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an ultrastructural and histopathological study of henneguya pellucida n. sp. (myxosporea: myxobolidae) infecting piaractus mesopotamicus (characidae) cultivated in brazil.during a study of myxosporean parasites of cultivated freshwater fish, a new myxosporean species, henneguya pellucida n. sp., was discovered. of the 120 piaractus mesopotamicus sampled, only 10 specimens (8.3%) were infected. yellow, round plasmodia measuring 0.5-3 mm were found in the serous membrane of the visceral cavity and in the tunica externa of the swim bladder. sporogenesis was asynchronous, with the earliest developmental stages aligned prevailingly along the endoplasmic periphery and ...200516218209
adaptation of crops to environment.adaptability is defined as the ability of a crop (or variety) to respond positively to changes in agricultural conditions. the trait is genetically controlled and provides an ability to exploit environmental attributes, both natural and agronomic. values of relative adaptability can be determined by the regression of the yield of the tested crop over the average yield of compared crops from several environments. we evaluated relative adaptability of 12 staple crops in 12 european countries and c ...200516187121
specificity of codling moth (lepidoptera: tortricidae) for the host plant kairomone, ethyl (2e,4z)-2,4-decadienoate: field bioassays with pome fruit volatiles, analogue, and isomeric compounds.codling moth, cydia pomonella (l.), is a severe pest of apples, pears, and walnuts worldwide, and new approaches for precise monitoring and management would be beneficial. ninety-two pome fruit volatiles were formulated in 23 distinct blends, of which a single 4-component blend of 10-carbon esters showed the only significant attraction of moths in field bioassays conducted in both walnut and apple orchards. a single constituent of this blend, ethyl (2e,4z)-2,4-decadienoate--the "pear ester", was ...200515884837
from the anther to the proctodeum: pear (pyrus communis) pollen digestion in osmia cornuta larvae.modifications of the pollen grains of pyrus communis linneaus that occur during the digestion by osmia cornuta (latreille) larvae were studied histochemically. we compared the features of the pollen grains found in the anthers, in the larval cell provisions and in the alimentary canal of the 5th instar larvae. modifications were already evident in the provisions and consisted of protoplast protrusions through the apertures and a decrease in the number of starch-containing pollen grains. after po ...200516105552
evaluation of tolerance to pierce's disease and botrytis in transgenic plants of vitis vinifera l. expressing the pear pgip gene.summary polygalacturonase-inhibiting proteins (pgips) are plant cell-wall proteins that specifically inhibit fungal endo-polygalacturonases (pgs) that contribute to the aggressive decomposition of susceptible plant tissues. the inhibition of fungal pgs by pgips suggests that pgips have a role in plant tolerance to fungal infections and this has been observed in transgenic plants expressing pgips. xylella fastidiosa, the causal agent of pierce's disease (pd) in grapevines, has genes that encode c ...200520565637
cytological and molecular analyses of non-host resistance of arabidopsis thaliana to alternaria alternata.summary when challenged with the necrotrophic fungal pathogen alternaria alternata japanese pear pathotype, all tested ecotypes of arabidopsis plants failed to show hypersensitive cell death, accumulation of detectable levels of reactive oxygen species or accumulation of phytoalexin. we operationally define a. alternata as a non-host pathogen for arabidopsis plants and show that the protection against a. alternata demonstrated in this study is a non-host penetration resistance. to characterize n ...200520565684
gorham's disease or massive osteolysis.gorham's disease is a rare disorder characterized by proliferation of vascular channels that results in destruction and resorption of osseous matrix. since the initial description of the disease by gorham and colleagues (1954) and by gorham and stout (1955), fifty years have elapsed but still the precise etiology of gorham's disease remains poorly understood and largely unknown. there is no evidence of a malignant, neuropathic, or infectious component involved in the causation of this disorder. ...200516012123
interaction of cyclodextrins with aliphatic acetate esters and aroma components of la france pear.the interaction between aliphatic acetate esters, which are the major flavor components of la france pear (pyrus communis l.), and cyclodextrins (cds) was studied to obtain fundamental data for preparing powdered pear flavor materials. the complexing abilities of alpha-, beta-, or gamma-cd with six kinds of aliphatic acetate esters were compared. alpha-cd formed the highest numbers of complexes with all of the esters. all of the cds produced complexes more readily with the more hydrophobic ester ...200515969526
analyses of arbutin and chlorogenic acid, the major phenolic constituents in oriental pear.the hplc retention time, photodiode array uv spectrum analysis, and lc/ms results indicated that arbutin and chlorogenic acid are the main phenolic constituents in oriental pear. the two compounds exist in different organs of the yali pear, which is one of the major cultivars of pyrus bretschnrideri. the contents of arbutin in the leaf bud, floral bud, flower, and young fruit were 11.9, 12.4, 8.29, and 9.92 mg/g fresh weight (fw), respectively. chlorogenic acid amounts in the same organs were 2. ...200515884812
accelerators increase permeability of cuticles for the lipophilic solutes metribuzin and iprovalicarb but not for hydrophilic methyl glucose.effects of diethylsuberate (desu), tributyl phosphate (tbp), and monodisperse ethoxylated alcohols (eas) on rate constants of penetration (k) of model solutes across astomatous cuticular membranes isolated from madagascar ivy (stephanotis floribunda) and pear (pyrus communis) leaves were studied. model solutes (selected on the basis of their octanol/water partition coefficients, k(ow)) were iprovalicarb (log k(ow) = 3.18), metribuzin (log k(ow) = 1.60), and methyl glucose (mg) (log k(ow) = -3.0) ...200515796601
remission induced by interferon alfa in a patient with massive osteolysis and extension of lymph-hemangiomatosis: a severe case of gorham-stout syndrome.the treatment of massive osteolysis with lymphangioma and/or hemangioma (gorham-stout syndrome) has been controversial. the authors report on a patient with multiple massive osteolyses and extensive lymph-hemangiomatosis whose lesions were reduced by interferon alfa therapy. a 2-year-old girl had complained of left chylothorax. thoracoscopy showed an increase in small lymphatic vessels in the chest wall. the chylothorax was improved by coagulation of the lymphatic vessels. later, multiple massiv ...200515793714
total mandibular reconstruction for massive osteolysis of the mandible (gorham-stout syndrome).massive osteolysis (gorham-stout syndrome) is a rare condition of unknown aetiology that is thought to result from a localised endothelial proliferation of lymphatic vessels resulting in destruction and absorption of bone. the diagnosis of gorham-stout syndrome can be made only after first excluding osteolysis from infection, inflammation, endocrine disease, and cancer. the syndrome is rarely seen in the facial skeleton and has a variable prognosis. many treatments have been advocated but only s ...200515749219
