Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| respiratory syncytial virus. brief review. | 1985 | 3885919 | |
| practical recommendations for the detection of pediatric respiratory syncytial virus infections. | in our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ... | 1985 | 3897268 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| properties of a respiratory syncytial virus isolated from a sheep with rhinitis. | a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ... | 1985 | 3893243 |
| quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ... | 1985 | 3894575 |
| correlation of a disease scoring system with arterial po2 values in respiratory syncytial virus infection in calves. | the mean arterial po2 value measured in blood obtained by puncture of the brachial artery of 20 calves with acute clinical signs of a bovine respiratory syncytial virus (brsv) infection was 8.4 +/- 1.9 kpa. the values differed significantly from arterial po2 values of eleven healthy calves (mean 14.2 +/- 1.5 kpa). a disease scoring system is presented based on the type of respiration and the findings on auscultation. a high correlation (r = -0.87) was found between disease scores and arterial po ... | 1985 | 3925615 |
| respiratory syncytial virus outbreak at an institution for the mentally retarded--washington. | 1985 | 3932832 | |
| an outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward. | a small outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward during the period december 1982 to march 1983 is reported. it demonstrates that respiratory syncytial virus infection can cause serious lower respiratory tract infection even in patients without any previous history of chest disease. | 1985 | 4013906 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. | a new enzyme-linked immunosorbent assay (elisa) respiratory syncytial virus antigen detection kit (ortho diagnostics, inc., raritan, n.j.) was compared with virus culture and with the indirect fluorescent antibody test (fat) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. both uncentrifuged nasal washings and pellets from centrifuged split specimens were examined by elisa. the elisa was considered positive when the optical density was greater tha ... | 1985 | 3902879 |
| ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease. | aerosolized ribavirin was evaluated in the treatment of respiratory syncytial virus lower respiratory tract disease in 53 infants, 36 of whom had underlying diseases. of the total infants, 26 were studied in a double-blind, placebo-controlled manner; 14 received ribavirin and 12 received placebo, a water aerosol, for an average of five days. when the infants with bronchopulmonary dysplasia and congenital heart disease treated with ribavirin were compared with those receiving placebo, the treated ... | 1985 | 3903239 |
| etiology of community-acquired pneumonia in patients requiring hospitalization. | the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ... | 1985 | 4018066 |
| quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats. | the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ... | 1985 | 4020957 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| the childhood health effects of an improved water supply system on a remote panamanian island. | the incidence of diarrhea, respiratory disease, and skin infections was prospectively determined after the introduction of a system which distributed unlimited quantities of high quality fresh water to each of the 150 housing units on tupile, an island devoid of fresh water located off panama's caribbean coast and inhabited by 1,500 cuna indians. tupile residents used 7.1 liters of water/person/day compared to the 2.3 usage rate of inhabitants on achutupo, the control island. despite ready avail ... | 1985 | 4037183 |
| outbreak of respiratory syncytial virus in france. | a report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in france. twenty-three of 32 infants were infected (72%) despite infection control procedures. prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). the administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05). | 1985 | 4043060 |
| studies of passive immunotherapy for infections of respiratory syncytial virus in the respiratory tract of a primate model. | 1985 | 4045247 | |
| bovine respiratory syncytial virus. | the current knowledge is reviewed in regards to the importance of bovine respiratory syncytial virus in the bovine respiratory disease complex. the epidemiology, clinical disease, pathologic findings, pathogenesis, diagnosis, treatment, and prevention of this viral disease are discussed. | 1985 | 3907775 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| respiratory syncytial virus polypeptides. v. the kinetics of glycoprotein synthesis. | the cell-associated glycoproteins of respiratory syncytial virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). the time course of virus protein synthesis in hela cells revealed that the nucleocapsid protein (vpn41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. pulse-chase experiments with [3h]leucine or [35s]methionine demonstrated that the precursor for vgp48 and gp26 was an uncleaved protein of 70k (v ... | 1985 | 4009147 |
