Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| are low-density granulocytes the major target cells of classical swine fever virus in the peripheral blood? | the target cells of classical swine fever (csf) virus in the peripheral blood of pigs infected with recent field isolates from germany were studied. eight weaned pigs were inoculated oronasally with the csf virus field isolate visbek/han 95 and three weaners were inoculated with the isolate losten/freese 98. all pigs showed severe clinical signs typical of csf and died or had to be euthanized between 9 and 24 days post-infection (dpi). the first cells in the peripheral blood which became infecte ... | 2001 | 11708678 |
| the ns5a protein of bovine viral diarrhoea virus interacts with the alpha subunit of translation elongation factor-1. | a cellular protein that interacts with the ns5a polypeptide of bovine viral diarrhoea virus (bvdv) was identified in a yeast two-hybrid screen. the ns5a interactor was identified as the alpha subunit of bovine translation elongation factor 1a (eef1a). cell-free binding studies were performed with chimeric ns5a fused to glutathione s-transferase (gst-ns5a) expressed in bacteria. gst-ns5a bound specifically to both in vitro-translated and mammalian cell-expressed eef1a. moreover, purified eef1a bo ... | 2001 | 11714969 |
| cloning and sequencing of full-length cdna of classical swine fever virus lpc strain. | the cdnas of classical swine fever virus (lpc vaccine strain) were cloned by transcriptase-polymerase chain reaction, and their nucleotide sequences were determined. in this work, we obtained the sequence information of the 786 bases of the 5'-terminal region, 6049 bases of the middle region, and 1648 bases of the 3'-terminal region. taking our previous results and present data together, the entire genomic sequence of lpc strain was completed (12344 nucleotides in length). the genome of lpc has ... | 2001 | 11724273 |
| 1h, 15n, and 13c resonance assignments for a 20 kda dna polymerase from african swine fever virus. | 2001 | 11727982 | |
| mechanism of interferon resistance in hepatitis c. | 2001 | 11728538 | |
| the correlation of virus-specific interferon-gamma production and protection against classical swine fever virus infection. | the level of antigen-specific interferon-gamma (ifn-gamma) production can be used as an indicator of cellular immunity. in this study, we investigated the role of cellular immune response in protection against classical swine fever virus (csfv). pigs were vaccinated once with csfv vaccine and challenged 6 days post-vaccination (dpv). vaccinated animals had significantly higher csfv-specific ifn-gamma secreting cells than the unvaccinated pigs (p<0.05) at the time of challenge and were protected ... | 2001 | 11730928 |
| a serodiagnostic elisa using recombinant antigen of swine transmissible gastroenteritis virus nucleoprotein. | a serodiagnostic elisa utilizing the recombinant nucleoprotein (rn protein) of transmissible gastroenteritis virus (tgev) was developed, and evaluated by examining a panel of 141 virus neutralization (vn) positive and 101 negative sera. the rn protein-based elisa (rnelisa) appeared to be highly sensitive and specific (98.6% and 98.0%, respectively) when it was compared to the vn test. the result was similar to that of an elisa based on purified viral antigens with showing good correlation (r=0.8 ... | 2001 | 11767065 |
| evaluation of vaccine-induced maternal immunity against classical swine fever. | the vaccine-induced maternal immunity against classical swine fever (csf) was investigated in this study. eight sows were vaccinated with the chinese strain of classical swine fever virus (csfv). the length of time between vaccination and farrowing was 167-217 days. milk samples from the front, middle and back udder sections and blood samples were taken from the sows on days 3 and 14 after farrowing. blood samples were obtained from the piglets at the age of 3, 6 and 10 weeks. the antibody level ... | 2001 | 11402686 |
| [differential diagnosis of classical swine fever and border disease: seroepidemiological investigation of a pestivirus infection on a mixed sheep and swine farm]. | during recent years neutralizing antibodies against border disease virus (bdv) were found repeatedly in german pig herds. consequently there was a demand for a differential diagnostic system. a permanent sheep cell line and bdv reference strain moredun were chosen and were applied in a could be used case study. a pestivirus could be isolated from piglets on a mixed farm and was characterised as 'non-classical swine fever' (csf) by using monoclonal antibodies. due to a csf suspicion the pig herd ... | 2001 | 11417380 |
| atypical cilia in the bronchiolar epithelium of pigs experimentally infected with hog cholera virus. | to study the effect of hog cholera virus on the epithelial cells of the bronchiolar mucosa, 12 pigs were inoculated with a highly virulent strain. immunohistochemical and ultrastructural examination of the ciliated epithelial cells demonstrated an increase in the number of atypical cilia. the latter showed alterations in the microtubular pattern, possibly resulting from viral interference with the normal metabolism of the epithelial cells. | 2001 | 11428186 |
| transovarial transmission of african swine fever virus in the argasid tick ornithodoros moubata. | the aim of this study was to determine filial infection prevalence of experimentally infected colony ornithodoros moubata walton (ixodoidea: argasidae) ticks for african swine fever virus (asfv). three groups of ticks were used: an uninfected control group, one group orally infected with the vic t90/1 isolate and another group orally infected with the liv 13/33 isolate of asfv. the results show that filial infection prevalences were not constant but were highly variable between egg batches from ... | 2001 | 11434547 |
| african swine fever virus structural protein pe120r is essential for virus transport from assembly sites to plasma membrane but not for infectivity. | this report examines the role of african swine fever virus (asfv) structural protein pe120r in virus replication. immunoelectron microscopy revealed that protein pe120r localizes at the surface of the intracellular virions. consistent with this, coimmunoprecipitation assays showed that protein pe120r binds to the major capsid protein p72. moreover, it was found that, in cells infected with an asfv recombinant that inducibly expresses protein p72, the incorporation of pe120r into the virus partic ... | 2001 | 11435554 |
| classical swine fever: pathogenesis of glomerular damage and immunocharacterization of immunocomplex deposits. | twenty-six pigs were inoculated with a virulent isolate (quillota strain) of classical swine fever (hog cholera) virus to determine the chronological development of lesions in the renal glomeruli and the pathogenesis of glomerular damage and immunocomplex deposition. the study included the use of histopathological, ultrastructural and immunohistochemical (detection of viral antigen gp55, myeloid-histiocyte antigen, igm, igg and c1q) techniques. the main changes in glomerular structure were obser ... | 2001 | 11437500 |
