Publications

adherence, enterotoxigenicity, invasiveness and serogroups in campylobacter jejuni and campylobacter coli strains from adult humans with acute enterocolitis.	two hundred campylobacter jejuni and campylobacter coli strains from the same number of adult swedish patients with acute enterocolitis were tested regarding adherence to and invasiveness in hep-2 cells and for enterotoxigenicity by the cho-cell assay. the serogroup characteristics, heat-stable and heat-labile, for each strain were also investigated. eighty-four percent of the strains were classified as c. jejuni and 16 percent as c. coli. all of the strains were adherent to hep-2 cells, 39% wer ...	1990	2302354
horizontal transmission of campylobacter jejuni amongst broiler chicks: experimental studies.	horizontal transmission of campylobacter jejuni was investigated in campylobacter-free broiler chicks. one hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml c. jejuni. colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%) within 7 days], dependent on c. jejuni strain and inoculum size but independent of chick age. groups of 5-24 chicks in isolators were exposed to c. jejuni-contaminated water or co ...	1990	2307180
isolation and antimicrobial susceptibility of campylobacter coli and campylobacter jejuni from slaughter hogs.	cultural examination of cecal contents from 109 market weight hogs slaughtered in prince edward island during may-july 1988 yielded 62 isolates of campylobacter coli and seven campylobacter jejuni. a commercial latex agglutination test helped to confirm the identification of campylobacter. when tested against four drugs: erythromycin, tetracycline, kanamycin and ampicillin, 11 isolates showed multiple resistance. resistance to erythromycin was seen in 19% and 28.6% of campylobacter coli and camp ...	1990	2308529
cholera-like enterotoxin in certain campylobacter jejuni strains: some observations.	fourteen human isolates of campylobacter jejuni (13 pathogenic + 1 non-pathogenic) and two animal isolates were studied for the production of a toxin immunologically related to the heat-labile enterotoxin (lt) produced by a strain of escherichia coli of human (h) origin using an immuno-dot-blot assay with immunoaffinity purified antibodies against h-lt. polymyxin-b sulfate extracts of 72 hrs growth in eight of the 15 pathogenic strains were positive in immuno-dot-blot. six of these positive extr ...	1990	2308530
campylobacter jejuni: clinical and diagnostic value of serum antibody titres.	eighty patients with either bacteriologically confirmed campylobacter jejuni infection and/or an antibody titre value of at least 1:80, determined by elisa, were studied. a significant correlation was found between titre value and severity of symptoms (p = 0.015). although a correlation was noted between symptoms score and endoscopic abnormalities, this was not quite statistically significant (p = 0.053). comparison of patients with a titre of at least 1:1280 and those with lower titre values re ...	1990	2314520
prevalence and antibiogram of campylobacter jejuni in domestic animals in rural ghana.	a total of 134 samples of rectal and cloacal swabs taken from apparently healthy domestic animals that were in 43 of 76 homes located in rural ghana were examined for campylobacter jejuni. c. jejuni was isolated from 32.8% (44/134) of the animals. the highest isolation rate of 43.6% was from local domestic fowls, followed by goats (33.3%) and sheep (23%). this organism was not detected in the pigs, cats, and ducks. all 44 strains of this bacterium produced hydrogen sulphide and hydrolyzed hippur ...	1990	2317090
campylobacter jejuni infection occurring during pregnancy.	campylobacter infections occurring during pregnancy have been associated with spontaneous abortion, stillbirth, prematurity and neonatal sepsis, all ten campylobacter jejuni infections diagnosed in the approximately 24,000 pregnant women attending a 520-bed hospital between january 1984 and december 1988 were reviewed. nine women delivered healthy babies at term. in one case, campylobacter infection at 28 weeks of gestation was associated with premature labour and delivery with subsequent neonat ...	1990	2318219
enterotoxigenicity and frequency of campylobacter jejuni, c. coli and c. laridis in human and animal stool isolates from different countries.	campylobacter jejuni and c. coli strains were collected during three different years from adult patients with enterocolitis in sweden (n = 372) from 49 patients in kuwait, and campylobacter strains from hens from mexico, pakistan and sweden (n = 107) and swedish pigs (n = 47). c. jejuni was the predominant species in human and hen isolates, and c. coli in pigs c. coli was significantly more common in human isolates from sweden, and more common in hen isolates from pakistan, than in hens from swe ...	1990	2323537
production and characterisation of campylobacter jejuni enterotoxin in a synthetic medium and its assay in rat ileal loops.	a synthetic medium for production of campylobacter jejuni enterotoxin was developed for the purposes of its purification by modifying syncase medium, replacing sucrose with glucose, and supplementing with 0.025% sodium pyruvate, 0.25% sodium metabisulphite, 0.001% ferric chloride and 0.1% l-cysteine, adjusted to ph 6.7. culture filtrates of a human diarrhoeal and a chicken isolate, grown in this medium caused fluid accumulation ranging between 0.50-0.70 ml/cm of rat ileal loop. the kinetics of t ...	1990	2323551
efficacies of erythromycin and chloramphenicol in extinguishing fecal shedding of campylobacter jejuni in dogs.	oral treatment regimens of erythromycin stearate and chloramphenicol were evaluated in naturally infected laboratory colony dogs for their efficacies in extinguishing fecal shedding of campylobacter jejuni. of the 25 campylobacter-infected english foxhounds in the study, 9 were assigned to erythromycin treatment, 9 to chloramphenicol treatment, and 7 to no treatment. antimicrobials were administered for 12 days. all of the dogs that received erythromycin stearate ceased shedding c jejuni by the ...	1990	2329075
protection of breast-fed infants against campylobacter diarrhea by antibodies in human milk.	to investigate the role of breast-feeding in preventing diarrhea caused by campylobacter jejuni, we followed 98 mexican children prospectively for 2 years beginning at their birth. attack rates of diarrhea in children less than 6 months of age who were not fed human milk were 2.3 times greater than those in children of the same age who were fed human milk. breast-fed children remained free of diarrhea for a longer time than non-breast-fed children (p less than 0.0005). the diarrhea attack rate c ...	1990	2329419
