Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| immunoglobulin m-specific enzyme-linked immunosorbent assay for serodiagnosis of bovine respiratory syncytial virus infections. | an antibody-capture enzyme-linked immunosorbent assay (elisa) for detection of immunoglobulin (ig) m antibodies to bovine respiratory syncytial virus (brsv) in cattle was developed. monoclonal antibody to bovine igm was used as the catching antibody. the igm-elisa was used, as well as a brsv-specific igg elisa to determine the kinetics of igm and igg antibody responses to brsv infections in cattle. high igm and igg antibody titers developed after naturally occurring or induced brsv infection of ... | 1987 | 3631699 |
| respiratory syncytial virus infections in adults. | in a retrospective 10-year study of 57 adult patients admitted to orebro medical center hospital, sweden, with rs virus infections, pneumonia was diagnosed in 36. a primary episode of obstructive airway symptoms was observed in 20% of the patients with pneumonia. several patients had a long period of fever and a protracted stay in the hospital. no fatal cases or serious complications were registered. compromised hosts did not appear to be overrepresented in the material, nor did patients with im ... | 1987 | 3631728 |
| efficacy of ribavirin aerosol treatment for respiratory syncytial virus bronchiolitis in infants. | the therapeutic efficacy of ribavirin, an anti-viral agent with a broad spectrum of activity, was studied in 14 infants with bronchiolitis which, in seven cases, was caused by a respiratory syncytial virus infection. the drug was administered for 5-6 days using an aerosol in periods of either 4 or 6 h with an interval of either 4 or 2 h between administrations. the body temperature of patients with fever returned to normal by day 3 of therapy. a significant decrease in the respiratory rate occur ... | 1987 | 3653500 |
| rapid virus subunit visualization by direct sedimentation of samples on electron microscope grids. | airfuge direct ultracentrifugation of viral samples on electron microscope grids offers a rapid way for concentrating viral particles or subunits to facilitate their detection and study. using the a-100 fixed angle rotor (30 degrees) with a k factor of 19 at maximum speed (95,000 rpm), samples up to 240 microliters can be prepared for electron microscopy observation in a few minutes: observation time is decreased and structural details are highlighted. using latex spheres to calculate the increa ... | 1987 | 3654898 |
| freeze-drying of respiratory syncytial viruses for transportation and storage. | the stabilities of eight strains of respiratory syncytial virus were compared after the strains were freeze-dried in the presence and absence of the stabilizer spga, which contains 218 mm sucrose, 7.1 mm dipotassium hydrogen phosphate, 3.76 mm potassium dihydrogen phosphate, 4.9 mm sodium glutamate, and 1% (wt/vol) bovine albumin. strains freeze-dried in the presence of spga showed only small-to-negligible losses at 4 degrees c and losses of approximately 2.0 log10 infectious units at 25 degrees ... | 1987 | 3654949 |
| demonstration that glycoprotein g is the attachment protein of respiratory syncytial virus. | two monospecific rabbit antisera to the g glycoprotein, one induced with purified g and the second with a recombinant vaccinia virus containing the gene for g, inhibit the attachment of purified [35s]methionine-labelled long strain of respiratory syncytial virus to monolayers of hela cells. attachment was not inhibited by monospecific rabbit antisera to glycoprotein f induced with either purified f or with a recombinant vaccinia virus containing the gene for f. | 1987 | 3655746 |
| respiratory syncytial virus infection and cardiopulmonary disease. | 1987 | 3658500 | |
| environmental exposure of primary care personnel to ribavirin aerosol when supervising treatment of infants with respiratory syncytial virus infections. | the potential exposure to ribavirin aerosol in the environment was assessed in nurses caring for infants and children with severe lower respiratory tract infections due to respiratory syncytial virus. ribavirin aerosol was administered via a ventilator, oxygen tent, or oxygen hood. participants worked directly with infants receiving ribavirin for 20.0 to 35.0 h over a 3-day period. no toxic or adverse effects of ribavirin aerosol were observed in any of the 19 nurses studied, and ribavirin was n ... | 1987 | 3662474 |
| vaccines for respiratory disease in cattle. | respiratory disease is one of the most serious disease complexes affecting beef cattle production. for example, it is claimed to cost the uk industry about 70 million pounds per year. it is usually associated with young cattle and can occur in a variety of situations. it is a good example of multifactorial disease in that its aetiology involves both infection by a variety of microorganisms and a number of environmental factors. several distinct syndromes occur and a number of microorganisms are ... | 1987 | 3673201 |
| transient neutropenia of childhood. | patient characteristics and clinical course are described in 21 children with newly discovered neutropenia (absolute neutrophil count less than 1500/microliter). only children over age 3 months are included; 19 of 21 were less than age 2 years. the majority had respiratory tract infections and 11 had been on various medications at the time neutropenia was discovered. bacteria were isolated from the blood of three patients (s. pneumoniae in two, h. influenzae in one) and from urine in one (e. col ... | 1987 | 3677533 |
| a technique for the administration of ribavirin to mechanically ventilated infants with severe respiratory syncytial virus infection. | fifteen infants with respiratory syncytial virus pulmonary infection admitted to our pediatric icu from december 1, 1985 through april 30, 1986, required mechanical ventilation. these patients were placed on an open trial of ribavirin therapy. we describe a technique for the safe delivery of aerosolized ribavirin to these infants while on the ventilator. the agent was delivered for 16 h/day for 7 days. modifications of the ventilator circuit were needed to prevent the condensation of the drug in ... | 1987 | 3677748 |
