2010 | 20922421 |
| comparison of protein-free defined media, and effect of l-cysteine and ascorbic acid supplementation on viability of axenic entamoeba histolytica. | entamoeba histolytica is the etiologic agent for amoebiasis. the excretory-secretory (es) products of the trophozoites contain virulence factors and antigens useful for diagnostic applications. contaminants from serum supplements and dead trophozoites impede analysis of es. therefore, a protein-free medium that can sustain maximum viability of e. histolytica trophozoites for the longest time duration will enable collection of contaminant-free and higher yield of es products. in the present study ... | 2010 | 20922423 |
| metabolome analysis revealed increase in s-methylcysteine and phosphatidylisopropanolamine synthesis upon l-cysteine deprivation in the anaerobic protozoan parasite entamoeba histolytica. | l-cysteine is ubiquitous in all living organisms and is involved in a variety of functions, including the synthesis of iron-sulfur clusters and glutathione and the regulation of the structure, stability, and catalysis of proteins. in the protozoan parasite entamoeba histolytica, the causative agent of amebiasis, l-cysteine plays an essential role in proliferation, adherence, and defense against oxidative stress; however, the essentiality of this amino acid in the pathways it regulates is not wel ... | 2010 | 20923776 |
| trend of entamoeba histolytica infestation in kolkata. | abstract: | 2010 | 20925925 |
| [amebiasis: implications of the recognition of entamoeba dispar and the identification of entamoeba moshkovskii in humans]. | the history of entamoeba histolytica is very confuse and shows several wrong concepts about the parasite and its relationship with the host. the poor correlation between the prevalence of asymptomatic and symptomatic amebiasis originated the proposal of three explicative hypothesis, among them was the concept of brumpt that e. histolytica comprised two morphologically identical species, e. dysenteriae and e. dispar. the application of modern molecular techniques irrefutably proved that e. histol ... | 2010 | 20928980 |
| anti-inflammatory defense mechanisms of entamoeba histolytica. | the monocyte locomotion inhibitory factor (mlif), a heat-stable oligopeptide found in the supernatant fluid of entamoeba histolytica axenic cultures, may contribute to the delayed inflammation observed in amoebic hepatic abscess. this factor was isolated by ultra-filtration and high powered liquid chromatography, obtaining a primary met-gln-cys-asn-ser structure, identified afterwards as the carboxyl-terminal (…cys-asn-ser) active site. the selective anti-inflammatory effects of the pentapeptide ... | 2010 | 20938711 |
| [entamoeba histolytica]. | 2010 | 20942060 | |
| ehmapk, the mitogen-activated protein kinase from entamoeba histolytica is associated with cell survival. | mitogen activated protein kinases (mapks) are a class of serine/threonine kinases that regulate a number of different cellular activities including cell proliferation, differentiation, survival and even death. the pathogen entamoeba histolytica possess a single homologue of a typical mapk gene (ehmapk) whose identification was previously reported by us but its functional implications remained unexplored. ehmapk, the only mitogen-activated protein kinase from the parasitic protist entamoeba histo ... | 2010 | 20949043 |
| crystal structure of the cysteine protease inhibitor 2 from entamoeba histolytica: functional convergence of a common protein fold. | cysteine proteases (cp) are key pathogenesis and virulence determinants of protozoan parasites. entamoeba histolytica contains at least 50 cysteine proteases; however, only three (ehcp1, ehcp2 and ehcp5) are responsible for approximately 90% of the cysteine protease activity in this parasite. cps are expressed as inactive zymogens. because the processed proteases are potentially cytotoxic, protozoan parasites have developed mechanisms to regulate their activity. inhibitors of cysteine proteases ... | 2010 | 20951777 |
