Publications

biodehalogenation: reactions of cytochrome p-450 with polyhalomethanes.	the products, stoichiometry, and kinetics of the oxidation of the enzyme cytochrome p-450 cam by five polyhalomethanes and chloronitromethane are described. the reactivity of the enzyme is compared with that of deuteroheme and with the enzyme in its native cell, pseudomonas putida (ppg-786). in all cases, the reaction entails hydrogenolysis of the carbon-halogen bond: 2feiip + rcxn----2feiiip + rchxn-1 (p = porphyrin or p-450 cam in vitro and in vivo). trichloronitromethane was the fastest react ...	1985	4039602
purification and some properties of two isofunctional juglone hydroxylases from pseudomonas putida j1.	juglone-induced cells of pseudomonas putida j 1 were shown to contain two isofunctional juglone hydroxylases. both enzymes were purified about 125-fold to homogeneity in polyacrylamide gel electrophoresis. the molecular masses of the native enzymes, as determined by sephacryl s-200 gel filtration were 59 000 da for enzyme 1 and 56 000 da for enzyme 2. the molecular masses of the subunits were determined by dodecyl sulfate polyacrylamide gel electrophoresis as 25 000 da (enzyme 1) and 23 500 da ( ...	1985	4041238
[stability of the npl-1 and npl-41 plasmids of naphthalene biodegradation in pseudomonas putida populations in continuous culture].	the stability of biodegradation plasmids npl-1 and npl-41, which control the synthesis of enzymes for naphthalene oxidation to salicylate, was studied in pseudomonas putida bsa under the conditions of its continuous cultivation with limitation in glucose or salicylate in the chemostat regime and without limitation in the ph-stat regime. plasmid npl-1, which controls the inducible synthesis of naphthalene oxygenase, is stable in the population of p. putida cells under the conditions of continuous ...	1985	4058326
[glucose consumption and dehydrogenase activity of the cells of the arsenite-oxidizing bacterium pseudomonas putida].	the rates of glucose assimilation and dehydrogenase activity were studied in pseudomonas putida oxidizing arsenite. the rate of glucose utilization by the cells decreased in the presence of arsenites in the medium at the beginning because of the microbial adaptation to arsenite. the activity of dehydrogenase fell down when the cells were cultivated in the medium with arsenite. an inverse correlation existed between the rate of glucose assimilation and arsenite oxidation. apparently, arsenites we ...	1985	4058329
formation and properties of flavoprotein-cytochrome hybrids by recombination of subunits from different species.	p-cresol methylhydroxylases from four different pseudomonads differ in their isoelectric points and, to a lesser extent, in mr values and substrate specificity. the enzymes from three species were isolated in homogeneous form, then resolved into their flavoprotein and cytochrome subunits, and the subunits were recombined to yield the nine possible hybrids (i.e. three intraspecies and six interspecies). the resulting flavocytochromes showed extensive similarities in steady-state kinetic parameter ...	1985	4062904
degradation of lawsone by pseudomonas putida l2.	from humus obtained from stuttgart, a bacterium was isolated with lawsone (2-hydroxy-1,4-naphthoquinone) as selective source of carbon. this bacterium is capable of utilizing lawsone as sole source of carbon and energy. morphological and physiological characteristics of the bacterium were examined and it was identified as a strain of pseudomonas putida. the organism is referred to as pseudomonas putida l2. the degradation of lawsone by pseudomonas putida l2 was investigated. salicylic acid and c ...	1985	4063065
in vivo role of sulfite in photocontrol of urocanase from pseudomonas putida.		1985	2858892
activation of urocanase from pseudomonas putida by electronically excited triplet species.	urocanase from pseudomonas putida becomes inactive in growing and resting cells and, as shown previously, is activated by the direct absorption of ultraviolet light. in this study, we describe the activation of urocanase by energy transfer from triplet indole-3-aldehyde, generated in the peroxidase-catalyzed aerobic oxidation of indole-3-acetic acid. the activation was time-, temperature-, and ph-dependent. the involvement of reactive oxygen intermediates was excluded by the lack of effect of ap ...	1985	2864338
tryptophanyl fluorescence quenching of urocanase from pseudomonas putida by acrylamide, cesium, iodide, and imidazolepropionate.		1985	2864711
cloning and expression in escherichia coli of histidine utilization genes from pseudomonas putida.	a library of the pseudomonas putida chromosome, prepared through the use of the cosmid pjb8 ligated to a partial sau3a digest of bacterial dna, followed by in vitro packaging into bacteriophage lambda particles, was used to construct a strain of escherichia coli which contained the genes for histidine utilization. this isolate produced a repressor product and all five enzymes required in pseudomonas spp. for histidine dissimilation, whereas none of these could be detected in the nontransduced pa ...	1985	2858467
omega mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species.	we have used the 2.0-kb dna fragment omega [prentki and krisch, gene 29 (1984) 303-313] to mutagenize in vitro a broad-host-range plasmid carrying the entire meta-cleavage pathway of the pseudomonas putida tol plasmid pww0. the mutant plasmids were subsequently introduced by conjugal mobilization into a variety of gram-negative bacteria. the omega fragment carries a selectable marker (aada+; spcr/smr), which is expressed in all species tested, as well as flanking transcription and translation te ...	1985	2998930
[expression of pseudomonas aeruginosa transposable phages in pseudomonas putida cells. i. the establishment of a lysogenic state and the effectiveness of lytic development].	expression of transposable phages (tp) of pseudomonas aeruginosa in the cells of p. putida was studied. the high efficiency of phage lytic development was shown both as a consequence of zygotic induction after transfer of the rp4::tpc+ plasmid into nonlysogenic recipients, and as a result of heat induction of lysogens ppg1 (d3112cts15). the high phage yield (20-25 particles of d3112cts phage per one cell of p. putida) is an evidence for a high level of transposition in the cells of this bacteria ...	1985	2998927
[17o]water and nitric oxide binding by protocatechuate 4,5-dioxygenase and catechol 2,3-dioxygenase. evidence for binding of exogenous ligands to the active site fe2+ of extradiol dioxygenases.	pseudomonas testosteroni protocatechuate 4,5-dioxygenase and pseudomonas putida catechol 2,3-dioxygenase (metapyrocatechase) catalyze extradiol-type oxygenolytic cleavage of the aromatic ring of their substrates. the essential active site fe2+ of each enzyme binds nitric oxide (no) to produce an epr active complex with an electronic spin of s = 3/2. hyperfine broadening of the epr resonances of the nitrosyl complexes by 17o-enriched h2o shows that water is bound directly to the fe2+ in the nativ ...	1985	2997190
