Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [possible pathogen: porcine circovirus (pcv)]. | 1999 | 10486869 | |
| ultrastructure of porcine circovirus in persistently infected pk-15 cells. | the ultrastructure of porcine circovirus was examined in persistently infected porcine kidney (pk)-15 cells. virus-infected pk-15 cells had large numbers of intracytoplasmic inclusions, and a few cells had intranuclear inclusions. intracytoplasmic inclusions were dispersed throughout the cytoplasm but were most numerous in the perinuclear cytoplasm. inclusion were of various sizes, round to oval, and electron dense and were of two general types. inclusions of the first type were small (0.1-0.5 m ... | 1999 | 10490204 |
| observations on pigeon circovirus infection in ontario. | subclinical pigeon circovirus infection was diagnosed in 1-day-old to 6-week-old birds from a loft with no history of clinical disease. pigeons from other lofts presented with various illnesses and were found at necropsy to be concurrently infected with pigeon circovirus. | 1999 | 10495910 |
| a universal polymerase chain reaction for the detection of psittacine beak and feather disease virus. | a universal pcr assay was designed that consistently detected psittacine beak and feather disease virus (bfdv) in psittacine birds affected with psittacine beak and feather disease (pbfd) from different geographic regions across australia. primers within open reading frame 1 (orf1) of the bfdv genome consistently amplified a 717 bp product from blood and/or feathers of 32 birds with pbfd lesions. the pcr did not amplify a product from the feathers or blood from 7 clinically normal psittacine bir ... | 1999 | 10501171 |
| electron microscopical observations of psittacine beak and feather disease in an umbrella cockatoo (cacatua alba). | psittacine beak and feather disease (pbfd) was diagnosed in an umbrella cockatoo (cacatua alba) with severe feather dystrophy and loss. electron microscopically, the intranuclear and intracytoplasmic inclusion bodies observed by light microscopy were composed of viral particles forming paracrystalline arrays, whorls, semicircles or concentric circles. recovered viral particles from the skin and feather follicle tracts were icosahedral and 15 to 20 nm in diameter. | 1999 | 10535515 |
| detection of porcine circovirus from lesions of a pig with wasting disease in japan. | a wasting disease characterized by progressive weight loss and dyspnea has been observed in weaning pigs on a farm in yamagata prefecture in 1998. histopathologic findings in an affected pig were bronchointerstitial pneumonia and intracytoplasmic clusters of basophilic inclusions in macrophages of lymph nodes, which were similar to those in pigs with postweaning multisystemic wasting syndrome (pmws) recently reported in north america and europe. porcine circovirus (pcv)-like particles were obser ... | 1999 | 10563289 |
| multiplex pcr for detection and typing of porcine circoviruses. | sets of oligonucleotide primers were designed according to the sequences of the open reading frames (orfs) orf1 and orf2 of the prototype nonpathogenic pk-15 strain of porcine circovirus (pcv) type 1 (pcv-1). by the pcr performed with the various primer sets, genomic dna or rna from other bacterial or viral pathogens of the respiratory tracts of pigs could not be amplified. a positive amplification reaction could be visualized with dna extracted from a viral suspension containing as few as 10 vi ... | 1999 | 10565907 |
| persistent infection with chicken anaemia virus and some effects of highly virulent infectious bursal disease virus infection on its persistency. | chicken anaemia virus (cav) infectivity and the effect of highly virulent infectious bursal disease virus (hv ibdv) infection on cav's infectivity were examined in chickens inoculated with cav or inoculated dually with cav and hv ibdv. five chickens inoculated dually with hv ibdv at 35 days old and then with cav at 40 days old exhibited no clinical signs of disease, but showed atrophic bursae of fabricius when necropsied 4 weeks later. upon examining the chickens at 7 days postinoculation (dpi) ... | 1999 | 10607503 |
| development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. | an immunohistochemical method for the detection of type 2 porcine circovirus (pcv2) in paraffin-embedded tissue was developed. rabbits were inoculated with purified pcv2 to obtain a polyclonal antiserum. antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which pcv2 genome had been demonstrated by in situ hybridization. in all cases (18/18), the density and distribution of positive cells detected by in situ ... | 1999 | 12968735 |
| myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. | porcine circovirus 2 (pcv2) is a recently identified agent that has been associated with postweaning multisystemic wasting syndrome (pmws) in swine populations. in this report, the potential spectrum of disease associated with pcv2 is expanded by evidence of vertical transmission and associated reproductive failure. pcv2 was isolated from a litter of aborted piglets from a farm experiencing late-term abortions and stillbirths. severe, diffuse myocarditis was present in 1 piglet associated with e ... | 1999 | 12968736 |
| isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. | postweaning multisystemic wasting syndrome (pmws), an apparently new disease, has been recognized in swine herds in western canada. young pigs with this disease have progressive weight loss, tachypnea, dyspnea, and jaundice, accompanied by interstitial pneumonia, lymphadenopathy, hepatitis, and nephritis. we examined more than 400 pigs from more than 70 herds in alberta, saskatchewan, and manitoba with cases of pmws. a small virus was isolated from a range of tissues from 8 of 8 affected pigs ex ... | 1998 | 9442952 |
| identification of a protein essential for replication of porcine circovirus. | the largest open reading frame of porcine circovirus (orf 4) encodes a protein of 312 amino acids. the predicted gene product of orf 4 shows similarities to rep proteins of other plant circoviruses and geminiviruses. three motifs have been identified that are characteristic for proteins involved in rolling circle replication and the consensus sequence for a putative dntp-binding box (gks) has been found. in this paper, experimental evidence is presented which indicates that orf 4 encodes the rep ... | 1998 | 9472624 |
| isolation of porcine circovirus-like viruses from pigs with a wasting disease in the usa and europe. | samples of lung, liver, kidney, pancreas, spleen, and lymph node from pigs with postweaning multisystemic wasting syndrome from california (usa) and samples of mesenteric lymph nodes from similarly diseased pigs from brittany (france) were examined by light microscopy, in situ hybridization (ish), and/or virus isolation. whole genomic probes for porcine circovirus (pcv) and chicken anemia virus (cav) were used for ish. tissue homogenate supernatants were inoculated onto pk/15 cells for virus iso ... | 1998 | 9526853 |
| nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. | this article describes the nucleotide sequence of a porcine circovirus (pcv) which possesses a high degree of association with postweaning multisystemic wasting syndrome (pmws), a newly described disease of young pigs. the dna sequence of this pmws-associated pcv (pmws pcv) has 68% homology with that of a previously published nonpathogenic strain of pcv. the strains appear to be closely related yet distinct from one another. | 1998 | 9573301 |
| novel porcine circoviruses from pigs with wasting disease syndromes. | 1998 | 9602519 | |
| porcine circovirus infection in northern ireland. | 1998 | 9612920 | |
| characterization of cis-acting elements affecting strength and phloem specificity of the coconut foliar decay virus promoter. | during replication in its host plant, coconut foliar decay virus (cfdv) remains restricted to the phloem tissue. previous in vivo studies on subgenomic cfdv dna had provided evidence for the phloem specificity of the cfdv promoter. here, new promoter constructs are described which are distinguished by the presence or absence of various cis-acting signals and which gave rise to a 16-fold higher reporter gene (beta-glucuronidase) activity (reaching 30% of the cauliflower mosaic virus 35s promoter) ... | 1998 | 9634093 |
| transcription analysis of porcine circovirus (pcv). | this study focuses on gene expression of porcine circovirus (pcv) in order to identify viral genes and their corresponding mrna transcripts. by northern blot analysis, the existence of three mrnas could be demonstrated. two mrnas are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. the (+)-strand encoded mrna transcript is 990 nucleotides (nt) long and corresponds to the open reading frame (orf) 1, as shown by s1 mapping. the start point of this transcript is located a ... | 1998 | 9654680 |
| circovirus-like viral associated disease in weaned pigs in indiana. | inclusion bodies with staining affinity and ultrastructural characteristics typical of circoviruses that stained positive for porcine circovirus (pcv)-like virus were demonstrated in association with granulomatous lesions in multiple tissues of three clinically ill 10- to 12-week-old pigs. a syndrome of poor growth and wasting in 5-15% of weaned pigs was an intermittent problem on a 450-sow one-site farrow-to-finish swine farm in indiana. routine diagnostic testing did not demonstrate a cause. g ... | 1998 | 9684976 |
| detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. | swine infectious agents, especially viruses, are potential public health risks associated with the use of pig organs for xenotransplantation in humans. therefore, there is a need for better characterization of swine viruses and for the development of diagnostic tests for their detection. we report here isolation of a novel strain of porcine circovirus (pcv) from pigs with postweaning multisystemic wasting syndrome (pmws). affected pigs exhibited severe interstitial pneumonia and lymphoid depleti ... | 1998 | 9705388 |
