Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| bacterial diversity in human subgingival plaque. | the purpose of this study was to determine the bacterial diversity in the human subgingival plaque by using culture-independent molecular methods as part of an ongoing effort to obtain full 16s rrna sequences for all cultivable and not-yet-cultivated species of human oral bacteria. subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16s ribos ... | 2001 | 11371542 |
| smoking affects the subgingival microflora in periodontitis. | tobacco smoking has been identified as one major risk factor for destructive periodontal disease. scaling and root planing have been shown to be less effective in smokers with periodontitis. the aim of the present study was to compare the subgingival microbial flora of treated and untreated smokers and non-smokers. | 2001 | 11394403 |
| development of a gene inactivation system for bacteroides forsythus: construction and characterization of a bspa mutant. | bacteroides forsythus is a gram-negative anaerobic bacterium associated with periodontitis. the bspa gene encoding a cell surface associated leucine-rich repeat protein (bspa) involved in adhesion to fibronectin and fibrinogen was recently cloned from this bacterium in our laboratory. we now describe the construction of a bspa-defective mutant of b. forsythus. this is the first report describing the generation of a specific gene knockout mutant of b. forsythus, and this procedure should be usefu ... | 2001 | 11402017 |
| change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole. | the current investigation evaluated changes in levels and proportions of 40 bacterial species in subgingival plaque samples during, immediately after and up to 1 year after metronidazole or amoxicillin therapy combined with srp. | 2001 | 11422580 |
| untreated periodontal disease in indonesian adolescents. subgingival microbiota in relation to experienced progression of periodontitis. | in an indonesian population deprived of regular dental care, the experienced progression of disease between baseline (1987) and follow-up (1994) was investigated in relation to the composition of the subgingival microbiota at follow-up. at baseline the age ranged from 15 to 25 years. clinical and microbiological evaluation was completed in 158 of the 167 subjects available at follow-up. | 2001 | 11422582 |
| [bacteriologic investigation of periodontal infections]. | the gingivitis and periodontitis are very common diseases in hungary: more than 80% of the adult population suffers with gingivitis and 15-20% has destructive periodontitis. the composition of peridontopathogenic bacterial flora is totally different from that of the bacterial flora causing dental infections. in periodontal infections dominantly facultative and obligatory anaerobic microorganisms occur (actinobacillus actinomycetemcomitans (a. actinomycetemcomitans), porphyromonas gingivalis (p. ... | 2001 | 11480243 |
| treatment of peri-implantitis by local delivery of tetracycline. clinical, microbiological and radiological results. | the purpose of this study was to investigate the clinical, microbiological and radiological effects of peri-implantitis therapy by local delivery of tetracycline. in 25 partially edentulous patients, 30 implants with radiographic evidence of circumferential bone loss, and peri-implant probing depths > or =5 mm were treated with polymeric tetracycline hcl-containing fibers. clinical and microbial parameters were recorded at baseline, and 1, 3, 6, and 12 months (m) after treatment. standardized ra ... | 2001 | 11488856 |
| clinical and microbiological evaluation of ligature-induced peri-implantitis and periodontitis in dogs. | the purpose of this study was to evaluate the attachment loss around teeth and implants by clinical and microbiological analysis. the mandibular premolars were extracted in 5 mongrel dogs and, 3 months later, two titanium implants were installed on each side of the mandible and, after another 3 months, abutment connection was performed. plaque control in the implants and maxillary premolars was maintained for two weeks prior to the start of the main experiment. on day 0 and 30 days after ligatur ... | 2001 | 11488857 |
| herpes viruses and periodontopathic bacteria in early-onset periodontitis. | this study examined the occurrence of human herpes viruses and suspected periodontopathic bacteria in early-onset periodontitis patients who experienced progressive disease in at least 2 periodontal sites during the maintenance phase of therapy. | 2001 | 11493359 |
| clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene. | this is the first known case report that used a polymerase chain reaction (pcr)-based method to help identify the oral microflora in patients with chronic neutropenia. in this study, we report clinical periodontal findings and microflora profiles of 2 children, 1 with severe congenital neutropenia (scn, kostmann type) and 1 with cyclic neutropenia (cn). | 2001 | 11495144 |
| humoral immune response in early-onset periodontitis: influence of smoking. | sixty-five patients with generalised early-onset periodontitis (g-eop) (age range 16-42 years, 32 smokers and 33 non-smokers) were assessed for antibody titres and avidity to a panel of five suspected periodontal pathogens (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, treponema denticola and bacteroides forsythus). thirty-four of these patients were untreated (17 smokers and 17 non-smokers), and thirty-one were in the maintenance phase of periodontal the ... | 2001 | 11519695 |
| mixed infection of porphyromonas gingivalis and bacteroides forsythus in a murine abscess model: involvement of gingipains in a synergistic effect. | several microorganisms including porphyromonas gingivalis and bacteroides forsythus have been implicated to be etiologically important agents of periodontal disease. in this study, we determined the ability of combinations of periodontopathogenic microorganisms to cause tissue destruction in a murine abscess model. although all bacterial combinations used in this study produced larger abscesses than did monoinfection of each bacterium, the combination of p. gingivalis and b.forsythus showed a sy ... | 2001 | 11519697 |
