Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| type iii secretion homologs are present in brucella melitensis, b. ovis, and b. suis biovars 1, 2, and 3. | protein sequences from characterized type iii secretion (tts) systems were used as probes in silico to identify several tts gene homologs in the genome sequence of brucella suis biovar 1 strain 1330. four of the genes, named flhb, flip, flir, and flif on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in pcr and hybridization assays to determine their distribution among other brucella nomen species and biovars. the results indicated that flhb, flip, flir and ... | 2003 | 12732970 |
| isolation and expression of recombinant antibody fragments to the biological warfare pathogen brucella melitensis. | brucella melitensis is a highly infectious animal pathogen able to cause a recurring debilitating disease in humans and is therefore high on the list of biological warfare agents. immunoglobulin genes from mice immunized with gamma-irradiated b. melitensis strain 16m were used to construct a library that was screened by phage display against similarly prepared bacteria. the selected phage particles afforded a strong enzyme-linked immunosorbent assay (elisa) signal against gamma-irradiated b. mel ... | 2003 | 12738372 |
| spontaneous bacterial peritonitis due to brucella melitensis. | peritonitis is an extremely rare complication of brucellosis. a case is reported of blood and ascitic culture-proven spontaneous bacterial peritonitis caused by brucella melitensis, in a patient who had also cirrhosis. | 2003 | 12751716 |
| acute abdomen due to brucella melitensis. | a case of acute abdomen caused by a brucella melitensis is reported. the patient presented with biliary involvement in the form of acute acalculous cholecystitis and developed acute appendicitis that resulted in his surgical treatment. | 2003 | 12751720 |
| [sarcoidosis and brucellosis: a strange and infrequent association]. | a cattlewoman, a 63-year-old patient with sarcoidosis associated to mellitensis brucellosis, was studied. clinical manifestations of both multisystemic diseases were compared, as well as diagnostic and therapeutic aspects; we concluded that coexistence of both entities is infrequent but may occur due to increased presentation of the diseases. | 2003 | 12754952 |
| predicting gene expression levels from codon biases in alpha-proteobacterial genomes. | predicted highly expressed (phx) genes in five currently available high g+c complete alpha-proteobacterial genomes are analyzed. these include: the nitrogen-fixing plant symbionts sinorhizobium meliloti (sinme) and mesorhizobium loti (meslo), the nonpathogenic aquatic bacterium caulobacter crescentus (caucr), the plant pathogen agrobacterium tumefaciens (agrtu), and the mammalian pathogen brucella melitensis (brume). three of these genomes, sinme, agrtu, and brume, contain multiple chromosomes o ... | 2003 | 12775761 |
| brucellar spinal epidural abscess. | 2003 | 12776958 | |
| evaluation of a modified rose bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of brucella melitensis infection in sheep. | a modified rose bengal test (mrb) and an indirect elisa (ielisa) with protein g as the conjugate, were evaluated for the diagnosis of brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard rose bengal (rb) and complement fixation (cf) tests used in the current eradication campaign in eu countries. all tests showed 100% specificity when testing the sera from 212 brucella-free sheep. when testing ... | 2003 | 12791239 |
| persisting fever in a patient with brucella endocarditis: occult splenic abscess. | brucella endocarditis, despite its high mortality rate with combined medical and surgical treatment, has a low occurrence rate in cases of brucellosis and has been endemic in regions surrounding turkey. rarely, patients with infective endocarditis with common microorganisms develop a splenic abscess. a patient is reported on with brucella endocarditis and persistent fever. an occult splenic abscess was found. this is the second reported case in the literature of brucella endocarditis with spleni ... | 2003 | 12807868 |
| epitope mapping of the brucella melitensis bp26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. | sequencing of bp26, the gene encoding the brucella sp. immunogenic bp26 periplasmic protein, was performed in the reference strains of brucella abortus, b. suis, and b. ovis. the three bp26 sequences were almost identical to that published for b. melitensis 16m bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. the bp26 genes of the seven b. abortus biovar reference strains and b. abortus s19 and rb51 vaccine strains were also ... | 2003 | 12853399 |
| expression cloning and biochemical characterization of a rhizobium leguminosarum lipid a 1-phosphatase. | lipid a of rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, displays several significant structural differences when compared with escherichia coli. an especially striking feature of r. leguminosarum lipid a is that it lacks both the 1- and 4'-phosphate groups. distinct lipid a phosphatases that attack either the 1 or the 4' positions have previously been identified in extracts of r. leguminosarum and rhizobium etli but not sinorhizobium meliloti or e. coli. here we describe the id ... | 2003 | 12869541 |
| characterization of new members of the group 3 outer membrane protein family of brucella spp. | impairment of the omp25 gene in brucella spp. leads to attenuated strains and confers protection to the host. omp25 and omp31, whose functions remain unknown, were the first characterized members of group 3 outer membrane proteins (omps) (25 to 34 kda). recently, genomic and proteomic approaches identified five new putative members of this family, some of which are produced in b. melitensis or b. abortus. in the present study, using protein microsequencing, we identified new members of group 3 o ... | 2003 | 12874309 |