| characterization of novel circovirus dnas associated with wasting syndromes in pigs. | porcine circovirus (pcv) was initially recognized as a contaminant of continuous pig kidney cell lines and was not thought to be pathogenic. antibodies reactive to the cell culture isolate of pcv (pcv pk-15) are prevalent in the swine population worldwide. recently, pcv pk-15-like antigen and nucleic acid were demonstrated in lesions associated with wasting syndromes in pigs in north america and europe. monoclonal antibodies raised to circoviruses isolated from pigs with wasting syndromes highli ... | 1998 | 9747726 |
| beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. | circoviruses are a diverse group of animal and plant pathogens with undefined relationships to one another but for their non-geminate, non-enveloped capsids and circular, single-stranded dna genomes. the sequences of the beak and feather disease virus and porcine circovirus genomic dnas are presented and analyzed in the context of the other members of the family. sequence comparisons, inferred phylogenies, and geographic occurrence suggest that the ambisense circoviruses, particularly the beak a ... | 1998 | 9787657 |
| psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. | cloning and sequencing of the circular, single-stranded dna of one isolate of psittacine beak and feather disease virus (bfdv) demonstrate a genome composed of a circular molecule of 1993 nucleotide bases. an analysis of the assembled replicative form demonstrated seven open reading frames (orfs) (three in the virion strand and four in the complementary strand), potentially encoding seven viral proteins of >8.7 kda. high amino acid sequence similarity was demonstrated between a potential 33.3-kd ... | 1998 | 9791035 |
| porcine circovirus infection in the republic of ireland. | 1998 | 9836405 | |
| viruses and virus-like particles identified in ostrich gut contents. | samples of either gut content from ostriches showing symptoms of enteritis, or allantoic fluid of eggs inoculated with ostrich isolates, were examined for the presence of viral agents by direct negative-contrast electron microscopy. only a few virus types could be identified with certainty, namely a circovirus (1 sample), a coronavirus (1 sample), a member of either the toga- or bunyaviridae (1 sample), enterovirus (16 samples) and paramyxovirus (26 samples). a large number of samples contained ... | 1998 | 9850509 |
| piglet wasting disease. | 1997 | 9466392 | |
| sequence of porcine circovirus dna: affinities with plant circoviruses. | the complete nucleotide sequence (1759 nt) of the ssdna genome of porcine circovirus (pcv) was determined from a cloned dsdna replicative form isolated from pcv-infected cells. sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (cav) but, surprisingly, the highest protein similarity was obtained between the product of the largest predicted pcv orf (orf1; encoding a potential protein of 35.7 kda) and a putative protei ... | 1997 | 9010307 |
| two mrnas are transcribed from banana bunchy top virus dna-1. | we have mapped the mrna transcripts of banana bunchy top virus (bbtv) dna-1. northern hybridization and 3' race analysis identified two poly-adenylated rnas associated with bbtv dna-1. previously, one major orf in the virion sense of dna-1 had been identified, which encoded a putative replication protein (rep). an mrna was identified in bbtv infected bananas that was clearly transcribed from this rep orf. further, a second transcript was identified which mapped to an orf completely within the re ... | 1997 | 9010308 |
| mapping and characterization of the origin of dna replication of porcine circovirus. | the origin of dna replication of porcine circovirus (pcv) was mapped to a 111-bp fragment. on top of a hairpin, a nonanucleotide (tagtattac) homologous to nonanucleotides of other viruses was identified. mutation of this element abolishes replication. pcv may be related to a virus family characterized by single-stranded circular dna genomes, rolling-circle replication, and homology of their rep proteins. | 1997 | 9032401 |
| [circovirus infections in pigeons]. | the first detection of circovirus infection in pigeons in germany is described. the existing experiences with this still little known pigeon disease are summarized and similarities to circovirus infections in other species are shown. | 1997 | 9157630 |
| detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. | 1997 | 9187809 | |
