Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a strain of arthrobacter that tolerates high concentrations of nitrate. | a gram-positive strain identified as arthrobacter globiformis cect 4500, tolerant to up to 1 m nitrate, was isolated from the grounds of a munitions factory. under strict aerobic conditions, this bacterium used a wide variety of c-sources to obtain the energy required for growth, which took place when the nitrate concentration in the medium was below 150 mm. cells of this bacterium growing in the absence of nitrate were seen as individual cells or forming pairs, whereas cells grown in the presen ... | 1997 | 15765585 |
| survival and phospholipid fatty acid profiles of surface and subsurface bacteria in natural sediment microcosms. | although starvation survival has been characterized for many bacteria, few subsurface bacteria have been tested, and few if any have been tested in natural subsurface porous media. we hypothesized that subsurface bacteria may be uniquely adapted for long-term survival in situ. we further hypothesized that subsurface conditions (sediment type and moisture content) would influence microbial survival. we compared starvation survival capabilities of surface and subsurface strains of pseudomonas fluo ... | 1997 | 16535578 |
| purification and characterization of two extracellular alkaline phosphatases from a psychrophilic arthrobacter isolate. | two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic arthrobacter isolate, d10. the enzymes were active over different ph ranges, used distinct substrates, and had different kinetic properties. each enzyme reacted specifically to its own antibody during immunoblot analysis. one had both monophosphatase and diesterase activities. | 1997 | 16535659 |
| stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of arthrobacter globiformis in escherichia coli. | volume 61, no. 9, p. 3209, column 2, line 23: "l22165" should read "l22516." [this corrects the article on p. 3208 in vol. 61.]. | 1996 | 16535302 |
| production of two extracellular alkaline phosphatases by a psychrophilic arthrobacter strain. | we surveyed our collection of psychrophilic bacteria to determine the types of phosphatases they produce and whether any had heat-labile activities with potential applications. assays at different temperatures showed that the activity from one isolate was optimal at 45(deg)c and decreased dramatically above 55(deg)c. this isolate, d10, had the rod-coccus morphological cycle and cell wall amino acids associated with members of the arthrobacter genus. interestingly, we found that this strain made ... | 1996 | 16535422 |
| purification, crystallization and preliminary x-ray crystal structure analysis of copper amine oxidase from arthrobacter globoformis. | beta-phenylethylamine oxidase from the gram-positive bacterium arthrobacter globoformis has been crystallized as three crystal forms. two belong to space group c2 and one to space group p2(1)2(1)2(1), respectively. the unit-cell volumes are consistent with one infunit of 70 644 da per asymmetric unit for the two monoclinic forms, and with two infunits per asymmetric unit for the orthorhombic crystals. three-dimensional intensity data have been recorded to 2.8a resolution for one of the monoclini ... | 1996 | 15299744 |
| crystallization and preliminary x-ray analysis of a hydantoinase from arthrobacter aurescens dsm 3745. | l-hydantoinase from arthrobacter aurescens dsm 3745 has been purified to homogeneity and crystallized from polyethylene glycol solutions in a form suitable for x-ray diffraction analysis. the crystals have been grown by the sitting-drop variant of the vapour-diffusion method. x-ray diffraction studies show that the crystals belong to the monoclinic space group p2(1) with a = 111.2, b = 74.4, c = 146.5 a and beta = 106.7 degrees. its asymmetric unit contains four monomers related by 222 symmetry. ... | 1996 | 15299588 |
| meloidogyne incognita infested soil amended with chicken litter. | the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ... | 1996 | 19277155 |
| [microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ... | 1996 | 9190234 |
| uptake and utilization of n-octacosane and n-nonacosane by arthrobacter nicotianae kcc b35. | arthrobacter nicotianae kcc b35 isolated from blue-green mats densely covering oil sediments along the arabian gulf coast grew well on c10 to c40 n-alkanes as sole sources of carbon and energy. growth on c20 to c40 alkanes was even better than on c10 to c18 alkanes. biomass samples incubated for 6 h with n-octacosane (c28) or n-nonacosane (c29) accumulated these compounds as the predominant constituent alkanes of the cell hydrocarbon fractions. the even chain hexadecane c16 and the odd chain pen ... | 1996 | 8849639 |
| hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a pseudomonas species. | hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera pseudomonas, moraxella, arthrobacter and aeromonas. a hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the pseudomonas and aeromonas spp. and in total soluble fractions of the arthrobacter sp. a monomeric 19-kda non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the pseudomonas species and shown to be simi ... | 1996 | 9082922 |
| the application of serological techniques to the taxonomy of arthrobacter and related organisms. | antisera were raised against rods of 17 named arthrobacter and aureobacterium strains. antigenic relationships between these strains, other soil bacteria and new arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and elisa techniques. many of the named arthrobacter species had common antigens, and there were also common antigens amongst named arthrobacter strains and many fresh arthrobacter isolates. agglutination, elisa and immunofluo ... | 1996 | 8868431 |
| cloning and sequencing of a dextranase-encoding cdna from penicillium minioluteum. | a cdna from penicillium minioluteum hi-4 encoding a dextranase (1,6-alpha-glucan hydrolase, ec 3.2.1.11) was isolated and characterized. cdna clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. southern hybridization and restriction mapping analysis of selected clones revealed four different groups of cdnas. the dextranase cdna was identified after expressing a cdna fragment from each of the isolated groups of cdna clones in the esch ... | 1996 | 8837470 |