| classical swine fever: morphological and morphometrical study of pulmonary intravascular macrophages. | to gain further insight into the pathogenesis of classical swine fever (csf), the changes induced by hog cholera (hc) virus in pulmonary intravascular macrophages (pims) were examined. twelve pigs were inoculated by the intramuscular route with a virulent strain of hc virus (quillota strain) and killed in groups of three at 4, 7, 10 and 14 days post-inoculation. immunohistochemical and ultrastructural examination revealed hc virus infection in endothelial cells, pims, and interstitial and alveol ... | 2001 | 11437510 |
| [african swine fever virus: achievements over the last decade of the 20th century]. | complete nucleotide sequence of african swine fever (asf) virus genome was determined in 1993-1999. deletion mutants with low virulence for pigs were obtained. genes of structural (p72, p54, p12, cleavage products pp220 and pp60, hemagglutinin) and nonstructural (p32) proteins were mapped. the significance of different proteins in virus adsorption and resistance to challenge was elucidated, their location in infected cell and virion was determined. lipid composition of the virus was studied. a p ... | 2001 | 11449798 |
| [genetic typing of german isolates of classical swine fever virus]. | during the last decade several outbreaks of classical swine fever (csf) occurred in germany in domestic pigs and in wild boar, respectively. two major epidemics which also affected other eu member states were recorded. to support epidemiological investigations genetic typing was applied and virus isolates originating from different outbreaks in germany were assigned to groups and virus types. two genomic regions were selected for the phylogenetic analysis, namely 150 nucleotides from the 5' non- ... | 2001 | 11449911 |
| a virally encoded chaperone specialized for folding of the major capsid protein of african swine fever virus. | it is generally believed that cellular chaperones facilitate the folding of virus capsid proteins, or that capsid proteins fold spontaneously. here we show that p73, the major capsid protein of african swine fever virus (asfv) failed to fold and aggregated when expressed alone in cells. this demonstrated that cellular chaperones were unable to aid the folding of p73 and suggested that asfv may encode a chaperone. an 80-kda protein encoded by asfv, termed the capsid-associated protein (cap) 80, b ... | 2001 | 11461995 |
| isolation of a non-haemadsorbing, non-cytopathic strain of african swine fever virus in madagascar. | african swine fever (asf) suspected clinically in madagascar (1998-9) was confirmed by polymerase chain reaction (pcr) and nucleotide sequencing, following virus isolation. no haemadsorption or cytopathic effect could be detected following leukocyte inoculation, but viral growth in cells was confirmed by pcr. detection of asf virus genome was carried out by amplification of a highly conserved region coding for the p72 protein. nucleotide sequencing of the amplicon revealed 99.2% nucleotide ident ... | 2001 | 11467803 |
| application of competitive enzyme-linked immunosorbent assay for the serologic diagnosis of classical swine fever virus infection. | a competitive enzyme-linked immunosorbent assay (c-elisa), based on a truncated e2 recombinant protein of the alfort/187 strain of classical swine fever virus (csfv) and a specific monoclonal antibody m1669, was evaluated using 2,000 sera from clinically healthy pigs in canada (a csfv-free country) and sera from experimentally infected pigs. the relative specificity and sensitivity of the c-elisa were 100% and 86%, respectively, at a cutoff of 25% inhibition using negative and positive pig sera, ... | 2001 | 11478613 |
| characterization of pseudotype vsv possessing hcv envelope proteins. | the genome of hepatitis c virus (hcv) encodes two envelope glycoproteins (e1 and e2), which are thought to be responsible for receptor binding and membrane fusion resulting in virus penetration. to investigate cell surface determinants important for hcv infection, we used a recombinant vesicular stomatitis virus (vsv) in which the glycoprotein gene was replaced with a reporter gene encoding green fluorescent protein (gfp) and produced hcv-vsv pseudotypes possessing chimeric hcv e1 or e2 glycopro ... | 2001 | 11485395 |
| evidence for the presence of two novel pestivirus species. | the genus pestivirus of the family flaviviridae comprises four species, namely bovine viral diarrhea virus-1 (bvdv-1), bvdv-2, border disease virus (bdv), and classical swine fever virus (csfv). comparative analyses of partial sequences have suggested that pestivirus isolates from giraffe (giraffe-1) and reindeer (reindeer-1) are distinct from the established species (becher et al., virology 262, 64--71, 1999). in this study, we report the complete genomic sequences of pestivirus strains giraffe ... | 2001 | 11485413 |
| novel virulence and host range genes of african swine fever virus. | current work is beginning to reveal the complex mechanisms by which african swine fever virus interacts with its swine and tick hosts. this work includes the identification of novel viral genes that mediate virulence and host range, and influence important cellular regulatory pathways. | 2001 | 11495811 |
| characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic rna isolated from wild boar. | classical swine fever virus (csfv) strain wb82, isolated from a wild boar in 1982, induced a distinct cytopathic effect (cpe) in primary swine testicle cell culture and in most of the porcine cell lines. this strain of csfv was found to be composed of two biotypes. cytopathogenic (cp) csfv, as a minor population, and noncytopathogenic (noncp) csfv, as a major population. the noncp csfv (designated strain wb82/e+) was obtained by biological cloning, and it showed the exaltation of newcastle disea ... | 2001 | 11503902 |
| classical swine fever (csf): a historical review of research and vaccine production on the isle of riems. | a review on classical swine fever (csf) research and vaccine production is given about four historical periods (1924-1948, 1949-1969, 1970-1991, since 1992). similar as to research on foot and mouth disease, applied topics as diagnosis, pathogenesis, epidemiology and control represented the csf research over many years. the development of vaccines and application procedures, e.g. oral and aerogenic immunisation and combined vaccines for large pig farms were the prominent investigations between 1 ... | 2001 | 11505796 |
| oral immunisation against classical swine fever (csf): onset and duration of immunity. | in an experimental study, onset and duration of immunity after oral immunisation of pigs with a classical swine fever (csf) live virus vaccine based on the strain "c" has been evaluated. sixteen weaner piglets (group 1) were orally instilled by syringe with the content of one vaccine bait whereas eighteen piglets (group 2) were fed with one bait. six unvaccinated piglets represented the control group (group 3). the pigs having 2, 4, 6 and 10 days post vaccination (p.v.) were challenged with the ... | 2001 | 11506924 |
