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plasmid profiles as an epidemiological marker for salmonella enterica serotype enteritidis foodborne outbreaks.the incidence of enteritidis serotype of salmonella enterica in salmonellae infections has steadily increased in spain from 27.1% in 1982 up to 63.4% in 1987. given this high incidence, we have studied the plasmid profiles of enteritidis isolates to subclassify them. different profiles were observed in 50 isolates. in 13 enteritidis serotype outbreaks, up to 5 different plasmid profiles were found. each outbreak correlated with a single plasmid profile except in one case where plasmids of two di ...19902206470
salmonella interactions with polarized human intestinal caco-2 epithelial cells.polarized monolayers of the human intestinal epithelial caco-2 cell line were grown on permeable filters and infected apically with either salmonella choleraesuis or salmonella typhimurium. both salmonella species penetrated through the monolayer, requiring 2 h before appearing in the basolateral medium. both species caused a loss in transepithelial resistance by 3-4 h, and the monolayer's integrity was completely disrupted by 6 h. scanning and transmission electron microscopy revealed that the ...19902230236
[epidemiology and clinical evaluation of salmonella enteritis].there was a local epidemic of salmonella enteritis in southern part of taiwan during the summer of 1989. from july through september 1989, a total 162 cases of enteritis were analysed in chang gung memorial hospital, kaohsiung. among them, 46 cases were proved to be salmonella enteritis by stool and/or blood culture. the identified flora group mainly group b (salmonella typhimurium, 87%), group c (salmonella choleraesuis, 6.5%) and group d (salmonella enteritidis, 6.5%). the drug resistance of s ...19902282566
nucleotide sequence of a gene encoding a 29 kda polypeptide in mba region of the virulence plasmid, pkdsc50, of salmonella choleraesuis. 19902315022
nucleotide sequences of genes encoding 32 kda and 70 kda polypeptides in mba region of the virulence plasmid, pkdsc50, of salmonella choleraesuis. 19902336400
vaccinal properties of salmonella abortusovis mutants for streptomycin: screening with an ovine model.living attenuated vaccines may help control infection with salmonella enterica subspecies enterica ser abortusovis in sheep without constraints incompatible with husbandry. virulence and immunogenicity of 2 abortusovis streptomycin-dependent strains and 3 reverse mutants were compared in sheep, the virulent parental strain abortusovis 15/5 being used as reference. reduction of virulence with conservation of some immunogenicity was demonstrated for the 5 mutant strains after intravenous or subcut ...19902344139
alternative methods of attenuating salmonella species for potential vaccine use.because of their invasive properties, salmonella species are uniquely suited for use as live oral vaccines, and mutants attenuated in general metabolic functions show promise as vaccine strains. we have identified a salmonella choleraesuis transposon mutant which is deficient in abilities to attach to, invade or penetrate through animal cells. this mutant is avirulent in mice, yet is able to persist within the liver, spleen and gut-associated lymphoid tissue for periods which may be adequate for ...19901966254
characterization of strains of salmonella enterica subsp. enterica serovar wien isolated in italy: an epidemiological evaluation.in the years 1987-1988, 110 strains of wien serovar were isolated from a gastroenteritis outbreak in a neonatal unit in palermo (sicily). these strains showed different drug resistance patterns and plasmid profiles. analysis of endonuclease restriction fragments of chromosomal dna by hybridization with e. coli rrna has demonstrated that a single bacterial clone or its derivatives were responsible for the outbreak. furthermore, the study of 139 strains, isolated since 1970 from different geograph ...19901982334
[multicenter study on bacterial gastroenteritis in regional hospitals of the province of barcelona. group of microbiologists of the regional hospitals of catalonia].a retrospective analysis of 26,901 coprocultures is reported. of these, in 5,847 (21.7%) an enteric pathogen was isolated. the positivity rate in adults was 21.0%, and 22.1% in children. statistically significant differences (p less than 0.001) were found in the number of overall isolates throughout the years for both age groups. the most common enteropathogen was salmonella enterica spp i non typhi (59.4% of the isolates in children and 76.9% in adults). the rate of isolation of campylobacter s ...19892490674
[phenolic coefficient in the microbiological evaluation of disinfectants for hospital and household use].the phenolic coefficient of 24 disinfectants (six for hospital and the remainder for household use) commercialized in s. paulo were verified. the active compounds found were phenol, quaternary ammonium, formaldehyde, ethanol and chlorine, some of them in association. the microorganisms used were staphylococcus aureus atcc 6538, pseudomonas aeruginosa atcc 15442 and salmonella choleraesuis atcc 10708. the values of the phenolic coefficient ranged from 58.3 to 0.1. the hospital disinfectants showe ...19892515584
molecular cloning and analysis of the incompatibility and partition functions of the virulence plasmid of salmonella typhimurium.the incompatibility functions (inc) of the virulence plasmid of salmonella typhimurium lt2 were initially located in a 4.3 kb region near the repa locus of the plasmid. expression of inc required the presence, in cis or in trans, of two distinct dna regions of the fragment. these regions, maximally 0.3 kb and 0.6 kb in size, were separated in the fragment by c. 3.0 kb. this intervening dna encoded two proteins, of mr values 37 kda and 40 kda. the promoter for the 37 kda protein lay in or near on ...19892556621
porcine neutrophil function in the presence of virulent and avirulent salmonella choleraesuis.porcine polymorphonuclear leukocytes (pmnls) may be activated by bacteria to begin phagocytosis followed by oxidative and non-oxidative mechanisms of killing. the purpose of this study was to identify differences between virulent and avirulent salmonella choleraesuis (s. choleraesuis) strains, 38 and 9 respectively, in their interactions with porcine pmnls using five different assays. (1) staphylococcus aureus (s. aureus) ingestion was determined by exposure of porcine pmnls to a mixture of s. c ...19892560864