| a phase i clinical and pharmacokinetic study of the angiogenesis inhibitor, tecogalan sodium. | tecogalan sodium is an angiogenesis inhibitor isolated from a sulfated polysaccharide produced by the bacterium arthrobacter. the antiangiogenic effect of tecogalan sodium is thought to be mediated by the inhibition of binding of basic fibroblast growth factor to cellular receptors. | 1996 | 8839904 |
| a phylogenetic study of rubrobacter radiotolerans by sequence analysis of the 16s ribosomal rna gene. | partial sequence of the 16s ribosomal rna gene of an extremely high radiotolerant bacterium, rubrobacter radiotolerans (reclassified from arthrobacter radiotolerans by chemical characteristics) was determined by pcr-amplification from a small amount of heat-lysed biomass, followed by direct sequencing of the pcr product. the sequence was aligned with seven species of arthrobacter and also with representatives of various other bacterial groups. r. radiotolerans was confirmed to be out of the arth ... | 1996 | 8843337 |
| role of conserved asn-tyr-asp-tyr sequence in bacterial copper/2,4, 5-trihydroxyphenylalanyl quinone-containing histamine oxidase. | copper amine oxidase contains a covalently bound quinonoid cofactor, 2,4,5-trihydroxyphenylalanyl quinone (tpq), which is synthesized by post-translational modification of a specific tyrosyl residue occurring in the highly conserved sequence, asn-tyr-(asp/glu)-tyr. to elucidate the role(s) of the conserved sequence in the biogenesis of tpq, each of the corresponding residues at positions 401-404 in the recombinant histamine oxidase from arthrobacter globiformis has been replaced with other amino ... | 1996 | 8798429 |
| ym-30059, a novel quinolone antibiotic produced by arthrobacter sp. | 1996 | 8823519 | |
| microbial biosensor for free fatty acids using an oxygen electrode based on thick film technology. | a microbial biosensor based on thick film technology was developed. the microorganisms, arthrobacter nicotianae, were immobilized in ca-alginate directly on the electrode surface. for the stability of the calcium alginate gel the addition of 0.5 mm cacl2 to the assay buffer was necessary. the respiratory activity of the microorganisms was monitored by oxygen consumption at -600 mv vs. ag/agcl reference electrode. the sensor was used in a batch system and was applied to the determination of free ... | 1996 | 8828165 |
| assessment of the biodegradation potential of psychrotrophic microorganisms. | bioremediation of polluted temperate and cold temperature environments may require the activity of psychrotrophic bacteria, because their low temperature growth range parallels the ambient temperatures encountered in these environments. in the present study, 135 psychrotrophic microorganisms isolated from a variety of ecosystems in canada were examined for their ability to mineralize 14c-labelled toluene, naphthalene, dodecane, hexadecane, 2-chlorobiphenyl, and pentachlorophenol. a number of the ... | 1996 | 8742353 |
| biodegradability of oxidized poly(vinyl alcohol). | poly(vinyl alcohol) dehydrogenase (pvadh) purified from pseudomonas sp. 113p3 catalyzed an oxidation of poly(vinyl alcohol) (pva) in the presence of pyrroloquinoline quinone (pqq) to give a beta-diketone structure on pva. although pvadh oxidized not only enzymatically oxidized pva but also chemically oxidized pva, pva-degrading microorganisms, pseudomonas sp. 113p3 and arthrobacter tumescens sp. 52-1 grew on the enzymatically oxidized pva, but not on the chemically oxidized pva. this suggests th ... | 1996 | 8987864 |
| distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. | mycothiol [2-(n-acetylcysteinyl)amido-2-deoxy-alpha-d-glucopyranosyl- (1-->1)-myo-inositol] (msh) has recently been identified as a major thiol in a number of actinomycetes (s. sakuda, z.-y. zhou, and y. yamada, biosci. biotech. biochem. 58:1347-1348, 1994; h. s. c. spies and d. j. steenkamp, eur. j. biochem. 224:203-213, 1994; and g. l. newton, c. a. bewley, t. j. dwyer, r. horn, y. aharonowitz, g. cohen, j. davies, d. j. faulkner, and r. c. fahey, eur. j. biochem. 230:821-825, 1995). since thi ... | 1996 | 8606174 |
| biosynthesis of topa quinone cofactor in bacterial amine oxidases. solvent origin of c-2 oxygen determined by raman spectroscopy. | resonance raman spectroscopy is an excellent technique for providing structural information on the 2,4, 5-trihydroxyphenylalanine quinone (tpq) cofactor in copper-containing amine oxidases. this technique has been used to investigate the copper- and o2-dependent biosynthesis of the tpq cofactor in phenylethylamine oxidase (peao) and histamine oxidase from arthrobacter globiformis. incubation of the holoenzyme in h218o causes frequency shifts at 1684(-26) cm-1 in peao and at 1679(-28) cm-1 in his ... | 1996 | 8617737 |
| characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probes | the structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ... | 1996 | 8688004 |
| characterization of novel mono-o-acetylated gm3s containing 9-o-acetyl sialic acid and 6-o-acetyl galactose in equine erythrocytes. | novel mono-o-acetylated gm3s, one containing 9-o-acetyl n-glycolyl neuraminic acid and another containing 6'-o-acetyl galactose, were isolated as a mixture from equine erythrocytes, and the structures were characterized by one- and two-dimensional proton nuclear magnetic resonance (nmr) and fast atom bombardment-mass spectrometry (fab-ms). the position of the o-acetyl residue was identified by the downfield shift of the methylene protons at c-9 of n-glycolyl neuraminic acid (9-o-ac gm3) and c-6 ... | 1996 | 8737247 |
| influence of chromium (vi) and acidic conditions on removal of pentachlorophenol from soil by arthrobacter strain atcc 33790. | 1996 | 8791548 | |
