Publications

enrichment medium for isolation of campylobacter jejuni-campylobacter coli.	a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ...	1985	4026287
campylobacter colitis: histological immunohistochemical and ultrastructural findings.	the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ...	1985	4029720
occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals.	serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ...	1985	4044793
comparison of methods for isolating campylobacter jejuni from raw milk.	the method of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) was compared with that of lovett et al. (appl. environ. microbiol. 46:459-462, 1983) for the ability to recover campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. the method of lovett et al. gave significantly greater recovery proportions.	1985	4051491
reversible expression of flagella in campylobacter jejuni.	campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype.	1985	4066041
[clinical studies on the effectiveness of sm-4300, a new non-modified gammaglobulin preparation suitable for intravenous use, in refractory infections].	eighteen cases of various infections, mostly having severe underlying diseases and showing no or insufficient responses to antibacterial chemotherapy, were treated with additional intravenous drip infusion of sm-4300 (2.5 or 5 g, once a day, for 1-3 days). eight of the cases were excluded from the assessment of the effectiveness, as they died too early, or as their fever was supposed to have been "tumor fever" retrospectively. sm-4300 was found to be effective in 2 out of 5 septicemia cases, in ...	1985	4079018
[bacteremia caused by campylobacter fetus ssp. fetus].		1985	4079571
biotyping schemes for campylobacter jejuni.		1985	4031047
investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines.	the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ...	1985	4067286
comparison of gauze swabs and membrane filters for isolation of campylobacter spp. from surface water.	the epidemiology of campylobacter jejuni indicates that waterborne transmission is important; the organism has been isolated from seawater, fresh water, and estuarine sites. membrane filtration, with and without use of an enrichment broth, has been the most common method for isolating c. jejuni from water. we evaluated two methods for isolating c. jejuni from water: membrane filtration and gauze filtration. the membrane filters evaluated included 0.22- and 0.45-micron-pore millipore filters (mil ...	1985	4073894
inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media.	the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ...	1985	4077962
western blot analysis of the human antibody response to campylobacter jejuni cellular antigens during gastrointestinal infection.	western blot analysis was used to identify antigenic components of campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. acute- and convalescent-phase sera from eight patients were analyzed for antibody activity against their homologous infecting strains and heterologous clinical isolates. whole-cell and sarkosyl-insoluble membrane components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tra ...	1985	2578479
antigenic heterogeneity of lipopolysaccharides from campylobacter jejuni and campylobacter fetus.	the lipopolysaccharide (lps) structure of campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase k-treated whole cell lysates. polyacrylamide gel electrophoresis lps profiles of c. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant c. fetus strains have smooth-type lps, and serum-sensitive c. fetus strains have rough-type lps. we electroblotted the proteinase k-treated whole cell ...	1985	2580793
interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies.	lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ...	1985	2410445
antigenic analysis of campylobacter flagellar protein and other proteins.	outer membrane proteins of campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to c. jejuni. each campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of dna relat ...	1985	2578478
[isolation of campylobacter fetus subsp. jejuni from feces and transport media during a diarrhea epidemic].		1985	3155666
[purulent pericarditis caused by campylobacter fetus ssp fetus].		1985	3157173
in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam.	the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam.	1985	2994557
participation of cytochromes in some oxidation-reduction systems in campylobacter fetus.	campylobacter species are rich in c-type cytochromes, including forms which bind carbon monoxide. the role of the various forms of cytochromes in campylobacter fetus has been examined in cell-free preparations by using physiological electron donor and acceptor systems. under anaerobic conditions, nadph reduced essentially all of the cytochrome c in crude cell extracts, whereas the reduction level with succinate was 50 to 60%. the carbon monoxide spectrum with nadph was predominated by the cytoch ...	1985	2995315
[meningitis caused by campylobacter fetus fetus. a case report].		1985	2931701