culture confirmation of campylobacter spp. by latex agglutination.	a commercial latex agglutination test [meritec-campy (jcl), meridian diagnostics, cincinnati, ohio] was evaluated for identification of campylobacter jejuni, c. coli, c. laridis, and other campylobacter isolates. the test had 100% sensitivity in detecting c. jejuni and c. coli but low sensitivity with c. laridis isolates. c. upsaliensis strains reacted with the test. the test had 100% specificity for 101 non-campylobacter organisms.	1990	2332476
a domestic ferret model of immunity to campylobacter jejuni-induced enteric disease.	oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. orally infected animals developed agglutinin titers of 8 to 256 within 3 weeks, while those infected intravenously developed titers of 256 to 2,048. ferrets which had recovered from campylobacteriosis all developed high titers of agglutinating a ...	1990	2341180
a further study of the epidemiology of campylobacter jejuni infections.	epidemiological data on notifications of campylobacter jejuni infections is given for the christchurch health district area. the number of notifications rose steadily to a peak of 883 cases in 1987 (rate 261 per 100,000 population). christchurch shows consistently higher rates than the rest of new zealand. peak incidence is in the 0-4 age group; there is a high incidence amongst males and in rural areas; peak incidences in spring/summer; occupational risks have not been identified; chicken consu ...	1990	2342690
campylobacter jejuni in poultry giblets.	a total of 200 poultry giblets, 50 each of chickens, ducks, squab and turkeys, were examined for the presence of campylobacter jejuni. in chicken giblets, c. jejuni was isolated from gizzards, hearts, livers and spleens with incidences of 28%, 10%, 40% and 16% respectively while 24%, 6%, 36% and 10% of duck gizzards, hearts, livers and spleens were positive for the organism, respectively. c. jejuni was detected in 6% of squab gizzards, in 10% of squab livers but failed to be detected in squab he ...	1990	2346069
bismuth subsalicylate in the prevention of colonization of infant mice with campylobacter jejuni.	infant mice were used for the evaluation of the efficacy of bismuth subsalicylate (bss) in the prevention of the growth of campylobacter jejuni in the intestine. the mic90 of ten c. jejuni strains was 900 micrograms/ml. of three dosage regimens tested, continuous treatment before and after the bacterial challenge, mimicking the way bss is used in the prevention of traveller's diarrhoea, was the most effective. growth inhibition was dose dependent; the high dose of 2000 micrograms per day was mor ...	1990	2347383
[cryptosporidiosis in children].	during january through june 1988, oocysts of cryptosporidium were identified in fecal samples of 83 (7.7%) of 1073 children who presented with acute gastroenteritis. other intestinal pathogens were less common: salmonella 4.2%, e. coli 1.9%, shigella 2.6% and campylobacter jejuni 5.0%. cryptosporidiosis (c) was more common under the age of 5 years than over (8.4% vs 1.8%, respectively, p less than 0.01). in 6 (7.2%) children with c, another stool pathogen was found. frequent signs of c were diar ...	1990	2347532
[guillain-barré syndrome following campylobacter jejuni enteritis].	a 7-year-old boy nine days before onset of a guillain-barré-syndrome had had enteritis caused by campylobacter jejuni. the clinical signs were restricted to minor motor and sensory deficits in the limbs; 5 weeks after the onset of the syndrome, recovery was complete. so far, 16 cases of guillain-barré syndrome after a campylobacter enteritis have been reported in the literature.	1990	2348889
epidemiology of bacterial pathogens associated with infectious diarrhea in djibouti.	during a survey examining the causes of diarrhea in the east african country of djibouti, 140 bacterial pathogens were recovered from 209 diarrheal and 100 control stools. the following pathogens were isolated at comparable frequencies from both diarrheal and control stools: enteroadherent escherichia coli (eaec) (10.6 versus 13%), enterotoxigenic e. coli (etec) (11 versus 10%), enteropathogenic e. coli (epec) (7.7 versus 12%), salmonella spp. (2.9 versus 3%), and campylobacter jejuni-c. coli (3 ...	1990	2351738
in vitro susceptibility of campylobacter jejuni and campylobacter coli isolated in austria to erythromycin and ciprofloxacin.	more than 200 strains of campylobacter (c.) jejuni/coli isolated in 1985 and 1987/88 from human fecal specimens were tested for their susceptibility to erythromycin and ciprofloxacin. their mic90 as assessed by agar dilution tests was 2.0 and 0.5 mg/l, respectively. thus, all strains were regarded as susceptible to ciprofloxacin. with 2 out of 55 strains of c. coli the mic of erythromycin was 8.0 mg/l. therefore, only 3.6% of the c. coli strains were resistant to erythromycin. all 209 strains of ...	1990	2360966
bacterial aetiology of infantile diarrhoea in papua new guinea.	bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ...	1990	2362308
serogroups of campylobacter fetus and campylobacter jejuni isolated in cases of ovine abortion.	of 38 aborted ovine fetuses from 23 sheep flocks 29 c. fetus subsp. fetus and 22 c. jejuni were isolated and examined biochemically and serologically for heat-stable antigens. serologic examinations were carried out by passive haemagglutination test. in case of c. fetus subsp. fetus strains alkaline antigen extraction was used. antisera to two serogroups of c. fetus and to penner serotype reference strains 1 to 60 were produced in rabbits. abortion was caused in 18 (78.3%) flocks by c. fetus sub ...	1990	2363324
evidence of reinfection with multiple strains of campylobacter jejuni and campylobacter coli in macaca nemestrina housed under hyperendemic conditions.	a prospective bacteriologic study of 18 infant pig-tailed macaques (macaca nemestrina) housed in a nursery facility in which campylobacter spp. are endemic was undertaken to determine the epidemiology of infection and reinfection. the isolates of campylobacter jejuni and c. coli cultured from 8 of the 18 infants were characterized by serotyping, dna hybridization, and polyacrylamide gel electrophoresis protein profiles. the chronology of infection was indicative of multiple reinfections with dif ...	1990	2365455
