Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| levofloxacin and ciprofloxacin in vitro activities against 4,003 clinical bacterial isolates collected in 24 italian laboratories. | levofloxacin showed comparable in vitro susceptibility to ciprofloxacin among enterobacteriaceae, pseudomonas aeruginosa, enterococci, and staphylococcus aureus, while greater susceptibility was observed in stenotrophomonas maltophilia and staphylococcus epidermidis, mainly when oxacillin resistant. the susceptibility of streptococcus pneumoniae to levofloxacin reached 99%. | 2003 | 12543701 |
| extended spectrum beta-lactamase (esbl)-producing enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. | extended spectrum beta-lactamase (esbl)-producing organisms pose unique challenges to clinical microbiologists, clinicians, infection control professionals and antibacterial-discovery scientists. esbls are enzymes capable of hydrolysing penicillins, broad-spectrum cephalosporins and monobactams, and are generally derived from tem and shv-type enzymes. esbls are often located on plasmids that are transferable from strain to strain and between bacterial species. although the prevalence of esbls is ... | 2003 | 12558458 |
| the influence of the chemical composition of drinking water on cuprosolvency by biofilm bacteria. | this study investigated the influence of water chemistry on copper solvation (cuprosolvency) by pure culture biofilms of heterotrophic bacteria isolated from copper plumbing. | 2003 | 12588559 |
| identification of mixed bacterial dna contamination in broad-range pcr amplification of 16s rdna v1 and v3 variable regions by pyrosequencing of cloned amplicons. | using a sensitive and rapid method combining broad-range pcr amplification of bacterial 16s rdna fragments and pyrosequencing for detection, identification and typing, we have found contaminating bacterial dna in our reagents used for pcr. identified bacteria are the water-borne bacterial genera pseudomonas, stenotrophomonas, xanthomonas, ralstonia and bacillus. our results are in concordance with recent reports of contaminated industrial water systems. in light of this conclusion, we believe th ... | 2003 | 12594028 |
| diversity of tetracycline resistance genes in bacteria from chilean salmon farms. | twenty-five distinct tetracycline-resistant gram-negative bacteria recovered from four chilean fish farms with no history of recent antibiotic use were examined for the presence of tetracycline resistance (tet) genes. sixty percent of the isolates carried 1 of the 22 known tet genes examined. the distribution was as follows. the tet(a) gene was found in six isolates. the tet(b) gene was found in two isolates, including the first description in the genus brevundimonas: two isolates carried the te ... | 2003 | 12604516 |
| bacteraemia in adults due to glucose non-fermentative gram-negative bacilli other than p. aeruginosa. | glucose non-fermentative gram-negative bacilli other than p. aeruginosa (nf) are emerging pathogens. | 2003 | 12615987 |
| respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna. | our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ... | 2003 | 12620850 |
| safety and use of sputum induction in children with cystic fibrosis. | we assessed the safety and use of induced sputum (is) in children with cystic fibrosis (cf). forty-eight children (19 males) with cf, mean age 12.6 (range, 7.3-17.0) years and median forced expired volume in 1 sec (fev(1)) 48% (range, 14-77%) predicted were recruited. patients spontaneously expectorated sputum and then performed sputum induction by inhalation of nebulized 7% hypertonic saline. samples were sent for bacteriological culture, and for measurement of the following inflammatory mediat ... | 2003 | 12629630 |
| a survey of bacterial diversity in ticks, lice and fleas from australia. | we isolated bacteria from ticks, lice and fleas. partial small subunit rrna sequences were obtained for each isolate and the closest matches in the fasta database were determined. these bacteria were mostly gram-positive (firmicutes), although representatives from the proteobacteria (alpha, beta, gamma subdivisions) and cfb group were also isolated. most of the isolates we found were from genera that were present in most of the ectoparasites studied, but a few genera were restricted to one speci ... | 2003 | 12632173 |
| oral colonisation by aerobic and facultatively anaerobic gram-negative rods and yeast in tibetans living in lhasa. | sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among tibetans living in lhasa, tibet autonomous region. mean ages of the study groups of children (38% females) and adults (61% females) were 11.6+/-0.9 and 37.1+/-6.1 years, respectively. all had lived in tibet since birth. oral rinse samples were sel ... | 2003 | 12642230 |
| fluctuation of bacteria isolated from elm tissues during different seasons and from different plant organs. | in this work we isolated a culturable endophytic aerobic heterotrophic bacterial community from the stem and root tissues of elm trees (ulmus spp.) and analyzed its fluctuations. a total of 724 bacterial isolates were collected at different times (april, june, september and december) from two elm trees, one infected with elm yellows phytoplasmas, and one which was healthy-looking. the isolates were grouped into 82 haplotypes, identified by means of amplified ribosomal dna restriction analysis (a ... | 2003 | 12648725 |
| the efflux pump inhibitor phe-arg-beta-naphthylamide does not abolish the activity of the stenotrophomonas maltophilia smedef multidrug efflux pump. | 2003 | 12654744 | |
| role of the acetyltransferase aac(6')-iz modifying enzyme in aminoglycoside resistance in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an emerging nosocomial pathogen that displays high-level intrinsic resistance to multiple antibiotics including aminoglycosides. a gene [aac(6')-iz] encoding an aminoglycoside-modifying enzyme, aac(6')-iz acetyltransferase, was recently cloned and sequenced in s. maltophilia, but its importance with respect to aminoglycoside resistance in this organism was not determined. using a homologous gene replacement approach, mutants carrying unmarked chromosomal deletions ... | 2003 | 12654758 |
| liver abscess caused by stenotrophomonas maltophilia: report of a case. | we report the case of a melioidosis-like abscess of the liver caused by stenotrophomonas (xanthomonas) maltophilia infection in a chinese man living in hungary. although this appears to be the first documentation of a liver abscess of this origin in a nonimmunocompromised patient, our case report demonstrates that this common facultative pathogen can also cause liver abscess and sepsis. after repeated negative blood cultures, histological examinations of liver biopsies suggested the possibility ... | 2003 | 12658392 |