| 2,4-dioxygenases catalyzing n-heterocyclic-ring cleavage and formation of carbon monoxide. purification and some properties of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from arthrobacter sp. rü61a and comparison with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from pseudomonas putida 33/1. | 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (meqdo) was purified from quinaldine-grown arthrobacter sp. rü61a. it was enriched 59-fold in a yield of 22%, and its properties were compared with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) purified from pseudomonas putida 33/1. the enzyme-catalyzed conversions were performed in an (18o)o2/(16o)o2 atmosphere. two oxygen atoms of either (18o)o2 or (16o)o2 were incorporated at c2 and c4 of the respective substrates, indicating that these unusual ... | 1996 | 8856057 |
| manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2. | a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ... | 1996 | 8555170 |
| quinaldine 4-oxidase from arthrobacter sp. rü61a, a versatile procaryotic molybdenum-containing hydroxylase active towards n-containing heterocyclic compounds and aromatic aldehydes. | quinaldine 4-oxidase from arthrobacter sp. rü61a, an inducible molybdenum-containing hydroxylase, was purified to homogeneity by an optimized five-step procedure. molecular oxygen is proposed as physiological electron acceptor. electrons are also transferred to artificial electron acceptors with e'o > -8 mv. the molybdo-iron/sulfur flavoprotein regiospecifically attacks its n-heterocyclic substrates: isoquinoline and phthalazine are hydroxylated adjacent to the n-heteroatom at cl, whereas quinal ... | 1996 | 8617260 |
| endophthalmitis involving an arthrobacter-like organism following intraocular lens implantation. | 1996 | 8922827 | |
| cold shock and cold acclimation proteins in the psychrotrophic bacterium arthrobacter globiformis si55. | the psychrotrophic bacterium arthrobacter globiformis si55 was grown at 4 and 25 degrees c, and the cell protein contents were analyzed by two-dimensional electrophoresis. cells subjected to cold shocks of increasing magnitude were also analyzed. correspondence analysis of protein appearance distinguished four groups of physiological significance. group i contained cold shock proteins (csps) overexpressed only after a large temperature downshift. group ii contained csps with optimal expression a ... | 1996 | 8655472 |
| cloning and sequencing of trehalose biosynthesis genes from arthrobacter sp. q36. | a 5.1-kbp genomic dna fragment was cloned from trehalose-producing bacterium arthrobacter sp. strain q36. sequence analysis of the dna fragment revealed two open reading frames of 2325 and 1794 bp, encoding maltooligosyltrehalose synthase (trey) and maltooligosyltrehalose trehalohydrolase (trez), respectively. the 3' end of trey overlaps with the 5' end of trez by one nucleotide, and it is suggested that treyz constitutes and operon. the deduced amino acid sequences of both enzymes have several ... | 1996 | 8605217 |
| genetic characterization of site-specific integration functions of phi aau2 infecting "arthrobacter aureus" c70. | all the essential genetic determinants for site-specific integration of corynephage phi aau2 are contained within a 1,756-bp dna fragment, carried on the integrative plasmid p5510, and are shown to be functional in escherichia coli. one open reading frame, orf4, encoding a protein of 266 amino acids was shown to represent the phi aau2 integrase. the nucleotide sequence of the phi aau2 attachment site, attp, and the attb, attl, and attr sequences in the host "arthrobacter aureus" c70 were determi ... | 1996 | 8606175 |
| sarcosine oxidase contains a novel covalently bound fmn. | sarcosine oxidase from corynebacterium sp. p-1 is a heterotetrameric protein containing three different enzymes: noncovalent fad, noncovalent nad+, and covalently bound flavin which is released as 8 alpha-(n3-histidyl)riboflavin upon complete hydrolysis of the protein. the following results show that the covalent flavin is not at the fad level, as previously proposed, but it is rather as 8 alpha-(n3- histidyl)fmn coenzyme. first, no amp is released when the protein moiety is treated with phospho ... | 1996 | 8611516 |
| effects of copper-chrome-arsenate (cca) components on pcp degradation by arthrobacter strain atcc 33790. | 1996 | 8661907 | |
| morphological, physiological, and molecular characterization of actinomycetes isolated from dry soil, rocks, and monument surfaces. | in an extended study on the biodiversity of rock-dwelling bacteria, the colony and cell morphology, physiology, protein patterns, and 16s rdna sequences of 17 bacterial strains isolated from different surfaces of rocks, stones, and monuments and from various geographical locations were characterized. all except one strain, which was found to be a bacillus, were members of the order actinomycetales. the majority of the strains either were closely related to geodermatophilus obscurus, which was al ... | 1996 | 8661940 |
| moaa of arthrobacter nicotinovorans pao1 involved in mo-pterin cofactor synthesis is an fe-s protein. | moaa, involved in an early step in the biosynthesis of the molybdopterin cofactor (moco), has not yet been characterized biochemically and the reaction it catalyzes is unknown. we overexpressed moaa from pao1 of arthrobacter nicotinovorans in escherichia coli as a n-terminal fusion with either glutathione-s-transferase or a 6-histidine tag. the pao1 encoded moaa as well as the fusion proteins functionally complement e. coli moaa mutants. here we show that purified moaa contains approximately 4 m ... | 1996 | 8706892 |
| analysis of interaction between the arthrobacter sarcosine oxidase and the coenzyme flavin adenine dinucleotide by site-directed mutagenesis. | sarcosine oxidase from arthrobacter sp. te1826 (soxa) tightly binds with the coenzyme flavin adenine dinucleotide (fad). the amino-terminal region of this enzyme was recognized as a part of the fad-binding domain by homology search analysis. comparison with other structurally well-known flavoproteins suggested that the aspartate residue at position 35 (d-35) and the motif sequence (six residues at positions 12 to 17) were important for the interaction with fad. site-directed mutagenesis of each ... | 1996 | 8779579 |
| metabolism of nitrate esters by a consortium of two bacteria. | the products of condensation of organic alcohols and nitric acid are nitrate esters with the general structure c-o-no2. these products are widely employed as vasodilators and explosives, and are true xenobiotic compounds, as they do not occur in nature. we have isolated and characterized a consortium of two microorganisms, arthrobacter ilicis and agrobacterium radiobacter, that mineralized recalcitrant ethylene glycol dinitrate. the arthrobacter strain was the actual degrading microorganism, alt ... | 1996 | 9630893 |
