Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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sucrose transporters of higher plants. | recent advances have provided new insights into how sucrose is moved from sites of synthesis to sites of utilisation or storage in sink organs. sucrose transporters play a central role, as they orchestrate sucrose allocation both intracellularly and at the whole plant level. sucrose produced in mesophyll cells of leaves may be effluxed into the apoplasm of mesophyll or phloem parenchyma cells by a mechanism that remains elusive, but experimentally consistent with facilitated transport or energy- ... | 2010 | 20303321 |
selenium accumulation by forage and grain crops and volatilization from seleniferous soils amended with different organic materials. | greenhouse and field experiments were conducted to study the direct and residual effect of applying different organic materials on se accumulation by crops and volatilization from seleniferous soils of northwestern india. among organic manures, press mud and poultry manures proved 4-5 times more effective in reducing se accumulation by different crops than farmyard manure. efficiency of organic manures increased with increase in application rates. application of both press mud and poultry manure ... | 2010 | 20015536 |
pretreatment of siam weed stem by several chemical methods for increasing the enzymatic digestibility. | siam weed [chromolaena odorata (l.) king & robinson], an invasive exotic weed in china, was proposed as a feedstock for bioethanol production. this would be a promising way of using for an invasive weed that needs management and control. it was found that the glucan content of the weed stem was similar to that of sugarcane bagasse, but higher than those of corn stover and wheat straw. several chemical pretreatment methods were applied to the weed stem to increase its enzymatic digestibility. mil ... | 2010 | 20349449 |
mitochondria in the midgut epithelial cells of sugarcane borer parasitized by cotesia flavipes (cameron, 1891). | the sugarcane borer diatraea saccharalis (lepidoptera: crambidae) has been controlled by cotesia flavipes (hymenoptera: braconidae); however, very little is known about the effect of the parasitism in the host organs, including the midgut. this work aims to verify mitochondrial alteration in the different midgut epithelial cells of d. saccharalis parasitized by c. flavipes. midgut fragments (anterior and posterior region) of both non-parasitized and parasitized larvae were processed for transmis ... | 2010 | 20231974 |
population genetics of eldana saccharina walker (lepidoptera: pyralidae) and the implications for management using biocontrol. | the african sugarcane stalk borer, eldona saccharina walker (lepidoptera: pyralidae), is widely distributed throughout sub-saharan africa and is an important insect pest of maize and sugarcane. the insect shows significant variation in behaviour, host plant and natural enemy guild in different regions. several attempts to redistribute the natural enemies of e. saccharina from west africa to south africa were unsuccessful. the significant behavioural, host plant and natural enemy variations as we ... | 2010 | 21539263 |
within-field damage and distribution patterns of the stalk borer, eldana saccharina (lepidoptera: pyralidae), in sugarcane and a comparison with nematode damage. | the occurrence of eldana saccharina (lepidoptera: pyralidae) was monitored in grids represented by plots in 12 nematicide trials in south african sugarcane fields. the trials encompassed a total of eight plant cane crops and 22 ratoon crops and were situated within commercial cane fields. several measurements were made to characterize the damage caused by e. saccharina. these included the number of internodes per stalk, the percentage of internodes damaged and the percentage of stalks damaged. t ... | 2010 | 19860988 |
life table studies of elasmopalpus lignosellus (lepidoptera: pyralidae) on sugarcane. | the lesser cornstalk borer, elasmopalpus lignosellus (zeller) (lepidoptera: pyralidae) is an important pest of sugarcane (a complex hybrid of saccharum spp.) in southern florida. reproductive and life table parameters for e. lignosellus were examined at nine constant temperatures from 13 to 36 °c with sugarcane as the larval food source. the pre- and postoviposition periods decreased with increasing temperatures and reached their minimums at 33 and 36 °c, respectively. the oviposition period was ... | 2010 | 22182570 |
geographic variation of sex pheromone and mitochondrial dna in diatraea saccharalis (fab., 1794) (lepidoptera: crambidae). | (9z,11e)-hexadecadienal and (z11)-hexadecenal, the main sex pheromone components of the sugarcane borer, diatraea saccharalis, were identified and quantified from four brazilian and one colombian populations using gc-ead, gc-ms and gc analyses. three different ratios were observed, 9:1, 6:1, and 3:1. the pheromone concentration for the major component, (9z,11e)-hexadecadienal, varied from 6.8 ng/gland to 21.9 ng/gland and from 1.7 ng/gland to 6.5 to the minor component, (z11)-hexadecenal. the 25 ... | 2010 | 20558173 |
rice tillering and yield as affected by artificial and sugarcane borer (lepidoptera: crambidae) culm injury. | a 2-yr study was conducted to evaluate the tillering and yield response of rice, oryza sativa l., whose culms were injured artificially or by larval sugarcane borers, diatraea saccharalis (f.). artificially injured plants produced approximately 0.49 more tillers than uninjured plants, similar to what has previously been reported for larval injured plants. in contrast, artificial injury did not affect yield per tiller, whereas larval injury did. the proximity of larval injury to the panicle had a ... | 2010 | 20388284 |
valorization of an industrial organosolv-sugarcane bagasse lignin: characterization and use as a matrix in biobased composites reinforced with sisal fibers. | in the present study, the main focus was the characterization and application of the by-product lignin isolated through an industrial organosolv acid hydrolysis process from sugarcane bagasse, aiming at the production of bioethanol. the sugarcane lignin was characterized and used to prepare phenolic-type resins. the analysis confirmed that the industrial sugarcane lignin is of hgs type, with a high proportion of the less substituted aromatic ring p-hydroxyphenyl units, which favors further react ... | 2010 | 20589841 |
genetic variability of mahanarva sp (hemiptera: cercopidae) collected from different sites in brazil. | spittlebugs are the leading cause of damage to tall grasses. annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. correct identification of these species is difficult due to similarities in color, body size and male genitalia. molecular markers have been useful in the identification and assessment of genetic diversity of many species. we investigated the genetic diversity of the spittlebug species mahanarva fimbriolata, m. spectabilis ... | 2010 | 20533195 |
purification and biochemical properties of a glucose-stimulated beta-d-glucosidase produced by humicola grisea var. thermoidea grown on sugarcane bagasse. | the effect of several carbon sources on the production of mycelial-bound beta-glucosidase by humicola grisea var. thermoidea in submerged fermentation was investigated. maximum production occurred when cellulose was present in the culture medium, but higher specific activities were achieved with cellobiose or sugarcane bagasse. xylose or glucose (1%) in the reaction medium stimulated beta-glucosidase activity by about 2-fold in crude extracts from mycelia grown in sugarcane bagasse. the enzyme w ... | 2010 | 20221730 |