| negative bacterial polymerase chain reaction (pcr) findings in prostate tissue from patients with symptoms of chronic pelvic pain syndrome (cpps) and localized prostate cancer. | the etiology of chronic pelvic pain syndrome (cpps) remains obscure. although, bacterial etiology has frequently been suggested, evidence of both bacterial involvement in cpps and the presence of normal bacterial flora in the prostate remain uncertain. | 2003 | 12661035 |
| transformations of selenate and selenite by stenotrophomonas maltophilia isolated from a seleniferous agricultural drainage pond sediment. | a gram-negative bacterium, identified as stenotrophomonas maltophilia by fatty acid analysis and 16s rrna sequencing, was isolated from a seleniferous agricultural evaporation pond sediment collected in the tulare lake drainage district, california. in cultures exposed to the atmosphere, the organism reduces selenate (seo4(2-)) and selenite (seo3(2-)) to red amorphous elemental selenium (se degrees ) only upon reaching stationary phase, when o2 levels are less than 0.1 mg l(-1). in 48 h, s. malt ... | 2003 | 12662176 |
| an outbreak of lymphadenitis associated with stenotrophomonas (xanthomonas) maltophilia in omani goats. | stenotrophomonas maltophilia is a commonly isolated organism from human clinical specimens and is gaining significance as a pathogen in immunocompromised patients and nosocomial infections. in most cases it is difficult to establish the source of human infections. in veterinary medicine, s. maltophilia is not generally considered a primary pathogen. in the present study, we report the occurrence of 16 cases of caprine abscess from which s. maltophilia was isolated in pure culture from 15 animals ... | 2003 | 12675904 |
| microbial contamination of embryos and semen during long term banking in liquid nitrogen. | we report on microbial contamination of embryos and semen cryopreserved in sealed plastic straws and stored for 6-35 years in liquid nitrogen. there were 32 bacterial and 1 fungal species identified from randomly drawn liquid nitrogen, frozen semen, and embryos samples stored in 8 commercial and 8 research facility liquid nitrogen (ln) tanks. the identified bacteria represented commensal or environmental microorganisms and some, such as escherichia coli, were potential or opportunistic pathogens ... | 2003 | 12686204 |
| [indications and results of retroperitoneal laparostomy in the treatment of infected acute necrotizing pancreatitis]. | the aim of this study is to describe personal experience with retroperitoneal laparostomy in the management of infected acute necrotizing pancreatitis. the presence of an infected phlegmon requires surgical debridement and drainage. the surgical approach can be either an anterior laparotomy with irrigation and drainage (which can be either an open or closed laparotomy) or a posterior laparostomy. three patients (2 men and 1 woman) presented with an unfavourable course of their acute necrotizing ... | 2003 | 12692503 |
| in vitro activity of gatifloxacin alone and in combination with cefepime, meropenem, piperacillin and gentamicin against multidrug-resistant organisms. | to study the in vitro interaction of gatifloxacin in combination with gentamicin and with the beta-lactams cefepime, meropenem and piperacillin against clinical isolates of stenotrophomonas maltophilia, pseudomonas aeruginosa, burkholderia cepacia, extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae, vancomycin-resistant enterococcus faecium (vre) and methicillin-resistant staphylococcus aureus (mrsa). | 2003 | 12697632 |
| risk factors for stenotrophomonas maltophilia bacteremia in oncology patients: a case-control study. | to characterize risk factors for stenotrophomonas maltophilia bloodstream infection in oncology patients. | 2003 | 12725356 |
| comparative in vitro potency of amoxycillin-clavulanic acid and four oral agents against recent north american clinical isolates from a global surveillance study. | the in vitro activity of amoxycillin-clavulanic acid was compared with four comparator oral antimicrobial agents; ampicillin, azithromycin, cefuroxime and trimethoprim-sulphamethoxazole against 4536 recent clinical isolates covering 29 species isolated in the us and canada between 1997 and 1999. based upon minimum inhibitory concentrations (mics), amoxycillin-clavulanic acid was the most active agent against many gram-positive species and phenotypes including methicillin susceptible staphylococc ... | 2003 | 12727075 |
| effects of permeabilizers on antimicrobial susceptibility of stenotrophomonas maltophilia and acinetobacter spp. | isolates of the gram-negative bacteria stenotrophomonas maltophilia and acinetobacter spp. are naturally resistant to many classes of antibiotics. using the disk diffusion technique it was shown that the membrane permeabilizers ethylenediaminetetraacetic acid, sodium citrate, and sodium polyphosphate increased susceptibility to a range of antibiotics including imipenem, ciprofloxacin, tetracycline, and rifampicin. these effects are probably due to the metal chelating properties of the permeabili ... | 2003 | 12741739 |
| stenotrophomonas maltophilia septicemia with pyomyositis in a chemotherapy-treated patient. | pyomyositis is a rare complication of chemotherapy. a 35-year-old male patient with myelodysplastic syndrome developed stenotrophomonas maltophilia bacteremia shortly after chemotherapy, and stenotrophomonas maltophilia-related pyomyositis was encountered after recovery from neutropenia. he recovered completely after surgical drainage and a protracted course of antibiotic treatment. it is postulated that subclinical myopathy, immunosuppression secondary to the malignancy, or chemotherapeutic dru ... | 2003 | 12750843 |
| taxonomic positioning of two biological control agents for plant diseases as lysobacter enzymogenes based on phylogenetic analysis of 16s rdna, fatty acid composition and phenotypic characteristics. | to taxonomically position two bacterial strains conferring biological control activity towards plant diseases. | 2003 | 12752818 |
| role of phosphoglucomutase of stenotrophomonas maltophilia in lipopolysaccharide biosynthesis, virulence, and antibiotic resistance. | a homologue of the algc gene, responsible for the production of a phosphoglucomutase (pgm) associated with lps and alginate biosynthesis in pseudomonas aeruginosa, spgm, was cloned from stenotrophomonas maltophilia. the spgm gene was shown to encode a bifunctional enzyme with both pgm and phosphomannomutase activities. mutants lacking spgm produced less lps than the spgm(+) parent strain and had a tendency for shorter o polysaccharide chains. no changes in lps chemistry were obvious as a result ... | 2003 | 12761084 |