| genetic analysis of clavibacter toxicus, the agent of annual ryegrass toxicity. | multilocus enzyme electrophoresis was used to examine the relatedness of 52 isolates of clavibacter toxicus, the agent of annual ryegrass toxicity. these included 37 western australian (wa) field isolates sampled in 3 distinct locations over a 2-year period, and 15 isolates sampled from 6 different host plant species in 3 states in australia over approximately 8 years. seventeen reference strains for the related genera curtobacterium, rhodococcus and arthrobacter were examined for comparison. th ... | 1996 | 8870638 |
| transfer of man9glcnac to l-fucose by endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae. | we have reported that transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) can be enhanced to near completion using glcnac as an acceptor in a medium containing 30% acetone (fan j-q, takegawa k, iwahara s, kondo a, kato i, abeygunawardana c, lee yc (1995) j biol chem 270: 17723-29). in this paper, we found that the endo-a can also transfer an oligosaccharide, man9glcnac, to l-fuc using man9glcnac2asn as donor substrate in a medium containing 3 ... | 1996 | 8872122 |
| the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis. | from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ... | 1996 | 9147724 |
| isolation of arthrobacter spp. from clinical specimens and description of arthrobacter cumminsii sp. nov. and arthrobacter woluwensis sp. nov. | arthrobacter spp. are very widely distributed in the environment (e.g., soil) but have not been described as causing disease in humans. over a 6-year period, two reference laboratories isolated or received 11 strains which were eventually identified as belonging to the genus arthrobacter. these strains had been initially identified as centers for disease control and prevention coryneform group b-1 and b-3 bacteria (whitishgrayish colonies of 2 mm or greater in diameter after 24 h of incubation, ... | 1996 | 8880479 |
| cloning of the penicillium minioluteum gene encoding dextranase and its expression in pichia pastoris. | the dex gene encoding an extracellular dextranase was isolated from the genomic dna library of penicillium minioluteum by hybridization using the dextranase cdna as a probe. comparison of the gene and cdna sequences revealed that the dex gene does not contain introns. amino acid sequences comparison of p. minioluteum dextranase with other reported dextranases reveals a significant homology (29% identity) with a dextranase from arthrobacter sp. cb-8. the dex gene fragment encoding a mature protei ... | 1996 | 8905923 |
| biodegradation of 4-chlorophenol via a hydroquinone pathway by arthrobacter ureafaciens cpr706. | a newly isolated arthrobacter ureafaciens, strain cpr706, could degrade 4-chlorophenol via a new pathway, in which the chloro-substituent was eliminated in the first step and hydroquinone was produced as a transient intermediate. strain cpr706 exhibited much higher substrate tolerance and degradation rate than other strains that degraded 4-chlorophenol by the hydroxylation at the second carbon position to form chlorocatechol. strain cpr706 could also degrade other para-substituted phenols (4-nit ... | 1996 | 8931337 |
| biodegradation of dimethylsilanediol in soils. | the biodegradation potential of [14c]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14co2. when 2-propanol was added to the soil as a carbon source in addition to [14c]dimethylsilanediol, the production of 14co2 increased. a method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. by t ... | 1996 | 8953708 |
| cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium sulfolobus acidocaldarius. | trehalose biosynthesis genes, trez, trex and trey, encoding maltooligosyltrehalose trehalohydrolase (trez), glycogen debranching enzyme (trex), and maltooligosyltrehalose synthase (trey) have been cloned from the thermophilic archaebacterium sulfolobus acidocaldarius atcc33909. the amino-acid sequences deduced from trez, trex and trey are composed of 556, 713 and 720 amino-acid residues, respectively. trez and trey are 33-40% homologous to the corresponding enzymes from arthrobacter sp. q36. we ... | 1996 | 8980629 |
| flagellar proteins of motile spores of actinomycetes. | flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by sds-page analysis to flagellins of cells of other bacteria. several species of actinoplanes have a major flagellar protein of subunit sizes of 42-43 kda and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kda. the flagellar protein sizes of other actinoplanetes ranged from 32-43 kda (major) and 48-58 kda (minor). antibodies formed against the 4 ... | 1996 | 8987497 |
| [microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ... | 1996 | 9190236 |
| crystallographic and fluorescence studies of ligand binding to n-carbamoylsarcosine amidohydrolase from arthrobacter sp. | crystal structures of n-carbamoylsarcosine amidohydrolase (cshase; ec 3.5.1.59) have been analyzed by x-ray diffraction methods with two different inhibitors bound to the active site at 2.28 a and 2.37 a resolution. the catalytic center of the enzyme could be identified on the basis of these structures. the four substrate binding sites are situated at the intersubunit interfaces of the compact dimers ab and cd of the homotetrameric enzyme. both inhibitors inactivate the enzyme irreversibly throu ... | 1996 | 8913306 |
| nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18. | linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ... | 1996 | 8919789 |
| lectins binding on human sperm surface increase membrane permeability and stimulate acrosomal exocytosis. | cross-linked complexes formed between certain lectins and their specific multivalent carbohydrates and glycoconjugates on the sperm surface were studied for their ability to modify sperm membrane permeability and to induce the acrosome reaction. wheat germ agglutinin (wga), concanavalin a (con a) and peanut agglutinin (pna) increased the proportions of human spermatozoa permeable to the impermeable propidium iodide (31.9 compared with 13.8%, 38.4 compared with 18.4% and 72.7 compared with 18.9% ... | 1996 | 9239678 |