a two-year longitudinal study on the etiology of acute diarrhea in young children in northern italy.	during a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. patients positive for one or more pathogens were 359 (64.0%). infection with one pathogen was found in 266 (47.4%) patients, whereas multiple infection was detected in 93 (16.6%) patients. enteropathogens associated with disease were rotaviruses: 150 (26.7%), adenoviruses: 99 (17,6%), non-polio enteroviruses: 48 (8.5%), coronaviruses: 10 (1.8%), parvoviruses: ...	1985	2983179
dna relatedness and biochemical features of campylobacter spp. isolated in central and south australia.	investigations of the etiology of diarrhea in patients in south australia and the northern territory showed that campylobacter spp. other than campylobacter jejuni and c. coli were common in children. campylobacters which were hippurate positive, nitrate negative, and susceptible to cephalothin and polymyxins were shown to be closely related to c. jejuni by dna studies. thermotolerant catalase-negative campylobacters were also isolated. these were h2s negative and biochemically resembled the cat ...	1985	2991331
basis for serological heterogeneity of thermostable antigens of campylobacter jejuni.	lipopolysaccharides (lps) were extracted from eight strains of campylobacter jejuni and purified by enzyme treatment to remove traces of rna, dna, and protein. this material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype c. jejuni. the results confirmed that the thermostable antigen typing scheme is based on lps (o) antigens. the lps after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to ...	1985	2412962
typing of campylobacter fetus fetus isolated from sheep abortions in n.z.		1985	16031180
typing of campylobacter fetus fetus isolated from sheep abortions in new zealand.		1985	16031151
serology of campylobacter fetus fetus strains from four outbreaks of ovine abortion.	as a result of a ministry of agriculture & fisheries survey on ovine abortions, 76 isolates of campylobacterfetus fetus were obtained. these isolates were from four farms in the southern hawkes bay, with an abortion incidence of 2.8% to 9.1%. antisera to eight different strains of c. fetus fetus were made in rabbits. strains were then examined using whole cell tube agglutination tests and sensitised staphylococcal protein a slide agglutination tests. heat labile antigens were examined by absorbi ...	1984	16031007
three-year prospective study of intestinal pathogens in madrid, spain.	during the period july 1980 through june 1983, in a general hospital in madrid, the following organisms were detected from 6,970 patients with gastroenteritis: 710 salmonella spp. 506 campylobacter jejuni, 379 shigella spp., 12 yersinia enterocolitica, 1,466 rotavirus, 134 giardia lamblia, and 4 entamoeba histolytica. chloramphenicol showed good activity against most tested strains of salmonella spp., shigella spp., and c. jejuni. the incidence of salmonella spp. and shigella spp. was very marke ...	1984	6092418
[campylobacter fetus ssp fetus thrombophlebitis].		1984	6229730
investigation of a campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis.	fifty campylobacter jejuni isolates, including 29 from humans associated with an outbreak of enteritis, 20 from cattle, and 1 from a milk source, were serotyped on the basis of extractable thermostable antigens and examined by bacterial chromosomal restriction endonuclease digest analysis. serotyping showed specific differences between the human isolates and the milk isolates, but each of these generally, although not consistently, reacted with 4 of the 42 c. jejuni typing antisera. restriction ...	1984	6325487
parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the central african republic.	a total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from march 1981 through february 1982 in the central african republic. one or more pathogens were identified from 49.4% of the patients. rotavirus was the most frequently identified pathogen among children less than 18 months old. enteropathogenic escherichia coli was the second most frequently isolated pathogen (12.1%) in children less than 2 years of age. campylobact ...	1984	6330161
restriction endonuclease analysis of campylobacter strains with particular reference to campylobacter fetus ss. fetus.	forty-three strains of campylobacter fetus ss. fetus isolated from sheep abortions in new zealand, and reference strains of c. fetus ss. fetus (four), c. fetus ss. venerealis (two), c. jejuni (one) and c. coli (one) were examined by restriction endonuclease analysis with the enzymes bste ii and xho i. dna fragment patterns of c. fetus, c. jejuni and c. coli differed strikingly from each other, but there were many similarities in the patterns obtained for all strains of c. fetus ss. fetus (47) an ...	1984	6086928
human antibody response to outer membrane proteins of campylobacter jejuni during infection.	two techniques were used to isolate outer membrane proteins from campylobacter jejuni, edta-lysozyme extraction and sodium-n-lauroylsarcosinate (sarkosyl) solubilization. the protein profiles of the two preparations were similar, with a few additional bands in the edta-lysozyme preparations. the major outer membrane protein was 43,000 (43k) daltons, and there were 8 to 10 minor bands ranging from 92k to 14k daltons. there was no difference in the protein profile of a strain causing an infection ...	1984	6198286