| identification of oral spirochetes at the species level and their association with other bacteria in endodontic infections. | recent molecular approaches have revealed that fastidious organisms such as bacteroides forsythus and oral treponemes were frequently found in root canals with apical periodontitis. the purpose of this study was to identify the isolates of oral spirochetes at the species level in endodontic infections and to determine their association with b forsythus and porphyromonas gingivalis. | 2001 | 11552154 |
| the opdb locus encodes the trypsin-like peptidase activity of treponema denticola. | high levels of treponema denticola in subgingival dental plaque are associated with severe periodontal disease. t. denticola, along with porphyromonas gingivalis and bacteroides forsythus, are the only cultivatable oral microorganisms that produce significant amounts of "trypsin-like" peptidase activity. the ability of subgingival plaque to hydrolyze n-alpha-benzoyl-dl-arginine-2-naphthylamide (bana) is associated with high levels of one or more of these organisms. the purpose of this study was ... | 2001 | 11553560 |
| salivary albumin, total protein, iga, igg and igm concentrations and occurrence of some periodontopathogens in hiv-infected patients: a 2-year follow-up study. | hiv infection reduces oral defensive mechanisms and may affect mucosal integrity. differences in salivary protein concentrations and periodontopathogenic bacteria were studied in 56 hiv-infected patients with respect to their disease phase. thirty-three patients were followed up for 2 years. fifty-three healthy subjects of corresponding age and sex were studied as controls. at baseline, salivary albumin, total protein, iga, and igm levels were significantly higher (p<0.05-0.0001) in all phases o ... | 2001 | 11555159 |
| immune response to bacteroides forsythus in a murine model. | a murine skin abscess model was used to study the immune response to an acute infection with bacteroides forsythus. balb/c mice were given subcutaneous injections of either viable or heat-killed b. forsythus, while a third sham-immunized control group received phosphate-buffered saline. weights and lesion sizes were measured. blood was collected from the heart and specific antibodies to b. forsythus measured by an elisa. swabs taken from the lesions and also from pooled blood were cultured anaer ... | 2001 | 11555309 |
| periodontal infections contribute to elevated systemic c-reactive protein level. | periodontitis is a local inflammatory process mediating destruction of periodontal tissues triggered by bacterial insult. however, this disease is also characterized by systemic inflammatory host responses that may contribute, in part, to the recently reported higher risk for cardiovascular disease (cvd) among patients with periodontitis. moderate elevation of c-reactive protein (crp) has been found to be a predictor of increased risk for cvd. elevated crp levels in periodontal patients have bee ... | 2001 | 11577954 |
| periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. | periodontal disease is perhaps the most common chronic infection in adults. evidence has been accumulating for the past 30 years which indicates that almost all forms of periodontal disease are chronic but specific bacterial infections due to the overgrowth in the dental plaque of a finite number of mostly anaerobic species such as porphyromonas gingivalis, bacteroides forsythus, and treponema denticola. the success of traditional debridement procedures and/or antimicrobial agents in improving p ... | 2001 | 11585783 |
| microbiological evaluation of acute periradicular abscesses by dna-dna hybridization. | the purpose of the present investigation was to examine the microbiota of acute periradicular abscesses of endodontic origin by using a molecular genetic method. | 2001 | 11598583 |
| efficacy of manual and powered toothbrushes (ii). effect on microbiological parameters. | the purpose of the present investigation was to determine the effect of self-performed supragingival plaque removal using either manual (crest complete) or power (braun 3d plaque remover) toothbrushing on supra and subgingival plaque composition. | 2001 | 11686813 |
| absence of a specific subgingival microflora in adults with down's syndrome. | periodontal disease in down's syndrome (ds) is generally characterized by a high degree of bone loss. bone loss of 5 mm or more is observed in 70% of these subjects. among ds subjects, considerable differences in disease progression occur. so far, no studies have been conducted in which specific properties of the subgingival microflora have been related to the condition observed. | 2001 | 11686820 |
| [bacteroides forsythus: sensitivity to antimicrobial agents in samples from patients with periodontitis]. | an in vitro antimicrobial sensitivity test (technique of agar dilution) was carried out for 105 clinical isolates of b. forsythus from patients with periodontitis. metronidazole and amoxicillin were the most efficient drugs and, thus, are indicated for the treatment of periodontal infections in which this microorganism is the most prevalent pathogen. | 2001 | 11708285 |
| association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. | the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions. | 2001 | 11737506 |
| sulfate-reducing bacteria in relation with other potential periodontal pathogens. | oral sulfate-reducing bacteria are involved in several clinical categories of periodontitis. the aim of this cross-sectional study was to compare the presence of sulfate-reducing bacteria (srb) with other putative pathogens including spirochetes, actinobacillus actinomycetemcomitans, bacteroides forsythus, porphyromonas gingivalis, and treponema denticola in periodontal lesions. | 2001 | 11737513 |
| supragingival and subgingival microbiota of adult patients with down's syndrome. changes after periodontal treatment. | in this longitudinal study, five adult down's syndrome patients with periodontitis were placed on a frequent recall visit schedule (every 6 weeks) after treatment, in order to investigate: 1) the microbiological status, both supragingivally and subgingivally, and the changes that occurred after treatment and 2) the effect of frequent professional supragingival plaque control on the subgingival microbiota and clinical variables in these patients. the clinical variables recorded were probing pocke ... | 2001 | 11737662 |