| tecogalan sodium inhibits corneal neovascularization induced by basic fibroblast growth factor. | the antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. tecogalan sodium, a sulfated polysaccharide peptidoglycan complex isolated from an arthrobacter species, has been reported to inhibit angiogenesis induced by basic fibroblast growth factor (bfgf) as well as tumor angiogenesis related to kaposi's sarcoma. corneal neovascularization induced by bfgf was inhibited by tecogalan sodium in a dose-dependent manner. since bfgf is known to have a promoting effect ... | 1995 | 8927305 |
| isolation and identification of bacteria from activated sludge purifying petroleum wastewaters. | several strains growing well in minimal media with 500 and 1000 mg/l of oil or phenol as a sole carbon source were isolated from activated sludge purifying petroleum waste waters and identified. five of the best growing strains classified as arthrobacter, pseudomonas, xanthomonas and enterobacter were selected and their capacity to remove petroleum components and phenol (in the oil fraction of petrochemical waste waters) was studied. the enzymatic activity of the strains, including respiration i ... | 1995 | 8906933 |
| transformation of synechococcus with a gene for choline oxidase enhances tolerance to salt stress. | choline oxidase, isolated from the soil bacterium arthrobacter globiformis, converts choline to glycinebetaine (n-trimethylglycine) without a requirement for any cofactors. the gene for this enzyme, designated coda, was cloned and introduced into the cyanobacterium synechococcus sp. pcc 7942. the coda gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mm. consequently the cells acquired tol ... | 1995 | 8555454 |
| isolation and partial characterization of yeast mannan hydrolysing enzymes from bacterial isolates. | a range of aerobic, mesophilic bacterial strains capable of producing extracellular alpha-mannanase and alpha-mannosidase enzymes were isolated from various natural habitats using yeast cell wall material as the selective medium. five of them were capable of producing extracellular alpha-mannosidase and alpha-mannanase on yeast mannan as the sole carbon substrate, and they were tentatively identified to the genus level as arthrobacter, streptomyces, pseudomonas, bacillus and acinetobacter. among ... | 1995 | 8577261 |
| purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans. | the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ... | 1995 | 8586617 |
| a pao1-encoded molybdopterin cofactor gene (moaa) of arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein. | a gene homologous to moaa, the gene responsible for the expression of a protein involved in an early step in the synthesis of the molybdopterin cofactor of escherichia coli, was found to be located 2.7-kb upstream of the nicotine dehydrogenase (ndh) operon on the catabolic plasmid pao1 of arthrobacter nicotinovorans. the moaa protein, containing 354 amino acids, migrated on an sds-polyacrylamide gel with an apparent molecular weight of 40,000, in good agreement with the predicted molecular weigh ... | 1995 | 8588735 |
| formation of polyhydroxylated isoflavones from the soybean seed isoflavones daidzein and glycitein by bacteria isolated from tempe. | five tempe-derived bacterial strains identified as micrococcus or arthrobacter species were shown to transform the soybean isoflavones daidzein and glycitein to polyhydroxylated isoflavones by different hydroxylation reactions. all strains converted glycitein and daidzein to 6,7,4'-trihydroxyisoflavone (factor 2) and the latter substrate also to 7,8,4'-trihydroxyisoflavone. three strains transformed daidzein to 7,8,3',4'-tetrahydroxyisoflavone and 6,7,3',4'-tetrahydroxyisoflavone. in addition, t ... | 1995 | 8588745 |
| secretory overproduction of arthrobacter simplex 3-ketosteroid delta 1-dehydrogenase by streptomyces lividans with a multi-copy shuttle vector. | the gene for 3-ketosteroid delta 1-dehydrogenase (ksdd) of arthrobacter simplex was expressed in streptomyces lividans and the secreted enzyme was overproduced by using a multi-copy shuttle vector composed of pij702 and puc19. deletional analysis of the recombinant plasmid showed that the entire coding sequence of the ksdd gene was located within a 7-kb segment of the chromosomal dna obtained from the enzyme-producing strain of a. simplex. when s. lividans carrying the recombinant plasmid was gr ... | 1995 | 8590655 |
| cellular location influences copper-dependent topaquinone formation for phenylethylamine oxidase expressed in escherichia coli k-12. | arthrobacter globiformis amine oxidase produced by escherichia coli cells grown in copper-depleted media was reported to undergo activation due to formation of its topaquinone cofactor in a copper-dependent autocatalytic reaction. likewise, a mutated e. coli amine oxidase located in the cytoplasm was reported to form topaquinone autocatalytically in an edta-sensitive reaction. here we show unequivocally that formation of an amine oxidase lacking topaquinone is primarily a consequence of the loca ... | 1995 | 8522142 |
| resonance raman spectroscopy of quinoproteins. | 1995 | 8524145 | |
| purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from arthrobacter sp. q36. | arthrobacter sp. q36 produces a novel enzyme, maltooligosyl trehalose synthase, which catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-d-glucoside) by intramolecular transglycosylation. the enzyme was purified from a cell-free extract to an electrophoretically homogeneous state by successive column chromatography on sepabeads fp-da13, deae-sephadex a-50, ultrogel aca44, and butyl-toyopearl 650m. the enzyme was s ... | 1995 | 8611744 |
| purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from arthrobacter sp. q36. | a novel enzyme, maltooligosyl trehalose trehalohydrolase from arthrobacter sp. q36 was purified from a cell-free extract to an electrophoretically pure state by successive column chromatography on sepabeads fp-da13, butyl-toyopearl 650m, deae-toyopearl 650s, and toyopearl hw-55s. the enzyme specifically catalyzed the hydrolysis of the alpha-1,4-glucosidic linkage that bound the maltooligosyl and trehalose moieties of maltooligosyl trehalose. the km of the enzyme for maltosyl trehalose, maltotrio ... | 1995 | 8611745 |