infection of adult syrian hamsters with flagellar variants of campylobacter jejuni.	two variants of campylobacter jejuni in1 differing in the expression of flagella, in1 (fla+ mot+, wild type) and in1-nm (fla- mot-), were tested for their ability to establish infection in adult hamsters. animals were challenged intracecally with 2 x 10(9) to 5 x 10(10) cfu and monitored for evidence of infection. none of the challenged animals developed illness. there was a significant difference, however, in the ability of in1 to infect hamsters (35 of 43) compared with that of in1-nm (1 of 42 ...	1990	2365459
[erythema nodosum in campylobacter jejuni colitis].	we report the case of a 27-years-old woman with erythema nodosum associated with ulcerative colitis due to campylobacter jejuni as diagnosed by serology, endoscopy and histology. this case indicates a hitherto rarely described etiology of erythema nodosum.	1990	2367840
partial purification and characterization of the enterotoxin produced by campylobacter jejuni.	campylobacter jejuni enterotoxin was partially purified from culture supernatant. the purified fraction after gel filtration indicated three bands at 68, 54, and 43 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). this fraction enhanced the adenylate cyclase activity of hela cell membranes by 1.5-fold over that of the control. the study with anti-cholera toxin immunoglobulin g (igg) and ganglioside affinity column chromatographies revealed that the eluent from ...	1990	2370099
polynucleotide sequence relationships among flagellin genes of campylobacter jejuni and campylobacter coli.	dna probes that encode a complete flagellin gene and various internal regions of the campylobacter coli vc167 flagellin genes were hybridized to 30 strains of c. coli or c. jejuni from 20 different lior serogroups. the results indicated a high overall degree of homology among all of the strains examined. although the most variable regions occurred within the middle of the gene, significant dna homology was observed among many serogroups in this region of the molecule.	1990	2370114
very long term diarrhoea due to campylobacter jejuni.		1990	2371198
waterborne campylobacter jejuni epidemic in a finnish hospital for rheumatic diseases.	a waterborne campylobacter jejuni outbreak in the rheumatism foundation hospital in heinola, finland, in november-december 1986 is described. 32 patients and 62 members of the staff developed gastrointestinal symptoms. c. jejuni heat-stable serotype 45 was isolated from the faeces of 32 enteritis patients and from none of the controls. no other enteropathogens were found. positive serological responses to c. jejuni acid extract antigen were detected by enzyme immunoassay in 34% of the symptomati ...	1990	2371546
campylobacter jejuni versus campylobacter coli in developing countries: how accurate are prevalence estimates?		1990	2373882
[spontaneous bacteremia caused by campylobacter jejuni].		1990	2377784
urea protects helicobacter (campylobacter) pylori from the bactericidal effect of acid.	colonization of the stomach with helicobacter (campylobacter) pylori is common in patients with duodenal ulcer disease, which is known for its high acid secretion. although the bacterium is usually isolated by culture of a gastric biopsy specimen, viable organisms may sometimes be found in the acidic gastric juice. it was postulated that urease, by generating ammonia, protected h. pylori from acid. to test this hypothesis, the ph susceptibility of h. pylori, proteus mirabilis, and the urease-neg ...	1990	2379775
elisa for campylobacter jejuni antibodies in israeli children with diarrhea and in healthy soldiers.	an elisa test was employed to detect campylobacter jejuni antibodies, using as antigen the glycine-hcl buffer extract of a local isolate. the antibody response (igg, iga and igm) was examined in a group of 32 children (age 0-12 years) with c. jejuni enteritis diagnosed by the isolation of the agent. controls were represented by two groups of children matched according to their age and sex: a) children with enteritis of unknown etiology, and b) healthy children. in addition, 66 healthy soldiers ( ...	1990	2380033
dna probe culture confirmation assay for identification of thermophilic campylobacter species.	we studied the ability of a new dna probe-based assay system to correctly identify isolates of the thermophilic campylobacters campylobacter jejuni, c. coli, and c. laridis grown in vitro. we examined 424 organisms, including 214 campylobacter isolates and 210 other aerobic and anaerobic isolates. the probe assay, which uses a new homogeneous system in which all reactions take place within a single tube, demonstrated 100% accuracy, producing neither false-positive nor false-negative results. the ...	1990	2380357
purification, characterization, and localization of a protein antigen shared by thermophilic campylobacters.	a protein antigen with an apparent molecular weight (mr) of 31,000 was isolated from 0.2 m glycine hydrochloride (ph 2.2) extracts of a typical human fecal isolate, campylobacter jejuni vc74. the protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving with a superose 12 column. isoelectric focusing under nondenaturing conditions indicated a pi of 9.3, and amino acid composition analysis showed that the protein was unusually rich i ...	1990	2380360
use of an alkaline phosphatase-labeled synthetic oligonucleotide probe for detection of campylobacter jejuni and campylobacter coli.	a commercially available synthetic nucleic acid probe (snap) conjugated to alkaline phosphatase was compared with standard culture techniques for detecting campylobacter species. the snap was able to detect either 5 ng of c. jejuni dna or 10(5) cfu of bacteria. the snap could also detect dna extracted from 10(5) cfu in mock-infected stool samples. the snap detected c. jejuni and c. coli but showed no reactivity with c. laridis, c. fetus subsp. fetus, c. fetus subsp. venerealis, c. fennelliae, "c ...	1990	2380381
peritonitis caused by campylobacter jejuni and serologically confirmed in a patient being treated with continuous ambulatory peritoneal dialysis.	a case of campylobacter jejuni peritonitis in a patient being treated with continuous ambulatory peritoneal dialysis is described. the significance of the organism isolated from our patient was initially doubtful because of a rapid defervescence of symptoms with minimal specific antibiotic therapy, lack of a preceding diarrhoeal illness and the time taken to isolate and identify the organism. serial serum igm, iga and igg antibody estimations clearly showed an acute seroconversion confirming tha ...	1990	2384683