use of brazilian sugarcane bagasse ash in concrete as sand replacement. | sugarcane today plays a major role in the worldwide economy, and brazil is the leading producer of sugar and alcohol, which are important international commodities. the production process generates bagasse as a waste, which is used as fuel to stoke boilers that produce steam for electricity cogeneration. the final product of this burning is residual sugarcane bagasse ash (sba), which is normally used as fertilizer in sugarcane plantations. ash stands out among agroindustrial wastes because it re ... | 2010 | 20163947 |
xylooligosaccharides production from alkali-pretreated sugarcane bagasse using xylanases from thermoascus aurantiacus. | sugarcane bagasse hemicellulose was isolated in a one-step chemical extraction using hydrogen peroxide in alkaline media. the polysaccharide containing 80.9% xylose and small amounts of l-arabinose, 4-o-methyl-d-glucuronic acid and glucose, was hydrolyzed by crude enzymatic extracts from thermoascus aurantiacus at 50 degrees c. conditions of enzymatic hydrolysis leading to the best yields of xylose and xylooligosaccharides (dp 2-5) were investigated using substrate concentration in the range 0.5 ... | 2010 | 20066571 |
removal of zn2+ from aqueous single metal solutions and electroplating wastewater with wood sawdust and sugarcane bagasse modified with edta dianhydride (edtad). | this work describes the preparation of a new chelating material derived from wood sawdust, manilkara sp., and not only the use of a new support, but also a chemically modified sugarcane bagasse synthesized in our previous work to remove zn(2+) from aqueous solutions and electroplating wastewater. the first part describes the chemical modification of wood sawdust and sugarcane bagasse using ethylenediaminetetraacetic dianhydride (edtad) as modifying agent in order to introduce carboxylic acid and ... | 2010 | 20047793 |
efficient targeting of polyhydroxybutyrate biosynthetic enzymes to plant peroxisomes requires more than three amino acids in the carboxyl-terminal signal. | metabolic engineering of plant peroxisomes for biotechnological purposes typically requires efficient peroxisomal targeting of heterologous proteins. type i peroxisomal targeting signals (pts1) consist of three uncleaved amino acids (skl or a conserved variant) at the carboxyl terminus and direct nuclear-encoded proteins into the peroxisomes of eukaryotic cells. pts1 fusion with a heterologous protein results in peroxisomal targeting of that protein, but the minimal length of pts1 required for e ... | 2010 | 19879015 |
scale-up of diluted sulfuric acid hydrolysis for producing sugarcane bagasse hemicellulosic hydrolysate (sbhh). | sugarcane bagasse was pretreated with diluted sulfuric acid to obtain sugarcane bagasse hemicellulosic hydrolysate (sbhh). experiments were conducted in laboratory and semi-pilot reactors to optimize the xylose recovery and to reduce the generation of sugar degradation products, as furfural and 5-hydroxymethylfurfural (hmf). the hydrolysis scale-up procedure was based on the h-factor, that combines temperature and residence time and employs the arrhenius equation to model the sulfuric acid conce ... | 2010 | 19846294 |
identification and expression analysis of micrornas and targets in the biofuel crop sugarcane. | micrornas (mirnas) are small regulatory rnas, some of which are conserved in diverse plant genomes. therefore, computational identification and further experimental validation of mirnas from non-model organisms is both feasible and instrumental for addressing mirna-based gene regulation and evolution. sugarcane (saccharum spp.) is an important biofuel crop with publicly available expressed sequence tag and genomic survey sequence databases, but little is known about mirnas and their targets in t ... | 2010 | 21092324 |
phylogenetic relationships among saccharum clones in pakistan revealed by rapd markers. | forty sugarcane genotypes (clones), including elite lines, commercial cultivars of saccharum officinarum and s. barberi clones, were fingerprinted with 30 rapd markers, using a pcr-based marker assay. the genetic distance for rapd data was determined according to nei, and relationships between accessions were graphed in a dendrogram. genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. the lowest genetic distance was found between genotypes us-406 a ... | 2010 | 20799164 |
setaria viridis: a model for c4 photosynthesis. | c(4) photosynthesis drives productivity in several major food crops and bioenergy grasses, including maize (zea mays), sugarcane (saccharum officinarum), sorghum (sorghum bicolor), miscanthus x giganteus, and switchgrass (panicum virgatum). gains in productivity associated with c(4) photosynthesis include improved water and nitrogen use efficiencies. thus, engineering c(4) traits into c(3) crops is an attractive target for crop improvement. however, the lack of a small, rapid cycling genetic mod ... | 2010 | 20693355 |
gish characterization of erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids. | within erianthus, a genus close to saccharum, the species e. arundinaceus has the potential to contribute valuable traits to sugarcane, including adaptation to biotic and abiotic stresses and ratooning ability. sugarcane breeders have tried for a long time to use erianthus species in their breeding programs but until recently were constrained by a lack of fertile saccharum x erianthus hybrids. we report here for the first time the chromosome composition of fertile saccharum officinarum x e. arun ... | 2010 | 20616864 |
genetic studies of "noble cane" for identification and exploitation of genetic markers. | forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of saccharum officinarum and clones of s. barberi were fingerprinted with 50 ssr markers using a pcr-based marker assay. nei's genetic distances for ssr data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. the shortest genetic distance of 0.60 was seen betw ... | 2010 | 20533196 |
dna profiling of sugarcane genotypes using randomly amplified polymorphic dna. | dna profiles of 40 sugarcane genotypes were constructed with 30 rapd markers. sugarcane genotypes of both saccharum officinarum and s. barberi were included in this study. multiple alleles were detected from each rapd; there was a high level of polymorphism. on average, 7.93 alleles were produced per primer, giving a total of 238 alleles. the genetic distances between these genotypes were assessed with the popgene dna sequence analysis software. a dendrogram was constructed from these data; cult ... | 2010 | 20391332 |
adsorption behavior of heavy metals onto chemically modified sugarcane bagasse. | a new process for the xanthation of sugarcane (saccharum officinarum) bagasse was investigated for the separation of cadmium, lead, nickel, zinc and copper from their aqueous solutions. adsorption capacity of the charred xanthated sugarcane bagasse (cxsb) was found to be significantly more than the several biosorbents reported in the literatures. the modified material was characterized by ftir and elemental analysis. the kinetics of sorption of the tested metals was fast, reaching equilibrium wi ... | 2010 | 20006923 |
genetic diversity of saccharum officinarum accessions in pakistan as revealed by random amplified polymorphic dna. | genetic diversity of 20 sugarcane accessions in pakistan was studied using 21 random amplified polymorphic dna markers. the mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. this probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage of these varieties. among the varieties, none was found to be totally distinct and divergent from the others ... | 2009 | 19937582 |
impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane. | a cephalosporin antibiotic, cefotaxime (omnatax™) promoted somatic embryogenesis and subsequent shoot regeneration in vitro from spindle in sugarcane irrespective of the genotypes as (coj 83, coj 88 and coj 64) culturered on ms medium with 2,4-d (2.5 mgl(-1)) and kinetin (0.5 mgl(-1)). seven different concentrations of cefotaxime (100, 200, 300, 400, 500, 600 and 700 mgl(-1)) were tested to find the optimal concentration of cefotaxime for somatic embryogenesis from callus cultures. among the thr ... | 2009 | 23572935 |
growth at elevated co(2) delays the adverse effects of drought stress on leaf photosynthesis of the c(4) sugarcane. | sugarcane (saccharum officinarum l. cv. cp72-2086) was grown in sunlit greenhouses at daytime [co(2)] of 360 (ambient) and 720 (elevated)mumolmol(-1). drought stress was imposed for 13d when plants were 4 months old, and various photosynthetic parameters and levels of nonstructural carbohydrates were determined for uppermost fully expanded leaves of well-watered (control) and drought stress plants. control plants at elevated [co(2)] were 34% and 25% lower in leaf stomatal conductance (g(s)) and ... | 2009 | 18462832 |
enhancing plant uptake of polychlorinated biphenyls and cadmium using tea saponin. | the potential of natural surfactant tea saponin to enhance uptake of polychlorinated biphenyls (pcbs) and cadmium (cd) by zea mays l. and saccharum officinarum l. was investigated. with addition of tea saponin at 0.01% in solution culture, the concentrations of pcb 14, pcb 18, pcb 77 and pcb 156 in root of corn seedling were 2.72, 2.68, 1.94 and 2.40 times as those of treatments without adding any surfactant, respectively. application of tea saponin to the soil significantly elevated pcb 5 accum ... | 2009 | 19477119 |
targeted single nucleotide polymorphism (snp) discovery in a highly polyploid plant species using 454 sequencing. | discovering single nucleotide polymorphisms (snps) in specific genes in a heterozygous polyploid plant species, such as sugarcane, is challenging because of the presence of a large number of homologues. to discover snps for mapping genes of interest, 454 sequencing of 307 polymerase chain reaction (pcr) amplicons (> 59 kb of sequence) was undertaken. one region of a four-gasket sequencing run, on a 454 genome sequencer flx, was used for pooled pcr products amplified from each parent of a quantit ... | 2009 | 19386042 |
stem juice production of the c4 sugarcane (saccharum officinarum) is enhanced by growth at double-ambient co2 and high temperature. | two cultivars of sugarcane (saccharum officinarum cv. cp73-1547 and cp88-1508) were grown for 3 months in paired-companion, temperature-gradient, sunlit greenhouses under daytime [co2] of 360 (ambient) and 720 (double ambient) micromol mol(-1) and at temperatures of 1.5 degrees c (near ambient) and 6.0 degrees c higher than outside ambient temperature. leaf area and biomass, stem biomass and juice and co2 exchange rate (cer) and activities of ribulose bisphosphate carboxylase-oxygenase (rubisco) ... | 2009 | 19217687 |
nitrogen fixation and translocation in young sugarcane (saccharum officinarum l.) plants associated with endophytic nitrogen-fixing bacteria. | the tracer (15)n(2) was used to investigate sites of n(2) fixation and the possible translocation of the fixed n. young sugarcane plants (saccharum officinarum l.) from a stem cutting were exposed to (15)n(2)-labeled air in a 500 ml plastic cylinder. plants fed (15)n(2) for 7 days were grown in normal air for a further chase period. after 21 days, about half of the n originating in the stem cutting had been transported to the shoot and roots, suggesting that the cutting played a role in supplyin ... | 2009 | 21566377 |
supply and demand: sink regulation of sugar accumulation in sugarcane. | sugarcane (saccharum spp. hybrids) accumulates sucrose to high concentrations and, as a result, has been the focus of extensive research into the biochemistry and physiology of sucrose accumulation. despite this, the relationship between source leaf photosynthetic activity and sucrose accumulation in the culm sink is not well understood. the observations that photosynthetic activity declines during culm maturation in commercial cultivars and that high-sucrose-accumulating noble ancestral genotyp ... | 2009 | 19050062 |
sugarcane genes associated with sucrose content. | sucrose content is a highly desirable trait in sugarcane as the worldwide demand for cost-effective biofuels surges. sugarcane cultivars differ in their capacity to accumulate sucrose and breeding programs routinely perform crosses to identify genotypes able to produce more sucrose. sucrose content in the mature internodes reach around 20% of the culms dry weight. genotypes in the populations reflect their genetic program and may display contrasting growth, development, and physiology, all of wh ... | 2009 | 19302712 |
monodisperse lignin fractions as standards in size-exclusion analysis: comparison with polystyrene standards. | the difficulty of preparing monodisperse lignin fractions on a large scale is a limiting factor in many applications. the present paper addresses this problem by examining the properties and size-exclusion behavior of lignin isolated by the acetosolv pulping process from post-extraction crushed sugarcane bagasse. the isolated lignin was subjected to a solvent pretreatment, followed by preparative gel permeation chromatography fractionation. the fractions were analyzed by high-performance size-ex ... | 2009 | 19269646 |
biotechnological production of xylitol: enhancement of monosaccharide production by post-hydrolysis of dilute acid sugarcane hydrolysate. | dilute-acid hydrolysis pretreatment of sugarcane bagasse resulted in release of 48% (18.4 g/l) of the xylan in the hemicellulose fraction into the hydrolysate as monomeric xylose. in order to enhance the recuperation of this monomer, a post-hydrolysis stage consisted of thermal treatment was carried out. this treatment resulted in an increase in xylose release of 62% (23.5 g/l) of the hemicellulose fraction. original and post-hydrolysates were concentrated to the same levels of monomeric xylose ... | 2009 | 19214792 |
characterization of a sugarcane (saccharum spp.) gene homolog to the brassinosteroid insensitive1-associated receptor kinase 1 that is associated to sugar content. | the present article reports on the characterization of scbak1, a leucine-rich repeat receptor-like kinase from sugarcane (saccharum spp.), expressed predominantly in bundle-sheath cells of the mature leaf and potentially involved in cellular signaling cascades mediated by high levels of sugar in this organ. in this report, it was shown that the scbak1 sequence was similar to the brassinosteroid insensitive1-associated receptor kinase1 (bak1). the putative cytoplasmatic domain of scbak1 contains ... | 2009 | 19096852 |
informative genomic microsatellite markers for efficient genotyping applications in sugarcane. | genomic microsatellite markers are capable of revealing high degree of polymorphism. sugarcane (saccharum sp.), having a complex polyploid genome requires more number of such informative markers for various applications in genetics and breeding. with the objective of generating a large set of microsatellite markers designated as sugarcane enriched genomic microsatellite (segms), 6,318 clones from genomic libraries of two hybrid sugarcane cultivars enriched with 18 different microsatellite repeat ... | 2009 | 18946655 |