gorham disease of the spine: a case report and treatment strategies for this enigmatic bone disease.gorham disease is an extremely rare condition of unknown etiology characterized by progressive osteolysis. only 28 cases of its spinal involvement have been reported, and some of those cases showed kyphosis, kyphoscoliosis, subluxation or dislocation. no definite regimen of treatment has been established yet. a 10-year-old boy presented with a severe and progressive kyphosis over 90 degrees caused by gorham disease from t3 to t12. in situ posterior fusion with a hook and rod system and iliac bon ...200515673977
gorham-stout disease--stabilization during bisphosphonate treatment.a 45-year-old woman presented with recent onset of left-sided chest pain. on clinical examination, these symptoms seemed to be strictly localized to a region that was marked by a long-standing cutaneous erythematous lesion. laboratory results showed no gross abnormalities. radiological imaging including conventional x-ray, mri scans, and 3d ct reconstruction of the rib cage revealed circumscript destruction of the left lateral ribs 9-11. histological analysis of a rib biopsy showed angiomatous h ...200515647829
influence of 1-methylcyclopropene and storage atmosphere on changes in volatile compounds and fruit quality of conference pears.conference pears (pyrus communis l.) were treated with 25 and 50 nl l(-1) 1-methylcyclopropene (1-mcp) at -0.5 degrees c for 24 h, then stored for up to 22 weeks in air (na) and controlled atmosphere (ca). after 7 and 14 weeks of storage, fruits were retreated with 1-mcp. after 7, 14, and 22 weeks of storage, fruits were kept for up to 7 days at 20 degrees c in air for poststorage ripening. the effects of 1-mcp treatment declined with duration of storage in both storage atmospheres, indicating t ...200516332131
[gorham-stout disease: report of a case affecting the right hand with a follow-up of 24 years].gorham-stout disease is a rare idiopathic syndrome with distinctive clinical, pathologic and radiologic features. it is a variant form of osseus angiomatosis associated with massive osteolysis of bone. usually appearing after trauma, the disease is described to occur at any age. especially in case of thoracic involvement (chylothorax), lethal outcomes are reported. in medline, about 200 cases have been described. a patient with osteolysis of the right hand following contusion at the age of two y ...200415368153
characterisation and transferability of apple ssrs to two european pear f1 populations.european pear (pyrus communis l.) is among the important fruit species for which only few genetic studies have been carried out. available evidence indicates that simple sequence repeats (ssr) are very useful as molecular markers because they are codominant, highly polymorphic, abundant and reproducible. the present paper reports more than 100 apple ssr markers in two populations of european pear; a total of 41 ssr markers were then positioned on a genetic linkage map of the cross 'passe crassan ...200415340685
european, chinese and japanese pear fruits exhibit differential softening characteristics during ripening.softening characteristics were investigated in three types of pear fruit, namely, european pear 'la france', chinese pear 'yali', and japanese pear 'nijisseiki'. 'la france' fruit softened dramatically and developed a melting texture during ripening, while 'yali' fruit with and without propylene treatment showed no change in flesh firmness and texture during ripening. non-treated 'nijisseiki' did not show a detectable decrease in flesh firmness, whereas continuous propylene treatment caused a gr ...200415333646
gorham-stout disease of costo-vertebral localization: radiographic, scintigraphic, computed tomography, and magnetic resonance imaging findings.gorham-stout disease is a clinical, radiographic, and histological entity characterized by progressive osteolysis as a result of a histologically benign vascular proliferation involving bone. we present a case involving the cervical and thoracic spine and the osseous thorax, with attention to the clinical, radiographic, scintigraphic, computed tomography, and magnetic resonance imaging findings. these subjects are discussed in the light of the literature.200415323402
close-wedge osteotomy for bony locking stiffness of the elbow in gorham disease patients: a case report.gorham disease is a so-called massive idiopathic osteolysis or vanishing bone disorder. massive osteolysis remains an enigmatic condition that involves various skeletal locations and is caused by endothelial proliferation. the diagnosis is difficult and is established via the association of clinical, radiologic and histologic pictures. treatment modalities yield variable results. we report a case of vanishing bone in the elbow joint and carpal bones following trauma. this 13-year-old boy complai ...200415233238
massive osteolysis (gorham disease) of the maxillofacial skeleton: report of 2 cases. 200414762756
molecular markers derived from rapd, scar, and the conserved 18s rdna sequences for classification and identification in pyrus pyrifolia and p. communis.we generated rapd, scar, and conserved 18s rdna markers for classifying and identifying cultivars of pyrus pyrifolia (japanese pear) and p. communis (european pear). pcr amplification with selected specific primers-lch327up and lch327down-was performed using dna extracted from 25 p. pyrifolia and p. communis cultivars. the 1,380-bp fragment was amplified from p. communis cvs. beurre giffard, cascade, conference, clapp's favorite, packhams triumph, and winter nelis. rapd has only a dominant singl ...200414749847
[the relationship between quality of life of schizophrenic patients and clinical parameters of psychosis].the aim of this study was an analysis of relationship between the quality of life of schizophrenic patients and clinical parameters (positive symptoms, negative symptoms, symptoms of depression, time from start of treatment, total time of all hospitalizations). the research was carried out on 120 in- and out-patients (from 19 to 65 years of age) who fulfilled icd-10 and dsm-iv criteria for schizophrenia. they were in a stable state of improvement during actual treatment. the following instrument ...200415765753
gorhams disease: a literature review and case reports. 20048872763
post-translational modifications of arabinogalactan-peptides of arabidopsis thaliana. endoplasmic reticulum and glycosylphosphatidylinositol-anchor signal cleavage sites and hydroxylation of proline.we have developed a method for separating the deglycosylated protein/peptide backbones of the small arabinogalactan (ag)-peptides from the larger classical arabinogalactan-proteins (agps). agps are an important class of plant proteoglycans implicated in plant growth and development. separation of ag-peptides enabled us to identify eight of 12 ag-peptides from arabidopsis thaliana predicted from genomic sequences. of the remaining four, two have low abundance based on expressed sequence tag datab ...200415322080
attitudes and beliefs among mexican americans about type 2 diabetes.hispanics in the united states have a disproportionately high risk for non-insulin-dependent diabetes mellitus (type 2 diabetes) compared with non-hispanic whites. little is known of the attitudes and beliefs about diabetes in this group. using data from six focus groups of 42 mexican americans (14 men and 28 women), we characterized perceptions about the causes of and treatments for type 2 diabetes. many participants believed diabetes is caused by having a family history of the disease, eating ...200415531816