| [distribution of intestinal parasites detected in the tokat public health laboratory during the period from january 2007 - december 2009]. | in this study, the results of 1868 stool samples and 215 cellophane tapes examined in the tokat public health laboratory between january 2007-december 2009 and the distribution of these parasites were evaluated retrospectively. a total of 1868 stool specimens from 1146 (61.3%) males and 722 (38.7%) females were examined for intestinal parasites using direct examination and the formol-ethyl acetate concentration methods. intestinal parasites were found in 40 (2.1%) females and 85 (4.6%) males. a ... | 2010 | 20954116 |
| [a retrospective evaluation of the prevalence of intestinal parasites in istanbul, turkey]. | the aim of this study is to estimate the prevalence of intestinal parasites, in comparison to previous studies in istanbul and different geographic regions of turkey. between january 1999-december 2009, a total of 27664 stool samples from patients with suspicious intestinal parasitic infections who were admitted to istanbul university cerrahpasa medical school hospital were examined for intestinal parasites by using native lugol and formalin-ethyl acetate concentration technique, in adition to t ... | 2010 | 20954117 |
| synthesis, characterization, antiamoebic activity and cytotoxicity of novel 2-(quinolin-8-yloxy) acetohydrazones and their cyclized products (1,2,3-thiadiazole and 1,2,3-selenadiazole derivatives). | a series of 1,2,3-thiadiazole and 1,2,3-selenadiazole derivatives were synthesized by the cyclization of novel 2-(quinolin-8-yloxy) acetohydrazones. in vitro antiamoebic activity was performed against hm1: imss strain of entamoeba histolytica. the results showed that all the 2-(quinolin-8-yloxy) acetohydrazones were more active than their cyclized products (1,2,3-thiadiazole and 1,2,3-selenadiazole derivatives). sar showed that the compounds having quinoline ring and hydrazone linkage with free ... | 2010 | 20961670 |
| hepato-pulmonary amebiasis: a case report. | infections with entamoeba histolytica are seen worldwide and are more prevalent in the tropics. about 90% of infections are asymptomatic, and the remaining 10% produce a spectrum of clinical syndromes, ranging from dysentery to abscesses of the liver or other organs. extra-intestinal infection by e. histolytica most often involves liver. pleuro-pulmonary involvement, seen as the second most common extra-intestinal pattern of infection, is frequently associated with amebic liver abscess. pulmonar ... | 2010 | 20963322 |
| dynamic instability of the intracellular pressure drives bleb-based motility. | we have demonstrated that the two- and three-dimensional motility of the human pathogenic parasite entamoeba histolytica (eh) depends on sustained instability of the intracellular hydrostatic pressure. this instability drives the cyclic generation and healing of membrane blebs, with typical protrusion velocities of 10-20 μm/second over a few hundred milliseconds and healing times of 10 seconds. the use of a novel micro-electroporation method to control the intracellular pressure enabled us to de ... | 2010 | 20980385 |
| case report: huge amoebic liver abscesses in both lobes. | we describe the case of a patient who returned to china from africa and underwent emergency open surgical drainage with evacuation of 600 ml of anchovy sauce-like fluid from hepatic lesions. computed tomography scans and surgical findings indicated abscesses in both hemilivers and communication between them. bacteriological investigation of the fluid yielded negative results, but dna assay of the pus detected 18s rrna genes of entamoeba histolytica. serum anti-amoebic antibodies were detected us ... | 2010 | 20811141 |
| approaches to characterizing entamoeba histolytica transcriptional regulation. | entamoeba histolytica causes an estimated 100,000 deaths per year and is one of the leading causes of death among parasitic infections. studies using e. histolytica-specific polymerase chain reaction identified that 13.8% of adults in a rural mexican community and 11.2% of adults in central vietnam are asymptomatically colonized. such high incidents of asymptomatic infection suggest that only a minority of infections proceed to invasive disease. understanding the mechanisms that underpin variabl ... | 2010 | 20812994 |
| polymerase chain reaction confirmation of diagnosis of intestinal amebiasis in puducherry. | stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic entamoeba dispar and entamoeba moshkovskii. | 2010 | 20814775 |