| infection control recommendations for patients with cystic fibrosis: microbiology, important pathogens, and infection control practices to prevent patient-to-patient transmission. | 2003 | 12762292 | |
| stenotrophomonas maltophilia infection related mortality during induction in childhood acute lymphoblastic leukemia. | 2003 | 12764762 | |
| stenotrophomonas (xanthomonas) maltophilia as an emerging opportunistic pathogen in association with hiv infection: a 10-year surveillance study. | stenotrophomonas (xanthomonas) maltophilia has been increasingly reported as a nosocomial opportunistic pathogen, responsible for serious infectious complications in immunocompromised patients. at present very limited information is available concerning its clinical significance in the setting of hiv infection. | 2003 | 12789473 |
| microbial consortia that degrade 2,4-dnt by interspecies metabolism: isolation and characterisation. | two consortia, isolated by selective enrichment from a soil sample of a nitroaromatic-contaminated site, degraded 2,4-dnt as their sole nitrogen source without accumulating one or more detectable intermediates. though originating from the same sample, the optimised consortia had no common members, indicating that selective enrichment resulted in different end points. consortium 1 and consortium 2 contained four and six bacterial species respectively, but both had two members that were able to co ... | 2003 | 12801097 |
| development of a gram-negative selective agar (gnsa) for the detection of gram-negative microflora in sputa in patients with cystic fibrosis. | to develop a selective agar medium to help detect and quantify gram-negative flora in the sputum of patients with cystic fibrosis (cf). | 2003 | 12807467 |
| occurrence and antimicrobial resistance of gram-negative bacteria isolated in haemodialysis water and dialysate of renal units: results of a greek multicentre study. | to evaluate the occurrence, identity and antimicrobial resistance of gram-negative bacteria isolated from municipal water supplies, treated water, and dialysate of all 85 greek haemodialysis centres. | 2003 | 12807469 |
| molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. | chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. the objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. washes and/or tissue samples from endoscopic sinus surgery on 11 patie ... | 2003 | 12808082 |
| [continuous surveillance of antimicrobial resistance among nosocomial gram-negative bacilli from intensive care units in china]. | to investigate the change of antimicrobial resistance among nosocomial gram-negative bacilli, especially those of enterobacteriaceae isolated from intensive care units from 1994 to 2001 in china. | 2003 | 12820912 |
| [changes of antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units from 1994 to 2001 in china]. | to investigate the antimicrobial resistance among nonfermenting gram-negative bacilli isolated from intensive care units (icus) during the past 7 years in china. | 2003 | 12820914 |
| differences in the permeability of high-flux dialyzer membranes for bacterial pyrogens. | the increasing use of high-flux membranes for hemodialysis has raised concerns that patients dialyzed with these membranes may be at higher risk of being exposed to cytokine-inducing bacterial substances in the dialysate than patients dialyzed with low-flux membranes. we investigated the permeability of various high-flux membranes for both purified e. coli lipopolysaccharide (lps) as well as for lps derived from stenotrophomonas (sten.) maltophilia. | 2003 | 12834177 |
| clinical role of beta-lactam/beta-lactamase inhibitor combinations. | the use of beta-lactamase inhibitors in combination with beta-lactam antibiotics is currently the most successful strategy to combat a specific resistance mechanism. their broad spectrum of activity originates from the ability of respective inhibitors to inactivate a wide range of beta-lactamases produced by gram-positive, gram-negative, anaerobic and even acid-fast pathogens. clinical experience confirms their effectiveness in the empirical treatment of respiratory, intra-abdominal, and skin an ... | 2003 | 12834367 |
| [endocarditis caused by stenotrophomonas maltophilia: report of a case and review of literature]. | we report a case of a 65 year-old woman with late endocarditis of prosthetic aortic valve and paravalvular abscess by stenotrophomonas maltophilia, which had an acute presentation for the memtionated abscess broken, with fever, bacteremia and congestive heart failure secondary to severity aortic regurgitation. itacute;s a rare cause of endocarditis with only 22 cases descripted in medical literature, the most of them in parenteral drug addict and as complication of cardiac valve replacement. the ... | 2003 | 12848604 |
| highly sensitive determination of ectoine and other compatible solutes by anion-exchange chromatography and pulsed amperometric detection. | in saline media prokaryotes compensate for the osmotic pressure of the surrounding medium by producing osmolytes. although these osmolytes or osmoprotectors have quite diverse structures, most of them can be determined by anion-exchange chromatography combined with integrated pulsed amperometric detection. this technique offers the advantages of very high sensitivity and new opportunities to determine ectoine and 5-hydroxyectoine-two important osmolytes -after hydrolytic cleavage of the pyrimidi ... | 2003 | 12851735 |
| the differential beta-lactamase activity of stenotrophomonas maltophilia. | 2003 | 12855245 | |
| antimicrobial activity and interference of tobramycin and chloramphenicol on bacterial adhesion to intraocular lenses. | the antimicrobial activities of tobramycin and chloramphenicol were evaluated by determining minimum inhibitory and bactericidal concentrations against staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, group a, group b and group g streptococci, klebsiella spp., stenotrophomonas maltophilia and ciprofloxacin-resistant and -susceptible pseudomonas aeruginosa, as well as by evaluating interference on adhesion of slime producer strains of s. aureus and p. aeruginosa to intrao ... | 2003 | 12866361 |