genetic diversity of phytophthora infestans sensu lato in ecuador provides new insight into the origin of this important plant pathogen.abstract the metapopulation structure of phytophthora infestans sensu lato is genetically diverse in the highlands of ecuador. previous reports documented the diversity associated with four putative clonal lineages of the pathogen collected from various hosts in the genus solanum. this paper simultaneously analyzes diversity of the complete collection of isolates, including a large number that had not yet been reported. this analysis confirmed the existence of three pathogen populations, which a ...200418943538
rainfastness of a microencapsulated sex pheromone formulation for codling moth (lepidoptera: tortricidae).the rainfastness of a microencapsulated sex pheromone formulation for codling moth, cydia pomonella (l.), was evaluated in a series of laboratory experiments with detached apple, pear, and walnut leaves. increasing the intensity and duration of simulated rainfall significantly increased the removal of microcapsules from both the top and bottom of apple leaves. the removal of microcapsules was significantly higher from the top versus the bottom of leaves at all rates tested. leaf angle was a sign ...200415666755
seasonal susceptibility of 'bartlett' pears to codling moth (lepidoptera: tortricidae) infestation and notes on diapause induction.seasonal susceptibility of 'bartlett' pear, pyrus communis l., to codling moth, cydia pomonella (l.), infestation, successful completion of larval development after infestation, and the induction of c. pomonella diapause was studied from 1992 through 1995. the seasonal variation in c. pomonella infestation and larval survival were effected by changes in fruit maturity. in late may through mid-june, pears were hard and were not as successfully infested by c. pomonella and produced less larvae com ...200415279281
fluorescence shell: a novel view of sclereid morphology with the confocal laser scanning microscope.confocal laser scanning microscopy (clsm) was used to observe sclereids from stems of avicennia germinans and from fruits of two species of pear (pyrus calleryana "bradford" and p. communis "red bartlett"). the images obtained from thick (25 to 100 microm) free-hand sections were, in certain respects, far superior to those obtained by other, more invasive and time-consuming microscopic techniques upon which previous reports of sclereid morphology were based. the cell wall surfaces, including the ...200415170758
free amino acid composition of quince (cydonia oblonga miller) fruit (pulp and peel) and jam.twenty-one free amino acids present in several samples of quince fruit (pulp and peel) and quince jam (homemade and industrially manufactured) were analyzed by gc/fid. the analyses showed some differences between quince pulps and peels. generally, the highest content in total free amino acids and in glycine was found in peels. as a general rule, the three major free amino acids detected in pulps were aspartic acid, asparagine, and hydroxyproline. for quince peels, usually, the three most abundan ...200414995121
irradiation disinfestation of apple maggot (diptera: tephritidae) in hypoxic and low-temperature storage.apple maggot, rhagoletis pomonella (walsh), is a quarantine pest of apples, malus domestica borkhausen, and pears, pyrus communis l., shipped from much of the united states and canada. as such, these fruits shipped from infested areas to uninfested areas must undergo a quarantine disinfestation treatment. the objective of this research was to develop irradiation quarantine treatments against apple maggot considering that fruit hosts may be stored under hypoxic or cold conditions when they are ir ...200415384333
what do herbalists suggest to diabetic patients in order to improve glycemic control? evaluation of scientific evidence and potential risks.in the course of 12 continuing education seminars given in different regions of italy in 2001, we distributed a questionnaire to all the attending herbalists asking information about the herbal remedy and dietary supplement they mainly recommended to subjects who required a "natural" treatment to control glycemia. we distributed 720 questionnaires and we received 685 completed ones. we have compiled a short review on the efficacy and safety of the 10 most frequently advised products for each cat ...200415666575
genetic characterization of erwinia amylovora strains by amplified fragment length polymorphism.erwinia amylovora is one of the most important pathogens of pear and apple and is subject to strict quarantine regulations worldwide, although its patterns of dispersal are largely unknown. previous attempts to fingerprint e. amylovora strains by molecular techniques have detected very little polymorphism because of the high genetic homogeneity of this bacterium. our aim was to establish and test a typing method to quantify genetic diversity among strains of this plant pathogen.200414723691
some coccidial parasites of the lizard amphisbaena alba (reptilia: amphisbaenia: amphisbaenidae).five parasites are described in the lizard amphisbaena alba (amphisbaenidae) from the state of par , north brazil. mature oocysts of choleoeimeria amphisbaenae n. sp., are passed already mature in the faeces. they are ellipsoidal-cylindrical, average 33.7 x 22.8 m and are devoid of micropyle, oocyst residuum or polar body. the colourless wall is smooth and of 2 layers. the 4 dizoic sporocysts have no stieda body and average 13 x 9.3 m. endogenous stages develop in the epithelial cells of the g ...200414762520
the plant pathogen erwinia amylovora produces acyl-homoserine lactone signal molecules in vitro and in planta.we report for the first time the production of acyl homoserine lactones (ahls) by erwina amylovora, an important quarantine bacterial pathogen that causes fire blight in plants. e. amylovora produces one n-acyl homoserine lactone [a n-(3-oxo-hexanoyl)-homoserine lactone or a n-(3-hydroxy-hexanoyl)-homoserine lactone] quorum sensing signal molecule both in vitro and in planta (pear plant). given the involvement of ahls in plant pathogenesis, we speculate that ahl-dependent quorum sensing could pl ...200415598530
[osmolality of frequently consumed beverages].the objective of this work was to determine the osmolality of beverages frequently consumed by children and adolescents due to the scarce information available in our country. the samples were grouped as follows: milks; refreshments; beverages based on fruits, vegetables, cereals, and tubers; sport drinks; energizing drinks; oral rehydrating solutions; reconstituted drinks and infusions. a vapor pressure digital osmometer was used, five samples of each beverage from different lots were analyzed. ...200415602899
[relationship between breaking of dormancy and reactive oxygen species metabolism in flower buds of pear].the metabolism of reactive oxygen species in pear (pyrus bretschneideri rehd.) flower buds changes greatly during their natural dormancy in winter. the o(-.)(2) production rate increases rapidly during the period of dormancy, but decreases when dormancy finishes (fig. 5). h(2)o(2) content goes up significantly at the early stage of dormancy, but afterwards falls gradually (fig. 5). however, ascorbic acid (asa) and reduced glutathione (gsh) contents show a different changing trend: descending at ...200415643086