impact of hurricane rita storm surge on sugarcane borer (lepidoptera: crambidae) management in louisiana. | twelve thousand to 16,000 ha of louisiana sugarcane (saccharum spp.) fields were flooded by saltwater from the hurricane rita storm surge in september 2005. a four treatment, 12-replication study comparing storm surge flooded and nonflooded plant and ratoon sugarcane fields was conducted during summer 2006 to assess sugarcane borer, diatraea saccharalis (f.), pest severity, pest control actions, and soil-associated arthropod abundance and diversity. even with a significant 2.4-fold increase in t ... | 2009 | 19610419 |
isobutyl amides--potent compounds for controlling diatraea saccharalis. | a dichloromethane-methanol extract of the seeds of piper tuberculatum jacq. (piperaceae) and two isobutyl amides, 4,5-dihydropiperlonguminine (1) and pellitorine (2), which were isolated by chromatographic methods, were assayed for their lethality against the sugarcane borer diatraea saccharalis f. (lepidoptera: pyralidae). | 2009 | 18785219 |
vermistabilization of municipal sewage sludge amended with sugarcane trash using epigeic eisenia fetida (oligochaeta). | efforts have been made in this study to stabilize the sewage sludge mixed with sugarcane trash in four different proportions: 20% (t(1)); 40% (t(2)); 60% (t(3)) and 80% (t(4)), under laboratory conditions using epigeic earthworm (oligochaeta) eisenia fetida. the composting potential of worm was also evaluated in 100% sewage sludge treatment (t(5)). the changes in chemical properties of substrate was measured at the end. the vermicomposted material showed decrease in organic c (4.8-12.7%) and exc ... | 2009 | 18672324 |
consistent patterns in leaf lamina and leaf vein carbon isotope composition across ten herbs and tree species. | wide-spread post-photosynthetic fractionation processes deplete metabolites and plant compartments in (13)c relative to assimilates to varying degrees. fragmentation fractionation and exchange of metabolites with distinct isotopic signatures across organ boundaries further modify the patterns of plant isotopic composition. heterotrophic organs tend to become isotopically heavier than the putative source material as a result of respiratory metabolism. in addition fractionation may occur during me ... | 2009 | 19603469 |
novel strategy using an adsorbent-column chromatography for effective ethanol production from sugarcane or sugar beet molasses. | molasses-based distilleries generate large volumes of a highly polluted and dark brown-colored wastewater. the present work describes the way in which an adsorbent-column chromatography can effectively remove the colorant and produce biomass ethanol from sugarcane or sugar beet molasses. it was found that the color and chemical oxygen demand of the resulting wastewater was respectively reduced by approximately 87% and 28% as compared with conventional molasses fermentation. gas chromatography sh ... | 2009 | 19467586 |
screening of trichoderma isolates as a biological control agent against ceratocystis paradoxa causing pineapple disease of sugarcane. | in this study, dual culture, poison agar, and direct methods were used to assess the ability of trichoderma virens imi-392430, t. pseudokoningii imi-392431, t. harzianum imi-392432, t. harzianum imi-392433, and t. harzianum imi-392434 to control ceratocystis paradoxa, which causes the pineapple disease of sugarcane. the highest percentage inhibition of radial growth (pirg) values were observed with t. harzianum imi-392432 using two dual culture methods, 63.80% in method i and 80.82% in method ii ... | 2009 | 23983548 |
effect of sugarcane diets and a high fibre commercial diet on fresh meat and dry-cured ham quality in local caribbean pigs. | the effects sugar cane (sc) diets and a milling by product diet on meat quality characteristics of fresh meat and dry-cured ham were studied on a total of 48 creole (cr) pigs. pigs were fed with a control soya-bean meal corn diet (c1), a soya-bean meal wheat diet (c2), a sc fresh juice diet (sc-j), or a ground cane diet (sc-g). average bw gain was 657, 530, 546, and 200g/d for diets c1, c2, sc-j and sc-g, respectively. lean cuts (i.e., ham and loin) were significantly higher in sc-g than in c1 p ... | 2009 | 20416785 |
characterization of a phenazine and hexanoyl homoserine lactone producing pseudomonas aurantiaca strain pb-st2, isolated from sugarcane stem. | a novel strain of fluorescent pseudomonad (pb-st2) was isolated from surface-sterilized stems of sugarcane grown in pakistan. the bacterium was identified as pseudomonas aurantiaca on the basis of 16s rrna gene sequence analysis and results from physiological and biochemical characteristics carried out with api50 ch and qts 24 bacterial identification kits. assays using substrate specific media for enzymes revealed lipase and protease activities but cellulase, chitinase, or pectinase were not de ... | 2009 | 20075638 |
crop diversity for yield increase. | traditional farming practices suggest that cultivation of a mixture of crop species in the same field through temporal and spatial management may be advantageous in boosting yields and preventing disease, but evidence from large-scale field testing is limited. increasing crop diversity through intercropping addresses the problem of increasing land utilization and crop productivity. in collaboration with farmers and extension personnel, we tested intercropping of tobacco, maize, sugarcane, potato ... | 2009 | 19956624 |
industrial fuel ethanol yeasts contain adaptive copy number changes in genes involved in vitamin b1 and b6 biosynthesis. | fuel ethanol is now a global energy commodity that is competitive with gasoline. using microarray-based comparative genome hybridization (acgh), we have determined gene copy number variations (cnvs) common to five industrially important fuel ethanol saccharomyces cerevisiae strains responsible for the production of billions of gallons of fuel ethanol per year from sugarcane. these strains have significant amplifications of the telomeric sno and snz genes, which are involved in the biosynthesis o ... | 2009 | 19897511 |
isolation, characterization, and effect of fluorescent pseudomonads on micropropagated sugarcane. | in this study, we report on the isolation, identification, and characterization of seven fluorescent pseudomonads isolated from the roots, shoots, and rhizosphere soil of sugarcane and their impacts on the growth of sugarcane plantlets. 16s rrna gene sequence of five isolates showed close homology with pseudomonas putida, one with pseudomonas graminis, and one with pseudomonas fluorescens. physiological and biochemical characterizations were determined using api50ch and qts24 identification kits ... | 2009 | 19898541 |
natural yeast flora of different varieties of grapes used for wine making in india. | the natural saccharomyces and non-saccharomyces yeast flora present on the grape berries significantly affect wine production. six grape varieties, bangalore blue, zinfandel, cabernet, chenin blanc, sauvignon blanc and shiraz are being used in india for wine making. the yeast diversity was studied on the basis of morphological, colony, physiological characteristics and 5.8s-its sequencing of rdna of the isolates. eleven different species belonging to seven genera were identified as: candida azym ... | 2009 | 19835764 |