| spectrophotometric detection of topa quinone. | 1995 | 8524159 | |
| cloning and nucleotide sequence of the inulin fructotransferase (dfa i-producing) gene of arthrobacter globiformis s14-3. | a gene encoding an inulin fructotransferase (dfa i-producing) [ec 2.4.1.200] from arthrobacter globiformis s14-3 was cloned and the nucleotides sequenced, for the first time. the sequence indicated that the native enzyme protein is composed of 392 amino acid residues. the native enzyme is an extracellar enzyme produced in the culture supernatant of a. globiformis s14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. the 1.5-kb dna fragment encoding the ... | 1995 | 8534968 |
| formation of trehalose from maltooligosaccharides by a novel enzymatic system. | 1995 | 8534970 | |
| limonoate dehydrogenase from arthrobacter globiformis: the native enzyme and its n-terminal sequence. | bitter limonoids in citrus juice lower the quality and value of commercial juices. limonoate dehydrogenase converts the precursor of bitter limonin, limonoate a-ring lactone, to nonbitter 17-dehydrolimonoate a-ring lactone. this enzyme was isolated from arthrobacter globiformis cells by a combination of ammonium sulfate fractionation, cibacron blue affinity chromatography and deae ion exchange hplc. using this protocol a 428-fold purification of the enzyme was obtained. gel filtration hplc indic ... | 1995 | 7546548 |
| phylogenetic analysis of the genera cellulomonas, promicromonospora, and jonesia and proposal to exclude the genus jonesia from the family cellulomonadaceae. | the 16s rrna gene sequences of eight cellulomonas species, two promicromonospora species, and jonesia denitrificans were determined, and these sequences were compared with the sequences of about 50 representatives of the arthrobacter line of descent in the order actinomycetales. we found that in spite of its current assignment to the family cellulomonadaceae, j. denitrificans branches outside the radiation of this taxon and cannot be considered a member of it. the two promicromonospora species d ... | 1995 | 7547283 |
| reclassification of micrococcus agilis (ali-cohen 1889) to the genus arthrobacter as arthrobacter agilis comb. nov. and emendation of the genus arthrobacter. | phylogenetic evidence derived from a 16s ribosomal dna analysis indicated that the type strain of micrococcus agilis, dsm 20550 (= atcc 966 = ccm 2390), is less closely related to the type species of the genus micrococcus, micrococcus luteus, than to the type species of the genus arthrobacter, arthrobacter globiformis, and related arthrobacter species. the phylogenetic position of m. agilis is supported by the presence of peptidoglycan variation a3 alpha and by the presence of mk-9(h2) as the ma ... | 1995 | 7547308 |
| molecular cloning, expression in streptomyces lividans, and analysis of a gene cluster from arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein. | the arthrobacter simplex gene coding for 3-ketosteroid-delta 1-dehydrogenase, a key enzyme in the degradation of the steroid nucleus, was cloned in streptomyces lividans. nucleotide sequence analysis revealed that the gene for 3-ketosteroid-delta 1-dehydrogenase (ksdd) is clustered with at least two more genes possibly involved in steroid metabolism. upstream of ksdd, we found a gene, ksdr, encoding a hypothetical regulatory protein that shows homologies to kdgr, the negative regulator of pectin ... | 1995 | 7596291 |
| enhanced transglycosylation activity of arthrobacter protophormiae endo-beta-n-acetylglucosaminidase in media containing organic solvents. | the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was enhanced by inclusion of organic solvents in the reaction mixture. in aqueous solution, the transglycosylation yield relative to starting substrate was 32% using man9glcnac2asn as donor and 0.5 m glcnac as acceptor. however, in the media containing 30% (v/v) acetone, dioxane, n,n-dimethylformamide, or dimethyl sulfoxide with 0.5 m glcnac as acceptor, the transglycosylation attained y ... | 1995 | 7629071 |
| synthesis of neoglycoconjugates by transglycosylation with arthrobacter protophormiae endo-beta-n-acetylglucosaminidase. demonstration of a macro-cluster effect for mannose-binding proteins. | the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) can be enhanced dramatically by inclusion of organic solvent in the reaction mixture (see accompanying article; fan, j.-q., takegawa, k., iwahara, s., kondo, a., kato, i., abeygunawardana, c., and lee, y. c. (1995) j. biol. chem. 270, 17723-17729). this finding was extended to synthesis of important intermediates for preparation of neoglycoconjugates. when 0.2 m glcnac-o-(ch2)6nh2, glcna ... | 1995 | 7629072 |
| red cells bound to influenza virus n9 neuraminidase are not released by the n9 neuraminidase activity. | influenza virus neuraminidase (na) of the n9 subtype also possesses hemagglutinin activity and the hemagglutinating, or hemabsorbing (hb), site is distinct from the catalytic site. previous results suggested that the na was binding to sialic acid on the red cell surface, but we now report that the hb receptor is not sensitive to n9 influenza neuraminidase activity. cell lines that constitutively express n9 or n2 neuraminidase have been used to further investigate the specificity of red blood cel ... | 1995 | 7645221 |
| sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5. | the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ... | 1995 | 7590299 |
| stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of arthrobacter globiformis in escherichia coli. | the gene coding for a novel esterase which stereoselectively hydrolyzes the (+)-trans (1r,3r) stereoisomer of ethyl chrysanthemate was cloned from arthrobacter globiformis sc-6-98-28 and overexpressed in escherichia coli. the cellular content of the active enzyme reached 33% of the total soluble protein in the recombinant e. coli jm105 cells and 5.6 g/liter of culture by high-density cell cultivation. the hydrolytic activity of the recombinant e. coli cells for ethyl chrysanthemate reached 605 m ... | 1995 | 7574629 |