evolutionary conservation of genes coding for meta pathway enzymes within tol plasmids pww0 and pww53.	pseudomonas putida mt53 contains a tol plasmid, pww53, that encodes toluene-xylene catabolism. pww53 is nonconjugative, is about 105 to 110 kilobase pairs (kbp) in size, and differs significantly in its restriction endonuclease digestion pattern and incompatibility group from the archetypal tol plasmid pww0. an rp4::pww53 cointegrate plasmid, pww53-4, containing about 35 kbp of pww53 dna, including the entire catabolic pathway genes, was formed, and a restriction map for kpni, hindiii, and bamhi ...	1985	2997136
determination of the transcription initiation site and identification of the protein product of the regulatory gene xylr for xyl operons on the tol plasmid.	the xylr gene is a regulatory gene on the tol plasmid, which acts in a positive manner on xyl operons for degradation of toluene and xylenes in pseudomonas putida. a dna fragment containing the xylr promoter region was cloned on promoter-probing vectors, and its nucleotide sequence was determined. the transcription initiation site of the xylr gene was determined in cells of p. putida and escherichia coli by s1 nuclease and reverse transcriptase mapping. two initiation sites were detected which w ...	1985	2993247
the site of cyclic amp-dependent protein kinase catalyzed phosphorylation of cytochrome p-450 lm2.	the phenobarbital-inducible form of cytochrome p-450 purified from rabbit liver microsomes is phosphorylated by camp-dependent protein kinase at a single site, the serine residue in position 128 of the amino acid sequence. the serine is located in a characteristic recognition sequence for camp-dependent protein kinase and is part of a primary structure which is conserved during evolution, present also in phenobarbital-inducible rat cytochrome and cytochrome p-450 cam from pseudomonas putida. the ...	1985	2991008
[expression of pseudomonas aeruginosa phage transposons in pseudomonas putida ppg1 cells. ii. zygotic induction--a necessary condition for the formation of defective lysogens].	the transfer of hybrid plasmid rp4::pt (where pt is the genome of a transposable phage specific for pseudomonas aeruginosa) into recipient cells of p. putida strain ppg1 occurs with the same frequency as into p. aeruginosa, the homologous host for pt. approximately 1/3 of all ppg1 exconjugants carrying rp4 markers lost the capability to produce viable pt phage. in contrast, in a cross with homologous recipient p. aeruginosa all exconjugant clones contained nondefective prophages in the hybrid pl ...	1985	3002910
[hybrid plasmid pbs251 containing genes for n-alkane degradation].	the strain of pseudomonas aeruginosa bs316 utilizing h-alkanes of the c6-c12 series (alk+) harbours the 96 md plasmid pbs250. the use of plasmid rp4 to mobilize alk+ markers in conjugal transfer to pseudomonas aeruginosa and pseudomonas putida has resulted in isolation of transconjugants resistant to antibiotics (due to genes coded by plasmid rp4) and capable of growth on h-alkanes. a transconjugant from this series harbours plasmid pbs251, a derivative of plasmid rp4 containing the genes for oc ...	1985	3025683
cloning of the gene for the common pathogenic neisseria h.8 antigen from neisseria gonorrhoeae.	neisseria gonorrhoeae dna that encodes the pathogenic neisseria h.8 common antigen was cloned in the lambda phage sep6. the recombinant phage, designated s6h.8, was detected immunologically with a monoclonal antibody that binds to the h.8 antigen. the gonococcal and s6h.8 forms of the antigen yielded identical partial proteolysis epitope maps. neisseria species that did not manifest the h.8 antigen showed little or no dna homology with s6h.8. this clone should facilitate investigation into the c ...	1985	2981198
purification and properties of ferredoxintol. a component of toluene dioxygenase from pseudomonas putida f1.	toluene dioxygenase oxidizes toluene to (+)-cis-1(s),2(r)-dihydroxy-3-methylcyclohexa-3,5-diene. this reaction is catalyzed by a multienzyme system that is induced in cells of pseudomonas putida f1 during growth on toluene. one of the components of toluene dioxygenase has been purified to homogeneity and shown to be an iron-sulfur protein that has been designated ferredoxintol. the molecular weight of ferredoxintol was calculated to be 15,300, and the purified protein was shown to contain 2 g of ...	1985	2982815
sal-tol in vivo recombinant plasmid pkf439.	sal-tol in vivo recombinant plasmid pkf439 was characterized in a strain from a mixed culture of bacteria harboring various degradative plasmids. analysis of the gene organization of pkf439 revealed that the 57-kilobase tol fragment, including the 40-kilobase tol metabolic region, was inserted into the complete sal replicon at the position of smai-c within xhoi-b of sal. the molecular size of pkf439 was calculated to be 138 kilobases. pkf439 could be transferred to pseudomonas putida and pseudom ...	1985	2987190
shuttle vector for escherichia coli, pseudomonas putida, and pseudomonas aeruginosa.	a hybrid plasmid capable of replication in 2 different genera, escherichia and pseudomonas, was constructed. this plasmid dna can be used as a cloning vector in e. coli and pseudomonades cells. the described hybrid plasmid pld411 has been constructed on the basis of 2 small e. coli vector r-plasmids used in our laboratory and cryptic plasmid pww2 or p. putida mt1. plasmid pld411 dna was mapped with restrictases; its biological activity in transformations of different bacterial strains was studie ...	1985	2990432
p-cresol methylhydroxylase. assay and general properties.	p-cresol methylhydroxylase from pseudomonas putida, an anaerobic dehydrogenase that catalyses the oxidation of p-cresol to p-hydroxybenzyl alcohol and then to p-hydroxybenzaldehyde, is an enzyme of great interest in several respects. one of these is the fact that its flavoprotein and cytochrome c subunits may be reversibly dissociated with ease, with full regeneration of the activity and its native properties on recombining the components. bisubstrate kinetic analysis of the unresolved enzyme gi ...	1985	2990444
p450cam gene cloning and expression in pseudomonas putida and escherichia coli.	the gene camc, which encodes the cytochrome p450 monoxygenase protein, was cloned into the shuttle vector pkt240 and recovered as the recombinant pkg201 with a 2.3 kb insert from the cam plasmid in the psti site. the gene product is expressed constitutively in p. putida and in e. coli whereas the inverted insert clone lacks expression, indicating absence of an insert promoter.	1985	2992468
transposon donor plasmids, based on colib-p9, for use in pseudomonas putida and a variety of other gram negative bacteria.	the properties of plg221, a derivative of the colib plasmid carrying the transposon tn5 are described. this plasmid can be used to introduce tn5 by conjugation from escherichia coli into a variety of gram negative bacteria outside the host range for maintenance of colib. plasmid plg221, and a similar plasmid plg223 carrying tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of gram negative bacteria.	1985	2993815
isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating pseudomonas putida wcs358.	the plant-growth-stimulating pseudomonas putida wcs358 was mutagenized with transposon tn5. the resulting mutant colony bank was screened for mutants defective in the biosynthesis of the fluorescent siderophore. a total of 28 mutants, divided into six different classes, were isolated that were nonfluorescent or defective in iron acquisition or both. these different types of mutants together with the probable overall structure of the siderophore, i.e., a small peptide chain attached to a fluoresc ...	1985	2997118
dehalogenase genes of pseudomonas putida pp3 on chromosomally located transposable elements.	pseudomonas putida pp3 utilizes halogenated alkanoic acids (haa) such as 2,2-dcpa as its sole carbon and energy sources. spontaneous hha- mutants, isolated by selection for resistance to the toxic analogs monochloroacetic acid and dichloroacetic acid, arose at frequencies several orders of magnitude higher than expected for spontaneous mutations. analysis of the five classes of mutants isolated suggested that the dehalogenase and haa permease genes were on chromosomally located transposable elem ...	1985	2835577
expression of plasmid encoded escherichia coli 5s ribosomal ribonucleic acid in pseudomonas putida.	the recombinant plasmid pnrk 36, which represents the plasmids rsf 1010, a small multicopy plasmid of the incompatibility group incq that confers resistance to streptomycin and sulfoamide to its host cells, and pkk 223-3, which contains the try-lac (tac) promoter followed by a polylinker and a dna segment containing the 5s rrna (rrn b) with the ribosomal rna transcription terminators, was employed to transform pseudomonas putida 2440 cells. the plasmid encoded 5 s rrna from escherichia coli was ...	1985	2411598
the kinetics of dihydrostreptomycin uptake in pseudomonas putida membrane vesicles: absence of inhibition by cations.	dihydrostreptomycin was taken up in isolated cytoplasmic membrane vesicles of pseudomonas putida by an active transport mechanism. saturation kinetics were observed with an apparent km and vmax of 15 mm and 50 nmol/min/mg of protein respectively. the evidence suggested that the observed kinetics was that of the energy-dependent phase i component of dihydrostreptomycin uptake. neither magnesium nor the polyamine, spermine, inhibited dihydrostreptomycin transport. thus, the inhibition of aminoglyc ...	1985	2415504
characterization of two surface-localized antigenic sites on porin protein f of pseudomonas aeruginosa.	a rapid colony immunoblot screening procedure was used to demonstrate the surface localization of porin protein f on bacterial colonies of pseudomonas aeruginosa. by this method, we demonstrated that protein f was accessible to four different specific monoclonal antibodies in a wide variety of both mucoid and nonmucoid p. aeruginosa strains. controls were performed to demonstrate that, using this procedure, only surface-exposed epitopes bound monoclonal antibodies and that nonspecific binding of ...	1985	2408719
interaction of pseudomonas putida atcc 12633 and bacteriophage gh-1 in berea sandstone rock.	measurements of the passage of pseudomonas putida atcc 12633 and a phage-resistant mutant through berea sandstone rock were made. when bacteriophage gh-1 was adsorbed within the rock matrix, a reduction in the passage of the susceptible but not the resistant cells through the rock was observed.	1985	16346956
characterization of bacteria by particle beam mass spectrometry.	a technique is described for detecting and characterizing bacteria on a single-particle basis by mass spectrometry. the method involves generation of a particle beam of single whole cells which are rapidly volatilized and ionized in vacuum in the ion source of a quadrupole mass spectrometer. the particle beam can be generated, with minimal sample handling, from a naturally occurring aerosol or from a solution of bacteria that can be dispersed as an aerosol. the mass spectrum is generated by succ ...	1985	16346802
localization of polyvinyl alcohol oxidase produced by a bacterial symbiont, pseudomonas sp. strain vm15c.	an axenic culture of a polyvinyl alcohol (pva)-degrading symbiont, pseudomonas sp. strain vm15c, was established on pva with a crude preparation of the growth factor (factor a) produced by the symbiotic partner pseudomonas putida vm15a. an increase of factor a in the culture medium enhanced the cell-associated pva oxidase activity as well as the growth rate, but decreased production of extracellular pva oxidase. pva oxidase in cells grown on pva was present in the periplasmic space at a higher r ...	1985	16346711
enhancement of pyrroloquinoline quinone production and polyvinyl alcohol degradation in mixed continuous cultures of pseudomonas putida vm15a and pseudomonas sp. strain vm15c with mixed carbon sources.	in a mixed continuous culture of pseudomonas putida vm15a and pseudomonas sp. strain vm15c with polyvinyl alcohol (pva) as the sole source of carbon, growth of the pva-degrading bacterium vm15c and, hence, pva degradation were limited by the growth factor, pyrroloquinoline quinone, produced by vm15a. feeding of a carbon source for vm15a, ethanol, with pva enhanced pyrroloquinoline quinone production and caused increases in the vm15c population and pva degradation in a mixed continuous culture. t ...	1985	16346804
formation of filaments by pseudomonas putida.	when pseudomonas putida 40 was grown on a variety of liquid media in which oxygen became a limiting factor during growth, the latter stages of growth involved the elongation of cells without septation, which can result in the complete filamentation of the culture (up to several hundred micrometers long). the filaments appeared to consist of a chain of protoplasts within a common sacculus. later these filaments were capable of a rapid fragmentation by septation to give a population of ordinary ro ...	1985	16346856
population dynamics of soil pseudomonads in the rhizosphere of potato (solanum tuberosum l.).	rhizosphere population dynamics of seven pseudomonas fluorescens and pseudomonas putida strains isolated from rhizospheres of various agricultural plants were studied on potato (solanum tuberosum l.) in field soil under controlled environmental conditions. rhizosphere populations of two strains (b10 and b4) were quantitatively related to initial seed piece inoculum levels when plants were grown at -0.3 bar matric potential. at a given inoculum level, rhizosphere populations of strain b4 were con ...	1985	16346729
sand administration as an instrument for biofilm control of pseudomonas putida atcc 11172 in chemostat cultures.	pseudomonas putida atcc 11172 was grown in chemostat on l-asparagine or phenol as the sole, limiting carbon and energy source. the growth characteristics of a culture where a biofilm was present, were compared with one where the biofilm was strongly reduced by the grinding and shearing effect of sand suspended in the culture. in the presence of the intact biofilm, the curve of steady-state biomass versus dilution rate diverged greatly from the theoretical pattern predicted by conventional chemos ...	1985	18553582