vaccination route, infectivity and thioglycollate broth administration: effects on live vaccine efficacy of auxotrophic derivatives of salmonella choleraesuis.an aromatic-dependent, therefore non-virulent, derivative of a mouse-virulent strain of salmonella choleraesuis previously shown not to be effective as a live vaccine when given intraperitoneally (i.p.) to itys mice, was administered to balb/c mice. two doses given i.p. or by feeding did not protect against i.p. or oral challenge with 50 to 5000 ld50 of the virulent ancestor strain. by contrast two doses given intravenously (i.v.) gave almost complete protection against i.p. or oral challenge wi ...19892622327
prevalence of salmonella serotypes isolated in spain from human and non human sources (1983-1987).salmonella serotypes over a five year period were studied in order to know their prevalence in spain. the salmonella reference centre received a total of 17,612 strains from 1983-1987. the majority (16,133) were of human origin and only 1,479 strains were isolated from non-human sources. the serotyping yielded 100 different serotypes, salmonella enterica serotype enteritidis (8) being the commonest in both groups, 61.18% of human origin and 31.91% of non-human origin. salmonella enterica serotyp ...19892629788
long-term suppression of salmonella aortitis with an oral antibiotic.an aneurysm of the aortic arch infected with salmonella choleraesuis is presumed to be inevitably fatal unless the patient receives corrective surgery and antibiotic therapy. i report such a case in a patient who refused surgery and who has been maintained free of infectious symptoms for over 26 months using trimethoprim-sulfamethoxazole administered orally.19892786398
in vitro activity of tigemonam against urinary tract pathogens.tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serratia marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory ...19892809699
clonal nature of salmonella typhi and its genetic relatedness to other salmonellae as shown by multilocus enzyme electrophoresis, and proposal of salmonella bongori comb. nov.crude cell extracts of 26 isolates of salmonella serotype typhi (s. typhi) and 48 other salmonella isolates representing 28 serotypes and seven dna hybridization subgroups were analyzed for electrophoretic variants of 24 metabolic enzymes by starch gel electrophoresis. all strains of s. typhi had identical isoenzyme patterns, indicating that they were a single clone. all of the enzymes detected in the remaining strains were polymorphic, and the degree of genetic variation was quite high. the ave ...19892915026
in vitro activity of tigemonam against urinary tract pathogens.tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serrada marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory c ...198927416146
bacterial numbers on penicylinders used in disinfectant testing: use of 24 hour adjusted broth cultures.the current aoac use-dilution methods of disinfectant efficacy testing require the use of 48-54 h unadjusted broth cultures of salmonella choleraesuis, staphylococcus aureus, and pseudomonas aeruginosa for the inoculation of stainless steel penicylinders. the use of unadjusted broth cultures contributes to noncomparable numbers of organisms on penicylinders among the test strains due to relative efficacy of bacterial attachment to penicylinders and to bacterial numbers in broth. to achieve compa ...19883129396
comparison of the invasion strategies used by salmonella cholerae-suis, shigella flexneri and yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.strains of escherichia, salmonella, shigella and yersinia actively enter eukaryotic cells. several techniques were used to compare and contrast the invasion mechanisms of salmonella cholerae-suis, yersinia enterocolitica and shigella flexneri. three animal cell lines (cho, hep-2 and mdck) were examined for susceptibility to bacterial entry by these strains. levels of intracellular bacteria varied widely between cell lines, but cho cells were the most susceptible to bacterial invasion, hep-2 inva ...19883147700
quantitative evaluation of bacteria washed from stainless steel penicylinders during aoac use-dilution method.stainless steel penicylinders inoculated separately with test bacteria (salmonella choleraesuis, pseudomonas aeruginosa, or staphylococcus aureus) are used in the aoac use-dilution method (udm) for disinfectant efficacy testing. numbers of bacteria remaining on penicylinders were quantitatively assessed to determine if cells are washed from the penicylinders after a 10 min exposure to phosphate buffer dilution water (pbdw). inoculated penicylinders were also examined by scanning electron microsc ...19883148609
disinfectant testing using a modified use-dilution method: collaborative study.an initial collaborative study of the aoac use-dilution method (udm), used for bactericidal disinfectant efficacy testing, demonstrated extreme variability of test results among the 18 laboratories testing identical hospital disinfectants. in an effort to improve the method, 32 changes were made by the udm task force. these changes represented improvements in quality assurance practices and elimination of method variability; however, the basic framework of the method was retained. a second colla ...19883149273
occurrence of h-antigen z66 of r phase in cultures of salmonella serovar typhi originated from indonesia.eighteen strains of salmonella choleraesuis subsp. choleraesuis serovar typhi (s. typhi) isolated from blood of patients in japan who had visited indonesia and returned before the onset of typhoid fever were found to possess the h-antigen z66 reported by guinée et al. (1981) but had the naturally occurring h-antigen d. they were not lysed by any of the phages of the vi phage typing system. after passage through semi-solid medium containing h-z66 antiserum, h-antigens of 11 of 18 cultures with h- ...19883181315
quantitative analysis and partial characterization of cytotoxin production by salmonella strains.the pathogenesis of the wide-spectrum human disease caused by salmonella species is poorly understood. cytotoxin production by other enteric pathogens has been increasingly investigated recently, and data are accumulating regarding the role of cytotoxins in enteric infections and hemolytic uremic syndrome. we studied the cytotoxic activity of 131 salmonella strains of the major serotypes, including 94 strains of salmonella enteritidis, 12 strains of salmonella typhi, and 25 strains of salmonella ...19883182072
evidence of correlation between 50-kilobase plasmid of salmonella choleraesuis and its virulence.a plasmid of 50 kilobases (kb) was found in all six strains of salmonella choleraesuis studied, originally isolated from septicemic swine or man. a derivative cured of the 50 kb plasmid was isolated after treatment with novobiocin, and its virulence compared to that of the parent by intraperitoneal injection into mice. the lethality of the parent strain was found higher than that of the cured strain. with a sublethal number of cells of the parent strain injected, the spleen, liver and lymph node ...19883200162