| serological and virological investigations of young children with acute respiratory syndrome associated with respiratory syncytial virus. | from january 1979 to march 1979, 341 young children from the metropolitan area of naples, italy, were hospitalized with respiratory virus disease. diagnosis of patients made from virus isolation and seroconversion indicate that the respiratory syncytial virus was a primary cause of this acute respiratory syndrome. | 1985 | 3967475 |
| experimental bovine respiratory syncytial virus infection in conventional calves: ultrastructural respiratory lesions. | the morphogenesis and repair of airway and alveolar injury induced by bovine respiratory syncytial virus (brsv) was studied ultrastructurally in conventional calves to characterize pulmonary cell types susceptible to viral infection and cytopathologic changes associated with infection. viral nucleocapsids and budding virions were present in tracheal and bronchial ciliated and nonciliated epithelial cells and mucous cells 3, 5, and 7 days after inoculation and in bronchiolar ciliated and noncilia ... | 1985 | 3994122 |
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural protein. | an mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ... | 1985 | 4009789 |
| comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus infections. | an indirect double antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and titration of serum antibodies to bovine respiratory syncytial virus (brsv). the elisa was compared with a complement fixation (cf) test and a test for virus neutralising antibody in serum (virus neutralisation [vn] test). testing sera collected in dairy herds revealed the closest correlation between the results of the elisa and the cf test with respect to brsv antibody titres. the v ... | 1985 | 4012035 |
| respiratory tract infections. goals for 1995. | goals to be identified for 1995, a decade hence, in the prevention, diagnosis, and management of respiratory tract infections may conveniently be divided into diagnostic goals and goals in therapy and prophylaxis. major diagnostic goals for bacterial, viral, and mycoplasmal infections of the respiratory tract focus on the development of systems to identify microbial components, such as specific antigens or segments of dna, using monoclonal antibody techniques or dna probes for hybridization. spu ... | 1985 | 4014289 |
| serologic studies of bovine respiratory syncytial virus in minnesota cattle. | serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. serum samples were obtained through the minnesota state-federal brucellosis laboratory and were collected over a 1-year period. results of this study revealed an antibody prevalence of 65.5% to bovine respiratory syncytial virus. the distribution of antibody titers is presented, as well as analysis of titers based on breed, sex, and age of the cattle. | 1985 | 4014838 |
| [incidence of respiratory syncytial virus infection in case records of a pediatric general medicine department]. | 1985 | 4021934 | |
| kinetics of synthesis of respiratory syncytial virus glycoproteins. | the synthesis of the two respiratory syncytial (rs) virus glycoproteins (vp66 and vp84) was examined under standard conditions and after treatment with tunicamycin and monensin. the protein backbone for vp66, the fusion protein (f1,2) is cotranslationally glycosylated to form f0, which is cleaved to form f1,2 by 20 min of chase. monensin treatment inhibited the cleavage of f0 over an 80 min chase period, indicating that this occurred late in the transit of f0 through the golgi apparatus or after ... | 1985 | 4031826 |
| cellular reactivity to respiratory syncytial virus in human colostrum and breast milk. | colostrum and breast-milk samples were taken from 23 mothers between 2 days and 7 weeks postpartum and were examined for the presence of cellular reactivity to respiratory syncytial (rs) virus using a lymphocyte transformation assay. positive responses were detected in nine of the 23 (39%) samples taken at 2-5 days postpartum, but this reactivity was undetectable at 3 weeks. positive responses developed in a further three mothers during the 3-7-week period of lactation, suggesting a response to ... | 1985 | 4045438 |
| severe respiratory disease in dairy cattle in new york state associated with bovine respiratory syncytial virus infection. | severe respiratory disease associated with bovine respiratory syncytial virus (brsv) infection has been identified in dairy cattle in new york state. the cases identified occurred in dairy calves and heifers. the disease was characterized in 4 animals by pathologic changes including interstitial pneumonia, necrotizing bronchiolitis with multinucleated syncytial epithelial cells and interstitial emphysema. brsv antigen was demonstrated in lung samples or was isolated in tissue culture in all 4 ca ... | 1985 | 4053607 |
| an epizootic of respiratory syncytial virus infection in a dairy herd. | respiratory syncytial virus was the cause of a severe epizootic of bovine respiratory tract disease. the virus, isolated from a sick cow during the epizootic, produced cytopathic effect in a bovine turbinate cell line 14 days after it was inoculated. additional support for the diagnosis came from the results of pathologic and serologic examinations. lesions consistently present were severe necrotizing bronchiolitis and epithelial syncytia projecting from bronchiolar and alveolar walls. also, in ... | 1985 | 4055489 |