| strain variation of respiratory syncytial virus. | western blotting and immunoperoxidase staining with monoclonal antibodies were employed to analyse epitopic and polypeptide molecular weight variation among isolates of respiratory syncytial (rs) virus collected in newcastle between 1965 and 1986. one group of isolates resembled the a2 and long prototype subgroup a strains of rs virus in possessing a p protein of mr 34,000. isolates in this subgroup showed two patterns of reactivity with subgroup a-specific monoclonal antibodies to the g glycopr ... | 1987 | 3681263 |
| molecular cloning and sequencing of the f and 22k membrane protein genes of the rss-2 strain of respiratory syncytial virus. | the nucleotide sequences of the adjacent genes coding for the f protein and 22k protein of the rss-2 strain of human respiratory syncytial (rs) virus were determined from cdna clones of genomic rna. comparison of these sequences and the inferred amino acid sequences of the f and 22k proteins with the corresponding published sequences of another subgroup a virus, the a2 strain of rs virus, reveals extensive overall homology (greater than 95%) at both the nucleotide and amino acid levels, even tho ... | 1987 | 3681264 |
| isotype-specific elisas for the detection of antibodies to bovine respiratory syncytial virus. | isotype-specific elisas for the detection of antibodies to bovine respiratory syncytial virus (brsv) are described. brsv-specific igg1 and igg2 were determined in indirect double antibody sandwich assays. for iga and igm antibody capture assays were used. the isotype specificity of the assays was confirmed by the observation that samples with a high titre of brsv-specific antibodies of particular isotype were negative in the assays for the other isotypes and vice versa. comparison of the results ... | 1987 | 3685631 |
| gene overlap and site-specific attenuation of transcription of the viral polymerase l gene of human respiratory syncytial virus. | sequence analysis of the gene encoding polymerase l protein of respiratory syncytial virus showed that l-gene transcription initiates within its upstream neighbor, the gene encoding the 22-kda protein 22k. this is an exception to the canonical mode of sequential transcription of nonoverlapping genes described for other nonsegmented negative-strand rna viruses. as a consequence of the gene overlap, the termination/polyadenylylation signal for the 22k gene is located at nucleotides 56-68 within th ... | 1987 | 2440043 |
| modulation of monocyte mo2 surface antigen expression by exposure to respiratory syncytial virus. | the mo2 antibody recognizes a monocyte-specific cell surface antigen of unknown function. upward modulation of mo2 surface epitope density was demonstrated in response to 72-hr culture of monocytes with respiratory syncytial virus (rsv), but this was not seen after culture with phytohemagglutinin or other respiratory viruses. monoclonal antibody probes for rsv proteins were used to demonstrate probable replication of rsv proteins in peripheral blood monocytes and pulmonary alveolar macrophages. ... | 1987 | 2440300 |
| monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. | employing different routes of immunization and different adjuvants, a large number of hybridomas were produced that secreted monoclonal antibodies (mabs) against the large envelope glycoprotein (gp90) and the fusion glycoprotein (gp70) of respiratory syncytial virus (rsv). the antigenic specificity of the mabs was established by immunoblot analysis and radioimmunoprecipitation. three hybridomas secreting anti-gp90 mabs were established from mice immunized by intraperitoneal injection of purified ... | 1987 | 2440982 |
| the g glycoprotein of human respiratory syncytial viruses of subgroups a and b: extensive sequence divergence between antigenically related proteins. | two major antigenic subgroups (designated a and b) have been described for human respiratory syncytial virus (rsv). previously, on the basis of reactivity patterns with monoclonal antibodies, the greatest intersubgroup variation was shown to occur in the g protein, the putative attachment glycoprotein. to delineate the molecular basis for this variation, we have determined the nucleotide and deduced amino acid sequences of the g mrnas and proteins representing a subgroup a (long strain) and a su ... | 1987 | 2441388 |
| identification of a synthetic peptide as part of a major neutralization epitope of respiratory syncytial virus. | a 7000 mr cleavage fragment of the f1 subunit that carries the major neutralization epitope has been identified by chemical and enzymatic cleavage of the fusion protein of respiratory syncytial (rs) virus (long strain) with an efficient rs virus-neutralizing monoclonal antibody. based on the published mrna-deduced sequence of the a2 strain, coupled to the hydropathicity profile and prediction of protein conformation, the neutralization epitope has tentatively been localized on the first third of ... | 1987 | 2443612 |
| respiratory syncytial virus fusion glycoprotein: further characterization of a major epitope involved in virus neutralization. | competition experiments and biological assays with a panel of 15 monoclonal antibodies confirmed the presence of at least four antigenic sites on the fusion protein of human respiratory syncytial virus, three of which were involved in virus neutralization. one antigenic site, recognized by two strongly neutralizing antibodies, was conserved after reduction and denaturation and shown by immunoblotting to be localized on the f1 fragment of the fusion protein. cleavage of this protein with staphylo ... | 1987 | 2446731 |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup b. | mouse hybridomas producing antibodies against the structural proteins of strain wv4843, a subgroup b strain of respiratory syncytial (rs) virus, were produced by fusion of sp2/0 myeloma cells with spleen cells from balb/c mice immunized with purified preparations of the virus. after immunoprecipitation test with [35s]methionine-labelled extracellular virions, 35 clones found to produce antibodies against the fusion (f) protein, six against the member (m) protein, 21 against the nucleocapsid (np) ... | 1987 | 2447224 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on virus interference. | during the 7-year period from september 1978 to august 1985, smear specimens of nasopharyngeal secretions from 3132 patients mainly hospitalized children, taken in different regions in norway, were examined for respiratory viruses by the rapid immunofluorescence (if) technique. a positive diagnosis for respiratory syncytial virus (rsv), parainfluenza virus type 1, 2 and 3 or influenza a and b virus was made for 896 patients (29%). the greatest prevalence for all these viruses was observed during ... | 1987 | 2824225 |
