Publications

inactivation of bacteriophage infecting bacteroides strain gb124 using uv-b radiation.	ultraviolet-b radiation (280-320 nm) has long been associated with the inactivation of microorganisms in the natural environment. determination of the environmental inactivation kinetics of specific indicator organisms [used as tools in the field of microbial source tracking (mst)] is fundamental to their successful deployment, particularly in geographic regions subject to high levels of solar radiation. phage infecting bacteroides fragilis host strain gb124 (b124 phage) have been demonstrated t ...	2014	24329482
the ellis island effect: a novel mobile element in a multi-drug resistant bacteroides fragilis clinical isolate includes a mosaic of resistance genes from gram-positive bacteria.	objectives: bacteroides fragilis, a gram-negative anaerobic bacterium, is alternately a gut commensal or virulent pathogen and is an important reservoir for horizontal gene transfer (hgt) of bacterial resistance and virulence genes in the human gastrointestinal tract. we identified a unique conjugative transposon (ctn) in a multidrug resistant clinical isolate of b. fragilis (bf-hmw615); we named this element ctnhyb because it included a hybrid mosaic of foreign elements. this study reports the ...	2014	25165618
crystallization, preliminary x-ray crystallographic and cryo-electron microscopy analysis of a bifunctional enzyme fucokinase/l-fucose-1-p-guanylyltransferase from bacteroides fragilis.	fucokinase/l-fucose-1-p-guanylyltransferase (fkp) is a bifunctional enzyme which converts l-fucose to fuc-1-p and thence to gdp-l-fucose through a salvage pathway. the molecular weights of full-length fkp (f-fkp) and c-terminally truncated fkp (c-fkp, residues 300-949) are 105.7 and 71.7 kda, respectively. in this study, both recombinant f-fkp and c-fkp were expressed and purified. size-exclusion chromatography experiments and analytical ultracentrifugation results showed that both f-fkp and c-f ...	2014	25195892
efficient utilization of complex n-linked glycans is a selective advantage for bacteroides fragilis in extraintestinal infections.	bacteroides fragilis is the most common anaerobe isolated from clinical infections, and in this report we demonstrate a characteristic of the species that is critical to their success as an opportunistic pathogen. among the bacteroides spp. in the gut, b. fragilis has the unique ability of efficiently harvesting complex n-linked glycans from the glycoproteins common to serum and serous fluid. this activity is mediated by an outer membrane protein complex designated as don. using the abundant ser ...	2014	25139987
bacteroides fragilis subverts mucosal biology: from symbiont to colon carcinogenesis.	the human body comprises fewer host cells than bacterial cells, most of which are obligate anaerobes residing in the gut. the symbiont bacteroides fragilis constitutes a relatively small proportion (up to 1%-2%) of cultured fecal bacteria, but colonizes most humans. there are 2 classes of b. fragilis distinguished by their ability to secrete a zinc-dependent metalloprotease toxin, b. fragilis toxin (bft). strains that do not secrete bft are nontoxigenic b. fragilis (ntbf), and those that do are ...	2014	25105360
digesting the emerging role for the gut microbiome in central nervous system demyelination.	the fields of microbiology, immunology, neurology and nutrition are rapidly converging, as advanced sequencing and genomics-based methodologies have enabled the mapping out of the microbial diversity of humans for the first time. bugs, guts, brains and behavior were once believed to be separate domains of clinical practice and research; however, recent observations in our understanding of the microbiome indicate that the boundaries between domains are becoming permeable. this permeability is mul ...	2014	25070675
antimicrobial susceptibility of clinical isolates of anaerobic bacteria in ontario, 2010-2011.	the local epidemiology of antimicrobial susceptibility patterns in anaerobic bacteria is important in guiding the empiric treatment of infections. however, susceptibility data are very limited on anaerobic organisms, particularly among non-bacteroides organisms. to determine susceptibility profiles of clinically-significant anaerobic bacteria in ontario canada, anaerobic isolates from sterile sites submitted to public health ontario laboratory (phol) for identification and susceptibility testing ...	2014	24923267