| mass infection with entamoeba histolytica in a japanese institution for individuals with mental retardation: epidemiology and control measures. | after two cases of amoebic colitis were detected at an institution for the mentally retarded in the yamagata prefecture of japan, the prevalence and epidemiology of entamoeba histolytica infection at the institution were investigated. when the 76 residents with mental retardation were checked by serology and stool examinations, 40 (53%) showed evidence of infection with e. histolytica (i.e. e. histolytica-specific antibodies in their serum, entamoeba cysts in their stools, and/or e. histolytica- ... | 2010 | 20819306 |
| intestinal parasites infection among immunocompromised patients in riyadh, saudi arabia. | this study was conducted to determine the prevalence of intestinal parasites among immunocompromised patients in riyadh, saudi arabia. fecal samples were collected from 136 immunocompromised patients (52 females and 84 males), aged 2 to 69 years. specimens were fixed with 10% formalin for 30 min (2-3 g fecal(-1)) and then concentrated by a formalin-ether sedimentation technique. samples were examined as wet saline mounts and in iodine preparation for detection of protozoan oocysts, cysts, helmin ... | 2010 | 20836300 |
| entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates nfkappab-mediated pro-inflammatory responses. | integrins are important mammalian receptors involved in normal cellular functions and the pathogenesis of inflammation and disease. entamoeba histolytica is a protozoan parasite that colonizes the gut, and in 10% of infected individuals, causes amebic colitis and liver abscess resulting in 10(5) deaths/year. e. histolytica-induced host inflammatory responses are critical in the pathogenesis of the disease, yet the host and parasite factors involved in disease are poorly defined. here we show tha ... | 2010 | 20837477 |
| chemical constituents of osyris alba and their antiparasitic activities. | phytochemical investigation of osyris alba l. (santalaceae) of jordanian origin resulted in the isolation and identification of one new pyrrolizidine alkaloid, osyrisine (1), together with 16 other known compounds. the structures of all compounds were established on the basis of spectroscopic analysis. osyrisine, catechin, and catechin-3-o-α-l-rhamnopyranoside exhibited a significant level of antiparasitic activity against two parasites, entamoeba histolytica and giardia intestinalis. | 2010 | 20839131 |
| medicinal potions used against infectious bowel diseases in mayan traditional medicine. | since one of the main health problems of the indigenous population are infectious bowel diseases, we decided to test mayan medicinal potions used to treat these conditions against some of the causal agents. | 2010 | 20728517 |
| in vivo visualization of trophozoites in patients with amoebic colitis by using a newly developed endocytoscope. | the endocytoscopy system (ecs) is a new method to provide real-time super-magnifying microscopic imaging in vivo. routine diagnosis of amebic colitis requires multiple tests that are both time consuming and costly. | 2010 | 20579647 |
| downregulation of an entamoeba histolytica rhomboid protease reveals roles in regulating parasite adhesion and phagocytosis. | entamoeba histolytica is a deep-branching eukaryotic pathogen. rhomboid proteases are intramembrane serine proteases, which cleave transmembrane proteins in, or in close proximity to, their transmembrane domain. we have previously shown that e. histolytica contains a single functional rhomboid protease (ehrom1) and has unique substrate specificity. ehrom1 is present on the trophozoite surface and relocalizes to internal vesicles during erythrophagocytosis and to the base of the cap during surfac ... | 2010 | 20581296 |
| entamoeba histolytica liver abscess. | 2010 | 20584930 | |
| an unusual presentation of amebic liver abscesses. | 2010 | 20584933 | |
| infection status of hospitalized diarrheal patients with gastrointestinal protozoa, bacteria, and viruses in the republic of korea. | to understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixed-infection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the republic of korea. a total of 76,652 stool samples were collected from 96 hospitals across the nation. the positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. especially, cryptosporidium parvum was highly mixed ... | 2010 | 20585526 |