[bacteriocinogenicity of brucellae isolated in foci in the caucasus and their evaluation from taxonomic viewpoints].	the aim of the study was to elucidate the possibility of using bacteriocinogenicity of brucella as taxonomic feature, to determine their phylogenetic relation to other microorganisms by their bacteriocinogenic properties and to investigate the physicochemical properties of brucellacin and conditions for its stable detection. the brucella cultures were isolated in the caucasus. investigation of their capacity for production of bacteriocin according to the procedure described by m.a. konstantinova ...	1984	6230043
procedure for increased recovery of campylobacter jejuni from inoculated unpasteurized milk.	different treatments were applied to campylobacter jejuni-inoculated unpasteurized milk to identify means of enhancing the survival of the organism in refrigerated (4 degrees c) samples. the greatest survival occurred in milk supplemented with 0.01% sodium bisulfite and held under an atmosphere of 100% nitrogen (bisulfite-nitrogen), in most instances allowing isolation of c. jejuni from highly contaminated milk 15 or more days longer than from unsupplemented milk held in air (21% oxygen). althou ...	1984	6370137
[an optimal blood culture technic].	for blood cultures in septicemic patients it is advisable to use two broth culture bottles. one bottle should be incubated with air, the other without (for anaerobes). supplemented peptone broth ii (becton-dickinson) and bhi roche were superior for culturing aerobic and anaerob bacteria in comparison to other blood culture media. they are commercially available, and with these media it is not necessary to inoculate two bottles with blood from one patient. supplemented peptone broth ii is also su ...	1984	6374431
campylobacter fetus ssp fetus cholecystitis and relapsing bacteremia in a patient with acquired immunodeficiency syndrome.	acute gangrenous cholecystitis and relapsing bacteremia caused by campylobacter fetus ssp fetus occurred in a patient with acquired immunodeficiency syndrome. biliary tract colonization probably accounted in part for this unusual phenomenon. whether the patient's deficiency of cell-mediated immunity contributed to his disease is not known.	1984	6377511
immune-mediated migration of neutrophils into the uterine lumen of guinea pigs.	metritis was elicited by intrauterine infusion of tuberculin or killed campylobacter fetus ssp. venerealis into vaccinated guinea pigs and lipopolysaccharide or immune complexes into normal animals. the local inflammatory response to intrauterine infusion of antigens, lipopolysaccharide, and immune complexes was determined by changes in differential cell counts in the uterine lavage fluid and by histopathological examination of uterine tissue. the percentage of neutrophils was significantly (p l ...	1984	6241431
guillain-barré syndrome and campylobacter jejuni: a serological study.	the association between campylobacter jejuni infection and guillain-barré syndrome was investigated serologically in a retrospective study of 56 patients admitted to this hospital over four years. evidence of preceding c jejuni infection was found in 21 (38%) of these patients, indicating that c jejuni was the most common single identifiable pathogen precipitating the disease. among those patients who had presented with preceding diarrhoea the serum antibody response was similar to that in uncom ...	1984	6428580
premature labor and neonatal sepsis caused by campylobacter fetus, subsp. fetus--ontario.		1984	6431256
[campylobacter fetus meningitis in an adult male].		1984	6434669
economical, simple method for production of the gaseous environment required for cultivation of campylobacter jejuni.	campylobacter jejuni is an enteric pathogen recognized worldwide as a cause of diarrhea. its isolation from stool samples requires a microaerophilic environment that heretofore has been expensive and cumbersome to create. an economical, portable, and simple method is described which involves the production of appropriate concentrations of oxygen and carbon dioxide. inside a plastic bag are placed two cups, one containing fine steel wool (grade 0) previously soaked in a 2.5% aqueous solution of c ...	1984	6436301
spontaneous bacterial peritonitis due to campylobacter jejuni.	a patient with alcoholic cirrhosis complicated by ascites developed spontaneous bacterial peritonitis. campylobacter jejuni--(a microaerophilic bacillus)--was isolated from ascitic fluid and blood.	1984	6463002
campylobacter fetus subspecies jejuni in senegal.		1984	6468362
isoprenoid quinone content and cellular fatty acid composition of campylobacter species.	a total of 36 strains of campylobacter species were examined for isoprenoid quinones and cellular fatty acids. the isoprenoid quinone content was determined by reverse-phase high-pressure liquid chromatography, and the fatty acids were determined by capillary gas-liquid chromatography. all campylobacter species contained menaquinone-6 (2-methyl-3-farnesyl-farnesyl-1,4-naphthoquinone) and a methyl-substituted menaquinone-6 (2,[5 or 8]-dimethyl-3-farnesyl-farnesyl-1,4-napthoquinone) as the major i ...	1984	6470096
a new bacterial flagellar structure found in campylobacters.	a previously unrecognized bacterial flagellar structure present in campylobacter jejuni and campylobacter fetus is described. the structure is located just below the insertion of the flagellar apparatus through the bacterial wall. it is disc shaped, approximately 100 nm in diameter and has eleven spokes radiating from a central hole. the function of this new structure and its possible importance in the taxonomy of campylobacters and other spiral micro-organisms are discussed.	1984	6470668