| a feather disease in senegal doves (streptopelia senegalensis) morphologically similar to psittacine beak and feather disease. | the pathogenesis and epidemiology of a feather disease in wild senegal doves (streptopelia senegalensis) which is morphologically similar to psittacine beak and feather disease (pbfd) was investigated. although the lesions in doves resembled pbfd there was little evidence for the presence of psittacine circovirus (pscv). haemagglutination activity (ha) using type a galah (eolophus roseicapillus) erythro-cytes was not detected in feathers or livers of affected doves as would occur in pbfd. low co ... | 1997 | 18483948 |
| circovirus-like particles in the bursae of young racing pigeons. | 1996 | 8677611 | |
| effect of porcine circovirus infection on porcine alveolar macrophage function. | the effect of porcine circovirus (pcv) infection of porcine alveolar macrophage cultures on some of the functional properties of these cells are reported. pcv infection of alveolar macrophages did not effect their ability to phagocytose and kill complement-coated yeast cells or the expression of fc or complement receptors. a transient increase in major histocompatibility complex (mhc) class i expression in pcv-infected cells were observed 4 days after infection and a decrease in the number of ce ... | 1996 | 8677632 |
| circovirus in pigeons. | 1995 | 8746857 | |
| occurrence and role of an early antigen and evidence for transforming ability of porcine circovirus. | by means of indirect immunofluorescence assay (ifa) using natural swine immune serum and hyperimmune serum from rabbits infected with porcine circovirus (pcv), a pcv antigen was detected present prior to the onset of viral and cellular dna synthesis in nucleoli of cells of synchronized and growth stimulated infected ps cell cultures grown for more than 12 h in the presence of hydroxyurea. the number of cells containing specifically fluorescing nucleoli increased with increasing time of growth in ... | 1995 | 7503680 |
| presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle. | antibodies reacting with porcine circovirus (pcv) were found in sera of humans, mice, and cattle by means of an indirect immunofluorescence assay (ifa) and an elisa. in man, the highest seroprevalence (23.9% in ifa and 30.2% in elisa) was found among hospitalized patients with fever of partially unclear etiology. non-hospitalized "healthy" persons of the former german democratic republic showed a significantly higher number of positive sera (ifa = 20%) than blood donors from berlin-west (ifa = 8 ... | 1995 | 7544971 |
| poly(a) addition site mapping and polyadenylation signal analysis in a plant circovirus replication-related gene. | the transcripts of a genomic component of coconut foliar decay virus (cfdv), a plant circovirus with a single-stranded dna genome, were characterized by sequencing the 3' termini of the respective cdna clones. it was shown that transcription of the putative replication-related gene terminated at one major site (six bases downstream of the termination codon) in electroporated barley mesophyll protoplasts and that the resulting transcripts were polyadenylated. a deletion downstream of the aataaa s ... | 1995 | 7645233 |
| pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. | the results of virus and antigen distribution following experimental infection of colostrum deprived pigs with pig circovirus (pcv) by oral/nasal and intravenous routes are reported. pcv and antigen were detected using virus isolation and indirect immunofluorescence on cryostat sections respectively. pcv antigen was detected in tissues throughout the body but primarily in spleen thymus, and lung. no pcv antigen or virus was detected in tissue samples from the central nervous system. examination ... | 1995 | 7667906 |
| circovirus infection in european racing pigeons. | 1995 | 7762128 | |
| distribution of antibodies to porcine circovirus in swine populations of different breeding farms. | an enzyme linked immunosorbent assay (elisa) was developed for mass antibody screening to porcine circovirus (pcv) in pig herds of different age groups and of different husbandries. infection with pcv was found to be common in all swine herds tested, with only one exception, a herd at a small farm. statistically, the percentage of pcv negative sera decreased and titer levels increased with increasing age of the pigs. within individual age groups, differences were found to exist between different ... | 1995 | 7794114 |
| chicken anaemia virus infection: molecular basis of pathogenicity. | chicken anaemia virus (cav) is a small virus of a unique type with a particle diameter of 23 to 25 nm and a genome consisting of a circular single-stranded (minus-strand) dna. this dna multiplies in infected cells via a circular double-stranded replicative intermediate, which was recently cloned. dna analysis of cav strains isolated in different continents revealed only minor differences among the various isolates. apparently, all cav isolates belong to a single serotype. cav is not related to o ... | 1995 | 18645763 |