| aerobic biodegradation of nonylphenol by cold-adapted bacteria. | three strains capable of mineralizing nonylphenol as sole carbon source were isolated from a sample of contaminated soil and characterized as two pseudomonas spp. and a stenotrophomonas sp. the two pseudomonas spp. expressed characteristics typical of psychrophiles growing optimally of 10 degrees c and capable of growing at 0 degree c. the stenotrophomonas sp. was more likely psychrotrophic because it had an optimal temperature between 14 and 22 degrees c although it was not capable of growing a ... | 2003 | 12882175 |
| evaluation of the bd phoenix automated identification and susceptibility testing system in clinical microbiology laboratory practice. | the phoenix automated microbiology system (bd biosciences, usa) is a new, fully automated system for the rapid identification and antimicrobial susceptibility testing of gram-positive and gram-negative bacteria. the objective of this study was to evaluate the quality of performance of the phoenix system in the identification and antimicrobial susceptibility testing of 260 gram-negative ( n=174) and gram-positive ( n=86) isolates collected from polish hospitals in recent years. two phoenix panel ... | 2003 | 12884060 |
| bacterial diversity in cases of lung infection in cystic fibrosis patients: 16s ribosomal dna (rdna) length heterogeneity pcr and 16s rdna terminal restriction fragment length polymorphism profiling. | the leading cause of morbidity and mortality in cystic fibrosis (cf) patients stems from repeated bacterial respiratory infections. many bacterial species have been cultured from cf specimens and so are associated with lung disease. despite this, much remains to be determined. in the present study, we characterized without prior cultivation the total bacterial community present in specimens taken from adult cf patients, extracting dna directly from 14 bronchoscopy or sputum samples. bacterial 16 ... | 2003 | 12904354 |
| novel nosocomial infections by stenotrophomonas maltophilia: first reported case from lucknow, north india. | we describe a case of empyema with infected ascites caused by stenotrophomonas maltophilia, which has rarely been reported as pathogenic. the source was determined to a disinfectant solution. the isolate was sensitive to a newer carbapenem-meropenem, and the patient was treated successfully. this case represents a novel dual presentation of a nosocomial infection by the isolate in question. | 2003 | 12904437 |
| efflux as a mechanism of resistance to antimicrobials in pseudomonas aeruginosa and related bacteria: unanswered questions. | pseudomonas aeruginosa is an opportunistic human pathogen exhibiting innate resistance to multiple antimicrobial agents. this intrinsic multidrug resistance is caused by synergy between a low-permeability outer membrane and expression of a number of broadly-specific multidrug efflux (mex) systems, including mexab-oprm and mexxy-oprm. in addition to this intrinsic resistance, these and three additional systems, mexcd-oprj, mexef-oprn and mexjk-oprm promote acquired multidrug resistance as a conse ... | 2003 | 12917802 |
| fimbriae and adherence of stenotrophomonas maltophilia to epithelial cells and to abiotic surfaces. | stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen associated with several infectious diseases and opportunistic infections, especially in immunocompromised patients. these bacteria adhere avidly to medical implants and catheters forming a biofilm that confers natural protection against host immune defences and different antimicrobial agents. the nature of the bacterial surface factors involved in biofilm formation on inert surfaces and in adherence of s. maltophilia to ep ... | 2003 | 12925132 |
| [three cases of stenotrophomonas maltophilia pneumonia]. | we encountered 3 cases of pneumonia caused by stenotrophomonas maltophilia between january and june 2001. s. maltophilia is resistant to broad-spectrum antibiotics including carbapenem. reported studies indicate that excessive use of broad-spectrum antibiotics may induce resistance in this organism. however, our data showed that there was no clear correlation between the amounts of carbapenems used in our hospital and the isolation of the organism. if broad-spectrum antibiotics are ineffective o ... | 2003 | 12931674 |
| neural stem cells: a potential source for remyelination in neuroinflammatory disease. | in multiple sclerosis, the central nervous system is lesioned through invasion of plaque-forming inflammatory cells, primarily contributing to immune attack of myelin and oligodendrocytes. in this report we address the possible activation and differentiation of central nervous system stem cells following such immunological insults in a well-characterized rat model of multiple sclerosis characterised by spinal cord pathology. dye-labeled central nervous system stem cells, residing within the epen ... | 2003 | 12946021 |
| [phenotypic and genotypic characteristics of non fermenting atypical strains recovered from cystic fibrosis patients]. | we used partial 16s rrna gene (16s dna) sequencing for the prospective identification of nonfermenting gram-negative bacilli recovered from patients attending our cystic fibrosis center (hôpital necker-enfants malades), which gave problematic results with conventional phenotypic tests. during 1999, we recovered 1093 isolates of nonfermenting gram-negative bacilli from 702 sputum sampled from 148 patients. forty-six of these isolates (27 patients) were not identified satisfactorily in routine lab ... | 2003 | 12948761 |
| pseudomonas aeruginosa and burkholderia cepacia cannot be detected by pcr in the breath condensate of patients with cystic fibrosis. | the collection of sputum for microbiological examination in young cystic fibrosis patients can be very difficult. however, a knowledge of bacterial flora colonizing the patient's airways is of paramount importance for proper antimicrobial therapy. it is also known that cystic fibrosis patients colonized by pseudomonas species have a poorer prognosis than pseudomonas-negative patients. noninvasive ways of diagnosing airway inflammation that require only minimal cooperation of the patient might yi ... | 2003 | 12950050 |
| partial characterization of chorionic gonadotropin-like binding sites from the bacteria xanthomonas maltophilia. | the gram-negative bacterium, xanthomonas maltophilia, has low- and high-affinity luteinizing hormone/chorionic gonadotropin (lh/cg)-binding sites, similar to the lh/cg receptor found in mammals. although the low-affinity site binds both lh and human cg (hcg), the high-affinity site is specific for hcg. in the current investigation, these two binding sites were independently isolated from x. maltophilia for further characterization. to isolate functional binding sites, we developed a solubilizati ... | 2003 | 12968064 |
