Publications

the role of gammadelta t cells in induction of bacterial antigen-specific protective cd8+ cytotoxic t cells in immune response against the intracellular bacteria listeria monocytogenes.	the role of t-cell receptor (tcr) gammadelta t cells in the induction of protective tcr alphabeta t cells against infection by the intracellular bacteria listeria monocytogenes was analysed. we found that depletion of gammadelta t cells by anti-tcr delta monoclonal antibody treatment before intravenous immunization of mice with a sublethal dose of viable l. monocytogenes resulted in reduction of protection against secondary challenge infection in the immunized mice. the gammadelta t-cell depleti ...	1998	9824480
gamma delta t cells in infection-induced and autoimmune-induced testicular inflammation.	in a previous report, we investigated inflammatory responses induced by injecting listeria monocytogenes into one testis of a mouse. we demonstrated that the contralateral testis also developed an orchitis despite the absence of bacteria, indicating that the inflammation on the uninfected, contralateral side was of autoimmune character. in both infected and autoimmune testes, gammadelta and alphabeta t cells infiltrated during the inflammation. in this paper, we present the data of a comparison ...	1998	9824503
interleukin-15 may be responsible for early activation of intestinal intraepithelial lymphocytes after oral infection with listeria monocytogenes in rats.	exogenous interleukin-15 (il-15) stimulates intestinal intraepithelial lymphocytes (i-iel) from mice to proliferate and produce gamma interferon (ifn-gamma) in vitro. to determine whether endogenous il-15 is involved in activation of i-iel during intestinal infection, we examined il-15 synthesis by intestinal epithelial cells (i-ec) after infection with listeria monocytogenes in rats. in in vitro experiments, invasion of l. monocytogenes into iec-6 cells, a rat small intestine epithelial cell li ...	1998	9826341
phosphatidylcholine-specific phospholipase c from listeria monocytogenes is an important virulence factor in murine cerebral listeriosis.	meningoencephalitis is a serious and often fatal complication of listeria monocytogenes infection. the aim of the present study was to analyze the role of internalin a (inla) and b, which are involved in the invasion of l. monocytogenes into cultivated host tissue cells, and that of phosphatidylcholine-specific phospholipase c (plcb), which mainly promotes the direct cell-to-cell spread of l. monocytogenes, in murine cerebral listeriosis by use of an inla/b (deltainlab2)- and a plcb (deltaplcb2) ...	1998	9826375
organic acid dipping of catfish fillets: effect on color, microbial load, and listeria monocytogenes.	microbiological and color changes of catfish fillets were determined following dip treatment in solutions at 4 degrees c of 2% acetic, citric, hydrochloric, lactic, malic, or tartaric acid. fillets were inoculated with an eight-strain mixture of listeria monocytogenes prior to dipping. l. monocytogenes, coliform, and aerobic plate counts and surface ph and hunter color were measured at 0, 2, 5, and 8 days of storage at 4 degrees c. acid dipping reduced surface ph and l. monocytogenes, coliform, ...	1998	9829187
behavior of listeria spp. in smoked fish products affected by liquid smoke, nacl concentration, and temperature.	the growth, survival, and death of listeria monocytogenes were studied in a synthetic medium as a function of temperature, nacl content, and amount of liquid smoke, and the findings were validated in smoked fish products. the smoke preservative compound was simulated by adding liquid smoke, and the concentration was expressed as phenol concentration. the growth of l. monocytogenes was limited at a temperature as low as 4 degrees c or at a phenol concentration as high as 20 ppm. the predicted val ...	1998	9829188
tolerance limits and methodology: effect on international trade.	microbiological contamination of foods with listeria monocytogenes, salmonella spp., campylobacter spp., and other pathogens and toxins and chemical and environmental contaminants can cause serious health and trade problems in the international trade of foods. consequently, a system of monitoring and surveillance of the quality and safety of imported foods can have a significant impact on food trade between two or more countries. the world trade organization (wto) provides a framework for ensuri ...	1998	9829206
a homolog of ccpa mediates catabolite control in listeria monocytogenes but not carbon source regulation of virulence genes.	readily utilizable sugars down-regulate virulence gene expression in listeria monocytogenes, which has led to the proposal that this regulation may be an aspect of global catabolite regulation (cr). we recently demonstrated that the metabolic enzyme alpha-glucosidase is under cr in l. monocytogenes. here, we report the cloning and characterization from l. monocytogenes of an apparent ortholog of ccpa, which encodes an important mediator of cr in several low-g+c-content gram-positive bacteria. l. ...	1998	9829942
variations in tolerance of listeria monocytogenes to nisin, pediocin pa-1 and bavaricin a.	a collection of 381 listeria monocytogenes strains was examined for strain variations in nisin and pediocin sensitivity at three different concentrations on tryptic soya agar. two of the strains were able to grow on agar plates containing 500 iu nisin ml-1. these strains were characterized as having an enhanced nisin tolerance. twenty strains had normal growth on agar plates containing 1600 au pediocin ml-1 and higher. these strains were characterized as pediocin-resistant. another 34 strains, c ...	1998	9830144
[listeria monocytogenes rhomboencephalitis. clinical case].	we report a previously healthy 44 years old female, that presented with mild clouding of consciousness, a left cerebellar syndrome, involvement of v, x and xii left cranial nerves and an alteration of epicritic sensitivity in the left half of the body. cerebrospinal fluid had inflammatory features. cerebrospinal fluid and blood cultures were positive for listeria monocytogenes. magnetic resonance imaging disclosed a rhomboencephalitis. antibiotics were started and the clinical condition of the p ...	1998	9830776
in vitro activities of ketolide hmr 3647, macrolides, and clindamycin against coryneform bacteria.	the in vitro activity of ketolide hmr 3647 against coryneform bacteria isolated from clinical samples was evaluated. except against corynebacterium jeikeium and c. urealyticum, hmr 3647 showed high activity against corynebacterium spp., being more active than 14- and 16-membered macrolides, azithromycin, or clindamycin. hmr 3647 also had high in vitro activity against brevibacterium spp. and listeria monocytogenes.	1998	9835530
use of a genetically enhanced, pediocin-producing starter culture, lactococcus lactis subsp. lactis mm217, to control listeria monocytogenes in cheddar cheese	cheddar cheese was prepared with lactococcus lactis subsp. lactis mm217, a starter culture which contains pmc117 coding for pediocin pa-1. about 75 liters of pasteurized milk (containing ca. 3.6% fat) was inoculated with strain mm217 (ca. 10(6) cfu per ml) and a mixture of three listeria monocytogenes strains (ca. 10(3) cfu per ml). the viability of the pathogen and the activity of pediocin in the cheese were monitored at appropriate intervals throughout the manufacturing process and during ripe ...	1998	9835572