| bacteroides forsythus prth genotype in periodontitis patients: occurrence and association with periodontal disease. | bacteroides forsythus has been described as a periodontopathogen and its presence in the subgingival plaque can lead to periodontal disease. recently, a cysteine protease designated as prth was isolated and characterized from b. forsythus atcc 43037. the purpose of this study was to determine the prevalence and the association of the prth gene of b. forsythus with periodontal disease. a total of 160 subgingival plaque samples were assayed with the polymerase chain reaction method using oligonucl ... | 2001 | 11762876 |
| one-year compliance and effects of amine and stannous fluoride on some salivary biochemical constituents and oral microbes in institutionalized elderly. | due to old age and poor general health, the institutionalized elderly have often not been used as research subjects for the investigation of oral health protocols. because the number of elderly is increasing, they are an important target group for the development of oral hygiene aids. this trial was designed to investigate whether the institutionalized elderly could successfully use an amine-fluoride- and stannous-fluoride-containing mouthwash and toothpaste during a 12-month period. a dental hy ... | 2001 | 11795450 |
| [metronidazole in the treatment of refractory periodontitis]. | in this article the clinical and microbiological effects of systemic metronidazole in the treatment of refractory periodontitis in adult patients is discussed. for this study, 27 adult patients with detectable bacteroides forsythus and culture negative for actinobacillus actinomycetemcomitans were selected. after renewed supra- and subgingival debridement, patients were treated with systemic metronidazole (500 mg tid) for 7 days. mean probing pocket depth, probing attachment level and bleeding i ... | 2000 | 11383022 |
| the effect of statherin and its shortened analogues on anaerobic bacteria isolated from the oral cavity. | the susceptibility (mic) of 44 strains of anaerobic bacteria isolated from the oral cavity and 3 standard strains to statherin and its c-terminal fragments with sequences qyqqytf, yqqytf, qqytf, qytf and ytf was determined by means of plate dilution technique in brucella agar with 5% content of defibrinated sheep's blood, menadione and hemin. the culture was anaerobic. as shown, at concentrations from 12.5 to 100 microg/ml statherin and its c-terminal fragments inhibited the growth of anaerobic ... | 2000 | 11293657 |
| comparison of microbial composition in the subgingival plaque of adult crowded versus non-crowded dental regions. | it has been reported in the literature that certain species of bacteria (periodontopathogens) present in the subgingival plaque are associated with destructive periodontal disease. the purpose of this study was to investigate and compare the presence and proportional distribution of periodontopathogens in the subgingival plaque of adult crowded versus non-crowded dental regions. thirty adult patients with anterior dental crowding were selected from the orthodontic clinic of the university of pen ... | 2000 | 11307190 |
| significance of detection of porphyromonas gingivalis, bacteroides forsythus and treponema denticola in periodontal pockets. | the relationship between the detection of porphyromonas gingivalis, bacteroides forsythus and treponema denticola in subgingival plaque samples of periodontal pockets and periodontal status was evaluated using the polymerase chain reaction (pcr). a total of 165 sites in 60 periodontitis patients were examined, and the relationships between the detection of each of the three bacterial species and the pocket depth and bleeding on probing (bop) were analyzed. the detection ratios of p. gingivalis, ... | 2000 | 11212582 |
| oral chlamydia trachomatis in patients with established periodontitis. | periodontitis is considered a consequence of a pathogenic microbial infection at the periodontal site and host susceptibility factors. periodontal research supports the association of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and bacteroides forsythus, and periodontitis; however, causality has not been demonstrated. in pursuit of the etiology of periodontitis, we hypothesized that the intracellular bacteria chlamydia trachomatis may play a role. as a ... | 2000 | 11218493 |
| periodontopathic bacteria in children with down syndrome. | it is widely known that individuals with down syndrome (ds) often develop severe early-onset periodontal diseases. in this study, we examined the prevalence of periodontopathic bacteria in ds children to determine if specific pathogens are acquired in their childhood. | 2000 | 10711615 |
| adherence of bacteroides forsythus to host cells. | the adherence characteristics of bacteroides forsythus to host cells, was examined. four laboratory strains and twelve clinical isolates of b. forsythus were used. all strains demonstrated different haemagglutination activities. the haemagglutination of b. forsythus was inhibited strongly by amino acids such as l-arginine, l-histidine, l-lysine and l-alanine. the adherence to polymorphonuclear leucocytes (pmnl) was weak except for b. forsythus atcc 43037 and omz 408. the adherence of these strai ... | 2000 | 10738984 |
| herpesviruses and periodontopathic bacteria in trisomy 21 periodontitis. | little is known about the etiology and pathogenesis of periodontal disease in trisomy 21 patients. this study determined the occurrence of herpesviruses and putative periodontopathic bacteria in trisomy 21 periodontitis. | 2000 | 10776924 |
| profile of subgingival microbiota in children with primary dentition. | eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ... | 2000 | 10791707 |
| the prevalence of pathogenic periodontal microflora in healthy young adult smokers. | smoking is a major risk factor in periodontitis, although the mechanisms of its effects are not well understood. the overall goal of this clinical study was to determine if smoking enhances the colonization of the oral cavity by pathogenic bacteria in a periodontitis-free population. the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsythus, and treponema denti ... | 2000 | 10807119 |
| interactions between periodontal bacteria and human oral epithelial cells: fusobacterium nucleatum adheres to and invades epithelial cells. | bacteria are causative agents of periodontal diseases. interactions between oral bacteria and gingival epithelial cells are essential aspects of periodontal infections. using an in vitro tissue culture model, a selected group of gram-negative anaerobic bacteria frequently associated with periodontal diseases, including bacteroides forsythus, campylobacter curvus, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, and prevotella intermedia, were examined for their ability to ... | 2000 | 10816455 |