new, extended biotyping scheme for campylobacter jejuni, campylobacter coli, and "campylobacter laridis".	a biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid h2s production, and dna hydrolysis was applied to 1,826 cultures of campylobacter jejuni, campylobacter coli and "campylobacter laridis" isolates from human and nonhuman sources. four biotypes were identified among c. jejuni: 57.3% of the isolates belonged to biotype i; 36.0%, to biotype ii; 4.0%, to biotype iii; and 2.7%, to biotype iv. c. coli organisms were differentiated into biotype i (67.0% ...	1984	6490850
a search for the source of campylobacter jejuni in milk.	samples of milk from 1501 cows with mastitis were negative for campylobacter jejuni. the faeces of 74 healthy friesian cows were screened for c. jejuni: 13% of the samples were positive during the summer when the cows were on pasture, and 51% were positive in the winter when the cows were housed. positive samples contained on average 1 x 10(4) campylobacters per g faeces. it is concluded that faecal contamination rather than udder infection is the means by which campylobacters enter milk and the ...	1984	6501879
quantitative evaluation of a transport-enrichment medium for campylobacter fetus.	the enrichment feature of a selective serum-based transport medium for campylobacter fetus was quantitatively examined. preputial samples from artificial insemination bulls were spiked with known numbers of c fetus strains and inoculated into transport-enrichment medium (tem). the survival and multiplication of these strains in tem under different incubation periods and temperatures were assessed by plate counts. mean enrichment values of 3.72 log and 4.42 log were observed after incubation at 3 ...	1984	6506432
comparison of antimicrobial susceptibility patterns of campylobacter jejuni and campylobacter coli.	to determine whether employing antibiograms is useful to separate campylobacter jejuni and campylobacter coli, we determined the mics of 12 antibiotics for 104 human clinical strains and 74 swine strains. of 74 swine strains, 5 (7%) were hippurate positive, as were 93 (89%) of 104 human strains. the 12 antimicrobial agents tested were ampicillin, amoxicillin, clindamycin, chloramphenicol, erythromycin, furazolidone, norfloxacin, nalidixic acid, rosoxacin, rosaramicin, tetracycline, and sch 32063 ...	1984	6508265
differential characteristics of catalase-positive campylobacters correlated with dna homology groups.	eighty-four strains of catalase-positive campylobacters could be placed into seven distinct dna homology groups (species), corresponding to campylobacter fetus, "c. hyointestinalis," c. jejuni, c. coli, "c. laridis," "c. fecalis," and aerotolerant campylobacters. the biochemical and physiological characteristics of the strains were examined for their correlation with the homology groups. the characterization tests that provided the most reliable differentiation at the species and subspecies leve ...	1984	6478314
susceptibility of campylobacter fetus subsp. jejuni to paromomycin.		1984	6489324
antibacterial activities of nitrothiazole against campylobacter jejuni and campylobacter coli.	niridazole (ambilhar) and three other newly synthesized nitrothiazole derivatives were highly active against 19 microaerophilic campylobacters (minimum concentration required to inhibit 50% of strains [mic50], 0.0075 to 0.015 mg/liter). there were, however, considerable differences in the susceptibility among strains tested, and one nitrothiazole derivative was rather inactive (mic50, 2 mg/liter). nitroimidazole derivatives, such as metronidazole and tinidazole, were less active (mic50, 2 and 4 ...	1984	6517542
campylobacter jejuni outer membrane proteins are antigenic for humans.	all campylobacter jejuni strains have a major outer membrane protein (omp) that migrates between a molecular weight of 41,000 (41k) and 45k and represents more than 50% of protein present, plus several more minor bands. using 125i-radiolabeled c. jejuni cells in a radioimmunoprecipitation procedure to assess whether the omps were antigenic, we studied serum from rabbits immunized with c. jejuni cells, from humans convalescent after c. jejuni infection, and from appropriate controls. in this assa ...	1984	6365789
analysis of campylobacter jejuni antigens with monoclonal antibodies.	to develop monoclonal reagents for antigenic analysis and serotyping of campylobacter spp., hybridoma cell lines were produced by fusion of mouse myeloma cells and spleen cells from mice immunized with formalin-treated campylobacter jejuni organisms. an enzyme immunoassay was used for preliminary screening of the cell culture supernatants and ascites. twenty-nine clones which reacted with the immunogen were obtained. seven of these clones were positive in passive hemagglutination tests with shee ...	1984	6365954
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?		1984	6368607