purification and genetic characterization of enterocin i from enterococcus faecium 6t1a, a novel antilisterial plasmid-encoded bacteriocin which does not belong to the pediocin family of bacteriocins.	enterocin i (enti) is a novel bacteriocin produced by enterococcus faecium 6t1a, a strain originally isolated from a spanish-style green olive fermentation. the bacteriocin is active against many olive spoilage and food-borne gram-positive pathogenic bacteria, including clostridia, propionibacteria, and listeria monocytogenes. enti was purified to homogeneity by ammonium sulfate precipitation, binding to an sp-sepharose fast-flow column, and phenyl-sepharose cl-4b and c2/c18 reverse-phase chroma ...	1998	9835578
evaluation of universal preenrichment broth for the recovery of foodborne pathogens from milk and cheese.	the use of universal preenrichment broth for the recovery of verotoxigenic escherichia coli, salmonella spp., and listeria monocytogenes from milk and cheese was examined. universal preenrichment broth supported the growth of low inoculum levels (10 cfu/ml) of these organisms in pure cultures and in mixed cultures containing higher levels of other pathogens or bacterial flora from raw milk. this medium also supported the recovery and growth of heat-injured salmonella spp., l. monocytogenes, and ...	1998	9839221
antimicrobial effect of enterocin ccm 4231 in the cattle slurry environment.	the antagonistic effect of enterocin ccm 4231 towards enterococci, staphylococci, escherichia coli, listeriae and pseudomonads in the cattle slurry environment was assessed during periods of 1 and 2 weeks. the maximum decrease in the viable cells of enterococci and staphylococci (5.39 to 1.1 log cfu ml-1, and 4.3 to 2.3 log cfu ml-1, respectively) was detected on the second day after enterocin ccm 4231 addition to cattle slurry. e. coli cells, listeriae and pseudomonads decreased insignificantly ...	1998	9839432
effect of feline immunodeficiency virus on cytokine response to listeria monocytogenes in vivo.	feline immunodeficiency virus (fiv) is a lentivirus that induces an acquired immunodeficiency in domestic cats. the objective of this study was to compare the immune response of chronically fiv-infected cats and specific pathogen free (spf) cats to listeria monocytogenes, a facultative intracellular bacterium. regional lymph nodes were removed at various times after subcutaneous inoculation with l. monocytogenes and evaluated. lymph nodes of chronically fiv-infected cats enlarged more slowly and ...	1998	9839868
the suitability and application of a gfp-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells.	the product of a gfp-actin gene fusion, permanently or transiently transfected in diverse mammalian cell lines, was shown to be a suitable, intrinsic probe of both the organization and dynamics of the actin cytoskeleton. in live swiss 3t3 and nih 3t3 cells, the fusion protein was found to accumulate in lamellipodia, filopodia, focal contacts and stress fibers. furthermore, comparisons of fluorescence images of gfp-actin and cy3.5-phalloidin, an independent marker of f-actin, in permeabilized cel ...	1998	9840457
listeria monocytogenes causing solitary liver abscess. case report and review of the literature.	the authors report on a case of a solitary liver abscess due to listeria monocytogenes in a 53-year-old diabetic white male and review all published cases of solitary listerial abscesses of the liver. l. monocytogenes is a rare cause of solitary liver abscess which occurs in elderly patients with diabetes mellitus. the clinical signs are variable and often mimic malignancy, with epigastric pain, night sweats and weight loss. prevalent features are poor control of glycemia, temperature up to 38.5 ...	1998	9845615
the effect of the growth environment on the lag phase of listeria monocytogenes.	the duration of lag in listeria monocytogenes was examined in relation to the physico-chemical properties of the growth environment. it was supposed that lag would be determined by two hypothetical quantities, the amount of work that a cell has to perform to adapt to new conditions and the rate at which it can perform that work. if the rate at which the cell can perform the necessary work is a function of the maximum specific growth rate in the new environment, the hypothesis predicts that lag t ...	1998	9849786
initial numbers, serovars and phagevars of listeria monocytogenes isolated in prepared foods in the city of barcelona (spain).	a total of 1100 samples of prepared foods purchased in restaurants and delicatessen shops in the city of barcelona was examined for the presence of listerio. l. monocytogenes was more frequently isolated from foods intended to be consumed without further cooking (9.3%) than from foods intended to receive further cooking prior to consumption (2.9%). a quantitative study, carried out with 773 samples, yielded 1% of samples with numbers of l. monocytogenes higher than 100 cfu/g. strains of l. monoc ...	1998	9849792
irradiation as a method for decontaminating food. a review.	despite substantial efforts in avoidance of contamination, an upward trend in the number of outbreaks of foodborne illnesses caused by nonsporeforming pathogenic bacteria are reported in many countries. good hygienic practices can reduce the level of contamination but the most important pathogens cannot presently be eliminated from most farms nor is it possible to eliminate them by primary processing, particularly from those foods which are sold raw. several decontamination methods exist but the ...	1998	9851599
functional similarities between the listeria monocytogenes virulence regulator prfa and cyclic amp receptor protein: the prfa* (gly145ser) mutation increases binding affinity for target dna.	most listeria monocytogenes virulence genes are positively regulated by the prfa protein, a transcription factor sharing sequence similarities with cyclic amp (camp) receptor protein (crp). its coding gene, prfa, is regulated by prfa itself via an autoregulatory loop mediated by the upstream prfa-dependent plca promoter. we have recently characterized prfa* mutants from l. monocytogenes which, as a result of a single amino acid substitution in prfa, gly145ser, constitutively overexpress prfa and ...	1998	9852011
nisin resistance in listeria monocytogenes atcc 700302 is a complex phenotype.	nisin resistance in listeria monocytogenes atcc 700302 is a complex phenotype involving alterations in both the cytoplasmic membrane and the cell wall and a requirement for divalent cations. in addition to a lower ratio of c15 to c17 fatty acids than in the wild-type strain (a. s. mazzotta and t.j. montville, j. appl. microbiol. 82: 32-38, 1997), this nisin-resistant (nisr) strain contained significantly more zwitterionic phosphatidylethanolamine and less anionic phosphatidylglycerol and cardiol ...	1998	9435079
risk assessment for the harmful effects of uvb radiation on the immunological resistance to infectious diseases.	risk assessment comprises four steps: hazard identification, dose-response assessment, exposure assessment, and risk characterization. in this study, the effects of increased ultraviolet b(uvb, 280-315 nm) radiation on immune functions and the immunological resistance to infectious diseases in rats were analyzed according to this strategy. in a parallelogram approach, nonthreshold mathematical methods were used to estimate the risk for the human population after increased exposure to uvb radiati ...	1998	9435148