| evidence for dose-dependent effects on plant growth by stenotrophomonas strains from different origins. | to assess the influence of stenotrophomonas on plants, the interaction of 16 stenotrophomonas strains from clinical and environmental sources with strawberry plant seedlings was analysed. | 2003 | 12969277 |
| the in-vitro antimicrobial effect of non-antibiotics and putative inhibitors of efflux pumps on pseudomonas aeruginosa and staphylococcus aureus. | the anti-microbial activity of six non-antibiotics (one amino-ethylchloride, three phenothiazines, two tricyclic antidepressives) were tested on 20 clinical isolates of pseudomonas aeruginosa, one clinical isolate of klebsiella pneumoniae, 2 attc strains and 14 clinical isolates of staphylococccus aureus, using the plate dilution method. the effects on p. aeruginosa were independent of antibiotic resistance pattern and the species stenotrophomonas maltophilia was found to be the most susceptible ... | 2003 | 13678831 |
| antimicrobial resistance among gram-negative organisms in the intensive care unit. | we review the hospital-acquired gram-negative organisms commonly encountered among patients in the intensive care unit and discuss pertinent surveillance data, resistance mechanisms and patterns, and optimal treatment regimens for these pathogens. | 2003 | 14508155 |
| characterisation of bacterial cultures enriched on the chlorophenoxyalkanoic acid herbicides 4-(2,4-dichlorophenoxy) butyric acid and 4-(4-chloro-2-methylphenoxy) butyric acid. | the aim of this study was to enrich and characterise bacterial consortia from soils around a herbicide production plant through their capability to degrade the herbicides 4-(2,4-dichlorophenoxy) butyric acid (2,4-db) and 4-(4-chloro-2-methylphenoxy) butyric acid (mcpb). partial 16s rrna gene sequencing revealed members of the genera stenotrophomonas, brevundimonas, pseudomonas, and ochrobactrum in the 2,4-db- and mcpb-degrading communities. the degradation of 2,4-db and mcpb was facilitated by t ... | 2003 | 14513383 |
| the susceptibility of non-fermentative gram-negative bacilli to cefperazone and sulbactam compared with other antibacterial agents. | this study was designed to determine the bacterial susceptibility of non-fermentative gram-negative organisms to various antibacterial agents. bacterial susceptibility testing used the kirby-bauer method and data were assessed according to nccls 2000. cefoperazone/sulbactam (cper/su) had good antibacterial activity against pseudomonas aeruginosa. its activity was next only to that of imipenem, meropenem and ceftazidime. cper/su was highly active against acinetobacter spp., alcaligenes spp., burk ... | 2003 | 14522108 |
| in vitro antimicrobial activity of moxifloxacin compared to other quinolones against recent clinical bacterial isolates from hospitalized and community-based cancer patients. | the in vitro spectrum of moxifloxacin (a c-8-methoxyquinolone) was compared to that of ciprofloxacin and levofloxacin against 924 recent clinical isolates from cancer patients. moxifloxacin was more active than the comparator agents against gram-positive pathogens, with potent activity against aerococcus spp., listeria monocytogenes, micrococcus spp., rhodococcus equi, and stomatococcus mucilaginous, methicillin-susceptible staphylococcus spp., all beta hemolytic streptococci, viridans streptoco ... | 2003 | 14522520 |
| stenotrophomonas maltophilia intestinal colonization in hospitalized oncology patients with diarrhea. | a 6-week surveillance study was performed to determine the prevalence of stenotrophomonas maltophilia intestinal colonization among patients hospitalized in an oncology unit who developed diarrhea. ninety-two stool samples obtained from 41 patients were cultured, and 4 patients (4 [9.5%] of 41 patients) had cultures that were positive for s. maltophilia. after controlling for duration of diarrhea, patients colonized with s. maltophilia had received a greater number of different types of antibiot ... | 2003 | 14523780 |
| acute tubular necrosis associated with propylene glycol from concomitant administration of intravenous lorazepam and trimethoprim-sulfamethoxazole. | a 46-year-old morbidly obese man was admitted to the medical intensive care unit with respiratory failure. he required pressure-control ventilation and high levels of sedation with continuous-infusion lorazepam. he developed stenotrophomonas maltophilia pneumonia; treatment included scheduled intravenous trimethoprim-sulfamethoxazole. each of these drugs contain several hundred milligrams/milliliter of propylene glycol. on day 17 of his hospital course, 3 days after starting the trimethoprim-sul ... | 2003 | 14524651 |
| pathophysiology and management of pulmonary infections in cystic fibrosis. | this comprehensive state of the art review summarizes the current published knowledge base regarding the pathophysiology and microbiology of pulmonary disease in cystic fibrosis (cf). the molecular basis of cf lung disease including the impact of defective cystic fibrosis transmembrane regulator (cftr) protein function on airway physiology, mucociliary clearance, and establishment of pseudomonas aeruginosa infection is described. an extensive review of the microbiology of cf lung disease with pa ... | 2003 | 14555458 |
| stenotrophomonas maltophilia: a serious and rare complication in patients suffering from burns. | stenotrophomonas maltophilia is rarely implicated in clinical infections but it constitutes a significant nosocomial pathogen, especially in immunocompromised patients. this report describes the first case of a generalised infection caused by s. maltophilia that included bacteremia, wound and respiratory tract infection in a patient suffering from burns. given the emergence of s. maltophilia nosocomial infections, especially in patients with burns, isolation of the bacterium from blood cultures ... | 2003 | 14556730 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2001--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2001 were yearly evaluated and compared with those of other cephems, oxacephems and carbapenems. a total of 3,245 strains in 32 species of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis, escherichia coli, citrobacter freundii, citrobact ... | 2003 | 14567255 |
| stenotrophomonas maltophilia contamination of nebulizers used to deliver aerosolized therapy to inpatients with cystic fibrosis. | there is circumstantial evidence that nebulizer equipment may be a source of stenotrophomonas maltophilia for patients with cystic fibrosis. eighty-nine inpatient nebulizers were examined for evidence of s. maltophilia contamination of which nine (10%) yielded 14 strains of the bacterium. environmental samples were obtained from 73 different sites on the ward, of which 17 (23%) yielded a further 21 strains. positive sites included taps, sink drains, and potable water. genotyping using eric-pcr a ... | 2003 | 14572484 |