acetate inhibition of pseudomonas putida.	to study the effect of acetate inhibition on the parameters of yield and maintenance for bacterial growth, pseudomonas putida atcc 23467 was grown in a minimal salts medium with acetate as the sole carbon source with limiting and with excess quantities of urea in the feed medium. the behavior of the chemostat cultures under sole acetate limitation results in low residual acetate present in the fermentation broth. these cultures can be described satisfactorily using the equation q(s) = d/y(g) + m ...	1985	18553826
cadmium-resistant pseudomonas putida synthesizes novel cadmium proteins.	three cysteine-rich proteins of molecular weight 4000 to 7000, containing 4 to 7 gram atoms of cadmium, zinc, and copper per mole were isolated from pseudomonas putida growing in 3 mm cadmium. the three proteins were induced during different phases of growth, and the smallest (molecular weight 3600; 3 gram atoms of cadmium) was released into the medium when the cells lysed. the results of amino acid analyses and of ultraviolet, circular dichroism, electron paramagnetic resonance, and cadmium-113 ...	1984	17783048
mixed continuous cultures of polyvinyl alcohol-utilizing symbionts pseudomonas putida vm15a and pseudomonas sp. strain vm15c.	stable mixed continuous cultures of pseudomonas sp. strain vm15c and pseudomonas putida vm15a, the former of which produced a polyvinyl alcohol (pva)-degrading enzyme and the latter of which produced an essential growth factor for pva utilization by strain vm15c, were established with pva as the sole source of carbon and energy with chemostat cultivation. a high extent of pva degradation was achieved at dilution rates of less than 0.030/h. the predominant strain in the cultures was the primary m ...	1984	16346642
suicide inactivation of catechol 2,3-dioxygenase from pseudomonas putida mt-2 by 3-halocatechols.	the inactivation of catechol 2,3-dioxygenase from pseudomonas putida mt-2 by 3-chloro- and 3-fluorocatechol and the iron-chelating agent tiron (catechol-3,5-disulfonate) was studied. whereas inactivation by tiron is an oxygen-independent and mostly reversible process, inactivation by the 3-halocatechols was only observed in the presence of oxygen and was largely irreversible. the rate constants for inactivation (k(2)) were 1.62 x 10 sec for 3-chlorocatechol and 2.38 x 10 sec for 3-fluorocatechol ...	1984	16346490
microbial transformation of esters of chlorinated carboxylic acids.	two groups of compounds were selected for microbial transformation studies. in the first group were carboxylic acid esters having a fixed aromatic moiety and an increasing length of the alkyl component. ethyl esters of chlorine-substituted carboxylic acids were in the second group. microorganisms from environmental waters and a pure culture of pseudomonas putida u were used. the bacterial populations were monitored by plate counts, and disappearance of the parent compound was followed by gas-liq ...	1984	16346459
transcription of the tol plasmid toluate catabolic pathway operon of pseudomonas putida is determined by a pair of co-ordinately and positively regulated overlapping promoters.	expression of the meta-cleavage pathway operon of tol plasmid pww0 of pseudomonas putida is positively regulated by the xyls gene product. we have sequenced the promoter region of this operon and localized the transcription initiation sites. two overlapping promoters, designated pm1 and pm2, are responsible for the positively regulated expression of the meta-pathway operon. mutants of p. putida were isolated that expressed the meta-cleavage pathway operon constitutively. several plasmid-located ...	1984	6096122
[introduction of the hybrid plasmid rp4::d3112 into pseudomonas putida cells requires the presence of specific mutation in the phage genome].	the wild type of d3112, a transposable phage of pseudomonas aeruginosa can not be introduced as a portion of the hybrid plasmid rp4::d3112 into pseudomonas putida cells. it is only possible when phage d3112 carries mutations designated lpc (lethal for p. putida and escherichia coli). analysis of heteroduplex molecules between dnas of phages d3112w+ and d3112lpc demonstrated the absence of nonhomology regions, which suggests that lpc is a point mutation. the lpc2 mutation was located within the i ...	1984	6086454
stereochemistry of 1-(4'-hydroxyphenyl)ethanol produced by hydroxylation of 4-ethylphenol by p-cresol methylhydroxylase.	enzymic hydroxylation of 4-ethylphenol by (a) pseudomonas putida and (b) highly purified p-cresol methylhydroxylase gave optically active 1-(4'-hydroxyphenyl)-ethanol. the products were transformed into the phenolic methyl ethers and shown to contain 69.5% and 65.6%, respectively, of the (s)-(-)-isomer. the stereochemistry of the reaction is discussed in terms of three distinct steps occurring at the active site of the enzyme.	1984	6083780
pseudomonas putida in transfused blood.		1984	6145996
sequence of the small subunit of yeast carbamyl phosphate synthetase and identification of its catalytic domain.	the yeast gene cpa1 coding for the small subunit of arginine-specific carbamyl phosphate synthetase has been cloned by complementation of a cpa 1 mutant with a plasmid library of total yeast chromosomal dna. two of the plasmids, pjl113/st4 and pjl113/st15, contain dna inserts in opposite orientations with overlapping sequences of 2.6 kilobases. the nucleotide sequence of a 2.2-kilobase region of the dna insert carrying the cpa1 gene has been determined. the cpa1 gene has been identified to be 12 ...	1984	6086650
transposon mutagenesis analysis of meta-cleavage pathway operon genes of the tol plasmid of pseudomonas putida mt-2.	hybrid plasmids containing the regulated meta-cleavage pathway operon of tol plasmid pwwo were mutagenized with transposon tn1000 or tn5. the resulting insertion mutant plasmids were examined for their ability to express eight of the catabolic enzymes in escherichia coli. the physical locations of the insertions in each of 28 tn1000 and 5 tn5 derivative plasmids were determined by restriction endonuclease cleavage analysis. this information permitted the construction of a precise physical and ge ...	1984	6090417
genetic and physical analyses of caulobacter crescentus trp genes.	caulobacter crescentus trp mutants were identified from a collection of auxotrophs. precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpa, trpb, trpc, trpd, trpe, and trpf. genetic mapping experiments demonstrated that the trp genes were in two clusters, trpcde and trpfba, and a 5.4-kilobase restriction fragment from the c. crescentus chromosome was isolated that contained the trpfba gene cluster. co ...	1984	6090420
nucleotide sequence of the promoter region of the xyldegf operon on tol plasmid of pseudomonas putida.	the transcription initiation site of the xyldegf operon on the tol plasmid of pseudomonas putida mt-2 was determined in p. putida and in escherichia coli by s1 nuclease and reverse transcriptase mapping. the induced synthesis of mrna started at the same start point in both p. putida and e. coli, although the amount of mrna in e. coli cells was less than that in p. putida. the nucleotide sequence of the region surrounding the start point was also determined. the ribosome-binding site (rbs) comple ...	1984	6092237
