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microbiology of mediastinitis.to study the microbiologic and clinical characteristics of patients with mediastinitis.19968572845
identification and discrimination of oral asaccharolytic eubacterium spp. by pyrolysis mass spectrometry and artificial neural networks.curie-point pyrolysis mass spectra were obtained from 29 oral asaccharolytic eubacterium strains and 6 abscess isolates previously identified as peptostreptococcus heliotrinreducens. pyrolysis mass spectrometry (pyms) with cluster analysis was able to clarify the taxonomic position of this group of organisms. artificial neural networks (anns) were then trained by supervised learning (with the back-propagation algorithm) to recognize the strains from their pyrolysis mass spectra; all eubacterium ...19968574131
bacteriology and beta-lactamase activity in acute and chronic maxillary sinusitis.to assess the bacteriology and beta-lactamase enzyme activity in aspirates of 10 acutely and 13 chronically inflamed maxillary sinuses.19968600928
aerobic and anaerobic microbiology of infected hemorrhoids.to study the aerobic and anaerobic microbiology of infected hemorrhoids.19968607502
[antimicrobial activity of cefodizime against clinical isolates].in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ...19968986558
associations of endodontic symptoms and signs with particular combinations of specific bacteria.significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...19969206427
variations in the susceptibilities of components of the endodontic microflora to biomechanical procedures.the role of the endodontic microflora in pulpal disease and in endodontic treatment failures is well established. thus the need for effective microbial control is one of the important justifications for biomechanical procedures. however the efficacy of this stage of treatment is dependent upon the vulnerability of the involved species, which may not be uniform. the aim of this study was to investigate variations in the susceptibilities of members of the root canal microflora to routine biomechan ...19969206439
the antimicrobial activity of endodontic sealers to anaerobic bacteria.the effect of five root canal sealers and two root canal dressing materials on the growth of three anaerobic bacteria associated with endodontic infections was determined using the agar diffusion inhibitory test. samples of the following endodontic sealers (apexit, ketac-endo, roth sealer, sealapex and tubliseal) and root canal dressing materials (pulpdent and root-cal) were incubated for 48 h with the following anaerobic bacteria: capnocytophaga ochracea, porphyromonas gingivalis and peptostrep ...19969206446
matrix metalloproteinases-1, -3 and -8 and myeloperoxidase in saliva of patients with human immunodeficiency virus infection.human immunodeficiency virus (hiv)-seropositive patients have frequently severe gingival inflammation and/or attachment loss. in addition many infectious diseases affect their periodontium with varying clinical manifestations. matrix metalloproteinases seem to play a key role in physiological periodontal remodelling and pathological tissue destruction. the aim of the present study was to characterize the presence, molecular forms, cellular sources, activities, and relative amounts of fibroblast- ...19969171509
macrolides resistance of common bacteria isolated from taiwan.to determine the susceptibility to macrolides of common pathogenic bacteria isolated from taiwan, the in vitro activities of erythromycin, roxithromycin, azithromycin, clarithromycin, and dirithromycin were tested against 492 clinical isolates of eight different bacteria, collected from the national taiwan university hospital. the results showed high minimum inhibitory concentrations (mics) against most of the tested bacteria. the mic90s for staphylococcus aureus (both methicillin-resistant and ...19959407220
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
phylogenetic analysis of ruminococcus flavefaciens, the type species of the genus ruminococcus, does not support the reclassification of streptococcus hansenii and peptostreptococcus productus as ruminococci.the 16s rrna gene sequence of the type strain of ruminococcus flavefaciens, the type species of the genus ruminococcus, was determined by pcr direct sequencing. a comparative sequence analysis showed that r. flavefaciens is phylogenetically related to a small cluster (cluster iv of collins et al. [m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994]) of organisms which include ...19958590686
antimicrobial and toxic effects of established and potential root canal irrigants.three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ...19958596073