| degradation of iprodione by a soil arthrobacter-like strain. | a bacterial strain able to transform iprodione was isolated from a fast iprodione-degrading soil by enrichment procedures. transformation was detected through 3,5-dichloroaniline production as measured by a rapid colorimetric method. the strain, ma6, was tentatively identified as an arthrobacter sp. when it was incubated with ma6 in a minimum mineral medium (ph 6.5), iprodione (8.8 mumol/liter) was transformed into two major metabolites that were identified by high-performance liquid chromatogra ... | 1995 | 7574630 |
| establishment of oil-degrading bacteria associated with cyanobacteria in oil-polluted soil. | a unique natural microbial cocktail with promising potential for remediating oil-polluted desert in the gulf region is reported. oil-degrading micro-organisms immobilized within dense cyanobacterial mats on oily coasts of the arabian gulf were successfully established in oil-contaminated sand. those micro-organisms biodegraded 50% of the oil within 10-20 weeks. nocardioforms belonging to the genus rhodococcus predominated in the first few weeks, but after 22 weeks pseudomonas spp. increased, sha ... | 1995 | 7698954 |
| use of a polymerase-chain-reaction-amplified dna probe from pseudomonas putida to detect d-hydantoinase-producing microorganisms by direct colony hybridization. | pseudomonas putida strain dsm 84 produces n-carbamyl-d-amino acids from the corresponding d-5-monosubstituted hydantoins. the sequence of the d-hydantoinase gene from this strain (genbank accession number l24157) was used to develop a dna probe of 122 base pairs (bp) that could detect d-hydantoinase genes in other bacterial genera by dna and by colony hybridization. under conditions tolerating 32% mismatch, the probe was specific for all strains that expressed d-hydantoinase activity. these incl ... | 1995 | 7766091 |
| reaction mechanism for the conversion of 5-monosubstituted hydantoins to enantiomerically pure l-amino acids. | the specific conversion of d,l-5-monosubstituted hydantoins to optically pure l-amino acids by resting cells of arthrobacter sp. dsm 7330 has been evaluated. a new nonstereoselective hydantoinase from arthrobacter sp. dsm 7330 was isolated and characterized. when whole cells were tested, the conversion of d,l-5-methylthioethylhydantoin (d,l-5-mteh) led to the optically pure intermediate d-carbamoylmethionine (d-cm) and to the optically pure amino acid l-methionine. after purification of the hyda ... | 1995 | 7785836 |
| isomerization of trans-2,delta 5-dienoyl-coa's to delta 3,delta 5-dienoyl-coa's in the beta-oxidation of delta 5-unsaturated fatty acids. | the nadph-dependent reduction pathway for the metabolism of delta 5-unsaturated fatty acids involves the isomerization of trans-2,delta 5-dienoyl-coa, initially formed from the dehydrogenation of delta 5-enoyl-coa, to isomeric delta 3,delta 5-dienoyl-coa. the latter intermediates were then isomerized to trans-2,trans-4-dienoyl-coa, which then follows the nadph-dependent pathway mediated by 2,4-dienoyl-coa reductase. the isomerization from trans-2,delta 5-dienoyl-coa to delta 3,delta 5-dienoyl-co ... | 1995 | 7819236 |
| synthesis of neoglycoproteins using oligosaccharide-transfer activity with endo-beta-n-acetylglucosaminidase. | we describe a novel method for the enzymatic synthesis of neoglycoproteins. endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) had high levels of transglycosylation activity. the enzyme activity of endo-a was markedly increased by adding 4-l-aspartyl-glycosylamine (glcnac-asn) to the reaction mixture. digesting (man)6(glcnac)2 with the enzyme in the presence of glcnac-asn gave a mixture of hydrolytic ((man)6glcnac) and transglycosylic ((man)6(glcnac)2-asn) products. by me ... | 1995 | 7852391 |
| a manganese-dependent dioxygenase from arthrobacter globiformis cm-2 belongs to the major extradiol dioxygenase family. | almost all bacterial ring cleavage dioxygenases contain iron as the catalytic metal center. we report here the first available sequence for a manganese-dependent 3,4-dihydroxyphenylacetate (3,4-dhpa) 2,3-dioxygenase and its further characterization. this manganese-dependent extradiol dioxygenase from arthrobacter globiformis cm-2, unlike iron-dependent extradiol dioxygenases, is not inactivated by hydrogen peroxide. also, ferrous ions, which activate iron extradiol dioxygenases, inhibit 3,4-dhpa ... | 1995 | 7868595 |
| copper/topa quinone-containing histamine oxidase from arthrobacter globiformis. molecular cloning and sequencing, overproduction of precursor enzyme, and generation of topa quinone cofactor. | the gene coding for histamine oxidase has been cloned and sequenced from a coryneform bacterium arthrobacter globiformis. the deduced amino acid sequence consists of 684 residues with a calculated molecular mass of 75,109 daltons and shows a high overall identity (58%) with that of phenethylamine oxidase derived from the same bacterial strain. although the sequence similarities are rather low when compared with copper amine oxidases from other organisms, the consensus asn-tyr-asp/glu sequence, i ... | 1995 | 7876243 |
| structure of simusan, a new acidic exopolysaccharide from arthrobacter sp. | simusan, a major exopolysaccharide produced by an ethanol-utilizing arthrobacter sp. strain ce-17, contains d-glucose, d-mannose, d-galactose, l-rhamnose, d-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as o-acyl groups (presumably (presumably residues of acetic and palmitic acid). on the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, smith degradation, a ... | 1995 | 7697646 |
| analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic arthrobacter isolate. | we have characterized a new psychrotrophic arthrobacter isolate which produces beta-galactosidase isozymes. when dna from this isolate was transformed into an escherichia coli host, we obtained three different fragments, designated 12, 14, and 15, each encoding a different beta-galactosidase isozyme. the beta-galactosidase produced from fragment 12 was of special interest because the protein subunit was smaller (about 71 versus 116 kda) than those typically encoded by the lacz family. the isozym ... | 1995 | 7721689 |