essential roles for granulocyte-macrophage colony-stimulating factor (gm-csf) and g-csf in the sustained hematopoietic response of listeria monocytogenes-infected mice.	the in vivo roles of granulocyte-macrophage colony-stimulating factor (gm-csf) and granulocyte (g)-csf were studied in factor-deficient gene-targeted knockout mice infected with the facultative intracellular bacterium listeria monocytogenes. previous results showed that g-csf-/- mice had an underlying selective deficiency in granulopoiesis, but gm-csf-/- mice had little disturbance in resting hematopoiesis. nevertheless, in this study it is revealed that 3 days after intraperitoneal infection wi ...	1998	9446646
interleukin-8 release from human neutrophils after phagocytosis of listeria monocytogenes and yersinia enterocolitica.	the release of interleukin-8 (il-8) from human neutrophils was investigated after their internalisation of the invasive bacteria listeria monocytogenes and yersinia enterocolitica. three pairs of bacterial strains were used to study the influence of listeriolysin o, invasiveness and yersinia virulence plasmid (pyv)-encoded proteins on neutrophil cytokine synthesis. the neutrophils secreted il-8 into the culture supernate after exposure to all strains tested. with l. monocytogenes, the virulence ...	1998	9449950
role of gamma delta t cells in immunity to infectious diseases and the regulation of hematolymphoid cell development.	my research interests are twofold. the first is to define the biochemical and molecular mechanisms that regulate hematopoietic cell development. in particular, the role that the cytokine interleukin-2 (il2) plays in regulating the development and selection of lymphocyte progenitor cells, and in myelopoiesis are primary areas of research. the second is to understand the role that gamma delta t cells play in pathogen-induced immune responses and autoimmunity. their involvement in the immune respon ...	1998	9479563
host cell signalling during listeria monocytogenes infection.	macrophages and other mammalian cells respond to infection with listeria monocytogenes by the transient or persistent activation of host cell signal transduction pathways. in addition, l. monocytogenes infection influences expression of various host cell genes, some of which may hinder or favour bacterial replication. the observed host cell responses vary with the different subcellular locations inhabited by l. monocytogenes during its intracellular life cycle.	1998	9481817
control of actin dynamics.	actin-based motility processes are tightly linked to the rapid turnover of actin filaments. factors that control the steady state of actin assembly, such as capping proteins and actin-depolymerizing factor/cofilin, directly affect motility. actin-depolymerizing factor increases the treadmilling of actin filaments in vitro and in vivo. cellular factors that are involved in linking initiation of barbed end assembly to cell signaling are being identified using listeria monocytogenes and saccharomyc ...	1998	9484594
bacterial delivery of dna evolves.		1998	9487514
delivery of antigen-encoding plasmid dna into the cytosol of macrophages by attenuated suicide listeria monocytogenes.	eukaryotic expression vectors can be delivered to macrophages using attenuated self-destructing listeria monocytogenes. l. monocytogenes cells are preferentially lysed in the host cell macrophage cytosol by the production of a pacta-dependent listeria-specific phage lysin. efficient expression of the cloned reporter genes by the macrophages and subsequent antigen presentation were achieved after the delivery of eukaryotic expression vectors by the attenuated suicide l. monocytogenes strain. afte ...	1998	9487527
h2m3wt-restricted, listeria monocytogenes-immune cd8 t cells respond to multiple formylated peptides and to a variety of gram-positive and gram-negative bacteria.	a subset of h2m3wt-restricted, listeria monocytogenes (lm)-immune cd8 effectors recognize antigen-presenting cells (apc) preincubated with heat-killed lm. the responsible product, which we have previously designated heat-killed listeria-associated antigen (haa), is extremely hydrophobic and resistant to proteolytic degradation. despite the protease resistance of haa, we now report that haa-immune clones are uniformly responsive to fmigwii, a formylated oligopeptide derived from the recently desc ...	1998	9488151
listeria monocytogenes invasion of epithelial cells requires the mek-1/erk-2 mitogen-activated protein kinase pathway.	pd98059, a specific inhibitor of mek-1 mitogen-activated protein (map) kinase kinase, blocked listeria monocytogenes invasion into hela epithelial cells. the effects of pd98059 were reversible, as adherent extracellular bacteria were internalized upon removal of the drug. previously, we reported that l. monocytogenes could activate erk-1 and erk-2 map kinases through the action of listeriolysin o (llo) on the host cell (p. tang, i. rosenshine, p. cossart, and b. b. finlay, infect. immun. 64:2359 ...	1998	9488402
fitness costs associated with class iia bacteriocin resistance in listeria monocytogenes b73.	in order to assess the potential for the spread of class iia bacteriocin resistance in natural populations of listeria monocytogenes, the fitness costs associated with resistance to leucocins a, b and e and sakacin a in l. monocytogenes b73 in the absence of bacteriocin were examined. the resistant phenotype had a lower growth rate (and thus relative fitness) than the sensitive phenotype in monoculture experiments. furthermore, resistant phenotypes were unable to invade populations of the sensit ...	1998	9489026
separation of listeria monocytogenes and salmonella berta from a complex food matrix by aqueous polymer two-phase partitioning.	in the present study, the use of aqueous polymer two-phase systems for separation of pathogenic bacteria from a complex food sample was investigated. three different two-phase systems, a polyethylene glycol 3350/dextran t 500, a methoxy polyethylene glycol 5000/dextran t 500 and a polyethylene glycol 3350/hydroxypropyl starch system, were compared at ph 3 and ph 6 for their capacity to separate the pathogenic bacteria listeria monocytogenes and salmonella berta from a cumberland sausage. in all ...	1998	9489033
sequence comparison of the chromosomal regions encompassing the internalin c genes (inlc) of listeria monocytogenes and l. ivanovii.	we have recently cloned and characterized the inlc gene of listeria monocytogenes which belongs to the listerial internalin multigene family and codes for a 30-kda secreted protein containing five consecutive leucine-rich repeats. here, we show that in l. monocytogenes inlc is located between the rpls gene (encoding the 50s ribosomal protein l19), and the infc gene (encoding the translation initiation factor 3). by direct and inverse polymerase chain reactions (pcr), we cloned a 5.4-kb region co ...	1998	9491077
