Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| detection of virulent strains of streptococcus suis type 2 and highly virulent strains of streptococcus suis type 1 in tonsillar specimens of pigs by pcr. | we developed a pcr assay for the rapid and sensitive detection of virulent streptococcus suis type 2 and highly virulent s. suis type 1 in tonsillar specimens from pigs. the pcr primers were based on the sequence of the gene encoding the ef-protein of virulent s. suis type 2 strains (mrp+ef+) and highly virulent s. suis type 1 strains (mrp(s)ef+) and of the ef protein of weakly virulent s. suis type 2 strains (mrp+ef). the latter strains give rise to larger pcr products than the virulent strains ... | 1999 | 10414368 |
| immunomagnetic isolation of streptococcus suis serotypes 2 and 1/2 from swine tonsils. | isolation of specific serotypes of streptococcus suis from the tonsils, nasal cavities, and genital tract is difficult, since low-pathogenic serotypes and untypeable strains also inhabit these sites. an immunomagnetic separation (ims) technique for the selective isolation of s. suis serotypes 2 and 1/2 was standardized. superparamagnetic polystyrene beads (immunomagnetic beads or imb) were coated with either a purified monoclonal antibody (mab) directed to a capsular sialic acid-containing epito ... | 1999 | 10449468 |
| heat-killed streptococcus suis capsular type 2 strains stimulate tumor necrosis factor alpha and interleukin-6 production by murine macrophages. | streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. since mononuclear phagocytes have been suggested to play a central role in the pathogenesis of meningitis, the objective of the present study was to evaluate the capacity of whole killed s. suis type 2 organisms to induce the release of the proinflammatory cytokines tumor necrosis factor alpha (tnf-alpha) and interleukin-6 (il-6) by murine macrophages. induction of cytokines ... | 1999 | 10456911 |
| [streptococcus suis meningitis and neurophysiology of the acoustic system. first case report from austria]. | meningitis caused by streptococcus suis was diagnosed in a 63 year old female in austria. sixteen months after onset she still suffers from ataxia, disturbance of gait and bilateral hearing impairment. being a farmer, the patient belongs to a recognized high-risk-group, as most other infected people do. to the best of our knowledge, there has been no report of a human infection by streptococcus suis in austria so far. the results of neurophysiological tests excluded a direct lesion to the cochle ... | 1999 | 10483574 |
| the cps genes of streptococcus suis serotypes 1, 2, and 9: development of rapid serotype-specific pcr assays. | we developed three type-specific pcr assays for the rapid and sensitive detection of streptococcus suis serotype 1 (plus 14), serotype 2 (plus 1/2), and serotype 9 strains in tonsillar specimens from pigs. the pcr primers were based on the sequences of type-specific capsular genes of s. suis serotype 1, 2, and 9 strains. we recently characterized a major part of the capsular biosynthesis (cps) locus of s. suis serotype 2. here we extended these studies and characterized major parts of the cps lo ... | 1999 | 10488168 |
| rapid pcr test for streptococcus suis serotype 7. | recent epidemiological studies on streptococcus suis infections in pigs indicated that, besides serotypes 1, 2 and 9, serotype 7 is also frequently associated with diseased animals. for the latter serotype, however, no rapid and sensitive diagnostic methods are available. this hampers prevention and control programs. here, we describe the development of a type-specific pcr test for the rapid and sensitive detection of s. suis serotype 7. the test is based on dna sequences of capsular (cps) genes ... | 1999 | 10499276 |
| epithelial invasion and cell lysis by virulent strains of streptococcus suis is enhanced by the presence of suilysin. | streptococcus suis is an important pathogen of pigs causing arthritis, pneumonia and meningitis and is an occupational disease of farmers and those in the meat industry. as with other streptococci, both virulent and avirulent strains of s. suis are frequently carried asymptomatically in the tonsillar crypts and nasal cavities. little is known about the process by which virulent strains cross the mucosal epithelia to generate systemic disease and whether this process requires expression of specif ... | 1999 | 10518040 |
| experimental exposure of young pigs using a pathogenic strain of streptococcus suis serotype 2 and evaluation of this method for disease prevention. | control of streptococcus suis infections and associated disease have proven to be a difficult challenge under most farm conditions. the objective of this study was to experimentally expose young pigs with a pathogenic strain of s. suis serotype 2 as a means of controlling the disease in a commercial swine farm. prior to the start of the study, the pathogenic s. suis strain responsible for mortality in the farm was identified and used to experimentally inoculate baby piglets. over a 3-week period ... | 1999 | 10534006 |
| streptococcus suis meningitis: report of a case. | a 50-year-old policeman who presented with subacute meningitis, bilateral rectus muscle palsies, dizziness and early bilateral deafness was reported. cerebrospinal fluid (csf) revealed polymorphonuclear pleocytosis with gram-positive cocci. blood and csf cultures grew streptococcus viridans which subsequently identified to be streptococcus suis. the patient improved after treatment but deafness persisted. | 1999 | 10561950 |
| hybridization analysis of the gene encoding a hemolysin (suilysin) of streptococcus suis type 2: evidence for the absence of the gene in some isolates. | a hemolysin gene was cloned from a virulent strain of streptococcus suis type 2 strain 1933. analysis of the gene and its product revealed that it is identical to a previously reported hemolysin (suilysin) of s. suis type 2. southern hybridization analysis of the digested total genomic dna from s. suis with the cloned hemolysin dna sequences as probe indicated that the hemolysin gene is present as a single copy on the genome. genomic dna of 63 isolates of s. suis encompassing all known serotypes ... | 1999 | 10564796 |