distinct cytokine pattern in response to different bacterial pathogens in human brain abscess.	brain abscess is a focal suppurative process. host inflammatory response in gram type and specific bacteria has not been studied in brain abscess. a total of 57 brain abscess patients with monomicrobial infections were studied for th1 (tnf-α, ifn-γ, il1-β), th2 (il-4, il-10) and th17 (il-17, il-23) cytokine response by reverse-transcriptase pcr and elisa. th1 and th17 cytokines were significantly elevated in gram positive (staphylococcus aureus and streptococcus intermedius) and th2 cytokine (il ...	2014	24910026
identification of genes required for the survival of b. fragilis using massive parallel sequencing of a saturated transposon mutant library.	bacteroides fragilis is a gram-negative anaerobe that is normally a human gut commensal; it comprises a small percentage of the gut bacteroides but is the most frequently isolated bacteroides from human infections. identification of the essential genes necessary for the survival of b. fragilis provides novel information which can be exploited for the treatment of bacterial infections.	2014	24899126
the reca operon: a novel stress response gene cluster in bacteroides fragilis.	bacteroides fragilis, an opportunistic pathogen of humans, is a leading cause of bacteraemias and anaerobic abscesses which are often treated with metronidazole, a drug which damages dna. this study investigated the responses of the b. fragilis reca three gene operon to the stress experienced during metronidazole treatment and exposure to reactive oxygen species simulating those generated by the host immune system during infection. a transcriptionally regulated response was observed using quanti ...	2014	24703997
analysis of the arabinose-5-phosphate isomerase of bacteroides fragilis provides insight into regulation of single-domain arabinose phosphate isomerases.	arabinose-5-phosphate isomerases (apis) catalyze the interconversion of d-ribulose-5-phosphate and d-arabinose-5-phosphate, the first step in the biosynthesis of 3-deoxy-d-manno-octulosonic acid (kdo), an essential component of the lipopolysaccharide in gram-negative bacteria. classical apis, such as escherichia coli kdsd, contain a sugar isomerase domain and a tandem cystathionine beta-synthase domain. despite substantial effort, little is known about structure-function relationships in these a ...	2014	24891442
a pilot study on the effect of lactobacillus casei zhang on intestinal microbiota parameters in chinese subjects of different age.	ageing of the population is an imminent global problem. lactobacillus casei zhang (lcz) was isolated from inner mongolian fermented milk, koumiss. lcz possesses numerous probiotic properties in in vitro tests and in animal models. however, it has never been tested in any human trial. in the current study, the impact of oral consumption of lcz on different age groups was tested. chinese subjects, including 10 young, 7 middle-aged and 7 elderly volunteers (with mean age of 24.3, 47.6 and 64.7, res ...	2014	24854958
antibiotic resistance capability of cultured human colonic microbiota growing in a chemostat model.	to evaluate the potential antibiotic resistance capability of cultured human colonic microbiota as a whole system when residual antibiotics enter the human intestine, the combination of viable cell counting and denaturing gradient gel electrophoresis (dgge) method was used to study effects of the enrofloxacin (enr) residue on the microbial diversity, antibiotic resistance, and anti-colonization capability in a human chemostat model. the results indicated that the enr enhanced the microbial antib ...	2014	24715638
stat3 activation in murine colitis induced by enterotoxigenic bacteroides fragilis.	enterotoxigenic bacteroides fragilis (etbf), a molecular subclass of the common human commensal, b. fragilis, has been associated with inflammatory bowel disease. etbf colitis is characterized by the activation of stat3 and a th17 immune response in the colonic mucosa. this study was designed to investigate the time course and cellular distribution of stat3 activation in etbf-colonized mice.	2014	24704822
prevalence of in vitro susceptibility to carbapenems among clinical isolates of the bacteroides fragilis group from a colombian tertiary hospital.	clinical isolates of the bacteroides fragilis group (n=29) were collected from patients attending a tertiary care hospital in medellin, colombia, during 2012 and were tested for susceptibility to four carbapenems using the clinical and laboratory standards institute (clsi) etest method. overall rates of percent susceptibility were as follows: meropenem, 93.1%; imipenem, 100%; ertapenem, 96.6%; and doripenem, 93.1%. this is the first study to show rates of resistance of bacteroides spp. to carbap ...	2014	27873638