isolation of campylobacter jejuni from the eggs and organs in experimentally infected laying japanese quails (coturnix coturnix japonica).		1990	2385046
in vitro susceptibility of campylobacter jejuni to rokitamycin.	the susceptibilities of 100 clinical isolates of campylobacter jejuni to rokitamycin, a new macrolide, and eight other oral antibiotics were tested by using a broth microdilution method. rokitamycin demonstrated antibacterial activity that was higher than those of fosfomycin and the tetracyclines and comparable to those of erythromycin and clindamycin, whereas it was slightly less active than the quinolones.	1990	2386373
investigation of seemingly pathogen-negative diarrhoea in patients infected with hiv1.	thirty three consecutive patients infected by human immunodeficiency virus type 1 (hiv1) with persistent diarrhoea which remained undiagnosed after microbiological examination of six stool samples and rectal histology were investigated for malabsorption. all had xylose and schilling tests, distal duodenal biopsy, comprehensive barium studies, microbiological examination of six further stool samples, and repeat rectal histology. a microbiological or histological diagnosis of infection was made in ...	1990	2387512
surface array proteins of campylobacter fetus block lectin-mediated binding to type a lipopolysaccharide.	campylobacter fetus strains with type a lipopolysaccharide (lps) and a surface array protein layer (s+) have been found to be pathogenic in humans and animals. spontaneous laboratory mutants that lack surface array proteins (s-) are sensitive to the bactericidal activity of normal human serum. the ability of lectins to determine the presence of the s-layer and differentiate lps type was assessed. we screened 14 lectins and found 3 (wheat germ agglutinin, bandeiraea simplicifolia ii, and helix po ...	1990	2387622
[clinical evaluation of norfloxacin in children].	oral new quinolone, norfloxacin (nflx, am-715), was evaluated for its safety, efficacy and pharmacokinetics in children. 1. nflx was effective in 88.0% of 25 cases infected with haemophilus influenzae, pseudomonas aeruginosa, escherichia coli, campylobacter jejuni, staphylococcus aureus including methicillin-resistant strains, and other bacteria. 2. after single oral administration of 50 mg and 100 mg nflx tablet at fasting, mean peak values of serum concentration were 0.35, 0.48 microgram/ml an ...	1990	2391750
[laboratory and clinical studies on norfloxacin in the pediatric field].	in bacteriological, pharmacokinetic and clinical studies on norfloxacin (nflx, am-715), the following results were obtained: 1. antibacterial activity of nflx, nalidixic acid (na), amoxicillin (ampc), cefaclor (ccl), erythromycin (em) and fosfomycin (fom) against clinically isolated bacteria was examined, and it was found that mic80 of nflx against staphylococcus aureus was 3.13 micrograms/ml, thus nflx exhibited stronger antimicrobial activity than na, ampc, ccl, em and fom. nflx also showed go ...	1990	2391752
[laboratory and clinical studies on norfloxacin in pediatrics].	norfloxacin (nflx, am-715) is a new quinolone antibacterial agent. in the present study, we carried out laboratory and clinical investigations on nflx in the field of pediatrics. the obtained results are summarized as follows. 1. nflx was very active against escherichia coli, salmonella sp., klebsiella sp., enterobacter sp. and pseudomonas aeruginosa among gram-negative rods. antibacterial activities of nflx against gram-positive cocci were lower than those against gram-negative rods but superio ...	1990	2391753
[bacteriological and clinical studies on norfloxacin in the field of pediatrics].	bacteriological and clinical studies on norfloxacin (nflx) were carried out in the field of pediatrics, and the results obtained are summarized as follows: 1. the mics of nflx against clinically isolated organisms were determined to compare with mics of nalidixic acid, amoxicillin, cefaclor, erythromycin and fosfomycin. nflx had low mics against both gram-positive and gram-negative bacteria and, particularly, showed higher antimicrobial activity to escherichia coli, salmonella sp., klebsiella pn ...	1990	2391755
[clinical effect of norfloxacin in pediatric field].	we have investigated bioavailability, clinical efficacy, side effect and antimicrobial activity of norfloxacin (nflx) to evaluate the efficacy and safety in the pediatric field. results are summarized as follows 1. after oral administration of 100 mg, tmax was 2 hours and t 1/2 were 4.2 and 2.6 hours in 2 cases. 2. in 10 cases of urinary tract infection (uti) and 4 cases of intestinal tract infection (iti), clinical efficacy rate was 100% (14/14). 3. in uti, causative bacteria were all eradicate ...	1990	2391759
[clinical evaluation of norfloxacin in pediatrics].	we have evaluated the effectiveness and safety of norfloxacin (nflx) in 18 children with infectious diseases. doses ranging from 5.6 to 18.8 mg/kg/day for t.i.d. or q.i.d. were used. the causative bacteria were campylobacter jejuni in 3 cases, salmonella typhimurium in 1, staphylococcus aureus in 2, haemophilus influenzae in 1 and unknown in 11 cases. except 1 strain of s. aureus, all the bacteria mentioned above were eradicated. clinical effects were excellent in 8, good in 8, poor in 2, and th ...	1990	2391762
invasion-related antigens of campylobacter jejuni.	a hep-2 cell culture model was used to investigate the antigens required for epithelial cell penetration by campylobacter jejuni. penetration of hep-2 epithelial cells by c. jejuni was significantly inhibited (p less than .05) with c. jejuni lysate and a monoclonal antibody (mab 1b4) in competitive inhibition assays. immunogold electron microscopy revealed that mab 1b4 bound to the flagella and cell surface of low-passage (invasive) c. jejuni m 96, whereas only the flagella of high-passage (noni ...	1990	2401792
use of non-radioactive dna probes for detection of campylobacter jejuni and campylobacter coli in stool specimens.	dna probes specific for c. jejuni (pdt1720 containing a 1475 base pair fragment) and for c. jejuni and c. coli (pdt1719 containing a 1845 base pair fragment) were isolated from a bacteriophage lambda gt11 genomic library of c. jejuni, using antiserum prepared against a 46 kda major outer membrane protein of c. jejuni. the two probe-fragments had different restriction maps and were only moderately related by dna hybridization analysis. a non-radioactive labelling kit which consisted of alkaline p ...	1990	2402249