novel system for recognizing and eliminating foreign dna in pseudomonas putida.	derivatives of pqsr49 (r1162::tn1) containing cloned fragments of escherichia coli chromosomal dna are stable in e. coli but unstable in pseudomonas putida. similar derivatives containing p. putida chromosomal dna are stable in both species. instability is a consequence of plasmid loss during growth and is not due to death or inhibition of growth of plasmid-containing cells. average copy numbers per cell of the unstable hybrid plasmids are similar to that of pqsr49, indicating that instability i ...	1984	6086582
attachment of diaminopimelic acid to bdelloplast peptidoglycan during intraperiplasmic growth of bdellovibrio bacteriovorus 109j.	an early event in the predatory lifestyle of bdellovibrio bacteriovorus 109j is the attachment of diaminopimelic acid (dap) to the peptidoglycan of its prey. attachment occurs over the first 60 min of the growth cycle and is mediated by an extracellular activity(s) produced by the bdellovibrio. some 40,000 dap residues are incorporated into the escherichia coli bdelloplast wall, amounting to ca. 2 to 3% of the total initial dap content of its prey cells. incorporation of dap occurs when e. coli, ...	1984	6202674
enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by pseudomonas sp. strain b13.	dna fragments containing the xyld and xyll genes of tol plasmid pww0 -161 of pseudomonas putida, which code for the catabolic enzymes toluate 1,2-dioxygenase and dihydrodihydroxybenzoic acid dehydrogenase, respectively, and the nahg gene of the nah plasmid nah7 , which codes for salicylate hydroxylase, were cloned in pbr322 vector plasmid. deletion and insertion mutagenesis were used to localize these genes with respect to crucial endonuclease cleavage sites. the pbr322-based plasmids were ligat ...	1984	6327621
isolation and expression of rhizobium japonicum cloned dna encoding an early soybean nodulation function.	a first visible step in the nodulation of legumes by rhizobium spp. is the deformation and curling of root hairs. we have identified and cloned dna sequences encoding this function from two strains of rhizobium japonicum (usda 122 and usda 110) with a weakly homologous probe from rhizobium meliloti. root hair curling encoded by the cloned dna fragments was examined on soybeans (glycine soja ) after conjugative transfer of these sequences in broad-host-range vectors to various bacterial genera. p ...	1984	6327649
transposon tn5 encodes streptomycin resistance in nonenteric bacteria.	strains of caulobacter crescentus, pseudomonas putida, acinetobacter calcoaceticus, rhizobium meliloti, and rhodopseudomonas sphaeroides carrying the kanamycin resistance-encoding transposon tn5 were 15 to 500 times more resistant to streptomycin than transposon-free strains. the streptomycin resistance determinant, which is separable from the kanamycin resistance determinant of tn5, was not expressed in escherichia coli or klebsiella aerogenes.	1984	6330041
the use of mudlac transposons as tools for vital staining to visualize clonal and non-clonal patterns of organization in bacterial growth on agar surfaces.	when a histochemical stain for beta-galactosidase activity is applied to growth of gram-negative bacteria on agar medium, the pigmentation is non-uniform and capable of revealing internal colony organization into different cell types. use of an escherichia coli strain with a thermosensitive lac repressor indicates that colonies expand by addition of new cells at the periphery and that older cells which have synthesized beta-galactosidase early in development remain in the centre. mixed inocula o ...	1984	6206197
escherichia coli and pseudomonas putida rna polymerases display identical contacts with promoters.	methylation protection experiments with four promoters (p1 and p2 of the pbr322 plasmid, lacuv5 and lambda p0) have shown that the rna polymerases from escherichia coli and pseudomonas putida, while differing in the primary structure of the subunits involved in dna binding, display identical patterns of dna contacts. nor do these enzymes differ in covalent cross-linking patterns with a partially apurinized promoter. we conclude that the two rna polymerases have very similar structures of dna bin ...	1984	6236350
resolution of 5-oxo-l-prolinase into a 5-oxo-l-proline-dependent atpase and a coupling protein.	5-oxo-l-prolinase catalyzes a reaction in which the endergonic cleavage of 5-oxo-l-proline to form l-glutamate is coupled to the exergonic cleavage of atp to adp and pi. in the present research, the enzyme present in a strain of pseudomonas putida isolated from soil by enrichment culture was found to be composed of two protein components. neither component alone could catalyze the 5-oxoprolinase reaction, but the reaction was effectively catalyzed when they were mixed. one component (a) exhibite ...	1984	6145710
purification and properties of amino acid racemase from aeromonas punctata subsp. caviae.	an amino acid racemase, which occurs in the cytoplasmic fraction of aeromonas punctata subsp. caviae, has been purified to homogeneity by the criteria of electrophoresis and ultracentrifugation. the enzyme has a molecular weight of about 80,000 and consists of two subunits identical in molecular weight (about 40,000). the enzyme contains 2 mol of pyridoxal 5'-phosphate per mol of enzyme, and exhibits absorption maxima at 280 nm and 420 nm. the holoenzyme is resolved by dialysis against hydroxyla ...	1984	6427786
3-chloro-d-alanine chloride-lyase (deaminating) of pseudomonas putida cr 1-1.		1984	6427787
effect of restricted aeration on catabolism of cholic acid by two pseudomonas species.	examination of some previously isolated bile acid-utilizing pseudomonas strains showed that pseudomonas sp. atcc 31752, together with other fluorescent strains, can be assigned to pseudomonas putida biotype b, whereas pseudomonas sp. atcc 31753, like most other nonfluorescent strains, is an unrecognized phenotype. a study was made of the growth of these two species at 25 degrees c and ph 7.0 in a fermentor with 2.5 g of sodium cholate liter-1 as sole carbon source, and the catabolism of the chol ...	1984	6476826
isolation and characterization of pseudomonas putida ppf1 mutants defective in the toluene dioxygenase enzyme system.	pseudomonas putida ppf1 degraded toluene via a dihydrodiol pathway to tricarboxylic acid cycle intermediates. the initial reaction was catalyzed by a multicomponent enzyme, toluene dioxygenase, which oxidized toluene to (+)-cis-1(s),2(r)-dihydroxy-3-methylcyclohexa-3,5-diene (cis-toluene dihydrodiol). the enzyme consisted of three protein components: nadh-ferredoxintol oxidoreductase (reductasetol), ferredoxintol, and a terminal oxygenase which is an iron-sulfur protein (isptol). mutants blocked ...	1984	6501223
inactivation of the pseudomonas striata broad specificity amino acid racemase by d and l isomers of beta-substituted alanines: kinetics, stoichiometry, active site peptide, and mechanistic studies.	mechanism-based inactivators were used to probe the active site of the broad specificity amino acid racemase from pseudomonas striata. kinetic parameters for the inactivation of the racemase with both stereoisomers of beta-fluoroalanine, beta-chloroalanine, and o-acetylserine were determined. by use of 14c-labeled o-acetylserines, the stoichiometry of inactivator binding was found to be one inactivator bound per enzyme subunit. the plp-dependent enzyme contains one coenzyme per subunit, and afte ...	1984	6439237