compartmentalization of bacterial antigens: differential effects on priming of cd8 t cells and protective immunity.	bacterial pathogens synthesize numerous proteins that are either secreted or localized within bacterial cells. to address the impact of antigen compartmentalization on t cell immunity, we constructed recombinant listeria monocytogenes that express a model cd8t cell epitope as a secreted or nonsecreted fusion protein. both forms of the antigen, either secreted into the host cell cytoplasm or retained within bacterial cells, efficiently prime cd8 t cell responses. however, epitope-specific cd8 t c ...	1998	9491894
the microtubule depolymerizing drugs nocodazole and colchicine inhibit the uptake of listeria monocytogenes by p388d1 macrophages.	uptake of listeria monocytogenes by different mammalian cells like macrophages and epithelial cells is dependent on functional actin filaments and hence susceptible to inhibition by cytochalasin. here we show that phagocytic uptake of l. monocytogenes by p388d1 macrophages is also highly sensitive to treatment with the microtubule depolymerizing drugs nocodazole and colchicine. this sensitivity is cell type specific and much less pronounced in bone marrow-derived macrophages and caco-2 epithelia ...	1998	9495017
in situ detection of a virulence factor mrna and 16s rrna in listeria monocytogenes.	simultaneous in situ analysis of the structure and function of bacterial cells present within complex communities is a key for improving our understanding of microbial ecology. a protocol for the in situ identification of listeria spp. using fluorescently tagged, rrna-targeted oligonucleotide probes was developed. ethanol fixation and enzymatic pretreatment with lysozyme and proteinase k were used to optimize whole cell hybridization of exponential phase and stationary phase listeria spp. cells. ...	1998	9495027
classification of listeria monocytogenes by pcr-restriction enzyme analysis in the two genes of hlya and iap.	pcr-restriction enzyme analysis in the two virulence-associated genes was performed. the hlya gene cording for listeriolysin o and the iap gene cording for an invasion associated factor were amplified with primers sh2 or si3. the pcr products obtained were cleaved with 32 restriction enzymes, and restriction profiles from 12 strains, 6 each of serotypes 1/2a and 4b, were compared. we obtained two profiles for the hlya using 4 restriction enzymes and eight profiles for the iap by using alui, and ...	1998	9497934
evidence for expressional crosstalk between the central virulence regulator prfa and the stress response mediator clpc in listeria monocytogenes.	virulence is a multifactorial trait which depends on the coordinated expression of many bacterial products, hence it is to be expected that the regulatory circuits that control the relevant genetic determinants are somehow interconnected. two pleiotropic regulatory elements acting at different levels, the transcription factor prfa which controls virulence gene expression and the potential chaperone clpc which is involved in tolerance to environmental stress, are required for listeria monocytogen ...	1998	9453154
the role of sialic acid in opsonin-dependent and opsonin-independent adhesion of listeria monocytogenes to murine peritoneal macrophages.	the adhesion of listeriae to host cells employs mechanisms which are complex and not well understood. listeria monocytogenes is a facultative intracellular pathogen responsible for meningoencephalitis, septicemia, and abortion in susceptible and immunocompromised individuals. subsequent to colonization and penetration of the gut epithelium, the organism attaches to resident macrophages and replicates intracellularly, thus evading the humoral immune system of the infected host. the focus of these ...	1998	9453618
comprehensive study of the intestinal stage of listeriosis in a rat ligated ileal loop system.	the intestinal stage of listeriosis was studied in a rat ligated ileal loop system. listeria monocytogenes translocated to deep organs with similar efficiencies after inoculation of loops with or without peyer's patches. bacterial seeding of deep organs was demonstrated as early as 15 min after inoculation. it was dose dependent and nonspecific, as the delta inlab, the delta hly, and the delta acta l. monocytogenes mutants and the nonpathogenic species, listeria innocua, translocated similarly t ...	1998	9453636
dna vaccination exploits normal biology.		1998	9461180
lacticin 3147, a broad-spectrum bacteriocin which selectively dissipates the membrane potential.	lacticin 3147 is a broad-spectrum bacteriocin produced by lactococcus lactis subsp. lactis dpc3147 (m. p. ryan, m. c. rea, c. hill, and r. p. ross, appl. environ. microbiol. 62:612-619, 1996). partial purification of the bacteriocin by hydrophobic interaction chromatography and reverse-phase fast protein liquid chromatography revealed that two components are required for full activity. lacticin 3147 is bactericidal against l. lactis, listeria monocytogenes, and bacillus subtilis; at low concentr ...	1998	9464377
purification and characterization of anti-listeria compounds produced by geotrichum candidum.	geotrichum candidum can produce and excrete compounds that inhibit listeria monocytogenes. these were purified by ultrafiltration, centrifugal partition chromatography, thin-layer chromatography, gel filtration, and high-pressure liquid chromatography, and analyzed by liquid chromatography-mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and optical rotation. two inhibitors were identified: d-3-phenyllactic acid and d-3-indollactic acid.	1998	9464426
lectin reactivity and virulence among strains of listeria monocytogenes determined in vitro using the enterocyte-like cell line caco-2.	forty-six cultures of listeria monocytogenes (including clinical, food and collection strains) were serotyped, characterized for motility, haemolysis and phospholipase activities and tested for lectin agglutination using a four-lectin set. lectin reactivity (i.e. agglutination by one or more of the four lectins) was observed in all 12 clinical isolates, 16 of the 23 food isolates and eight of the 11 collection strains. virulence was evaluated in vitro based on strains' ability to invade the huma ...	1998	9467903
utilization of oligopeptides by listeria monocytogenes scott a.	for effective utilization of peptides, listeria monocytogenes possesses two different peptide transport systems. the first one is the previously described proton motive force (pmf)-driven di- and tripeptide transport system (a. verheul, a. hagting, m.-r. amezaga, i. r. booth, f. m. rombouts, and t. abee, appl. environ. microbiol, 61:226-233, 1995). the present results reveal that l. monocytogenes possesses an oligopeptide transport system, presumably requiring atp rather than the pmf as the driv ...	1998	9501445
peritonitis due to listeria monocytogenes in a patient receiving maintenance hemodialysis.		1998	9502491
psychrotrophic bacteria isolated from a constantly warm tropical environment.	psychrotrophic bacteria are known to occur in temperate, constantly cold, and artificially cooled environments. this is the first report of their occurrence in a constantly warm (ca. 24 degrees-35 degrees c) tropical environment. soil samples taken from two sites along the southeastern coastal zone of jamaica yielded growth of psychotrophic bacteria after 3-4 weeks of enrichment culture in 1/30 strength tryptic soy broth, 20 mg l-1 cycloheximide at 2 degrees c. growth of individual isolates at 2 ...	1998	9516542