| cryogenic preservation of virus-infected cells used as immunofluorescent assay substrates. | cells infected with herpes simplex virus, respiratory syncytial virus and adenovirus were subjected to several cryogenic regimens to determine whether these procedures were detrimental to the performance and results obtained with an indirect immunofluorescence assay (ifa). infected cells were harvested and divided into equal aliquots prior to freezing at different temperatures (-20, -70 and -165 degrees c). cells fixed onto glass slides immediately after harvesting were used as controls. for the ... | 1987 | 2824546 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| epidemiology of viral infections in singapore. | as singapore is a densely populated island, and also a major air and sea port, the importation and dissemination of viral infections is facilitated. respiratory viral infections have the highest prevalence rates, influenza and respiratory syncytial virus (rsv) being the most important ones. seasonal variation occurs with influenza, rsv and parainfluenza virus type 1 infections. the age distribution and clinical picture associated with infections due to the various respiratory viruses are similar ... | 1987 | 2825585 |
| hospital-acquired pneumonia in children: the role of respiratory viruses. | the most frequent agents of pneumonia acquired by children in the hospital are viruses. the characteristics of these nosocomial viral agents differ appreciably from the more classical bacterial nosocomial infections. the viral agents tend to be more contagious, with normal children being as susceptible as the predisposed, high-risk child. the epidemiology of these hospital-acquired infections tends to mimic the patterns of activity of the respiratory viruses in the community. the major causes of ... | 1987 | 2827278 |
| pulmonary infections with respiratory syncytial virus and the parainfluenza viruses. | respiratory syncytial virus (rsv) and the parainfluenza viruses (pivs) are the most important causes of acute lower respiratory illness (lri) in infants and children under 6 years of age. these enveloped viruses are members of the paramyxovirus family. they infect cells in the epithelium lining the trachea and intrapulmonary airways, and cause croup, bronchitis, bronchiolitis, and bronchopneumonia. rsv causes annual midwinter to early spring outbreaks of respiratory disease in temperate climates ... | 1987 | 2827280 |
| [infectious respiratory tract diseases in cattle in the winter of 1985/86: detection of infection by bovine respiratory syncytial virus and parainfluenza virus type 3 by immunofluorescence in nasal swab samples and by antibody titer increase in serum (elisa)]. | 1987 | 2827303 | |
| protection against respiratory disease in calves induced by vaccines containing respiratory syncytial virus, parainfluenza type 3 virus, mycoplasma bovis and m dispar. | a field trial to assess the ability of two vaccines to protect calves against respiratory disease was carried out on a large beef rearing unit in southern england over the two winters of 1983 to 1984 and 1984 to 1985. a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma bovis and m dispar or a vaccine containing only the respiratory syncytial virus component were inoculated into 246 and 245 calves, respectively; 245 calves r ... | 1987 | 2827366 |
| comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. | paired nasopharyngeal aspirate (npa) and nasopharyngeal swab (nps) specimens obtained from each of 32 hospitalized infants with x-ray-confirmed pneumonia (91%) or bronchiolitis were tested for respiratory syncytial virus (rsv) infection by virus culture, the indirect immunofluorescent-antibody (ifa) technique, enzyme-linked immunosorbent assay (elisa; ortho diagnostic systems, inc.), and spot hybridization with a human genomic probe to quantitate cellular dna. rsv was isolated in cell cultures f ... | 1987 | 3294883 |
| diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. | with the availability of ribavirin therapy for serious respiratory syncytial virus (rsv) infections, rapid diagnostic tests for the detection of rsv antigen are increasingly important. efficacies of a commercially available enzyme immunoassay (eia) (abbott laboratories, north chicago, ill.) and a fluorescent-antibody assay (fa) were evaluated in a study involving 135 specimens from children with respiratory symptoms. a nasal wash specimen was cultured immediately on rsv-sensitive a549 cells; the ... | 1987 | 3294889 |
| efficiency of immunofluorescence for rapid detection of common respiratory viruses. | rapid immunofluorescence (fa) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of fa in a clinical laboratory setting. for respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). the sensitivity of fa for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza a virus, and adenoviruses ranged from 28 to 63%, but specificities for th ... | 1987 | 3029168 |
| diseases of wapiti utilizing cattle range in southwestern alberta. | specimens from 28 wapiti (cervus elaphus canadensis) were collected by hunters in southwestern alberta in 1984. various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). no sero ... | 1987 | 3029443 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the matrix protein. | the sequence of the matrix (m) protein gene and contiguous intergenic regions of the human parainfluenza 3 virus (pf3) was determined by molecular cloning. the encoded m protein contains 354 amino acids and has a predicted mol wt of 39,506. the m protein amino acid sequence was compared to the homologous proteins from other members of the paramyxoviridae family. the pf3 protein shared 61% homology with the sendai virus protein and approximately 35% homology with measles and canine distemper viru ... | 1987 | 3029963 |