| stenotrophomonas maltophilia infections. | stenotrophomonas maltophilia is a ubiquitous, gram-negative organism that causes hospital-acquired infections. persons often come in contact with s. maltophilia through environmental water sources, including hospital tap water or faucets, and it has been associated with nosocomial outbreaks of infection. s. maltophilia often infects debilitated persons and those with underlying medical conditions, including immunosuppression. manifestations of infection include pneumonia, often in mechanically v ... | 2003 | 16088528 |
| burkholderia and emerging pathogens in cystic fibrosis. | persons with cystic fibrosis (cf) are susceptible to respiratory tract infection with a variety of bacterial species. among these are several species in the genus burkholderia (including members of the b. cepacia complex, b. gladioli, and b. pseudomallei), stenotrophomonas maltophilia, achromobacter ( alcaligenes) xylosoxidans, certain ralstonia species, and species within the new genus pandoraea. enterobacteriaceae and several other rather unusual or novel species also can be found. many of the ... | 2003 | 16088584 |
| patient cohorting and infection control. | patients with cystic fibrosis (cf) have an abnormal propensity for recurrent and chronic infections of the lower respiratory tract (lrt), and the most common cause of a shortened lifespan is chronic infection with pseudomonas aeruginosa. a few other gram-negative organisms, primarily burkholderia cepacia complex have, however, emerged as serious pathogens capable of establishing chronic lrt infection. details of these and other cf pathogens can be found in the article by dr. john lipuma, burkhol ... | 2003 | 16088586 |
| production of n-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria. | quorum-sensing control mediated by n-acylhomoserine lactone (ahl) signal molecules has been established as a key feature in the regulation of various metabolic traits in many bacteria. approximately 300 strains representing 6 genera and 18 species of soil-borne and plant-associated gram-negative bacteria isolated in various regions of the former ussr using two reporter systems were screened for ahl production. the production was observed in 17.5% of the screened bacterial strains. positive respo ... | 2003 | 15058194 |
| involvement of a quinoprotein (pqq-containing) alcohol dehydrogenase in the degradation of polypropylene glycols by the bacterium stenotrophomonas maltophilia. | previous work has shown that when the bacterium stenotrophomonas maltophilia is grown on polypropylene glycol, different dye-linked polypropylene glycol dehydrogenase (ppg-dh) activities are induced during growth. here the purification and characterization of the dehydrogenase activity induced in the stationary phase, and present in the periplasmic space, is described. the homogeneous enzyme preparation obtained consists of a homodimeric protein with a molecular mass of about 123 kda and an isoe ... | 2003 | 12586415 |
| tylenchulus semipenetrans alters the microbial community in the citrus rhizosphere. | infection of citrus seedlings by tylenchulus semipenetrans was shown to reduce subsequent infection of roots by phytophthora nicotianae and to increase plant growth compared to plants infected by only the fungus. hypothetical mechanisms by which the nematode suppresses fungal development include nutrient competition, direct antibiosis, or alteration of the microbial community in the rhizosphere to favor microorganisms antagonistic to p. nicotianae. a test of the last hypothesis was conducted via ... | 2003 | 19265991 |
| stenotrophomonas maltophilia endocarditis. | stenotrophomonas maltophilia is a gram-negative bacillus that is increasingly associated with serious nosocomial infections, especially in immunocompromised patients; however, the occurrence of endocarditis due to this organism is rare. this report describes a case of s. maltophilia endocarditis associated with a central venous catheter. the literature on stenotrophomonas endocarditis is reviewed. given the high morbidity and mortality of these infections, early antibiotic therapy utilizing trim ... | 2002 | 12587628 |
| characterization of a new keratin-degrading bacterium isolated from deer fur. | a keratin-degrading bacterium was isolated from soil containing deer fur. an axenic culture of the keratin-degrading bacterium was obtained in liquid culture using a keratin enrichment technique. the isolated bacterium was gram negative and catalase- and oxidase-positive. transmission electron microscopic observations showed that the bacterium was rod-shaped, 1.0-1.3 microm long and 0.7 microm in diameter. phylogenetic analysis of 16s rdna revealed that the new isolate has only 90.6% homology wi ... | 2002 | 16233255 |
| meningitis caused by stenotrophomonas maltophilia: case report and review of the literature. | 2002 | 17159399 | |
| [effects induced by the introduction of highly active antiretroviral therapy (haart) on disseminated bacterial infection during hiv disease]. | in order to assess the frequency, risk factors, etiology, and clinical outcome of hiv-related sepsis and bacteremia, according to the introduction of highly active antiretroviral therapy (haart), all episodes occurred in patients hospitalized during years 1993-1995 (pre-haart era), have been compared with those diagnosed after haart introduction (years 1997-2000). on the whole, 498 patients suffered from sepsis/bacteremia in the two considered time periods, but the frequency of this complication ... | 2002 | 12702888 |
| fatty acid analysis of stenotrophomonas maltophilia clinical strains showing different susceptibility to antibiotics at 30 and 37 degrees c. | isolates of stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (tds) to antibiotics. both 30tds strains (at least 4 times lower value of minimum inhibitory concentration (mic) of an antibiotic at 30 than at 37 degrees c) and 37tds strains (at least 4 times lower value of mic at 37 than at 30 degrees c) were described. changes in the distribution of saturated and unsaturated fatty acids (fa) at 30 and 37 degrees c were considered as one of possible cau ... | 2002 | 12630330 |
| [second multicenter study of the in vitro susceptibility to piperacillin-tazobactam]. | a study was conducted in 1999 on the activity of piperacillin-tazobactam on 1349 strains collected in a spanish multicenter study involving 10 hospitals. a total of 897 gram-negative bacteria of the following genuses were collected: escherichia coli (169), klebsiella spp. (112), pseudomonas spp. (122), enterobacter spp. (92), serratia spp. (44), citrobacter spp. (37), proteus spp. (114), morganella spp. (49), acinetobacter spp. (47), stenotrophomonas spp. (47), haemophilus spp. (45), moraxella s ... | 2002 | 12582446 |