pet findings in a brain abscess associated with a silent atrial septal defect.brain abscesses are classical complications of congenital heart disease (chd) in children and adolescents. this association is rarely observed in adults. we report a 46-year-old man presenting a fronto-parietal abscess associated with an asymptomatic atrial septal defect. positron emission tomography (pet) study revealed high uptake of l-[methyl-11c]methionine ([11c]methionine) and 2-[18f]fluoro-2-deoxy-d-glucose (fdg) around the brain abscess. we suggest (1) to exclude a silent cardiac malforma ...19958599907
treatment of peptostreptococcus micros endocarditis with teicoplanin. 19958562764
a case of anaerobic pericarditis. 19958562770
surgical sepsis: constancy of antibiotic susceptibility of causative organisms.it is well documented that antibiotic therapy exerts selective pressure on bacteria. conversion of bacteria from susceptible to resistant to antibiotics has been observed often during antimicrobial therapy. it has been postulated that human intestinal reservoirs facilitate communication of transposons that can transfer resistance determinants among various bacterial species.19957740421
adherence of bacteria to intraocular lenses: a prospective study.the study was designed to investigate the bacterial flora of the operating field during routine cataract surgery and the source of intraocular lens contamination during the surgery.19957742281
bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from peptostreptococcus magnus.to determine the role of cysteine conjugate beta-lyase (beta-lyase) in the metabolism of mutagenic nitropolycyclic aromatic hydrocarbons, we determined the effect of beta-lyase on the mutagenicities and dna binding of cysteine conjugates of 4,5-epoxy-4,5-dihydro-1-nitropyrene (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-nitropyrene (1-np 9,10-oxide), which are detoxified metabolites of the mutagenic compound 1-nitropyrene. we purified beta-lyase from peptostreptococcus magnus gai0663, since p. ...19958526486
structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins.three-dimensional structures are now available for several immunoglobulin binding domains from bacterial proteins a, g, and l. x-ray diffraction and nmr experiments on complexes of these domains with portions of immunoglobulins have revealed common structural themes used in these interactions. these data expand our understanding of structure/function relationships in these molecular recognition processes and provide the basis for rational design of artificial immunoglobulin-binding molecules.19958528763
salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment.the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ...19958530742
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
a study of the anaerobic bacterial flora of the female genital tract in health and disease.semi-quantitative and qualitative bacterial assessment of the vaginal and cervical flora of a total of 202 women was carried out over a period of six months to determine the bacterial flora in three groups of women and changes caused by prior use of antibiotics. the number was made up of 32 healthy volunteers, 80 women with gynaecological problems and 90 women with gynaecological infections who had had antibiotic treatment prior to this study. standard methods were used for the investigations. f ...19958907604
[aerobic and anaerobic bacterial flora in acute abdominal illnesses].366 specinmens taken from patients suffering from acute abdominal illnesses who had undergone operations were examined. bacterial pathogens were isolated from 318 specimens. aerobic bacteria were isolated from 53.8% specimens. anaerobic bacteria were isolated from 12.6% specimens. mixed bacterial flora were isolated from 33.6% specimens. among aerobic bacteria e. coli and streptococcus faecalis were most often isolated. among anaerobic bacteria bacteroides fragilis, peptostreptococcus spp. and p ...19958833932
crystallization and x-ray analysis of a single fab binding domain from protein l of peptostreptococcus magnus.protein l is a multidomain cell wall constituent of certain strains of peptostreptococcus magnus which binds to the variable domain of immunoglobulin kappa-light chains. a single immunoglobulin-binding domain of m(r) = 9000 from this protein has been isolated and crystallized. the crystals are of space group p4(2)2(1)2, with cell dimensions a = b = 66.9 a, c = 68.3 a, and diffract to at least 2.2 a resolution. the asymmetric unit of the crystal contains two molecules of the protein l domain, rel ...19958749861
pneumonia in mechanically ventilated children.the quantitative aerobic and anaerobic microbiology of bronchial aspirates, obtained using protective brush catheters, from 10 children with ventilator-associated pneumonia, is presented. aerobic or faculative organisms only were isolated in 1 child, anaerobic bacteria only in 3, and aerobic mixed with anaerobic bacteria in 6. there were 10 aerobic or faculative and 17 anaerobic isolates. the predominant aerobes were pseudomonas aeruginosa (2 isolates) and klebsiella sp. (2). the predominant ana ...19958685643