| seroepidemiological studies on the occurrence of common respiratory infections in paediatric student nurses and medical technology students. | the occupational risk of acquiring minor respiratory infections for paediatric student nurses was estimated by performing serological examinations with influenza a, b, c, parainfluenza, mumps, respiratory syncytial virus, adenovirus and mycoplasma pneumoniae at 6-month intervals over a period of 4 years in paediatric student nurses at two schools of nursing and students at one school of medical technology. titre increases against all tested agents occurred 1.86 times more often in the student nu ... | 1987 | 3030790 |
| preparation of nasopharyngeal secretions for immunofluorescence by one-step centrifugation through percoll. | a simple method was developed for separation of cells from nasopharyngeal secretion for the diagnosis of respiratory virus infections by immunofluorescence microscopy. the diluted specimen, containing dithiothreitol to break up the mucus, was centrifuged once through a cushion of 20% percoll (colloidal silica, a density gradient medium), which permitted sedimentation of cells through the cushion, but retained mucus on top of it. the pelleted cells were resuspended, and microscope slides were the ... | 1987 | 3034944 |
| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| epstein-barr virus, cytomegalovirus, and other viral infections in children after liver transplantation. | we studied 51 consecutive pediatric patients for the frequency and morbidity of viral infections after liver transplantation. the incidence of primary (67%) and reactivation (48%) epstein-barr virus (ebv) infections and reactivation (88%) cytomegalovirus (cmv) infection was comparable to that seen in adult transplant recipients. however, fewer pediatric than adult transplant recipients experienced primary cmv infection (p less than .01). five (38%) of 13 cmv infections were symptomatic and inclu ... | 1987 | 3036964 |
| pneumonias in adults due to mycoplasma, chlamydiae, and viruses. | pneumonias in adults due to mycoplasma, chlamydiae, and viruses are a common clinical problem. these microorganisms contribute to the etiologies in 6-35% of all cases of pneumonia and are the sole pathogens in 1-17% of hospitalized cases. important trends and developments in the field include the emergence of a chlamydia psittaci strain (twar) that is passaged from human to human, causes a mycoplasma-like illness, and that is relatively resistant to erythromycin, the recognition of respiratory s ... | 1987 | 3037901 |
| cell-mediated cytotoxic response to respiratory syncytial virus in infants with bronchiolitis. | a cell-mediated cytotoxic response to respiratory syncytial (rsv) was demonstrated in the peripheral blood of 4 of 22 infants with acute bronchiolitis. these 4 infants were aged 3 weeks to 3 months. no such response was found in infants older than 4 months. all 4 infants with a positive response had mild infections. | 1987 | 2888988 |
| rapid detection of respiratory syncytial virus in clinical specimens. | results of the direct immunofluorescence (if) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (rsv) isolation. the prevalence of rsv infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. the average rsv isolation rate was 18.42%, whereas the virus antigen detection was positive in 19.74% of cases. the agreement between virus isolation and direct if was 92.1%, the sensitivity of if being 82. ... | 1987 | 2891280 |
| [respiratory syncytial virus infections and apnea in low birth-weight infants]. | respiratory syncytial virus infection was observed in 20 infants of less than 6 months of age who had a low weight at birth, were premature and/or presented with intra-uterine growth retardation. except for rhinitis, which was present in all cases, the symptoms were closely correlated to the gestational age: beyond 48 weeks they were those of a common lower respiratory tract infection, but below 48 weeks attacks of apnea predominated, associated or not with signs of bronchitis. the apnea was of ... | 1987 | 2953011 |
| [severe bronchospasm disclosing a respiratory syncytial virus infection in a non-immunocompromised adult]. | 1987 | 2954123 | |
| local and systemic antibody response to bovine respiratory syncytial virus infection and reinfection in calves with and without maternal antibodies. | enzyme-linked immunosorbent assays for the detection of immunoglobulin m (igm), iga, igg1, and igg2 antibodies against bovine respiratory syncytial virus (brsv) were used to measure antibody responses of calves after experimental or natural infection with brsv. serially collected sera, lung lavage samples, nasal and eye secretions, and feces were tested for the presence of these antibodies. lung lavage fluids and nasal secretions were further examined for the presence of virus. after experimenta ... | 1987 | 2954996 |
| effect of ipratropium bromide on respiratory mechanics in infants with acute bronchiolitis. | the effect of nebulized ipratropium bromide in 14 infants (mean age: 20 weeks, range: 4-41) with acute respiratory syncytial virus bronchiolitis was examined. a modified rapid chest compression technique was used to obtain partial expiratory flow-volume curves and maximum flow at functional residual capacity. passive respiratory mechanics were assessed by brief occlusion at end inspiration. thoracic gas volume was measured in a body plethysmograph. no significant difference was found in forced a ... | 1987 | 2959249 |