clinical safety and efficacy of ofloxacin.in this study we report about the efficacy and tolerability of ofloxacin in the treatment of 15 patients with severe and moderately severe infections including osteomyelitis (5), soft tissue infections (5), salmonellosis in aids patients (2), acute or chronic pulmonary infections (2) and mediastinitis (1). the following organisms were isolated in culture specimens: staphylococcus aureus (4), pseudomonas aeruginosa (4), staphylococcus epidermidis (3), serratia marcescens (1), escherichia coli (1) ...19883224401
abscess thyroid due to salmonella cholerae-suis--a rare presentation of salmonellosis. 19883229806
[the aerobic intestinal flora of the microchiropteran bat chaerephon pumila in madagascar].from stools of 88 malagasy insect eater bats chaerephon pumila, a large amount of bacterial strains were isolated. twenty different species were recognized, most of them belonged to enterobacteriaceae family. eight strains were identified as salmonella enterica subsp. enterica o48: -; 1.5, despite many atypical characters. strains of salmonella enteritidis, koserella trabulsii, kluyvera sp., odc negative serratia marcescens, atypical hafnia alvei... were also identified.19883240566
[acute bacterial sacroiliitis caused by salmonella cholerae-suis].a 17-year-old schoolboy was admitted to hospital because of one-sided pelvic pain of uncertain aetiology and fever gradually rising over several days. bacteriological analysis of blood cultures, skeletal scintigraphy and computed tomography revealed sacroiliitis caused by salmonella cholerae-suis. specific antibiotic treatment quickly stopped all symptoms and cured the infection. radiologically there remained sclerosis of the sacro-iliac joint.19883262497
penetration of salmonella through a polarized madin-darby canine kidney epithelial cell monolayer.many intracellular parasites are capable of penetrating host epithelial barriers. to study this process in more detail we examined the interactions between the pathogenic bacteria salmonella choleraesuis and polarized epithelial monolayers of madin-darby canine kidney (mdck) cells grown on membrane filters. association of bacteria with the mdck cell apical surface was an active event, requiring bacterial rna and protein synthesis, and was blocked by low temperatures. salmonella were internalized ...19883292541
extraintestinal salmonellosis.between 1969 and 1984, 6564 non-typhoid salmonella strains were isolated at the liverpool public health laboratory of which 194 (3.0%) were from extraintestinal sites. blood (34%) and urine (32%) isolates accounted for two-thirds of these, with the remainder being recovered from pus and inflammatory tissue (23%), bone (5%), cerebrospinal fluid (5%) and sputum (3%). certain serotypes tended to cause more invasive disease than others, i.e. salmonella choleraesuis, s. dublin, s. london, s. virchow ...19883378582
multiple typing of strains of salmonella enterica subsp. bongori ser. 48:z35:- isolated in southern italy.in 1984-87, 10 isolates of salmonella enterica subsp. bongori ser. 48:z35:-, 9 of human source, were identified at the southern italy centre of enterobacteriaceae. this serotype had never been identified in southern italy before 1984. the combined use of different typing methods, with particular reference to restriction enzyme fingerprinting of plasmid and chromosomal dna, supports the hypothesis that all bongor serovars derive from a single strain.19882855307
[effect of components of curing mixtures on the antagonistic properties of enterococci and other bacteria isolated from cured meat].effect of sodium chloride, glucose, sodium glutamate, potassium nitrate, sodium nitrate, sodium nitraite, ascorbic acid, sodium citrate, polyphosphates (hamine) on antagonistic activity of enterococci and various bacteria isolated from cured meat against bacillus cereus, bacillus subtilis, salmonella typhi-murium, salmonella cholerae-suis, staphylococcus aureus, clostridium botulinum type a, clostridium botulinum type b, clostridium botulinum type e, clostridium bifermentans, clostridium perfrin ...19873506173
the effects of o-antigen character and enterobacterial common antigen content on the in vivo persistence of aromatic-dependent salmonella sp. live-vaccine strains.aromatic-dependent (aro) derivatives of salmonella choleraesuis like aro s. typhimurium are non-virulent but, unlike them, are ineffective as live vaccines in mice, given i.p. an aro derivative of s. choleraesuis did not persist in the liver and spleen (res) of mice after i.p. inoculation whereas a similar derivative of s. typhimurium persisted. s. choleraesuis (o group c1; o-6,7) and s. typhimurium [o group b; o-(1),4(5),12] differ in o antigen of lps, determined by chromosomal locus, rfb. thre ...19872461504
[orchitis and epididymitis caused by salmonella cholerae suis in a premature infant].it is reported on a premature infant who fell ill with an orchitis and epididymitis by salmonella cholerae suis in the fourth week of life. the proof of the germs was carried out by means of two blood cultures as well as in the smear from the periorchium. the most important informations from literature concerning this subject are briefly reviewed.19873591031
conjunctival and intramuscular vaccination of pigs with a live avirulent strain of salmonella cholerae-suis.an avirulent mutant strain of salmonella cholerae-suis was cloned for resistance to streptomycin and nalidixic acid. the mutant strain 33-13 also was used because of its avirulence and immunogenicity in mice. weaned pigs were vaccinated with live strain 33-13; 5 pigs were vaccinated by conjunctivally administered 5.5 x 10(7) organisms (low dose), 5 were conjunctivally administered 5.5 x 10(9) organisms (high dose), and 5 pigs were administered 5.5 x 10(9) organisms (high dose) im. transient feve ...19873631689
[antibacterial activity of chlorquinaldol esters and 2-styryl-substituted derivatives].studied was the bacteriostatic activity of a total of 17 new esters and replaced 2-styryl derivatives of chlorquinaldol. determined were also the lowest concentrations that suppressed the growth of organisms. some of the compounds showed higher activity and broader spectrum of antibacterial qualities, mainly against escherichia coli, salmonella gallinarum, and salmonella cholerae suis as compared to the initial therapeutic preparation chlorquinaldol. it was found that the presence of chlorine at ...19873672924