alpha-gustducin immunoreactivity in the airways.the g-protein subunit alpha-gustducin is a marker of chemoreceptive cells. in the present study, we examined the immunohistochemical localization of alpha-gustducin in rat airway epithelium both by light and electron microscopy. alpha-gustducin immunoreactivity was found in solitary cells that presented ultrastructural features of chemoreceptor cells, i.e. flask-shaped or pear-shaped, with an apical process with thin microvilli protruding into the lumen. the immunostaining was mainly concentrate ...200415654652
identification of caveolae and their signature proteins caveolin 1 and 2 in the lens.this study shows that caveolae are present in lens epithelia of rabbit and guinea pig under normal conditions. caveolae are unique lipid membrane microdomains observed in many cell types. they are believed to play crucial roles in a variety of basic physiological functions including signal transduction, lipid and transcellular transport. using tem, immunocytochemistry and immunoblotting we show for the first time the existence of caveolae and the co-localization of their signature marker integra ...200415381033
effects of culture media and environmental factors on mycelial growth and pycnidial production of potebniamyces pyri.potebniamyces pyri (anamorph phacidiopycnis piri) is the causal agent of phacidiopycnis rot of apples and pears. the disease has recently been recognized in pears in the usa. little information on the basic biology of the fungus is available. in this paper, we report the effects of culture media, temperature, water potential and ph on mycelial growth, and the effects of media and light on pycnidia production. prune juice agar was the best for rapid mycelial growth. pear juice agar, apple juice a ...200415449598
response of organic and inorganic carbon and nitrogen to long-term grazing of the shortgrass steppe.we investigated the influence of long-term (56 years) grazing on organic and inorganic carbon (c) and nitrogen (n) contents of the plant-soil system (to 90 cm depth) in shortgrass steppe of northeastern colorado. grazing treatments included continuous season-long (may-october) grazing by yearling heifers at heavy (60-75% utilization) and light (20-35% utilization) stocking rates, and nongrazed exclosures. the heavy stocking rate resulted in a plant community that was dominated (75% of biomass pr ...200415453402
cytoarchitecture of the normal rat olfactory epithelium: light and scanning electron microscopic studies.the three-dimensional cytoarchitecture of the normal rat olfactory epithelium was examined by scanning electron microscopy (sem) of koh digested tissues as well as by light and transmission electron microscopy of plastic sections. observations specimens from the lateral side of the olfactory epithelium allowed identification of four cell types by their surface structure: olfactory neurons, supporting cells, basal cells, and duct cells of the bowman's gland. the olfactory neurons were characteriz ...200415468955
the spinal trigeminal nucleus--considerations on the structure of the nucleus caudalis.the caudal part (nucleus caudalis) of the spinal trigeminal nucleus is considered to be the site of the second order neurons of the nociceptive pathways of the face. recent studies have supported the co-participation in these circuits of the oral part of the same nucleus (nucleus oralis). the aims of the present study are: 1) to determine the morphology of the nucleus caudalis in human preparates; 2) to consider whether there is any structural basis for the pathways of signal transmission observ ...200415478109
nucleotide sequences of a korean isolate of apple stem grooving virus associated with black necrotic leaf spot disease on pear (pyrus pyrifolia).pear black necrotic leaf spot (pbnls) is a disease of pears caused by capillovirus-like particles, which can be observed under the electron microscope. the disease was analyzed by western blot analysis with antisera raised against apple stem grooving virus (asgv) coat protein. cdnas covering the entire genome were synthesized by rt-pcr and race using rna isolated from chenopodium quinoa infected with sap extracted from pear leaves carrying black necrotic spot disease. the complete genome sequenc ...200415528995
correction of facial deformity using a red iii device in a patient with antley-bixler syndrome.antley-bixler syndrome was first described in 1975 and to date at least 44 cases have been reported. in addition to brachycephaly, this syndrome is associated with midface hypoplasia, bilateral radiohumeral synostosis, multiple joint contractures, long bone fractures, dysplastic ears, "pear shaped" nose, and occasionally urogenital or cardiac defects. the authors report a case in a 23-year-old man with severe midface deficiency treated using distraction osteogenesis. a modified le fort iii osteo ...200415547402
development of an enzyme-linked immunosorbent assay for the detection of the fungicide fenarimol.to develop an enzyme-linked immunosorbent assay for the fungicide fenarimol, two synthesized haptens, haptens-1 and -2, and the purchased 4,4'-dda were conjugated to carrier proteins (bsa, klh, and ova). polyclonal antibodies raised against hapten-1,2-klh conjugates in rabbits and the coating antigens of hapten-1,2-bsa conjugates, hapten-2-ova conjugate, and 4,4'-dda-bsa conjugate were screened and selected for the homologous and/or heterologous elisa formats. two competitive indirect elisas wer ...200415563196
evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates.the usefulness of various suggested species demarcation criteria was compared in attempts to determine the taxonomic status of ten new tombusvirus isolates. five of them (lim 1, 2, 3, 5 and 6) were obtained from different sources of commercially grown statice (limonium sinuatum), two (gyp 1 and 2) from different sources of commercially grown gypsophila paniculata and three from water samples, i.e. from a small river (schunter) in northern germany, from a brook (near dossenheim) in southern germa ...200415593416
the phytoalexin-inducible multidrug efflux pump acrab contributes to virulence in the fire blight pathogen, erwinia amylovora.the enterobacterium erwinia amylovora causes fire blight on members of the family rosaceae, with economic importance on apple and pear. during pathogenesis, the bacterium is exposed to a variety of plant-borne antimicrobial compounds. in plants of rosaceae, many constitutively synthesized isoflavonoids affecting microorganisms were identified. bacterial multidrug efflux transporters which mediate resistance toward structurally unrelated compounds might confer tolerance to these phytoalexins. to ...200414714867
disappearing calvarium in gorham disease: mr imaging characteristics with pathologic correlation.gorham disease is a rare condition characterized by intraosseous neoplastic proliferation of hemangiomatous tissue with progressive, massive osteolysis. we present a pathologically proved case of gorham disease that involved the left parietal bone in a 23-year-old man. imaging studies including conventional radiography of the skull, ct, mr imaging, and technetium-99 m (tc-99 m) scintigraphy demonstrated a large skull defect without associated soft tissue mass over the left parietal skull. contra ...200415037464