coordinate regulation of complex t cell populations responding to bacterial infection.	bacterial infections activate complex t cell populations that differ in size and antigen specificity. we used tetramerized mhc class i molecules complexed with listeria monocytogenes-derived epitopes to characterize four distinct cd8+ t lymphocyte populations during bacterial infection. surprisingly, t cell populations differing in antigen specificity expand, contract, and enter the t cell memory compartment synchronously. because the four l. monocytogenes epitopes are presented with different e ...	1998	9529152
multiple hemopoietic defects and lymphoid hyperplasia in mice lacking the transcriptional activation domain of the c-rel protein.	the c-rel protooncogene encodes a member of the rel/nuclear factor (nf)-kappab family of transcriptional factors. to assess the role of the transcriptional activation domain of c-rel in vivo, we generated mice expressing a truncated c-rel (deltac-rel) that lacks the cooh-terminal region, but retains a functional rel homology domain. mice with an homozygous mutation in the c-rel region encoding the cooh terminus of c-rel (c-reldeltact/deltact) display marked defects in proliferative and immune fu ...	1998	9529314
listeriosis.	listeria monocytogenes is ubiquitous in nature and is part of the normal flora of the distal portion of the intestinal tract of numerous animal species. listeriosis is an emerging food borne disease that is responsible for approximately 1,700 cases of human illness each year and 650 deaths. listeria is the cause of three main disease entities in animals and humans: neural, visceral, and reproductive. clinical signs associated with the three forms are discussed along with diagnosis, therapy, prev ...	1998	9532671
the regulatory role of heat shock protein 70-reactive cd4+ t cells during rat listeriosis.	protection against infection with listeria monocytogenes depends primarily on listeria-specific t cells. we show here that cd4+ tcr alphabeta+ t cells are capable of recognizing the mycobacterial heat shock protein (hsp) 70, that appears in the peritoneal cavity of f344 rats infected i.p. with l. monocytogenes. the hsp70-reactive cd4+ t cells recognized a peptide comprising 234-252 residues as present in the 70 kda hsp of mycobacterium tuberculosis in the context of rt1.b mhc class ii molecules. ...	1998	9533439
determination of a 15437 bp nucleotide sequence around the inha gene of mycobacterium avium and similarity analysis of the products of putative orfs.	a 15437 bp region encompassing the inha locus from the mycobacterium avium chromosome was cloned and sequenced. from the sequencing data generated and the results of homology searches, the primary structure of this region was determined. this region contains four known genes (acna, fabg, inha and hemh) and two genes, inva and invb, whose products display homology with p60 invasion protein of listeria monocytogenes. six proteins encoded by putative orfs contained an rgd motif (often involved in b ...	1998	9534249
differential interaction of the transcription factor prfa and the prfa-activating factor (paf) of listeria monocytogenes with target sequences.	the interaction of the purified prfa transcription factor with the regulatory sequences located upstream of the prfa-dependent listeriolysin (hly) and internalin (inla) genes was studied in the presence and in the absence of paf (prfa-activating factor)-containing extracts. it is shown that prfa protein is able to bind, independently of additional factors, to a 109bp dna fragment including the entire hly promoter sequence with the anticipated prfa binding site ('prfa-box'). prfa alone, but not i ...	1998	9535082
the inib protein of listeria monocytogenes is sufficient to promote entry into mammalian cells.	inib is one of the two listeria monocytogenes invasion proteins required for bacterial entry into mammalian cells. entry into human epithelial cells such as caco-2 requires inia, whereas inib is needed for entry into cultured hepatocytes and some epithelial or fibroblast cell lines such as vero, hep-2 and hela cells. inib-mediated entry requires tyrosine phosphorylation, cytoskeletal rearrangements and activation of the host protein phosphoinositide (pi) 3-kinase, probably in response to engagem ...	1998	9535096
effect of granulocyte-macrophage colony-stimulating factor on the number of leucocytes and course of listeria monocytogenes infection in naive and leucocytopenic mice.	this study concerns the effect of recombinant murine granulocyte-macrophage colony-stimulating factor (gm-csf) on the number of circulating leucocytes, activation of peritoneal macrophages and proliferation of listeria monocytogenes in various organs of naive and leucocytopenic mice. mice were rendered leucocytopenic by sublethal total body irradiation or cyclophosphamide treatment. gm-csf treatment enhanced the number of granulocytes and monocytes in peripheral blood during l. monocytogenes inf ...	1998	9536121
[pustular dermatitis in veterinarians following delivery in farm animals; an occupational disease].	to assess the prevalence of contact dermatitis after deliveries in cattle or sheep among veterinarians.	1998	9537088
flow cytometry demonstrates bacteriocin-induced injury to listeria monocytogenes.	flow cytometry was used to study the effect of the bacteriocin leucocin b-ta11a on listeria (l.) monocytogenes. mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of the dead/live baclight bacterial viability kittm. high correlations for flow cytometric detection of defined proportions of live or dead cells in mixtures between 10 and 100% of dead (r2 = 0.97) or live (r2 = 0.99) cells were obtained. however, mixtures containing les ...	1998	9541561
identification and characterization of nucleotide sequence differences in three virulence-associated genes of listeria monocytogenes strains representing clinically important serotypes.	listeria monocytogenes is a gram-positive, facultative intracellular bacterium that causes invasive, often fatal, disease in susceptible hosts. as a foodborne pathogen, the bacterium has emerged as a significant public health problem and has caused several epidemics in the united states and europe. three serotypes (1/2a, 1/2b, 4b) of l. monocytogenes are responsible for nearly 95% of all reported cases of human listeriosis. l. monocytogenes serotype 4b has caused all well-characterized foodborne ...	1998	9541569
[listeria monocytogenes-induced endogenous endophthalmitis in an otherwise healthy patient: pcr-assisted rapid diagnosis as the basis for successful therapy].	listeria monocytogenes is a rare cause of endogenous endophthalmitis. only 14 cases are published in the literature so far. all eyes showed similar clinical features and profound visual loss.	1998	9541896
comparison of pcr-based dna fingerprinting techniques for the identification of listeria species and their use for atypical listeria isolates.	four pcr-based dna fingerprinting techniques were compared for their ability to identify at the species level a heterogeneous collection of isolates belonging to the six valid listeria species. 16s rdna-rflp analysis identified all species and 16s rdna-sscp analysis identified almost all species. also, isolates with unusual biochemical characteristics and/or unusual antigenic composition could be identified correctly. rrna-intracistronic length polymorphism analysis suffered from high intraspeci ...	1998	9542083