| severe rsv bronchiolitis in an immunocompromised child. | a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child. | 1985 | 4064461 |
| bronchodilator response during acute viral bronchiolitis in infancy. | bronchodilator responsiveness was assessed by measuring specific respiratory conductance before and after inhalation of aerosolized bronchodilator in 50 infants who had acute bronchiolitis due to respiratory syncytial virus infection. thirty per cent of the infants showed an improvement in specific conductance. responders could not be differentiated from nonresponders by family histories of atopy, eosinophil counts, or immunoglobulin levels in blood and nasal secretions. eighty-three per cent of ... | 1985 | 4069797 |
| [infections with respiratory syncytial virus in infants and young children]. | 1985 | 4080005 | |
| [respiratory syncytial virus: rapid detection of antigen using monoclonal antibodies]. | 1985 | 4080006 | |
| spontaneous bovine respiratory syncytial virus infections in calves: arterial blood gas, ph and bicarbonate values. | arterial blood gas values of 12 calves in the acute stage of a bovine respiratory syncytial virus infection were compared with corresponding values of 11 healthy calves. the patients appeared to be severely hypoxic. mean (+/- sd) arterial oxygen tension (pao2) values of patients and healthy calves were 7.7 +/- 1.9 and 14.2 +/- 1.5 kpa, respectively. hypercapnia was present in only three of the diseased calves. these findings are in close agreement with findings in young children with respiratory ... | 1985 | 4090240 |
| respiratory syncytial virus infection in transported calves. | nasal swab samples were collected from calves on individual farms in tennessee and sequentially at an auction barn and at a feedlot to detect respiratory syncytial virus (rsv). in 1976, rsv was isolated from 5 of 225 calves at the auction barn and from 13 of 92 calves examined at the feedlot. of the 13 isolations, 11 were from calves with acute respiratory tract disease. most (14/18) calves infected with rsv were also shedding parainfluenza-3 virus in their nasal secretions. attempts to isolate ... | 1985 | 2417510 |
| cytotoxic t-cell response to respiratory syncytial virus in mice. | the role of the humoral and cellular arms of the immune response in protection against respiratory syncytial virus (rsv) infection and in the pathogenesis of the severe forms of this disease is poorly understood. the recent demonstration that some inbred mouse strains can be infected with rsv has opened the way to a detailed investigation of rsv immunity. we report here the finding of major histocompatibility complex-restricted, rsv-specific memory cytotoxic t cells in the spleens of balb/c and ... | 1985 | 2411951 |
| [natural killers and interferon in complicated influenza and other acute respiratory viral diseases in children]. | the results of the study on the functional activity of natural killers (nk) and serum interferon in 118 children ranging in age from 3 months to 5 years suffering from influenza and other acute respiratory viral diseases complicated with croup, pneumonia, and obstructive syndrome (66 children with influenza, 21 with parainfluenza, 16 with adenovirus infection, and 15 with respiratory syncytial virus diseases) are presented. the control group consisted of 22 normal children. decreased nk activity ... | 1985 | 2420069 |
| antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. | to study the antigenic characteristics of respiratory syncytial virus (rsv), we developed and evaluated monoclonal antibodies (mabs) to three strains of rsv: 11 to long, 4 to 18537, and 9 to a2. six of these mabs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. by the pattern of the reactions of these mabs to 16 strains of rsv in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. with ... | 1985 | 2579169 |
| cytotoxic lymphocytes in the lungs of mice infected with respiratory syncytial virus. | mice infected with respiratory syncytial virus (rsv) developed cytotoxic lymphocytes in the lungs, which lysed rsv-infected, but not uninfected cells. cytotoxic activity was greatest 7 to 9 days after infection, was virus-specific, mhc-restricted and abolished by treatment of lymphocytes with anti-thy 1.2 or with anti-lyt 2.2 sera and complement. there was a close temporal relationship between the appearance of these cytotoxic lymphocytes in the lung and clearance of virus. in contrast, rsv pers ... | 1985 | 2866227 |
| antigenic analysis of a putative new strain of respiratory syncytial virus. | a recent isolate (9320) of respiratory syncytial virus (rsv) that had been reported to differ from all previous strains was compared with rsv strains isolated over a period of 23 years by using the techniques of in vitro neutralization by specific antiserum and in vivo resistance to infection in cotton rats. the 9320 strain did not appear to be a novel antigenic variant and exhibited greater homology to several prototype strains than did an earlier isolate. the antigenic differences detected by ... | 1985 | 2579170 |