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
cloning, expression and purification of ppl-1, a kappa-chain binding protein, based upon protein l from peptostreptococcus magnus.protein l is a multi-domain, cell wall constituent of certain strains of peptostreptococcus magnus, which binds to the variable domain of the l-chains of ig. a gene fragment which codes for a single ig-binding domain of protein l (ppl-1) was cloned into a modified pkk223-3 vector and over-expressed in e. coli jm103. a rapid protein purification protocol is described. in these studies, purified ppl-1 was immobilised on to an agarose gel and tested against an array of igs and ig fragments. it was ...19958767928
a risk-benefit assessment of teicoplanin in the treatment of infections.teicoplanin is a glycopeptide antibiotic whose activity is selectively oriented against gram-positive aerobic and anaerobic bacteria, including staphylococcus aureus, coagulase-negative staphylococci, clostridium difficile, peptostreptococcus spp. and corynebacterium jeikeium; such activity is affected by neither methicillin resistance nor beta-lactamase production. teicoplanin is not significantly absorbed from the gastrointestinal tract; consequently, it has to be administered intravenously (e ...19958785019
techniques for controlling variability in gram staining of obligate anaerobes.identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...19957538512
translation of trpg in bacillus subtilis is regulated by the trp rna-binding attenuation protein (trap).trpg of bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and l-tryptophan. expression of trpg is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. examination of both transcriptional and translational gene fusions to lacz involving trpg and direct measurements of trpg mrna levels and trpg polypeptide accumulation demonstrated that translation of trpg is regulated by trypt ...19957543470
role of the reference laboratory in susceptibility testing of anaerobes and a survey of isolates referred from laboratories in england and wales during 1993-1994.methods for susceptibility testing of anaerobes and their patterns of susceptibility remain debatable. the responsibilities of the public health laboratory service anaerobe reference unit include identification of referred isolates; development of methods; monitoring of the occurrence of anaerobic infections; performance of taxonomic studies; maintenance of a reference collection; and monitoring of the antibiotic susceptibility of clinical isolates. selection of antibiotics for anaerobic infecti ...19957548547
bacteriology of chronic otitis media, chronic sinusitis, and paranasal mucopyocele in japan.a bacteriologic study of cases of chronic otitis media, chronic sinusitis, and paranasal mucopyocele diagnosed at gifu university hospital was performed between 1989 and 1992. bacteria were isolated from all of 13 samples of discharge from the ears of patients with chronic otitis media, all of 14 mucosal biopsy specimens from patients with chronic sinusitis, 8 of 10 aspirate samples from patients with chronic sinusitis, and 9 of 15 aspirates samples from patients with mucopyocele. cultures often ...19957548557
a retrospective review of cases of anaerobic empyema and update of bacteriology.we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ...19957548560
role of bacterial vaginosis in pelvic inflammatory disease.pelvic inflammatory disease (pid) is a frequent infection in sexually active young women and results in adverse sequelae, including tubal-factor infertility and ectopic pregnancy. in the 1970s investigations using culdocentesis demonstrated that anaerobic bacteria played an important role in the etiology of pid. this finding has subsequently been confirmed by studies utilizing laparoscopy and/or endometrial biopsy to obtain specimens directly from the upper genital tract (uterine cavity and fall ...19957548573
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...19957548575
foot infections in diabetic patients: the role of anaerobes.the prevalence of anaerobic bacteria in cultures of specimens from foot infections in diabetic patients is dependent upon the method of obtaining the specimen, the care with which it is transported anaerobically, and the sophistication of the laboratory methods. the rate at which anaerobes are isolated with use of the best methods ranges from 74% to 95% of patients, but it is only 41% to 53% in clinical studies of patients with limb-threatening infection. the mean number of bacterial isolates fr ...19957548576
a phage display system for studying the sequence determinants of protein folding.we have developed a phage display system that provides a means to select variants of the igg binding domain of peptostreptococcal protein l that fold from large combinatorial libraries. the premise underlying the selection scheme is that binding of protein l to igg requires that the protein be properly folded. using a combination of molecular biological and biophysical methods, we show that this assumption is valid. first, the phage selection procedure strongly selects against a point mutation i ...19957549875