attachment of group b streptococci to macrophages is mediated by a 21-kda protein.	group b streptococcus (gbs) is able to bind to human macrophages in vitro in the absence of exogenous opsonins. the exact mechanisms that mediate this attachment are unclear. this study was undertaken to determine what protein adhesins are present on the surface of gbs that mediate attachment to macrophages. we have identified a 21-kda protein from the envelope of gbs type iii that directly binds to macrophages as determined by western blot analysis. antiserum against this protein was able to in ...	1998	9544775
neuropeptides in the livers of mice during bacterial infections.	neuropeptides such as substance p (sp) and vasoactive intestinal peptide (vip) are known to act as immunomodulators. we investigated the induction of sp and vip in the livers of mice infected with listeria monocytogenes or injected with tsukamurella paurometabolum. vip was detected in the livers of mice after l. monocytogenes infection by an immunohistochemical technique and preprovip mrna, which was detected by reverse transcription-polymerase chain reaction (pcr), was induced post infection. h ...	1998	9544784
the effects of inoculation of listeria monocytogenes into the ovine mammary gland.	in each of two experiments, the effects of inoculation of listeria monocytogenes into the ovine mammary gland were studied. in the first experiment, ewes were challenged with one or other of five different listeria spp. isolates to study differences in their pathogenicity. in the second, ewes were challenged with l. monocytogenes serotype 1/2a to study the sequential features of the infection. the reaction of the mammary glands was assessed by bacteriological, cytological and histological method ...	1998	9549859
mycobacterium bovis bacille calmette-guérin strains secreting listeriolysin of listeria monocytogenes.	recombinant (r) mycobacterium bovis strains were constructed that secrete biologically active listeriolysin (hly) fusion protein of listeria monocytogenes. the r-bcg strains pat261:hly or pmv306:hly expressed plasmid multicopies or chromosomal single copies of the hly gene, respectively. human and murine macrophage-like cell lines were infected with r-bcg pat261:hly and pmv306:hly strains. interestingly, intracellular persistence of both r-bcg strains was reduced in macrophages as compared with ...	1998	9560270
prevalence of listeria species in raw milk from farm bulk tanks in nagano prefecture.	raw milk samples from bulk tanks of a total of 943 farms, which corresponded to approximately 60% of all dairy farms in nagano prefecture were examined for listeria species between december 1990 and april 1991. listeria spp. were isolated from 29 (3.1%) of 943 milk specimens. in the southern, central, eastern and northern areas of the prefecture, listeria spp. were isolated from 6.1% (22/362), 1.5% (4/272), 1.4% (2/143) and 0.6% (1/166) of samples, respectively. listeria monocytogenes was isolat ...	1998	9560778
activation and suppression of natural cellular immune functions by pneumocystis carinii.	the regulatory role of soluble cytokines in innate cellular immune responses induced by pneumocystis carinii was assessed in vitro in direct comparison to induction by listeria monocytogenes. this report shows that p. carinii organisms, as well as l. monocytogenes, stimulated in whole spleen cell cultures of scid mice the release of ifn-gamma, tnf-alpha/beta, il-10, il-12, and ino. this response was independent of functional t cells. both macrophages (m phi) and natural killer (nk) cells were ne ...	1998	9562861
antimicrobial properties of plant essential oils and essences against five important food-borne pathogens.	the antimicrobial properties of 21 plant essential oils and two essences were investigated against five important food-borne pathogens, campylobacter jejuni, salmonella enteritidis, escherichia coli, staphylococcus aureus and listeria monocytogenes. the oils of bay, cinnamon, clove and thyme were the most inhibitory, each having a bacteriostatic concentration of 0.075% or less against all five pathogens. in general, gram-positive bacteria were more sensitive to inhibition by plant essential oils ...	1998	9569693
inhibition effect of enterocin ccm 4231 in the rumen fluid environment.	enterocin ccm 4231 is a bacteriocin with a broad antimicrobial spectrum produced by the ruminal strain enterococcus faecium ccm 4231. its inhibitory effect towards enterococci, ent. faecium ef 26/42, staphylococci, streptococcus bovis ao 24/85 and escherichia coli, as well as towards listeria monocytogenes ohio strain, in the rumen fluid environment was studied during culture at 37 and 30 degrees c for 24 h and 20 days. enterocin ccm 4231 was added to the samples at a concentration of 3200 au ml ...	1998	9569712
plantaricin d, a bacteriocin produced by lactobacillus plantarum bfe 905 ready-to-eat salad.	lactobacillus plantarum bfe 905 isolated from 'waldorf' salad produced a bacteriocin termed plantaricin d which was active against lact. sake and listeria monocytogenes strains. plantaricin d was heat stable, retaining activity after heating at 121 degrees c. the bacteriocin was inactivated by alpha-chymotrypsin, trypsin, pepsin and proteinase k, but not by papain and other non-proteolytic enzymes tested. plantaricin d was stable at ph values ranging from 2.0 to 10.0. the bacteriocin inhibited g ...	1998	9569716
protective effect of administration of skim milk on exogenous and endogenous infection in mice.	in order to minimize the denaturation of proteins in milk, normal cow's milk was pasteurized at 61 c for 20 min. the protective effects of the thus prepared skim milk (low-heat skim milk) on exogenous and endogenous infection were examined as compared with conventional skim milk which was pasteurized at 121 c for 2 sec. the antibody titers to listeria monocytogenes and escherichia coli of low-heat skim milk were almost equal to that of raw milk, while no antibody was detected in the conventional ...	1998	9570286
the clpc atpase of listeria monocytogenes is a general stress protein required for virulence and promoting early bacterial escape from the phagosome of macrophages.	under stress conditions, the facultative intracellular pathogen listeria monocytogenes produces a clpc atpase, which is a general stress protein encoded by clpc and belonging to the hsp-100/clp family. a clpc-deficient mutant was obtained by gene disruption in strain lo28, which became highly susceptible to stress conditions in vitro. intracellular growth of this mutant was restricted within macrophages, one of the major target cells of l. monocytogenes, during the infectious process. a quantita ...	1998	9570408
evaluation of five imidazopyrazinone-type chemiluminescent superoxide probes and their application to the measurement of superoxide anion generated by listeria monocytogenes.	superoxide-triggered chemiluminescence of five new imidazopyrazinone derivatives was investigated using the hypoxanthine-xanthine oxidase system as the source of superoxide anion. the results showed that they are highly sensitive and have favorable properties in measuring superoxide anion. with those new probes, the generation of superoxide anion from the bacteria listeria monocytogenes was examined. the results confirmed the previous report that l. monocytogenes is an unusual organism that extr ...	1998	9570834