| particles resembling circovirus in the bursa of fabricius of pigeons. | histological examination of the bursae from 12 pigeons under 4 months old revealed basophilic globular inclusion bodies, 5 to 25 microns in diameter, in the cytoplasm and the nuclei of the various bursal follicular cells. electron microscopy of these inclusions revealed large electron-dense areas containing non-enveloped icosahedral viral particles, 14-19 nm in diameter, either loosely arranged or in paracrystalline array. similar basophilic globular inclusion bodies were seen in the spleen and ... | 1994 | 7832721 |
| computer search of transcription control sequences in small plant virus dna reveals a sequence highly homologous to the enhancer element of histone promoters. | the positions of nucleotide sequences which can act as binding sites for plant transcriptional trans-acting protein factors have been mapped in dna of plant circovirus--coconut foliar decay virus (cfdv). it was found that cfdv promoter region contains sequence motif homologous to the regulatory type i element of plant histone genes. we have also found the presence of the element i motifs in dnas of other small plant viruses. taking into account the mechanism of regulation of histone gene express ... | 1994 | 7841461 |
| production, preliminary characterisation and applications of monoclonal antibodies to porcine circovirus. | the preparation of monoclonal antibodies (mabs) to porcine circovirus is described. preliminary characterisation was carried out on nine mabs obtained from two fusions and included isotyping, virus neutralisation assays and indirect immunofluorescence staining patterns obtained following immunostaining of both a porcine circovirus (pcv)-persistently infected pig kidney (pk/15/w) and vero (vero-pcv) cell line. significant differences in the staining patterns were observed in both cell lines which ... | 1994 | 7856071 |
| evidence of circovirus infection in british pigs. | 1994 | 7941278 | |
| some biological and physico-chemical properties of porcine circovirus. | some important biological and physico-chemical characteristics of porcine circovirus are reported. these include a study on the host distribution in nature, levels of colostrum-derived antibodies in piglets from sero-positive sows and the susceptibility of a range of cell cultures to infection with this virus. the results of haemagglutination studies, resistance to ph 3, chloroform and heat are also reported as are comparative buoyant densities and sedimentation coefficients of porcine circoviru ... | 1994 | 7941842 |
| infection of leucocyte cell cultures derived from different species with pig circovirus. | cultures of leucocyte cells were prepared from pig bone marrow, peripheral blood, lung washings, thymus and lymph nodes. cell cultures were also prepared from peripheral blood from sheep, cattle and a human. immunofluorescent (if) staining of all these cultures, following inoculation with pig circovirus (pcv), detected virus replication in all the cell cultures derived from pigs and in the cell cultures derived from cattle. virus replication in pig leucocyte cell cultures was confirmed by demons ... | 1994 | 7975152 |
| a retrospective study of circovirus infection in pigeons: nine cases (1986-1993). | circovirus infections were diagnosed in 12 pigeons from the united states 4 pigeons from australia, and 1 pigeon from canada (1986-1993). circovirus was identified by electron microscopic examination of basophilic botryoid cytoplasmic inclusions that had a histologic appearance similar to that of psittacine beak and feather disease virus inclusions. inclusions were seen in splenic, bursal, gut-associated, and bronchus-associated lymphoid tissue macrophages and in bursal epithelial cells. inclusi ... | 1994 | 8068746 |
| vaccination and challenge studies with psittacine beak and feather disease virus. | psittacine beak and feather disease virus (pbfdv) was administered to adult galahs (eolophus roseicapillus) by mouth or by intramuscular injection. concentration of pbfdv antibodies in serum and excretion of pbfdv were monitored by haemagglutination inhibition (hi) and haemagglutination (ha) respectively. after oral administration, 17 of 18 galahs remained clinically normal and a small rise in antibody titre was detected in 3 of 18 birds. after intramuscular administration, antibody was detected ... | 1993 | 8117208 |
| circovirus-like infection in a pigeon. | 1993 | 8286462 | |
| comparison of three animal viruses with circular single-stranded dna genomes. | no common antigenic determinants and no dna sequence homologies were detected when three animal viruses, chicken anaemia agent (caa), porcine circovirus (pcv), and psittacine beak and feather disease virus (pbfdv), all of which possess circular single-stranded dna genomes, were compared. negative contrast electron microscopy showed that pcv and pbfdv particles were 30% smaller than caa particles and lacked the surface structure of caa. | 1991 | 2006901 |