| checkerboard dna-dna hybridization analysis of endodontic infections. | the purpose of this investigation was to examine the microbiota of infected root canals by using a molecular genetic method. | 2000 | 10846131 |
| the periodontal abscess (i). clinical and microbiological findings. | little information is available regarding the diagnosis and microbiology of periodontal abscesses. the aim of this descriptive clinical and microbiological study was to provide more information in order to help in the characterisation of the periodontal abscess associated to periodontitis. | 2000 | 10883867 |
| microbial comparison of smoker and non-smoker adult and early-onset periodontitis patients by polymerase chain reaction. | a number of bacterial species are involved in the aetiology of periodontitis and include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus and treponema denticola. several studies have shown differences in the microflora between the various forms of periodontal disease. it is recognised that smoking is a risk factor for periodontal disease, but there are conflicting reports on whether or not smoking has an effect on the periodontal micro ... | 2000 | 10883871 |
| novel apoptosis-inducing activity in bacteroides forsythus: a comparative study with three serotypes of actinobacillus actinomycetemcomitans. | bacteroides forsythus, which has been reported to be associated with periodontitis but has not been recognized as a key pathogen, was found to induce cytolytic activity against hl-60 and other human leukemic cells. this cytolytic activity was demonstrated according to three different criteria: (i) loss of both mitochondrial membrane potential and membrane integrity in cells treated with bacterial extracts and then with rh123 and propidium iodide, respectively, as demonstrated by flow cytometry; ... | 2000 | 10899863 |
| "checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study. | we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status. | 2000 | 10914791 |
| effect of the interleukin-1 genotype on monocyte il-1beta expression in subjects with adult periodontitis. | an association has been reported between polymorphisms in the genes encoding il-1alpha (-889) and il-1beta (+3953) (periodontitis susceptibility trait, pst), and an increased severity of periodontitis (18). the il-1beta polymorphism was reported to correlate with increased il-1beta expression by monocytes in response to bacterial stimulants. in the present study, we determined if pst positive subjects with periodontitis exhibit elevated production of il-1beta, compared to pst negative periodonti ... | 2000 | 10929872 |
| prevalence of bacteroides forsythus, porphyromonas gingivalis and actinobacillus actinomycetemcomitans in subgingival microflora of japanese patients with adult and rapidly progressive periodontitis. | this study investigated the prevalence of bacteroides forsythus, porphyromonas gingivalis and actinobacillus actinomycetemcomitans among various periodontitis patients and healthy individuals in japan, and correlated it with clinical parameters. | 2000 | 10959786 |
| the effect of repeated professional supragingival plaque removal on the composition of the supra- and subgingival microbiota. | the purpose of the present investigation was to determine the effect of weekly professionally administered supragingival plaque removal on the composition of the supra and subgingival microbiota. | 2000 | 10983597 |
| comparison of the microbiota of supra- and subgingival plaque in health and periodontitis. | the purpose of the present investigation was to compare the microbial composition of supra and subgingival plaque in 22 periodontally healthy (mean age 32+/-16 years) and 23 adult periodontitis subjects (mean age 51+/-14 years). | 2000 | 10983598 |
| prevalence of periodontal pathogens in localized and generalized forms of early-onset periodontitis. | the primary objectives of this study were to investigate the prevalence of 8 putative periodontal pathogens in subjects with early-onset periodontitis (eop) and to evaluate the microbial differences between localized and generalized forms of this periodontal disease condition. thirty-one females and 11 males with a mean age of 30.3 (s.d. 4.0) years were examined. seventeen subjects had generalized (geop) and 25 had localized early-onset periodontitis (leop). subgingival plaque samples were assay ... | 2000 | 10983884 |
| detection of major putative periodontopathogens in korean advanced adult periodontitis patients using a nucleic acid-based approach. | although extensive microbial analyses have been performed from subgingival plaque samples of periodontitis patients, systematic analysis of subgingival microbiota has not been carried out in a korean population so far. the purpose of this study was to describe the prevalence of major putative periodontopathogens in korean patients by culture-independent methods. | 2000 | 11022767 |
| microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. | the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years). | 2000 | 11034118 |
| differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands. | the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ... | 2000 | 11037754 |
| identification of periodontal pathogens in atheromatous plaques. | recent studies suggest that chronic infections including those associated with periodontitis increase the risk for coronary vascular disease (cvd) and stroke. we hypothesize that oral microorganisms including periodontal bacterial pathogens enter the blood stream during transient bacteremias where they may play a role in the development and progression of atherosclerosis leading to cvd. | 2000 | 11063387 |
| microbiological parameters associated with il-1 gene polymorphisms in periodontitis patients. | polymorphisms in the cluster of il-1 genes have been significantly associated with the severity of adult periodontitis. the purpose of this study was to compare microbiological parameters in il-1 genotype negative and positive adult subjects with a range of periodontitis severities. | 2000 | 11073323 |
| serum antibody titers to bacteroides forsythus in elderly subjects with gingivitis or periodontitis. | the risks for periodontal disease appear to increase with age. | 2000 | 11073327 |