serogroups of campylobacter jejuni from man and animals.	a total of 186 campylobacter strains from aborted calf and sheep fetuses, from scouring dogs, rabbits and man, and from retailed poultry were isolated and examined biochemically and serologically for heat stable antigens. immune sera were produced in rabbits against penner reference strains from 1 to 60, and against two field isolates. out of 186 biochemically tested strains 179 (96.2%) proved c. jejuni and only 6 (3.2%) c. coli. one strain has been identified as c. laridis. in cattle and sheep ...	1990	2402970
microbiological aspects of helicobacter pylori (campylobacter pylori).	the human gastric pathogen campylobacter pylori has recently been reclassified as helicobacter pylori, and a related spiral bacterium found in the stomach of ferrets has been designated helicobacter mustelae. the general microbiological features of helicobacter pylori are delineated here, with details of phenotypic differences between helicobacter pylori and helicobacter mustelae; comparisons are made with wolinella succinogenes and campylobacter jejuni. the helicobacter organisms possess an ext ...	1990	2406141
diarrhea in developed and developing countries: magnitude, special settings, and etiologies.	diarrheal diseases are major causes of morbidity, with attack rates ranging from two to 12 or more illnesses per person per year in developed and developing countries. in addition, diarrheal illnesses account for an estimated 12,600 deaths each day in children in asia, africa, and latin america. the causes of diarrhea include a wide array of viruses, bacteria, and parasites, many of which have been recognized only in the last decade or two. while enterotoxigenic escherichia coli and rotaviruses ...	1990	2406855
epidemiology of travelers' diarrhea and relative importance of various pathogens.	each year 12 million persons travel from an industrialized country to a developing country in the tropics or subtropics. these travelers experience a high rate of diarrhea caused by a wide variety of enteric pathogens acquired by ingestion of contaminated food or water. one or more pathogens can be found in the stool of a majority of ill individuals. enterotoxigenic escherichia coli generally are the most frequently identified pathogens, having been found in a median of 42% of travelers' diarrhe ...	1990	2406860
dna restriction digest and ribosomal rna gene patterns of campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of united kingdom outbreak strains.	dna restriction endonuclease (hae iii and hind iii) total digest and 16s and 23s ribosomal (r)rna gene patterns (ribopatterns) were determined for 18 isolates of campylobacter jejuni from three separate outbreaks of diarrhoea in the north of england. strains were also characterized by biotyping, serotyping and phage typing. comparisons of the dna patterns by visual and numerical methods revealed five distinct strain groupings with clear differences between isolates from different outbreaks as we ...	1990	1698647
detection of antibodies to helicobacter pylori cell surface antigens.	serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ...	1990	1699265
[the specificity of campylobacter jejuni antigens in immunoenzyme analysis].	the surface antigen has been obtained from c. jejuni culture and its immunochemical analysis has been carried out by the methods of immunoelectrophoresis, double diffusion with homologous and heterologous rabbit sera. the protein profile of this antigen has been studied by means of vertical electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, and the molecular weight of the protein components of the antigen has been determined. the presence of anti-campylobacter antibodies has been ...	1990	1711263
[rotavirus diarrhea in children hospitalized in wrocław clinics].	studies have been performed in 250 children aged 0 to 3 years suffering from diarrhoea. rotaviruses have been founded in 25% of cases, most frequently in children aged 0 to 3 months (29.6%), and 6 to 12 months (21.8%). frequency of rotavirus infections was highest during february. in 26% of subjects studied the coexisting infection with enteropathogenic escherichia coli strains or salmonella and campylobacter jejuni has been observed. the rotazyme ii test is more sensitive than the rotalex and r ...	1990	1965233
guillain-barré syndrome and campylobacter jejuni infection.		1990	1971411
jackdaws as potential source of milk-borne campylobacter jejuni infection.		1990	1971891
bird attack on milk bottles: possible mode of transmission of campylobacter jejuni to man.	a case-control study was carried out to test the hypothesis that the rise in the rate of campylobacter jejuni infection in the brigend area of south wales during may was due to the consumption or handling of milk from bottles that had been attacked by birds. 32 of 36 cases meeting the case definition were interviewed, along with 2 controls per case, matched for age, sex, and area of residence. there were strong associations between campylobacter infection and doorstep delivery of milk bottles, a ...	1990	1978882
restriction fragment length polymorphism for the identification of campylobacter jejuni-isolates.	companion animals ("pets") are occasionally carriers of organisms pathogenic for man. in the present, study fecal samples of clinically inapparent animals with direct contact to 204 patients, suffering from campylobacter enteritis, were investigated for c. jejuni or c. coli (cjc). cjc positive animals were seen in the environment of only five patients (= 2.4%). by comparison of biotypes and serotypes of thermostable and thermolabile antigens from human and animal isolates no clear epidemiologica ...	1990	1982490
saskatchewan. prevalence of campylobacter jejuni in pronghorns and mule deer in southern saskatchewan.		1990	17423564
prevalence of campylobacter jejuni in ranch mink at pelting: cultural, serological, and histological evidence of infection.	this survey of 500 mink on three wisconsin ranches at pelting gives an estimate of the prevalence of campylobacter jejuni in the feces of clinically normal animals. on ranches 1 and 2, which used wet feed, c. jejuni was isolated by colon content culture from 7% and 32% of mink one year, and 43% and 13% the next year; the 200 bile samples tested were culture-negative. on ranch 3, which fed a pelleted ration, the organism was never isolated. among culture-positive mink tested, 22 of 55 had bacteri ...	1990	17423585