| [respiratory syncytial virus infection in chronic bronchitis patients]. | two groups of patients with acute respiratory disease (ard) were examined: 57 (the 1st group) had chronic bronchitis, 265 patients (the 2nd group) had no previous history of chronic bronchitis. the investigation was performed using serological and immunofluorescent methods and was repeated in the course of 12-18 months. respiratory syncytial (rs) viral infection was diagnosed significantly more frequently in the 1st group whereas the frequency of influenza, parainfluenza and adenoviral infection ... | 1987 | 2960023 |
| infant pneumonitis associated with cytomegalovirus, chlamydia, pneumocystis, and ureaplasma: follow-up. | a total of 205 infants who were hospitalized when younger than 3 months of age for pneumonitis were followed longitudinally. of these patients, 145 (70%) had evidence of infection with one or more pathogens. the most common etiologic agents were chlamydia trachomatis 61/193 (36%), respiratory syncytial virus 33/142 (23%), cytomegalovirus 42/203 (20%), pneumocystis carinii 30/171 (17%), and ureaplasma urealyticum 21/125 (16%). the initial clinical presentation was characterized by cough, rales, n ... | 1987 | 3025807 |
| immunofluorescence in the serological diagnosis of parainfluenza type 3 and respiratory syncytial virus infection in calves. | the fluorescent antibody (fa) test is compared with the haemagglutination inhibition (hi) test for parainfluenza virus type 3 (pi-3) and virus neutralisation (vn) test for respiratory syncytial (rs) virus for detection and titration of virus-specific antibodies. in experimentally inoculated calves pi-3 and rs virus fa tests detected seroconversion at the same time as hi and vn tests, however, in serially diluted sera, the fa test was positive to higher dilution. in studies with paired samples fr ... | 1986 | 3027802 |
| [rhinovirus and acute respiratory infections in infants]. | human leucocytary interferon (ifn) in a concentration of 2,000 ul/ml stops the development of 9 rhinovirus (rv) strains. the use of serum ifn antibodies (saif) reverses this effect. of 256 nasal aspirates culture negative for other respiratory viruses, 40 rv were isolated with saif as opposed to 19 without serum anti ifn. the frequency and some clinical aspects are emphasized in infants: 43% had bronchiolitis similar to respiratory syncytial virus (rsv) infections and atopy was frequent. | 1986 | 3028312 |
| parainfluenza virus bronchiolitis. epidemiology and pathogenesis. | an investigation of the epidemiology and pathogenesis of bronchiolitis due to parainfluenza virus (pv) was carried out. bronchiolitis due to pv occurred most commonly in non-caucasian males. breast-fed infants exhibited a reduced risk of developing bronchiolitis. once an episode of pv bronchiolitis occurred, both exposure to cigarette smoke and bottle feeding were associated with an increased frequency of recurrent wheezing, and subsequent infection with respiratory virus almost uniformly result ... | 1986 | 3002169 |
| study of prolonged virus infection in cattle stocks infected by bovine respiratory syncytial virus. | 1986 | 2953213 | |
| ribavirin and respiratory syncytial virus. | 1986 | 2868301 | |
| dynamics of some genetic marker changes of respiratory-syncytial virus strains circulating among nursery-school children. | respiratory-syncytial (rs) virus strains circulating during several years appeared polymorphic in respect of two genetic markers: the regression coefficient of infectious activity (rcia39) characterizing the isolates by their reproduction in tissue cultures at supraoptimal temperature (39 degrees c) and the regression coefficient of neutralization indices (rcni) characterizing the degree of sensitivity of the strains to antibodies. high-yield rs viruses were more often isolated from children fre ... | 1986 | 2871732 |
| simultaneous infection with bordetella pertussis and respiratory syncytial virus in hospitalized children. | we compared three groups of hospitalized children with bordetella pertussis infection, respiratory syncytial virus (rsv) infection and dual b. pertussis/rsv infections in an effort to establish clinical and laboratory criteria by which to distinguish children with dual infections from children infected with either organism alone. the groups were compared for admission laboratory data, history of present illness, perinatal history and immunization history. children with pertussis were more likely ... | 1986 | 2876415 |
| complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses. | the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ... | 1986 | 2878059 |
| pneumonia associated with infection with pneumocystis, respiratory syncytial virus, chlamydia, mycoplasma, and cytomegalovirus in children in papua new guinea. | paired serum samples were collected from 94 children with pneumonia admitted to goroka hospital, papua new guinea. all but three of the children were aged 1-24 months. only nine children were malnourished, with weight for age less than 70% of the harvard median (three had weight for age less than 60% of the harvard median). pneumocystis carinii antigen was detected in the serum of 23 children. twenty two children had serological evidence of recent infection with respiratory syncytial virus. five ... | 1986 | 3002538 |
| role of respiratory viruses in exacerbations of primary nephrotic syndrome. | to determine whether respiratory virus infections (uri) are associated with exacerbation of nephrotic syndrome (ns) in childhood, a prospective two-winter study of 32 children with ns was done. we obtained pre- and post-season viral serologic studies, biweekly nose and throat viral cultures, daily urinalysis, biweekly telephone follow-up for uri and renal complaints, and clinical assessments as indicated. when a uri occurred, viral cultures were done weekly if the child was at home and twice wee ... | 1986 | 3005537 |