ethanol and xylitol production from glucose and xylose at high temperature by kluyveromyces sp. iipe453. | a yeast strain kluyveromyces sp. iipe453 (mtcc 5314), isolated from soil samples collected from dumping sites of crushed sugarcane bagasse in sugar mill, showed growth and fermentation efficiency at high temperatures ranging from 45 degrees c to 50 degrees c. the yeast strain was able to use a wide range of substrates, such as glucose, xylose, mannose, galactose, arabinose, sucrose, and cellobiose, either for growth or fermentation to ethanol. the strain also showed xylitol production from xylos ... | 2009 | 19768475 |
complete genome sequence of the sugarcane nitrogen-fixing endophyte gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus pal5 is an endophytic diazotrophic bacterium that lives in association with sugarcane plants. it has important biotechnological features such as nitrogen fixation, plant growth promotion, sugar metabolism pathways, secretion of organic acids, synthesis of auxin and the occurrence of bacteriocins. | 2009 | 19775431 |
inheritance of resistance to bacillus thuringiensis cry1ab protein in the sugarcane borer (lepidoptera: crambidae). | inheritance traits of a cry1ab-resistant strain of the sugarcane borer, diatraea saccharalis (f.) were analyzed using various genetic crosses. reciprocal parental crosses between cry1ab-susceptible and cry1ab-resistant populations, f(1) by f(1) crosses, and backcrosses of f(1) with the cry1ab-resistant population were successfully completed. larval mortality of the parental and cross-populations were assayed on cry1ab diet and bacillus thuringiensis (bt)-corn leaf tissue. maternal effects and se ... | 2009 | 19527726 |
pha(mcl) biosynthesis systems in pseudomonas aeruginosa and pseudomonas putida strains show differences on monomer specificities. | the production of pha from plant oils by pseudomonas species soil isolated from a sugarcane crop was evaluated. out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate pha. pseudomonas putida and pseudomonas aeruginosa strains produced pha presenting differences on monomer composition compatible with variability on monomer specificity of their pha biosynthesis system. the molar fraction of 3-hydroxydodecanoate detected in the pha was linearly correlate ... | 2009 | 19540884 |
artisanal alcohol production in mayan guatemala: chemical safety evaluation with special regard to acetaldehyde contamination. | there is a lack of knowledge regarding the composition, production, distribution, and consumption of artisanal alcohol, particularly in the developing world. in nahualá, an indigenous mayan municipality located in highland guatemala, heavy alcohol consumption appears to have had a significant negative impact on health, a major role in cases of violence and domestic abuse, and a link to street habitation. cuxa, an artisanally, as well as commercially produced sugarcane alcohol, is widely consumed ... | 2009 | 19729189 |
purification and characterization of pectin lyase secreted by penicillium citrinum. | the importance of various parameters such as sugarcane juice concentration, ph of the medium, and effects of different solid supports for maximum secretion of pectin lyase from penicillium citrinum mtcc 8897 has been studied. the enzyme was purified to homogeneity by sephadex g-100 and deae-cellulose chromatography. the molecular mass determined by sds-page was 31 kda. the k(m) and k(cat) values were found to be 1 mg/ml and 76 sec(-1), respectively. the optimum ph of the purified pectin lyase wa ... | 2009 | 19747102 |
a novel sugarcane-infecting mastrevirus from south africa. | the sugarcane-infecting streak viruses (sisvs) are a diverse collection of mastreviruses (family geminiviridae) within the african streak virus group. four sisvs have currently been described, including the well-characterized maize streak virus. here, we present a full annotated sequence record of an isolate of a new sisv species, saccharum streak virus (sacsv), isolated in south africa. the isolate shares less than 66% identity with any other mastrevirus, but is most closely related to urochloa ... | 2009 | 19756359 |
identification of new variants of scmv causing sugarcane mosaic in india and assessing their genetic diversity in relation to scmv type strains. | sugarcane mosaic virus (scmv) is one of the two causative viruses of mosaic in sugarcane, a sugar crop widely grown under tropical and subtropical conditions worldwide. although molecular characterization of scmv strains was reported from many countries, strains occurring in india, a major sugarcane producer have not been reported so far. twenty-six sugarcane samples represented by seven major sugarcane growing states in india and usa were subjected to reverse-transcription polymerase chain reac ... | 2009 | 19757007 |
regulation of hmgcoa reductase activity by policosanol and octacosadienol, a new synthetic analogue of octacosanol. | octacosa-10,19-dien-1-ol is a newly synthesized long-chain alcohol, an unsaturated analogue of 1-octacosanol, the major component of policosanol, the purified natural mixture of different higher aliphatic alcohols obtained from sugarcane wax. our efficient synthetic protocol (five steps with 50% overall yield) is well suited for gram scale preparations and a rapid generation of analogues with different degrees of unsaturation. beneficial effects of policosanol in the prevention of atherosclerosi ... | 2009 | 19763655 |
identification and characterization of gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc. | gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. the potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. in this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. a transposon mutant library was constructed and scree ... | 2009 | 19340412 |
ethanol production from sweet sorghum juice using very high gravity technology: effects of carbon and nitrogen supplementations. | ethanol production from sweet sorghum juice by saccharomyces cerevisiae np01 was investigated under very high gravity (vhg) fermentation and various carbon adjuncts and nitrogen sources. when sucrose was used as an adjunct, the sweet sorghum juice containing total sugar of 280 g l(-1), 3 g yeast extract l(-1) and 5 g peptone l(-1) gave the maximum ethanol production efficiency with concentration, productivity and yield of 120.68+/-0.54 g l(-1), 2.01+/-0.01 g l(-1) h(-1) and 0.51+/-0.00 g g(-1), ... | 2009 | 19375908 |
understanding the hiv epidemic in the dominican republic: a prevention success story in the caribbean? | to analyze the general dynamics and trends of the hiv epidemic in the dominican republic (dr). | 2009 | 19384103 |
production of rhamnolipids in solid-state cultivation: characterization, downstream processing and application in the cleaning of contaminated soils. | pseudomonas aeruginosa ufpeda 614 produced a rhamnolipid biosurfactant when grown on sugarcane bagasse impregnated with a solution containing glycerol. biosurfactant levels reached 40 g of rhamnolipid per kilogram of dry initial substrate after 12 days. on the basis of the volume of liquid used, the biosurfactant levels were similar to those obtained in submerged liquid culture of a medium identical to the impregnating solution. the properties of the biosurfactant were very similar to those obta ... | 2009 | 19452471 |
a preliminary study of insect fauna on pig carcasses located in sugarcane in winter in southeastern brazil. | minimum post-mortem intervals can be estimated based on analyses of the pattern of insect succession on a carcass. in order to successfully apply this approach, insect development rates must be considered with regard to local regional climatic conditions. this study is the first to analyse insect succession on carcasses decomposing in a sugarcane crop in brazil. in all seasons, chrysomya albiceps (wiedemann) (diptera: calliphoridae) and cochliomyia macellaria (fabricius) were frequent visitors d ... | 2009 | 19493195 |