[phosphate and glucose accumulation by pseudomonas cultures in relation to their arsenic resistance].	the effect of arsenite and arsenate on 14c-glucose and 32-p-phosphate transport was studied in the cells of pseudomonas aeruginosa 561 sensitive to arsenite and in the cells of pseudomonas putida 18 oxidizing arsenite and resistant to arsenic. transport and accumulation of phosphate and glucose were inhibited in the presence of arsenite in the cells of p. aeruginosa 561 whereas arsenate inhibited only phosphate accumulation. arsenite and arsenate had hardly any effect at the initial transport ra ...	1984	6439982
chemical modification of tyrosine residues at the active centre of cytochrome p-450 cam.	soluble cytochrome p-450 cam from pseudomonas putida (ec 1.14.14.1) was chemically modified with tetranitromethane. at least five out of totally nine tyrosine residues are accessible to nitration as shown by tryptic peptide mapping using hplc. modification in the presence of the inhibitor metyrapone and subsequent peptide mapping indicate the location of one tyrosine residue at the active centre of cytochrome p-450 cam.	1984	6532454
structural analysis of the cysteine-containing peptides from the major 3-methylcholanthrene-induced isozyme of cytochrome p-450 (p-450c) in rat liver microsomes.	cytochrome p-450c, the major 3-methylcholanthrene-inducible isozyme of cytochrome p-450 in rat liver microsomes, was subjected to proteolytic digestion after s-carboxymethylation of the protein, and the peptides were resolved by high-pressure liquid chromatography. since it is now recognized that cytochromes p-450 contain a thiolate as the axial fifth ligand of the heme, seven peptides containing eight cysteines were subjected to microsequence analysis. one cysteine-containing peptide (tsa-56) w ...	1984	6477879
aromatic acids are chemoattractants for pseudomonas putida.	a quantitative capillary assay was used to show that aromatic acids, compounds that are chemorepellents for escherichia coli and salmonella sp., are chemoattractants for pseudomonas putida prs2000. the most effective attractants were benzoate; p-hydroxybenzoate; the methylbenzoates; m-, p-, and o-toluate; salicylate; dl-mandelate; beta-phenylpyruvate; and benzoylformate. the chemotactic responses to these compounds were inducible. taxis to benzoate and m-toluate was induced by beta-ketoadipate, ...	1984	6501217
tol plasmid can prevent induction of chemotactic responses to aromatic acids.	growth conditions that elicited positive chemotaxis to benzoate and m-toluate in tol- pseudomonas putida cells failed to elicit taxis to these compounds in tol+ cells. the inability of tol+ cells to respond to these aromatic acids appears to be due to the preferential expression of tol-encoded genes for aromatic degradation over chromosomally encoded genes. expression of chromosomal genes for aromatic degradation is required for cells to form beta-ketoadipate, the inducer of benzoate and m-tolua ...	1984	6501222
use of ureidopenicillins for selection of plasmid vector transformants in pseudomonas aeruginosa and pseudomonas putida.	broad-host-range plasmids coding for beta-lactamase were successfully selected after transformation of pseudomonas strains. transformants of both pseudomonas aeruginosa and pseudomonas putida containing plasmid pro1614 were isolated in media containing low concentrations of piperacillin. these strains were also susceptible to other ureidopenicillins. similar selections of transformants with carbenicillin, ampicillin, or ticarcillin required high concentrations of antibiotics and yielded backgrou ...	1984	6321446
nucleotide sequence surrounding transcription initiation site of xylabc operon on tol plasmid of pseudomonas putida.	the xylabc operon on the tol plasmid directs the synthesis of enzymes for conversion of toluene to benzoate and is positively controlled by the regulatory gene xylr. in the study here the nucleotide sequence was determined for the regulatory region of this operon. the in vivo transcription initiation site of the operon was determined by s1 nuclease and reverse transcriptase mapping. rna was prepared from m-methylbenzyl alcohol-induced cells of pseudomonas putida and escherichia coli carrying ptn ...	1984	6324212
an investigation of the iron-sulphur proteins of benzene dioxygenase from pseudomonas putida by electron-spin-resonance spectroscopy.	benzene dioxygenase from pseudomonas putida comprises three components, namely a flavoprotein (nadh:ferredoxin oxidoreductase; mr 81000), an intermediate electron-transfer protein, or ferredoxin (mr 12000) with a [2fe-2s] cluster, and a terminal dioxygenase containing two [2fe-2s] iron-sulphur clusters (mr 215000), which requires two additional fe2+ atoms/molecule for oxygenase activity. the ferredoxin and the dioxygenase give e.s.r. signals in the reduced state with rhombic symmetry and average ...	1984	6324743
permeability of the boundary layers of bdellovibrio bacteriovorus 109j and its bdelloplasts to small hydrophilic molecules.	measurements of the sucrose-permeable and -impermeable volumes during bdellovibrio bacteriovorus attack on escherichia coli or pseudomonas putida showed that the volume of the bdelloplast increased over that of the substrate cell. although the pattern of the increase differed with the two organisms, the volumes reached maximum at about 60 min into the bdellovibrio growth cycle. by this time, the cytoplasmic membranes of the attacked cells were completely permeable to sucrose. the kinetics of inc ...	1984	6363383
relationship of lipoamide dehydrogenases from pseudomonas putida to other fad-linked dehydrogenases.	pseudomonas putida produces two lipoamide dehydrogenases, lpd-glc and lpd-val. lpd-val is specifically required as the lipoamide dehydrogenase of branched-chain keto acid dehydrogenase and lpd-glc fulfills all other requirements for lipoamide dehydrogenase. both proteins are dimers with one fad per subunit. lpd-glc has an absorption maximum at 455 nm, but lpd-val has a maximum at 460 nm. comparison of amino acid compositions revealed that lpd-glc was more closely related to escherichia coli and ...	1984	6373365
l-arginine utilization by pseudomonas species.	the utilization of arginine was studied in several different pseudomonas species. the arginine decarboxylase and agmatine deiminase pathways were found to be characteristic of pseudomonas species of group i as defined by palleroni et al. (1974). pseudomonas putida strains had three distinct arginine catabolic pathways initiated by arginine decarboxylase, arginine deiminase and arginine oxidase, respectively. the two former routes were also present in p. fluorescens and p. mendocina and in p. aer ...	1984	6423769
plasmid-determined silver resistance in pseudomonas stutzeri isolated from a silver mine.	a silver-resistant strain of pseudomonas stutzeri was isolated from a silver mine. it harbored three plasmids, the largest of which (pkk1; molecular weight, 49.4 x 10(6)) specified silver resistance. plasmid pkk1 was apparently nonconjugative but could be transferred to pseudomonas putida by mobilization with plasmid r68.45.	1984	6715284