detection of carbapenemase activities of bacteroides fragilis strains with matrix-assisted laser desorption ionization--time of flight mass spectrometry (maldi-tof ms).	today resistance against carbapenems is considered an emerging problem in bacteroides fragilis. carbapenemase activities produced by aerobic bacteria have been detected by looking at hydrolysis of carbapenems with maldi-tof ms, but this technique was never used for anaerobic bacteria. we have developed a protocol for detection and verification of carbapenemase production in b. fragilis within 2.5 h. twenty-eight strains of b. fragilis were tested. of the sixteen cfia-positive strains all showed ...	2014	24480431
epidemiology and spectrum of positive bacteriological culture in intrauterine fluid collected from women with postmenopausal bleeding.	this study aims to examine the prevalence and bacteriological findings of different types of intrauterine fluid collection in women presenting with postmenopausal bleeding and the risk factors for predicting positive microbiological culture, mixed growth, and anaerobic growth.	2014	24473531
microbes in the neonatal intensive care unit resemble those found in the gut of premature infants.	the source inoculum of gastrointestinal tract (git) microbes is largely influenced by delivery mode in full-term infants, but these influences may be decoupled in very low birth weight (vlbw, <1,500 g) neonates via conventional broad-spectrum antibiotic treatment. we hypothesize the built environment (be), specifically room surfaces frequently touched by humans, is a predominant source of colonizing microbes in the gut of premature vlbw infants. here, we present the first matched fecal-be time s ...	2014	24468033
a study on nim expression in bacteroides fragilis.	members of the genus bacteroides, mainly bacteroides fragilis, can cause severe disease in man, especially after intestinal perforation in the course of abdominal surgery. treatment is based on a small number of antibiotics, including metronidazole, which has proved to be highly reliable throughout the last 40 to 50 years. nevertheless, metronidazole resistance does occur in bacteroides and has been mainly attributed to nim proteins, a class of proteins with a suggested nitroreductase function. ...	2014	24448511
bacterial profile and patterns of antimicrobial drug resistance in intra-abdominal infections: current experience in a teaching hospital.	bacterial isolates from intra-abdominal infections, in particular, peritonitis and their unpredictable antimicrobial resistance patterns, continue to be a matter of concern not only globally but regionally too.	2014	24441227
sphingolipids from a symbiotic microbe regulate homeostasis of host intestinal natural killer t cells.	coevolution of beneficial microorganisms with the mammalian intestine fundamentally shapes mammalian physiology. here, we report that the intestinal microbe bacteroides fragilis modifies the homeostasis of host invariant natural killer t (inkt) cells by supplementing the host's endogenous lipid antigen milieu with unique inhibitory sphingolipids. the process occurs early in life and effectively impedes inkt cell proliferation during neonatal development. consequently, total colonic inkt cell num ...	2014	24439373
evaluation of the in vitro activity of levornidazole, its metabolites and comparators against clinical anaerobic bacteria.	this study evaluated the in vitro anti-anaerobic activity and spectrum of levornidazole, its metabolites and comparators against 375 clinical isolates of anaerobic bacteria, including gram-negative bacilli (181 strains), gram-negative cocci (11 strains), gram-positive bacilli (139 strains) and gram-positive cocci (44 strains), covering 34 species. minimum inhibitory concentrations (mics) of levornidazole, its five metabolites and three comparators against these anaerobic isolates were determined ...	2014	25301712
understanding how commensal obligate anaerobic bacteria regulate immune functions in the large intestine.	the human gastrointestinal tract is colonised by trillions of commensal bacteria, most of which are obligate anaerobes residing in the large intestine. appropriate bacterial colonisation is generally known to be critical for human health. in particular, the development and function of the immune system depends on microbial colonisation, and a regulated cross-talk between commensal bacteria, intestinal epithelial cells and immune cells is required to maintain mucosal immune homeostasis. this home ...	2014	25545102
prevalence of extended spectrum beta-lactamase producing anaerobic bacteria in chronic periodontitis.	due to the rampant use of antibiotics bacteria are acquiring resistance to penicillin group of drugs, which results in prescription failure in clinical practice. beta-lactamase producing organisms are not only more virulent they also cause surrounding bacteria to become resistant. hence, this study was undertaken to know the prevalence of extended spectrum beta-lactamase (esbl) producing anaerobic bacteria in chronic periodontitis.	2014	25425816