| nosocomial stenotrophomonas maltophilia strain with a broad host range ability to transfer antibiotic resistance. | 2002 | 12583560 | |
| successful treatment of multi-resistant stenotrophomonas maltophilia meningitis with ciprofloxacin in a pre-term infant. | 2002 | 12536991 | |
| characterization of a chitinase gene from stenotrophomonas maltophilia strain 34s1 and its involvement in biological control. | a chitinase gene was cloned on a 2.8-kb dna fragment from stenotrophomonas maltophilia strain 34s1 by heterologous expression in burkholderia cepacia. sequence analysis of this fragment identified an open reading frame encoding a deduced protein of 700 amino acids. removal of the signal peptide sequence resulted in a predicted protein that was 68 kda in size. analysis of the sequence indicated that the chitinase contained a catalytic domain belonging to family 18 of glycosyl hydrolases. three pu ... | 2002 | 11872449 |
| microscale biosensor for measurement of volatile fatty acids in anoxic environments. | a microscale biosensor for acetate, propionate, isobutyrate, and lactate is described. the sensor is based on the bacterial respiration of low-molecular-weight, negatively charged species with a concomitant reduction of no(-)(3) to n(2)o. a culture of denitrifying bacteria deficient in n(2)o reductase was immobilized in front of the tip of an electrochemical n(2)o microsensor. the bacteria were separated from the outside environment by an ion-permeable membrane and supplied with nutrients (excep ... | 2002 | 11872469 |
| stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. | a polyphasic taxonomic study was performed on 16 stenotrophomonas strains from environmental and clinical sources. a group of three plant-associated isolates were shown to be phenotypically different from the other strains. this group formed a separate physiological cluster (b1) with 42% heterogeneity to the other isolates. the defining characteristics of the new species were as follows: growth at 4 degrees c and the absence of growth at 40 degrees c; the utilization of xylose as a carbon source ... | 2002 | 12508851 |
| [microbiology and cystic fibrosis]. | the respiratory infections are the rule in sick persons of cystic fibrosis. after the colonization in one's early childhood by staphylococcus aureus and haemophilus influenzae, it is established the colonization produced by the mucous strains of pseudomonas aeruginosa, the principal etiogenic of this illness, that can become to isolate in more than 95% of sick persons at three years old. that's why the etiogenic factors of this bacterium are studied, as they are toxins, pigments, enzymes, and pr ... | 2002 | 12518659 |
| mutations in gyra and parc qrdrs are not relevant for quinolone resistance in epidemiological unrelated stenotrophomonas maltophilia clinical isolates. | clinical strains of stenotrophomonas maltophilia are often highly resistant to multiple antibiotics and this resistance is steadily rising. quinolones are included in the group of antimicrobial agents to which this microorganism is developing resistance. therefore, the aim of this study was to analyze the epidemiological relationship among 22 clinical isolates of s. maltophilia as well as the molecular mechanisms responsible for the acquisition of quinolone-resistance in these strains. the resul ... | 2002 | 12523620 |
| functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements. | at present there are three protein families that share a common structural domain, the alphabeta/betaalpha fold of class b beta-lactamases: zinc beta-lactamases, glyoxalases ii, and a-type flavoproteins. a detailed inspection of their superimposed structures was undertaken and showed that although these proteins contain binuclear metal sites in spatially equivalent positions, there are some subtle differences within the first ligand sphere that determine a distinct composition of metals. althoug ... | 2002 | 11847294 |
| topoisomerase ii and iv quinolone resistance-determining regions in stenotrophomonas maltophilia clinical isolates with different levels of quinolone susceptibility. | the quinolone resistance-determining regions (qrdrs) of topoisomerase ii and iv genes from stenotrophomonas maltophilia atcc 13637 were sequenced and compared with the corresponding regions of 32 unrelated s. maltophilia clinical strains for which ciprofloxacin mics ranged from 0.1 to 64 microg/ml. gyra (leu-55 to gln-155, escherichia coli numbering), gyrb (met-391 to phe-513), parc (ile-34 to arg-124), and pare (leu-396 to leu-567) fragments from strain atcc 13637 showed high degrees of identit ... | 2002 | 11850246 |
| in vitro activities of tigecycline (gar-936) against recently isolated clinical bacteria in spain. | the antimicrobial activities of tigecycline (gar-936) were compared with those of other agents against 1,087 strains recently isolated in 12 spanish medical centers. tigecycline showed activity against a wide spectrum of aerobic and anaerobic bacteria, including strains such as methicillin-resistant staphylococcus aureus, coagulase-negative staphylococci, penicillin-resistant streptococcus pneumoniae, enterococcus faecium, acinetobacter baumannii, and stenotrophomonas maltophilia. | 2002 | 11850282 |
| in vitro activity of clinafloxacin in comparison with other quinolones against stenotrophomonas maltophilia clinical isolates in the presence and absence of reserpine. | a total of 33 stenotrophomonas maltophilia clinical isolates were tested for their susceptibility to clinafloxacin in comparison with ciprofloxacin, levofloxacin, moxifloxacin, nalidixic acid, norfloxacin, sparfloxacin and trovafloxacin. the mic(50) and mic(90) were as follows: ciprofloxacin 4 and 64 microg/ml; clinafoxacin 0.5 and 4 microg/ml; levofloxacin 2 and 32 microg/ml; moxifloxacin 1 and 8 microg/ml; nalidixic acid 8 and 128 microg/ml; norfloxacin 64 and 256 microg/ml; sparfloxacin 1 and ... | 2002 | 11858908 |
| stenotrophomonas maltophilia endocarditis: a systematic review. | the disease characteristics, management, and outcome of stenotrophomonas maltophilia endocarditis were evaluated by examining the reports on the subject identified through a comprehensive literature search. twenty-three (17 male) cases of s.. maltophilia endocarditis were identified. mean age was 41 +/- 15 years. all patients presented with fever. prosthetic valves were involved in 12 (52%) cases. among native valves, the aortic valve was most frequently involved (50%), followed by the tricuspid ... | 2002 | 11863309 |