the microbiology and antibiotic treatment of peritonsillar abscesses.pus from 53 peritonsillar abscesses was cultured and associations between the microbiological results and clinical data were investigated with the aim of developing a clinical protocol for treatment. a positive culture grew in 85% of quinsies and of these 16% produced aerobes and 84% anaerobes. penicillin-resistant organisms were grown from 32% of patients and all but one of these organisms (haemophilus influenzae) was sensitive to metronidazole. there was no association between clinical present ...19957554331
microbiology of secondary bacterial infection in scabies lesions.aerobic and anaerobic bacteria were grown from specimens obtained from 30 children with secondarily infected scabies lesions. aerobic or facultative bacteria only were present in 14 (47%) patients, anaerobic bacteria only were present in 6 (20%) patients, and a mixed anaerobic-aerobic flora was present in 10 (33%) patients. fifty isolates were recovered (1.7 per specimen); 27 were aerobic or facultative bacteria and 23 were strict anaerobes. the predominant aerobic and facultative bacteria were ...19957559963
in vitro activity of bay y 3118, and nine other antimicrobial agents against anaerobic bacteria.the antibacterial activity of bay y 3118, a new chlorofluoroquinolone, was determined against 257 strains of anaerobic bacteria and compared with the activities of ofloxacin, ciprofloxacin, sparfloxacin, imipenem, cefoxitin, clindamycin, chloramphenicol, metronidazole, and ornidazole. overall, bay y 3118 was the most active agent tested against the bacteroides fragilis group. its activity (mic90, 0.5 mg/l) was 16-fold lower than that of sparfloxacin (mic90, 8 mg/l), and more than 100-fold lower ...19957562012
[in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria].during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ...19957567117
changes in the core tonsillar bacteriology of recurrent tonsillitis: 1977-1993.microbiological studies of the core of tonsils removed from children with recurrent tonsillitis due to group a beta-hemolytic streptococci were conducted during three periods, with 50 patients in each period: 1977-1978 (period 1), 1984-1985 (period 2), and 1992-1993 (period 3). mixed flora were present in all tonsils, with 8.1 organisms per tonsil (3.8 aerobes and 4.3 anaerobes). the predominant isolates in each period were staphylococcus aureus, moraxella catarrhalis, peptostreptococcus species ...19957578726
recombinant proteins l and lg: efficient tools for purification of murine immunoglobulin g fragments.in order to improve antibody purification methods, recombinant proteins l and lg were tested in the purification of murine monoclonal immunoglobulin g (igg) and its fragments. after affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. affinity chromatographic experiments confirme ...19957581856
the role of cefotaxime in the treatment of surgical infections.this study examines the role of cefotaxime in the treatment of both gram-negative and gram-positive surgical infections. a dose of 2 g of cefotaxime will sustain peripheral compartment concentrations of 2.6, 3.9, 1.6, and 0.7 micrograms/ml for 6, 8, 10 and 12 h, respectively. therefore, the proportion of pathogens with a minimal inhibitory concentration (mic) below the peripheral compartment cefotaxime concentrations was assessed as a measure of therapeutic potential. it was observed that bacter ...19957587036
the ga module, a mobile albumin-binding bacterial domain, adopts a three-helix-bundle structure.we present the first study of the secondary structure and global fold of an albumin-binding domain. our data show that the ga module from protein pab, an albumin-binding protein from the anaerobic bacterial species peptostreptococcus magnus, is composed of a left-handed three-helix bundle. the helical regions were identified by sequential and medium range noes, values of nh-c alpha h coupling constants, chemical shift indices, and the presence of slowly exchanging amide protons, as determined by ...19957589548
role of escherichia coli p fimbriae in intestinal colonization in gnotobiotic rats.adherence via p fimbriae is associated with long-term persistence of escherichia coli in the human large intestine, but a causal relationship has not been proven. in the present study, germfree rats were colonized with a mixture of two isogenic e. coli strains, one p fimbriated and the other type 1 fimbriated. both types of fimbriae conferred adherence to rat colonic epithelial cells. with two mutant strains from a pyelonephritogenic isolate of serotype o75:k5:h-, the p-fimbriated strain 824 att ...19957591136