elucidating structural features of an entirely carbohydrate cancer vaccine construct employing circular dichroism and fluorescent labeling.	the zwitterionic polysaccharide ps a1 from anaerobe bacteroides fragilis atcc 25285/nctc 9343 is known to elicit a t-cell-dependent, major histocompatibility complex class ii (mhcii) immune response through a correspondingly similar protein-antigen-based mechanism/pathway. the biological activity of ps a1 is known to arise from alternating charged motifs on adjacent monosaccharides comprising a tetrameric repeating oligomeric unit creating an alpha-helical secondary structure. however, we have l ...	2014	25383162
an antimicrobial protein of the gut symbiont bacteroides fragilis with a macpf domain of host immune proteins.	bacteroidales are the most abundant gram-negative bacteria of the human intestinal microbiota comprising more than half of the bacteria in many individuals. some of the factors that these bacteria use to establish and maintain themselves in this ecosystem are beginning to be identified. however, ecological competition, especially interference competition where one organism directly harms another, is largely unexplored. to begin to understand the relevance of this ecological principle as it appli ...	2014	25339613
comparison of the transport of bacteroides fragilis and escherichia coli within saturated sand packs.	pathogens in groundwater accounted for ∼50% of waterborne disease outbreaks in the united states between 1971 and 2006. the fast and reliable detection of groundwater microbial contamination and the identification of the contamination sources are of critical importance to the protection of public health. recent studies suggested that fecal anaerobe bacteriodes spp. could be employed as an effective tool for surface water microbial source tracking (mst). the usefulness of bacteroides spp. for gro ...	2014	25448721
mobile elements and viral integrations prompt considerations for bacterial dna integration as a novel carcinogen.	insertional mutagenesis has been repeatedly demonstrated in cancer genomes and has a role in oncogenesis. mobile genetic elements can induce cancer development by random insertion into cancer related genes or by inducing translocations. l1s are typically implicated in cancers of an epithelial cell origin, while alu elements have been implicated in leukemia as well as epithelial cell cancers. likewise, viral infections have a significant role in cancer development predominantly through integratio ...	2014	24956175
long-term monitoring of the human intestinal microbiota from the 2nd week to 13 years of age.	microbial contact begins prior to birth and continues rapidly thereafter. few long term follow-up studies have been reported and we therefore characterized the development of intestinal microbiota of ten subjects from the 2nd week of life to 13 years of age. pcr-denaturing gradient gel electrophoresis combined with several bacterial group-specific primer sets demonstrated the colonization steps of defined bacterial groups in the microbiota. bifidobacterium species were seen throughout the test p ...	2014	24933584
cephalosporinases associated with outer membrane vesicles released by bacteroides spp. protect gut pathogens and commensals against β-lactam antibiotics.	to identify β-lactamase genes in gut commensal bacteroides species and to assess the impact of these enzymes, when carried by outer membrane vesicles (omvs), in protecting enteric pathogens and commensals.	2014	25433011
antimicrobial activity of traditional medicinal plants from ankober district, north shewa zone, amhara region, ethiopia.	traditional medicinal plants have long been used in ethiopia to treat human and livestock ailments. despite a well-documented rich tradition of medicinal plant use in the country, their direct antimicrobial effects are still poorly known.	2014	24392738
structural analysis of arabinose-5-phosphate isomerase from bacteroides fragilis and functional implications.	the crystal structure of arabinose-5-phosphate isomerase (api) from bacteroides fragilis (bfapi) was determined at 1.7 å resolution and was found to be a tetramer of a single-domain sugar isomerase (sis) with an endogenous ligand, cmp-kdo (cytidine 5'-monophosphate-3-deoxy-d-manno-oct-2-ulosonate), bound at the active site. api catalyzes the reversible isomerization of d-ribulose 5-phosphate to d-arabinose 5-phosphate in the first step of the kdo biosynthetic pathway. interestingly, the bound cm ...	2014	25286848