| biotypes and antimicrobial susceptibilities of brucella isolates. | 41 brucella strains isolated from blood and cerebrospinal fluid cultures were identified to species level and biotypes detected. all of the isolates were brucella melitensis: 2 strains of b. melitensis biotype-1 and 39 strains of b. melitensis biotype-3. in vitro activities of these strains were detected by the e test method. according to the 90% minimal inhibitory concentration (mic90) values, the most active agent was doxycycline (mic90 0.064 microg/ml), followed by ciprofloxacin (mic90 0.25 m ... | 2003 | 12875523 |
| spontaneous bacterial peritonitis caused by brucella melitensis. | this report presents a case of blood and ascitic fluid culture-proven spontaneous bacterial peritonitis caused by brucella melitensis in a 67-y-old male with congestive heart failure. ten cases of reported previously brucella peritonitis are reviewed, 3 of which were ascitic fluid culture negative. | 2003 | 12875525 |
| molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. | brucellosis caused by brucella spp. is a major zoonotic disease. control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. recently, brucella melitensis was declared by the centers for disease control and prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. also, the economic agricultural loss due to bovine brucellosis emphasizes the financial impact of bruce ... | 2003 | 12525425 |
| brucella melitensis endocarditis of ventricular septal defect patch: successful surgical treatment without replacement. | 2003 | 12539006 | |
| nramp1 is not a major determinant in the control of brucella melitensis infection in mice. | brucella, the causative agent of brucellosis in animals and humans, can survive and proliferate within macrophages. macrophages mediate mouse resistance to various pathogens through the expression of the nramp1 gene. the role of this gene in the control of brucella infection was investigated. when balb/c mice (nramp1(s)) and c.cb congenic mice (nramp1(r)) were infected with brucella melitensis, the number of brucella organisms per spleen was significantly larger in the c.cb mice than in the balb ... | 2003 | 12540538 |
| biosynthesis of phosphatidylcholine in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes and can be synthesized by either of two pathways, the methylation pathway or the cdp-choline pathway. many prokaryotes lack pc, but it can be found in significant amounts in membranes of rather diverse bacteria and based on genomic data, we estimate that more than 10% of all bacteria possess pc. enzymatic methylation of phosphatidylethanolamine via the methylation pathway was thought to be the only biosynthetic pat ... | 2003 | 12547654 |
| optimum detection times for bacteria and yeast species with the bactec 9120 aerobic blood culture system: evaluation for a 5-year period in a turkish university hospital. | we tracked and documented the time of positivity of blood cultures by using the bactec 9120 (becton dickinson diagnostic instrument systems) blood culture system over a 5-year study period. a 7-day protocol of the incubation period was selected, and a total of 11156 blood cultures were evaluated. the clinically significant microorganisms (32.95%) were isolated in 3676 specimens. gram-positive and -negative bacterial isolation rates were found to be 41.07 and 44.88%, respectively. yeasts were fou ... | 2003 | 12574291 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| epidemiological, clinical and laboratory features of brucellosis: a prospective evaluation of 120 adult patients. | this prospective study was carried out in the department of infectious diseases of gaziantep university between january 1997 and december 1999 to evaluate the epidemiological, clinical and laboratory features of brucellosis in south-eastern turkey. one hundred and twenty consecutive patients with active brucellosis were enrolled. the commonest way of transmission was ingestion of milk products from diseased animals. brucella melitensis was isolated in the specimens of 31 (45.5%) of 68 patients. ... | 2003 | 12587937 |
| comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. | pcr assay has been shown to be a promising option for the diagnosis of brucellosis. however, few studies have been performed with field samples in order to evaluate the assay as a diagnostic tool. in this study, routine use of a species-specific pcr assay previously developed for the identification of brucella cultures was assessed for the detection of brucella dna directly from the stomach contents of aborted sheep fetuses. the assay is based on the insertion sequence is711 in the brucella chro ... | 2003 | 12591206 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| antibodies to the cp24 protein of brucella melitensis lack diagnostic usefulness in ovine brucellosis. | the potential diagnostic usefulness of antibodies to the ribosome recycling factor of brucella melitensis (cp24) was assessed in sheep by an indirect elisa with purified recombinant cp24. sera from uninfected animals from the uk (n=44) and from local flocks (n=42), from sheep naturally infected with b. melitensis (n=12) or b. ovis (n=12), and from lambs (n=7) or pregnant ewes (n=6) vaccinated with b. melitensis rev-1, were assayed. high specific optical densities (od(with antigen) - od(without a ... | 2003 | 12636998 |