sequence analysis of the acta gene of listeria monocytogenes isolated from human.	the region encoding proline-rich units of acta genes was amplified from 24 strains of listeria monocytogenes using polymerase chain reaction (pcr). pcr products of 13 strains showed the expected size of 623 bp, whereas those of 11 strains showed a short size of 518 bp. the shortening of these pcr products resulted from the deletion of one proline-rich unit. these results indicate that acta proteins are divided into at least two different types which are unrelated to bacterial serotypes.	1998	9572045
production of proinflammatory cytokines and inflammatory mediators in human intestinal epithelial cells after invasion by trichinella spiralis.	epithelial cells are the first point of host contact for invasive intestinal pathogens and may initiate mucosal inflammatory responses via production of proinflammatory cytokines and mediators. the aim of the present study was to investigate in vitro the initial invasion of a parasitic nematode (trichinella spiralis), to measure the early production of specific epithelial cytokines and inflammatory mediators after invasion, and to compare these responses with those to invasive bacteria. monolaye ...	1998	9573108
disruption of the cellular inflammatory response to listeria monocytogenes infection in mice with disruptions in targeted genes.	the results of this study to dissect the nature of the acquired immune response to infection with listeria monocytogenes in mice with targetted gene disruptions show that successful resolution of disease requires the essential presence of alphabeta t cells and the capacity to elaborate gamma interferon. in the absence of either of these entities, mice experience increasingly severe hepatitis and tissue necrosis and die within a few days. the data from this study support the hypothesis that the p ...	1998	9573119
roles for tumor necrosis factor and gamma interferon in resistance to enteric listeriosis.	listeria monocytogenes normally infects the host by translocating from the intestinal lumen. experiments were carried out to determine if, when, and where tumor necrosis factor (tnf) and gamma interferon (ifn-gamma) function in antibacterial resistance during enteric listeriosis. groups of normal mice and severe combined immunodeficient (scid) mice were injected with neutralizing monoclonal antibodies (mab) specific for each cytokine and then inoculated intragastrically with l. monocytogenes. th ...	1998	9573134
effects of endotoxemia and sepsis on bilirubin oxidation by rat brain mitochondrial membranes.	sepsis is believed to increase the risk of bilirubin brain toxicity, but the mechanism is not known. adult male sprague-dawley rats were injected intraperitoneally with either 20 mg/kg escherichia coli lipopolysaccharide, approximately 5 x 10(9)/kg cfu listeria monocytogenes or vehicle 48 h prior to sacrifice. rats were killed with an intraperitoneal injection of pentobarbital. mitochondrial membrane fractions were produced by homogenization of the brains and differential centrifugation in 0.32 ...	1998	9573465
mhc class i/peptide stability: implications for immunodominance, in vitro proliferation, and diversity of responding ctl.	infection of balb/c mice with listeria monocytogenes primes cd8+ cytotoxic t cells specific for four different h2-kd-restricted peptides. in vitro restimulation of l. monocytogenes immune splenocytes with each of these peptides resulted in larger t cell responses to p60 217-225 and mpl 84-92 than to llo 91-99 and p60 449-457. direct frequency analyses of immune splenocytes, however, revealed that llo 91-99 and p60 217-225 elicit dominant t cell responses, while p60 449-457 and mpl 84-92 elicit m ...	1998	9574549
computerized analysis of restriction fragment length polymorphism patterns: comparative evaluation of two commercial software packages.	two computerized restriction fragment length polymorphism pattern analysis systems, the bioimage system and the gelcompar system (molecular analyst fingerprinting plus in the united states), were compared. the two systems use different approaches to compare patterns from different gels. in gelcompar, a standard reference pattern in one gel is used to normalize subsequent gels containing lanes with the same reference pattern. in bioimage, the molecular sizes of the fragments are calculated from s ...	1998	9574697
ampicillin-aminoglycoside interaction studies using listeria monocytogenes.		1998	9578172
pyrimethamine impairs host resistance to infection with listeria monocytogenes in balb/c mice.	increased mortality has been observed when hiv-infected patients were treated with pyrimethamine (pyr) as prophylaxis for toxoplasmic encephalitis, suggesting that pyr might possess immunosuppressive activity. to analyze this in an animal model, immune function was assessed in balb/c mice using a battery of in vivo and ex vivo assays and an in vivo model of host resistance to listeria monocytogenes infection. treatment for 30 days with 60 mg/kg pyr decreased circulating white blood cell and lymp ...	1998	9579021
listeria monocytogenes rhomboencephalitis with cranial-nerve palsies: a case report.	listeria monocytogenes rhomboencephalitis is an uncommon complication of l. monocytogenes meningitis. it presents in a typical biphasic pattern characterized by a non-specific prodromal period followed by any combination of asymmetrical, cranial-nerve palsies; cerebellar signs; hemiparesis or hypesthesia; and diminished consciousness. the survival rate is greater than 70% when appropriate antibiotic therapy is initiated early. however, approximately 60 percent of the survivors develop neurologic ...	1998	9581507
neural wiskott-aldrich syndrome protein is implicated in the actin-based motility of shigella flexneri.	shigella, the causative agent of bacillary dysentery, is capable of directing its own movement in the cytoplasm of infected epithelial cells. the bacterial surface protein virg recruits host components mediating actin polymerization, which is thought to serve as the propulsive force. here, we show that neural wiskott-aldrich syndrome protein (n-wasp), which is a critical target for filopodium formation downstream of cdc42, is required for assembly of the actin tail generated by intracellular s.f ...	1998	9582270
h2-m3 restricted presentation of a listeria-derived leader peptide.	protective immunity to infection by many intracellular pathogens requires recognition by cytotoxic t lymphocytes (ctls) of antigens presented on major histocompatibility complex (mhc) class i molecules. to be presented for recognition by pathogen-specific ctls, these antigens must gain access to the host cell class i processing pathway. in the case of intracellular bacterial pathogens, the majority of bacterial proteins are retained within the bacterial membrane and therefore remain inaccessible ...	1998	9584149
internalin b is essential for adhesion and mediates the invasion of listeria monocytogenes into human endothelial cells.	listeria monocytogenes causes rhombencephalitis in humans and animals and also affects the fetus in utero, causing disseminated sepsis. in both instances, the infection occurs by the crossing of endothelial cells lining a physiological barrier, the blood-brain barrier or the transplacental barrier. in this study, the ability of l. monocytogenes wild-type egd to invade human umbilical vein endothelial cells (huvecs) was evaluated using wild-type bacteria and isogenic listeria mutants. here, we sh ...	1998	9593298