| mutagenesis of hepatitis c virus e1 protein affects its membrane-permeabilizing activity. | the e1 glycoprotein of hepatitis c virus is a transmembrane glycoprotein with a c-terminal anchor domain. when expressed in escherichia coli, e1 induces a change in membrane permeability that is toxic to the bacterial cell. the c-terminal hydrophobic region (aa 331-383) of e1 is mainly responsible for membrane association and for inducing changes in membrane permeability. these observed changes are similar to those produced in e. coli by influenza virus m2, human immunodeficiency virus gp41 and ... | 2001 | 11514735 |
| evaluation of the potential of dogs, cats and rats to spread classical swine fever virus. | 2001 | 11548961 | |
| a recombinant classical swine fever virus with a marker insertion in the internal ribosome entry site. | based on an infectious cdna clone of classical swine fever virus (csfv) strain alfort/187 (ruggli et al., j virol 70, 3478-3487, 1996) a full-length cdna was constructed harbouring a nonviral 44 base insertion in the internal ribosome entry site (ires) within the 5' nontranslated region (5'ntr) of the genome. genome size rna transcribed in vitro served as a positive control in routine rt-pcr used to detect csfv rna in diagnostic material. unexpectedly this rna proved to be infectious upon transf ... | 2001 | 11556403 |
| attenuated lapinized chinese strain of classical swine fever virus: complete nucleotide sequence and character of 3'-noncoding region. | the complete nucleotide sequence including precise 5'- and 3'-terminal non-coding regions (ncrs) of the attenuated lapinized chinese strain (hclv) of classical swine fever virus (csfv) was determined from overlapping cdna clones constructed by separated rt-pcr and rapid amplification of cdna ends (race) methods. the genomic rna of the hclv strain consists of 12.310 nucleotides (nts) including 374 nts and 242nts in the 5'- and 3'-ncrs, respectively. it contains one large open reading frame (orf) ... | 2001 | 11556404 |
| classical swine fever (csf) marker vaccine. trial i. challenge studies in weaner pigs. | two commercial marker vaccines against classical swine fever virus (csfv) and companion diagnostic tests were examined in 160 conventional pigs. to test the vaccines in a "worst case scenario", group of 10 weaners were vaccinated using a single dose of an e2 (gp55) based vaccine at days -21, -14, -10 or -7, and subsequently challenged at day 0. the challenge virus was csfv 277, originating from a recent outbreak of classical swine fever (csf) in germany. in all groups, only 5 out of 10 pigs were ... | 2001 | 11557151 |
| classical swine fever (csf) marker vaccine. trial ii. challenge study in pregnant sows. | the efficacy of two marker vaccines against classical swine fever (csf) was tested in a large scale laboratory trial in several national swine fever laboratories (nsfl) of the eu member states. the vaccines were: bayovac csf marker (vaccine a) from bayer, leverkusen, germany and porcilis pesti (vaccine b) from intervet, boxmeer, the netherlands. at the nsfl of belgium, the netherlands and germany experiments were carried out to examine the ability of the vaccines to prevent transplacental transm ... | 2001 | 11557152 |
| classical swine fever (csf) marker vaccine. trial iii. evaluation of discriminatory elisas. | the objective of the marker vaccine trial was to test the two available csf marker vaccines in scenarios which are likely to occur in the field and to evaluate the reliability of the discriminatory tests. the evaluation of the discriminatory tests was of special importance because there is no requirement for formal data concerning their performance by the european medicinal products evaluation agency (emea) in london. emea is responsible for the licensing procedure of the marker vaccines within ... | 2001 | 11557153 |
| interaction of classical swine fever virus with membrane-associated heparan sulfate: role for virus replication in vivo and virulence. | passage of native classical swine fever virus (csfv) in cultured swine kidney cells (sk6 cells) selects virus variants that attach to the surface of cells by interaction with membrane-associated heparan sulfate (hs). a ser-to-arg change in the c terminus of envelope glycoprotein e(rns) (amino acid 476 in the open reading frame of csfv) is responsible for selection of these hs-binding virus variants (m. m. hulst, h. g. p. van gennip, and r. j. m. moormann, j. virol. 74:9553-9561, 2000). in this i ... | 2001 | 11559790 |
| african swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein. | dynein is a minus-end-directed microtubule-associated motor protein involved in cargo transport in the cytoplasm. african swine fever virus (asfv), a large dna virus, hijacks the microtubule motor complex cellular transport machinery during virus infection of the cell through direct binding of virus protein p54 to the light chain of cytoplasmic dynein (lc8). interaction of p54 and lc8 occurs both in vitro and in cells, and the two proteins colocalize at the microtubular organizing center during ... | 2001 | 11559815 |
| an e2 sub-unit marker vaccine does not prevent horizontal or vertical transmission of classical swine fever virus. | an experimental infection with classical swine fever (csf) virus in e2 sub-unit marker vaccine vaccinated gilts was conducted in order to evaluate the effect of vaccination on virus transmission and course of the disease. therefore, clinical signs as well as horizontal and vertical virus transmission were monitored in two inoculated, non-vaccinated and 10 vaccinated conventional gilts, housed in individual sow boxes. within 10 days post-inoculation, all vaccinated gilts became infected. dependin ... | 2001 | 11567750 |
| molecular epidemiology of classical swine fever in italy. | to gain an insight into the molecular epidemiology of classical swine fever (csf) in italy, virus isolates originating from outbreaks that occurred between 1985 and 2000 in wild boar or in domestic pigs in mainland italy and in sardinia were analysed by genetic typing. for this, a fragment (190 nucleotides) of the e2 glycoprotein gene was sequenced and phylogenetic analyses were performed, including older italian isolates and isolates from recent outbreaks in europe for comparison. the results s ... | 2001 | 11574170 |
| morphological and immunohistochemical changes in splenic macrophages of pigs infected with classical swine fever. | classical swine fever (csf) was induced in 20 pigs by inoculation with a virulent strain of csf virus to determine sequential changes (2, 4, 7, 10 and 14 days post-inoculation) in the number and morphology of splenic macrophages (red pulp and lymphoid marginal zone) and thus to assess the role of these cells in the pathogenesis of the disease. the first splenic cells to be infected with csf virus were macrophages in the marginal zone followed by other macrophage populations. the initial phase of ... | 2001 | 11578124 |
| evaluation of diagnostic tests for the detection of classical swine fever in the field without a gold standard. | knowledge of the sensitivity of diagnostic tests for infectious diseases under field conditions can be used to design a surveillance program that increases the effectiveness of the control policy. in this study, the sensitivity of tests for the detection of classical swine fever (csf) virus (csfv) under field conditions was estimated without knowledge of the true disease status of the animals tested. during the csf epidemic of 1997-1998 in the netherlands, tonsil samples from pigs of csf suspect ... | 2001 | 11580058 |