molecular differentiation of erwinia amylovora strains from north america and of two asian pear pathogens by analyses of pfge patterns and hrpn genes.in order to determine a possible genomic divergence of erwinia amylovora'fruit tree' and raspberry strains from north america, several isolates were differentiated by pulsed-field gel electrophoresis (pfge) analysis, the size of short dna sequence repeats (ssrs) and the nucleotide and deduced amino acid sequences of their hrpn genes. by pfge analysis european strains are highly related, whereas strains from north america were diverse and were further distinguished by the ssr numbers from plasmid ...200415049921
dissection of the host range of the fungal plant pathogen alternaria alternata by modification of secondary metabolism.the filamentous fungus alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. the strawberry pathotype produces host-specific af-toxin and causes alternaria black spot of strawberry. this pathotype is also pathogenic to japanese pear cultivars susceptible to the japanese pear pathotype that produces ak-toxin. the strawberry pathotype produces two related molecular species, af-toxins i and ii: toxi ...200415066029
polygalacturonase and ethanol production in kluyveromyces marxianus: potential use of polygalacturonase in foodstuffs.the coproduction of ethanol and polygalacturonase (pg) in a pilot-scale batch fermentor using yeast extract--glucose (yd)--and sugar beet molasses (sbm)-based media was implemented utilizing a new high-pg-producing strain of kluyveromyces marxianus. a certain growth inhibition was observed in sbm medium, causing ethanol and pg production to be lower. ethanol productivity and accumulation values of 1.94 g/(l x h) and 40 g/l, respectively, were attained in yd, whereas the best fermentation efficie ...200415126703
[effects of zinc and boron nutrition on balsam pear (momordica charantia) yield and quality, and polyamines, hormone, and senescence of its leaves].field trial and physiological determination on the effects of zinc and boron nutrition on balsam pear (momordica charantia) yield and quality and on polyamines, hormone, and senescence of its leaves showed that application of znso4.7h2o and na2b4o7.10h2o on zn and b deficient soils could increase balsam pear yield and its contents of protein, vc and 17 sorts of amino acids, especially the contents of essential amino acids for human body, but decrease the no3(-)-n content. the reason was that zn ...200415139192
ethical issues in the neonatal intensive care unit.the case of baby y presented a difficult and complex ethical dilemma for the family and the staff involved. the issues of religious beliefs and law, up-holding these beliefs in the center of a religious community, financial concerns, and health care workers disagreeing about carrying out treatments made this case one that few will forget. when asked after baby y died how they felt, many members of the staff answered that it should not have gone on as long as it did and that they learned a lot fr ...200415145372
self-reported food hypersensitivity in sweden, denmark, estonia, lithuania, and russia.the main aim of the study was to describe the differences between some northern countries regarding what foods, according to the patients, elicit hypersensitivity symptoms.200415160445
effect of the epicuticular waxes of fruits and vegetables on the photodegradation of rotenone.the effect of epicuticular waxes extracted from fruits (apple, nectarine, pear, and plum) and vegetables (tomato and eggplant) on the photodegradation of rotenone was studied. the waxes affected the decay rate and the degradation pathway of this botanical insecticide. tomato, nectarine, and plum waxes decreased the photodegradation rate compared to controls, whereas apple and pear waxes increased it. rotenone irradiated under sunlight without waxes gave seven photoproducts; in contrast, in the p ...200415161214
sodium bicarbonate enhances biocontrol efficacy of yeasts on fungal spoilage of pears.the addition of 2% (w/v) sodium bicarbonate (sbc) in the suspensions of antagonistic yeast cryptococcus laurentii or trichosporon pullulans significantly limited spore germination and germ tube elongation of penicillium expansum and alternaria alternata in potato dextrose broth (pdb) medium. biocontrol activity of c. laurentii or t. pullulans against postharvest decay caused by p. expansum and a. alternata in pear fruits was significantly increased when c. laurentii or t. pullulans combined with ...200415163586
modulation of intracellular calcium concentrations and t cell activation by prickly pear polyphenols.opuntia ficus indica (prickly pear) polyphenolic compounds (ofpc) triggered an increase in [ca2+]i in human jurkat t-cell lines. furthermore, ofpc-induced rise in [ca2+]i was significantly curtailed in calcium-free buffer (0% ca2+) as compared to that in 100% ca2+ medium. preincubation of cells with tyrphostin a9, an inhibitor of ca2+ release-activated ca2+ (crac) channels, significantly diminished the ofpc-induced sustained response on the increases in [ca2+]i. lanthanum and nifedipine, the res ...200415228091
asian pear pectin administration during presensitization inhibits allergic response to ovalbumin in balb/c mice.a type of respiratory disorder resembling some aspects of human allergic asthma can be induced in mice using ovalbumin. the factors that influence the etiology of asthma are poorly understood even though cytokines are known to play a pivotal role. the purpose of this study was to test the hypothesis whether an administration of asian pear pectin during presensitization could suppress allergic response to ovalbumin in balb/c mice.200415253858
'candidatus phytoplasma mali', 'candidatus phytoplasma pyri' and 'candidatus phytoplasma prunorum', the causal agents of apple proliferation, pear decline and european stone fruit yellows, respectively.apple proliferation (ap), pear decline (pd) and european stone fruit yellows (esfy) are among the most economically important plant diseases that are caused by phytoplasmas. phylogenetic analyses revealed that the 16s rdna sequences of strains of each of these pathogens were identical or nearly identical. differences between the three phytoplasmas ranged from 1.0 to 1.5% of nucleotide positions and were thus below the recommended threshold of 2.5% for assigning species rank to phytoplasmas under ...200415280295
'candidatus phytoplasma', a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects.the trivial name 'phytoplasma' has been adopted to collectively name wall-less, non-helical prokaryotes that colonize plant phloem and insects, which were formerly known as mycoplasma-like organisms. although phytoplasmas have not yet been cultivated in vitro, phylogenetic analyses based on various conserved genes have shown that they represent a distinct, monophyletic clade within the class mollicutes. it is proposed here to accommodate phytoplasmas within the novel genus 'candidatus (ca.) phyt ...200415280299
algr functions in algc expression and virulence in pseudomonas syringae pv. syringae.pseudomonas syringae pv. syringae strain ff5 is a phytopathogen associated with a rapid dieback on ornamental pear trees. p. syringae and the human pathogen pseudomonas aeruginosa produce the exopolysaccharide alginate, a copolymer of mannuronic and guluronic acid. in p. aeruginosa, the response regulator algr (algr1) is required for transcription of algc and algd, which encode key enzymes in the alginate biosynthetic pathway. in p. syringae ff5, however, algr is not required for the activation ...200415289569