distinct patterns of igg and iga against food and microbial antigens in serum and feces of patients with inflammatory bowel diseases.	inflammatory bowel disease (ibd) is associated with a defective intestinal barrier and enhanced adaptive immune responses against commensal microbiota. immune responses against food antigens in ibd patients remain poorly defined.	2014	25215528
functional identification of a galactosyltransferase critical to bacteroides fragilis capsular polysaccharide a biosynthesis.	capsular polysaccharide a (cpsa), a polymer of a four-sugar repeating unit that coats the surface of the mammalian symbiont bacteroides fragilis, has therapeutic potential in animal models of multiple sclerosis and other autoinflammatory diseases. genetic studies have demonstrated that cpsa biosynthesis is dependent primarily on a single gene cluster within the b. fragilis genome. however, the precise functions of the individual glycosyltransferases encoded by this cluster have not been identifi ...	2014	24997288
preferential packing of acidic glycosidases and proteases into bacteroides outer membrane vesicles.	outer membrane vesicles (omv) are spherical membranous structures released from the outer membrane (om) of gram-negative bacteria. omv have been proposed to play several different roles during both pathogenesis and symbiosis. despite the fact that omv were described several decades ago, their biogenesis is a poorly characterized process. whether omv are produced by an active mechanism or by passive disintegration of the om is a still matter of controversy. bacteroides fragilis and bacteroides th ...	2014	24618254
[basal cell nevus syndrome].	a 90-year-old female presented with multiple nevoid basal cell epitheliomas, skeletal defects and jaw cysts. the syndrome is briefly revisited.	2014	4018338
blood viscosity and peripheral circulation in the newborn infant.		2014	4790388
penicillin and syphilis.		2014	4121853
[morphology and medicine].		2014	4028094
arterial hypertension--northwest florida public health survey. ii. diagnosis and treatment.		2014	4214655
studies on the properties of chemically modified actin. i. photooxidation, succinylation, nitration.		2014	4234480
[prevention of drepanocytic painful crises with hydergine].		2014	4219596
[retinal correspondence observed by infrared fundus haploscope. the 15th report: relationship between binocular depth perception and convergence eye movement].		2014	3993470
the assessment of risk factors in 462 patients with acute renal failure.	risk factors associated with the mortality of patients with acute renal failure (arf) were investigated. this was accomplished by a review of 462 patients with arf and the utilization of a logistic regression analysis to develop a model that can be used to predict the mortality odds for an arf patient. the significant risk factors were age, oliguria, pulmonary and cardiovascular complications, jaundice, and hypercatabolism. based on these factors, our model was able to account for 77% of the mor ...	2014	3970021
[on the problem of the rational allocation and effective use of abailable bed space in district hospitals (based on data form karagandinsk region)].		2014	4234930
boston women's health book collective: women empowering women.		2014	3984355
[clinical-chemical tests to judge the security of the berotec pocketinhaler application (author's transl)].		2014	4817601
do sex hormones affect colorectal cancer?		2014	3926085
computed axial tomography of the heart.	ct scanning provides useful cardiac imaging but has not become a routine clinical tool for heart disease due to long exposure times (2-5 seconds) and the limitation of single slice acquisition. a revolutionary high speed (cine) ct electron beam scanner was designed at ucsf, with multilevel millisecond scanning speed at rates of 17 scans per second. table tilt and swivel permits direct imaging in various planes including the half axis view. images can be analysed as closed loop movies, and quanti ...	2014	3916432
[pulmonary biopsy in the diagnosis of tuberculosis].		2014	3959655
[hematoma of the psoas. comparative diagnostic contributions of ultrasonography and x-ray computed tomography].	a 11 cases study of hematoma of the iliacus muscle underlines better results obtained by c.t. scanner compared to those of ultrasonography examination in term of definite diagnoses. comparison of density values and repeated examination were useful to assert diagnosis and to appreciate the course of the illness. only c.t. scanner was able to show which portion of the muscle was concerned. authors correlated the c.t. scanner results to the nature of the entrapment neuropathy clinically found: an i ...	2014	3910813