enzymatic hydrolysis of steam-pretreated lignocellulosic materials with trichoderma atroviride enzymes produced in-house. | improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol process. | 2009 | 19580644 |
triticum mosaic virus: a distinct member of the family potyviridae with an unusually long leader sequence. | the complete genome sequence of triticum mosaic virus (trimv), a member in the family potyviridae, has been determined to be 10,266 nucleotides (nt) excluding the 3' polyadenylated tail. the genome encodes a large polyprotein of 3,112 amino acids with the "hall-mark proteins" of potyviruses, including a small overlapping gene, pipo, in the p3 cistron. the genome of trimv has an unusually long 5' nontranslated region of 739 nt with 12 translation initiation codons and three small open reading fra ... | 2009 | 19594313 |
experimental evidence indicating that mastreviruses probably did not co-diverge with their hosts. | despite the demonstration that geminiviruses, like many other single stranded dna viruses, are evolving at rates similar to those of rna viruses, a recent study has suggested that grass-infecting species in the genus mastrevirus may have co-diverged with their hosts over millions of years. this "co-divergence hypothesis" requires that long-term mastrevirus substitution rates be at least 100,000-fold lower than their basal mutation rates and 10,000-fold lower than their observable short-term subs ... | 2009 | 19607673 |
genetic variability of native populations of wasmannia forel (hymenoptera: formicidae) and their biogeographical implications. | wasmannia auropunctata (roger) and wasmannia rochai forel are economically important ants in the southeast and southwest regions of bahia state, brazil. randomly amplified polymorphic dna (rapd) markers were used to distinguish and analyze the genetic variability of populations of both species from ilhéus, jequié, ba and french guyana. the genetic distances among w. auropunctata and w.rochai populations (55.8-71.4%) suggest genetic differentiation among them. wasmannia auropunctata populations f ... | 2009 | 19618055 |
alkalithermophilic actinomycetes in a subtropical area of jujuy, argentina. | the objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of jujuy, argentina, characterized by sugarcane crops. laceyella putida, laceyella sacchari, thermoactinomyces intermedius, thermoactinomyces vulgaris and thermoflavimicrobium dichotomicum were isolated on the media with novobiocin, from sugar cane plants and renewal rhizospheres, and grass and wood soils. soil ph was almost neutral or lightly alkaline, except for grass soil ... | 2009 | 19623902 |
simultaneous detection and identification of four sugarcane viruses by one-step rt-pcr. | sugarcane mosaic disease (smd) caused by the sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) and sugarcane yellow leaf disease (syld) caused by the sugarcane yellow leaf virus (scylv) are the two most prevalent and economically important viral diseases of sugarcane. in this study, a one-step quadruplex reverse transcription (rt)-pcr method that employed virus-specific primers was developed for the simultaneous detection and differentiation of ... | 2009 | 19646484 |
the complete genome sequence of triticum mosaic virus, a new wheat-infecting virus of the high plains. | the genome of triticum mosaic virus (trimv), a recently discovered mite-transmitted wheat potyvirus, was sequenced, characterized, and compared to other members of the family potyviridae. trimv has a single mrna strand of 10,266 nucleotides with a predicted polyprotein consisting of 3,112 peptides. protein alignments of the coat protein demonstrate that trimv has 45.9% identity to sugarcane streak mosaic virus strain ap (scsmv-ap), but shares only 23.2% identity to wheat streak mosaic virus. alt ... | 2009 | 19649764 |
a two-dimensional electrophoretic profile of the proteins secreted by herbaspirillum seropedicae strain z78. | herbaspirillum seropedicae is an endophytic bacterium that associates with rice, sugarcane and other economically important crops. secreted proteins play a key role in the plant-bacterial interaction. using 2d electrophoresis and peptide mass fingerprint mass spectrometry, 63 protein spots representing 41 different secreted proteins were identified during growth of h. seropedicae under nitrogen-sufficient conditions. in silico analysis showed that 25.4% of the proteins had signal peptides and 15 ... | 2009 | 19664734 |
atrazine and terbuthylazine mineralization by an arthrobacter sp. isolated from a sugarcane-cultivated soil in kenya. | a tropical soil from a kenyan sugarcane-cultivated field showed a very high capability to mineralize (14)c-ring-labeled atrazine. in laboratory experiments this soil mineralized about 90% of the applied atrazine within 98 d. the atrazine-degrading microbial community was enriched in liquid cultures containing atrazine as the sole n source and 100 mgl(-1) glucose as additional c source. from the enrichment culture a bacterial strain was isolated and identified by comparative sequence analysis of ... | 2009 | 19674769 |
degradation of aflatoxin b(1) by fungal laccase enzymes. | the enzymatic degradation of aflatoxin b(1) (afb(1)) by white rot fungi through laccase production was investigated in different liquid media. a significant (p<0.0001) correlation was observed between laccase activity and afb(1) degradation exhibited by representatives of peniophora and pleurotus ostreatus cultivated in minimal salts (msm) (r=0.93) and mineral salts - malt extract (msb-meb) (r=0.77) liquid media. peniophora sp. scc0152 cultured in msb-meb liquid medium supplemented with veratryl ... | 2009 | 19683355 |
hydrolysis of cellulose derived from steam exploded bagasse by penicillium cellulases: comparison with commercial cellulase. | a complete cellulase from penicillium pinophilum was evaluated for the hydrolysis of alpha-cellulose derived from steam exploded sugarcane bagasse and other cellulosic substrates. alpha-cellulose at 1% substrate concentration was completely hydrolyzed by penicillium cellulase within 3h wherein at 10% the hydrolysis was 100% within 24 h with an enzyme loading of 10 fpu/g. the hydrolysate yielded glucose as major end product as analyzed by hplc. under similar conditions, hydrolysis of sigmacell (m ... | 2009 | 19683917 |
comparative hydrolysis and fermentation of sugarcane and agave bagasse. | sugarcane and agave bagasse samples were hydrolyzed with either mineral acids (hcl), commercial glucanases or a combined treatment consisting of alkaline delignification followed by enzymatic hydrolysis. acid hydrolysis of sugar cane bagasse yielded a higher level of reducing sugars (37.21% for depithed bagasse and 35.37% for pith bagasse), when compared to metzal or metzontete (agave pinecone and leaves, 5.02% and 9.91%, respectively). an optimized enzyme formulation was used to process sugar c ... | 2009 | 19000863 |
bioremediation of chromium(vi) contaminated soil by streptomyces sp. mc1. | this work provides quantitative information on cr(vi) reduction in soil samples by an indigenous actinomycete. streptomyces sp. mc1, previously isolated from sugarcane, has shown ability to reduce cr(vi) in liquid minimal medium. a reduction of 100 and 75% was obtained at initial cr(vi) concentrations of 5 and 50 mg l(-1), respectively, after 48 h of incubation. bioremediation ability of streptomyces sp. mc1 was assayed in soil extracts and soil samples. relative growth of streptomyces sp. mc1 w ... | 2009 | 19025876 |