| use of ribotyping and hemolysin activity to identify highly virulent streptococcus suis type 2 isolates. | nineteen streptococcus suis type 2 isolates were evaluated for their virulence in pigs and mice. of these, seven were determined to be highly virulent in pigs on the basis of clinical sign scores and gross pathology and histopathology results. clinical sign scores correlated with gross pathology and histopathology scores at p equal to 0.004 and p equal to 0.009, respectively. the virulence of highly virulent isolates in pigs compared somewhat with virulence in mice, but the correlation was not s ... | 1998 | 9431912 |
| colonization of suckling pigs by streptococcus suis with particular reference to pathogenic serotype 2 strains. | three swine commercial farms with high mortality rates in nursery pigs due to streptococcus suis serotype 2 were studied. brain samples from diseased animals were collected for a period of 6 to 10 mo and used to isolate the strain that was responsible for the mortality (virulent strain) in each farm. tonsil swabs from piglets at 5, 10 and 15 d were taken to assess both total colonization and colonization by the virulent strain. the effect of sow vaccination against s. suis on colonization was ev ... | 1998 | 9442935 |
| production of virulence-related proteins by canadian strains of streptococcus suis capsular type 2. | the production of muramidase-released protein (mrp), extracellular protein factor (ef) and hemolysin (suilysin) by 101 canadian field strains of streptococcus suis capsular type 2 is described. most strains (72%) isolated from diseased pigs were mrp-ef- and only 1 strain was mrp+ef+. this strain was also the only 1 to produce the hemolysin. thirteen strains (15%) were mrp+ ef- and only 3 strains were mrp* ef-. all the strains isolated from clinically healthy pigs as well as a bovine and 2 human ... | 1998 | 9442945 |
| streptococcus suis serotype 2 mutants deficient in capsular expression. | streptococcus suis serotype 2 is responsible for a wide variety of porcine infections. in addition, it is considered a zoonotic agent. knowledge about the virulence factors for this bacterium is limited but its polysaccharide capsule is thought to be one of the most important. transposon mutagenesis with the self-conjugative transposon tn916 was used to obtain acapsular mutants from the virulent s. suis type 2 reference strain s735. clones were screened by colony-dot elisa with a monoclonal anti ... | 1998 | 9493370 |
| immunochemical characterization of an igg-binding protein of streptococcus suis. | several bacterial species express surface proteins with affinity for the constant region (fc) of immunoglobulin (ig) of different animal species. previous studies from our group have reported the presence of an igg-binding protein in various serotypes of streptococcus suis. this molecule was also shown to bind in a non-immune fashion chicken igy and to our knowledge this characteristic is unique. in the present study, by dot-blotting, we showed that the native protein, obtained by affinity chrom ... | 1998 | 9544779 |
| serological characterization and antimicrobial susceptibility of streptococcus suis isolates from diagnostic samples in denmark during 1995 and 1996. | at the danish veterinary laboratory streptococcus suis infections in pigs were diagnosed in 114 cases in 1995 and in 151 cases in 1996. isolates were serotyped using specific antisera against type 1 through 28 and a total of 67 cases from 1995 and 113 cases in 1996 were tested for resistance to 11 antimicrobial agents. the majority of cases were lung diseases (57%), followed by septicaemia (16%), meningitis (15%) and endocarditis (8%). almost 96% of the isolates could be typed using the 28 antis ... | 1998 | 9595627 |
| distribution of streptococcus suis capsular types in 1997. | 1998 | 9669870 | |
| recurrent septic shock due to streptococcus suis. | 1998 | 9675698 | |
| streptococcus suis serotypes associated with different disease conditions in pigs. | 1998 | 9682433 | |
| streptococcus suis and group b streptococcus differ in their interactions with murine macrophages. | streptococcus suis type 2 and group b streptococcus type iii (gbs) are important encapsulated bacterial species causing meningitis. in the present study we compared quantitatively the uptake and intracellular survival of s. suis type 2 and gbs type iii with murine macrophages in non-opsonic conditions. the role of the capsule of both pathogens was also studied using previously obtained unencapsulated isogenic mutants. encapsulated s. suis wild-type strain was practically not phagocytosed, while ... | 1998 | 9718208 |
| phylogenetic diversity of streptococcus suis strains of various serotypes as revealed by 16s rrna gene sequence comparison. | the 16s rrna gene sequences of reference strains of streptococcus suis serotypes 1-34 and 1/2 were determined. a comparative sequence analysis showed that the degree of sequence similarity between s. suis reference strains ranged from 93.94 to 100%. a dendrogram was constructed from the similarity matrix. thirty-two strains representing 32 serotypes fell into a major group divided into three clusters. the other strains, s. suis serotypes 32, 33 and 34, were more distant. biochemical characteriza ... | 1998 | 9731300 |
| 16s rdna sequence variations of some streptococcus suis serotypes. | streptococcus suis 16s rdna from selected serotypes has been sequenced and compared with the 16s rdna sequences from serotypes 1 and 2 present in genbank. after alignment the sequenced serotypes show clusters of variation. based on these clusters, a limited phylogenetic tree showing the relationships of all of the serotypes was constructed. | 1998 | 9734065 |
| use of the protective antigen of erysipelothrix rhusiopathiae in the enzyme-linked immunosorbent assay and latex agglutination. | to establish a safe and convenient serodiagnostic method for swine erysipelas, a purified protective protein antigen of erysipelothrix rhusiopathiae, which included a large amount of protective protein (64 kda protein), was used for enzyme-linked immunosorbent assay (elisa) and the latex agglutination (la) test. in the elisa, the antisera to four different serovars (1a, 2, 5 and 20) of e. rhusiopathiae exhibit a positive reaction, while antisera to other species of bacteria (listeria monocytogen ... | 1998 | 9780828 |