| a simple method for increased recovery of purified paramyxovirus virions. | a simple method involving vigorous agitation of infected cell monolayers prior to collection of culture medium is described to greatly increase the recovery of purified virions of measles virus, respiratory syncytial virus and human parainfluenza virus. vigorous agitation of the flasks containing monolayers of infected cells increased the recovery of purified virions by at least 3- to 10-fold as judged by the intensity of [35s]methionine labeled viral proteins on sodium dodecyl sulphate-polyacry ... | 1985 | 3001119 |
| nucleotide sequences of the 1b and 1c nonstructural protein mrnas of human respiratory syncytial virus. | the genes encoding the 1c and 1b mrnas of human respiratory syncytial (rs) virus are first in the order of viral transcription and encode nonstructural (ns) proteins of approximate molecular weights 14,000 and 11,000, respectively, estimated by gel electrophoresis. the complete nucleotide sequences of the 1c and 1b mrnas determined from several full-length cdna clones are described. the 1c and 1b mrnas contain 528 and 499 nucleotides, respectively, exclusive of poly(a), and encode proteins of 13 ... | 1985 | 2998021 |
| the disinfection of respiratory syncytial virus by isopropanol and a chlorhexidine-detergent handwash. | two of the products commonly used to disinfect the hands of nursing staff are 70% isopropanol and aqueous chlorhexidine-detergent solutions. these have been shown to rapidly destroy respiratory syncytial virus, and are therefore suitable for use where contagious spread of this virus is suspected. the virucidal component of the chlorhexidine-detergent preparation has been shown to be primarily the detergent base and not the chlorhexidine. | 1985 | 2859328 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| monoclonal antibodies for the rapid diagnosis of influenza a and b virus infections by immunofluorescence. | mouse monoclonal antibodies, directed against antigenic sites on influenza a and b viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. the influenza a antibodies were directed against nucleoprotein or matrix protein antigens and the influenza b antibodies against nucleoprotein and haemagglutinin antigens. the influenza a anti-matrix monoclonal antibody was found to give a stron ... | 1985 | 2865418 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| acute respiratory tract infections of children in hospital: a viral and mycoplasma pneumoniae profile. | respiratory specimens and blood were collected from all infants and children admitted with acute respiratory illness to a paediatric unit in christchurch from may to november (late autumn, winter and spring) 1983, to define the viral aetiological agents involved. a virus or mycoplasma pneumoniae was identified in 160 (50%) of 317 children studied by the rapid indirect immunofluorescence, virus culture and/or serological techniques. aetiological agents were detected in 71% of children with bronch ... | 1985 | 2991830 |
| antiviral effect of flavonoids on human viruses. | the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ... | 1985 | 2981979 |
| identification of respiratory virus antigens in middle ear fluids of children with acute otitis media. | antigens of respiratory viruses were tested by immunoassay of the middle ear fluids and nasopharyngeal secretions of 137 children with acute otitis media. the following were found: (1) an epidemic of infection with respiratory syncytial virus (rsv) caused a significant increase in the occurrence of acute otitis media. (2) fifteen percent of the children had rsv antigens in middle ear fluid, and in 7% rsv was the sole pathogen found. adenovirus antigens were found in the middle ear fluid of 3% of ... | 1985 | 2982961 |
| antiviral chemotherapy and chemoprophylaxis. | antiviral compounds have been developed for use in chemoprophylaxis and chemotherapy of a variety of infections in humans, including those caused by influenza viruses, respiratory syncytial virus, and herpesviruses. the efficacy of several of these compounds has been demonstrated in rigorously controlled trials. advances in molecular virology have led to the identification of biochemically defined, virus-specific functions that serve as appropriate targets for the future development of antiviral ... | 1985 | 2983421 |
| respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo. | recurrent infections with respiratory syncytial virus (rsv) have been well documented despite serological evidence of prior exposure of the host and the absence of clear evidence of antigenic variation of the virus. therefore, human mononuclear leukocytes, as well as purified lymphocytes and monocytes-macrophages, were exposed to rsv in vitro and examined for expression of viral antigens by using indirect immunofluorescence with monoclonal antibodies to rsv. rsv infected both human monocytes-mac ... | 1985 | 2931491 |
| detection of viral and chlamydial antigens in open-lung biopsy specimens. | the recovery of viruses and chlamydia trachomatis from cell cultures and the detection of their antigens in impression smears prepared from open-lung biopsy (olb) specimens from immunocompromised adults were compared. touch impression smears were prepared on three slides, each containing eight wells. olb tissue was homogenized (stomacher) and inoculated into mrc-5, primary monkey kidney, and mccoy cell cultures. the direct and indirect immunofluorescence (if) tests were used to detect antigens t ... | 1985 | 2983526 |