| the recombinant omp31 from brucella melitensis alone or associated with rough lipopolysaccharide induces protection against brucella ovis infection in balb/c mice. | immunogenicity and protective activity against brucella ovis of detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli, purified rough lipopolysaccharide from b. ovis (r-lps) and a mixture of romp31 extract and r-lps (romp31 extract + r-lps) were assessed in balb/c mice. the experimental vaccines were compared with a hot saline extract (hs extract) from b. ovis mainly composed of outer membrane proteins (omps) and r-lps, and known to be prote ... | 2003 | 12650766 |
| brucella prosthetic joint infection: a report of 3 cases and a review of the literature. | we report 3 cases of brucella melitensis infection of prosthetic hips and knees, and we summarize data about 4 cases reported in the literature. six of the 7 affected patients were men. the median duration from prosthesis implantation to the onset of symptoms was 38.7 months. five patients had only local symptoms. preoperative joint aspirates yielded negative culture results for 3 patients, and blood culture results were negative for 6 patients. excisional arthroplasty was the initial interventi ... | 2003 | 12652405 |
| therapeutic effect of spiramycin in brucellosis. | this study was undertaken to investigate the usefulness of spiramycin in treatment for brucellosis in an animal model. | 2003 | 12654065 |
| interactions between brucella melitensis and human phagocytes: bacterial surface o-polysaccharide inhibits phagocytosis, bacterial killing, and subsequent host cell apoptosis. | brucellae are gram-negative intracellular pathogens that survive and multiply within host phagocytic cells. smooth organisms present o-polysaccharides (ops) on their surface. the wboa gene, which codes for the enzyme glycosyl transferase, is essential for the assembly of o-chain in brucella. deletion of wboa in smooth, virulent b. melitensis 16m results in a rough mutant designated wrr51. unlike b. abortus, both smooth and rough strains of b. melitensis are resistant to complement-mediated killi ... | 2003 | 12654833 |
| brucella melitensis infection stimulates an immune response leading to kikuchi-fujimoto disease. | a 43-year-old greek cattler with a history of brucellosis three months previously for which he was treated with sulfonamides for three weeks, was admitted to the hospital complaining of fever, arthralgias, night sweats, painful cervical and axillary lymph nodes as well as a weight loss of 8 kg in the previous four months. since microbiological and serological studies did not give a specific diagnosis, an open cervical lymph node biopsy was performed. the histological examination revealed kikuchi ... | 2003 | 12655790 |
| [maculopapular eruption with fluctuating fever]. | 2003 | 12671590 | |
| brucella melitensis bacteremia in children: review of 62 cases. | brucella bacteremia is not uncommon in children living in endemic areas. reports on brucella bacteremia, however, are scarce. its clinical features and complications are unknown. this retrospective review describes the clinical and laboratory characteristics, the relapse rate, and response to different regimens of antimicrobials in children with brucella bacteremia over a 5-year period. antimicrobial susceptibility testing was performed on all isolates. data on 62 children with brucella bacterem ... | 2003 | 12678419 |
| brucellosis and the respiratory system. | brucellosis is a zoonotic disease that remains endemic worldwide. its clinical manifestations and focal complications are often troublesome in making a diagnosis. involvement of the respiratory system in brucellosis is an acknowledged but rare event that is only occasionally described in literature. we describe 37 cases of respiratory involvement during the course of brucellosis that presented as pneumonia, bronchopneumonia, pleural effusion with a predominance of monocytic or lymphocytic infilt ... | 2003 | 13130417 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| neurobrucellosis and a demonstration of its involvement in spinal roots via magnetic resonance imaging. | to present a neurobrucellosis case with spinal root involvement by means of magnetic resonance imaging (mri). | 2003 | 14504617 |
| anatomy of an exposure: a hospital lab's recovery of brucella melitensis. | 2003 | 14524145 | |
| cloning and expression of bartonella henselae sucb gene encoding an immunogenic dihydrolipoamide succinyltransferase homologous protein. | immunoscreening of a zap genomic library of bartonella henselae strain houston-1 expressed in escherichia coli resulted in the isolation of a clone containing 3.5 kb bamhi genomic dna fragment. this 3.5 kb dna fragment was found to contain a sequence of a gene encoding a protein with significant homology to the dihydrolipoamide succinyltransferase of brucella melitensis (sucb). subsequent cloning and dna sequence analysis revealed that the deduced amino acid sequence from the cloned gene showed ... | 2003 | 14524617 |
| [acute calculous cholecystitis associated with brucella melitensis]. | 2003 | 14525714 | |
| molecular characterization of brucella abortus chromosome ii recombination. | large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. the recently completed brucella melitensis 16m and brucella suis 1330 genomes have facilitated the investigation of such events in the brucella spp. suppressive subtractive hybridization (ssh) was employed in identifying genomic differences between b. melitensis 16m and brucella abortus 2308. analysis of 45 ssh clones revealed several deletions on chromosomes of b. abortus ... | 2003 | 14526025 |