host cell protein tyrosine kinases are activated during the entry of listeria monocytogenes. possible role of pp60c-src family protein kinases.	listeria monocytogenes is able to invade a wide range of cell types by inducing its own internalization. little is known, however, about the host cell proteins affecting the entry process which involves triggering the host cell signal transduction mechanism. we report here that entry of l. monocytogenes strains (serotypes 4b and 1/2a) into caco-2 cells induced tyrosine phosphorylation of several host cell proteins including pp60c-src substrates. using specific synthetic peptide substrates, we sh ...	1998	9595679
cytochalasin-induced actin disruption of polarized enterocytes can augment internalization of bacteria.	cytochalasin-induced actin disruption has often been associated with decreased bacterial internalization by cultured epithelial cells, although polarized enterocytes have not been systematically studied. in assays using confluent polarized ht-29 enterocytes, cytochalasin d appeared to increase internalization of wild-type salmonella typhimurium, proteus mirabilis, and escherichia coli. hela and hep-2 epithelial cells, as well as ht-29 and caco-2 enterocytes, were used to clarify this unexpected ...	1998	9596696
secretory phospholipase a2 is the principal bactericide for staphylococci and other gram-positive bacteria in human tears.	we examined human tears for molecules that killed gram-positive bacteria. the principal mediator of bactericidal activity against staphylococci proved to be a calcium-dependent enzyme, secretory phospholipase a2. whereas the concentration of secretory phospholipase a2 in the normal tear film exceeded 30 microg/ml, only 1.1 ng (<0.1 nm) of the enzyme per ml sufficed to kill listeria monocytogenes and 15 to 80 ng/ml killed staphylococcus aureus. despite its efficacy against gram-positive bacteria, ...	1998	9596749
listeria monocytogenes-infected hepatocytes are targets of major histocompatibility complex class ib-restricted antilisterial cytotoxic t lymphocytes.	subclinical infection of balb/c mice with the intracellular bacterial pathogen listeria monocytogenes results in the development of protective antilisterial immunity. l. monocytogenes can infect hepatocytes, and antilisterial cytotoxic t lymphocytes (ctl) lyse listeria-infected hepatocytes in a major histocompatibility complex (mhc) class ia-restricted manner. it remained to be determined whether l. monocytogenes-infected hepatocytes are susceptible to mhc class ib-restricted cytolysis. in this ...	1998	9596753
endogenous interleukin-4 does not suppress the resistance against a primary or a secondary listeria monocytogenes infection in mice.	interleukin-4 (il-4), a cytokine produced by t-helper 2 (th2) cells, can inhibit the development of t-helper 1 (th1) cells, which results in a decreased release of cytokines by the latter. as interferon-gamma (ifn-gamma), produced by th1 cells, is involved in the resistance against a listeria monocytogenes infection, the role of endogenously formed il-4 during a listeria infection in mice was investigated. neutralization of endogenously formed cytokines by subcutaneously injected alginate-encaps ...	1998	9600319
synergistic effect of nisin and the lactoperoxidase system on listeria monocytogenes in skim milk.	nisin added at 10 or 100 iu/ml to ultra-high temperature processed (uht) skim milk had no effect on counts of listeria monocytogenes after 24 h at 30 degrees c, whereas addition of the lactoperoxidase system (lps) resulted in counts of viable cells three log units lower than those of control milk after 24 h at 30 degrees c. addition of nisin and lps showed a synergistic effect and resulted in counts up to 5.6 log units lower than the control milk. when the two preservatives were added to activel ...	1998	9600608
the combined effect of nisin, leucocin f10, ph, nacl and edta on the survival of listeria monocytogenes in broth.	the combined effect of the bacteriocins nisin (1-2100 iu/ml) and leucocin f10 (1-2100 au/ml), ph (4.7-6.5), nacl (0.7-4.5% w/l), ethylene diaminetetraacetic acid disodium salt (edta, 0.08-4.72 mmol/l) and inoculum level (10(3)-10(8) cfu/ml) on the survival of a pool of three strains of listeria monocytogenes in broth was evaluated in three factorial experiments. several factor combinations were found to prevent growth. logistic regression analysis of the categorical data (survival/no survival) w ...	1998	9600612
enhanced detection and enumeration of listeria monocytogenes from foodstuffs and food-processing environments.	listeria monocytogenes blood agar (lmba) was compared to listeria selective agar based on lithium chloride and ceftazidime (la) and to the oxford and palcam media recommended by iso and idf for the detection and enumeration of l. monocytogenes from foodstuffs and food-processing environments. lmba is based on trypticase soy agar with the following additions: sheep blood (5%) and as selective agents lithium chloride (10 g/l), polymyxin b sulphate (10 mg/l) and ceftazidime (20 mg/l), whereas the s ...	1998	9600613
predicted and observed growth of listeria monocytogenes in seafood challenge tests and in naturally contaminated cold-smoked salmon.	the performance of the pathogen modelling program, the food micromodel, the murphy-model and the ross-model for growth of l. monocytogenes was evaluated by comparison with data from 100 seafood challenge tests and data from 13 storage trials with naturally contaminated sliced vacuum-packed cold-smoked salmon. challenge tests with both cured and noncured products were studied, and graphs as well as the bias- and the accuracy factors were used for comparison of the observed and predicted growth. t ...	1998	9600616
production of bacteriocin-like-substance by listeria innocua against listeria monocytogenes.	cultures and culture filtrates of 129 listeria innocua strains were examined for inhibitory activity against 18 strains of listeria monocytogenes. of the strains examined, 114 (88.4%) cultures and 126 (97.7%) culture filtrates had an inhibitory activity against strains of l. monocytogenes and most filtrates were sensitive to trypsin treatment. the authors concluded that most l. innocua strains produce a trypsin sensitive bacteriocin-like substance against l. monocytogenes.	1998	9600620
effects of above-optimum growth temperature and cell morphology on thermotolerance of listeria monocytogenes cells suspended in bovine milk.	the thermotolerances of two different cell forms of listeria monocytogenes (serotype 4b) grown at 37 and 42.8 degrees c in commercially pasteurized and laboratory-tyndallized whole milk (wm) were investigated. test strains, after growth at 37 or 42.8 degreesc, were suspended in wm at concentrations of approximately 1.5 x 10(8) to 3.0 x 10(8) cells/ml and were then heated at 56, 60, and 63 degrees c for various exposure times. survival was determined by enumeration on tryptone-soya-yeast extract ...	1998	9603815
listeria monocytogenes in crohn's disease.	in an immunohistochemical study a higher rate of reactivity of intestinal tissues to the antibody against listeria monocytogenes was reported in crohn's disease as compared with controls.	1998	9605266