intestinal carriage of campylobacter jejuni and salmonella by chicken flocks at slaughter.	campylobacter jejuni were isolated in large numbers from the majority of birds sampled in colonic swabs from 28 of 60 flocks at slaughter. by contrast only small numbers of birds from 11 of the same 60 flocks yielded salmonella enteritidis serotypes. three c. jejuni isolates from each flock were serotyped on the basis of their heat-stable antigens, using antisera prepared against 16 serotypes common in campylobacter diarrhea in man. the majority (72 of 83) of the chicken isolates could be seroty ...	1984	6383579
comparison of enrichment methods and atmosphere modification procedures for isolating campylobacter jejuni from foods.	a comparison was made of enrichment broths for recovery of campylobacter jejuni from food by the methods of doyle and roman (appl. environ. microbiol. 43:1343-1353) and of park et al. (can. j. microbiol. 27:841-842). no significant differences were found between the results obtained with the two broths. recovery was greater, however, with a constant gas flow into the broths than with an evacuation-replacement method.	1984	6393876
isolation of campylobacter fetus ss jejuni in adult patients with diarrheal syndrome.		1984	6399196
detection of campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea.	rabbit antiserum, elicited by the intravenous injection of a strain of campylobacter jejuni heated to 100 degrees c, cross reacted strongly with all other thermophilic campylobacters tested as well as with "c pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. antisera to formalin killed cells did not do so. the correlation of positive stool culture with positive immunofluorescence of stools and rectal biopsies from patients with diarrhoea was 70-80%. some ...	1984	6381550
structure and cell envelope associations of flagellar basal complexes of vibrio cholerae and campylobacter fetus.	to isolate intact flagella with basal complexes from vibrio cholerae, a rhamnolipid hemolysin from pseudomonas aeruginosa was used to disrupt the cell envelope and flagellar sheath. the nonionic detergent, triton x-100, provided similar results for campylobacter fetus. each of these basal complexes possessed, in addition to the four classical rings, concentric membrane rings (cmr's) similar to those found in aquaspirillum serpens. through the use of stereo imaging (which allows structures to be ...	1984	6426766
[study of bacterial motility and rate of movement using a closed circuit television].	speed and motion patterns of campylobacter fetus ssp. jejuni, escherichia coli and pseudomonas aeruginosa were recorded using a closed circuit television camera attached to a phase contrast microscope. a sony video analysis system was used to stop frame videotape at 1/7th and 1/15th. bacterial speeds were: campylobacter 29.2 micron/s, e. coli 8.9 micron/s and p. aeruginosa 16.8 micron/s.	1984	6443639
a study of campylobacter fetus subsp. jejuni strains isolated from the stools of diarrhoeic children in sri lanka.	nine strains of campylobacter fetus subsp. jejuni were isolated from the stools of diarrhoeic children in colombo (sri lanka). the morphology of most of the strains was typical but three strains produced a spreading film of growth. no discrete domed colonies were seen. all were able to grow in a candle jar, and in a medium containing 1% glycine. all produced hydrogen sulphide. all were susceptible to ampicillin, carbenicillin, tetracycline, chloramphenicol, neomycin, erythromycin, gentamicin and ...	1984	6464104
campylobacter enteritis on hopi and navajo indian reservations. clinical and epidemiologic features.	from june 22 through september 30, 1981, stool specimens from 522 hopi and navajo outpatients were cultured because of diarrheal illnesses at the keams canyon indian health service hospital, arizona. campylobacter jejuni was isolated from the specimens of 26 (5%) of the patients. this pathogen was found as frequently as shigella in patients younger than 2 years or older than 20 years, but was significantly less common in the 2 to 20-year age group (p<.000001). campylobacter enteritis was indisti ...	1984	6475040
selected enrichment broths for recovery of campylobacter jejuni from foods.	we attempted to shorten the required time for enrichment broth culture for the isolation of campylobacter jejuni. enrichment broths described by doyle and roman and park and stankiewicz and one developed during this study were compared for ability to isolate c. jejuni from raw chicken carcasses. our medium was a modification of that of doyle and roman with the addition of filter-sterilized fbp (0.2% ferrous sulfate, 0.025% sodium metabisulfite, 0.05% sodium pyruvate), 0.1% sodium lauryl sulfate, ...	1984	6476833
campylobacter fetus ssp jejuni as seen in bangalore.		1984	6490113
heat injury and repair in campylobacter jejuni.	a procedure for detecting and quantitating heat injury in campylobacter jejuni was developed. washed cells of c. jejuni a7455 were heated in potassium phosphate buffer (0.1 m, ph 7.3) at 46 degrees c. samples were plated on brucella agar supplemented with na2s2o3, feso4 x 7h2o, and sodium pyruvate and on a medium containing brilliant green, bile, na2s2o3, feso4 x 7h2o, and sodium pyruvate. colonies were counted after 5 days of incubation at 37 degrees c in an atmosphere containing 5% o2, 10% co2 ...	1984	6497368