bioindicator production with bacillus atrophaeus' thermal-resistant spores cultivated by solid-state fermentation. | bacillus atrophaeus' spores are used in the preparation of bioindicators to monitor the dry heat, ethylene oxide, and plasma sterilization processes and in tests to assess sterilizing products. earlier production methods involved culture in chemically defined medium to support sporulation with the disadvantage of requiring an extended period of time (14 days) besides high cost of substrates. the effect of cultivation conditions by solid-state fermentation (ssf) was investigated aiming at improvi ... | 2009 | 19039586 |
effects of different concentrations of sugarcane alcohol on food intake and nutritional status of male and female periadolescent rats. | the present study evaluated the effects of food and alcohol intake on the nutritional and metabolic status of male and female periadolescent rats submitted to single (15%) and multiple (10%, 20%, 30%) concentrations of hydroalcoholic solutions of sugar-based alcohol associated with a feed mixture. thirty-six periadolescent wistar rats were used and randomly arranged into three groups: group a (control; 0% ethanol; six males and six females), group b (15% ethanol; six males and six females), and ... | 2009 | 19251115 |
cellulase production from spent lignocellulose hydrolysates by recombinant aspergillus niger. | a recombinant aspergillus niger strain expressing the hypocrea jecorina endoglucanase cel7b was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. the spent hydrolysates served as excellent growth media for the cel7b-producing strain, a. niger d15[egi], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in st ... | 2009 | 19251882 |
inexpensive fed-batch cultivation for high poly(3-hydroxybutyrate) production by a new isolate of bacillus megaterium. | this research aimed at increasing the cell density and production of the homopolymer polyhydroxybutyrate (phb) by bacillus megaterium ba-019, using renewable and inexpensive bioresources as a substrate. a higher cell density and a greater phb production level were obtained by using sugarcane molasses and urea as carbon and nitrogen sources, respectively. the limitation of nitrogen at a c/n molar ratio of 25 resulted in enhanced cell growth and phb production in batch cultures. fed-batch cultivat ... | 2009 | 19269585 |
inoculation of sugarcane with pantoea sp. increases amino acid contents in shoot tissues; serine, alanine, glutamine and asparagine permit concomitantly ammonium excretion and nitrogenase activity of the bacterium. | pantoea sp. is an endophytic nitrogen-fixing bacterium isolated from sugarcane tissues. the aim of the present study was to determine the contents of amino acids in sugarcane as a result of inoculation of nodes and nodal roots with pantoea sp. strain 9c and to evaluate the effects of amino acids on growth, nitrogenase activity and ammonium excretion of the bacterium. content of almost all amino acids increased in 30-day-old plantlets by root inoculation. the most abundant amino acids in shoot ti ... | 2009 | 19215998 |
optimization of verticillium lecanii spore production in solid-state fermentation on sugarcane bagasse. | verticillium lecanii is an entomopathogen with high potential in biological control of pests. we developed a solid-state fermentation with sugarcane bagasse as carrier absorbing liquid medium to propagate v. lecanii spores. using statistical experimental design, we optimized the medium composition for spore production. we first used one-factor-at-a-time design to identify corn flour and yeast extract as the best carbon and nitrogen sources for the spore production of v. lecanii. then, we used tw ... | 2009 | 19221736 |
enzymatic degradation of anthracene by the white rot fungus phanerochaete chrysosporium immobilized on sugarcane bagasse. | bagasse is a by-product of sugar milling and important fuel resource for that industry. it is a fibrous, low density material with a very wide range of particle sizes and high moisture content. the goal of this study is the development of a system based on the use of the ligninolytic enzyme manganese peroxidase (mnp) produced by phanerochaete chrysosporium for the degradation of polycyclic aromatic hydrocarbons (pahs), of which anthracene was selected as an example. the white rot fungus p. chrys ... | 2009 | 18482797 |
the effectiveness of four organic matter amendments for decreasing resin-extractable cr(vi) in cr(vi)-contaminated soils. | this paper compared the effectiveness of four organic materials for decreasing the amounts of soil extractable cr(vi) in cr(vi)-contaminated soils using the dowex m4195 resin-extraction method. organic matters were added into cr(vi)-spiked soils [500 mg cr(vi)(kgsoil)(-1)] in the form of sugarcane dregs compost (scdc), cattle-dung compost (cdc), soybean meal (sbm) and rice bran (rb), in the amounts of 0, 1%, and 2% by dry weight, respectively. the results indicated that adding only 1% organic ma ... | 2009 | 18524481 |
pva-hydrogel entrapped candida guilliermondii for xylitol production from sugarcane hemicellulose hydrolysate. | viable cells of candida guilliermondii were immobilized by inclusion into polyvinyl alcohol (pva) hydrogel using the freezing-thawing method. entrapment experiments were planned according to a 2(3) full factorial design, using the pva concentration (80, 100, and 120 g l(-1)), the freezing temperature (-10, -15, and -20 degrees c), and the number of freezing-thawing cycles (one, three, and five) as the independent variables, integrated with three additional tests to estimate the errors. the effec ... | 2009 | 18633733 |
novel isolates for biological detoxification of lignocellulosic hydrolysate. | in this paper, two new strians, issatchenkia occidentalis (lj-3, cctcc m 2006097) and issatchenkia orienalis (s-7, cctcc m 2006098), isolated from different environments on solid media, were used in the detoxification process of the hemicellulosic hydrolysate of sugarcane bagasse. high-pressure liquid chromatography elution curve of uv-absorption compounds represented by acetic acid, furfural, and guaiacol (toxic compounds found in the hemicellulosic hydrolysate) showed that several chromatograp ... | 2009 | 18649037 |
performance of dairy goats fed whole sugarcane. | five lactating goats were used in a 5x5 latin square experiment to determine the effects of feeding whole sugarcane (wsc) on intake, total tract nutrient digestibilities, milk yield and milk composition. goats were fed diets containing 0, 100, 200, 300, and 400 g kg(-1) wsc and 400, 300, 200, 100, and 0 g kg(-1) tifton hay (th). intake of dry matter and neutral detergent fiber (ndf) decreased linearly (p<0.05) as the level of wsc in the diet increased. total tract nutrient digestibilities were n ... | 2009 | 18663596 |
enhanced enzymatic hydrolysis of sugarcane bagasse by n-methylmorpholine-n-oxide pretreatment. | the cellulose dissolution solvent used in lyocell process for cellulose fiber preparation, n-methylmorpholine-n-oxide (nmmo) monohydrate, was demonstrated to be an effective agent for sugarcane bagasse pretreatment. bagasse of 20wt% was readily dissolved in nmmo monohydrate at 130 degrees c within 1h. after dissolution, bagasse could be regenerated by rapid precipitation with water as a porous and amorphous mixture of its original components. the regenerated bagasse exhibited a significant enhan ... | 2009 | 18713663 |