| a viral antigen-bearing cell line derived from culture of paget's bone cells. | this study documents the characteristics of a viral antigen-bearing cell line derived from co-culture of the bone from a 71-year-old woman with paget's disease of bone and hep-2 cells. the cell line has survived in continuous culture for 3 1/2 years and 185 subcultures. the cells are epithelioid in appearance, produce alkaline and acid phosphatase, increase alkaline phosphatase activity in response to 1,25-(oh)2-d3 and contain receptors for 1,25-(oh)2-d3. immunofluorescent studies utilizing anti ... | 1985 | 2996574 |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| serum antibody to respiratory syncytial virus in goats in the uk. | 1985 | 2988182 | |
| persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83. | virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ... | 1985 | 3000066 |
| production of monoclonal antibodies against parainfluenza 3 virus and their use in diagnosis by immunofluorescence. | monoclonal antibodies were produced against parainfluenza virus type 3 (pi-3) and used to identify pi-3 clinical isolates in cell culture and pi-3 antigen in cells obtained from nasopharyngeal (np) washes of patients. two (2e9 and 4g5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton pi-3 protein, and one antibody (4e5) reacted with two viral proteins in the range of 29,000 to 31,000 daltons. the three monoclonal antibodies did not cross-react by in ... | 1985 | 3001131 |
| [method of spread of respiratory syncytial virus infection in a neonatology unit]. | 1985 | 3157115 | |
| the effects of acute respiratory virus infection upon tracheal mucous transport. | tracheal mucous velocity was measured in 13 healthy non-smokers using a radioisotope-labeled aerosol and a multidetector probe during respiratory virus infections. the movement of boluses of tracheal mucous were either absent or reduced in number in five subjects with myxovirus infection (four influenza and one respiratory syncytial virus) within 48 hr of the onset of symptoms and in four subjects 1 wk later. one subject with influenza still had reduced bolus formation 12-16 wk after infection. ... | 1985 | 3002287 |
| a survey of respiratory syncytial virus and parainfluenza virus type 3 neutralising and immunoprecipitating antibodies in relation to paget disease. | the aetiology of paget disease of bone has not been established but certain features have suggested involvement of a parainfluenzalike virus. to seek further evidence of the possible role of paramyxoviruses in paget disease we have surveyed the presence of neutralising and immunoprecipitating antibodies to both respiratory syncytial virus and parainfluenza virus type 3 in the sera of patients attending a bone disease clinic. these two viruses were implicated by the sporadic observation of viral ... | 1985 | 3001223 |
| clinical applications of antiviral agents for chemophrophylaxis and therapy of respiratory viral infections. | table iii summarizes clinical applications of antiviral agents in respiratory viral infections. (table: see text) for influenza a virus infections, both oral amantadine and rimantadine are effective when used for seasonal prophylaxis and for prophylaxis in institutional populations. both of these drugs, as well as aerosolized ribavirin, have antiviral and therapeutic effects in uncomplicated influenza. it remains to be determined whether any of these modalities or possibly their combined use [44 ... | 1985 | 2417551 |
| [respiratory syncytial virus infection, with special reference to recent findings]. | 1985 | 2422814 | |
| two distinct subtypes of human respiratory syncytial virus. | antigenic variation of human respiratory syncytial (rs) virus strains was analysed using a collection of nine, six, six, nine and one monoclonal antibodies respectively directed against the large glycoprotein (g), fusion protein (f), matrix protein (m), nucleoprotein (np) and phosphoprotein (p) components of the long strain of rs virus. a comparison was made with seven other strains isolated during different years in radioimmune precipitation analyses and immune fluorescence tests. two different ... | 1985 | 2413163 |
| bovine respiratory syncytial virus infection in an ontario cattle herd. | bovine respiratory syncytial virus was recovered from the lung of a six month old calf that died during an outbreak of respiratory disease in a cattle herd in ontario. lung tissue removed from the calf at necropsy, performed within two hours of death, was frozen at -70 degrees c prior to virus isolation attempts. syncytia and intracytoplasmic inclusions were demonstrated both in histological sections of the calf's lung and in stained cell culture preparations infected with the bovine respiratory ... | 1985 | 17422509 |
| serological evidence of respiratory syncytial virus infection in lambs. | 1985 | 16031205 | |
| long-term prospective study in children after respiratory syncytial virus infection. | we have prospectively evaluated for the past 8 years 29 children who were hospitalized during infancy with acute lower respiratory tract illness caused by respiratory syncytial virus (rsv). no differences in the prevalence of a family history of atopy or breast-feeding in these infants compared with controls were noted. however, a history of parental smoking was significantly associated with hospital admission for rsv lower respiratory tract disease. evidence of atopy, as defined by serum ige le ... | 1984 | 6470859 |
| nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. | the amino acid sequence of the matrix protein of the human respiratory syncytial virus (rs virus) was deduced from the sequence of a cdna insert in a recombinant plasmid harboring an almost full-length copy of this gene. it specifically hybridized to a single 1,050-base mrna from infected cells. the recombinant containing 944 base pairs of rs viral matrix protein gene sequence lacked five nucleotides corresponding to the 5' end of the mrna. the nucleotide sequence of the 5' end of the mrna was d ... | 1984 | 6699948 |
| field trials of a bovine respiratory syncytial virus vaccine. | a vaccine against bovine respiratory syncytial virus (brsv: norden), a cause of pulmonary edema-emphysema and pneumonia in calves, was evaluated in field trials on western nebraska beef calves at branding, preweaning, weaning and postweaning. of calves with undifferentiated respiratory infection at weaning, 20.9% of 2-dose vaccinates (169/810) and 27.5% of 1-dose vaccinates (230/837) required treatment, vs 48.2% of unvaccinated controls (397/823). at postweaning, 2.4% of 2-dose vaccinates (13/54 ... | 1984 | 6493205 |
| [viral-microbial associations and the function of humoral factors of natural immunity in acute pneumonia patients]. | a total of 359 patients with acute pneumonia and 152 practically healthy subjects comprising the control group were examined. immunofluorescence was used to investigate nasopharyngeal washings for detecting antigens of influenza and parainfluenza viruses, respiratory-syncytial virus, adenoviruses, whereas serological studies according to the hemagglutination delay test with diagnostic agents for detecting influenza a1, a2, b, types 1, 2 and 3 parainfluenza, and the complement fixation test were ... | 1984 | 6523359 |
| propagation of bovine respiratory syncytial virus in calf testicle microcarrier culture. | 1984 | 6543300 | |
| [exanthematous viral diseases and measles: serology]. | a study of antibodies against measles virus and other viral agents is made to investigate possible existence of a false measles or "measles-like" in 48 children with this disease during an epidemic. hemagglutination-inhibiting technique was used to determine rubella antibodies, and complement fixation technique to measure those produced against other diseases studied. results were grouped according to the seroconversions found. the following serologic diagnoses were found: twenty eight cases of ... | 1984 | 6703539 |
| immune adherence hemagglutination test for detection of antibody to respiratory syncytial virus. brief report. | an immune adherence hemagglutination test was established for detection of antibodies to respiratory syncytial virus. the test was shown to be as specific as and much more sensitive than the neutralization and complement fixation tests. | 1984 | 6497660 |
| prevalence of antibodies to respiratory syncytial virus in various populations in israel. | a preliminary study on the prevalence of antibodies to respiratory syncytial virus (rsv) in individuals of various age-groups was carried out. in healthy infants aged 6 to 12 months, the prevalence was 27%. it increased to 37 and 54% in children aged 1 to 2 and 2 to 4 years, respectively. the prevalence reached 78% in healthy individuals who were more than 20 years old, including pregnant women. significant levels of specific antibodies were detected in 59% of children aged 6 to 12 months who we ... | 1984 | 6519945 |
| an outbreak of respiratory syncytial virus infection in an old people's home. | at least 20 of the 50 residents of an old people's home suffered from an acute respiratory infection and four of them died. respiratory syncytial virus was isolated from two of the patients. the importance of this virus in the elderly is probably underestimated. | 1984 | 6736667 |
| some respiratory and enteric diseases of cattle: an update. | fibrinous pneumonia caused 40-45% of death losses in cattle shipped into bruce county, ontario. feeding corn silage within the first 2 weeks of arrival increased mortality. commingling cattle from different sources and keeping more than 110 cattle in a pen were detrimental. while use of antimicrobials in the starter ration was beneficial, prophylactic use of antimicrobials in the water was not. mortality was directly proportional to morbidity and treatment costs. vaccinating against any respirat ... | 1984 | 6738491 |
| outbreak of respiratory syncytial virus infection in a neonatal intensive care unit. | 1984 | 6519955 | |
| the development of respiratory syncytial virus-specific immune complexes in nasopharyngeal secretions following natural infection. | sequential samples of nasopharyngeal secretions (nps) from 61 infants and children with respiratory syncytial virus (rsv) infection were examined for the presence of rsv-specific immune complexes in order to determine their possible role in recovery from or pathogenesis of rsv infection. immune complexes in nps were identified by the raji cell assay using indirect immunofluorescence and rsv antigen in these complexes was also detected by indirect immunofluorescence. rsv-specific immune complexes ... | 1984 | 6527374 |