| african swine fever virus infection of porcine aortic endothelial cells leads to inhibition of inflammatory responses, activation of the thrombotic state, and apoptosis. | african swine fever (asf) is an asymptomatic infection of warthogs and bushpigs, which has become an emergent disease of domestic pigs, characterized by hemorrhage, lymphopenia, and disseminated intravascular coagulation. it is caused by a large icosohedral double-stranded dna virus, african swine fever virus (asfv), with infection of macrophages well characterized in vitro and in vivo. this study shows that virulent isolates of asfv also infect primary cultures of porcine aortic endothelial cel ... | 2001 | 11581405 |
| erns protein of pestiviruses. | 2001 | 11586914 | |
| factors associated with the introduction of classical swine fever virus into pig herds in the central area of the 1997/98 epidemic in the netherlands. | a matched case-control study of 135 infected and 99 uninfected pig herds from the central area of the 1997 to 1998 epidemic of classical swine fever (csf) in the netherlands was undertaken to identify factors associated with the introduction of the virus. the herds were matched on the basis of herd type and the shortest geographical distance between pairs of herds. data on management, hygienic measures, experiences during the depopulation of an infected nearest neighbour, and the frequency of co ... | 2001 | 11601514 |
| african swine fever virus protease, a new viral member of the sumo-1-specific protease family. | african swine fever virus (asfv) is a complex dna virus that employs polyprotein processing at gly-gly-xaa sites as a strategy to produce several major core components of the viral particle. the virus gene s273r encodes a 31-kda protein that contains a "core domain" with the conserved catalytic residues characteristic of sumo-1-specific proteases and the adenovirus protease. using a cos cell expression system, it was found that protein ps273r is capable of cleaving the viral polyproteins pp62 an ... | 2001 | 11031264 |
| identification and characterization of a spliced c-type lectin-like gene encoded by rat cytomegalovirus. | the english isolate of rat cytomegalovirus (rcmv) encodes a 20-kda protein with a c-type lectin-like domain that is expressed in the delayed-early and late phases of the viral replication cycle. genomic sequence analysis of the restriction fragment kpnr of rcmv revealed significant homology to several c-type lectin-containing molecules implicated in natural killer (nk) and t-cell interactions, as well as genes from four poxviruses and african swine fever virus. the gene is spliced into five exon ... | 2001 | 11134273 |
| african swine fever virus iap homologue inhibits caspase activation and promotes cell survival in mammalian cells. | african swine fever virus (asfv) a224l is a member of the inhibitor of apoptosis protein (iap) family. we have investigated the antiapoptotic function of the viral iap both in stably transfected cells and in asfv-infected cells. a224l was able to substantially inhibit caspase activity and cell death induced by treatment with tumor necrosis factor alpha and cycloheximide or staurosporine when overexpressed in vero cells by gene transfection. we have also observed that asfv infection induces caspa ... | 2001 | 11222676 |
| secretory pathway limits the enhanced expression of classical swine fever virus e2 glycoprotein in insect cells. | the 3' untranslated region (utr) is an important element that determines the level of recombinant protein expression via baculovirus vectors. previous work using chloramphenicol acetyl transferase as reporter has shown that p10-promoter based baculovirus vectors with the authentic p10 3' utr resulted in higher expression levels than vectors carrying an sv40 early terminator, as part of a lacz selection cassette. to examine whether a similar increase in expression levels could be obtained for bac ... | 2001 | 11223142 |
| enzyme-linked immunosorbent assay using a virus type-specific peptide based on a subdomain of envelope protein e(rns) for serologic diagnosis of pestivirus infections in swine. | peptides deduced from the c-terminal end (residues 191 to 227) of pestivirus envelope protein e(rns) were used to develop enzyme-linked immunosorbent assays (elisas) to measure specifically antibodies against different types of pestiviruses. the choice of the peptide was based on the modular structure of the e(rns) protein, and the peptide was selected for its probable independent folding and good exposure, which would make it a good candidate for an antigenic peptide to be used in a diagnostic ... | 2001 | 11230402 |
| african swine fever virus multigene family 360 and 530 genes are novel macrophage host range determinants. | pathogenic african swine fever virus (asfv) isolates primarily target cells of the mononuclear-phagocytic system in infected swine and replicate efficiently in primary macrophage cell cultures in vitro. asfvs can, however, be adapted to grow in monkey cell lines. characterization of two cell culture-adapted viruses, ms16 and ba71v, revealed that neither virus replicated in macrophage cell cultures. cell viability experiments and ultrastructural analysis showed that infection with these viruses r ... | 2001 | 11238833 |
| evaluation of genetic vaccine against classical swine fever. | classical swine fever is important diseases affecting pigs. it results in great losses in their population and in limitations in the commercial international trade of pigs. the aim of the study was the preparation of the genetic vaccine against csf and the estimation of its safety, protection value and immunogenicity. clinical observations, body temperature and the immune response (haematological and facs analyses) were monitored. pigs vaccinated with the dna vaccine were protected from the chal ... | 2001 | 11257381 |
| evolution of hepatitis c virus genome in chronically infected patients receiving ribavirin monotherapy. | recent results of clinical trials suggest that combination of interferon and ribavirin exhibits an enhanced antiviral effect in the treatment of chronic hepatitis c. to investigate the effect of ribavirin on hepatitis c virus (hcv) infection, we analysed the evolution of the genetic heterogeneity of hcv in relation to the anti-hcv humoral response in patients treated by ribavirin alone. the study population included 35 patients with liver biopsy proven chronic hepatitis c infected with hcv genot ... | 2001 | 11264732 |
| immunogenicity of the e1e2 proteins of hepatitis c virus expressed by recombinant adenoviruses. | the e1 and e2 proteins of hepatitis c virus (hcv) are believed to be the viral envelope glycoproteins that are major candidate antigens for hcv vaccine development. we reported previously that the replication-competent recombinant adenovirus encoding core-e1-e2 genes of hcv (ad/hcv) produces serologically reactive e1 and e2 proteins forming a heterodimer in substantial amounts. here, we examined immunogenicity of the e1e2 proteins copurified from hela cells infected with ad/hcv virus in mice. fu ... | 2001 | 11282207 |