| acute brucellosis presenting with erythema nodosum. | brucellosis is a common world-wide zoonotic disease. cutaneous manifestations are not specific and affect 1-14% of patients with brucellosis. we describe two cases of young males presenting with skin lesions of erythema nodosum on the anterior surface of the legs. histopathology of skin biopsy revealed septal panniculitis, but the positive cultures of blood or bone marrow for brucella melitensis established the diagnosis of brucellosis. | 2003 | 14561053 |
| human mammary abscess caused by brucella melitensis: a case report. | 2003 | 14563232 | |
| activation and proliferation of t lymphocyte subpopulations in patients with brucellosis. | t-cell proliferation is a standard method to evaluate cellular immune responses against intracellular infectious agents. the present study was undertaken to look for expression of an early activation marker (cd69) and proliferation using a nonradioactive method to evaluate cellular immune response against a salt-extractable antigen from brucella melitensis 16m (rcm-bm) in patients suffering from brucellosis. | 2003 | 14567397 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| ovarian abscess due to brucella melitensis. | the case of a 25-y-old woman with brucellar ovarian abscess is reported. cultures of blood, ascites and a pus specimen yielded brucella melitensis. the possibility of ovarian abscess being caused by brucella melitensis should be considered in countries where the infection is endemic. | 2003 | 14606619 |
| spontaneous bacterial peritonitis due to brucella infection. | brucella infection is a systemic disease, but the microorganism rarely causes infections in the gastrointestinal system such as hepatitis, cholecystitis, colitis and pancreatitis. spontaneous bacterial peritonitis due to brucella is extremely rare. herein, we report a case of cirrhosis complicated with nongranulomatous hepatitis and peritonitis, both due to brucella. a 63 year-old man with diabetes mellitus was admitted to hospital with complaints of weakness, backache, abdominal pain and abdomi ... | 2003 | 14614644 |
| brucella spondylitis and sacroiliitis in the general population in mumbai. | 1) to identify patients of brucella spondylitis and sacroiliitis amongst patients of 'infective spondylitis' and 'sacroiliitis', 2) to study clinical and radiographic features and outcome of brucella spondylitis. | 2003 | 14621032 |
| attenuated signature-tagged mutagenesis mutants of brucella melitensis identified during the acute phase of infection in mice. | for this study, we screened 1,152 signature-tagged mutagenesis mutants of brucella melitensis 16m in a mouse model of infection and found 36 of them to be attenuated in vivo. molecular characterization of transposon insertion sites showed that for four mutants, the affected genes were only present in rhizobiaceae. another mutant contained a disruption in a gene homologous to mosa, which is involved in rhizopine biosynthesis in some strains of rhizobium, suggesting that this sugar may be involved ... | 2003 | 14638795 |
| the effects of streptozotocin-induced diabetes on brucellosis of rats. | it is believed that an infection is more common and runs a more protracted course in people with diabetes. in clinical practice, it is important to be aware of these associations, as the prognosis is often dependent upon prompt recognition and appropriate treatment. to show the course of brucellosis in the diabetic state, a model of brucella melitensis infection was used in the setting of streptozotocin-induced diabetes in rat. b. melitensis infection proceeded more severely in diabetic rats and ... | 2003 | 14642313 |
| neglected case of osteoarticular brucella infection of the knee. | a 49-year-old farmer had a history of recurrent knee effusion for 20 years. he did not report undergoing any diagnostic or therapeutic procedures apart from repeated aspirations of the joint fluid. after the isolation of brucella melitensis from the joint fluid, computed tomography-guided bone biopsy was performed and histopathologic examination of the biopsy sample confirmed the diagnosis of chronic brucella osteomyelitis. arthroscopic synovectomy combined with antimicrobial therapy with doxycy ... | 2003 | 14652892 |
| pathways for phosphatidylcholine biosynthesis in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes with important structural and signalling functions. although many prokaryotes lack pc, it can be found in significant amounts in membranes of rather diverse bacteria. two pathways for pc biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase (pcs) pathway. in the methylation pathway, phosphatidylethanolamine is methylated three times to yield pc, in reactions catalysed by o ... | 2003 | 14663079 |
| the effect of levamisole combined with the classical treatment in chronic brucellosis. | levamisole is an immunopotenciator drug which is used as an antihelmintic drug as well as very effective remedy on cellular immunity compared with humoral immunity. a total 71 patients (37 men, 34 women) who referred to our department between march 1997 and december 2001, with a history of the disease for about 1 year, were diagnosed as having chronic brucellosis through those tests brucella serum agglutination test (sat), sat with coombs and sat with 2-mercaptoethanol. the patients were randoml ... | 2003 | 14690014 |
| statistical procedures for calculating the residual virulence of brucella abortus strain 19 (s19) and brucella melitensis strain rev 1 vaccines in mice: theoretical basis and practical applications. | regular control of the biological quality of live brucella abortus strain 19 (s19) and b. melitensis strain rev 1 vaccines is essential for the successful management of ruminant brucellosis in affected countries. the reference procedures recommended by the oie (world organisation for animal health) and the european pharmacopoeia include the determination of residual virulence, expressed as the recovery time 50 (rt50), of the tested (problem) vaccine in a reference mouse model compared with the r ... | 2003 | 15005562 |