| prolonged persistence of an epidemic streptococcus suis strain in a closed pig population. | 1998 | 9802198 | |
| characterisation of the gene encoding suilysin from streptococcus suis and expression in field strains. | the gene encoding suilysin was cloned from streptococcus suis serotype 2 strain p1/7. analysis of the nucleotide and translated amino acid sequence confirmed suilysin to be a member of the thiol activated cytolysin group (tacy). the pneumolysin from streptococcus pneumoniae is the most closely related orthologous gene known. suilysin was overexpressed in e. coli in an active haemolytic form. a strong correlation between the presence of the sly gene and haemolytic activity in the supernatant of s ... | 1998 | 9809426 |
| trends in the resistance to antimicrobial agents of streptococcus suis isolates from denmark and sweden. | this study was conducted to determine the mic values of historical and contemporary streptoccocus suis (serotypes 2 and 7) from denmark and s. suis (serotype 2) from sweden. a total of 52 isolates originating from 1967 through 1981 and 156 isolates from 1992 through 1997 in denmark and 13 isolates from sweden were examined for their mics against 20 different antimicrobial agents. most antimicrobials were active against most isolates. a frequent occurrence of resistance to sulphamethoxazole was o ... | 1998 | 9810623 |
| simultaneous flow cytometric measurement of streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes. | a simple flow cytometric method was used to study simultaneously the phagocytosis of streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60 min of incubation, 80.2 +/- 2.8% of swine granulocytes and 77.0 +/- 2.8% of swine monocytes were shown to contain fitc-labelled s. suis serotype 2 strain 735. using the same strain, fitc-labelled bacteria were found in 95.5 +/- 3.2% ... | 1998 | 9851001 |
| isolation of streptococcus suis capsular type 3 from a young wild boar (sus scrofa) | 1998 | 9885132 | |
| virulence of streptococcus suis type 2 for mice and pigs appeared host-specific. | a murine model for streptococcus suis infection in pigs was validated by inoculating groups of 5 balb/c and 5 cf1 mice with 10(7) cfu/ml of 13 different s. suis serotype 2 strains. the pathogenicity of these strains had been established in a standardized pig model of s. suis infection using one-week-old gnotobiotic pigs. we inoculated groups of mice intraperitoneally with 4 strains that were highly virulent for pigs and belonged to the phenotype mrp+ef+, with 4 strains, that were weakly virulent ... | 1997 | 9451461 |
| effect of different diets on oral bacteria and caries activity in sprague-dawley rats. | the conventional rat model of dental caries has been used extensively to measure the contribution of various inoculated bacteria or diets to dental caries production. only limited information is available on the effect of different diets on the oral bacterial community as a whole. in the present study, using selective and non-selective media, the oral bacterial populations of 21-day-old sprague-dawley rats fed diets with commercial rat and mouse cubes (control), high-starch (56%) and sucrose (56 ... | 1997 | 9467925 |
| isolation of streptococcus suis from a young wild boar. | 1997 | 9028599 | |
| bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of streptococcus suis. | bacterial isolation from slaughtered pigs with endocarditis was carried out from 1985 to 1994. a total of 495 (0.025%) out of 2,006,127 pigs were diagnosed as having endocarditis. though bacteria were significantly isolated from 399 of the 495 pigs, bacteria could not be isolated in 96 pigs (19.4%). in 11 pigs, 2 bacterial species were isolated from heart lesion. streptococcus suis was isolated from 127 cases (25.7%), streptococcus dysgalactiae from 75 (15.2%), erysipelothrix rhusiopathiae from ... | 1997 | 9035085 |
| streptococcus suis toxic-shock syndrome and meningitis. | three cases with s. suis bacteremia and meningitis were reported. the first case was a 23-year-old butcher who was a regular drinker of alcohol for two years and developed streptococcal toxic-shock syndrome. the organism was transmitted to him through a minor cut in his right arm. the second cases was a 49-year-old female laborer who had been consuming locally produced alcohol for 20 years and developed fever and meningitis. unfortunately, she succumbed in seven days despite intensive supportive ... | 1997 | 9078819 |
| [meningitis by streptococcus suis]. | 1997 | 9101744 | |
| virulent strains of streptococcus suis serotype 2 and highly virulent strains of streptococcus suis serotype 1 can be recognized by a unique ribotype profile. | the ribotype profiles of 42 different streptococcus suis strains were studied. these strains belonged to five serotypes and differed in their virulence for pigs as well as in the expression of the muramidase-released protein and the extracellular protein factor. for the ribotyping, chromosomal dnas were digested with ecori and were hybridized with a 1,066-bp ribosomal dna probe. the hybridization patterns showed genetic heterogeneity within and between the serotypes. pathogenic strains of seroty ... | 1997 | 9114379 |
| description of an albumin binding activity for streptococcus suis serotype 2. | this study was undertaken to investigate the binding activity of streptococcus suis serotype 2 to albumin. using flow cytometry we observed a binding activity of s. suis to albumin for virulent as well as for avirulent isolates. western immunoblots analysis revealed that a 39-kda s. suis protein was responsible, at least in part, for this binding activity. this protein showed high n-terminal homology (95.6% for the first 23 residues) with a group a streptococcus glyceraldehyde-3-phosphate dehydr ... | 1997 | 9119200 |
| streptococcus suis infection complicated by purpura fulminans and rhabdomyolysis: case report and review. | streptococcus suis infection, an important zoonotic occupational disease in humans, is associated with meningitis, arthritis, and perceptive deafness. we report a case of severe s. suis infection in a previously healthy man who developed purpura fulminans and rhabdomyolysis, complications not previously reported with this disease. | 1997 | 9145747 |