a histological and immunoenzymatic study on the histogenesis of "giant cell tumor of bones".	we carried out a prospective histologic and immunoenzymatic study, using lysozyme and ai-antichymotrypsin, of 15 well documented cases of giant cell tumors of bones. the histologic appearance of the majority of the tumors was characterized by great pleomorphism. the predominant histologic patterns could be classified as either fibroblastic or histiocytic. mitoses were seen exclusively on stromal mononuclear cells. all tumors showed positive marking with both lysozyme and ai-antichymotrypsin. the ...	2014	3898044
[therapy of bladder tumors. after care and control].		2014	4337780
nonsteroidal antiinflammatory drugs in the nephrotic syndrome.		2014	3909465
methodological problems of body image research in anorexia nervosa patients.	the significance of the disturbed body image as a psychopathological phenomenon in anorexia nervosa patients is quite evident to clinicians. it appears to be difficult, however, to assess this disturbance in a more objective way. the studies on body image perception discussed here make use of objective psychometric methods of measurement: image marking procedure, visual size estimation apparatus, distorting photograph technique, and video distortion. the following methodological problems are dis ...	2014	3962691
episodic secretion of melatonin in pre- and postpubertal girls and boys.	the concentration of melatonin was determined in serum samples obtained at 15-min intervals during a 4-h period from seven normal girls, 9.5-16.5 yr of age, and eight normal boys, 9.0-16.8 yr of age. all samples were obtained between 0800 and 1300 h. an episodic pattern of melatonin secretion was found in all subjects. there was no significant (p greater than 0.1) difference between girls and boys as to number of episodes [3.1 +/- 0.4 (+/- sd) vs. 3.4 +/- 0.5 per 4 h), absolute melatonin increas ...	2014	3972972
nutritional assessment in patients with chronic gastrointestinal symptoms: comparison of functional and organic disorders.	we assessed the nutritional status of 119 patients with chronic gastrointestinal symptoms due to organic disorders (inflammatory bowel disease, ibd; peptic ulcer, pu; malabsorption syndrome, m; and malignant gastrointestinal tumours, t), by standard anthropometry and marker proteins (albumin; retinol-binding protein, rbp; and thyroxine-binding prealbumin, tbpa). we also studied 31 patients with irritable bowel syndrome (ibs) and 75 age-matched healthy controls (c). compared with healthy controls ...	2014	3926730
[preservation of a fractured tooth].		2014	4533325
[preservation of a fractured tooth].		2014	4533325
alveolar cell carcinoma: does it exist?		2014	4335237
alveolar cell carcinoma: does it exist?		2014	4335237
study of an intravenous fat tolerance test with intralipid. i. application of a micronephelometer.		2014	4425422
gm2 type 2--gangliosidosis (sandhoff's disease)--ocular and pathological manifestations.		2014	4276769
antisocial behaviour in children.		2014	4487629
antisocial behaviour in children.		2014	4487629
[cardiac insufficiency in recent myocardial infarct].		2014	4280621
the pattern of the pathology of ovarian tumours in pregnancy in the singapore-malaysia region.		2014	4258576
[investigations on the accuracy of motor-reflective method according to bojanov for establishing centric occlusion].		2014	4513835
chemotherapy in dental practice. polypeptide antibiotics. 3. polymyxin.		2014	4301675
chemotherapy in dental practice. polypeptide antibiotics. 3. polymyxin.		2014	4301675
endometrial responses to transdermal estradiol in postmenopausal women.	in prospective studies, we have determined the endometrial histologic characteristics and patterns of vaginal bleeding in 12 perimenopausal or postmenopausal women during administration of transdermal estradiol, 0.05 mg daily, given either alone or in combination with a progestogen. in the first study, we administered transdermal estradiol in cyclical fashion for 3 months. outpatient curettage at pretreatment produced no endometrial sample or tissue too scant for assessment from 10 of the 12 pat ...	2014	4025456
an intestinal commensal symbiosis factor controls neuroinflammation via tlr2-mediated cd39 signalling.	the mammalian immune system constitutively senses vast quantities of commensal bacteria and their products through pattern recognition receptors, yet excessive immune reactivity is prevented under homeostasis. the intestinal microbiome can influence host susceptibility to extra-intestinal autoimmune disorders. here we report that polysaccharide a (psa), a symbiosis factor for the human intestinal commensal bacteroides fragilis, protects against central nervous system demyelination and inflammati ...	2014	25043484