| a prime-boost vaccination strategy using naked dna followed by recombinant porcine adenovirus protects pigs from classical swine fever. | weaned pigs (6-week-old) and 7-day-old pre-weaned piglets were vaccinated with naked plasmid dna expressing the gp55/e2 gene from classical swine fever virus (csfv). both groups of pigs were then given a booster dose of recombinant porcine adenovirus expressing the gp55 gene (rpav-gp55). following challenge with csfv, 100% of weaned pigs and 75% pre-weaned piglets were protected from disease. weaned pigs given a single dose of rpav-gp55 were also protected, but showed a slight increase in temper ... | 2001 | 11295331 |
| vitamin b12 and hepatitis c: molecular biology and human pathology. | cobalamins are stored in high concentrations in the human liver and thus are available to participate in the regulation of hepatotropic virus functions. we show that cyanocobalamin (vitamin b12) inhibited the hcv internal ribosome entry site (ires)-dependent translation of a reporter gene in vitro in a dose-dependent manner without significantly affecting the cap-dependent mechanism. vitamin b12 failed to inhibit translation by ires elements from encephalomyocarditis virus (emcv) or classical sw ... | 2001 | 11296247 |
| construction of recombinant swinepox viruses and expression of the classical swine fever virus e2 protein. | to explore the swinepox virus (spv) as a potential live vector for immunization, a vector was developed for the construction of a recombinant spv carrying foreign genes. in this system, a foreign gene placed under the strong vaccinia virus promoter p(11) can be inserted into the viral thymidine kinase (tk) gene, and the recombinant virus can be isolated in a non-selective medium by the co-expression of e. coli lacz gene. compared with the wild type virus, the tk(-)recombinant spv showed a modest ... | 2001 | 11311343 |
| descriptive epidemiology of a classical swine fever outbreak in the limburg province of belgium in 1997. | this paper describes the epidemiological characteristics of the 1997 classical swine fever (csf) outbreak that occurred in the limburg province of belgium, where there is a policy of non-vaccination, intensive surveillance and eradication. between 30 june and 17 july 1997, eight herds, located in three different areas, were confirmed to be csf-positive. csf virus was transmitted from the primary infected herd of one area to another five herds in the same area and to one herd in a different area. ... | 2001 | 11315525 |
| aggresomes resemble sites specialized for virus assembly. | the large cytoplasmic dna viruses such as poxviruses, iridoviruses, and african swine fever virus (asfv) assemble in discrete perinuclear foci called viral factories. factories exclude host proteins, suggesting that they are novel subcellular structures induced by viruses. novel perinuclear structures, called aggresomes are also formed by cells in response to misfolded protein (johnston, j.a., c.l. ward, and r.r. kopito. 1998. j. cell biol. 143:1883--1898; garcía-mata, r., z. bebök, e.j. sorsche ... | 2001 | 11331297 |
| the influence of downstream protein-coding sequence on internal ribosome entry on hepatitis c virus and other flavivirus rnas. | some studies suggest that the hepatitis c virus (hcv) internal ribosome entry site (ires) requires downstream 5' viral polyprotein-coding sequence for efficient initiation of translation, but the role of this rna sequence in internal ribosome entry remains unresolved. we confirmed that the inclusion of viral sequence downstream of the aug initiator codon increased ires-dependent translation of a reporter rna encoding secretory alkaline phosphatase, but found that efficient translation of chloram ... | 2001 | 11345437 |
| classical swine fever virus (c strain) distribution in organ samples of inoculated piglets. | enzyme linked immunosorbent assays (elisas) and a nested polymerase chain reaction after reverse transcription (rt-pcr) were used for the detection of the chinese strain (c strain) of classical swine fever virus (csfv) in blood and tissue samples of experimentally inoculated piglets. one group of 10 piglets was inoculated with c strain material from rabbits and a second one with material from infected minipig kidney (mpk) cell culture. tested blood samples were taken on the day of inoculation as ... | 2001 | 11356313 |
| [detection of infection with classical swine fever virus in wild boar: a comparison of different laboratory diagnostic methods]. | the aim of this study was to evaluate two commercially available elisas for routine diagnosis of classical swine fever virus (csfv) in wild boar. for this, 222 tissue samples from wild boar were tested in the elisas and the results were compared to those obtained using standard methods. first, frozen spleen sections were examined by direct immunofluorescence, and organ suspensions were prepared and tested for csfv antigen samples were simultaneously examined with the chekit-elisa (dr. bommeli ag ... | 2001 | 11367881 |
| induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus. | the pathogenesis of bone marrow atrophy during classical swine fever (csf) was investigated in vitro by using csf virus (csfv) infection of bone marrow haematopoietic cells (bmhc). the monocytic lineage had the highest susceptibility to csfv infection, whereas the more mature swc8(+) granulocytic cells were not directly susceptible to infection. however, myelomonocytic precursors were targets for csfv infection and continued to differentiate into swc8(+) granulocytic cells, which remained infect ... | 2001 | 11369874 |
| highly sensitive one-tube rt-pcr and microplate hybridisation assay for the detection and for the discrimination of classical swine fever virus from other pestiviruses. | rapid, sensitive and specific laboratory diagnostic methods are necessary to confirm outbreaks of classical swine fever. the detection of classical swine fever virus (csfv) and its discrimination from other pestiviruses can be achieved by virus isolation on cell culture, antigen detection, or molecular methods. to reduce the time and the number of steps in the diagnostic procedure a sensitive and rapid detection method based on specific amplification of the pestiviral rna by one-step reverse tra ... | 2001 | 11377717 |
| [phylogenetic analysis of swine fever virus strains]. | amplification of h-gene fragment in combination with cdna nucleotide sequencing can be used for indication and strain differentiation of classical swine fever virus. | 2001 | 11392970 |
| oral immunization of pigs against classical swine fever. course of the disease and virus transmission after simultaneous vaccination and infection. | the efficacy of simultaneous vaccination of pigs against classical swine fever (csf) and challenge was evaluated. in this study, domestic weanling pigs were vaccinated orally with a conventional live virus vaccine based on csf virus (csfv) c strain and were challenged simultaneously with csfv of different virulence. all the animals vaccinated and challenged with a high dose of highly virulent koslov strain died while three of five animals challenged with a low dose of highly virulent alfort 187 ... | 2001 | 11394574 |
| african swine fever virus-induced polypeptides in porcine alveolar macrophages and in vero cells: two-dimensional gel analysis. | high-resolution two-dimensional electrophoresis followed by computer analysis has been used to study quantitatively the patterns of protein synthesis produced in porcine alveolar macrophages and in vero cells infected with african swine fever virus (asfv). initially, a protein database for each cell type was constructed. the porcine alveolar macrophage database includes 995 polypeptides (818 acidic, isoelectric focusing (ief) and 177 basic, nonequilibrium ph gradient electrophoresis (nephge)) wh ... | 2001 | 11922604 |