| serosurvey for selected pathogens in hunter-killed pronghorns in western nebraska. | exposure of pronghorns (antilocapra americana) in western nebraska in 1983 to selected livestock pathogens was examined by serology and attempted virus isolation. antibodies were present to the agents of bluetongue, epizootic hemorrhagic disease, and bovine respiratory syncytial virus. there were no serologic reactors to brucella, and attempts to isolate the viruses of bluetongue and epizootic hemorrhagic disease were negative. | 1986 | 3005667 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| rapid diagnosis of respiratory virus infections in patients with acute respiratory disease. | viral respiratory infections represent a significant segment of the total respiratory disease spectrum; however, until recently the laboratory diagnosis of viral respiratory infections was relatively inefficient. development of new and improved immunologic assay systems has paved the way for accurate and reliable rapid diagnostic tests that detect viral antigens in clinical specimens. we conducted a careful and elaborate study in which radioimmunoassay for antigen detection was compared with a b ... | 1986 | 3009083 |
| simultaneous infections with different enteric and respiratory tract viruses. | infants and young children with rotavirus (rv) or visualized adenovirus in their stools were tested for the simultaneous presence of a respiratory viral pathogen in their upper respiratory tract. overall, at least 10.7% of 484 study subjects had such dual infections, including 8.3% of 385 rv-positive gastroenteritis patients and 24.3% of 37 rv-positive respiratory disease patients. respiratory syncytial virus was present in 34.1% of 41 dual infections with rv and at least 40% of the 12 to 15 dua ... | 1986 | 3009527 |
| evaluation of cell line 293 for virus isolation in routine viral diagnosis. | cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. to explore the possibility of using this cell line for the isolation of other viruses from clinical specimens, 293 cells were tested for their susceptibility to a variety of viruses including herpes simplex virus, parainfluenza viruses, respiratory syncytial virus, and the enter ... | 1986 | 3009540 |
| presence of respiratory viruses in middle ear fluids and nasal wash specimens from children with acute otitis media. | during a 28-month period, 84 children with acute otitis media were studied by viral and bacterial cultures of middle ear fluid and viral cultures of nasal lavage fluid. viruses were isolated from the middle ear fluid of 17 (20%) patients. evidence of viral infection was demonstrated by positive viral cultures of middle ear fluid and/or nasal lavage fluid in 33 (39%) patients. rhinovirus in one patient and influenza b virus in another were the only pathogens isolated. influenza virus, enterovirus ... | 1986 | 3010225 |
| patterns of viral respiratory tract infections in singapore. | a second retrospective study on viral respiratory tract infections, based on 12 years' laboratory reports for the influenza viruses, and 9 years' reports for the other respiratory viruses was made. seasonal variation in incidence was found to occur with infections due to the influenza viruses, respiratory syncytial virus, and parainfluenzavirus type 1. observations made previously on the age distribution of specific respiratory viral infections, and the association of the viruses with various re ... | 1986 | 3010799 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| fever in respiratory virus infections. | the case records of 258 children with adenovirus; influenza a or b virus; parainfluenza 1, 2, or 3 virus; or respiratory syncytial virus infections were studied retrospectively with special attention to the degree and duration of fever. a temperature of 39.0 degrees c or higher was most frequently recorded in adenovirus, influenza a, and influenza b virus infections (in 68%, 84%, and 65%, respectively). the mean highest degree of fever in respiratory virus infections (39.2 degrees c +/- 0.6 degr ... | 1986 | 3020965 |
| ribavirin: a clinical overview. | ribavirin, a broad spectrum, non-interferon-inducing virustatic chemotherapeutic agent, demonstrates activity against a wide range of rna and dna viruses, including the retrovirus known to cause the acquired immune deficiency syndrome. the drug's proposed mechanism of action, as well as pharmacokinetics are discussed, and preclinical toxicity, safety and clinical efficacy studies are presented. to date, the best success has occurred in the use of ribavirin to treat respiratory syncytial virus in ... | 1986 | 3021519 |
| in vitro effect of ascorbic acid on infectivity of herpesviruses and paramyxoviruses. | suspensions of herpes simplex virus types 1 and 2, cytomegalovirus, and parainfluenzavirus type 2 were inactivated within 24 h when treated at 37 degrees c with 1 mg (5.05 mm) of copper-catalyzed sodium ascorbate per ml. the infectivity titer of respiratory syncytial virus was reduced substantially after 24 h but required 48 h for inactivation. under these conditions, inactivation of these viruses was also successfully achieved with 5.68 mm catalyzed ascorbic acid. copper (cu2+), when added with ... | 1986 | 3021808 |
| clinical observations on mechanical ventilation for respiratory failure in bronchiolitis. | an unusually large number of infants (82) were admitted to stanford university hospital from november 1, 1983, through may 31, 1985, with a diagnosis of bronchiolitis requiring oxygen therapy. a larger percentage of these infants (17/82 = 21%) than generally expected required mechanical ventilation for respiratory failure. fourteen infants had respiratory syncytial virus (rsv) infections, and three had parainfluenza virus infections. ten patients had respiratory difficulties as neonates. the mec ... | 1986 | 3022224 |
| nucleotide sequence of the gene encoding the fusion glycoprotein of newcastle disease virus. | the nucleotide sequence of the gene encoding the fusion (f) glycoprotein of the beaudette c strain of newcastle disease virus (ndv) has been determined from cdna clones obtained from virion rna. the gene is 1792 nucleotides long, including mrna start and polyadenylation signals typical of paramyxoviruses. the single open reading frame encodes a polypeptide of 553 amino acids, with a predicted molecular weight of 59042. the f polypeptide has three regions of high hydrophobicity: an n-terminal sig ... | 1986 | 3025345 |