development of a one tube-one step rt-pcr protocol for the detection of seven viroids in four genera: apscaviroid, hostuviroid, pelamoviroid and pospiviroid.a one tube-one step rt-pcr was developed for the detection of seven viroids (apple scar skin viroid, apple dimple fruit viroid, pear blister canker viroid, hop stunt viroid, chrysanthemum stunt viroid, citrus exocortis viroid and peach latent mosaic viroid) in four genera that infect eight plant species. the efficiency and specificity of this method were optimized by the use of moloney-murine leukemia virus (m-mlv) reverse-transcriptase and hotstartaq dna polymerase which allowed increase sensit ...200415350729
determination of key odorant compounds in freshly distilled cognac using gc-o, gc-ms, and sensory evaluation.this aim of this work was to identify the odorant compounds responsible for the typical sensory descriptors attributed to freshly distilled cognac spirits, not matured in barrels. panelists were first selected and trained for gas chromatography-olfactometry. among the 150 volatile compounds identified by gas chromatography-mass spectrometry analysis, only 34 are mainly responsible for the odors detected in the spirits. the "butter" descriptor is explained by the presence of diacetyl, the "hay" d ...200415373408
dead flower buds of pear: effect of tree growth control, and alternaria alternata as causal agent.dead (dormant) flower buds of pear is an important phenomenon in pear production in the netherlands. vigourous or unbalanced tree growth and pseudomonas syringae pv. syringae are mentioned as likely causes of dead flower buds. several tree growth control treatments including ethephon. regalis (prohexadione-ca) and root pruning were evaluated. regalis increased disease incidence. the plant stimulant (foliar fertilizer) resistim (potassium phosphonate) reduced disease incidence. pseudomonas syring ...200415756820
characterization of pseudomonas pathovars isolated from rosaceous fruit trees in east algeria.a survey of bacterial diseases due to pseudomonas on rosaceous fruit trees was conducted. in forty two orchards located in the constantine region ( east algeria). pseudomonas isolates were identified on the bases of their cultural and biochemical characteristics . a total of fifty nine phytopathogenic bacteria were isolated from diseased pome and stone fruit trees. thirty one strains comparable to pseudomonas syringae pv. syringae were isolated from cherry (prunus avium l.), plum (p. domestica l ...200415756824
species composition of leaf beetle assemblages in the canopies of apple and pear orchards in hungary and great britain (coleoptera: chrysomelidae).the species richness and species composition of coleoptera assemblages were investigated in the canopies of apple and pear orchards in hungary and in the apple orchards in great britain. the investigations were carried out in hungary (nagykovácsi: 3 plots, kecskemét: 5 plots, sárospatak: 4 plots) between 1990-94, and in great britain in kent (east malling, marden and robertsbridge) in 2001 and 2002. former investigations in hungary revealed that the diversity of coleoptera assemblages in the can ...200415759432
an extracellular protease of pseudomonas fluorescens inactivates antibiotics of pantoea agglomerans.abstract pseudomonas fluorescens a506 and pantoea agglomerans strains eh252 and c9-1 are biological control agents that suppress fire blight, an important disease of pear and apple caused by the bacterium erwinia amylovora. pseudomonas fluorescens strain a506 suppresses disease largely through competitive exclusion of e. amylovora on surfaces of blossoms, the primary infection court, whereas pantoea agglomerans strains eh252 and c9-1 produce antibiotics that are toxic to e. amylovora. in this st ...200418944458
acibenzolar-s-methyl-induced resistance to japanese pear scab is associated with potentiation of multiple defense responses.abstract this study reports the mode of action of acibenzolar-s-methyl (asm) against japanese pear scab, caused by venturia nashicola. pretreatment of potted japanese pear trees with asm reduced scab symptoms and potentiated several lines of plant defense response. this included transcripts encoding polygalacturonase-inhibiting protein (pgip) that were highly and transiently promoted after scab inoculation of plants pretreated with asm, suggesting a possible role for defenses involved in direct ...200418943485
temperature and pomaceous flower age related to colonization by erwinia amylovora and antagonists.abstract fire blight of apple and pear is initiated by epiphytic populations of erwinia amylovora on flower stigmas. predicting this disease and managing it with microbial antagonists depends on an understanding of bacterial colonization on stigmas. detached 'manchurian' crab apple flowers were inoculated with e. amylovora and subjected to a range of constant temperatures or various fluctuating temperature regimes. results may have application to disease risk assessment systems such as the couga ...200418943112
bioavailability of iron to pseudomonas fluorescens strain a506 on flowers of pear and apple.abstract the addition of 0.1 mm fecl(3) to a defined culture medium induces the bacterial epiphyte pseudomonas fluorescens strain a506 (a506) to produce an antibiotic toxic to the fire blight pathogen, erwinia amylovora. consequently, because a506 is registered and applied as a commercial product to suppress e. amylovora before floral infection of pear and apple, the relative availability of iron to a506 on surfaces of pear and apple flowers is of potential significance. an 'iron biosensor' cons ...200418943698
dual activity of a viral lysozyme with high efficiency for growth inhibition of erwinia amylovora.abstract the lysozyme from erwinia amylovora phage phiea1h was investigated for its ability to inhibit growth of bacteria and compared with the lysozyme from escherichia coli phage t4. the assays to measure lysozyme activity included cell lysis and growth inhibition of bacteria. bacterial strains with kanamycin resistance were not affected by lysates containing the phiea1h-enzyme. the titer of micrococcus luteus but not of erwinia amylovora was diminished by cell extracts containing t4 lysozyme. ...200418943701
testing a rapid diagnostic medium for erwinia amylovora and development of a procedure for sampling blossoms in pear orchards.abstract the coliform agar produced by merck was tested for rapid diagnosis of erwinia amylovora (the causal agent of fire blight) in pear blossoms. the medium enabled the diagnosis to be completed within 36 h. diagnoses performed with the medium were confirmed by the biolog and the fatty-acid profile methods. the diagnostic medium was used to determine the spatial distribution of colonized blossoms in the orchards and it was found that e. amylovora may be distributed both in clusters and at ran ...200318943859
genetic analysis of a pathogenic erwinia sp. isolated from pear in japan.abstract four erwinia strains, originally isolated in japan from pear trees with bacterial shoot blight symptoms, were analyzed to determine their genetic relationship with erwinia amylovora and e. pyrifoliae. when genomes were characterized with amplified fragment length polymorphism markers and by comparative groel sequence analysis, the japanese erwinia sp. and south korean e. pyrifoliae strains were placed in the same group, which was phylogenetically distinct from a group of 15 strains of e ...200318944067