effects of infection with enterobacteriaceae enteropathogens on subsequent infection with ascaris suum in the laboratory mouse.prior to infection with ascaris suum, icr strain mice were inoculated with salmonella typhisuis intraperitoneally or via gastric gavage. similarly, salmonella cholerae-suis var. kunzendorf, salmonella typhimurium and enterotoxigenic escherichia coli were administered to mice via gastric gavage 2 weeks prior to a. suum inoculation. previous inoculation with s. typhisuis, via the intraperitoneal or gastric gavage routes and s. cholerae-suis var. kunzendorf decreased recovery of ascarid larvae from ...19873296422
standardization of bacterial numbers of penicylinders used in disinfectant testing: interlaboratory study.an interlaboratory study was conducted to evaluate a method of standardizing bacterial numbers on penicylinders used in the aoac use-dilution method (4.007-4.015) of disinfectant testing. eight participating laboratories followed a broth adjustment method using their media and stock cultures of staphylococcus aureus atcc 6538, salmonella choleraesuis atcc 10708, and pseudomonas aeruginosa atcc 15442. the culture broths that were used to inoculate the penicylinders were incubated for 48 h at 37 d ...19873305474
test of the virulence and live-vaccine efficacy of auxotrophic and gale derivatives of salmonella choleraesuis.aromatic compound-dependent (aro) derivatives of three mouse-virulent strains of salmonella choleraesuis (salmonella cholerae-suis) were constructed and shown to be nonvirulent for mice (intraperitoneal [i.p.] 50% lethal dose [ld50], greater than 5 x 10(6) cfu). a pur derivative, and a thy derivative, each of a different virulent parent, remained moderately virulent (i.p. ld50s for balb/c mice, ca. 10(5) and 5 x 10(4) cfu, respectively). tested as live vaccines i.p., the aro strains were ineffec ...19873104209
evaluation of penicylinders used in disinfectant testing: bacterial attachment and surface texture.two possible deficiencies in the aoac use-dilution method for registration of chemical disinfectants by the environmental protection agency are examined: (1) the physical disparities among brands of penicylinders and (2) the variability of bacterial numbers on penicylinders depending upon test strain and penicylinder surface texture. textural differences of 2 brands of stainless steel penicylinders, one brand of porcelain, and one brand of glass were assessed by scanning electron microscopy. a c ...19873119561
ineffectiveness of hospital disinfectants against bacteria: a collaborative study.a collaborative study was undertaken to assess the degree of variability in disinfectant efficacy test results among laboratories that routinely perform the association of official analytical chemists (aoac) use-dilution method. eighteen laboratories tested identical samples of six epa-registered, hospital-grade disinfectants (three phenolics and three quaternaries) at the manufacturers' recommended use-dilution using only those modifications of the method approved by the aoac use-dilution task ...19873123412
virulence-associated plasmids of salmonella serotype typhimurium in experimental murine infection.the growth pattern of salmonella enterica subsp. enterica serotype typhimurium (typhimurium) was studied in mice to examine the role of the 60-mdal virulence-associated plasmid in the pathogenesis of mouse typhoid. after repeated subcultures at 45 degrees c, isogenic variants harbouring the virulence-associated plasmid (strains c52, tm122 and tm332) or having lost this large plasmid (strains c53, tm123 and tm333) were obtained from three parental strains (strains c5, tm12 and tm33, respectively) ...19863324943
salt-induced filamentous growth of a salmonella strain isolated from blood.a strain of salmonella choleraesuis subsp. choleraesuis serovar paratyphi-a isolated from the blood of a febrile patient grew into filaments on a nutrient agar containing various salts, such as nacl, kcl, mgcl2, nh4cl, (nh4)2so4, or (nh4)2hpo4, at concentrations of 50 to 400 mm. the filamentous cells were nonseptate and multinucleate, and they had colony-forming ability. this mutant strain, however, did not show filamentous growth in liquid media which contained the same salts. on nutrient agar ...19863700602
desferoxamine and iron dextran in acute salmonella cholerae-suis infection in pigs.serum iron (si)-related and hematologic changes were evaluated in a herd of weaned pigs inoculated with a strain of salmonella cholerae-suis, causing 83% mortality within 22 days after inoculation was done. serum iron concentrations decreased to 35% of base-line values 2 days after inoculation was done, but recovered to near base line subsequently. total si-binding capacity (tibc) decreased gradually for 14 days after inoculation was done. transferrin (tf) concentrations decreased to near half t ...19863740614
aztreonam therapy for serious gram-negative infections in children.fifty-nine children were enrolled in an open trial of aztreonam, a monocyclic beta-lactam, therapy for serious gram-negative infections. thirty-six infections were microbiologically evaluable and received five or more days of therapy. patients' ages ranged from 3 days to 12 years, and diagnoses included pyelonephritis or cystitis (20), deep soft tissue or joint infection (seven), septicemia (four), pneumonia (three), peritonitis, and epiglottitis. causative bacteria included escherichia coli and ...19863766490
some gale mutants of salmonella choleraesuis retain virulence.gale mutants were isolated from three mouse-virulent strains of salmonella choleraesuis (of group c1, o antigen 6,7) by selection for resistance to 2-deoxygalactose. the gale derivative of strain 381 comprised two components: galactose sensitive, thought to be the original mutant; and galactose resistant, presumably by a second mutation reducing galk or galt function or both. the galactose-sensitive component had an intraperitoneal 50% lethal dose for balb/c mice of ca. 4 x 10(6) cfu, whereas th ...19863781619
[salmonella choleraesuis bacteremia. apropos of a case]. 19863784664
antibiotic susceptibilities of swine salmonella isolants from 1979 to 1983.two hundred seventy-seven salmonella (27 serotypes) were isolated from kansas swine over a 5-year period (1979 to 1983). salmonella cholerae-suis was the predominant isolant from all tissues and made up 94.4% of the salmonella isolants from the lungs. the percentage of salmonella isolants susceptible to various antimicrobial agents was determined. the percentage of salmonella susceptible to carbadox decreased from 1980 to 1983.19863789494