purification of bacterial l-methionine gamma-lyase.	a rapid procedure for the purification of l-methionine gamma-lyase from pseudomonas putida icr 3460 by deae- toyopearl 650m and deae-sephadex a-50 column chromatography is presented. the enzyme was purified with an average yield of 75% and showed about 10-fold higher specific activity than the enzyme from p. putida (= p. ovalis) ifo 3738 reported previously (h. tanaka, n. esaki , and k. soda (1976) febs lett. 66, 307-311). the present enzyme has a molecular weight of about 172,000 and consists o ...	1984	6742420
[effect of plasmids from various incompatibility groups on the development of bacteriophages specific to pseudomonas aeruginosa and pseudomonas putida].	the aim of this work was to study the effect of plasmids belonging to different incompatibility groups on the growth of bacteriophages in pseudomonas aeruginosa and pseudomonas putida strains. the growth of bacteriophages was shown to be limited most often due to the presence in cells of plasmids belonging to the p-2 incompatibility group. plasmids of the inc p-2 group differed from one another in the spectrum of bacteriophages whose growth they limited. phages whose growth was suppressed in str ...	1984	6431239
novel reactivity of cytochrome p-450-cam. methyl hydroxylation of 5,5-difluorocamphor.	the interaction of the camphor hydroxylating p-450 isolated from pseudomonas putida grown on camphor (p-450-cam) with 5,5-difluorocamphor, a substrate analog in which the two methylene hydrogens at the normal site of hydroxylation have been replaced with fluorine, has been examined. this compound binds tightly to the enzyme with a dissociation constant and uv-visible absorption spectrum identical to that observed with d-camphor. in the presence of the reconstituted p-450-cam system, 5,5-difluoro ...	1984	6501299
metabolism of alpha-terpineol by pseudomonas incognita.	details of the metabolism of alpha-terpineol by pseudomonas incognita are presented. degradation of alpha-terpineol by this organism resulted in the formation of a number of acidic and neutral metabolites. among the acidic metabolites, beta-isopropyl pimelic acid, 1-hydroxy-4-isopropenyl-cyclohexane-1-carboxylic acid, 8-hydroxycumic acid, oleuropeic acid, cumic acid, and p-isopropenyl benzoic acid have been identified. neutral metabolites identified were limonene, p-cymene-8-ol, 2-hydroxycineole ...	1984	6525582
phenolic 9,10-secosteroids as products of the catabolism of bile acids by a pseudomonas sp.	the obligate aerobe, pseudomonas putida atcc 31752, efficiently utilises bile acids as a source of carbon and energy for growth and maintenance. when aeration is considerably restricted, a consequence to the catabolism of the bile acids in a fermentor is an accumulation of certain steroidal catabolites. evidence is presented to show that among these are hydroxy-9,10-seco-1,3,5 (10)-androstratriene-9, 17-diones and those from four of the common bile acids, cholic, chenodeoxycholic, hyodeoxycholic ...	1984	6537051
oxidation of glycine by pseudomonas putida requires a specific lipoamide dehydrogenase.	pseudomonas putida produces two lipoamide dehydrogenases with molecular weights of 49,000 and 56,000 designated lpd-val and lpd-glc, respectively. lpd-val is required for oxidation of valine, since it is specifically utilized as the e3 component of branched-chain keto acid dehydrogenase. since glycine oxidation by bacteria and mammals also requires lipoamide dehydrogenase, we desired to determine which lipoamide dehydrogenase would be used by the p. putida glycine oxidation system. when grown in ...	1984	6546487
reversal by dna amplifications of an unusual mutation blocking alkane and alcohol utilization in pseudomonas putida.	we analyzed the reversion of strains carrying alk208, a mutation in the alkbac (alkane utilization) region of the pseudomonas cam-oct plasmid. reversion of alk208 was stimulated 25 to 75-fold by small doses of uv-irradiation. all alkane hydroxylase-positive (alkb+) revertants proved to be aliphatic alcohol dehydrogenase-positive (alkc+) as well, whereas alkc+ revertants could be either alkb+ or alkb-. most of the alkb- alkc+ partial revertants produced alkc- segregants at measurable frequencies. ...	1984	6597334
[oral infection wtih pseudomonas putida].		1984	6599728
formaldehyde dehydrogenase from pseudomonas putida: a zinc metalloenzyme.	the nad+-dependent formaldehyde dehydrogenase from pseudomonas putida c-83 was found to contain 4 gram atoms of zinc per mol, corresponding to 2 gram atoms of zinc per subunit monomer. treatment of the enzyme with o-phenanthroline resulted in removal of 1 gram atom of zinc per subunit and caused a complete inactivation of the enzyme. the activity lost was restored by the addition of zinc ions, by which the zinc content was also reversed to almost the same level as that of the native enzyme. anot ...	1984	6526822
p-chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl coa: beta-ketoadipate coa transferase.	beta-ketoadipate enol-lactone hydrolase (ec 3.1.1.24) and succinyl coa: beta-ketoadipate transferase (ec 2.8.3.6) catalyze consecutive metabolic reactions in bacteria. the enzymes appear to be members of different families of related proteins. enzymes within the enol-lactone hydrolase family appear to have diverged so extensively that common ancestry sometimes is not directly evident from comparison of nh2-terminal amino acid sequences of the proteins. amino acid sequences at or near the active ...	1984	6591865
hydroxyproline 2-epimerase of pseudomonas. subunit structure and active site studies.	hydroxyproline 2-epimerase of pseudomonas putida was purified to homogeneity by an improved procedure. the native enzyme consists of two probably identical subunits. alkylation of the active site with labeled reagents resulted in the loss of 80-85% of the activity but the incorporation of only one alkyl group even though the active site contains a cys residue from each of the two subunits. this result suggests that the enzyme shows half-site reactivity. the labeled enzyme was further subjected t ...	1984	6706934
proton magnetic resonance studies of 7fe ferredoxins. three redox states of the [4fe-4s] cluster in a pseudomonas ovalis ferredoxin.	the oxidizability of a redox couple, [4fe-4s], in a 7fe ferredoxin extracted from pseudomonas ovalis was monitored by 1h-nmr. the iron-sulfur cluster in the ferredoxin was not only reducible (nagayama et al., 1983) but also oxidizable in its native form. this result provided the first verification of 3 redox states for a redox center in ferredoxin, 4fe, in the native form of the protein.	1984	6745423
degradation of 3-chlorobenzoate in soil by pseudomonads carrying biodegradative plasmids.	degradation of continuously added 3-chlorobenzoate (3-cb) was studied in samples of chernozem soil. soil columns were inoculated with pseudomonas putida growing on 3-cb and carrying the biodegradation plasmid and with pseudomonas aeruginosa incapable of growth on 3-cb and carrying the inserted biodegradation plasmid pbs 2 determining ortho-cleavage of the aromatic ring. while the 3-cb degradation was observed in both inoculated variants, the native microflora of the soil under study was incapabl ...	1984	6745818
coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome p-450d cdna from rat liver.	we determined the coding nucleotide sequence of the mrna for a 3-methylcholanthrene-inducible cytochrome p-450, p-450d, of rat liver by sequence analysis of cloned cdnas. the predicted amino acid sequence of the cytochrome is composed of 513 amino acids, and its nh2-terminal sequence of 30 amino acids completely coincides with that reported from analysis of the purified cytochrome p-450d. the amino acid composition of the deduced sequence also agrees well with that determined from the purified p ...	1984	6584898
camphor hydroxylase of pseudomonas putida: vestiges of sequence homology in cytochrome p-450cam, putidaredoxin, and related proteins.	the amino acid sequences of cytochrome p-450cam and putidaredoxin of the camphor hydroxylase [camphor, reduced-putida-ferredoxin:oxygen oxidoreductase (5-hydroxylating), ec 1.14.15.1] of pseudomonas putida are compared to each other and then to the sequences of bovine adrenodoxin and cytochrome b5. the comparisons reveal areas of homology indicating that these four proteins may share a common evolutionary origin. moreover, homologous segments can be recognized by proper alignment of the sequence ...	1984	6584899
structure and function of the trp3 gene of saccharomyces cerevisiae: analysis of transcription, promoter sequence, and sequence coding for a glutamine amidotransferase.	the structure and function of the trp3 gene of saccharomyces cerevisiae were analyzed. subcloning of an original 4.8 kb bamhi dna fragment, carrying the yeast trp3 gene, allowed for a localization of the gene on a 2.5 kb clai/bamhi fragment. transcription was found to proceed from the clai site towards the bamhi site. three major transcription start sites were determined at positions -92, -87, and -81 by s1-mapping. the synthesis of the trp3 gene is regulated by the general control, and was foun ...	1984	24177735
[improved procedure for isolation and purification of methionine gamma-lyase from pseudomonas putida].	an improved, simplified and relatively rapid procedure is developed for isolation and purification of a new antitumor enzyme--methionine gamma-lyase from pseudomonas putida. the method includes five steps instead of seven steps in previous procedure with a good yield of the enzyme. the purified enzyme was shown to be homogeneous by the criteria of disc gel electrophoresis. the highly homogeneous preparations of the enzyme exhibited the absorption maxima at 280 and 420 nm. the detailed studies on ...	1983	6623989
heme binding and substrate-protected cysteine residues in p-450cam.	reactions of the pseudomonas putida cytochrome p-450-substrate complex or enzyme alone with 14c-labeled iodoacetic acid have been investigated at ph 7.0. after subsequent conversion of all of the cysteine residues to s-beta-carboxymethylcysteinyl residues, tryptic peptides of the derivative were separated by either high performance liquid chromatography or two dimensional electrophoresis, and their amino acid compositions and partial sequences were determined. all but cysteine residue-134 reacte ...	1983	6639664
the microbial oxygenation of the benzylisoquinoline alkaloid laudanosine.	the microbial transformation of the benzylisoquinoline alkaloid laudanosine by a strain of pseudomonas putida gives a metabolite in which o-demethylation of 1 methoxyl group of ring c, and introduction of 1 ketonic oxygen at c9 and 1 phenolic oxygen at ring c have occurred. also, o-methylcoripalline is formed in this transformation.	1983	6641902
rapid reaction studies on the oxygenation reactions of catechol dioxygenase.	the reaction of oxygen with catechol 1,2-dioxygenase from pseudomonas arvilla atcc 23974 in complex with catechol, 4-methylcatechol, and 4-fluorocatechol has been studied using single turnover stopped flow spectrophotometry. two sequential enzyme intermediates have been resolved and their visible spectra characterized by computer-assisted methods. these intermediates are spectrally similar to those observed in a similar study with protocatechuate dioxygenase (bull, c., ballou, d. p., and otsuka, ...	1983	6643492
enzymes of the beta-ketoadipate pathway in pseudomonas putida: kinetic and magnetic resonance studies of the cis,cis-muconate cycloisomerase catalyzed reaction.	steady-state kinetic analysis of the divalent metal ion requiring cis,cis-muconate cycloisomerase catalyzed interconversion of cis,cis-muconate and (+)-muconolactone obeys michaelis-menten kinetics and the haldane relationship from ph 6.2 to 8.3. the ph vs. kcat/km profiles suggest free-enzyme apparent pka values of 6.2 and 7.4: the reciprocal behavior of the data with respect to the latter pka value is consistent with base-acid catalysis by the enzyme involving proton removal from the lactone a ...	1983	6652062
[degradation of 3-chlorobenzoic acid by a pseudomonas putida strain].	a pseudomonas putida strain 87 capable of assimilating 3-chlorobenzoic acid as a sole source of carbon and energy (3cba+) was isolated. treatment with mitomycin c eliminated the 3cba+ phenotype in 1% of cells in the population. the 3cba+ phenotype was transferred at a low frequency in the process of conjugation to other bacteria belonging to the genus pseudomonas. determinants localized on the plasmid are presumed to be responsible for the capability to assimilate 3-chlorobenzoic acid. a scheme ...	1983	6664313
synthesis of d-cysteine-related amino acids by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1.	using the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1, s-methyl-, s-ethyl-, s-n-propyl-, s-n-butyl-, s-phenyl-, s-benzyl-, s-(2,3-dihydroxypropyl)- and s-(2-hydroxyethyl)-d-cysteine were synthesized from 3-chloro-d-alanine and methyl-, ethyl-, n-propyl-, n-butyl-, phenyl-, benzyl-mercaptans, alpha-thioglycerol and 2-mercaptoethanol, respectively. the enzymatically synthesized d-cysteine-related amino acids were isolated from the large scale react ...	1983	6838588
camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by pseudomonas putida.	previously, pseudomonas putida was shown to degrade (+)-camphor, and cleavage of the first ring of the bicyclic structure involved two monooxygenases (a hydroxylase and a ring oxygen-inserting enzyme), a dehydrogenase, and spontaneous cleavage of an unstable oxygenation product (lactone). cleavage of the second ring was not demonstrated but was assumed also to occur by ring oxygen insertion, since the predicted oxygenation product was extracted from whole-cell incubation systems. our investigati ...	1983	6848481
synthesis of s-(carboxymethyl)-d-cysteine by 3-chloro-d-alanine chloride-lyase of pseudomonas putida cr 1-1.	s-(carboxymethyl)-d-cysteine, which is an important component of semisynthetic cephalosporin, mt-141, was enzymatically synthesized. s-(ethoxy-carbonyl-methyl)-d-cystein was synthesized from 3-chloro- d-alanine and ethyl thioglycolate by the beta-replacement reaction of 3-chloro-d-alanine chloride-lyase from pseudomonas putida cr 1-1 and subsequently hydrolyzed by alkali. the synthesized s-(carboxymethyl)-d-cysteine was isolated from a large scale reaction mixture and identified physicochemicall ...	1983	6679711