| a case of probable mixed-infection with clonorchis sinensis and fasciola sp.: ct and parasitological findings. | we report here a human case probably mixed-infected with clonorchis sinensis and fasciola sp. who was diagnosed by computed tomography (ct) scan, serological findings, and/or fecal examination. the patient was a 43-year-old korean female and was admitted to kyung hee university hospital with the complaints of fever and abdominal pain. on admission, marked eosinophilia was noted in her peripheral blood. ct scan showed specific lesions for clonorchiasis and fascioliasis in the liver, along with le ... | 2010 | 20585533 |
| two atypical l-cysteine-regulated nadph-dependent oxidoreductases involved in redox maintenance, l-cystine and iron reduction, and metronidazole activation in the enteric protozoan entamoeba histolytica. | we discovered novel catalytic activities of two atypical nadph-dependent oxidoreductases (ehno1/2) from the enteric protozoan parasite entamoeba histolytica. ehno1/2 were previously annotated as the small subunit of glutamate synthase (glutamine:2-oxoglutarate amidotransferase) based on similarity to authentic bacterial homologs. as e. histolytica lacks the large subunit of glutamate synthase, ehno1/2 were presumed to play an unknown role other than glutamine/glutamate conversion. transcriptomic ... | 2010 | 20592025 |
| identification and delineation of members of the entamoeba complex by pyrosequencing. | a method using a single-round pcr coupled to pyrosequencing was developed for the detection and differentiation of members of the entamoeba complex. the technique was evaluated using dna isolated directly from faecal specimens and compared with a duplex real-time pcr targeting entamoeba histolytica and entamoeba dispar, and a conventional single-round pcr for the detection of entamoeba moshkovskii. tetranucleate cysts from 102 faecal specimens from swedish, danish and dutch patients test-positiv ... | 2010 | 20691255 |
| synthesis, characterization, antiamoebic activity and cytotoxicity of novel series of pyrazoline derivatives bearing quinoline tail. | the cyclization of chalcones (1a-1j) with 2-(quinolin-8-yloxy) acetohydrazide (2) under basic condition led to the formation of new compounds, pyrazoline derivatives (3a-3j). in vitro antiamoebic activity was performed against hm1: imss strain of entamoeba histolytica. the results showed that the compounds 3d, 3g, 3h, and 3j exhibited promising antiamoebic activity (ic(50) = 0.05 microm, 0.31 microm, 0.06 microm, 0.29 microm) respectively than the standard drug metronidazole (ic(50) = 1.84 micro ... | 2010 | 20696501 |
| geographical location and age affects the incidence of parasitic infestations in school children. | environmental factors affect the dissemination and distribution of intestinal parasites in human communities. to comprehend the prevalence of parasitic infestation and to examine whether geographical location and age also influence the prevalence of infection, fecal samples from 195 school children (rural = 95; male = 39; female = 56) (urban = 100; male = 60; female = 40) of five age groups ranging from 5 to 11 years in two different socio-economic zones (rural and urban) were screened for speci ... | 2010 | 20699511 |
| a nuclear family a dna polymerase from entamoeba histolytica bypasses thymine glycol. | eukaryotic family a dna polymerases are involved in mitochondrial dna replication or translesion dna synthesis. here, we present evidence that the sole family a dna polymerase from the parasite protozoan e. histolytica (ehdnapola) localizes to the nucleus and that its biochemical properties indicate that this dna polymerase may be involved in translesion dna synthesis. | 2010 | 20706627 |
| evaluation of the prevalence of enterotoxigenic bacteroides fragilis and the distribution bft gene subtypes in patients with diarrhea. | the aim of this study was to determine the prevalence of enterotoxigenic bacteroides fragilis (etbf) in the patients with diarrhea in our region and to assess the association between diarrhea and bft gene subtypes. the presence of etbf and bft gene subtypes were investigated in 200 stool samples from patients with diarrhea, diagnosed as gastroenteritis, which were sent to clinical microbiology laboratory at zonguldak karaelmas university, training and research hospital and in 200 stool samples f ... | 2010 | 20709181 |