use of an ammonia electrode to study bacterial deamination of amino acids with special reference to d-asparagine breakdown by campylobacters.	a method using an ammonia electrode is being developed for investigating the deamination of amino acids and amides by bacteria. application of this method to campylobacter jejuni and c. coli has led to the demonstration of d-asparaginase activity in some strains. this has allowed the subdivision of both species into d-asparaginase-positive and -negative biotypes. even though the method is in the developmental stage, it was found to be generally reproducible and easy to perform. areas for further ...	1984	6501873
epidemiological investigations on campylobacter jejuni in households with a primary infection.	fifty-four rotterdam patients in which a primary infection with campylobacter jejuni had been detected (index patients) were compared with 54 control subjects with regard to the consumption and preparation of foods 7 days before onset of illness and the keeping of pet animals. significantly more index patients than controls had eaten chicken meat (47 v. 29; p = 0.0002), particularly at barbecues (14 v. 2; p = 0.0015). marginally more index patients had eaten pork (47 v. 39; p = 0.048) or inadequ ...	1984	6501878
improved biotyping schemes for campylobacter jejuni and campylobacter coli.	campylobacter jejuni (20 strains) and campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. the resulting biotyping schemes offer a greater degree of distinction among c. jejuni and c. coli strains than any of the other biotyping schemes previously described for these organisms.	1984	6511880
serotyping and serology studies of campylobacteriosis associated with consumption of raw milk.	a community outbreak of 15 cases of gastroenteritis was traced to consumption of unpasteurized milk produced at one commercial dairy. using two different testing schemes, we found that a campylobacter jejuni isolate from an ill patient and an isolate from a sick cow were the same serotype. bacteriological studies suggested that a single epidemic strain of campylobacter jejuni caused this outbreak.	1984	6511884
bacterial etiology of diarrhea in children with special reference to campylobacter fetus jejuni.		1984	6698587
composition of the antigenic material removed from campylobacter jejuni by heat.	the antigenic material removed form campylobacter jejuni by the boiling of whole cells in saline was examined biochemically. analyses showed that the extracted material contained 3 micrograms of protein per ml per mg of wet cells and ca. 2.6 micrograms of carbohydrate per ml per mg of wet cells. further extraction of the material with chloroform-methanol produced about 0.5 microgram of water-insoluble residue per ml per mg of wet cells, suggesting the presence of lipid as well. additional analys ...	1984	6520219
levels of some reproductive diseases in the dairy cattle of colombia.	of 4,144 serum samples collected from cows on 113 farms from eight areas of colombia 3.3% had positive and 8.8% inconclusive titres to brucella abortus, 21.7, 6.3, 1.6, 0.6 and 0.7% of cows had positive titres to leptospira serovars hardjo, pomona, canicola, icterohaemorrhagiae and grippotyphosa respectively. questionnaires completed on 110 farms revealed that 6, 2.5 and 4.6% of cows had had metritis, aborted or retained their placentas respectively in the previous 12 months. trichomonas foetus ...	1984	6523582
[campylobacter fetus subsp. jejuni: etiological, epidemiological and preventive aspects].	the authors, after a brief introduction, examine on the basis of the most recent literature, the taxonomy and the microbiological features of campylobacter fetus subsp. jejuni, giving a detailed description of the chemical composition, of the biochemical, physiological and nutritional characters, of the antigenic composition and of the cultural methods. later on, they state the problems arising from the epidemiology of the infections caused by this germ either in man or in animals, examining the ...	1984	6536547
improved preservation medium for campylobacter jejuni.	an egg-based medium was found to be superior to the conventional wang transport medium and the recently developed biphasic medium for the preservation of campylobacter jejuni in the laboratory. strains of c. jejuni preserved in egg-based medium maintained at 4 degrees c were viable for over 3 months. the survival of c. jejuni in egg-based medium held at room temperature (27 +/- 2 degrees c) was also relatively longer than in wang transport medium and biphasic medium.	1984	6699154
in vitro susceptibilities of 40 campylobacter fetus subsp. jejuni strains to niridazole and metronidazole.	the activities of niridazole and metronidazole were compared by an agar dilution method against 40 strains of campylobacter fetus subsp. jejuni of human origin. niridazole had a markedly higher activity than metronidazole.	1984	6703680
growth and survival characteristics of campylobacter jejuni in liquid egg.	growth and survival of four campylobacter jejuni strains in yolk, in liquid whole egg and in white during aerobic storage at 37, 20 and 4 degrees c was followed. in 48 h at 37 degrees c the cell counts of c. jejuni increased by about 3 log10 units in yolk and 1.60-3.35(10) log units in liquid whole egg. the growth of c. jejuni was slightly better in yolk than in liquid whole egg. at 20 degrees c during 48 h the cell counts decreased by about 0.5-1.5 log10 units in yolk and in liquid whole egg. a ...	1984	6537959