| photoactive porphyrin derivative with broad-spectrum activity against oral pathogens in vitro. | photodynamic therapy (pdt) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. pdt is a potential adjunct to scaling and root planing in the treatment of periodontal disease. however, the effectiveness of porphyrin derivatives against microorganisms has been limited because some gram-negative bacteria are refractory to photodynamic treatment with these agents. we have designed a porphyrin ... | 2000 | 11083641 |
| localization of major, high molecular weight proteins in bacteroides forsythus. | bacteroides forsythus produces species-specific major proteins with high molecular weights of 270 and 230-kda (270k and 230k). a specific antibody raised against 270k was used for western blot analysis and immunoelectron microscopy. western blot analysis showed that the 270k and 230k proteins were immunologically similar. immunogold labeling of ultrathin-sectioned bacterial cells and biochemical fractionation revealed that these proteins were localized at the outermost cell surface, not in the c ... | 2000 | 11092242 |
| quantitation of bacteroides forsythus in subgingival plaque comparison of immunoassay and quantitative polymerase chain reaction. | our objective was to compare three methods (enzyme-linked immunosorbent assay [elisa], endpoint and quantitative polymerase chain reaction [e-pcr and q-pcr]) for detection and quantitation of bacteroides forsythus in 56 plaque samples from seven subjects with progressive periodontal disease. samples collected in buffer were pelleted and resuspended in 500 microl of water. fifty microl aliquots were removed for an elisa performed on bacteria or plaque immobilized on 96-well plates and probed with ... | 2000 | 10576699 |
| humoral immunity host factors in subjects with failing or successful titanium dental implants. | treatment with titanium dental implants is in general successful. however, an unknown number of implants do not integrate and are removed either by exfoliation or at the time of second stage surgery. it would be of importance to identify subjects at risk and predict early implant failure. | 2000 | 11140553 |
| individual diversities in interferon gamma production by human peripheral blood mononuclear cells stimulated with periodontopathic bacteria. | polarization of type 1 (th1) or type 2 (th2) immune responses determines the prognosis of many infectious diseases. interferon gamma (ifn-gamma) and il-4 are key cytokines for the development of type 1 and type 2 immune responses, respectively. the aim of this study was to examine individual diversities in the polarization of type 1 and type 2 responses against periodontopathic bacteria. peripheral blood mononuclear cells (pbmcs) from adult periodontitis (ap) patients and healthy (h) subjects we ... | 2000 | 11144404 |
| porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets. | recent evidence has established an association between chronic periodontitis and cardiovascular disease. periodontitis is a chronic inflammatory disease caused by a small group of gram-negative bacteria, of which porphyromonas gingivalis is considered an important causative agent. it has been proposed that dental plaque bacteria and their products can disseminate into the bloodstream from the site of infection and promote thromboembolic events associated with atherosclerosis and myocardial infar ... | 2000 | 11154438 |
| profile of subgingival microbiota in children with mixed dentition. | a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ... | 2000 | 11155173 |
| early colonization of dental implants by putative periodontal pathogens in partially edentulous patients. | there is limited scientific information available on the early colonization of the peri-implant pockets in partially edentulous individuals. knowledge about this process is one step in better understanding the etiology and pathogenesis of peri-implantitis. in this study, the early colonization of the peri-implant pockets by putative periodontal pathogens was studied in 20 partially edentulous individuals using anaerobic culture techniques. at baseline, the presence and levels of putative periodo ... | 2000 | 11168244 |
| molecular epidemiology and association of putative pathogens in root canal infection. | the purpose of this study was to investigate the epidemiology of putative pathogens in root canals with apical periodontitis and to determine the associations among the putative pathogens. eighteen symptomatic and 20 asymptomatic teeth from 36 subjects were studied. this research was performed with polymerase chain reaction and hybridization using rrna-based oligonucleotide probes. the most frequently found species was fusobacterium sp. (68.4%), followed by peptostreptococcus micros (44.7%) and ... | 2000 | 11199802 |
| assessment of periradicular microbiota by dna-dna hybridization. | in the present study the "checkerboard" dna-dna hybridization technique was used to identify bacteria in periapical endodontic lesions of asymptomatic teeth. thirty-four patients with root-filled teeth and apical periodontitis were divided into two groups, each containing 17 patients. in group 1, a marginal incision was performed during surgery to expose the lesion, and in group 2, a submarginal incision was applied. the gingiva and mucosa were swabbed with an 0.2% chlorhexidine gluconate soluti ... | 2000 | 11202881 |
| bacteria of asymptomatic periradicular endodontic lesions identified by dna-dna hybridization. | possible inclusion of contaminant bacteria during surgery has been problematic in studies of periradicular lesions of endodontic origin. therefore, in this study, two different surgical techniques were compared. a second problem is that some difficult to cultivate species may not be detected using bacteriological methods. molecular techniques may resolve this problem. dna-dna hybridization technology has the additional advantage that dna is not amplified. the purpose of this investigation was to ... | 2000 | 11202882 |
| comparison of profiles of key periodontal pathogens in periodontium and endodontium. | despite the established anatomical relationship between the periodontal and pulpal tissues, bacterial migration between endodontium and periodontium is still under discussion. the objective of this study was an investigation of profiles of periodontal pathogens in pulpal and periodontal diseases affecting the same tooth by means of 16s rrna gene directed polymerase chain reaction (pcr). 31 intact teeth with both pulp and marginal infections were investigated. the diagnosis was based on clinical ... | 2000 | 11202893 |