| a novel galacto-glycerolipid from oxalis corniculata kills entamoeba histolytica and giardia lamblia. | oxalis corniculata is a naturally occurring weed that has been used in traditional medicine for the cure of dysentery and diarrhea in india. one of the common causes of dysentery is due to infection by the protist pathogen entamoeba histolytica. bioactivity profiling of extracts from o. corniculata identified several compounds that showed antiamoebic activity in axenic cultures of e. histolytica. these were characterized by nuclear magnetic resonance, infrared, and mass spectrometry as (i) oc-1, ... | 2010 | 20713666 |
| dynamical organization of the cytoskeletal cortex probed by micropipette aspiration. | bleb-based cell motility proceeds by the successive inflation and retraction of large spherical membrane protrusions ("blebs") coupled with substrate adhesion. in addition to their role in motility, cellular blebs constitute a remarkable illustration of the dynamical interactions between the cytoskeletal cortex and the plasma membrane. here we study the bleb-based motions of entamoeba histolytica in the constrained geometry of a micropipette. we construct a generic theoretical model that combine ... | 2010 | 20713731 |
| [two cases of pulmonary amebiasis]. | case 1: a 74-year-old man having a week's fever and diagnosed with a liver abscess was treated with several antibiotics and percutaneous liver drainage. his respiration gradually worsened and chest computed tomography (ct) showed right pleural effusion and a left-lung mass. percutaneous fine needle aspiration of the pulmonary mass detected entamoeba histolytica. case 2: a 44-year old, zoo office worker admitted for fever and right chest pain was found in ct to have right pleural effusion and a m ... | 2010 | 20715559 |
| structure-activity relationships of mononuclear metal-thiosemicarbazone complexes endowed with potent antiplasmodial and antiamoebic activities. | a useful concept for the rational design of antiparasitic drug candidates is the complexation of bioactive ligands with transition metals. in view of this, an investigation was conducted into a new set of metal complexes as potential antiplasmodium and antiamoebic agents, in order to examine the importance of metallic atoms, as well as the kind of sphere of co-ordination, in these biological properties. four functionalized furyl-thiosemicarbazones (nt1-4) treated with divalent metals (cu, co, pt ... | 2010 | 20719524 |
| difficulties in the treatment of intestinal amoebiasis in mentally disabled individuals at a rehabilitation institution for the intellectually impaired in japan. | a mass entamoeba histolytica infection recurred despite repeated treatments with metronidazole at a rehabilitation institution for individuals with intellectual impairments. | 2010 | 20720419 |
| biochemical characterization of the dna ligase i from entamoeba histolytica. | dna ligases play an essential role in dna replication and repair. herein, we report the cloning and biochemical characterization of dna ligase i from the protozoan parasite entamoeba histolytica (ehdnaligi). ehdnaligi is an atp-dependent dna ligase of 685 amino acids with 35% identity to human dna ligase i. this report shows that heterologous expressed ehdnaligi is able to perform the three conserved steps of a dna ligation reaction: adenylation, binding to a 5'-phosphorylated nicked dna substra ... | 2010 | 20603158 |
| synthesis and antimicrobial and nitric oxide synthase inhibitory activities of novel isothiourea derivatives. | the reaction of substituted benzylhalides, or of halomethyl derivatives of thiophene or furane, with thiourea or its derivatives yielded the respective isothioureas as hydrohalide salts. the products (a total of 17, including 16 novel compounds) were tested for activity against five gram-positive and nine gram-negative bacterial strains, six yeast species and two protozoan species. the most active against gram-positive bacteria were s-(2,4-dinitrobenzyl)isothiourea hydrochloride (mic range for f ... | 2010 | 20607486 |