plasmacytoid dendritic cells mediate anti-inflammatory responses to a gut commensal molecule via both innate and adaptive mechanisms.	polysaccharide a (psa), the archetypical immunomodulatory molecule of the gut commensal bacteroides fragilis, induces regulatory t cells to secrete the anti-inflammatory cytokine interleukin-10 (il-10). the cellular mediators of psa's immunomodulatory properties are incompletely understood. in a mouse model of colitis, we find that psa requires both innate and adaptive immune mechanisms to generate protection. plasmacytoid dcs (pdcs) exposed to psa do not produce proinflammatory cytokines, but i ...	2014	24721570
distinct polymicrobial populations in a chronic foot ulcer with implications for diagnostics and anti-infective therapy.	polymicrobial infections caused by combinations of different bacteria are being detected with an increasing frequency. the evidence of such complex infections is being revealed through the use of novel molecular and culture-independent methods. considerable progress has been made in the last decade regarding the diagnostic application of such molecular techniques. in particular, 16s rdna-based sequencing and even metagenomic analyses have been successfully used to study the microbial diversity i ...	2014	24679105
diaryl-substituted azolylthioacetamides: inhibitor discovery of new delhi metallo-β-lactamase-1 (ndm-1).	the emergence and spread of antibiotic-resistant pathogens is a global public health problem. metallo-β-lactamases (mβls) such as new delhi mβl-1 (ndm-1) are principle contributors to the emergence of resistance because of their ability to hydrolyze almost all known β-lactam antibiotics including penicillins, cephalosporins, and carbapenems. a clinical inhibitor of mbls has not yet been found. in this study we developed eighteen new diaryl-substituted azolylthioacetamides and found all of them t ...	2014	25048031
structural and functional diversity of metalloproteinases encoded by the bacteroides fragilis pathogenicity island.	bacteroides fragilis causes the majority of anaerobic infections in humans. the presence of a pathogenicity island in the genome discriminates pathogenic and commensal b. fragilis strains. the island encodes metalloproteinase ii (mpii), a potential virulence protein, and one of three homologous fragilysin isozymes (fra; also termed b. fragilis toxin or bft). here, we report biochemical data on the structural-functional characteristics of the b. fragilis pathogenicity island proteases by reportin ...	2014	24698179
gm2 type 2--gangliosidosis (sandhoff's disease)--ocular and pathological manifestations.		2014	4276769
[problem of etopathogenesis and treatment of rosaceiform dermatitis].		2014	4272447
osseous metastasis as the only clinical manifestation of primary cancer of the liver.		2014	4290242
crossed segmental inhibition in muscles.		2014	4239093
[action of k+, na+, ca2+ and ba2+ ions on the spontaneous rhythm of the isolated ventricle of ammocoete lampetra planeri].		2014	4258424
[molecular radiobiology. the role of chemical species derived from water].		2014	4224491
[how can health information in the public health service be worked out successfully?].		2014	4254638
[data on high resolution radioautography of 35s-sulfate in the oviduct of pleurodeles waltlii michah. (amphibia, caudata, salamandriae)].		2014	4277160
chemical synthesis of pyrophosphodiesters of carbohydrates and isoprenoid alcohols. lipid intermediates of bacterial cell wall and antigenic polysaccharide biosynthesis.		2014	4339875
chemical synthesis of pyrophosphodiesters of carbohydrates and isoprenoid alcohols. lipid intermediates of bacterial cell wall and antigenic polysaccharide biosynthesis.		2014	4339875
[method of impregnation of sections glued onto glass].		2014	4188036
[the treatment of retinal venous thrombosis with an association of sp54 (fibrase) and diisopropylammonium dichloroethanoate (diedi)].		2014	4190663
mechanisms of chromation hematoxylin stains.		2014	4189134
digital computer classifications of eeg sleep stages.		2014	4182894
proteinuria and nephrotic syndrome associated with chronic rejection of kidney transplants.		2014	4167288
now for the difficult ones.		2014	4167283
[evaluation and appraisal of occupational dermatoses].		2014	4267497
[evaluation and appraisal of occupational dermatoses].		2014	4267497
cutaneous side effects of oral contraceptives.		2014	4244175
the challenge of research in oral pediatrics.		2014	4221363
microtubules and neurofilaments: possible implications in axoplasmic transport.		2014	4108331
[role and task distribution within the family].		2014	4096588
dental education: the concern of all dentistry.		2014	4181143
[blalock-hanlon procedure for mitral atresia: a case report].		2014	4074061