| the relationship between place bana reactivity and clinical parameters in subjects with mental disabilities. | the purpose of the present investigation was to determine whether subjects institutionalized with mental retardation have a relationship between periodontal clinical parameters and the presence of the bana-positive periodontal pathogens porphyromonas gingivalls, treponema denticola, and bacteroides forsythus in their subgingival plaques. fifty institutionalized subjects (25 patients with down syndrome and 25 subjects with mental retardation) were matched with respect to age and sex. periodontal ... | 2000 | 11203898 |
| vaccination and periodontitis: myth or reality. | in most industrialised countries approximately 15% of the population has enhanced risk for moderate to severe periodontitis. the disease is caused by infection by gram-negative, anaerobic bacteria including porphyromonas gingivalis and bacteroides forsythus. there is evidence that p. gingivalis is a key pathogen. using ligature-induced periodontitis in the non-human primate macaca fascicularis as a model, we immunised 10 animals using intact killed p. gingivalis and saf-m adjuvant and 10 control ... | 2000 | 12666959 |
| molecular detection of bacteroides forsythus in infected root canals. | the purpose of the present study was to investigate the occurrence of bacteroides forsythus in infections of dental root canals. eleven samples from infected root canals were analyzed by four different molecular methods. the prevalence of the monitored species varied as a function of the detection method. the polymerase chain reaction-dna probe method after immunocapture yielded the highest prevalence value (6/11), whereas the lowest value was observed with the slot-blot (3/11). of the 11 canal ... | 1999 | 10530257 |
| comparing periodontal disease in identical twins: a case report. | previous investigators have shown that numerous environmental and genetic variables may contribute to the pathogenesis of periodontal disease. this case report presents clinical and laboratory findings of a set of caucasian female identical twins. one patient presented clinically with mild gingivitis and no clinical or radiographic signs of periodontitis. the other exhibited gingivitis with localized, moderate-to-severe periodontitis. neither patient reported a history of systemic conditions tha ... | 1999 | 10634119 |
| proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria. | anaerobic culture is employed routinely in the primary isolation of periodontal pathogenic bacteria. however, little or no data exist on the relative abilities of the coy anaerobic chamber (coy laboratory products, grass lake, mich.), the gaspak (becton dickinson microbiology systems, cockeysville, md.), and the anaeropack (mitsubishi gas chemical america, inc., new york, n.y.) systems to grow important periodontal species, including porphyromonas gingivalis, prevotella intermedia/nigrescens, ba ... | 1999 | 9854085 |
| anaerobic periodontal infections as risk factors for medical diseases. | advanced forms of periodontal disease are associated with the overgrowth of a limited number of gram-negative anaerobic species in plaques found in periodontal pockets. double-blind clinical trials of metronidazole and doxycycline, combined with debriding of the tooth surfaces, have significantly reduced the need for periodontal surgery. epidemiologic studies have indicated that untreated periodontal disease could be a risk factor for preterm delivery of low birth weight infants, coronary heart ... | 1999 | 11095764 |
| clinical and microbiological characteristics of smokers with early onset periodontitis. | cigarette smoking is a potential risk factor which has recently been associated with periodontal disease progression. the objective of this study was to compare the microbial profile of smokers and non-smokers in a group of patients with early onset periodontitis. the study population consisted of 60 healthy individuals, 40 males and 20 females aged 22 to 35 yr, exhibiting early onset periodontitis. thirty patients were smokers (30.9 cigarettes/d) and 30 non-smokers. smokers had a higher proport ... | 1999 | 10086883 |
| the microbiota of periodontal pockets with different depths in therapy-resistant periodontitis. | this study presents the composition of the cultivable microbiota colonising periodontal pockets of different depths among 2 patient-groups classified as non-responsive (nr-group; 11 participants) or responsive (r-group; 10 participants) to periodontal treatment. microbiological samples from three types of pocket (< 4 mm deep a-samples; 4-5 mm b-samples; > 5 mm c-samples) were analysed by cultural methods for putative periodontitis pathogens, microbial groups constituting > or = 5% of the total c ... | 1999 | 10100039 |
| microbiota of successful osseointegrated dental implants. | the long-term survival of dental implants depends, in part, on control of bacterial infection in the peri-implant region. periodontal pathogens colonized implants symptomatic through infection, whereas the microbiota of successful implants was similar to that of periodontal health. this study examined the impact on the peri-implant microbiota of crown restorations; implant type; length of time of loading; history of implant or periodontal infections; and whether implants replaced single or multi ... | 1999 | 10102550 |
| simultaneous detection of bacteroides forsythus and prevotella intermedia by 16s rrna gene-directed multiplex pcr. | in a 16s rrna gene-directed multiplex pcr, prevotella intermedia- and bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. a 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. a total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation. | 1999 | 10203541 |
| characterization of bacteroides forsythus strains from cat and dog bite wounds in humans and comparison with monkey and human oral strains. | bacteroides forsythus strains recovered from cat and dog bite wound infections in humans (n = 3), monkey oral strains (n = 3), and the human oral atcc 43037 type strain were characterized by using phenotypic characteristics, enzymatic tests, whole cell fatty acid analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis, pcr fingerprinting, and 16s rdna (genes coding for rrna) sequencing. all three bite wound isolates grew on brucella agar supplemented with 5% sheep ... | 1999 | 10325363 |