| 7alpha-oh epimerisation of bile acids via oxido-reduction with xanthomonas maltophilia. | the microbial 7alpha-oh epimerisation of cholic, chenodeoxycholic, and 12-ketochenodeoxycholic acids (7alpha-oh bile acids) with xanthomonas maltophilia cbs 827.97 to corresponding 7beta-oh derivatives with scarcity of oxygen is described. with normal pressure of oxygen the 7-oh oxidation products are obtained. no biotransformations are achieved in anaerobic conditions. the microbial 7alpha-oh epimerisation is achieved by oxidation of 7-oh function and subsequent reduction. partial purification, ... | 2002 | 11728521 |
| absolute configuration of 3-hydroxy acids formed by stenotrophomonas maltophilia: application of multidimensional gas chromatography and circular dichroism spectroscopy. | the soil bacterium stenotrophomonas maltophilia was found to transform various long-chain fatty acids selectively into 3-hydroxy fatty acids of shorter chain length. their chiral evaluation was performed by multidimensional gas chromatography (mdgc) on modified cyclodextrin phase comparing the enantiodistribution of 1,3-diol formed without loss of stereochemical information from a representative microbial product with those of synthetic (3rs)- and (3s)-1,3-diols. enantiomeric excesses of 84-98% ... | 2002 | 11748801 |
| comparison of two culture methods for detection of tobramycin-resistant gram-negative organisms in the sputum of patients with cystic fibrosis. | a culture method utilizing quantitative plating on antibiotic-containing media has been proposed as a technique for the detection of tobramycin-resistant organisms that is more sensitive than standard methods. typical sputum culture methods quantitate the relative amounts of each distinct morphotype, followed by antibiotic susceptibility testing of a single colony of each morphotype. sputum specimens from 240 cystic fibrosis patients were homogenized, serially diluted, and processed in parallel ... | 2002 | 11773088 |
| smec, an outer membrane multidrug efflux protein of stenotrophomonas maltophilia. | a homologue of the mexab-oprm multidrug efflux operon of pseudomonas aeruginosa, smeabc, was cloned from stenotrophomonas maltophilia by using, as a probe, a pcr product amplified from this organism with primers based on the mexb sequence. the smeabc genes were hyperexpressed in a mutant strain displaying resistance to several antimicrobials, including aminoglycosides, beta-lactams, and fluoroquinolones. deletions in smec but not smeb compromised this resistance, suggesting that smec contributed ... | 2002 | 11796339 |
| the major extracellular protease of the nosocomial pathogen stenotrophomonas maltophilia: characterization of the protein and molecular cloning of the gene. | stenotrophomonas maltophilia is increasingly emerging as a multiresistant pathogen in the hospital environment. in immunosuppressed patients, these bacteria may cause severe infections associated with tissue lesions such as pulmonary hemorrhage. this suggests proteolysis as a possible pathogenic mechanism in these infections. this study describes a protease with broad specificity secreted by s. maltophilia. the gene, termed stmpr1, codes for a 63-kda precursor that is processed to the mature pro ... | 2002 | 11796713 |
| susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network. | from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ... | 2002 | 11814764 |
| differential regulation of l1 and l2 beta-lactamase expression in stenotrophomonas maltophilia. | it has been reported that stenotrophomonas maltophilia l1 and l2 beta-lactamase expression is coordinated. we have isolated s. maltophilia mutants where (i) l1 is constitutively hyper-expressed and l2 is inducible; (ii) l2 is hyper-expressed and l1 is inducible; and (iii) l1 and l2 are constitutively hyper-expressed. the frequency of isolating type 1 and 2 mutants is c. 10(-7), indicating that promoter mutations are probably not involved and providing strong evidence that l1 and l2 expression is ... | 2002 | 11815585 |
| severe cellulitis/myositis caused by stenotrophomonas maltophilia. | to present a case of cellulitis/myositis due to stenotrophomonas maltophilia in the absence of trauma and to discuss a potentially novel treatment option. | 2002 | 11816260 |
| l-glucitol catabolism in stenotrophomonas maltophilia ac. | the carbohydrate catabolism of the bacterium stenotrophomonas maltophilia ac (previously named pseudomonas sp. strain ac), which is known to convert the unnatural polyol l-glucitol to d-sorbose during growth on the former as the sole source of carbon and energy, was studied in detail. all enzymes operating in a pathway that channels l-glucitol via d-sorbose into compounds of the intermediary metabolism were demonstrated, and for some prominent reactions the products of conversion were identified ... | 2002 | 11823194 |
| 16s ribosomal dna-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by pcr and denaturing gradient gel electrophoresis. | the bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (dgge). 16s ribosomal dna fragments, including v6, -7, and -8 regions, were amplified with universal primers and analyzed by dgge. the bacterial diversity in purified water determined by pcr-dgge banding patterns was significantly lower than that of other aquatic environments. the bacte ... | 2002 | 11823209 |
| multiple mechanisms of antimicrobial resistance in pseudomonas aeruginosa: our worst nightmare? | pseudomonas aeruginosa carries multiresistance plasmids less often than does klebsiella pneumoniae, develops mutational resistance to cephalosporins less readily than enterobacter species, and has less inherent resistance than stenotrophomonas maltophilia. what nevertheless makes p. aeruginosa uniquely problematic is a combination of the following: the species' inherent resistance to many drug classes; its ability to acquire resistance, via mutations, to all relevant treatments; its high and inc ... | 2002 | 11823954 |
| crystallization and preliminary x-ray data of the recombinant peptide amidase from stenotrophomonas maltophilia. | the peptide amidase from stenotrophomonas maltophilia selectively hydrolyses the c-terminal amide bond in peptide amides. crystals have been obtained by sitting-drop vapour diffusion from solution containing polyethylene glycol (peg) 6000, hepes ph 7.5, glycerine and sodium azide (nan(3)). the crystals belong to the monoclinic space group p2(1), with unit-cell parameters a = 74.18, b = 62.60, c = 101.91 a, beta = 90 degrees. x-ray data from these crystals diffracted at the european synchrotron r ... | 2002 | 11807268 |