| [vitamin e as a possible aid in the control of disease problems on pig farms: a field test]. | in two sow-herds problems with weaning-diarrhoea and streptococcus suis meningitis were successfully controlled by strategic use of antibiotics during the post-weaning period. in an attempt to reduce the intake of antibiotics by farm animals, the vitamin e level in the post-weaning diet was increased from 20 ie/kg to 80 ie/kg, because vitamin e is thought to increase resistance. the effect on both farms was stunning, so a small field trial was started. in this trial the higher level of vitamin e ... | 1997 | 9157616 |
| demonstration of vertical transmission of streptococcus suis in swine by genomic fingerprinting. | isolates of streptococcus suis serotype 5 collected from three sows and nine of their pigs at birth were analyzed by genomic dna fingerprinting. the cleavage patterns of dna from s. suis isolated from the sows matched the cleavage patterns of dna from s. suis isolated from their respective pigs. | 1997 | 9163491 |
| distribution of streptococcus suis capsular types in 1996. | 1997 | 9167881 | |
| cloning and characterization of a dextranase gene (dexs) from streptococcus suis. | a gene (dexs) coding for a streptococcus suis capsular type 2 dextranase (dexs) was detected in a recombinant gene library constructed in phage lambda zapii, and its nucleotide sequence was determined. sequence comparison showed that the dexs gene product had significant similarities with enzymes which hydrolyze glucose polymers. moreover, conserved amino acids that are suggested to be part of the active site of the glucosidases are also found in dexs. the dexs gene, adjacent to the gene encodin ... | 1997 | 9197542 |
| general overview of prrsv: a perspective from the united states. | four years after the report of its discovery, porcine reproductive and respiratory syndrome virus (prrsv) continues to challenge swine producers, veterinary practitioners, and animal health researchers in the united states. the prevalence of infection is high--60% to 80% of herds is a reasonable estimate--but the clinical effects of infection vary widely among farms. in many herds, infection is unapparent and productivity seemingly unaffected. some infected herds report occasional respiratory di ... | 1997 | 9220613 |
| streptococcus suis: past and present. | steptococcus suis is a gram-positive, facultatively anaerobic coccus that has been implicated as the cause of a wide range of clinical disease syndromes in swine and other domestic animals. in swine, the disease has spread worldwide but is more prevalent in countries with intensive swine management practices. the disease syndromes caused by s. suis in swine include arthritis, meningitis, pneumonia, septicaemia, endocarditis, polyserositis, abortions and abscesses. s. suis has also been implicate ... | 1997 | 9266659 |
| identification of a streptococcus suis 60-kda heat-shock protein using western blotting. | this study was initiated to investigate the presence of stress or heat shock proteins in streptococcus suis. sds-page and western blotting using polyclonal and monoclonal antibodies directed against different bacterial heat shock proteins demonstrated cross-reactivity with a protein with an apparent molecular mass of 60 kda in all s. suis serotypes tested. the 60-kda cross-reactive protein was present in virulent and avirulent strains of s. suis serotype 2 tested. a rabbit antiserum raised again ... | 1997 | 9271866 |
| microbiology and pathology of fibrinous pericarditis in danish slaughter pigs. | the prevalence of fibrinous pericarditis detected at slaughter in danish slaughter pigs is approximately 0.02%. the microbiology and pathology of this disorder was studied through 46 field cases collected at slaughter from may 1994 to august 1995. mycoplasmas (mycoplasma hyopneumoniae, m. hyosynoviae and m. hyorhinis) were isolated from the pericardium in 38 cases and from the joints in six. m. hyopneumoniae dominated with 33 isolates from the pericardium. actinobacillus pleuropneumoniae was iso ... | 1997 | 9274146 |
| vaccinator device for delivering propellant-driven aerosols of streptococcus suis bacterin into the respiratory tracts of swine. | metered-dose propellant-driven small particle aerosols of a killed whole bacterium, streptococcus suis, were produced and characterized for their aerodynamic particle sizes and antigenicity as potential respiratory mucosal vaccines against s. suis infections in swine. to facilitate the efficient delivery of such vaccine aerosols to large animals, an electro-mechanical device was developed to synchronize aerosol release to an animal's inhalation cycles. the device was tested for its capacity to d ... | 1997 | 9286040 |
| cleavage of influenza a virus h1 hemagglutinin by swine respiratory bacterial proteases. | cleavage of influenza a virus hemagglutinin (ha) is required for expression of fusion activity and virus entry into cells. extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. the relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. we examined 23 respiratory bacterial pathogens and 150 aerobic ba ... | 1997 | 9311838 |
| virulence markers of streptococcus suis type 1 and 2. | 1997 | 9331736 | |
| virulence mechanisms of streptococcus suis. | 1997 | 9331779 | |
| murine and pig models of streptococcus suis type 2 infections are incompatible. | 1997 | 9331780 | |
| immunization of pigs against streptococcus suis serotype 2 infection using a live avirulent strain. | streptococcus suis capsular type 2 is still an important cause of economic losses in the swine industry. at the present time, vaccination of pigs against this infection is generally carried out with autogenous bacterins and results are equivocal. in this study, the protective effect of a live avirulent s. suis type 2 strain (#1330) which had induced a good protection in mice, was evaluated in swine. the experiment was performed in triplicate using 4 week-old piglets. a total of 15 piglets were v ... | 1997 | 9342451 |
| [meningitis caused by streptococcus suis type 2 in an adult]. | a 54-year-old huntsman who 3 days previously had shot a wild pig, developed severe headache, nausea and vomiting over the last 10 hours. physical examination was unremarkable except for an 8 x 4 cm large reddening of the skin over the right tibia and fever (38.2 degrees c). | 1997 | 9378053 |