temporal dynamics of the biocontrol agent pseudomonas fluorescens strain a506 in flowers in inoculated pear trees.abstract the colonization of individual flowers in mature pear orchards by pseudomonas fluorescens strain a506 applied at different times during bloom was measured to determine the receptivity of flowers to colonization and the extent of intra-tree movement over time. strain a506 populations in flowers open at inoculation were initially about 10(4) cells per flower and increased to approximately 10(6) cells per flower in flowers that were inoculated within about 5 days of opening. however, event ...200318943060
isolation and characterisation of resistant-to-fermentation carbohydrate polymers from cultures of rhizopus nigricans grown on agrofood waste materials.rhizopus nigricans was cultivated in a liquid medium using lemon, mandarin, orange, pear and melon peel or artichoke bracts as the carbon source. in all cultures, a carbohydrate polymer fraction remained resistant to fermentation. these fractions were isolated in gram amounts and characterised. the molecular weight distribution of the fractions and its sugar composition resembles those of the hairy-regions of the pectins. in the fractions, four main carbohydrates were found: 4-7 mol% rha, 42-59 ...200314719820
determination of antioxidant properties of aromatic herbs, olives and fresh fruit using an enzymatic sensor.the aim was to experimentally evaluate the antioxidant capacity of different fresh aromatic herbs (field balm, marjoram, parsley, rosemary, sage, sweet basil), several varieties of olives from central italy ('carboncello', 'rosciolo', 'olivastro', 'coratello', 'leccino', 'frantoio') and several types of fresh fruit (apple, apricot, banana, cherry, fig, grape, medlar, melon, peach, pear, pineapple, plum, water melon, yellow plum) using a superoxide dismutase (sod) biosensor developed by the prese ...200312733012
climatic factors governing plant phenological phases along a norwegian fjord.in the present project, the time of leaf budding and flowering, and partly also of fruit ripening, was studied over 3 years in different cultivated and native plants on a gradient along a western norwegian fjord about 300 km long, from oceanic to relatively continental regions. in the plants investigated, flowering of the red currant was most strongly favoured by oceanic conditions in the outermost part of the fjord. on the other hand, flowering of the apple was earliest in the middle district, ...200312750970
the neuronal structure of the red nucleus in newborn guinea pigs.the preparations, stained according to the nissl and klüver-barrera methods, were used to describe the topography and morphology of the red nucleus (rn) as well as the structure of the rubral perikarya in newborn (p0) guinea pigs. the golgi impregnated preparations were used to distinguish types of neurons. rn is a uniform cell group and has the length from 740 to 860 microm. the nissl stained perikarya were classified into three categories: big, medium-sized and small (a, b, c, respectively). t ...200312769171
identification of odors from overripe mango that attract vinegar flies, drosophila melanogaster.bioassays with a variety of overripe fruits, including mango, plum, pear, and grape, and their extracts showed that odors from overripe mango were most attractive to adult vinegar flies, drosophila melanogaster. combined gas chromatography-electroantennographic detection (gc-ead) analyses of solid-phase microextraction (spme) and tenax extracts of overripe mango odors showed that several volatile compounds, including ethanol, acetic acid, amyl acetate, 2-phenylethanol, and phenylethyl acetate el ...200312775150
structure of the cryptosporidium parvum microneme: a metabolically and osmotically labile apicomplexan organelle.from an em study of thin sections, the rod-like microneme organelles within conventionally glutaraldehyde fixed cryptosporidium parvum sporozoites have been shown to undergo a shape change to a more spherical structure when the sporozoites age in vitro for a period of approximately 12 to 24 h. this correlates with the shape change of intact sporozoites, from motile hence viable thin banana-shaped cells to swollen pear-shaped cells, shown by differential interference contrast light microscopy of ...200312801539
characterization of the first korean isolate of a chlamydia pneumoniae strain.chlamydia pneumoniae is a common pathogen that causes upper and lower respiratory tract infections and is difficult to isolate from clinical specimens. recently, we succeeded in isolating the first c. pneumoniae strain in korea. this study characterizes the morphology, infectivity, and drug sensitivity of the korean strain, designated lkk-1. electron microscopy was performed for thin sections, and the infectivity over time was tested by counting the inclusion-forming units every 12 h. the minimu ...200312824688
the erwinia chrysanthemi phop-phoq operon plays an important role in growth at low ph, virulence and bacterial survival in plant tissue.we have studied the role of acidic ph as a barrier for the colonization of the plant apoplast by erwinia chrysanthemi. a minitransposon containing a promoterless reporter gene, gus, was used for random mutagenesis of the bacterial genome. an acid-sensitive mutant, named bt119, was isolated and had the following differential features with respect to the wild-type strain: (i) inability to grow at ph </= 5.5; (ii) decreased survival at acid ph and in plant tissues; (iii) increased susceptibility to ...200312828634
structure-function relationships affecting the insecticidal and miticidal activity of sugar esters.synthetic sugar esters are a relatively new class of insecticidal compounds that are produced by reacting sugars with fatty acids. the objective of this research was to determine how systematic alterations in sugar or fatty acid components of sugar ester compounds influenced their insecticidal properties. sucrose octanoate, sorbitol octanoate, sorbitol decanoate, sorbitol caproate, xylitol octanoate, xylitol decanoate and xylitol dodecanoate were synthesized and evaluated against a range of arth ...200312852599
chemical characterization and biological effects of sicilian opuntia ficus indica (l.) mill. fruit juice: antioxidant and antiulcerogenic activity.the juice of whole fruits of sicilian cultivars of prickly pear (opuntia ficus indica (l.) mill.) was investigated, and the contents of ascorbic acid, total polyphenols, and flavonoids were determined. in the juice, ferulic acid was the chief derivative of hydroxycinnamic acid and the mean concentration of total phenolic compounds was 746 microg/ml. the flavonoid fraction, analyzed by high-performance liquid chromatography-diode array detection, consisted of rutin and isorhamnetin derivatives. t ...200312903943
apple and pear peel and pulp and their influence on plasma lipids and antioxidant potentials in rats fed cholesterol-containing diets.the aim of this study was to assess the bioactive compounds of apple and pear peel and pulp in vitro and their influence on plasma lipids and antioxidant potentials in vivo. the antioxidant potentials measured by 1,1-diphenyl-2-picrylhydrazyl (dpph), beta-carotene bleaching (beta-carotene), and nitric oxide inhibition radical scavenging (no) tests in apple peel and pulp were significantly higher than in pear peel and pulp, respectively. the ethanol extract of apple peels showed the strongest inh ...200312952433