| guillain-barré syndrome associated with acute neurobrucellosis. | we report the case of a 14-year-old girl with guillain-barré syndrome associated with brucellosis due to brucella melitensis. the diagnosis was established by the isolation of b. melitensis from her blood and by the determination of high levels of brucella aglutinins in her sera and cerebrospinal fluid. a combination of rifampin, co-trimoxazole and physical therapy resulted in complete healing within 30 days. antibrucellar treatment continued for 12 weeks. this case report suggests that brucello ... | 2003 | 14712899 |
| brucellosis as a cause of prolonged fever in an era of pasteurized milk. | 2003 | 15260406 | |
| interaction of bacterial endotoxine (lipopolysaccharide) with latex particles: application to latex agglutination immunoassays. | the latex agglutination immunoassay technique uses polymer colloids as carriers for antibodies or antigens to enhance the immunological reaction. in this work, the interaction of a lipopolysaccharide (lps) of brucella melitensis with two conventional latexes has been studied. some experiments on the physical adsorption of the lps onto these polystyrene beads have been performed and several complexes with different coverage degrees were obtained by modifying the incubation conditions. regarding t ... | 2002 | 16290356 |
| a brucellosis case with ascites, hearing loss and pancytopenia. | to date there has been no association of ascites, hearing loss and pancytopenia in brucellosis in the literature. a 25-year-old female patient was hospitalized with fever, hearing loss, ascites and pancytopenia. cultures from bone marrow and ascites yielded growth of brucella melitensis and brucella standard tube agglutination was found to be positive at a titer of 1/1280. the patient completely recovered by the sixth week following combined antibacterial treatment of ciprofloxacin, rifampin and ... | 2002 | 16378300 |
| endemic brucellar epididymo-orchitis: a 10-year experience. | to present epidemiologic, clinical and laboratory features, treatment and outcome of patients suffering from brucella melitensis-induced epididymo-orchitis, in comparison with cases of nonspecific epididymo-orchitis. distinction between these two entities is essential, as treatment and outcome are entirely different. | 2002 | 12718826 |
| brucellosis in children: clinical observations in 115 cases. | brucellosis is endemic in saudi arabia. this report summarizes the epidemiology of brucellosis in children. | 2002 | 12718832 |
| [ciprofloxacin in the treatment of patients with brucellosis]. | with the aim to estimate the clinical and immunological efficiency of the ciprofloxacin (cifloxinal) 105 patients with acute (51), subacute (19) and chronic (35) brucellosis were studied. control group (17 patients with acute and 30 patients with chronic brucellosis) have been treated with combination of two antibiotics: doxycycline and rifampicin. ciprofloxacin in a dose 500 mg bid within 14 days in acute stage and 20 days in chronic stage of disease essentially reduced duration of local inflam ... | 2002 | 12674788 |
| [evaluation of the methods used for the serologic diagnosis of brucellosis]. | serological tests are the most widely used methods for the diagnosis of brucella infections, since bacterial isolation techniques are time consuming. the conventional serological methods are the tube and slide agglutination tests, however enzyme immunoassays (elisa) have gained importance recently as rapid diagnostic techniques. the aim of this study was to evaluate the performances of standard tube agglutination (sta, wright test), slide agglutination (rose-bengal, rb) and elisa-igg and -igm me ... | 2002 | 12652868 |
| late reactivation of calcified granuloma in a patient with chronic suppurative brucellosis. | 2002 | 12525929 | |
| prediction and overview of the rpon-regulon in closely related species of the rhizobiales. | in the rhizobia, a group of symbiotic gram-negative soil bacteria, rpon (sigma54, sigman, ntra) is best known as the sigma factor enabling transcription of the nitrogen fixation genes. recent reports, however, demonstrate the involvement of rpon in other symbiotic functions, although no large-scale effort has yet been undertaken to unravel the rpon-regulon in rhizobia. we screened two complete rhizobial genomes (mesorhizobium loti, sinorhizobium meliloti) and four symbiotic regions (rhizobium et ... | 2002 | 12537565 |
| split personalities. | 2002 | 11864813 | |
| diagnostic usefulness of antibodies against ribosome recycling factor from brucella melitensis in human or canine brucellosis. | the diagnostic usefulness of an enzyme-linked immunosorbent assay (elisa) using a purified recombinant ribosome recycling factor from brucella melitensis (cp24 antigen) was tested in human and canine infections caused by smooth and rough brucella species, respectively. anti-cp24 antibodies were detected in 9 (43%) of 21 consecutive cases of canine brucellosis and in 8 (53%) of 15 dogs followed for 60 days after the diagnosis of acute brucellosis. among eight patients with acute brucellosis, anti ... | 2002 | 11874879 |
| isolation of brucella melitensis from an arabian oryx (oryx leucoryx). | 2002 | 11888113 | |
| brucellosis in the etiology of febrile neutropenia: case report. | brucellosis is one of the leading diseases in the differential diagnosis of fever of unknown origin in some parts of the world. it can lead to treatment failure because of slow growth in blood cultures and late appearance of signs and symptoms in patients with febrile neutropenia who were unresponsive to empirical antibiotic treatment. during the last year in our oncology unit adjuvant chemotherapy was given to 3 patients with breast (n=1) and stomach cancer (n=2) and febrile neutropenia was see ... | 2002 | 11892906 |
| splenic infarction associated with acute brucella mellitensis infection. | 2002 | 11913506 | |
| identification of the brucella melitensis vaccine strain rev.1 in animals and humans in israel by pcr analysis of the psti site polymorphism of its omp2 gene. | adverse effects of strain persistence and secretion in milk have been encountered with the brucella melitensis vaccine strain rev.1. field isolates obtained from vaccinated animals and from a human resembled the vaccine strain rev.1 by conventional bacteriological tests. the lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the rev.1-like field isolates to the vaccine strain. the composition of the omp ... | 2002 | 11923376 |
| medically treated splenic abscess due to brucella melitensis. | brucellosis may lead to complications that affect different organ systems, including the liver and spleen. in acute disease, hepatosplenic abscess is a rare complication. we report herein a woman with splenic abscess due to acute brucella melitensis infection who was successfully treated with antibiotics alone. | 2002 | 11928846 |
| chronic hepatosplenic abscesses in brucellosis. clinico-therapeutic features and molecular diagnostic approach. | in order to analyze the clinical and therapeutic features of chronic hepatosplenic abscesses, and to define the diagnostic yield of new molecular techniques, we describe seven cases, four hepatic and three splenic, of this uncommon complication of brucellosis. onset of symptoms in all cases was insidious and the diagnostic delay considerable. abdominal ct scan showed large, poorly defined lesions, with heterogeneous attenuation and thick central calcifications surrounded by hypointense areas. hi ... | 2002 | 11929686 |
| combined brucella melitensis and streptococcal viridans endocarditis. | infectious endocarditis is a potentially lethal inflammation of the hearts' inner lining invaded by microorganisms. the mortality from this illness increases as the number of infective organisms rises to 2, due to involvement of the left side of the heart. these microorganisms usually arise from the patient's own flora but can be acquired from the environment. fever and heart murmurs are the principal clinical manifestations followed by a plethora of peripheral signs due to dissemination of micr ... | 2002 | 11938375 |
| brucellosis as a cause of septic loosening of total hip arthroplasty. | infection after a total hip arthroplasty is a severe complication and is associated with a high incidence of morbidity. we describe a case of late infection, 5 years after the implantation of a cemented hip prosthesis. the infection was caused by gram-negative brucella melitensis and occurred in a 63-year-old man who owned cattle. as far as we know, such a complication has never been published before. | 2002 | 11938520 |
| [analysis of immune response of guinea pigs infected with brucella melitensis]. | the dynamics of the first antigen specific stage of immune response to brucella infection was experimentally studied with the method of binding adsorbed antigenic immunoreagents with lymphocytes. the study revealed that the content of antigen-binding lymphocytes (abl) reached its maximum as early as on day 7 after infection, gradually decreasing afterwards (but even on day 90 abl could be detected in the blood). the specificity of abl was proved by the fact that they were absent in noninfected a ... | 2002 | 11949255 |
| the genome sequence of the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and malta fever in humans. the genome of b. melitensis strain 16m was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 orfs. by using the bioinformatics suite ergo, 2,487 (78%) orfs were assigned functions. the origins of replication of the two chromosomes are similar to those of other alpha-proteobacter ... | 2002 | 11756688 |
| brucella melitensis: a nasty bug with hidden credentials for virulence. | 2002 | 11782541 | |
| epididymo-orchitis caused by brucella melitensis in breeding rams in india. | 2002 | 11837594 | |
| study of interleukin-10 and interleukin-12 productions in response to lipopolysaccharides extracted from two different brucella strains. | the aim of the present study is to investigate the cytokines induction by smooth lipopolysaccharides (s-lps) extracted from brucella melitensis (rev1 vaccine strain) and brucella abortus (a field isolate). these lipopolysaccharides were used to induce inflammatory cytokines production in peripheral blood cell culture of healthy individuals. secretion of il-10 and il-12 (p70) were measured by means of specific elisa. in addition, intracellular expression of il-12 was assessed in cd14+ cells by fl ... | 2002 | 11848132 |
| analysis of sampling strategies to substantiate freedom from disease in large areas. | in this paper, we deal with the strategies of surveys to substantiate freedom from disease for a certain territory. infection might not be distributed homogeneously. so, a relatively high within-herd prevalence might be observed while the herd-level prevalence is lower. for this situation, we compare various two-stage sample strategies. the calculation of appropriate sample sizes becomes quite complicated. the theoretical generalization of the hypergeometric distribution by cameron and baldock [ ... | 2002 | 11849726 |