an approach to efficacy screening of mouthrinses: studies on a group of french products (i). staining and antimicrobial properties in vitro.there is a large and increasing number of oral hygiene products available to the general public. as such, it is difficult to demonstrate efficacy for all in long-term home-use studies. the aim of this project was to determine whether screening studies could position the activity and efficacy of a number of antiseptic mouthrinses, available in france, by comparison with an established product. this study reports the experiments in vitro. the products under test were, 4 containing chlorhexidine (e ...19957593703
factors affecting the occurrence of bacteremia associated with tooth extraction.this study examined the factors that affect the occurrence of bacteremia associated with tooth extraction and the kinds of bacteria causing this bacteremia. bacteremia was found in 132 (72.1%) of 183 patients who had one or more teeth extracted for various reasons. bacteremia occurred more frequently when teeth were extracted because of inflammatory dental diseases. the occurrence of bacteremia also increased with the number of teeth extracted and the age of the patients. when the volume of bloo ...19957594761
antibacterial effects of some root end filling materials.in addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. this investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, super eba and a mineral trioxide aggregate on nine facultative bacteria streptococcus fecalis, streptococcus mitis, streptococcus mutans, streptococcus salivarius, lactobacillus species, staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, and esc ...19957595152
mapping of the immunoglobulin light chain-binding site of protein l.protein l is a cell surface protein expressed by some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule binds specifically and with high affinity to immunoglobulins (ig) of a wide range of animal species. the ig-binding activity is mediated through five highly homologous domains, each 72 to 76 amino acid residues long, which interact with framework regions in the variable domain of ig light chains. the interaction does not interfere with the antigen binding capac ...19957608965
clinical features and aerobic and anaerobic microbiological characteristics of cellulitis.to investigate the aerobic and anaerobic microbiological characteristics of cellulitis and correlate them with the infection site.19957611872
microbiologic and clinical study of polytetrafluoroethylene membranes for guided bone regeneration around implants.this study determined the microbiota of the mucosa- and implant-facing parts of expanded polytetrafluoro ethylene augmentation material, and the influence of major periodontopathogens on the healing process associated with guided bone regeneration around dental implants. seventeen patients with nine dehiscence and eight extraction defects were studied. prior to surgery and at membrane removal, microbial morphotypes, total viable counts, and the occurrence of selected microbial species were exami ...19957615319
prosthetic-valve endocarditis caused by peptostreptococcus anaerobius. 19957620041
periodontal microbiota of mobile and non-mobile teeth.the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ...19957623258
[nosocomial infections in present-day traumatological-orthopedic hospitals].nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...19957626997
a numerical taxonomic study of the gram-positive anaerobic cocci.clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...19957629855
[antibacterial activities of cefmenoxime against recent fresh clinical isolates from patients in sinusitis].in order to evaluate antimicrobial activity of cefmenoxime (cmx), minimum inhibitory concentrations (mics) of cmx and control drugs were determined against clinical isolates from patients of sinusitis that were obtained in our laboratory from october of 1993 to march of 1994. the results are summarized as follows; 1. cmx showed strong antimicrobial activities against streptococcus pneumoniae, haemophilus influenzae and moraxella subgenus branhamella catarrhalis that were 3 major aerobic bacteria ...19957637194
liver abscesses as initial presentation of crohn's disease. 19957639260
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...19957641626
bacteremia in conjunction with endodontic therapy.this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...19957641631
microbiology of gastrostomy site wound infections in children.specimens of pus were obtained from gastrostomy site wound infections in 22 children. polymicrobial flora was found in 21 of the 22 wounds. aerobic or facultative bacteria only were isolated in eight (36%) instances and mixed aerobic-anaerobic flora were isolated from the other 14 (64%) wounds. a total of 102 bacterial isolates (57 aerobic and 45 anaerobic) and seven cultures of candida were obtained. the most frequent isolates were escherichia coli (16 isolates). peptostreptococcus spp. (14), e ...19957650731