salmonella bacteremia as manifestation of acquired immunodeficiency syndrome.multiple opportunistic infections are characteristic of the acquired immunodeficiency syndrome (aids). although bacterial pathogens have presented few problems, we have noted an emerging problem with salmonellal infection among patients with aids. a review of all stool and blood cultures from adults between january 1982 and july 1984 showed that 80 stool cultures were positive for salmonella species; serogroup b was the most common isolated. eight (10%) were isolated from patients with aids. nin ...19863942443
studies on the pathogenesis of salmonella typhimurium and salmonella choleraesuis var kunzendorf infection in weanling pigs.twenty-six 4-week-old pigs were randomly allotted to 4 groups: group 1--orally inoculated with salmonella typhimurium; group 2--orally dosed with s choleraesuis; and groups 3 and 4, with surgically constructed intestinal loops--loops inoculated with either s typhimurium or s choleraesuis. one pig each from groups 1 and 2 was killed at 8, 12, 24, 48, 72, 96, and 120 hours after inoculation. one pig each from groups 3 and 4 was killed at 2, 4, 6, 8, 12, and 24 hours after intestinal loop inoculati ...19863511804
[microcalorimetric study of salmonella].we investigated the heat production of 42 different salmonella strains belonging to the serogroups a, b, c, d and e. using columbia- and brain heart infusion broth as growth media, we found 3 different types of heat profiles. salmonella typhi and salmonella choleraesuis showed thermograms which differed in shape from the remaining serotypes. the thermograms of one salmonella dublin isolate which showed an atypical biochemical behaviour could not be put into proper relation to one of these 3 micr ...19863541456
bacteriophages mediating somatic antigenic conversion in salmonella cholerae-suis: their isolation from sewage and other salmonella serotypes possessing the somatic 6 antigen.bacteriophages which mediate the conversion of the o somatic antigen of salmonella cholerae-suis from the 6(2)7 to the 6(1)7 phenotype have been isolated from two strains of s. newport and one of s. muenchen, and also from sewage collected from two areas where there have been no reports of s. cholerae-suis infection for several years. the phages differed from each other by cross-resistance tests.19862426391
iron and transferrin in acute experimental salmonella cholerae-suis infection in pigs.the effects of experimental salmonella cholerae-suis inoculation with a virulent and an avirulent strain on serum iron (si), total iron-binding capacity (tibc), and transferrin (tf) were evaluated. inoculation of virulent strain 38 was followed by significant (p less than 0.05) decreases of si, tibc, and tf. exposure to avirulent strain 33 was followed by moderate decreases of si, tibc, and tf. when exposure to avirulent strain 33 was followed by challenge exposure with virulent strain 38, the s ...19853994112
[effect of levamisole on white mice and on the immunological reactivity of guinea pigs].investigations were carried out on the protective action of therapeutic doses of levamisole (pharmachim) on mice with a ld100 salmonella gallinarum infection. studied was also the effect of levamisole on the immunologic response of guinea pigs infected with salmonella cholerae suis. it was found that therapeutic amounts of 3 mg/kg levamisole applied to mice following respective patterns protected them against a lethal dose of s. gallinarum. the animals showed enhanced resistance to the infection ...19854035985
salmonella osteomyelitis. an important differential diagnosis of granulomatous osteomyelitis.a case of septicemia owing to salmonella choleraesuis with localization in the lumbar spine and left knee is described. the spinal lesion is dominated by tuberculoid granulomas with or without central necrosis. the necrotic foci within some granulomas show heavy polymorph infiltration, whereas in others they simulate caseous necrosis and are indistinguishable histologically from tuberculosis, brucellosis, and fungal infections. as salmonella osteomyelitis has a strong tendency to chronicity if a ...19854091185
salmonella choleraesuis endocarditis. 19846589489
[pleural empyema caused by salmonella cholerae suis in a patient with breast cancer in the stage of metastatic dissemination].a pleural localisation of salmonella cholerae suis infection is described in a patient with breast carcinoma. the evolution of neoplastic disease seems to be more slow after salmonellosis. on the basis of this case the most important mechanisms between immunity and tumors are discussed.19846728272
effects of cyclophosphamide on the immune response of pigs to salmonella cholerae-suis var kunzendorf.the effects of the immunosuppressive agent cyclophosphamide (cy) on the immune response of pigs given im challenge inoculations of a moderately virulent strain of salmonella cholerae-suis var kunzendorf were examined. five groups of yorkshire-cross pigs (approx 6 kg) were given salmonella, cy, or both at various times after the 1st of a series of doses of cy was given. the drug was administered subcutaneously in 3 doses, 2 days between doses, at a rate of 20 mg/kg of body weight. cyclophosphamid ...198424049894
relationship between the antibody-complement susceptibility of smooth salmonella cholerae-suis var kunzendorf strains and their virulence for mice and pigs.in the present work, we attempted to characterize 4 field strains of salmonella cholerae-suis var kunzendorf susceptible to antibody and complement (ab-c), and 4 strains not susceptible to ab-c, with respect to their virulence for mice and pigs. in vivo growth of an ab-c-susceptible, mouse-virulent strain in the spleens of mice exceeded that of 2 ab-c-susceptible, but mouse-avirulent strains by at least 10(4) organisms. comparison of ab-c susceptibility with virulence for pigs seemed to indicate ...198424049895
sensitivity of smooth salmonella cholerae-suis var kunzendorf to killing by porcine polymorphonuclear neutrophils and its relation to mouse virulence.twenty-two selected strains of salmonella cholerae-suis var kunzendorf were evaluated for their in vitro susceptibility to the actions of porcine peripheral blood polymorphonuclear neutrophils (pmns) and/or antibody (ab). strains were divided into 2 groups based on their previously reported sensitivity or resistance to antibody-complement (ab-c) lysis. strains resistant to ab-c lysis resisted killing by pmns to a greater extent than strains susceptible to ab-c lysis. in the absence of pmns, dilu ...19836612987