| biological warfare: implications for antimicrobial use. | biological warfare is intended to incapacitate a large number of individuals at a single exposure, creating epidemic-type disease, death, and social chaos. the organisms with potential for immediate use as bacteriologic weapons are bacillus anthracis, brucella melitensis, yersinia pestis, and vibrio cholera, all necessitating antibiotic therapy for a cure. it is reasonable, therefore, to assume that a biological attack, or even a hoax, would requiure thousands of individuals over a large area to ... | 2002 | 11853654 |
| immunogenicity of the brucella melitensis recombinant ribosome recycling factor-homologous protein and its cdna. | a study was conducted to evaluate the immunogenicity of the brucella melitensis ribosome recycling factor (rrf)-homologous protein (cp24). the cp24 gene was cloned, expressed in escherichia coli and purified. the resulting purified recombinant protein (rcp24) produced delayed-type hypersensitivity (dth) reactions in b. melitensis-infected mice but not in naive controls. thus, we decided to characterise the immune responses generated with dna vaccination (pcdnacp24) or immunisation with the rcp24 ... | 2002 | 11858876 |
| spinal brucellosis: case report in the united states. | case report. | 2002 | 11979184 |
| [diagnosis of human brucellosis. role of ph in the seroagglutination test and influence of ph on the agglutinating activity of igm, igg and iga antibodies]. | to evaluate the role of ph in the seroagglutination test (sat)and rose bengal (rb) test, and to determine the influence of ph on the agglutinating activity of igm, igg and iga antibodies. | 2002 | 11996698 |
| identification of a quorum-sensing signal molecule in the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. a dichloromethane extract of spent culture supernatant from b. melitensis induces bioluminescence in an escherichia coli acyl-homoserine lactone (acyl-hsl) biosensor strain based upon the activity of the lasr protein of pseudomonas aeruginosa. hplc fracti ... | 2002 | 12010991 |
| expression cloning and characterization of the c28 acyltransferase of lipid a biosynthesis in rhizobium leguminosarum. | an unusual feature of lipid a from plant endosymbionts of the rhizobiaceae family is the presence of a 27-hydroxyoctacosanoic acid (c28) moiety. an enzyme that incorporates this acyl chain is present in extracts of rhizobium leguminosarum, rhizobium etli, and sinorhizobium meliloti but not escherichia coli. the enzyme transfers 27-hydroxyoctacosanate from a specialized acyl carrier protein (acpxl) to the precursor kdo2 ((3-deoxy-d-manno-octulosonic acid)2)-lipid iv(a). we now report the identifi ... | 2002 | 12019272 |
| brucella peritonitis in a patient on continuous ambulatory peritoneal dialysis with acute brucellosis. | peritonitis is an uncommon complication of brucellosis. brucella peritonitis in chronic ambulatory peritoneal dialysis (capd) patients has not been reported before. a male patient is presented with peritonitis caused by brucella melitensis who was on capd. the source of infection was thought to be unpasteurized, unsalted cheese eaten a month before the onset of symptoms. at the beginning, antibiotic therapy with doxycyline and rifampicin led to a rapid clinical improvement, with disappearance of ... | 2002 | 12021533 |
| in vitro activities of new quinolones against brucella melitensis isolated in a tertiary-care hospital in turkey. | we have evaluated the in vitro activities of seven fluoroquinolones against 69 strains of brucella melitensis. according to their minimum inhibitory concentration for 90% growth (mic(90)) values, the most active agent was found to be sparfloxacin (mic(90) 0.12 mg/l) followed by levofloxacin, ciprofloxacin, ofloxacin (mic(90) 0.50 mg/l) and grepafloxacin (mic(90) 1 mg/l), gemifloxacin (mic(90) 2 mg/l) and gatifloxacin (mic(90) 4 mg/l). | 2002 | 12047416 |
| clinical microbiological case: a 22-year-old-man with fever and maculopapular rash. | 2002 | 12047418 | |
| identification of brucella melitensis vaccine strain rev.1 by pcr-rflp based on a mutation in the rpsl gene. | the live attenuated strain b. melitensis rev.1 is considered the best vaccine available for the prophylaxis of brucellosis in sheep and goats. the rev.1 vaccine was obtained in the 1950s by a two-step selection involving firstly streptomycin resistance and dependence and secondly reversion of dependence but keeping streptomycin resistance. chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein s12, rpsl. nucleotide sequencing reve ... | 2002 | 12057611 |
| protection of mice against brucellosis by intranasal immunization with brucella melitensis lipopolysaccharide as a noncovalent complex with neisseria meningitidis group b outer membrane protein. | intranasal immunization of mice with purified brucella melitensis lipopolysaccharide (lps) as a noncovalent complex with neisseria meningitidis group b outer membrane protein (gbomp) elicited a high-titer anti-lps systemic antibody response and a significant mucosal antibody response. the anti-lps immunoglobulin g (igg) antibody was predominantly of the igg1 subtype, although there was some response of the igg2a, igg2b, and igg3 subtypes. the antibody titer remained high for 16 weeks postimmuniz ... | 2002 | 12065469 |
| pathogenicity and protective activity in pregnant goats of a brucella melitensis deltaomp25 deletion mutant. | the brucella melitensis mutant bm 25, which lacks the major 25 kda outer membrane protein omp25, has previously been found to be attenuated in the murine brucellosis model. in the present study, the capacity of the deltaomp25 mutant to colonise and cause abortions in the caprine host was evaluated. the vaccine potential of bm 25 was also investigated in goats. inoculation of nine pregnant goats in late gestation with the b. melitensis mutant resulted in 0/9 abortions, while the virulent parental ... | 2002 | 12076120 |