| [sero-monitoring of notifiable diseases in wild boar in the netherlands 1999-2001]. | within the framework of a sero-monitoring system, in operation since 1996. blood samples from wild boar shot during the hunting seasons 1999-2000 and 2000-2001 in the netherlands were screened for the presence of antibodies against classical swine fever virus (csfv), swine vesicular disease virus (svdv), and anjeszky's disease virus (adv). the results indicate that csfv, svdv, and adv are uncommon in the wild boar population in the netherlands. because of the recent foot-and-mouth disease (fmd) ... | 2001 | 11780256 |
| a 250-nucleotide ua-rich element in the 3' untranslated region of xenopus laevis vg1 mrna represses translation both in vivo and in vitro. | xenopus laevis vgl mrna undergoes both localization and translational control during oogenesis. vg1 protein does not appear until late stage iv, after localization is complete. to determine whether vg1 translation is regulated by cytoplasmic polyadenylation, the race-pat method was used. vg1 mrna has a constant poly(a) tail throughout oogenesis, precluding a role for cytoplasmic polyadenylation. to identify cis-acting elements involved in vg1 translational control, the vg1 3' utr was inserted do ... | 2001 | 11780632 |
| pathogenesis of african swine fever virus in ornithodoros ticks. | african swine fever virus (asfv) is the only known dna arbovirus and the sole member of the family asfarviridae. it causes a lethal, hemorrhagic disease in domestic pigs. asfv is enzootic in sub-saharan africa and is maintained in a sylvatic cycle by infecting both wild members of the suidae (e.g. warthogs) and the argasid tick ornithodoros porcinus porcinus. the pathogenesis of asfv in o. porcinus porcinus ticks is characterized by a low infectious dose, lifelong infection, efficient transmissi ... | 2001 | 11831434 |
| the effect of vaccination with the pav-250 strain classical swine fever (csf) virus on the airborne transmission of csf virus. | the airborne transmission of classical swine fever (csf) virus to susceptible pigs, as well as the effect of vaccination with the csf virus pav-250 strain was investigated on this mode of transmission. experiment i: four pigs were inoculated with the ald csfv strain (10(4.3) 50% tcid) by the intramuscular route, and at the onset of fever, they were introduced into an enclosed chamber. at the end of the experiment surviving pigs were sedated, anesthetized and euthanatized. experiment ii: four pig ... | 2001 | 11642288 |
| phylogenetic comparison of classical swine fever virus in china. | an n-terminal fragment of the e2 gene of classical swine fever (csf) virus encoding major immunogenic sites was amplified by rt-pcr directly from 110 clinical specimens representing 109 epizootic sites during the last decade in china. phylogenetic relationships between these viruses as well as 20 reference strains were determined by comparison of their nucleotide sequences. a phylogenetic tree showed that 103 of the 110 field viruses (93.6%) were clustered within group 2 and subdivided into thre ... | 2001 | 11682122 |
| epidemiology of the 2000 csf outbreak in east anglia: preliminary findings. | 2001 | 12503600 | |
| [sequencing of e2 gene and comparison analysis of four strains hog cholera virus (hcv)]. | four cdna fragments of envelope glycoprotoin e2 gene of sm strain, hclv strain, f03 strain and f07 strain were amplified respectively with rt-pcr method. the amplified e2 fragments of four hcv strains were all 1273 bp in length by agarose gel electrophoresis. four e2 fragments were cloned respectively into pgem-t easy vector. 1273 bp cdna fragment of four hcv e2 gene were sequenced and 381 residues amino acid sequence of e2 glycoprotein were deduced. the signal peptide sequence (wlllvtga) locate ... | 2001 | 12549086 |
| [construction of the full-length cdna clone of chinese virulent strain--f114]. | seven subclones covered the complete genome of classical swine fever virus chinese virulent strain f114 were obtained by reverse transcription pcr. the complete nucleotide sequence of the genome of strain f114 was determined by sequencing. the cdna fragments were then assembled and inserted downstream of a t7 promoter in pbluescript ii sk+ plasmid vector to obtain the full-length cd-na clone sk-12297. homology comparison of the nucleotide and amino acid sequence of strain f114 with the known seq ... | 2001 | 12552911 |
| [cloning and sequence analysis of e0 gene of hog cholera virus lapinized chinese strain and virulent shimen strain]. | according to the published nucleotide sequences of genome of hog cholera virus, one pair of specific primers were designed and synthesized. from the spleen of rabbits which were infected with hclv and hcv shimen strain infected pig blood, the two e0 genes were amplified by rt-pcr. the amplified fragments were cloned into pgem-t vector and sequenced. sequence analysis showed nucleotide sequence and deduced amino acid sequence homologies of the e0 gene between hclv and shimen strain were 95.0% and ... | 2000 | 12548875 |
| [study on the morphological processing of classical swine fever virus in cultured cells]. | an infected mode between the thiverval strain and chinese strain of csfv and mpk cells are established. the morphological structure and processing of different strains(t strain, c strain and f strain) of csfv were studied by ultra-thin section electron microscopy. the virions of csfv are roughly round and approximately 70 nm in diameter with a 40 nm core, and are wraped by membrane. the distinctive pathway of the maturation and release of csfv are observed. virons on different deverloping state ... | 2000 | 12548986 |
| [molecular cloning and expression of e2 gene of the chinese classical swine fever virus(shimen strain) and preliminary studies of its dna vaccine]. | a 1.1 bp fragment of e2 gene of chinese classical swine fever virus(csfv) shimen strain, a standard virulent strain, was amplified by rt-pcr from total rna of cell cultures infected by csfv, and cloned into pgem t vector. the nucleotide sequence of this fragment was sequenced by sanger's method and the amino acid sequence was deduced. compared with the corresponding region of alfort, brescia and c strain of csfv, the nucleotide sequence homology is 84.7%, 92.6% and 95.2% respectively, and the am ... | 2000 | 12548987 |
| [nucleotide sequence analysis of e2 major protective antigen encoding region of 12 strains of hog cholera virus(hcv)]. | cdna fragments, of hcv envelope glycoprotein e2 major gene of 11 field strains isolated in china in different time and 1 french reference strain(thiveral) were amplified respectively with rt-pcr method and sequenced. the fragments amplified located by the 5' 2485 to 2708 of e2 major domains b and c and encoded 75 amino acid residues of e2 glycoprotein. all the products amplifield by rt-pcr from 12 strains in the study were same size of 224 bp. comparing 12 sequences with other 9 references strai ... | 2000 | 12549056 |
| relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex. | thirty-nine healthy pigs (28-32 days old) were purchased from a commercial swine farm and housed at swine pens of the college. the animals were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (hcv, lom strain) and then boostered at 5 weeks after the first vaccination. the animals were divided into 4 experimental groups: 0.05% (w/w) powerfeel-supplemented diet (t-1, n = 10); 3% (w/w) superfeed-supplemented diet (t-2, n = 10); diluted powerfeel solution (1 : 500, v/v) a ... | 2000 | 14612620 |
| effects of classical swine fever virus infection on the porcine leukocyte subsets. | the effects of classical swine fever (csf) virus infection on the porcine leukocyte subsets were investigated by flow cytometry in acute, chronic and convalescent forms of the disease. the virus antigen could be first detected in the monocytes on postinfection (p.i.) day 10 while in the lymphocytes on p.i. day 13. it could be established that the ratio of cd6+ cells decreased until p.i. day 6, but afterwards it started to increase and reached different values. the cd4+cd8+, the cd8+ and the cd6- ... | 2000 | 11402673 |
| ribosomal binding to the internal ribosomal entry site of classical swine fever virus. | most eukaryotic mrnas require the cap-binding complex elf4f for efficient initiation of translation, which occurs as a result of ribosomal scanning from the capped 5' end of the mrna to the initiation codon. a few cellular and viral mrnas are translated by a cap and end-independent mechanism known as internal ribosomal entry. the internal ribosome entry site (ires) of classical swine fever virus (csfv) is approximately 330 nt long, highly structured, and mediates internal initiation of translati ... | 2000 | 11142379 |
| classical swine fever virus: clinical, virological, serological and hematological findings after infection of domestic pigs and wild boars with the field isolate "spante" originating from wild boar. | a classical swine fever virus (csfv) field isolate originating from wild boar was investigated on its virulence in domestic pigs and wild boar. three weaner pigs and two wild boars (yearlings) were intranasally inoculated with the isolate "spante" and tested for clinical, virological, hematological and serological findings until day 31 after infection (p. i.). one day p. i. the piglets were put in contact to three sentinel pigs. during a period of 31 d neither the domestic pigs nor the wild boar ... | 2000 | 11153219 |
| vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (e2) gene protects pigs against classical swine fever. | a recombinant porcine adenovirus (rpav) with the gp55 (e2) gene from the classical swine fever virus (csfv) 'weybridge' strain inserted into the right hand end of the pav serotype 3 (pav3) genome was constructed. expression of gp55 was directed by the major late promoter and tri-partite leader sequences located and cloned from pav3. no compensatory deletions of pav dna sequences were made. vaccination of outbred pigs with a single dose of the recombinant virus (rpav-gp55) resulted in complete pr ... | 2000 | 10590324 |
| an african swine fever virus erv1-alr homologue, 9gl, affects virion maturation and viral growth in macrophages and viral virulence in swine. | the african swine fever virus (asfv) genome contains a gene, 9gl, with similarity to yeast erv1 and alr genes. erv1 has been shown to function in oxidative phosphorylation and in cell growth, while alr has hepatotrophic activity. 9gl encodes a protein of 119 amino acids and was highly conserved at both nucleotide and amino acid levels among all asfv field isolates examined. monospecific rabbit polyclonal antibody produced to a glutathione s-transferase-9gl fusion protein specifically immunopreci ... | 2000 | 10627538 |
| regulation of calcineurin by growth cone calcium waves controls neurite extension. | growth cones generate spontaneous transient elevations of intracellular ca(2+) that regulate the rate of neurite outgrowth. here we report that these ca(2+) waves inhibit neurite extension via the ca(2+)-dependent phosphatase calcineurin (cn) in xenopus spinal neurons. pharmacological blockers of cn (cyclosporin a and deltamethrin) and peptide inhibitors of cn [the xenopus cn (xcn) autoinhibitory domain and african swine fever virus protein a238l] block the ca(2+)-dependent reduction of neurite ... | 2000 | 10627609 |
| african swine fever virus ep153r open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells. | the open reading frame ep153r, located within the ecori e' fragment of the african swine fever (asf) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the n-terminal region of several cd44 molecules. ep153r contains multiple putative sites for n-glycosylation, phosphorylation, and myristoylation, a central transmembrane region, a c-type animal lectin-like domain, and a cell attachment sequence. transcription of ep153r takes place at ... | 2000 | 10639320 |
| characterization of the african swine fever virus protein p49: a new late structural polypeptide. | the open reading frame b438l, located within the ecori b fragment of the african swine fever virus genome, is predicted to encode a protein of 438 amino acids with a molecular mass of 49.3 kda. it presents a cell attachment rgd (arg-gly-asp) motif but no other significant similarity to protein sequences in databases. northern blot and primer extension analysis showed that b438l is transcribed only at late times during virus infection. the b438l gene product has been expressed in escherichia coli ... | 2000 | 10640542 |
| determination of the onset of the herd-immunity induced by the e2 sub-unit vaccine against classical swine fever virus. | for a recently developed e2 subunit vaccine against classical swine fever (csf), the reduction in transmission, at different moments after vaccination, was assessed by animal experiments and statistical calculations. two experiments were performed to estimate the reproduction ratio r. experiment 1 consisted of three groups and experiment 2 of two groups each of 10 pigs. in four of these groups, all pigs were vaccinated intramuscularly with the vaccine. the pigs in the fifth group remained unvacc ... | 2000 | 10618535 |
| origins of the csf outbreak. | 2000 | 11037735 | |
| molecular epidemiology of a large classical swine fever epidemic in the european union in 1997-1998. | a big epidemic of classical swine fever (csf) occurred in the european community in 1997. the first case was reported at the beginning of january 1997 from germany. the disease presumably spread to the netherlands, and from there to italy, spain and eventually to belgium. about 30 isolates from these outbreaks were analysed by comparison of the nucleotide sequence data generated from fragments of both the e2 glycoprotein gene (190 nucleotides) and from the 5'-nontranslated region (5'-ntr; 150 nu ... | 2000 | 11042397 |
| epidemiology of classical swine fever in germany in the 1990s. | in germany, 424 outbreaks of csf in domestic pigs and a great number of cases in wild boar were recorded between 1990 and 1998. most of the federal states ('bundesländer') were affected. epidemiological data from field investigations combined with genetic typing allowed to distinguish seven unrelated epidemics and a number of sporadic outbreaks in domestic pigs. detailed epidemiological data was available for 327 outbreaks. it was found that 28% of these were primary outbreaks. most of them were ... | 2000 | 11042398 |