| purification and biochemical characterization of chicken anaemia agent. | chicken anaemia agent (caa) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and cscl. the purification method was dependent on the use of an antigen-detecting elisa based on a caa-specific monoclonal antibody. virus particles banded at a density of 1.33 to 1.34 g/ml in cscl and measured 23.5 +/- 0.8 nm in diameter. purified preparations contained one major polypeptide (mr 50,000) and a single-stranded, circular dna ... | 1990 | 2109040 |
| porcine circovirus antigens in pk-15 cell line (atcc ccl-33) and evidence of antibodies to circovirus in canadian pigs. | permanent infection of a pk-15 (atcc ccl-33) cell line by the porcine circovirus was demonstrated. this virus appears to be common in the canadian swine population as indicated by the presence of antibodies detected by indirect immunofluorescence or immunoperoxidase tests in the serum of culled sows and sows in commercial herds. one source of the pk-15 cell line was found to be free of circovirus antigen. | 1989 | 2686830 |
| viral dna from cells infected with porcine circovirus. | it has been demonstrated earlier that the genome of the porcine circovirus (pcv) isolated from virions is a circular single-stranded dna (ssdna). this report describes the isolation and characterization of pcv-specific dna from infected cell cultures. two types of double-stranded dna (dsdna) were observed which behaved like supercoiled and relaxed circular molecules, respectively. both types of dsdna displayed infectivity in transfection experiments and are regarded as replicative forms (rf) of ... | 1988 | 2851903 |
| calf thymus dna polymerase delta: purification, biochemical and functional properties of the enzyme after its separation from dna polymerase alpha, a dna dependent atpase and proliferating cell nuclear antigen. | we have established a novel procedure to purify calf thymus dna polymerase delta from cytoplasmic extracts. the enzyme has typical properties of dna polymerase delta including a 3' - greater than 5' exonuclease activity and efficiently replicates natural occurring genomes such as primed single-stranded m13 dna and single-stranded porcine circovirus dna, this last one thanks to an associated or contaminating primase activity. a processivity of at least a thousand bases was evident and this in the ... | 1988 | 2899882 |
| replication of single-stranded porcine circovirus dna by dna polymerases alpha and delta. | porcine circovirus is the only mammalian dna virus so far known to contain a single-stranded circular genome (tischer et al. (1982) nature 295, 64-66). replication of its small viral dna (1.76 kb) appears to be dependent on cellular enzymes expressed during s-phase of the cell cycle (tischer et al. (1987) arch. virol. 96, 39-57). in this paper we have exploited the porcine circovirus genome to probe for in vitro initiation and elongation of dna replication by different preparations of calf thymu ... | 1988 | 3207761 |
| replication of porcine circovirus: induction by glucosamine and cell cycle dependence. | multiplication of porcine circovirus (pcv) was found to be inducible by treatment of infected cell cultures with 300 mm glucosamine. one day after glucosamine treatment and after growth in fresh medium, an increase in the number of cells containing virus antigen of up to 50 times as compared to mock-treated cultures was observed. analysis of this phenomenon revealed that replication of pcv dna was induced. only aminohexoses but not hexoses and acetylated aminohexoses were efficacious. the course ... | 1987 | 3619654 |
| mammalian dna polymerase alpha: a replication-competent holoenzyme form from calf thymus. | calf thymus dna polymerase alpha, like the replication-specific dna polymerase iii holoenzyme of escherichia coli, can be isolated as a distinct complex. a specific multiprotein form of the polymerase alpha, a form designated replication-competent (rc) holoenzyme, consists of a complex of a polymerase-primase core and at least six other polypeptides. the rc holoenzyme can efficiently replicate several naturally occurring templates, including the genomic dna of the porcine circovirus (pcv). the d ... | 1987 | 2894679 |
| studies on epidemiology and pathogenicity of porcine circovirus. | antibodies to porcine circovirus (pcv) which is the smallest animal virus known so far were found in 77-95 per cent of sera from slaughter pigs gathered in berlin and two districts of northern germany. about 60 per cent of these positive sera had relatively high titres similar to those in experimentally infected pigs 3-6 weeks after infection. this indicates that the animals might have become infected during the fattening period. sera from 2-3 year old pigs from a laboratory animal breeding inst ... | 1986 | 3778212 |