| characterization and protective activity of a monoclonal antibody against a capsular epitope shared by streptococcus suis serotypes 1, 2 and 1/2. | a monoclonal antibody (mab z3) was produced using balb/c mice immunized with whole cells of streptococcus suis serotype 2 reference strain s735. screening by dot-elisa showed that mab z3, of isotype igg2b, reacted only with reference strains and field isolates of s. suis serotypes 1, 2 and 1/2. the recognized epitope was demonstrated to be polysaccharide in nature by periodate oxidation, and located in the capsule, since mab z3 reacted with purified capsular material by immunoblotting and was ab ... | 1997 | 9387239 |
| the galactosyl-(alpha 1-4)-galactose-binding adhesin of streptococcus suis: occurrence in strains of different hemagglutination activities and induction of opsonic antibodies. | the occurrence of the galactose-(alpha 1-4)-galactose-specific adhesin in streptococcus suis, a pig and human pathogen causing sepsis, meningitis, and other serious infections, was studied. poly- and monoclonal anti-bodies to the purified adhesin, as well as pigeon ovomucoid, a specific probe for the adhesin activity, detected one single protein band in extracts of s. suis. the adhesin was detected in all 23 strains studied, representing pathogenic serotypes (1, 2, 4, 5, 7, 8, and nontypeable) a ... | 1996 | 8751914 |
| purification and partial characterization of a novel antibacterial agent (bac1829) produced by staphylococcus aureus ksi1829. | a novel antimicrobial agent from staphylococcus aureus ksi1829, designated bac1829, was purified by sequential steps of ammonium sulfate precipitation, sephadex g-50 gel filtration chromatography, and hydrophobic interaction chromatography. purified bac1829 has a molecular mass of 6,418 +/- 2 da. the peptide in heat stable, since full biological activity is retained after heating at 95 degrees c for 15 min, and it is destroyed by digestion with proteases. amino acid sequence analysis revealed a ... | 1996 | 8795206 |
| distribution of streptococcus suis capsular types in 1995. | 1996 | 8801025 | |
| prevalence of various phenotypes of streptococcus suis isolated from swine in the u.s.a. based on the presence of muraminidase-released protein and extracellular factor. | the present study describes the prevalence of muraminidase-released protein (mrp) and extracellular factor (ef) proteins associated with virulence of streptococcus suis serotype 2 from a collection of usa strains. sixty-six strains belonging to serotypes 1, 2, 3, 4, 7, and 10, were analyzed with a set of double antibody sandwich elisas and western blots. nineteen of 34 serotype 2 strains from cases of swine meningitis had the mrp+ef+ phenotype. five of 7 serotype 2 strains isolated from lungs ha ... | 1996 | 8825999 |
| evaluation of a pcr for detection of actinobacillus pleuropneumoniae in mixed bacterial cultures from tonsils. | a pcr for the detection of actinobacillus pleuropneumoniae was evaluated. all of 102 field, isolates of a, pleuropneumoniae reacted in the pcr by amplification of a 985 bp product. no pcr amplification product was observed when examining strains of a. ureae, a. capsulatus, a. hominis, a. equuli, a, rossii, a. suis, escherichia coli, bordetella bronchiseptica. streptococcus suis, pasteurella haemolytica, pasteurella multocida, haemophilus parasuis, haemophilus taxon minor group, haemophilus taxon ... | 1996 | 8828126 |
| characterization of virulence of the streptococcus suis serotype 2 reference strain henrichsen s 735 in newborn gnotobiotic pigs. | strain henrichsen s 735 (nctc 10234) of streptococcus suis serotype 2 reference and three other such strains (strains s 4005, s 3921 and t 141) were tested for virulence by inoculating pigs intranasally and intravenously. the taxonomical properties of each strain were determined. phenotypes were determined by western blotting based on mrp and ef protein expression and genotypes were determined by southern hybridization analysis of the mrp and epf genes. reference strain s 735 and strain s 3921 p ... | 1996 | 8828129 |
| in vitro activity of ceftiofur and its primary metabolite, desfuroylceftiofur, against organisms of veterinary importance. | ceftiofur (xnl) and its primary metabolite, desfuroylceftiofur (dxnl), were evaluated for in vitro activity against 539 isolates from veterinary sources. actinobacillus pleuropneumoniae, pasteurella spp., haemophilus somnus, salmonella spp., escherichia coli, staphylococci, and streptococci were tested. overall, xnl and dxnl were equivalent in activity against the gram-negative organisms with all minimum inhibitory concentrations (mics) within 1 serial dilution. against the staphylococci, mic di ... | 1996 | 8844576 |
| role of capsular sialic acid in virulence and resistance to phagocytosis of streptococcus suis capsular type 2. | streptococcus suis capsular type 2 has a capsule rich in sialic acid (nana). sialic acid, known to be an antiphagocytic factor for many bacterial species, inhibits the activation of the alternative complement pathway. the role of capsular nana in virulence, resistance to phagocytosis and intracellular survival of s. suis capsular type 2 was evaluated. in general, a low concentration of nana was observed for all the s. suis strains tested. in addition, no difference could be found in nana concent ... | 1996 | 8856318 |
| susceptibility to antimicrobial agents of streptococcus suis capsular type 2 strains isolated from pigs. | the minimal inhibitory concentrations (mics) for thirty-three epidemiologicaly unrelated clinical isolates of streptococcus suis capsular type 2 were determined in relation to ampicillin, ampicillin-sulbactam, amoxicillin, clavulanate-amoxicillin, penicillin g, cephalexin, gentamicin, streptomycin, erythromycin, tylosin and doxycycline, using the microtitre broth dilution procedure described by the u.s. national committee for clinical laboratory standards (nccls). gentamicin was the most active ... | 1996 | 8861871 |