| relationship between herpesviruses and adult periodontitis and periodontopathic bacteria. | various mammalian viruses and specific bacteria seem to play important roles in the pathogenesis of human periodontitis. this study examined the relationship between subgingival herpesviruses and periodontal disease and potential periodontopathic bacteria in 140 adults exhibiting either periodontitis or gingivitis. | 1999 | 10368051 |
| longitudinal study of predictive factors for periodontal disease and tooth loss. | longitudinal assessment of risk factors for periodontal disease is necessary to provide evidence that a putative risk factor or risk indicator is a true risk factor. the purpose of the present study was to explore longitudinally a variety of markers as possible periodontal risk factors in subjects with little or no periodontal disease at baseline. 415 subjects with mild or little periodontal disease were examined: medical and dental history; socioeconomic profile, clinical measurements, microbia ... | 1999 | 10382577 |
| effect of initial periodontal therapy on the frequency of detecting bacteroides forsythus, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans. | porphyromonas gingivalis, bacteroides forsythus, and actinobacillus actinomycetemcomitans have been described as periodontopathic bacteria, and their presence in subgingival pockets can lead to development of periodontal disease. until now, clinical parameters have been used to evaluate the effect of conventional periodontal treatment without microbiological parameters. the present study examined the microbiological effects of initial periodontal therapy using dna probes and the polymerase chain ... | 1999 | 10397511 |
| clinical and microbiological effects of initial periodontal therapy in conjunction with amoxicillin and clavulanic acid in patients with adult periodontitis. a randomised double-blind, placebo-controlled study. | the aim of the present study was to investigate the clinical and microbiological effects of initial periodontal therapy in conjunction with systemic amoxicillin plus clavulanic acid in adult periodontitis patients using a double-blind, parallel-group, and placebo-controlled protocol. 21 patients with a clinical diagnosis of generalised adult periodontitis were recruited. clinical measurements and microbiological assessments were carried out at baseline, 3, and 12 months post-treatment. approxima ... | 1999 | 10412851 |
| inability of intact cells of treponema denticola to degrade human serum proteins iga, igg and albumin. | treponema denticola has been shown to be associated with periodontitis in man and animals. the organism ferments amino acids and thrives on the proteins in the periodontal pocket. accordingly, t. denticola possesses various proteolytic enzymes, including a chymotrypsin-like protease, capable of hydrolyzing a whole range of proteins, including immunoglobulins. yet, it is not clear whether the intact cells of t. denticola can degrade immunoglobulins and albumin. the purpose of this study was to cl ... | 1999 | 10412854 |
| porphyromonas gingivalis prevalence related to other micro-organisms in adult refractory periodontitis. | forty-six adult periodontal patients, selected on the basis of clinical examination, and 46 adult healthy subjects were examined. the subgingival plaque samples from one inflammatory and one non-inflammatory site of each periodontal patient were studied to determine porphyromonas gingivalis prevalence related to other periodontal micro-organisms and to periodontal tissue destruction. the results showed porphyromonas gingivalis as the main pathogenic micro-organism isolated in the inflammatory si ... | 1999 | 10423739 |
| periodontitis associated bacteria in supragingival plaque of dental hygienists: stability of carrier state and clinical development. | the purpose of this study was the clinical and microbiological re-examination of dental hygienists, who, 30 months before, had shown remarkably high supragingival levels of periodontitis-associated micro-organisms. interdental plaque was collected from the same molar sites and investigated by the same immunofluorescence assay with taxa-specific monoclonal antibodies as at the initial examination. on average, the 15 re-examined subjects showed slightly increased plaque levels but unchanged bleedi ... | 1999 | 10424388 |
| phylogeny of the defined murine microbiota: altered schaedler flora. | the "altered schaedler flora" (asf) was developed for colonizing germfree rodents with a standardized microbiota. the purpose of this study was to identify each of the eight asf strains by 16s rrna sequence analysis. three strains were previously identified as lactobacillus acidophilus (strain asf 360), lactobacillus salivarius (strain asf 361), and bacteroides distasonis (strain asf 519) based on phenotypic criteria. 16s rrna analysis indicated that each of the strains differed from its presump ... | 1999 | 10427008 |
| tetracycline-coated polytetrafluoroethylene barrier membranes in the treatment of intraosseous periodontal lesions. | periodontal pathogens are detrimental to periodontal healing in barrier membrane-assisted periodontal therapy. tetracycline-coating of barrier membranes may reduce levels of infecting pathogens. this study evaluated the clinical and microbiological effects of tetracycline-coated expanded polytetrafluoroethylene (t-eptfe) barrier membranes in the treatment of 2- to 3-wall intraosseous periodontal lesions around mandibular molars. | 1999 | 10505803 |
| improved multiplex pcr using conserved and species-specific 16s rrna gene primers for simultaneous detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, and porphyromonas gingivalis. | among putative periodontal pathogens, actinobacillus actinomycetemcomitans, bacteroides forsythus, and porphyromonas gingivalis are most convincingly implicated as etiological agents in periodontitis. therefore, techniques for detection of those three species would be of value. we previously published a description of a multiplex pcr that detects a. actinomycetemcomitans and p. gingivalis. the present paper presents an improvement on that technique, which now allows more sensitive detection of a ... | 1999 | 10523542 |