| a serological survey on classical swine fever (csf), aujeszky's disease (ad) and porcine reproductive and respiratory syndrome (prrs) virus infections in french wild boars from 1991 to 1998. | in early 1992, a csf epizootic was clinically recognised in a wild boar population of approximately 1300 animals within an area of 250km(2) located in the east of france. in order to check the csf situation in wild boars outside this area, a serological survey was carried out in the rest of france, for 8 consecutive years (1991-1998). this paper reports on the results obtained during this survey which included wild boars shot during the hunting period but also boars reared within fences. around ... | 2000 | 11042399 |
| genetic typing of classical swine fever virus isolates from the territory of the czech republic. | epizootiological studies based on genetic typing were performed using 14 isolates from outbreaks of classical swine fever (csf) in domestic pigs and wild boar in the czech republic which occurred between 1991 and 1998. they were compared with austrian, slovakian, hungarian, polish and german isolates. the aim of this study was to characterise the csf virus isolates and find out the possible relationships between the outbreaks in domestic pigs and wild boar, and to map the spread of the virus in ... | 2000 | 11042400 |
| classical swine fever virus: a second ring test to evaluate rt-pcr detection methods. | six laboratories participated in a study to compare the sensitivity and specificity of rt-pcr tests for the detection of classical swine fever virus (csfv). sets of coded samples were prepared by serial dilution of positive samples and then distributed to each of the laboratories. one set comprised 25 samples of random primed cdna, synthesised from viral rna representative of different pestiviruses. the other set comprised samples of blood and serum obtained from virus-free or csfv-infected pigs ... | 2000 | 11042401 |
| efficacy of the classical swine fever (csf) marker vaccine porcilis pesti in pregnant sows. | the efficacy of the classical swine fever (csf) subunit marker vaccine porcilis pesti based on baculovirus expressed envelope glycoprotein e2 of csf virus (csfv) was evaluated in pregnant sows. ten gilts were vaccinated with one dose of marker vaccine, followed by a second dose 4 weeks later. four gilts remained unvaccinated and received a placebo at the same times. thirty-three days after the second vaccination all animals were artificially inseminated. neither local or systemic reactions nor a ... | 2000 | 11042402 |
| safety and efficacy of a classical swine fever subunit vaccine in pregnant sows and their offspring. | in the study three groups with five pregnant sows each were used. the animals were vaccinated twice, 2 weeks apart, in different stages of gestation, i.e. +/-4, +/-8 and +/-12 weeks after insemination and then 14 days later, respectively. from each group of sows three litters were randomly selected and vaccinated twice, 4 weeks apart, at 5 and 9, 7 and 11, and 9 and 13 weeks of life, respectively. blood for serological investigations by virus neutralisation test and elisa tests (for e(rns) antib ... | 2000 | 11042403 |
| detection of classical swine fever virus in semen of infected boars. | during the classical swine fever (csf) epidemic in 1997 in the eu member states germany, italy, spain and the netherlands, boars in an artificial insemination (ai) centre were found to be infected with csf virus. this raised a question of epidemiological importance which could not be answered immediately. can csf virus be shed by semen of infected boars and what conclusions concerning the risk of spreading csf infection by semen can be drawn. experimental studies were conducted to answer this qu ... | 2000 | 11042404 |
| the genetic basis for cytopathogenicity of pestiviruses. | two biotypes of pestiviruses, cytopathogenic (cp) and noncp viruses, can be distinguished by their effects on tissue culture cells. identification of cp bovine viral diarrhea virus (bvdv) has been frequently reported since antigenically closely related noncp and cp bvdv can be isolated from cattle with fatal mucosal disease (md) and are called a virus pair. in contrast to the bvdv system, only few cp border disease virus (bdv) and cp classical swine fever virus (csfv) strains have been described ... | 2000 | 11042405 |
| comparative immunohistopathology in pigs infected with highly virulent or less virulent strains of hog cholera virus. | eight pigs were inoculated subcutaneously with a highly virulent hog cholera virus (hcv) strain ald. the infected pigs developed severe illness and became moribund on postinoculation day (pid) 7 or pid 10. histologic lesions were characterized by severe generalized vasculitis, necrosis of lymphocytes, and encephalitis. hcv antigen was detected in crypt tonsilar epithelial cells, macrophages, and reticular endothelial cells of lymphoid tissues. antigen localization corresponded well with histolog ... | 2000 | 11055862 |
| classical swine fever: essex ban to stay in place. | 2000 | 11083043 | |
| laboratory diagnosis, epizootiology, and efficacy of marker vaccines in classical swine fever: a review. | detection of classical swine fever virus (csfv) can be achieved by a range of assays of which the most commonly used are: immunohistochemical and virus culture techniques. new developments have enabled the detection of viral proteins by enzyme-linked immunosorbent assays (elisas) and the detection of the viral genome by rt- pcr. so far, laboratory findings show that the latter assays may supplement or replace the conventional techniques in the near future. the detection of serum antibody against ... | 2000 | 11087126 |
| transmission of classical swine fever virus by artificial insemination during the 1997-1998 epidemic in the netherlands: a descriptive epidemiological study. | in the course of the 1997-1998 csf epidemic in the netherlands, two semen collection centres (scc) became infected. as an eradication strategy for an acute crisis situation, it was concluded that all semen of the boars at the sccs collected and distributed in the risk period of 28 january to 7 march 1997 was potentially contaminated (suspect semen). as a consequence, a total of 1,680 pig herds, mainly located in the southern part of the netherlands, were officially declared csf suspect. the purp ... | 2000 | 11087136 |
| deletions of structural glycoprotein e2 of classical swine fever virus strain alfort/187 resolve a linear epitope of monoclonal antibody wh303 and the minimal n-terminal domain essential for binding immunoglobulin g antibodies of a pig hyperimmune serum. | the major structural glycoprotein e2 of classical swine fever virus (csfv) is responsible for eliciting neutralizing antibodies and conferring protective immunity. the current structural model of this protein predicts its surface-exposed region at the n terminus with a short stretch of the c-terminal residues spanning the membrane envelope. in this study, the n-terminal region of 221 amino acids (aa) covering aa 690 to 910 of the csfv strain alfort/187 e2, expressed as a fusion product in escher ... | 2000 | 11090160 |