| protection of experimentally infected pigs by suilysin, the thiol-activated haemolysin of streptococcus suis. | three groups of three pigs were vaccinated either with vaccine vac-sly, containing purified suilysin derived from streptococcus suis strain p1/7 (serotype 2), or with vaccine vac-scf, containing most of the other extracellular antigens produced by strain p1/7 (but essentially free from suilysin), or with a placebo vaccine. the pigs were vaccinated twice at four weeks and six weeks of age and were challenged intravenously with s suis strain p1/7 at eight weeks of age. on the day of challenge, onl ... | 1996 | 8883345 |
| the gal alpha 1-4 gal-binding adhesin of streptococcus suis, a gram-positive meningitis-associated bacterium. | streptococcus suis causes septicaemia and meningitis in pigs and occasionally in humans. a major galactose-inhibitable adhesin recognizing the blood group p-related disaccharide gal alpha 1-4 gal beta 1--present in the gbo3 glycolipid was identified in s. suis. two variant adhesins, inhibitable by galactose and n-acetylgalactosamine (type pn) or galactose only (type po) both preferred the disaccharide in terminal position. the hydrogen bonding patterns were determined using deoxy and other deriv ... | 1996 | 8895773 |
| species selectivity of new siderophore-drug conjugates that use specific iron uptake for entry into bacteria. | siderophores selectively bind ferric iron and are involved in receptor-specific iron transport into bacteria. several types of siderophores were synthesized, and growth-promoting or inhibitory activities when they were conjugated to carbacephalosporin, erythromycylamine, or nalidixic acid were investigated. overall, 11 types of siderophores and 21 drug conjugates were tested against seven different bacterial species: escherichia coli, bordetella bronchiseptica, pasteurella multocida, pasteurella ... | 1996 | 8913474 |
| detection of antibodies against streptococcus suis capsular type 2 using a purified capsular polysaccharide antigen-based indirect elisa. | in the present study a purified capsular polysaccharide antigen-based indirect elisa (cps-elisa) to detect antibodies against streptococcus suis capsular type 2 was developed and compared with a whole cell antigen-based elisa (wca-elisa). the wca-elisa presented a very low specificity when rabbit antisera to other capsular types were tested. most of these cross-reactions were due to common proteins. the standardized cps-elisa gave satisfactory results using a concentration of 0.1 micrograms/well ... | 1996 | 8914256 |
| comparative preparation methods of sialylated capsule antigen from streptococcus suis type 2 with type specific antigenicity. | the capsular polysaccharide (cps) of streptococcus (s.) suis type 2 was isolated from a type strain of s. suis nctc 10234 by three different preparative methods: (a) lysozyme treatment method, (b) autoclave extraction method, and (c) hcl-extraction method. the structural characteristics of the three cps (cps-a, b and c) were examined by gel permeation chromatography, reactivity against rabbit antiserum and proton-nuclear magnetic resonance (1h-nmr). n-acetylneuraminic acid (neuac) residues as si ... | 1996 | 8915993 |
| binding properties of streptococcus suis for immunoglobulin g and other plasma proteins. | immunoglobulin g (igg) binding proteins on the surface of streptococcus suis could be readily detected by direct cultivation of the bacteria on nitrocellulose membranes and subsequent treatment of the membranes with human igg. among the 75 s. suis isolates tested two cultures (s. suis p43, s. suis p143) caused a blue colouration of the membranes indicating igg binding activities. the igg binding proteins could be solubilized by heat treatment of the bacteria at an acid ph and also by mutanolysin ... | 1996 | 8921739 |
| infective endocarditis due to streptococcus suis serotype 2. | 1996 | 8922583 | |
| streptococcus suis serotypes 7, 8 and 14 from diseased pigs in scotland. | 1996 | 8923718 | |
| mutants of streptococcus suis types 1 and 2 impaired in expression of muramidase-released protein and extracellular protein induce disease in newborn germfree pigs. | the contribution of muramidase-released protein (mrp) and extracellular factor (ef) to the virulence of streptococcus suis type 1 and 2 infections was studied. for that aim, we constructed mutants of s. suis types 1 and 2 by inactivating the genes encoding mrp and ef. moreover, we changed a type 2 strain producing the 110-kda ef protein into a strain producing a modified protein (ef*) of increased molecular mass. the chromosomally located mrp and epf genes were inactivated by replacement recombi ... | 1996 | 8926123 |
| streptococcus suis serotype 14 as a cause of pig disease in the uk. | 1996 | 8931303 | |
| surface location of hpr, a phosphocarrier of the phosphoenolpyruvate: sugar phosphotransferase system in streptococcus suis. | hpr is a low-molecular-mass phosphocarrier protein of the bacterial phosphoenolpyruvate (pep): sugar phosphotransferase system (pts) found in the cytoplasm or associated with the inner surface of the cytoplasmic membrane. treatment of streptococcus suis cells with a sorvall omnimixer, a technique used to extract cell surface components, resulted in the extraction of a major protein with a molecular mass of 9 kda. several lines of evidence suggested that this protein was hpr: (i) the s. suis prot ... | 1996 | 8936310 |
| [streptococcus suis l3-l4 spondylodiscitis in a butcher]. | 1996 | 8942947 | |
| interpretive criteria for antimicrobial susceptibility testing of ceftiofur against bacteria associated with swine respiratory disease. | ceftiofur, an extended-spectrum cephalosporin, is active against a variety of animal pathogens, including organisms associated with swine respiratory disease. however, minimum inhibitory concentration (mic) breakpoint and disk diffusion interpretive criteria have not been established for swine pathogens. susceptibility tests were performed by broth microdilution mic and disk diffusion methods on 246 bacterial species that cause swine respiratory disease. ceftiofur was active against salmonella s ... | 1996 | 8953533 |
| multiple serotypes and strains of streptococcus suis in naturally infected swine herds. | 1996 | 9026068 | |
| evaluation of antibiotics for the elimination of the tonsillar carrier state of streptococcus suis in pigs. | seventy clinically normal 13-day-old crossbred pigs from 10 litters from a streptococcus suis-infected herd were randomly assigned by litter and weight to 7 groups of 10 pigs each to determine whether different antibiotic regimens would eliminate the tonsillar carrier state of s. suis. six antimicrobial regimens were tested: penicillin intramuscularly (im) once daily (s.i.d.) for 3 consecutive days; penicillin im s.i.d. for 5 consecutive days; ampicillin im s.i.d. for 5 consecutive days; ampicil ... | 1996 | 9026083 |