gorham disease of the cervical spine-a case report and review of the literature.study design a case report and literature review of gorham disease involving the cervical spine.200314501935
light and electron microscopic studies of myxobolus stomum n. sp. (myxosporea: myxobolidae) infecting the blackspotted grunt plectorhynicus gaterinus (forsskal, 1775) in the red sea, egypt.a new myxosporean parasite, myxobolus stomum n. sp., is described from the oral cavity and lips of the blackspotted grunt plectorhynicus gaterinus (forsskal, 1775) in the red sea, egypt. the parasite was observed as tiny aggregates of whitish cysts hardly noticed within the muscles of the oral cavity, especially within the lips. the spores were subspherical and measured 8.5x6.5 microm. polar capsules were equal, pear-shaped, occupied about half of the spore length and measured 4.4x2.4 microm. hi ...200314505043
the influence of antibiotic production and pre-emptive colonization on the population dynamics of pantoea agglomerans (erwinia herbicola) eh1087 and erwinia amylovora in planta.stigma colonization by erwinia amylovora is the crucial first step in the development of most fire blight infections in apple and pear trees. suppression at this point of the disease process by antagonists of e. amylovora, such as pantoea agglomerans (erwinia herbicola) strain eh1087, is a rational approach to control fire blight. we tested the hypothesis that the ability of e. amylovora to compete with eh1087 for colonization of a stigma is reduced by the potential for eh1087 to produce the phe ...200314510856
chemical and biochemical changes in prickly pears with different ripening behaviour.chemical and biochemical changes were studied in ripening prickly pears from three opuntia morphospecies with different ripening behaviour: naranjona (o. ficus-indica), blanca cristalina (opuntia sp.), and charola (o. streptacantha), of early, intermediate, and late ripening, respectively. at fullyripe stage (commercial maturity), blanca cristalina showed the biggest fruits, the hardest texture, and its pulp had the highest protein content. there were no significant differences among morphospeci ...200314609090
urine mutagenicity and lymphocyte dna damage in fruit growers occupationally exposed to the fungicide captan.to determine haematological parameters, urine mutagenicity (on three salmonella typhimurium strains), and dna damage (using the comet assay) in mononuclear leucocytes of farmers before and after a one-day spraying period of pear and apple trees with the fungicide captan in usual conditions.200314634181
morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo.the rapid development of the gastrointestinal tract posthatch has been described; however, little information exists concerning the development of the small intestine in the prehatch period. the present study examined the morphological, cellular, and molecular changes occurring in the small intestine toward the end of the incubation period by examining the expression of intestinal genes that code for brush border digestive enzymes and transporters, their biochemical activities, and the morpholog ...200314653469
sources of polycyclic aromatic hydrocarbon exposure in non-occupationally exposed koreans.urinary 1-hydroxypyrene (1-ohp), an exposure biomarker for polycyclic aromatic hydrocarbons (pahs), was used to identify potential sources of pah exposure for 660 koreans who were not occupationally exposed to pahs (65% male; 35% female; mean age, 36.5 +/- 11.1 years). in this study, 74% of subjects had detectable levels of urinary 1-ohp, with a concentration range of 0.001-3.796 microg/l (median, 0.079 microg/l). a backward elimination was conducted: five variables were selected with a signific ...200314673870
description of paeon asymboli n. sp. (copepoda: sphyriidae), parasitic on asymbolus spp. (catsharks) and a new host record for p. australis kabata, 1993.paeon asymboli n. sp. (copepoda: sphyriidae) is described and illustrated from two female specimens taken from the gills of a grey spotted catshark asymbolus analis (ogilby) and an orange spotted catshark a. rubiginosus (last, gomon & gledhill) (scyliorhinidae) from off southeastern queensland, australia. the key features for identification are: a pear-shaped trunk, longer than it is wide, along with a cephalothorax characterised by two large ventral papillae, projecting laterally and supporting ...200314707509
induction of antimicrobial 3-deoxyflavonoids in pome fruit trees controls fire blight.fire blight, a devastating bacterial disease in pome fruits, causes severe economic losses worldwide. hitherto, an effective control could only be achieved by using antibiotics, but this implies potential risks for human health, livestock and environment. a new approach allows transient inhibition of a step in the flavonoid pathway, thereby inducing the formation of a novel antimicrobial 3-deoxyflavonoid controlling fire blight in apple and pear leaves. this compound is closely related to natura ...200314713146
a myosin inhibitor impairs auxin-induced cell division.the role of myosins for auxin-induced cell division was probed using the inhibitor 2,3-butanedione monoxime in the tobacco cell line vbi-0, where cell elongation and division are axially aligned under the control of auxin. a morphometric analysis revealed that cell division is blocked in a dose-dependent manner, whereas cell expansion continued. in addition, the polarity of terminal cells was impaired resulting in malformed, pear-shaped cells. early effects of the inhibitor are aberrant features ...200314714208
the cytoskeleton of giardia lamblia.giardia lamblia is a ubiquitous intestinal pathogen of mammals. evolutionary studies have also defined it as a member of one of the earliest diverging eukaryotic lineages that we are able to cultivate and study in the laboratory. despite early recognition of its striking structure resembling a half pear endowed with eight flagella and a unique ventral disk, a molecular understanding of the cytoskeleton of giardia has been slow to emerge. perhaps most importantly, although the association of giar ...200312547343
comparative analysis of chloroplast dna in pyrus species: physical map and gene localization.a physical map of chloroplast dna (cpdna) of pear [pyrus ussuriensis var. hondoensis (nakai et kikuchi) rehder] was constructed using five restriction enzymes, sali, xhoi, bamhi, saci and psti. this information will make it possible to investigate the phylogenetic relationships between pyrus species. pear cpdna was found to be a circular molecule with a total size of about 156 kb in which two inverted repeats of 24.8 kb divide the molecule into small (17 kb) and large (90 kb) single-copy regions ...200312582856
involvement of three pathogenicity factors of erwinia amylovora in the oxidative stress associated with compatible interaction in pear.erwinia amylovora, the causal agent of fire blight of maloideae, induces in its susceptible host plants an oxidative burst as does an incompatible pathogen. in this paper we present evidence that the elicitation of this phenomenon is the result of the combined action of two hrp effectors of the bacteria, hrpn and dspa. we also confirmed that desferrioxamine, the siderophore of e. amylovora, is necessary for the bacteria to tolerate high levels of hydrogen peroxide. two other pathogenicity factor ...200312606057
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