mycotic aortic aneurysm. a complication of campylobacter fetus septicemia.the first surviving case, to our knowledge, of a campylobacter fetus mycotic aortic aneurysm is reported. bacteremia and an ileofemoral thrombophlebitis preceded the development of the infected aneurysm, reconfirming the vascular tropism of this organism. the clinical similarity with infections caused by salmonella choleraesuis is illustrated by this case. the full recovery of our patient attests to the efficacy of extralanatomic bypass combined with long-term antibiotic therapy in the treatment ...19836687525
salmonella infections of the abdominal aorta.endovascular infection of the atherosclerotic aorta is a substantial risk in patients older than 50 years of age who have bacteremia due to salmonella. until recently, the resultant salmonella aortitis was uniformly fatal. two patients who were cured are described, and the characteristics of 10 other reported long-term survivors are given. this recent improvement in prognosis may have been related to (1) earlier diagnosis, (2) improved surgical management (axillofemoral bypass graft), and/or (3) ...19836844803
pneumonia due to salmonella choleraesuis in infants in papua new guinea. 19836136739
sensitivity of smooth salmonella cholerae-suis var kunzendorf field strains to antibody complement under various conditions.seventy-two field strains of smooth salmonella cholerae-suis var kunzendorf were obtained from outbreaks of swine parathyroid. the field strains were examined for susceptibility to killing by antibody-complement (ab-c) after they were exposed to tris-edta, lysozyme, or saline solution. considerable differences were found in susceptibility to killing by ab-c in the strain population. some strains were highly sensitive to ab-c under all experimental conditions, whereas others became sensitive only ...19826808877
[advantages of using semi-solid agar medium for the isolation of salmonellae].a method of salmonellae rapid isolation on semi-solid agar medium in glass u-shaped tubes was tested and the results were compared with those obtained by using the selective multiplication medium after rappaport and kauffmann medium with sub-cultures on agars with brilliant green and sodium desoxycholate. this method was verified by employing the collection strains of selected serotypes of salmonellae, e. coli collection strains and microorganisms of the genus proteus. the described method was u ...19826812264
pyridoquinoxaline n-oxides. 2. synthesis and antibacterial activity of tricyclic lactams.a series of novel 3,4-dihydropyrido[3,4-b]quinoxalin-1(2h)-one 5,10-dioxides was synthesized using an intramolecular amidation reaction. the lactams were screened in vitro and in vivo against salmonella choleraesuis, pasteurella multocida, and escherichia coli. an n-methyl analogue was the most potent member of this series, with antibacterial activity comparable to that of the commercially important quinoxaline 1,4-dioxide carbadox.19827108904
[various biochemical properties of salmonella strains isolated from animal food products].studied were a total of 488 salmonella strains isolated from food products and washings from productional surfaces for the presence of variants, with the use of tests of producing gas from glucose and hydrogen sulfide and the coincidence of the two properties. it was found that 7 species only had biochemical variants. most frequent were the variants with s. cholerae suis. biochemical variants were found in 24.79 per cent of the typed salmonellae, these of the s. cholerae suis species being 19.26 ...19827135819
salmonella thyroiditis, apathetic thyrotoxicosis, and follicular carcinoma in a laotian woman.a 49-year-old laotian woman presented with apathetic hyperthyroidism and suppurative thyroiditis from salmonella cholerae-suis occurring in a multinodular gland with a large right-sided mass. apathetic hyperthyroidism in this age group is uncommon and salmonella thyroiditis has been infrequently described. surgical removal of the mass which had effectively concentrated radioactive iodine cured the hyperthyroidism and permitted the remaining suppressed normal tissue to regain function. pathologic ...19827139541
porcine proliferative enteritis: experimentally induced disease in cesarean-derived colostrum-deprived pigs.the hypotheses that porcine proliferative enteritis is an infectious disease and that campylobacter sputorum subsp mucosalis (csm) is involved in the development of this disease were experimentally tested. three experiments were conducted with 10-week-old, cesarean-derived colostrum-deprived pigs. of 22 pigs given homogenized mucosal scrapings (crude inocula) intragastrically, 15 had gross and/or microscopic lesions of proliferative enteritis. of 10 pigs inoculated with cultures of both csm and ...19827149408
[salmonella cholerae-suis infections]. 19827162804
observations on the occurrence of salmonella cholerae-suis and other salmonellas in two herds of feeder pigs.modified brilliant green agar (bga), muller-kauffmann tetrathionate, rappaport's and selenite f broths were compared for their efficiency in isolating salmonellas from pigs and their excreta. it was concluded that bga and rappaport's broth were the media of choice. where searches were made for salmonella cholerae-suis alone, the use of a trehalose mcconkey agar provided a rapid method of differentiating s. cholerae-suis, which does not ferment trehalose, from the majority of other salmonellas, w ...19817240737
cephamycin c treatment of induced swine salmonellosis.weanling pigs in groups of 12 were infected orally with salmonella choleraesuis and were treated intramuscularly with doses of cephamycin c ranging from 12.5 to 337.5 mg twice daily for 10 days beginning 1 day postinoculation. pigs in two other infected groups either received 300 mg of tetracycline orally on a similar schedule or served as nonmedicated controls. optimal responses to cephamycin c were achieved at a twice daily dose of 112.5 mg. with this regimen, the febrile response was signific ...19817247378
pig neutrophil adherence in experimentally induced salmonellosis.in vitro neutrophil adherence was characterized in healthy newly weaned pigs following experimentally induced infection with salmonella choleraesuis, a typhimurium, and s anatum. the adherence of pig neutrophils to nylon fiber columns was shown to be reproducible on a day-to-day basis. neutrophil adherence was significantly increased in a group of pigs at 6, 30, and 48 hours after s choleraesuis was inoculated (orally). neutrophil adherence was not significantly altered in groups of pigs inocula ...19817271046
[inflammation of the hip joint and femur head caused by salmonella cholerae suis]. 19817313138