campylobacter jejuni abortions in two beef cattle herds in saskatchewan.	abortions, accompanied by placental retention and weight loss, occurred during february and march in 19% of 120 and 10% of 108 beef cows and heifers on two neighboring ranches in southern saskatchewan. a diagnosis of campylobacter jejuni abortion was made based on lesions of necrotizing and suppurative placentitis and fetal bronchopneumonia in association with the culture of large numbers of c. jejuni from placentas and fetal tissues.campylobacter jejuni was isolated with variable frequency from ...	1990	17423586
the isolation rate of escherichia coli 0157:h7 in toronto and surrounding communities.	verocytotoxin-producing strains of escherichia coli, most often serotype 0157:h7, have been associated with both sporadic and epidemic diarrheal disease in canada. in order to determine the isolation rate of e coli 0157:h7 in outpatients with diarrhea, all stool specimens submitted for culture to med-chem laboratories in metropolitan toronto between june 1988 and september 1989 were cultured on macconkey-sorbitol agar in addition to standard enteric media. a total of 46 (0.3%) of 16,125 stool sp ...	1990	22553432
human antibody response to campylobacter jejuni flagellin protein and a synthetic n-terminal flagellin peptide.	we measured isotype-specific human antibodies directed against campylobacter jejuni native flagellin and a synthetic peptide derived from the n-terminal amino acid sequence of the protein by using a microdilution enzyme-linked immunosorbent assay (elisa). serum samples from patients with gastrointestinal infection caused by c. jejuni (n = 20) and control samples (number from normal subjects = 20; number from patients with diarrhea other than campylobacter = 20) were tested in this assay. serum s ...	1989	2584372
application of different chromosomal dna restriction digest fingerprints to specific and subspecific identification of campylobacter isolates.	eleven strains comprising representatives of different subspecies, biotypes, and serotypes of campylobacter jejuni and reference strains of c. coli, c. fetus subsp. fetus, c. hyointestinalis, and c. sputorum subsp. sputorum were studied to assess the utility of different dna profiles for measuring fine differences between allied bacteria. strains were compared by analyses of haeiii and xhoi digest patterns of chromosomal dna and southern blot hybridization patterns of xhoi digests obtained with ...	1989	2584382
"campylobacter upsaliensis" isolated from cats as identified by dna relatedness and biochemical features.	campylobacter spp. were isolated from the feces of 20 (58%) of 53 asymptomatic cats during routine physical examination while the cats were maintained in an accredited quarantine facility. fifteen of these campylobacter spp. were identified phenotypically as campylobacter jejuni, and two were identified as c. coli. dna-dna hybridization (hydroxyapatite method) was used to confirm the identification of three thermotolerant catalase-negative isolates. they were 80 to 100% related to each other and ...	1989	2584385
[campylobacter infection and campylobacter excretion in turkeys].	five turkey poults at an age of 27 days were infected with campylobacter jejuni strain. additional five poults were infected by contact. turkeys infected by contact and artificially were necropsied 1 and 3 months later, respectively. the histological examination of the liver revealed degenerative changes in contact birds and a proliferation of the connective tissue and bile-ducts in two of the oral infected poults. campylobacter spp. were excreted daily during the first two weeks and afterwards ...	1989	2590155
heat resistance of campylobacter and yersinia strains by three methods.	two methods of determining the heat resistance of bacteria, a glass cup and a test tube method, were compared with a method using capillary tubes. three strains of yersinia enterocolitica, one of campylobacter jejuni and two of c. coli were tested in physiological saline. the differences between the results obtained by the glass cup method and the reference method were not statistically significant for five strains and were small also for the other, a yersinia strain. d values obtained by the gl ...	1989	2592291
[campylobacter jejuni].		1989	2595720
interaction of campylobacter jejuni with extracellular matrix components.	the adhesion of three strains of campylobacter jejuni to coverslips and microwells coated with isolated extracellular matrix components, fibronectin, laminin and types i, iii, iv and v collagens was studied. fibronectin mediated the adherence of c. jejuni, but there were differences in the binding capacities of the strains. type i, iii and v collagens mediated very strongly the attachment of two strains of c. jejuni. all three strains attached weakly to basement membrane-specific type iv collage ...	1989	2597700
[infectious diarrhea in the adult].	infectious diarrhoeas are usually divided into two types; toxinogenic and invasive. invasive diarrhoeas are copious and responsible for dehydration which is the principal clinical sign; mucosal lesions and bacteraemia are absent. the most typical of toxinogenic diarrhoeas is cholera, but enterotoxicogenic e. coli and aeromonas infections have similar clinical features. in invasive diarrhoeas the responsible microorganisms penetrate into the epithelial cells of the intestine, producing fever and ...	1989	2602888
response of the chick embryo to live and heat-killed campylobacter jejuni injected into the yolk sac.	graded doses of live and heat-killed cells of campylobacter jejuni were injected into the yolk-sac of 5-day-old chick embryos, and the 50% lethal dose (ld50) was determined 7 days later. a strain dependent virulence was seen. in the diluted series of cultures the ld50 values for live campylobacter ranged from 10(6) c.f.u. beyond the last dilution showing growth, that is to less than one organism per embryo. when the 22 strains were tested as heat-killed cells, the chick embryo ld50 values retain ...	1989	2606163
plasmids and serogroups in campylobacter jejuni.	for epidemiological purposes identification of campylobacter strains is usually based on surface antigen characteristics. two different systems, one for heat-stable (hs) and one for heat-labile (hl) antigen have dominated. in earlier studies we found a great variability in the two antigen systems. the aim of the present investigation was to analyse the frequency of plasmids in campylobacter strains in the light of their possible use as an epidemiological tool as well as the relation between the ...	1989	2611024
in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea.	the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ...	1989	2613604