| bacteria-modified amperometric immunosensor for a brucella melitensis antibody assay. | a novel amperometric immunosensor setup is described which uses horseradish peroxidase (hrp) as a label in conjunction with a current-based brucella sensor. the bacteria modified immunosensor was constructed by using a biocomposite formed by dispersing graphite powder into a mixture of brucella melitensis and silicate polymer gel. the enzyme-labeled antibody can readily diffuse toward the encapsulated antigen (brucella melitensis), which retains its binding properties, and the association reacti ... | 2002 | 12083544 |
| branchial cyst empyema due to brucella melitensis infection as a form of focal brucellosis. | 2002 | 12099739 | |
| leukocyte count in the synovial fluid of children with culture-proven brucellar arthritis. | brucellosis is an important cause of paediatric septic arthritis in endemic areas. because the gram stain is frequently negative and culture results are unavailable at the time of the patient's admission, the diagnosis of brucellar arthritis is usually entertained on the bases of epidemiological considerations and cytological examination of the synovial fluid aspirate. the aim of this study was to assess the sensitivity of a synovial fluid leukocyte count >50 000 wbc/mm(3) for detecting culture- ... | 2002 | 12111621 |
| fever of unknown origin in a previously healthy child. | 2002 | 12118845 | |
| comparison between immune responses and resistance induced in balb/c mice vaccinated with rb51 and rev. 1 vaccines and challenged with brucella melitensis bv. 3. | balb/c mice were immunized with live rough brucella abortus rb51 or smooth brucella melitensis rev. 1 vaccines and challenged with a b. melitensis field strain. protection was assessed by a variety of serological tests and recovery of vaccinal and challenge strains by culture. mice vaccinated with rb51 gave negative results in the conventional serological tests prior to challenge, namely; standard tube agglutination test (sat), rose bengal plate test (rbpt), buffered acidified plate antigen test ... | 2002 | 12119140 |
| brucellar orchitis in innerwest anatolia region of turkey. a report of 12 cases. | brucellosis, which affects the genitourinary system in rate of 2-20%, is a multiorgan infectious disease. twelve patients were diagnosed as having brucellar orchitis serologically, clinically and ultrasonographically. in 2 patients, brucella melitensis was isolated in blood cultures. all the patients were working in cattle dealing. they were treated with 600 mg/day rifampicin plus 200 mg/day doxycycline for 6 weeks and followed up during 1 year. they recovered clinically within 3 weeks. although ... | 2002 | 12119436 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 22-2002. a 37-year-old man with unexplained fever after a long trip through south america. | 2002 | 12124410 | |
| brucella species lacking the major outer membrane protein omp25 are attenuated in mice and protect against brucella melitensis and brucella ovis. | to aid in the development of novel efficacious vaccines against brucellosis, omp25 was examined as a potential candidate. to determine the role of omp25 in virulence, mutants were created with brucella abortus (ba25), brucella melitensis (bm25), and brucella ovis (bo25) which contain disruptions in the omp25 gene (deltaomp25 mutants). western immunoblot analysis and pcr verified that the omp25 protein was not expressed and that the omp25 gene was disrupted in each strain. balb/c mice infected wi ... | 2002 | 12151196 |
| brucella sp. bind to sialic acid residues on human and animal red blood cells. | we report that brucella abortus and brucella melitensis agglutinate human (a+ and b+), hamster and rabbit erythrocytes, a heretofore undescribed feature in this genus. this activity was associated with a 29-kda surface protein (sp29) that bound selectively to these erythrocytes and this binding was inhibited by rabbit anti-sp29 antibodies. hemagglutination was inhibited by pretreatment of erythrocytes with neuraminidase and by preincubation of b. abortus with chondroitin sulfate, n-acetylneurami ... | 2002 | 12167541 |
| antigen-specific activation and proliferation of cd4+ and cd8+ t lymphocytes from brucellosis patients. | salt-extractable antigen from brucella melitensis 16m (rcm-bm) was used to evaluate the immune response from acute and chronic patients suffering from brucella infections (in mexico); their responses were compared with those of healthy controls. as a readout we used upregulation of cd69 (a well-established early activation marker for lymphocytes), lymphocyte proliferation by 3[h]thymidine or 5-bromo-2-deoxyuridine (brdu) incorporation measured by liquid scintillation or flow cytometry, respectiv ... | 2002 | 12174793 |
| the yersinia pseudotuberculosis yut protein, a new type of urea transporter homologous to eukaryotic channels and functionally interchangeable in vitro with the helicobacter pylori urei protein. | urea uptake in eukaryotes and prokaryotes occurs via diffusion or active transport across the cell membrane. facilitated diffusion of urea in both types of organisms requires a single-component channel. in bacteria, these transport systems allow rapid access of urease to its substrate, resulting in ammonia production, which is needed either for resistance to acidity or as a nitrogen source. in yersinia pseudotuberculosis, a ureolytic enteropathogenic bacterium, a gene of unknown function (yut) l ... | 2002 | 12180933 |