[rotavirus and campylobacter fetus jejuni associated with an outbreak of diarrhea in calves].	rotaviruses and campylobacter fetus jejuni are ubiquitous agents of diarrheal disease in animals and humans. under natural conditions they do not seem to cross inter-species barriers; a zoonosis has not been documented for man. however, animal rotaviruses might contribute to the emergence of new reassortment strains in view of their segmented genome, and thus, produce new antigenic variants. on the contrary, campylobacter fetus jejuni produces a true zoonosis. man acquires bacilli by ingesting w ...	1984	6545623
the effect of storage in liquid nitrogen on the recovery of human dental plaque bacteria.	centrifuged deposits of various individual bacteria from type-culture collections and dental plaque samples were immersed in liquid nitrogen for periods of up to 2 years. all the organisms were recovered after storage in liquid nitrogen but fusobacterium nucleatum (atcc 10953) and campylobacter fetus (nctc 10354) consistently showed a reduction in the number of colony-forming units after freezing. the main effect on bacterial proportions in plaque samples was a reduction in aerobic gram-negative ...	1984	6596040
hemolytic uremic syndrome after campylobacter-induced diarrhea in an adult.	campylobacter fetus subspecies jejuni is a recognized pathogen of the gastrointestinal (gi) tract resulting in a spectrum of illness from mild gastroenteritis to severe colitis with bloody diarrhea. campylobacter is also being recognized as capable of producing systemic illness. furthermore, antibody response, hypocomplementemia, and bacteremia with enterotoxic organisms have been described. many of the clinical features, both local (le, in the gi tract) and systemic, parallel those of shigella. ...	1984	6712399
comparative efficacy of liquid enrichment medium for isolation of campylobacter jejuni.	isolation of campylobacter jejuni from 1,249 human fecal specimens by direct inoculation on selective columbia agar and liquid enrichment medium was compared. the use of liquid enrichment medium provided a 30% higher isolation rate of c. jejuni. the overall isolation rate achieved by using direct plating and enrichment together was 8.2%.	1984	6715514
is enrichment culture necessary for the isolation of campylobacter jejuni from faeces?		1984	6725607
[an outbreak of campylobacter fetus subspecies jejuni enteritis in newborn infants in a maternity hospital].		1984	6742699
properties of crude campylobacter jejuni heat-labile enterotoxin.	the amount of crude campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure escherichia coli heat-labile toxin (lt) in the chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used gm ganglioside or antisera to lt or both. maximum c. jejuni enterotoxin production was achieved by growth at 42 degrees c for 24 h under agitation in supplemented gc medium. adding polymyxin separately to eithe ...	1984	6746090
dna relatedness among strains of campylobacter jejuni and campylobacter coli with divergent serogroup and hippurate reactions.	eleven strains of campylobacter from earlier fluorescent-antibody studies were examined by dna hybridization to determine their species. three of the strains hydrolyzed sodium hippurate, and eight did not. four of the hippurate-negative strains were in campylobacter jejuni serogroups, and the remaining strains were in both c. jejuni and campylobacter coli serogroups. dna relatedness to type strains of c. jejuni and c. coli indicated that all three of the hippurate-positive strains and two of the ...	1984	6746886
systematic investigation of enrichment media for wild-type campylobacter jejuni strains.	of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of campylobacter jejuni from cecal contents of chickens and chicken livers. it allowed the retrieval of 1 cfu as did solid media but permitted the screening of 50-times larger volumes. double-strength enrichment medium required 5 to 10 cfu for growth. omission of lauryl sulfate reduced the ...	1984	6746890
infection with campylobacter fetus.	over a 4-yr period (1978-1982) about 1 000 cases of campylobacter infection were diagnosed at our laboratory in helsinki, but only 4 proved to be due to campylobacter fetus. all these 4 patients had fever, 3 had diarrhoea and 1 arthritis and thrombophlebitis. the diagnosis was made from blood cultures in all cases; in 1 case the bacterium was also isolated from stools.	1984	6695155
endemic campylobacter jejuni infection in colorado: identified risk factors.	a study of persons with laboratory-confirmed sporadic campylobacter jejuni infection and of controls matched for age and sex in colorado in the summer of 1981 yielded odds ratio estimates significantly greater than 1 for the following risk factors: drinking raw water (10.74), drinking raw milk (6.93), eating undercooked chicken (2.77), and living in a household with a cat (3.21).	1984	6696155
campylobacter jejuni diarrhea model in infant chickens.	to study the pathogenic mechanisms of campylobacter jejuni infection, 36- to 72-h-old chickens were fed 10(3) to 10(6) live cells, using strains isolated from 40 patients with watery diarrhea and 6 with bloody mucoid diarrhea from whom no other known enteropathogen was detected. chickens of starbro strain were more likely to develop c. jejuni-induced diarrhea than were white leghorn chickens. diarrhea was defined on the basis of amounts of gut fluid in 288 chicks fed with live c. jejuni versus 1 ...	1984	6698612