technical viability of the production, partial purification and characterisation of inulinase using pretreated agroindustrial residues. | the present work aimed to study the viability of the use of sugarcane molasses and corn steep liquor (csl) in a sequential inulinase production performing an up-stream pretreatment of these agroindustrial residues. a sequential strategy was used applying three central composite rotatable designs (ccrds) to optimise medium composition, followed by a down-stream step. the medium containing 150 g l(-1) molasses, 50 g l(-1) csl and 6 g l(-1) yeast extract, yielded a maximum inulinase production of 1 ... | 2009 | 18820951 |
amplified fragment length polymorphisms reveal genetic differentiation among strains of xanthomonas albilineans. | xanthomonas albilineans, the causative agent of leaf scald disease (lsd), colonizes the vascular system of sugarcane (saccharum spp. hybrids). in this study x. albilineans strains from 28 countries were differentiated by using two methods of amplified fragment length polymorphism (aflp). in the manual procedure, aflp reactions were performed on 57 x. albilineans strains and after selective amplification using radiolabelled primers, the resulting products were separated using polyacrylamide gel e ... | 2009 | 18834907 |
improvement on citric acid production in solid-state fermentation by aspergillus niger lpb bc mutant using citric pulp. | citric acid (ca) production has been conducted through a careful strain selection, physical-chemical optimization and mutation. the aim of this work was to optimize the physical-chemical conditions of ca production by solid-state fermentation (ssf) using the aspergillus niger lpb bc strain, which was isolated in our laboratory. the parental and mutant strain showed a good production of ca using citric pulp (cp) as a substrate. the physical-chemical parameters were optimized and the best producti ... | 2009 | 18925364 |
semen quality in schistosoma haematobium infected men in madagascar. | the seminal vesicles and the prostate are frequently affected by egg-induced inflammation in schistosoma haematobium infected men. the objective of this study was to assess the semen quality in men with male genital schistosomiasis (mgs). the examination of the semen samples was performed in men aged 15 to 49 years living an s. haematobium endemic area in madagascar prior to anti-schistosoma treatment with praziquantel and five months later. men from the high endemic sirama sugarcane plantation ... | 2009 | 18950598 |
susceptibility of cry1ab-resistant and -susceptible sugarcane borer (lepidoptera: crambidae) to four bacillus thuringiensis toxins. | sugarcane borer, diatraea saccharalis (f.), is a primary corn stalk borer pest targeted by transgenic corn expressing bacillus thuringiensis (bt) proteins in many areas of the mid-southern region of the united states. recently, genes encoding for cry1a.105 and cry2ab2 bt proteins were transferred into corn plants (event mon 89034) for controlling lepidopteran pests. this new generation of bt corn with stacked-genes of cry1a.105 and cry2ab2 will become commercially available in 2009. susceptibili ... | 2009 | 18955062 |
engineering sugarcane cultivars with bovine pancreatic trypsin inhibitor (aprotinin) gene for protection against top borer (scirpophaga excerptalis walker). | the inhibitory activity of bovine pancreatic trypsin inhibitor (aprotinin), a natural polypeptide and a proteinase inhibitor, was demonstrated on gut proteinases of three lepidopteran borers of sugarcane using commercially available aprotinin. a synthetic gene coding for aprotinin, designed and codon optimized for better expression in plant system (shantaram 1999), was transferred to two sugarcane cultivars namely coc 92061 and co 86032 through particle bombardment. aprotinin gene expression was ... | 2009 | 18985354 |
the p0 gene of sugarcane yellow leaf virus encodes an rna silencing suppressor with unique activities. | the sugarcane yellow leaf virus (scylv) p0, a member of the highly heterologous proteins of poleroviruses, is a suppressor of posttranscriptional gene silencing (ptgs) and has additional activities not seen in other p0 proteins. the p0 protein in previously tested poleroviruses (beet western yellows virus and cucurbit aphid-borne yellows virus), suppresses local, but not systemic, ptgs induced by both sense gfp and inverted repeat gf using its f-box-like domain to mediate destabilization of the ... | 2009 | 19046592 |
lime pretreatment of sugarcane bagasse for bioethanol production. | the pretreatment of sugarcane bagasse with lime (calcium hydroxide) is evaluated. the effect of lime pretreatment on digestibility was studied through analyses using central composite design (response surface), considering pretreatment time, temperature, and lime loading as factors. the responses evaluated were the yield of glucose from pretreated bagasse after enzymatic hydrolysis. experiments were performed using the bagasse as it comes from an alcohol/sugar factory (non-screened bagasse) and ... | 2009 | 19050835 |
genetic diversity among viruses associated with sugarcane mosaic disease in tucumán, argentina. | sugarcane leaves with mosaic symptoms were collected in 2006--07 in tucumán (argentina) and analyzed by reverse-transcriptase polymerase chain reaction (rt-pcr) restriction fragment length polymorphism (rflp) and sequencing of a fragment of the sugarcane mosaic virus (scmv) and sorghum mosaic virus (srmv) coat protein (cp) genes. scmv was detected in 96.6% of samples, with 41% showing the rflp profile consistent with strain e. the remaining samples produced eight different profiles that did not ... | 2009 | 19055433 |
study of the long-term carcinogenicity potential of d-003, a mixture of high molecular weight sugarcane wax acids, in mice. | d-003, a mixture of high aliphatic primary acids purified from sugar cane wax, has shown cholesterol-lowering, anti-platelet and antioxidant effects. previous data demonstrated that d-003 was not toxic or carcinogenic when given orally to sprague-dawley rats up to 1500 mg/kg. this study investigated the potential long-term oral carcinogenicity of d-003 in a second rodent species. of1 mice of both sexes were randomized into 4 groups treated for 18 months: a vehicle control group and three groups ... | 2009 | 19056453 |
impact of 9 years of bt-maize cultivation on the distribution of maize viruses. | this study assesses the effect of bt-maize on the distribution of maize viruses. random surveys were conducted in spain between 2001 and 2006 to evaluate the occurrence of maize viruses in bt-maize cultivation areas and in areas where this crop had not been introduced. maize dwarf mosaic virus (mdmv) was the predominant virus in bt-areas, and maize rough dwarf virus (mrdv) was the most predominant one in non-bt-areas, with mrdv an emergent virus in both types of areas. a decline in the occurrenc ... | 2009 | 19067216 |
the studies on nutrient balances in crossbred cattle bulls fed chopped green sugarcane tops supplemented with some agro industrial by- products. | fifteen adult crossbred cattle bulls, 18-22 months old (mean body weight 286.33 + 9.43) were divided into 3 groups following rbd design and were fed sugarcane tops (sct) supplemented with some agro-industrial byproducts and concentrate feeds to determine nitrogen, ca and p in crossbred cattle bull. for this purpose, crossbred cattle bulls were fed for 30 days ad lib sct supplemented with wheat bran (wb), lentil chuni (lc) and wb + lc (50:50) in group i, ii, and iii respectively. the balance valu ... | 2009 | 19067220 |