| growth and microbial flora of nonmedicated, segregated, early weaned pigs from a commercial swine operation. | to determine whether segregated, early weaned pigs have better growth performance and different microbial flora than those pigs raised on-site. | 1996 | 8617629 |
| [streptococci or slurry pit ventilation?]. | this article describes a case of disturbed behaviour in weaners resulting in mutilation. the mutilations mainly consisted of ear biting and ear necrosis. during some periods all weaners were affected. deaths occurred as a result of sepsis and meningitis, caused by streptococcus suis type 1. there were many runts. the stable was as follows: the sections contained 160 weaners; the floor consisted of plastic slats with a 1-metre-deep slurry pit. the weaners were medicated extensively for the strept ... | 1996 | 8685881 |
| in vitro phagocytosis and survival of streptococcus suis capsular type 2 inside murine macrophages. | in this study, data on phagocytosis of streptococcus suis and its survival inside macrophages are presented. mouse peritoneal macrophages were incubated in the presence of one of five different strains of s. suis capsular type 2: a virulent wild-type strain (1591), a non-capsulated non-virulent mutant strain (m2), a poorly capsulated non-virulent mutant strain (m42), a non-virulent capsulated strain (1330), and the wild-type reference (virulent) strain s735. opsonized or non-opsonized bacteria w ... | 1996 | 8704963 |
| an enzyme-linked immunosorbent assay (elisa) for the detection of antibody against streptococcus suis type 2 in infected pigs. | an elisa test for the detection of antibody against s. suis type 2 in pigs was developed and applied to field sera. the best sensitivity and specificity of elisa were obtained when a purified polysaccharide antigen was used. it showed no cross reaction with sera against other serotypes of s. suis and other pathogenic bacteria. a total of 264 sera were collected from 20 pig farms and examined with the antibody against s. suis type 2. in the affected farms, 17.0% of pigs tested were positive, 9.8% ... | 1996 | 8741273 |
| streptococcus suis meningitis: first case reported in quebec. | very few streptococcus suis infections in humans have been reported in canada, although the condition is frequent in pigs. meningitis, often accompanied by severe hearing loss, is the most common clinical manifestation. the disease is an occupational illness affecting persons in contact with pigs and may be underdiagnosed because of misidentification of the responsible bacterium. since quebec is the leading province for swine production in canada, physicians and microbiologists should be aware o ... | 1996 | 22514459 |
| recovery from bilateral vestibular failure: implications for visual and cervico-ocular function. | we report a patient who sustained severe bilateral labyrinthine lesions during streptococcus suis meningitis but considerably recovered vestibular function over a 7 month period. this unique case allowed us to examine the cervico-ocular reflex (cor) and visual function at various levels of activity of his vestibular system. the slow phase cor, elicited by trunk oscillation (0.2 hz) with the head earth-stationary, was negligible immediately after the acute vestibular loss but rose to a gain of 0. ... | 1995 | 8749174 |
| influence of recombinant bovine interleukin-1 beta and interleukin-2 in pigs vaccinated and challenged with streptococcus suis. | an experiment was conducted to determine the adjuvanticity of recombinant bovine il-1 beta (rboil-1 beta) and recombinant bovine il-2 (rboil-2) administered in conjunction with a single streptococcus suis vaccination in pigs. sixty 4-week-old pigs were allotted to eight groups: nonvaccinated controls; vaccinated controls; rboil-beta at 0.1, 1, and 10 micrograms kg-1; rboil-2 at 2.5, 25, and 250 micrograms kg-1. all pigs (except nonvaccinated controls) were vaccinated on day 0 with a commercial s ... | 1995 | 7747410 |
| use of multiscreen plates for the preparation of bacterial dna suitable for pcr. | a rapid and inexpensive method for isolating bacterial dna for use in pcr is described. the method is based on the guanidinium thiocyanate (guscn)-lysis method of boom et al. (j. clin. microbiol. 28:495-503, 1990) and enables a multiple of 96 samples to be prepared in only one hour. we use multiscreen plates and a vacuum manifold from millipore. clinical samples are lysed and washed in the wells of a multiscreen plate, and dna is eluted in a standard microplate. purified dna was recovered with h ... | 1995 | 8527150 |
| production of suilysin, the thiol-activated haemolysin of streptococcus suis, by field isolates from diseased pigs. | 1995 | 8533226 | |
| streptococcus suis as threat to pig-farmers and abattoir workers. | 1995 | 8551847 | |
| influence of dietary beta-glucan on growth performance, nonspecific immunity, and resistance to streptococcus suis infection in weanling pigs. | three experiments, using 344 pigs, were conducted to evaluate the influence of beta-glucan on growth performance, neutrophil and macrophage function, haptoglobin production, and resistance to streptococcus suis challenge in weanling pigs. in exp. 1, 144 pigs were used to evaluate the influence of .1% dietary beta-glucan in a soybean meal- or milk protein-based diet on growth performance and neutrophil function. pigs fed beta-glucan from d 7 to 14 after weaning had lower adfi (p < .01) and, altho ... | 1995 | 8586593 |
| high-efficiency transformation and gene inactivation in streptococcus suis type 2. | an efficient electrotransformation system for streptococcus suis type 2 is described. it is demonstrated that vectors based on the broad-host-range plasmid pwvo1 replicate in s. suis type 2. transformation efficiencies of about 10(7) transformants per micrograms of plasmid dna could be obtained. derivatives of plasmid pbr322 containing s. suis chromosomal dna did not replicate but integrated into the chromosome. southern hybridization analysis revealed that double as well as single cross-over in ... | 1995 | 7894710 |