| active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues. | two cysteine residues (c-265 and c-318) in the putative hydrophilic regions of sarcosine oxidase were substituted by using site-directed mutagenesis. since the mutant with the c-to-s mutation at position 318 (c318s) lost the enzyme activity, c-318 (conserved among sarcosine oxidases) is most likely a part of the active site. c265s, c265a, c265d, and c265r showed nearly the same enzymatic properties as those of the wild type. however, they were much more stable than the wild type in the presence ... | 1995 | 7887617 |
| isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker. | the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ... | 1995 | 7896070 |
| development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum. | a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ... | 1995 | 8548693 |
| comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria. | nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ... | 1995 | 8590406 |
| soil and sediment bacteria capable of aerobic nitrate respiration. | several laboratory strains of gram-negative bacteria are known to be able to respire nitrate in the presence of oxygen, although the physiological advantage gained from this process is not entirely clear. the contribution that aerobic nitrate respiration makes to the environmental nitrogen cycle has not been studied. as a first step in addressing this question, a strategy which allows for the isolation of organisms capable of reducing nitrate to nitrite following aerobic growth has been develope ... | 1995 | 7487017 |
| cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from arthrobacter sp. strain 1c. | the gene coding for opine dehydrogenase from arthrobacter sp. strain 1c was cloned onto plasmid pbluescript ks(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. transformed escherichia coli cells overproduced nad+-dependent opine dehydrogenase under control of the promoter of the lac gene on pbluescript ks(-). | 1995 | 7487048 |
| membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry. | the ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. membrane hyperpolarisation in escherichia coli, pseudomonas fluorescens, enterobacter aerogenes and arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. dye uptake was variable both between species ... | 1995 | 7590182 |
| inactivation of pathogens during aerobic and anaerobic treatments at low temperatures. | 1995 | 7749283 | |
| spectroscopic studies on the mechanism of the topa quinone generation in bacterial monoamine oxidase. | electron paramagnetic resonance (epr), circular dichroism (cd), and optical absorption spectroscopies have been used to investigate the copper-dependent autoxidation process generating the 6-hydroxydopa (topa) quinone cofactor in the recombinant phenethylamine oxidase from arthrobacter globiformis. the cupric ion bound to the copper/topa quinone-less, inactive enzyme is first reduced to cu(i), as inferred from the spectroscopic features observed under strictly anaerobic conditions. cu(i) is also ... | 1995 | 7718554 |
| selective recovery of streptosporangium fragile from soil by indirect immunomagnetic capture. | a polyclonal antibody raised to streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of streptosporangium, as determined by dot immunoblotting. an indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit m-280 dynabeads. the effects of different soil blocking agents, antibody labelling concentrations and spore/dynabead capture ... | 1995 | 7496526 |
| a phylogenetic analysis of micro-organisms isolated from subsurface environments. | three methods were used to provide information on the identity and phylogenetic relatedness of 19 aerobic, chemoheterotrophic bacteria isolated from topsoil and deep subsurface sediments at a site in south carolina. these methods were (i) analysis of selected physiological traits, (ii) restriction endonuclease analysis (rea) of genomic dna, and (iii) analysis of 16s ribosomal rna sequences. when the 16s rrna sequences were compared with those for 12 standard strains, two topsoil isolates and six ... | 1995 | 7536094 |
| sulfur-specific microbial desulfurization of sterically hindered analogs of dibenzothiophene. | dibenzothiophenes (dbts) bearing alkyl substitutions adjacent to the sulfur atom, such as 4,6-diethyldibenzothiophene (4,6-dedbt), are referred to as sterically hindered with regard to access to the sulfur moiety. by using enrichment cultures with 4,6-dedbt as the sole sulfur source, bacterial isolates which selectively remove sulfur from sterically hindered dbts were obtained. the isolates were tentatively identified as arthrobacter species. 4,6-dedbt sulfone was shown to be an intermediate in ... | 1995 | 16535189 |
| nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems. | dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ... | 1995 | 16535048 |
| two-dimensional gel electrophoresis of ribosomal proteins as a novel approach to bacterial taxonomy: application to the genus arthrobacter. | ribosomal proteins from 22 strains of 15 different species belong to the genus arthrobacter were analyzed by an improved two-dimensional gel electrophoresis. electrophoretograms of ribosomal proteins from 15 type strains had species-specific patterns. similarity coefficients (sab values) of ribosomal proteins with mol. wt. of greater than about 20,000, among strains of the same species (dna relatedness values of more than 61%) were greater than 0.85, but the sab values among strains of different ... | 1995 | 8520111 |
| changes in ester-linked phospholipid fatty acid profiles of subsurface bacteria during starvation and desiccation in a porous medium. | ester-linked phospholipid fatty acid (plfa) profiles of a pseudomonas aureofaciens strain and an arthrobacter protophormiae strain, each isolated from a subsurface sediment, were quantified in a starvation experiment in a silica sand porous medium under moist and dry conditions. washed cells were added to sand microcosms and maintained under saturated conditions or subjected to desiccation by slow drying over a period of 16 days to final water potentials of approximately - 7.5 mpa for the p. aur ... | 1994 | 16349382 |