| sequence analysis of the respiratory syncytial virus phosphoprotein gene. | a recombinant cdna plasmid (prsa3) containing an almost full-length copy of the mrna encoding respiratory syncytial virus phosphoprotein was identified in a cdna library prepared with mrna from respiratory syncytial virus-infected cells. the cdna insert was sequenced, and a protein of 27,150 daltons was deduced from the dna sequence. the protein is relatively acidic, containing two clusters of acidic amino acids, one in the middle of the molecule and the other at the c-terminus. it is devoid of ... | 1984 | 6548527 |
| respiratory virus infection in hospitalized children in austria 1979-1982. diagnosis by immunofluorescence. | nasal secretions from 349 austrian children under six years of age who were hospitalized for respiratory illnesses were screened for the presence of respiratory syncytial virus (rsv), parainfluenza virus 1 and 3, adenovirus and influenza a virus over a period of four years by the immunofluorescence technique. 35% of the specimens were found to be positive for one of the five viruses investigated. rsv was detected in 31% of the nasal secretions and was thus the most frequently encountered causati ... | 1984 | 6381315 |
| pathogenesis of experimental bovine respiratory syncytial virus infection in sheep. | conventionally reared lambs, seronegative to bovine respiratory syncytial virus (rsv), were inoculated with bovine rsv by an aerosol route. a mild clinical response, characterized by transient fever at postinoculation day (pid) 4, occurred and was accompanied by serous oculonasal secretions in less than 50% of the infected lambs. a decrease in total wbc was observed on pid 6 and 11, with a reduction in the total numbers of lymphocytes. bovine rsv was recovered from nasal secretions of inoculated ... | 1984 | 6383151 |
| interaction of bovine respiratory syncytial virus and pasteurella haemolytica in the ovine lung. | the potential synergistic effect of bovine respiratory syncytial virus (rsv) and pasteurella haemolytica in the production of pneumonia after aerosol/intranasal infection of conventionally reared lambs was evaluated. a mild clinical response was observed in lambs given virus and/or bacteria. gross pulmonary lesions were seen in 3 of 6 lambs given rsv and then p haemolytica 3 or 6 days later, respectively (groups d and e), and in 1 lamb of 5 given virus and bacteria simultaneously (group g). gros ... | 1984 | 6383152 |
| immune response to respiratory syncytial virus: prevention of syncytia formation by human serum during in vitro infection. | human serum specimens containing respiratory syncytial virus (rsv)-specific neutralizing antibody were found to prevent the formation of syncytia when applied to hep-2 tissue culture monolayers which had been infected with rsv 12 h previously. this was evidenced by the demonstration of rsv-infected cells without any syncytia formation in the monolayers treated with rsv antibody-positive serum. on the other hand, widespread syncytia formation was observed with antibody-negative control serum. the ... | 1984 | 6387611 |
| isolation of pathogenic bacteria from the nasopharynx of children with respiratory syncytial virus infection. predictive value of chest roentgen examination and laboratory tests. | potentially pathogenic bacteria were isolated from the nasopharynx in 33/66 hospitalized infants and children with verified respiratory syncytial virus (rsv) infection. the value of chest roentgenograms and blood counts for the prediction of concomitant bacterial infection was evaluated. abnormal chest roentgenograms were found in 89% whether pathogenic bacteria were present or not. the most common finding was general hyperinflation of the lungs, with or without infiltrates. this occurred more f ... | 1984 | 6377479 |
| giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome. | a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ... | 1984 | 6611144 |
| an enzyme-linked immunosorbent assay (elisa) for igg and iga antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions. | an enzyme-linked immunosorbent assay (elisa) has been developed for titration of igg and iga antibodies to respiratory syncytial (rs) virus in low dilutions of human serum, colostrum, and nasopharyngeal secretions. previously the sensitivity of rs virus elisa on such specimens has been limited by nonspecific absorption of antibody, particularly iga, to crude antigen preparations. for igg antibody estimation in infant sera, this unwanted binding was reduced to workable levels by increasing the se ... | 1984 | 6389773 |
| antibiotic treatment of pneumonia and bronchiolitis. a prospective randomised study. | routine administration of antibiotics in the treatment of pneumonia and bronchiolitis in infants and small children was evaluated in an open randomised prospective trial. from 1979-82 136 children between the age of 1 month and 6 years were allocated to one of two treatment groups shortly after their admission to a paediatric ward. group a patients were to be given antibiotics but those in group b were not. none of the children had received antibiotics before hospital admission. a viral infectio ... | 1984 | 6391389 |