| characterization of streptococcus suis capsular type 2 haemolysin. | the production of a haemolysin by streptococcus suis capsular type 2 was investigated. human group o erythrocytes were the most susceptible, followed by horse, sheep, cow and pig red blood cells, which exhibited similar susceptibilities; rabbit erythrocytes were the least susceptible. the haemolysin was produced at the end of the exponential growth phase. the toxin described in this paper was purified by affinity chromatography using a thiopropyl-sepharose 6b column. it is an extracellular prote ... | 1995 | 7894711 |
| streptococcus suis infection in hong kong. | twenty-five patients were admitted to two hospitals in hong kong for streptococcus suis infection between 1984 and 1993. among them, 15 (60%) had an occupational exposure to pigs or pork, and four had a clear history of skin injury up to 16 days before admission. examination of the cerebrospinal fluid of 21 patients confirmed the presence of meningitis in every case; the remaining four patients who did not have lumbar punctures had each presented with arthritis, bronchopneumonia, endocarditis an ... | 1995 | 7894987 |
| streptococcus suis meningitis in an italian blood donor. | 1995 | 7475756 | |
| distribution of serotype, virulence markers and further characteristics of streptococcus suis isolates from pigs. | this study was designed to comparatively investigate 28 s. suis cultures isolated from various pathological processes of pigs. all cultures could be identified biochemically and most of the cultures could be serogrouped with specific antiserum against lancefield's serogroup d. serotyping of the s. suis isolates mainly revealed capsular types 2, 1, and 22. in addition, part of the cultures, mostly those of serotype 2, reacted with monoclonal antibodies to the virulence protein muraminidase-releas ... | 1995 | 7483907 |
| comparison of mics of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the united states, canada, and denmark. | the mics of ceftiofur and other antimicrobial agents, tested for comparison, for 515 bacterial isolates of pigs from the united states, canada, and denmark with various diseases were compared. the organisms tested included actinobacillus pleuropneumoniae, escherichia coli, pasteurella multocida, salmonella choleraesuis, salmonella typhimurium, streptococcus suis, streptococcus dysgalactiae subsp. equisimilis, streptococcus equi subsp. equi, and streptococcus equi subsp. zooepidemicus. in additio ... | 1995 | 7494042 |
| streptococcus pneumoniae types 19a and 19f and streptococcus suis capsular type 8 share common capsular epitopes. | two monoclonal antibodies (mabs) to streptococcus pneumoniae types 19a and 19f were tested with the 35 reference strains and 334 field strains of streptococcus suis by dot blotting. both mabs reacted with the capsular type 8 reference strain, and one reacted with 69% and one reacted with 100% of 81 s. suis capsular type 8 field strains tested. epitopes recognized by both mabs are capsular in origin. | 1995 | 7494057 |
| purification of a galactosyl-alpha 1-4-galactose-binding adhesin from the gram-positive meningitis-associated bacterium streptococcus suis. | streptococcus suis causes meningitis, sepsis, and other serious infections in newborn and young pigs and in adult humans. the gal alpha 1-4gal-binding adhesin of s. suis was purified to homogeneity by ultrasonic treatment, fractional ammonium sulfate precipitation, and preparative polyacrylamide gel electrophoresis. pigeon ovomucoid, a glycoprotein with gal alpha 1-4gal terminals, was used to detect the adhesin by blotting. the purified adhesin appeared as single band of an apparent size of 18 k ... | 1995 | 7499414 |
| immunoblot analysis of bacterial polysaccharides: application to the type-specific polysaccharides of streptococcus suis and streptococcus agalactiae. | a method for the immunoblot analysis of the type-specific capsular polysaccharides of streptococci was developed. the capsular polysaccharides were extracted by sonication and subjected to polyacrylamide gel electrophoresis in the absence of sodium dodecyl sulphate (sds). after electrophoresis the polysaccharides were transferred to charged pvdf-n membranes and probed with the type specific antibodies. a characteristic ladder-like pattern of polysaccharide bands specific for the serotype (1, 2, ... | 1995 | 7499882 |
| detection of genomic heterogeneity in streptococcus suis isolates by dna restriction fragment length polymorphisms of rrna genes (ribotyping). | whole-cell chromosomal digests of 54 isolates of streptococcus suis encompassing all known serotypes from a geographically varied collection were examined by psti restriction fragment length polymorphisms and then hybridized with a digoxigenin-11-dutp-labeled cdna probe transcribed from a mixture of 16s and 23s rrnas from escherichia coli mre600. the hybridization patterns showed genetic heterogeneity within and between s. suis serotypes. most isolates (87%) representing 28 serotypes contained a ... | 1995 | 7540630 |
| agglutination of streptococcus suis by sialic acid-binding lectins. | the 35 streptococcus suis capsular-type reference strains as well as 45 field strains of type 2 were tested with sialic acid-binding lectins from sambucus nigra (sna i), triticum vulgaris, maackia amurensis, homarus americanus, and limax flavus. only types 1, 1/2, 2, 14, 15, and 16 agglutinated with sna i and/or the t. vulgaris lectin. all field strains agglutinated only with sna i. reaction with sna i was probably due to the sialic acid moiety since it disappeared after sialidase treatment. the ... | 1995 | 7559986 |
| properties of a streptococcus suis isolate of serotype 2 and two capsular mutants. | encapsulation is thought to be a critical virulence factor of streptococcus suis. in the present study two capsular type 2 mutants of s. suis (m42 and m2) and their s. suis parent strain (89-1591) were further characterized. all three cultures reacted with group d specific antiserum whereas parent strain 89-1591 and mutant m42 but not mutant m2 reacted with specific antiserum against capsular type 2. both mutants had higher surface hydrophobicity and showed an increased adherence to human epithe ... | 1995 | 7571366 |