multiple ligand interactions for bacterial immunoglobulin-binding proteins on human and murine cells of the hematopoetic lineage.a group of bacterial ig-binding surface proteins were studied: protein h and m1 are from streptococcus pyogenes and interact with igg, protein l is expressed by peptostreptococcus magnus and shows affinity for ig light chains, whereas protein lg is a chimeric construction combining the binding properties of protein l with the igg-binding activity of protein g from group c and g streptococci. proteins l and h coupled to sepharose were mitogenic for human peripheral blood lymphocytes (pbl) and mou ...19957660068
endoscopic treatment of acute frontal sinusitis: indications and limitations. 19957675495
[bone and joint infections due to unusual pathogens].between 1989 and 1993, 7 patients--2 men and 5 women from 19 to 70 years of age--with osteomyelitis due to an unusual organism were observed. 3 cases with salmonella typhimurium and one at a time with escherichia coli, peptostreptococcus, propionibacterium acnes and pasteurella multocida. 5 patients were treated operative accompanied with antibiotics and 1 without operation. 1 patient declined the indicated surgical therapy. the outcome were total healing in 3 cases, healing with ankylosis of th ...19957754663
necrotizing fasciitis of the head and neck with intrathoracic extension.we present the case history of a white male patient who initially presented in the emergency department with a peritonsillar abscess and subsequently returned several days later with necrotizing cervical fasciitis with intrathoracic extension. the successful treatment, utilizing surgical debridement and hyperbaric oxygen, will be discussed for this disease, which causes up to 50% or greater mortality.19957769648
[the prospects for using the new macrolide antibiotic azithromycin (sumamed) in the combined treatment of periodontitis].antibacterial activity of sumamed, a macrolide antibiotic, in respect of anaerobic microflora of the oral cavity, such as bacteroides, prevotella, porphyromonas, fusobacterium, peptostreptococcus, str. sanguis and staph. aureus, was assessed. the drug was found to be highly effective in respect of bacteroids (mpc90: 1,4 to 30 mg/mlmg/ml, this permitting the use of sumamed in the treatment of 9 patients with chronic generalized periodontitis in the exacerbation stage. bacteriological and clinical ...19957770869
crystalline surface protein of peptostreptococcus anaerobius.the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ...19957773401
timing of surgery in patients with infected spinal dermal sinuses: report of two cases.in the past, if infection was present at the time a spinal dermal sinus was discovered, the sinus opening and cerebrospinal fluid were sterilized with a course of appropriate antibiotic therapy prior to the surgical intervention. the cases of two children with an infected spinal dermal sinus are reported here. one patient developed acute paraplegia during the initial stage of antibiotic therapy, and the second patient, who was admitted only with signs of fever and irritability, experienced after ...19957773970
periodontal findings in spouses. a clinical, radiographic and microbiological study.clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...19957775676
sinusitis and bronchial asthma in children.sinus disease has been assumed to exist in children with respiratory tract allergies. we consecutively evaluated 375 patients (245 male, 130 female) with childhood onset asthma, between 5 and 15 years of age (mean 7.8 years of age). abnormal waters radiographs were found in 205 patients (54.7%) which included mucosal wall thickening greater than 6 mm (67.3%), complete opacification (22.9%) and air-fluid levels (9.8%). the level of immunoglobulin (ig) e was found to be much higher in non-sinusiti ...19957778440
role of anaerobic bacteria in chronic otitis media and cholesteatoma.otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...19957782173
advanced periodontitis associated with larsen's syndrome: case report.larsen's syndrome is a genetic disorder of connective tissue characterized by multiple large-joint dislocations, abnormalities of hands and feet, and anomalous facial features. although findings involving multiple systems have been added to its clinical and pathological entity, oral pathology besides cleft palate has not been reported. we report here advanced periodontitis with severe gingival inflammation in a 29-year-old larsen's syndrome patient. bacterial culture of subgingival plaque identi ...19957899108
clinical and microbiological features of necrotizing fasciitis.the microbiological and clinical characteristics of 83 patients with necrotizing fasciitis (nf) treated over a period of 17 years are presented. bacterial growth was noted in 81 of 83 (98%) of specimens from patients with nf. aerobic or facultative bacteria only were recovered in 8 (10%) specimens, anaerobic bacteria only were recovered in 18 (22%) specimens, and mixed-aerobic-anaerobic floras were recovered in 55 (68%) specimens. in total, there were 375 isolates, 105 aerobic or facultative bac ...19957494032
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
[a case of gas-producing brain abscess with subdural empyema: timing of the operation for brain abscess].a case of gas-producing brain abscess with subdural abscess was reported. an 18-year-old boy was admitted with a five-day history of vomiting and high grade pyrexia. plain skull roentgenograms demonstrated left frontal multiple gas bubbles. ct scan and mri showed that both brain and subdural abscesses contained gas in the left frontal area. antibiotics and glyceol were intravenously administrated. in serial ct scans, subdural abscess was not recognized, while brain abscess was enhanced in a ring ...19957477705
intracranial complications of sinusitis in childhood.complications of sinusitis in children, such as intracranial abscess formation, are uncommon and are often clinically unremarkable in comparison to similar disease processes in adults. between 1983 and 1991, 443 children were admitted to children's national medical center in washington, dc, for treatment of sinusitis. fourteen of these children presented with intracranial extension of the infection and abscess formation. a retrospective review of these patients revealed that the risk of developi ...19957717619
microbiology of chronic suppurative otitis media in children in surabaya, indonesia.the aerobic and anaerobic microbiology of 38 children from surabaya, indonesia, who suffered from chronic suppurative otitis media (csom) was studied using strict microbiological methodology. a total of 106 isolates (49 anaerobic and 57 aerobic) were recovered. aerobic organisms alone were isolated from 11 (29%), anaerobic bacteria only in 4 (11%) and mixed aerobic and anaerobic flora were present in 23 (60%). the predominant organisms were peptostreptococcus sp., prevotella sp., bacteroides sp. ...19957729990
the aerobic and anaerobic microbiology of pustular acne lesions.specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria in 6 (18%) specimens. a total of 57 isolates, 31 anaerobes (1.0 per specimen) and 26 aerobes (0.8 per specimen) were recovered. the predominant isolates were staphylococcus sp. (19 i ...199516887540
antimicrobial activity of sch 27899, oligosaccharide member of the everninomycin class with a wide gram-positive spectrum.the in vitro antimicrobial activity of sch 27899 (everninomycin), a novel oligosaccharide compound of the everninomycin class, was compared with vancomycin, chloramphenicol, clinafloxacin, teicoplanin and doxycycline against 428 clinical strains of bacteria. everninomycin base exhibited the greatest antimicrobial activity compared to other formulations against all strains tested (mic90: 0.25 microg/ml) followed by clinafloxacin and teicoplanin (mic90: 0.5 microg/ml), vancomycin (mic90: 2 microg/ ...199511866719
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
recombinant proteins l and lg. two new tools for purification of murine antibody fragments.several bacterial cell wall proteins, like proteins a and g, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. in 1988, protein l, a bacterial cell wall protein with ig-binding capacity, was isolated from the anaerobic bacterial species peptostreptococcus magnus. binding data with immunoglobulin fragments suggested that protein l could selectively bind the variable region of human kappa light chains. more recently a recombinan ...19947736533
the antimicrobial activity of essential oils and essential oil components towards oral bacteria.a method for reproducibly determining minimal inhibitory concentrations and minimal bactericidal concentrations of plant extracts towards fastidiously and facultatively anaerobic oral bacteria, predicated upon measurements of optical densities in microtitre plate wells, was devised. the antimicrobial properties of some botanical oils were surveyed; of these, australian tea tree oil, peppermint oil, and sage oil proved to be the most potent essential oils, whereas thymol and eugenol were potent e ...19947478759
[a case of niddm with non-clostridial gas-producing infection in the lower limb--the effects of hyperbaric oxygen therapy].a 36-year-old male was admitted because of swelling and pain in the lower left limb. he had been diagnosed as having non-insulin dependent diabetes mellitus 4 years previously and had been treated unsuccessfully with insulin. since gas formation was radiographically demonstrated in the soft tissue of the left foot, we treated him with antibiotics and hyperbaric oxygen under the diagnosis of gas gangrene. despite this therapy, the gangrenous lesions progressed. amputation of the left foot was nec ...19948151155
[antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections].to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ...19948151907
relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults.to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ...19948157769
protein pab, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling.some strains of the anaerobic human commensal and pathogen peptostreptococcus magnus bind human serum albumin (hsa), whereas other strains of this species express protein l, an immunoglobulin light chain-binding surface protein. a novel hsa-binding protein called protein pab was purified in one step from the culture supernatant of an hsa-binding strain of p. magnus by affinity chromatography on hsa-sepharose. the apparent size of the molecular was 47 kda on sds-polyacrylamide gel electrophoresis ...19948163519
delta 22-beta-muricholic acid in monoassociated rats and conventional rats.bile acids were analyzed in the bile, small and large intestines, and feces of germ-free rats after a single inoculation with one of six intestinal bacteria that had been originally isolated from human feces. bacteroides vulgatus and bifidobacterium longum preferentially deconjugated tauro-beta-muricholic acid and taurocholic acid, respectively. clostridium ramosum, peptostreptococcus productus and lactobacillus gasseri deconjugated both bile acids, but escherichia coli did not deconjugate eithe ...19948177022
antibacterial activity of clavulanate and tazobactam on peptostreptococcus spp. 19948182024
an unclassified eubacterium taxon in acute dento-alveolar abscess.the microflora of pus samples aspirated from 50 acute dento-alveolar abscesses was examined. a total of 143 bacterial strains was isolated, consisting predominantly of prevotella spp., alpha-haemolytic streptococcus spp., peptostreptococcus spp. and eubacterium spp. an unclassified asaccharolytic eubacterium taxon was encountered in 17 (34%) of the abscesses. this taxon was found to have a positive association with fusobacterium spp. and a negative association with alpha-haemolytic streptococcus ...19948107059
electrogenic glutamine uptake by peptostreptococcus anaerobius and generation of a transmembrane potential.peptostreptococcus anaerobius converted glutamine stoichiometrically to ammonia and pyroglutamic acid, and the eadie-hofstee plot of glutamine transport was biphasic. high-affinity, sodium-dependent glutamine transport (affinity constant [kt] of 1.5 microm) could be driven by the chemical gradient of sodium, and more than 20 mm sodium was required for half-maximal velocity. high-affinity glutamine transport was not stimulated or inhibited by a membrane potential (delta psi). low-affinity glutami ...19948113169
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
bacterial colonization and degradation of demineralized dentin matrix in situ.lesion formation in dentin involves demineralization followed by degradation of the exposed organic matrix. proteinases from microorganisms present in the dentin are believed to play an important role in the breakdown of the dentinal collagen. in this study, the microflora colonizing decalcified dentin matrix, placed in situ, was identified. the gelatinolytic activity of the isolated strains was assessed and related to the degradation of the dentin matrix. the predominant species found were stre ...19948124693
bacterial vaginosis and vaginal microorganisms in idiopathic premature labor and association with pregnancy outcome.the vaginal microflora of 49 women in idiopathic preterm labor was compared with that of 38 term controls to determine whether the presence of bacterial vaginosis (bv) and/or specific microorganisms would influence the rate of preterm delivery. demographic factors, pregnancy outcome, and reproductive history were also studied. bv, as defined by the presence of clue cells in a vaginal wet mount and characteristic microbial findings in a stained vaginal smear and vaginal culture, was more common i ...19948126176
determination of 16s ribosomal rna sequences of all members of the genus peptostreptococcus and their phylogenetic position.a comparative analysis of 16s ribosomal rna sequences of all peptostreptococcus species revealed that most members of the genus peptostreptococcus should be divided into many different genera. the relationship between clostridia and peptostreptococci was analysed to find the phylogenetic position of peptostreptococci.19948132148
demonstration of human immunoglobulin g fc-binding activity in oral bacteria.nonimmune binding of immunoglobulins via the fc fragment may reduce opsonization and phagocytosis of bacteria and is thus considered a virulence factor. the aim of this study was to investigate a wide range of oral bacterial strains for the presence of human immunoglobulin g (igg) fc-binding activity. a total of 132 strains representing 40 different gram-positive and gram negative bacterial species were tested for igg fc-binding activity by using a fast and simple dot blot procedure with horsera ...19947496956
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