| antigen mimicry involving measles virus hemagglutinin and human respiratory syncytial virus nucleoprotein. | intergenic antigenic relationships between measles virus and respiratory syncytial (rs) virus-specific structural components were studied by using monoclonal antibodies. of 75 monoclonal antibodies against these components, only one, an anti-measles virus hemagglutinin monoclonal antibody, cross-reacted. immunofluorescence analysis of measles virus- and rs virus-infected cells with this monoclonal antibody showed qualitatively different staining patterns which indicated that the antigen involved ... | 1986 | 2416956 |
| respiratory syncytial virus glycoprotein expression in human and simian cell lines. | glycoproteins synthesized in both human (hela and hep-2) and simian (vero and bs-c-1) cell lines following infection with two different strains of respiratory syncytial virus (a2 and long) were analysed by sds-page following immunoprecipitation with monoclonal antibodies. minor virus strain-dependent differences in the large glycoprotein, g, and the fusion protein polypeptides f1 and f2 were observed together with minor cell line-dependent differences in the size of the f2 polypeptide. major qua ... | 1986 | 2427648 |
| human and murine cytotoxic t cells specific to respiratory syncytial virus recognize the viral nucleoprotein (n), but not the major glycoprotein (g), expressed by vaccinia virus recombinants. | the viral antigens recognized by cytotoxic t cells (ctl) have not been defined in most viruses infecting mouse or man. natural or artificial virus recombinants can be used to determine the antigen specificity of ctl directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. we describe here the use of recombinant vaccinia viruses, containing cdna corresponding to either the nucleoprotein (n) gene or the m ... | 1986 | 2431051 |
| interferon-mediated self-limiting growth of respiratory syncytial virus in mouse embryo cells. | the growth of respiratory syncytial (rs) virus in primary mouse embryo (me) cells was investigated. the virus yields in me cells were markedly lower if compared with those in hep-2 cells, which are fully permissive for rs virus, and a remarkable production of interferon (ifn) was found in the early period of infection of the former cells. the virus yields in me cells were enhanced when antimouse ifn serum was added to the medium. indirect immunofluorescent staining of infected me cells showed th ... | 1986 | 2432168 |
| respiratory syncytial virus--oklahoma. | 1986 | 3081783 | |
| evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions. | a commercially-available direct immunofluorescence (if) reagent (imagen; boots-celltech, slough, berkshire, united kingdom) was similar in sensitivity and specificity to the conventional indirect if test for the detection of respiratory syncytial virus in respiratory secretions. both if tests were more sensitive than culture, particularly for specimens transported from outside the institution. | 1986 | 3088032 |
| specific human cytotoxic t cells recognize b-cell lines persistently infected with respiratory syncytial virus. | the t-lymphocyte response to respiratory syncytial (rs) virus has been invoked to explain the bronchiolitis and pneumonia caused by rs virus in human infants. however, t cells also appear to play a role in protection against rs virus infection. although rs virus-specific human lymphocytes have been demonstrated, neither the phenotype nor the function of the lymphocytes was characterized. we describe here the induction of anti-rs virus cytotoxic t lymphocytes, in both bulk culture and restimulate ... | 1986 | 3097646 |
| respiratory syncytial virus infection in hospitalized children over one year of age. | 1986 | 3110028 | |
| rs virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay. | two techniques for rapid diagnosis, immunofluorescence (ifat) and enzyme immunoassay (eia), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (rsv) in specimens of nasopharyngeal secretions. the specimens were obtained from children under five years of age suffering from acute respiratory illness, during a period of six months from january to june 1982. of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) w ... | 1986 | 3295470 |
| serologic survey of selected pathogens in white-tailed and mule deer in western nebraska. | exposure of free-ranging white-tailed deer (odocoileus virginianus) and mule deer (odocoileus hemionus) in western nebraska to selected livestock pathogens was determined by serology and attempted virus isolation. antibodies to bluetongue virus, epizootic hemorrhagic disease virus, and bovine respiratory syncytial virus were present in both species of deer. no serologic reactors to brucella or anaplasma were found. attempts to isolate bluetongue virus were negative. | 1986 | 2845156 |
| comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. | we compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (rsv) antigens in an indirect enzyme-linked immunosorbent assay (elisa). of 28 infants positive for rsv by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by elisa with whole nasal secretions, whereas only 19 (68%) were positive by elisa with the matching washed-cell fractions. furthermore, the elisa absorbances obtained with nasal secretions were ... | 1986 | 3700621 |
| enhanced isolation of respiratory syncytial virus in cell culture. | during two winter seasons, we found that the combination of wi-38 or mrc-5 human lung fibroblasts plus primary rhesus monkey kidney (rhmk) and hep-2 cell cultures yielded maximal isolation of respiratory syncytial virus. cytopathic effects (cpe) developed earliest in rhmk cells and slowest in the human fibroblast lines. in rhmk cells, 50% of ultimately positive cultures showed cpe in 5 days, and 90% of positive cultures showed cpe within 7 days during both respiratory syncytial virus seasons. | 1986 | 3700634 |
| measles virus rna detected in paget's disease bone tissue by in situ hybridization. | morphological and immunocytological studies have demonstrated the presence of paramyxovirus antigens in paget's bone disease tissue and in particular antigens related to measles virus and respiratory syncytial virus. to examine the relationship between measles virus and paget's bone disease we used in situ hybridization and a cloned measles virus dna probe specific for the nucleocapsid protein to detect and locate measles virus rna sequences in paget's bone tissue. in five patients with the dise ... | 1986 | 3701300 |