[reproduction of salmonellosis in swine by aerosol infection].four 36-day-old pigs were infected through aerosol with salmonella cholerae suis var. america, and one pig was injected with same pathogen intravenously as a control, all animals being treated in a chamber. the temperature and the other clinical signs were followed up. rectal samples and hemocultures were investigated for salmonella organisms. on the 4th, 8h, 14th, and 25th day a pig was killed for bacteriologic and histopathologic investigations of the viscera. as early as the 48th hour there w ...19817324376
salmonella choleraesuis septicemia in calf. 19817340933
introduction and reisolation of selected gram-negative bacteria from fermented edible wastes.a lactobacillus fermentation process, using edible food wastes, was tested for its ability to eliminate selected bacterial pathogens. this fermentation process converts food wastes into a feed ingredient for animal consumption. six gram-negative bacterial pathogens of potential zoonotic importance were tested. these experimental organisms were: salmonella enteritidis serovar typhimurium, s enteritidis serovar anatum, s cholerae-suis, yersinia enterocolitica, y pseudotuberculosis, and pasteurella ...19816794391
pneumonia of pigs: a review.pneumonia of pigs is one of the more important disease factors limiting pig production. of the varieties of pneumonia affecting this species enzootic pneumonia caused by mycoplasma spp. is the most common and most important. the major effects of this disease are lowered food conversion ratio and poor weight gain. deaths are usually the result of secondary infection by necrotising, pus-forming bacteria. eradication of the disease is expensive and requires depopulation and restocking. control and ...198116030803
salmonella choleraesuis encephalopathy. 19806932598
antibacterial antibodies in swine colostrum.antibacterial antibody activities were examined in 138 samples of swine colostrum by o-agglutinin test with serotypes of escherichia coli (o-6, 0-45, 0-81, 0-83, 0-115, 0-138, o-139, and o-126), salmonella typhimurium, and salmonella cholerae suis. the mean agglutinin titer of e coli o-83 was the highest; e coli o-115 was next highest. the mean agglutinin titer of s cholerae suis was lowest; s typhimurium was next to lowest. correlation coefficients among these o-agglutinin titers were between 0 ...19806989301
the antigenic relationship between candida and salmonella o:6(2), o:7 antigens.the immune sera for candida albicans, c. guilliermondii and c. tropicalis agglutinated salmonella cholerae-suis (o:6(2),7). the immune serum for s. cholerae-suis agglutinated c. albicans, c. guilliermondii and c. tropicalis. absorption and agglutination cross tests demonstrated common antigenic factor between above mentioned yeasts and salmonella o:6(2), o:7 antigens.19806158816
the o antigenic relationships amongst dekkera, hanseniaspora, escherichia coli o:75, salmonella cholerae-suis and salmonella aberdeen.the immune sera for dekkera bruxellensis, d. intermedia and hanseniaspora osmophila agglutinated salmonella cholerae-suis (o:62,7) and vice versa. the immune serum for h. osmophila also agglutinated e. coli (o:75), s. aberdeen (o:11) and vice versa. cross agglutination and absorption tests demonstrated common antigenic factor(s) between above mentioned yeasts and salmonella o:62, o:7 antigens. h. osmophila also showed a common antigenic factor(s) with e. coli (o:75) and s. aberdeen (o:11).19806162296
increased susceptibility of lead-exposed swine to salmonella choleraesuis var kunzendorf.the effect of oral subclinical lead exposure on the susceptibility of swine to salmonella choleraesuis var kunzendorf was studied in two experiments. in experiment 1, 18 pigs were exposed to lead acetate and 18 pigs were exposed to sodium acetate for 1, 2, or 3 weeks and then challenge exposed intraperitoneally. in experiment 2, 8 pigs were exposed to lead chloride for 2 weeks, 8 pigs were exposed to lead chloride for 1 week, and 8 pigs were exposed to sodium chloride for 2 weeks. these pigs wer ...19807406264
[suisaloral "dessau"--a salmonella cholerae suis live vaccine for oral, parenteral and combined applications].an account is given of all immune preparations which had been available by summer 1976 and their uses in public disease control, with reference being made to the epizootiological role and detrimental effects of salmonella cholerae suis (s. c.-s.). the need for using live salmonella strains to test their immunogenic properties is derived from a thorough assessment of inactivated s.-c.-s.-vaccine, salmoporc "dessau", which had been used in the past. salmonellae are pathogens of zoo-anthroponoses b ...19807416899
[diagnosis of some yeasts in metschnikowia genus with the aid of salmonella cholerae-suis o agglutinating serum (author's transl)].in this paper a common antigenic factor among salmonella choleraesuis 0 antigen and standard metschnikowia bicuspidata var. bicuspidata and m. pulcherrima strains is shown. this common factor was not present in m. bicuspidata var. australis, m. bicuspidata var. california, m. krissii and m. reukaufii strains. m. bicuspidata var. chathamia and m. zobellii showed agglutination in the previous experiments. according to these results, the use of s. choleraesuis 0:6,7 agglutinating serum for slide an ...19807464579
[the antigenic relationship between kloeckera and salmonella cholerae-suis o antigen (author's transl)].the immune sera for kloeckera africana, k. corticis, k. javanica var. javanica and k. javanica var. lafarir agglutinated salmonella cholerae-suis (o:6(2),7). the immune serum for s. cholerae-suis agglutinated k. africana, k. corticis, k. javanica var. javanica and k. javanica var. lafarii. absorption and agglutination cross tests demonstrated common antigenic factor(s) between above mentioned yeasts and salmonella o:7 antigen and also k. javanica var. lafarii and salmonella o antigens 6 and 7.197994725
salmonella choleraesuis proteins and their relation to proteins from bacteria of heterologous sero-groups.a diversity of proteins was identified in the material isolated from s. choleraesuis with the help of sera prepared in rabbits with this material. the sera displayed, in agar-gel diffusions, numerous superimposed precipitation lines against proteins from: salmonellae, shigellae and e. coli. in contrast to proteins from s. paratyphi c, sharing identical identical 'o' 'factors, the serological activity of the s. choleraesuis proteins was impaired by heating. the immunochemical analysis of the sera ...1979120926
[antigenic relationships between salmonella choleraesuis strains and torulopsis (author's transl)].antigenic relationships between candida clausseni and salmonella choleraesuis were presented before. in this study, antigenic relationships between salmonella choleraesuis and different strains of torulopsis analyzed by immunodiffusion studies. antigenic relationships between intragroups of torulopsis were also studied. the results showed that the common thermostable component (s) of s. choleraesuis and torulopsis musae may probably be related to the common enterobacterial antigen. other results ...1979122004