adhesion to hela cells of campylobacter jejuni and c. coli outer membrane components.	adhesion of campylobacter jejuni and c. coli to epithelial cells is thought to be a decisive step in enteritis. in this work, we tried to determine which bacterial components are responsible for this phenomenon. outer membrane (om) extracts were prepared from strains of c. jejuni (3 strains) and c. coli (2 strains). these strains had been isolated from stools of febrile patients with diarrhoea and were able to adhere to hela cells in culture. after incubation of bacterial om extracts with hela c ...	1989	2616891
prevalence of campylobacter jejuni in selected domestic and wild birds in louisiana.	prevalence of campylobacter jejuni was determined in a selected population of domestic and free-living birds submitted for necropsy to the louisiana state veterinary medical diagnostic laboratory. the 445 cases examined included 13 orders of birds and yielded c. jejuni in 45 cases, representing an isolation rate of 10.1%. prevalence was highest in galliformes (25.2%), followed by anseriformes (12.9%) and columbiformes (8.3%). only one isolation was made out of 179 psittaciformes examined. penner ...	1989	2619661
[cytotoxin adhesion and invasiveness of campylobacter jejuni from children with diarrhea: assay and correlation with clinical manifestations].	some potentially pathogenic properties have been identified for campylobacter jejuni in vitro. the cell-free filtrates of 42 strains, (28 isolated from clinical cases of diarrhea, 12 from domestic animals and 2 from healthy children) were investigated. 30 of the 42 were cytotoxin producers, and the strains from clinical cases were more frequent than those from other sources (p less than 0.05). meanwhile, 25 of the 42 strains exhibited adhesion and invasiveness with hela cell line in vitro. it is ...	1989	2630022
glomerulonephritis, pulmonary hemorrhage and anemia associated with campylobacter jejuni infection.	a 5-year-old girl suffered campylobacter jejuni enteritis. over the ensuing weeks she developed glomerulonephritis, pulmonary hemorrhage and anemia. renal biopsy revealed immune-complex mediated, crescentic glomerulonephritis. campylobacter jejuni antigen was identified in the glomeruli suggesting a causal role of campylobacter jejuni in the disease process.	1989	2631667
systemic spread of campylobacter jejuni after intravenous infections.	mice were infected intravenously with campylobacter jejuni in order to study systemic translocation of this vibrio, as well as the interactions between bacteria and the host's defense mechanisms. it was found that granulocytes phagocyte c. jejuni in the bloodstream and that phagocytosis could be stimulated with lps-pretreatment or, less effectively, opsonizing antibodies. it could also be demonstrated that these circulating 'infected' granulocytes are eliminated from the bloodstream mostly by th ...	1989	2631866
endotoxic activity and enterotoxigenicity of human strains of campylobacter jejuni isolated from patients in a nigerian hospital.	limulus gelation assay and dermal schwartzman reaction provided a sensitive and reproducible means of testing the endotoxic activity of hospital strains of campylobacter jejuni in lagos, nigeria. all the 22 isolates of campylobacter jejuni tested for the limulus gelation assay were positive for the production of endotoxin. furthermore, the campylobacter suspensions caused a positive dermal schwartzman reaction in rabbits. the area of skin reaction was less extensive than that produced by escheri ...	1989	2632005
bacterial and viral causes of acute diarrhoea in children in kuwait.	bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ...	1989	2632640
[frequency and characteristics of campylobacter jejuni-coli diarrhea in tunisia].	because importance of campylobacter jejuni/coli in human disease, we studied frequency, clinical and biological symptoms of campylobacter enteritis. investigation has been realised on 216 cases of diarrhoea seen outside the hospital and aged from 4 days to 60 years, and 512 cases of hospitalised diarrhoea. outside the hospital, this bacteria has the same frequency as shigella and salmonella (3-5%). inside the hospital, it is rare. outside the hospital, this bacteria is isolated in babies and chi ...	1989	2633873
[serological diagnosis of campylobacter jejuni infections].	antibody level to campylobacter in 28 sera of patients of whom campylobacter infection was confirmed by germ isolation from feces was tested. the investigation was performed using passive haemagglutination technique and as antigens heated and acid glycine extraction prepared from homologous and reference strains. for the method used the heated antigen proved to be superior. out of 28 tested patients of whom 92.8% were children, 8 sera were positive, 9 doubtful and 9, derived mainly from neonates ...	1989	2640683
better growth of campylobacter jejuni using simple fortner's principle & candle extinction jar.	c. jejuni isolated from patients of childhood diarrhoea and chicken intestines were examined for better growth in both liquid and solid media under three different environments of candle extinction jar with or without an escherichia coli inoculated plate and three such plates in a sealed jar. better growth by one log in liquid media was obtained at 24 and 48 h with bacterial counts of about ca. 10(7) and ca. 10(9) respectively, and larger colony size at 24 h on solid medium by inclusion of a sin ...	1989	2644169
emerging role of quinolones in the prevention of gram-negative bacteremia in neutropenic cancer patients and in the treatment of enteric infections.	the pharmacokinetic and antibacterial characteristics of the fluorinated carboxyquinolones make them attractive candidates for the treatment and prevention of infections arising from the alimentary canal. norfloxacin and ciprofloxacin have been shown to suppress and eliminate the pool of potentially pathogenic aerobic gram-negative rods colonizing the alimentary canal of neutropenic patients with acute leukemia, thereby reducing infection-related morbidity and mortality due to gram-negative seps ...	1989	2646056
incidence and etiology of infantile diarrhea and major routes of transmission in huascar, peru.	community-based studies of diarrhea etiology and epidemiology were carried out from july 1982-june 1984 in 153 infants residing in a poor peri-urban community near lima, peru. study infants had nearly 10 episodes of diarrhea in their first year of life. diarrhea episodes were associated with organisms such as campylobacter jejuni, enterotoxigenic and enteropathogenic escherichia coli, shigella, rotavirus, and cryptosporidium. these organisms appeared to be transmitted to infants in the home thro ...	1989	2646919