| risk of primary infection and reinfection with respiratory syncytial virus. | respiratory syncytial virus is the most important cause of serious lower respiratory tract infection in children. for children followed up from birth in the houston family study, the infection rate was 68.8/100 children less than 12 months of age and 82.6/100 during the second year of life. virtually all children had been infected at least once by 24 months of age, and about one half had experienced two infections. although lower respiratory tract disease (lrd) was common (22.4/100 during year 1 ... | 1986 | 3706232 |
| murine pneumonia virus: seroepidemiological evidence of widespread human infection. | more than 75% of a random sample of adult human sera exhibited moderate to high murine pneumonia virus (pvm)-neutralizing activity. there was no correlation between pvm-neutralizing activity and respiratory syncytial virus or parainfluenza type 3 virus-neutralizing activities of the same sera. in children the proportion of sera with moderate to high titres increased with age, indicating early exposure to infection. seroconversion (i.e. greater than fourfold increase in titre) was observed in fou ... | 1986 | 3711866 |
| the use of purified immunoglobulin in the therapy of respiratory syncytial virus infection. | 1986 | 3714524 | |
| [respiratory syncytial virus infections in newborn infants]. | twenty-three of 23 neonates were contaminated in the course of an outbreak of respiratory syncytial virus (rsv) in a neonatal care unit. symptoms among 22 infected symptomatic infants included rhinitis (n = 21), dyspnea (n = 19), cough (n = 17), apnea (n = 5), seizures (n = 3), fever (n = 3). five patients presented with severe respiratory distress. the occurrence of non-obstructive apnea was significantly correlated with a history of respiratory disease, rsv infection during the first 15 days o ... | 1986 | 3718165 |
| culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers. | in preliminary studies, a system for the propagation of respiratory syncytial virus (rsv) using cytodex 3 microcarriers was established. optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/l with a cell inoculum density of 4 x 10(4) cells/ml tissue culture medium. under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. maximum yield of virus antigen was achiev ... | 1986 | 3718237 |
| respiratory syncytial viral infection in children with compromised immune function. | for 10 winters, 608 children five years old or younger who were hospitalized with respiratory syncytial virus (rsv) infection were prospectively studied to evaluate the relation between their immune status and the severity of their infection. forty-seven had been immunocompromised by chemotherapy, steroid therapy, or a primary immunodeficiency disorder. among the immunocompromised children, those receiving chemotherapy for cancer and those with immunodeficiency disease had more severe rsv diseas ... | 1986 | 3724802 |
| atypical lymphocytosis in respiratory syncytial virus pneumonia. | 1986 | 3725666 | |
| [sensitivity of cell cultures to the bovine respiratory syncytial virus]. | investigated was the individual susceptibility of individual batches of primary cell cultures of fetal and normal calf kidney as well as of individual series of cultures of fetal and normal lamb kidney to the replication of the strains antonovo/448 and nomi of the bovine respiratory-syncytial virus. it was found that both types of primary cultures possessed varying susceptibility to the virus, which was likely to be genetically substantiated. it was shown that beside such susceptibility of vario ... | 1986 | 3727375 |
| value of serological tests in the diagnosis of viral acute respiratory infections in adults. | the dynamics of the antibody response to influenza viruses a (h1n1), a (h3n2) and b, to parainfluenza viruses 1, 2, 3, to adenoviruses and respiratory syncytial virus was studied in paired serum samples collected from 110 patients hospitalized with acute respiratory infections (ari) and in 40 patients suffering from other diseases. rises in serum antibody titers to 1--5 of the above mentioned antigens were detected in many of the patients of both groups. the fact is most likely due to the presen ... | 1986 | 3727398 |
| [predictive indicators of the severity of bronchiolitis caused by respiratory syncytial virus in infants]. | 1986 | 3729251 | |
| study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
| serologic evidence of respiratory syncytial virus infection in free-ranging mountain goats (oreamnos americanus). | 1986 | 3735589 | |
| isolation and serologic evidence of a respiratory syncytial virus in bighorn sheep from colorado. | 1986 | 3735590 | |
| respiratory syncytial virus: a report of a 5-year study at a children's hospital. | a study over a 5-year period (1979-1983) of rsv infections in children in sydney, australia is reported. in common with findings made elsewhere in the world, annual epidemics of rsv infection commencing in autumn and lasting 4-6 months, with peak activity in mid-winter, were observed in 1979, 1980, and 1983. however, in 1981 and 1982 virus activity was first detected in midsummer, peaked in autumn, and was present throughout most months of the year. the alteration in virus activity in 1981 and 1 ... | 1986 | 3746259 |
| clenbuterol hydrochloride in calves with a natural bovine respiratory syncytial virus infection. | the effect of clenbuterol hydrochloride on the course of disease in calves with a natural bovine respiratory syncytial virus infection was examined. six calves (three to nine months of age) originating from four herds with respiratory tract disease and serological evidence of a bovine respiratory syncytial virus infection were used in this study. the calves were injected intravenously with clenbuterol hydrochloride. the effect of clenbuterol on the course of disease was measured using the po2 in ... | 1986 | 3750791 |