evaluation of a live salmonella choleraesuis vaccine by intranasal challenge.weaned pigs were immunised with a live attenuated salmonella choleraesuis vaccine. the protective immunity induced was compared with that of unvaccinated pigs by intranasal challenge with a field strain of s choleraesuis. of 18 unvaccinated pigs, three died as a result of challenge from s choleraesuis and 10 of the survivors developed chronic lung lesions. none of the vaccinated pigs died as a result of challenge and two only showed macroscopic evidence of salmonellosis on autopsy three weeks af ...1979262604
experimental klebsiella and salmonella infection in neonatal swine.twenty colostrum-fed piglets from three sows were separated from the sows 24 hours after birth and were randomly divided into five groups of four piglets each. every piglet in each of four test groups was orally inoculated with about 10(10) colony forming units of salmonella typhimurium, salmonella choleraesuis var kunzendorf or one of two isolates of klebsiella pneumoniae. one group served as uninoculated controls. piglets infected with k. pneumoniae developed severe diarrhea beginning about 12 ...1979387185
[the antigenic relationships between candida albicans, salmonella cholerae suis, and salmonella cholerae suis var. kunzendorf o antigens].in this study a common antigenic factor (s) between c. albicans cbs-562 and s. cholerae suis 211 (0:6(2),7) was shown with agglutination and absorption tests. this common antigenic factor (s) is different than previously shown salmonella 0:7 antigen. this new factor (s) is not present in s. cholerae suis var. kunzendorf 1350 (0:6(1), 7) and s. cholerae suis var. kunzendorf 5210 (0:6(2),7) serotypes.1979400300
synthesis and antibacterial activity of 1-hydroxy-1-methyl-1,3-dihydrofuro[3,4-b]quinoxaline 4,9-dioxide and related compounds.a free-wilson analysis of the antibacterial activity found in a variety of quinoxaline 1,4-dioxides prepared and tested in these laboratories unexpectedly predicted that potent activity should be found in the case where the heterocyclic ring system was substituted with an acetyl group in the 2 position and a hydroxymethyl group in the 3 position (2). the synthesis and antibacterial activity of this compound, which was actually isolated in the hemiketal form (3), and of several of its derivatives ...1979490559
the antigenic relationship between brettanomyces-debaryomyces strains and the salmonella cholerae-suis o antigen.the immune sera for brettanomyces lambicus, b. claussenii, debaryomyces hansenii and d. marama agglutinated salmonella cholerae-suis (0:6(2), 7). the immune serum for s. cholerae-suis agglutinated b. lambicus, b. clausenni, d. hansenii and d. marama. absorption and agglutination cross-tested demonstrated common antigen factor(s) in the tested yeasts and salmonella 0:7 antigen.1978567026
bacteriophage specificity in the identification of yersinia pestis as compared with other enterobacteria.bacteriophage typing of yersinia pestis and the specificity of the phage among enterobacteriaceae were investigated. the bacteriophage used for rapid identification of y. pestis reacted with representative strains of all recognized species of shigella as well as with salmonella cholerae-suis. reactive shigella serotypes were sh. dysenteriae 1 and 9, sh. flexneri 2a, sh. boydii 1 and 6, and sh. sonnei. patterns consisting of isolated plaques (two cases) or absence of plaques were observed when th ...1978375327
synthesis and antibacterial activity of isomeric 6- and 7-acetyl-3-methyl-2- quinoxalinecarbozamide 1,4-dioxides.the synthesis, separation, and structure determination of 6- and 7-acetyl--3-methyl-2-quinoxalinecarboxamide 1,4-dioxides are reported together with a comparison of their antibacterial activity. the structural assignment of these 6- and7-acetyl isomers was based on nmr analysis of related mono-n-oxide derivatives, which were obtained by treatment of the quinoxaline 1,4-dioxides with acetic anhydride--acetic acid or trimethyl phosphite. the compounds were screened for in vitro and in vivo activit ...1978660596
salmonella arteritis: a precursor of aortic rupture and pseudoaneurysm formation.salmonella arteritis developed in three patients with subsequent arterial rupture and pseudoaneurysm formation. they had a one- to two-week history of chills and fever, and blood cultures were positive for salmonella. pulsatile, tender abdominal masses developed in two patients with aortic infection while they were hospitalized. the third patient's femoral artery infection presented as a painful swelling behind the knee. arteriography demonstrated large vessel rupture with pseudoaneurysm formati ...1978708237
[live vaccine against salmonellosis in swine].a live vaccine was produced against salmonellosis in pigs, using a mutant strain 3--297 of salmonella cholerae suis. the vaccine proved fully innocuous at the rate of 1 cm3 to guinea pigs and at 2-5 ml to pigs, and 15 days following injection the vaccinal strains was no longer isolated from the test animals. guinea pigs injected singly at the rate of 1cm3 of the vaccine and infected on the 12th day with a pathogenic salmonella cholerae suis strain died up to 12 per cent (the control ones up to 9 ...1978749326
[nitrosoguanidine induction of mutants in salmonella cholerae suis].on treatment two strains of salmonella cholerae suis with nitrosoguanidine and using ledeberg's contact technique, some 100 colonies of one of the strains were obtained, seven out of which were auxotrophic. these seven mutants were auxotropic, of which three -- to tyrosine, two -- to adenine, one -- to glutamic acid and one -- to histidine. the mutant of the second salmonella cholerae suis strain is auxotrophic to tyrosine, xanthine and adenine. the colonies of these mutants are smaller. their g ...1978751321
neurologic disease in naturally occurring salmonella choleraesuis infection in pigs.a granulomatous meningoencephalomyelitis was seen in 17 feeder pigs submitted for diagnostic evaluation from eight farms. vasculitis and destructive microgranulomas were widespread in both brain and spinal cord. occasionally there were microabscesses. salmonella choleraesuis var. kunzendorf was isolated from six of the eight groups, and there were lesions of acute salmonellosis in all animals examined. in three of the affected herds the principal disease manifestation was neurologic.1977855141
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