| identification and nucleotide sequence of the heat shock protein 60 (groel) gene of bacteroides forsythus. | bacteroides forsythus is a gram negative anaerobe associated with human periodontal disease. heat shock proteins are immunodominant antigens in bacteria that can elicit strong and protective immune responses. the gene specifying the groel protein (hsp60, heat shock protein 60) of b. forsythus was isolated by pcr amplification using consensus primers based upon published nucleotide sequences of the groel genes of several bacterial species. translation of the gene sequence predicts a protein of 54 ... | 1998 | 10524765 |
| microbiota of health, gingivitis, and initial periodontitis. | this study compared the subgingival microbiota in periodontal health, gingivitis and initial periodontitis using predominant culture and a dna probe, checkerboard hybridization method. 56 healthy adult subjects with minimal periodontal attachment loss were clinically monitored at 3-month intervals for 12 months. more sites demonstrated small increments of attachment loss than attachment gain over the monitoring period. sites, from 17 subjects, showing > or = 1.5 mm periodontal attachment loss du ... | 1998 | 9495607 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| clinical and microbiological features of refractory periodontitis subjects. | the purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showe ... | 1998 | 9495617 |
| rapid chair-side dna probe assay of bacteroides forsythus and porphyromonas gingivalis. | this study compared a rapid, colorimetric dna probe assay designed to be performed in a dental office within 40 min, with anaerobic culture and indirect immunofluorescence microscopy (ifm) for detection of bacteroides forsythus and porphyromonas gingivalis in subgingival plaque samples. the dna probe assay used the periodontal microbial identification test (saigene corporation, bothell, washington, usa). b. forsythus was detected in 46 (52%), 49 (55%) and 39 (44%) of the samples by dna probe, cu ... | 1998 | 9553870 |
| porphyromonas gingivalis, prevotella intermedia and bacteroides forsythus in plaque subjacent to bridge pontics. | this study examined the distribution of p. gingivalis, p. intermedia and b. forsythus in plaque on metallic and porcelain pontics adjacent to healthy and inflamed mucosa. subpontic plaque was collected from 33 inflamed and 31 healthy sites. plaque suspension was incubated with specific rabbit antisera to p. gingivalis (fdc 381), p. intermedia (atcc 25261) and b. forsythus (fdc 335), and the labelled cells disclosed with fluorescein-labelled goat-anti-rabbit igg by indirect immunofluorescence mic ... | 1998 | 9565285 |
| identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using dna-dna hybridization. | the purpose of this investigation was to identify bacterial species present on or in crevicular epithelial cells in healthy and diseased sites using dna probes. in order to achieve this aim, further improvements were made in the separation of unattached bacteria from those adherent to epithelial cells isolated from the human gingival crevice or periodontal pocket. then the dna probes were used to determine the prevalence of detectable dna from 15 microbial species on or in crevicular epithelial ... | 1998 | 9573819 |
| cross-reactivity of specific antibodies directed to heat shock proteins from periodontopathogenic bacteria and of human origin [corrected]. | this study describes the immunological characterization of two different classes of heat shock proteins isolated from periodontopathogenic bacteria. analysis of the n-terminal amino acid sequence of a 74-kda protein from bacteroides forsythus showed a high degree of homology with the dnak protein from escherichia coli. however, this heat shock protein from b. forsythus reacted very weakly with a commercial anti-dnak polyclonal antibody by dot-blotting. groel-like proteins isolated from actinobac ... | 1998 | 9573824 |
| subgingival microbiota in healthy, well-maintained elder and periodontitis subjects. | this investigation compared the site prevalence of 40 subgingival species in 30 periodontally healthy (mean age 36+/-9 years), 35 elders with a well-maintained periodontium (mean age 77+/-5) and 138 adult periodontitis subjects (mean age 46+/-11). subgingival plaque samples were taken from the mesial aspect of each tooth (up to 28 samples) in the 203 subjects at baseline. the presence and levels of 40 subgingival taxa were determined in 5003 plaque samples using whole genomic dna probes and chec ... | 1998 | 9650869 |
| the utility of whole saliva to detect the oral presence of periodontopathic bacteria. | this study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque of 202 subjects. the test bacteria were identified using a 16s rrna-based pcr detection method. each study subject contributed a whole saliva sample and a paper point sample pooled from the deepest periodontal pocket in each quadrant of the dentition. the kappa test revealed a fair agreement between the presence of porphyromonas gingivalis, prevotella intermedia, and treponema denticola in whol ... | 1998 | 9706862 |
| oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis. | the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ... | 1998 | 9722273 |
| potential pathogenic mechanisms of periodontitis associated pregnancy complications. | during normal pregnancy, maternal hormones and locally acting cytokines play a key role in regulating the onset of labor, cervical ripening, uterine contraction, and delivery. maternal infections during pregnancy have been demonstrated to perturb this normal cytokine and hormone-regulated gestation, sometimes resulting in preterm labor, preterm premature rupture of membranes, and preterm low birth weight (plbw), i.e., < 2,500 g and < 37 weeks of gestation. our research focus has been to determin ... | 1998 | 9722707 |
| genetic variations in cytokine expression: a risk factor for severity of adult periodontitis. | periodontitis is a collection of chronic inflammatory diseases that are caused by specific bacteria. the bacteria activate inflammatory mechanisms in the periodontal tissues that destroy collagen and bone that support the teeth. although bacteria are essential for the initiation of periodontitis, the quantity and types of bacteria have not been sufficient to explain the differences in disease severity. in recent years, it has become evident that for many common chronic diseases, there are modify ... | 1998 | 9722717 |