serotyping and biotyping of campylobacter jejuni and campylobacter coli from sporadic cases and outbreaks in norway.	of 172 thermophilic campylobacters isolated from human cases of gastroenteritis in norway, 149 (86.6%) were classified as campylobacter jejuni, whereas 23 isolates (13.4%) belonged to campylobacter coli. c. jejuni biotype 1 comprised 66.3% and c. jejuni biotype 2 comprised 20.3% of the total number. using 50 unabsorbed antisera, we were able to serotype 109 (80.1%) of 136 campylobacters on the basis of heat-stable antigens identified by means of passive hemagglutination. the typable strains fell ...	1984	6699144
blood-free selective medium for isolation of campylobacter jejuni from feces.	a blood-free selective agar is described which contains charcoal, ferrous sulfate, sodium pyruvate, casein hydrolysates, cefazolin, and sodium deoxycholate (ccd agar). ccd agar was compared with preston medium for isolation of campylobacter jejuni from human feces, and isolation rates were similar on both media, but ccd agar was less selective. temperature studies at 37 and 42 degrees c confirmed that incubation of direct plates at 42 degrees c for 48 h was necessary for maximum isolation of c. ...	1984	6699146
[diarrhea caused by campylobacter fetus jejuni and other infective agents in children of the rural area of puriscal, costa rica].	between september 1979 and september 1981 a field study was conducted on the etiology of diarrheal disease in the area of puriscal, costa rica. the presence of enteric pathogens was investigated in the stools of 267 diarrheic children and 190 healthy controls. both groups belong to yearly cohorts recruited at birth as part of a longitudinal multidisciplinary study of mothers and children. campylobacter fetus jejuni was identified as the only pathogen in the stools of 24 diarrheic children (9%) a ...	1984	6535179
[frequency of campylobacter fetus ss. jejuni and yersinia enterocolitica in children with acute diarrhea].		1984	6704228
diagnostic studies of the fetus, placenta and maternal blood from 265 bovine abortions.	during 1981, the fetus, placenta and maternal serum were received from each of 265 bovine abortions. these specimens were examined using histopathological, histochemical, bacteriological, mycological, endocrinological, immunological, serological and virological techniques. the cause of abortion was identified in 98 (37%) cases. of these diagnosed abortions 27 (28%) were due to infection with fungi, 17 (17%) to salmonella spp, 11 (11%) to campylobacter fetus and 10 (10%) to corynebacterium pyogen ...	1984	6705541
association of campylobacter jejuni with laying hens and eggs.	laying hens were individually caged at 20 weeks of age and tested for fecal excretion of campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. peak rates of c. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in october and in late april to early may. before being segregated in late september, birds were allowed to consume fecal matter, litter, and communal drinking water, all likely sources of c. jejuni. the increased excr ...	1984	6712220
comparative studies on competitive exclusion of three isolates of campylobacter fetus subsp. jejuni in chickens by native gut microflora.	resistance of young chicks to campylobacter fetus subsp. jejuni was substantially increased by early exposure to native gut microflora. protection was demonstrated against two human isolates and a chicken isolate of c. fetus subsp. jejuni. significant protection against the chicken isolate was observed throughout a 91-day test period. infection reached 100% (25/25) in the untreated group at 56 days of age and only 4% (1/25) in the group treated with native gut microflora. campylobacter fetus sub ...	1984	6721792
a study of the spread of campylobacter jejuni in four large kitchens.	campylobacters were sought in swabs taken from work surfaces, sinks and floors of four kitchens-i.e. hospital, university, cook-freeze and commercial, processing frozen or fresh chickens. each kitchen was visited on four occasions. in the large commercial kitchen environmental contamination was found on each visit, whereas campylobacters were isolated on six of the twelve visits to the other kitchens. the hands of operatives were contaminated with campylobacters on only two of the 45 swabs taken ...	1984	6736643
[a case of septicemia by campylobacter fetus subsp. fetus].		1984	6737735
campylobacter septic abortion.	we have reported a case of a septic abortion caused by campylobacter fetus ssp jejuni. this organism is best known for producing diarrhea, but is now being linked with more serious diseases, including pregnancy wastage.	1984	6740362
factors affecting the lethality of campylobacter fetus subspecies jejuni in mice.	intraperitoneal injection of campylobacter fetus ss. jejuni into ham/1cr mice was lethal, but viable counts of bacteria from whole body homogenates, organs and blood indicated that death was not due to sustained bacterial multiplication. heat-killed organisms (5 x 10(9) cfu) injected into 7-day-old mice caused death within 24 h and this was shown to be due to endotoxin. both ferric iron and heterologous lipopolysaccharide enhanced virulence; the ld50 was lowered from 1.8 x 10(9) cfu to 2.7 x 10( ...	1984	6748038