characterization and antibiotic sensitivity of campylobacter jejuni and campylobacter coli isolated from children at the lagos university teaching hospital (luth), lagos, nigeria.	one hundred and thirteen rectal swabs and 87 faecal samples obtained from the paediatric casualty of the lagos university teaching hospital, lagos, nigeria were processed for campylobacter jejuni/coli. twenty-two (11%) of the specimens were positive for c. jejuni. biotyping results showed that nine (41%) and eight (36%) belonged to c. jejuni biotypes i and ii respectively, while three (14%) and two (9%) belonged to c. coli biotypes i and ii. serotyping results revealed that c. jejuni biotypes i ...	1989	2651685
[isolation of campylobacter jejuni and campylobacter coli from children with acute intestinal diseases].	in this work the data obtained in the examination of 338 children with diarrhea, aged 5 days to 14 years, are presented. the methods used for the collection of samples and their storage till the moment of inoculation are described. the possibility of using the microscopic examination of campylobacter-containing native feces is shown. the work resulted in the isolation of 85 c. jejuni and c. coli strains. as shown in this work, the isolation of campylobacter depended on the age of children and th ...	1989	2652954
campylobacter jejuni chromosomal sequences that hybridize to vibrio cholerae and escherichia coli lt enterotoxin genes.	campylobacter jejuni is one of the main etiologic agents of gastrointestinal illness in developing and developed areas throughout the world. isolation of enterotoxin-producing c. jejuni has been associated with clinical symptoms of a watery-secretory type of diarrhea. although physiological and immunological relatedness has been demonstrated between the c. jejuni enterotoxin (cjt), the vibrio cholerae enterotoxin (ct), and the heat-labile cholera-like escherichia coli enterotoxin (lt), nucleotid ...	1989	2653963
enteroaggregative escherichia coli associated with persistent diarrhea in a cohort of rural children in india.	a cohort of 452 rural children was followed longitudinally for 13 mo to ascertain the role of hep-2 cell adherent escherichia coli and other pathogens in causing acute (less than or equal to 14 d) and persistent (greater than 14 d) diarrhea. aeromonas, campylobacter jejuni, e. coli manifesting localized adherence to hep-2 cells and enterotoxigenic e. coli were significantly associated with acute diarrhea. e. coli strains that exhibit aggregative adherence, so-called enteroaggregative e. coli, a ...	1989	2656875
microbiologic and clinical study of acute diarrhea in children in aswan, egypt.	children with diarrhea presenting to a government rehydration center in aswan, egypt, were investigated to determine the etiology and clinical presentation of acute childhood diarrhea in southern egypt. among 126 outpatients and 25 inpatients with diarrhea (mean age 18 months), enterotoxigenic escherichia coli (etec) (17% of cases), cryptosporidium (9%), salmonella spp. (7%), campylobacter jejuni/coli (7%), and shigella spp. (5%) were the most common enteropathogens identified during the high in ...	1989	2658018
[susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates].	susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ...	1989	2664255
[neurologic involvement in campylobacter infections. 5 cases].	campylobacter are a newly recognized class of human infectious agents. campylobacter fetus subspecies fetus is a cause of sepsis in immunocompromized hosts. secondary neurological determinations, meningitis and meningoencephalitis appear to be rare. we report 2 cases, and 8 previously reported cases are reviewed. campylobacter jejuni appears to be a common bacterial pathogen causing a syndrome of enteritis. since 1982 it has been associated with guillain-barré syndrome (gbs). in one serological ...	1989	2664975
etiology of childhood diarrhea in korea.	to assess the role of recently recognized enteropathogens in childhood diarrhea in korea, 231 children with diarrhea admitted to and 104 children without diarrhea seen at the well-baby clinic or the outpatient department of hanyang university hospital in seoul, korea, were evaluated during a 14-month period. stools were cultured for bacterial pathogens, including enterotoxigenic (heat-labile and heat-stable enterotoxin-producing) and enteroadherent organisms. only those stools obtained from pati ...	1989	2666437
a one-year study on thermophilic campylobacters isolated from fecal specimens.	during a 12-month period, a systematic screening for thermophilic campylobacters was done on 5723 stool samples of hospitalized patients and out-patients who required stool culture tests for gastrointestinal disturbances. campylobacter strains were isolated in 130 samples (2.27%) and campylobacter jejuni (mainly biotype ii) was the species most frequently isolated. no pronounced seasonal pattern was evident and the age at which infection was more often observed was that from 6 to 24 months. eryt ...	1989	2674635
[antibacterial and bactericidal activities of japanese green tea].	we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ...	1989	2677434
serotypes & biotypes of campylobacter jejuni & c. coli from diverse sources in calcutta.	c. jejuni and c. coli isolates from diverse sources in calcutta were serotyped and biotyped according to the lior scheme. of the 55 strains examined, 85.5 per cent reacted with one or another of the 73 antisera available. this included the formation of two new serogroups, lio 67 and lio 76. c. coli serogroup lio 46 biotype ii was the most frequently encountered strains (14.5%), followed by c. coli serogroup lio 29, 55 biotype ii (10.5%) and c. jejuni serogroup lio 54, biotype i (5.5%). serogroup ...	1989	2680933
a study of campylobacter jejuni and campylobacter coli in a river system.	campylobacter isolates were detected in 82.1% of river water samples moderately polluted with organic wastes. in the majority of the samples less than 10 campylobacter cfu/100 ml were detectable. the presence of waterfowl and the faecal contamination from a poultry-farm resulted in considerable higher campylobacter contents (greater than 240/100 ml). correlation studies showed a high correlation coefficient (p less than 0.05) between the number of campylobacters and that of total coliforms. camp ...	1989	2684204