| respiratory viruses and sudden infant death. | viruses were shown to be present in the respiratory tract in 200 of 763 cases of the sudden infant death syndrome studied in the nine years 1974-82. epidemiological and pathological evidence suggested that the distribution of viruses in the sudden infant death syndrome differs between infants aged 3 months or less and those aged over 3 months: the incidence of detection of virus was 14% in the younger group compared with 39% in the older group. the distribution of the viruses in these two groups ... | 1984 | 6426615 |
| management of respiratory failure in infants with acute viral bronchiolitis. | fifteen infants with acute viral bronchiolitis required mechanical ventilation. infants were all aged less than 12 weeks and all had evidence of atelectasis or pneumonia on chest x-ray films. respiratory syncytial virus was identified by immunofluorescence in 14 of 15 patients. intubation and mechanical ventilation were initiated to reduce the work of breathing rather than to treat hypoxemia alone. additional therapy included continuous positive airway pressure or positive end-expiratory pressur ... | 1984 | 6437212 |
| identification of a tenth mrna of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. | nine mrnas, their cdna clones, and a genome transcriptional map have been reported previously for respiratory syncytial virus (p. l. collins and g. w. wertz, proc. natl. acad. sci. u.s.a. 80:3208-3212, 1983). we report here the identification of a 10th viral mrna, designated mrna 2b (molecular weight [mw] ca. 0.39 x 10(6)), that was detected by rna (northern) blot hybridization with cdna clones. analysis of a polycistronic readthrough transcript was used to deduce the position in the viral trans ... | 1984 | 6546401 |
| respiratory syncytial virus infection in mice. | the a2 strain of human respiratory syncytial virus replicated in the nose and lung of balb/c mice, with virus growing to higher titers in older animals than in younger animals. virus was recovered from the nose between days 2 and 7 with peak titers on days 3 and 4, and from the lungs between days 2 and 9, with peak titers on days 4 through 6. serum antibody developed 2 weeks after infection. viral antigen was demonstrated in the alveolar cells of the lung by immunofluorescence. histopathological ... | 1984 | 6693171 |
| cell-mediated immunity in respiratory syncytial virus disease. | systemic cell-mediated responses to respiratory syncytial (rs) virus were detected, using a whole blood transformation assay, in 10 of 28 infants and children with rs virus infections during the period 1-14 days postadmission. cell-mediated responses were unrelated to the age of the patient or the severity of illness. no correlation was found between cellular responses and fourfold or greater rises in antibody titre to rs virus, as determined by a membrane immunofluorescence technique. patients ... | 1984 | 6693860 |
| ribavirin. | despite the plethora of antibiotics available for the treatment of bacterial infections, very few agents have been developed to treat viral diseases. ribavirin (virazole) is a triazole nucleoside antiviral agent that produces selective antiviral effects against a broad spectrum of rna and dna viruses. the drug has been effective in the treatment of naturally occurring influenza a and b infections when administered by aerosol; oral administration has been ineffective. ribavirin aerosol therapy al ... | 1984 | 6697874 |
| pathogens associated with acute lower respiratory tract infection in young children. | to determine the agents associated with acute lower respiratory infection in young children, we studied 102 hospitalized children less than 5 years old using culture and serology for viruses and chlamydia trachomatis, fluorescent antibody testing for pertussis and respiratory syncytial virus, blood cultures and counterimmunoelectrophoresis of nasopharyngeal secretions and urine for pneumococcal and haemophilus influenzae type b antigens. at least one agent was detected in 87 children and multipl ... | 1984 | 6701101 |
| bovine respiratory syncytial virus infections in young dairy cattle: clinical and haematological findings. | clinical signs and haematological findings during the acute and convalescent phases of an infection with bovine respiratory syncytial virus (rsv) were studied under field conditions. the study was carried out in 139 cattle less than 16 months of age in 16 herds with serologically proven bovine rsv infections. blood was collected for serological and haematological examination. repeated clinical examinations were carried out until 35 days after appearance of disease. signs of general disease such ... | 1984 | 6702077 |
| [isolation of the respiratory syncytial virus from calves with respiratory diseases]. | described is a respiratory enzootic in calves aged 30 to 90 days. two cytopathic strains of the bovine respiratory syncytial virus were isolated from diseased calves in a cell culture of lamb thyroid. clinical observations and the results of virologic and serologic investigations have revealed that mixed infections of the respiratory syncytial virus and the mucosa disease virus take part in the etiology of the respiratory enzootic described. | 1984 | 6730327 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |