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performance on patterned string problems by common marmosets (callithrix jacchus).this experiment examined the performance of common marmosets (callithrix jacchus) on a series of patterned string problems to assess the marmosets' understanding of means-ends relationships. one marmoset, jet, was exposed to a series of problems that were ordered in terms of perceived difficulty during two testings that were separated by 1 year. in the second testing, jet received problems that had been used during the first testing along with three new problems. each of the new problems was des ...201222576582
developmental expression of the pluripotency factor sal-like protein 4 in the monkey, human and mouse testis: restriction to premeiotic germ cells.sall4 (sal-like protein 4) is a pluripotency transcription factor, which is highly expressed in embryonic stem (es) cells and which is essential for mouse preimplantation development. in adult mouse organs, sall4 mrna is highly expressed in the testis and ovary, while there is only little or no expression in other organs. there is also a high expression of sall4 in human testicular germ cell tumors. however, there is as yet no detailed analysis of sall4 expression during mammalian testicular dev ...201222572102
amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina.retinal ganglion cells receive excitatory synapses from bipolar cells and inhibitory synapses from amacrine cells. previous studies in primate suggest that the strength of inhibitory amacrine input is greater to cells in peripheral retina than to foveal (central) cells. a comprehensive study of a large number of ganglion cells at different eccentricities, however, is still lacking. here, we compared the amacrine and bipolar input to midget and parasol ganglion cells in central and peripheral ret ...201222564345
derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells.embryonic and induced pluripotent stem cells (ipscs) derived from mammalian species are valuable tools for modeling human disease, including retinal degenerative eye diseases that result in visual loss. restoration of vision has focused on transplantation of neural progenitor cells (npcs) and retinal pigmented epithelium (rpe) to the retina. here we used transgenic common marmoset (callithrix jacchus) and human pluripotent stem cells carrying the enhanced green fluorescent protein (egfp) reporte ...201222550507
the influence of sex and relatedness on stress response in common marmosets (callithrix jacchus).research in stress physiology has demonstrated the benefits of receiving social support during stressful conditions. however, recent data have shown that the efficacy of social support in buffering physiological and behavioral responses to stressor agents depends on species, sex, and relatedness among animals. this study investigated whether different kinds of social support (presence of same sex related or nonrelated conspecifics) have the same effect on hormonal (fecal cortisol levels) and beh ...201222549557
retinal projections and neurochemical characterization of the pregeniculate nucleus of the common marmoset (callithrix jacchus).in mammals, the suprachiasmatic nucleus (scn) and the intergeniculate leaflet (igl) are the main components of the circadian timing system. the scn is the site of the endogenous biological clock that generates rhythms and synchronizes them to environmental cues. the igl is a key structure that modulates scn activity and is responsible for the transmission of non-photic information to the scn, thus participating in the integration between photic and non-photic stimuli. both the scn and igl receiv ...201222531294
anatomical description and morphometry of the skeleton of the common marmoset (callithrix jacchus).callithrix jacchus (common marmoset) is regularly used in biomedical research, including for studies involving the skeleton. to support these studies, skeletons of healthy animals that had been euthanized for reasons not interfering with skeletal anatomy were prepared. the marmoset dental formula 2i-1c-3p-2m of each oral quadrant is atypical for new world monkeys which commonly possess a third molar. seven cervical, 12-13 thoracic, 7-6 lumbar, 2-3 sacral and 26-29 caudal vertebrae are present, t ...201222517992
aging phenotypes of common marmosets (callithrix jacchus).characterizing the phenotypic changes associated with aging in a short-lived primate is necessary in order to develop better translational models for human health, aging, and disease research. a population of conventionally housed marmoset monkeys was assessed to determine if phenotypes of body composition, hematology, and morphometrical measures were associated with age or risk of death. we found that the cause of mortality in older marmosets was more likely to be due to cardiac and chronic kid ...201222506113
lesions of ventrolateral prefrontal or anterior orbitofrontal cortex in primates heighten negative emotion.heightened fear and anxiety are core symptoms of a variety of neuropsychiatric disorders. they are associated with structural and activity changes throughout neural circuitry that includes the ventral and medial prefrontal cortices (pfc), the amygdala, and hippocampus. although the contributions of the medial pfc, amygdala, and hippocampus to fear and anxiety have been studied extensively with animal models, the selective roles of the ventral pfc-including the ventrolateral prefrontal cortex (vl ...201222502990
comparative anatomy of marmoset and mouse cortex from genomic expression.advances in mouse neural circuit genetics, brain atlases, and behavioral assays provide a powerful system for modeling the genetic basis of cognition and psychiatric disease. however, a critical limitation of this approach is how to achieve concordance of mouse neurobiology with the ultimate goal of understanding the human brain. previously, the common marmoset has shown promise as a genetic model system toward the linking of mouse and human studies. however, the advent of marmoset transgenic ap ...201222496550
evolution of hla-drb genes.the hla region shows diversity concerning the number and content of drb genes present per haplotype. similar observations are made for the equivalent regions in other primate species. to elucidate the evolutionary history of the various hla-drb genes, a large panel of intron sequences obtained from humans, chimpanzees, rhesus macaques, and common marmosets has been subjected to phylogenetic analyses. special attention was paid to the presence and absence of particular transposable elements and/o ...201222826457
conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography: preclinical longitudinal study in common marmosets.conventional magnetic resonance imaging (mri) can detect hemorrhage, edema, syrinx, and spinal cord atrophy, but not axonal disruption after spinal cord injury (sci). we previously demonstrated that diffusion tensor tractography (dtt) could depict axonal disruption after hemisection sci in common marmosets. in the present study, to determine the relationship between dtt results and functional recovery after contusive sci, we performed longitudinal dtt, behavioral, and histological analyses befor ...201222922169
a major metabolite of bupropion reverses motor deficits in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated common marmosets.the atypical antidepressant, bupropion, causes a partial reversal of motor deficits in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated primates. however, its monoamine uptake blocking actions are believed to be mediated by the major metabolites, racemic (-)-(2r,3r)-2-(3-chlorophenyl-3,5,5-trimethyl-2-morphinol) (r,r-hydroxybupropion) and (+)-(2s,3s)-2-(3-chlorophenyl-3,5,5-trimethyl-2-morphinol) (s,s-hydroxybupropion). therefore, we have evaluated the ability of enantiomers to improve locom ...201121522056
conditioned sexual arousal in a nonhuman primate.conditioning of sexual arousal has been demonstrated in several species from fish to humans but has not been demonstrated in nonhuman primates. controversy exists over whether nonhuman primates produce pheromones that arouse sexual behavior. although common marmosets copulate throughout the ovarian cycle and during pregnancy, males exhibit behavioral signs of arousal, demonstrate increased neural activation of anterior hypothalamus and medial preoptic area, and have an increase in serum testoste ...201121029736
influence of different light intensities on the daily grooming distribution of common marmosets callithrix jacchus.the daily distribution of autogrooming was evaluated in adult marmosets submitted to different illumination intensities in the light phase of the light-dark cycle. autogrooming and locomotor activity were monitored and the faecal cortisol level assessed as a stress indicator. the distribution of autogrooming showed two distinct tendencies: when the light intensity varied from 500 to 200 lux, a slight increase in frequency and duration was observed, while a significant decrease in both variables ...201121997701
visualizing myeloarchitecture with magnetic resonance imaging in primates.the pattern of myelination over the cerebral cortex, termed myeloarchitecture, is an established and often-used feature to visualize cortical organization with histology in a variety of primate species. in this paper, we use in vivo magnetic resonance imaging (mri) and advanced image processing using surface rendering to visualize and characterize myeloarchitecture in a small nonhuman primate, the common marmoset (callithrix jacchus). through images made in four female adult marmosets, we produc ...201121599695
possible organizational effect of sex steroids on the stabilization of manual preference in female common marmosets (callithrix jacchus).handedness has been widely studied in nonhuman primates. however, few studies investigate this behavior throughout ontogenetic development. to determine the influence of developmental stage in common marmoset (callithrix jacchus) females, we used six animals, which were observed in food-reaching tests involving hand-use preference from the infantile to the adult phase. during this period, fecal samples were collected for sexual hormone analysis (estrogen and progesterone). the results point to c ...201121553197
a case report of spontaneous opening of congenitally fused labia in a female common marmoset (callithrix jacchus) followed by pregnancy and birth of twins.a first case of spontaneous opening of congenitally fused labia (cfl phenotype) in a captive common marmoset followed by pregnancy and birth is presented here. the occurrence of this phenotype has been previously published in captive marmosets, but so far the etiology is unknown.201121488885
synaptic inputs to two types of koniocellular pathway ganglion cells in marmoset retina.the retinal connectivity of the diverse group of cells contributing to koniocellular visual pathways (widefield ganglion cells) is largely unexplored. here we examined the synaptic inputs onto two koniocellular-projecting ganglion cell types named large sparse and broad thorny cells. ganglion cells were labeled by retrograde tracer injections targeted to koniocellular layer k3 in the lateral geniculate nucleus in marmosets (callithrix jacchus) and subsequently photofilled. retinal preparations w ...201121452222
the marmoset as a model of aging and age-related diseases.the common marmoset (callithrix jacchus) is poised to become a standard nonhuman primate aging model. with an average lifespan of 5 to 7 years and a maximum lifespan of 16½ years, marmosets are the shortest-lived anthropoid primates. they display age-related changes in pathologies that mirror those seen in humans, such as cancer, amyloidosis, diabetes, and chronic renal disease. they also display predictable age-related differences in lean mass, calf circumference, circulating albumin, hemoglobi ...201121411858
a comparison of the imaging performance of high resolution ultrasound scanners for preclinical imaging.nine ultrasound transducers from six ultrasound scanners were assessed for their utility for preclinical ultrasound imaging. the transducers were: l8-16, l10-22 (diasus; dynamic imaging ltd., livingston, uk); l17-5, l15-7io (iu22; philips, seattle, wa, usa), hfl38/13-6 (micromaxx; sonosite inc., bothell, wa, usa); il3lv (vivid 5; ge, fairfield, ct, usa), rmv 704 (vevo 770; visualsonics inc., toronto, canada) and ms550s, ms550d (vevo 2100; visualsonics inc.). a quantitative analysis of the ultras ...201121256667
differential contribution of dietary fat and monosaccharide to metabolic syndrome in the common marmoset (callithrix jacchus).there is a critical need for animal models to study aspects type 2 diabetes (t2d) pathogenesis and prevention. while the rhesus macaque is such an established model, the common marmoset has added benefits including reduced zoonotic risks, shorter life span, and a predisposition to birth twins demonstrating chimerism. the marmoset as a model organism for the study of metabolic syndrome has not been fully evaluated. marmosets fed high-fat or glucose-enriched diets were followed longitudinally to o ...201121164504
differential endocrine responses to infant odors in common marmoset (callithrix jacchus) fathers.olfactory cues can exert priming effects on many mammalian species. paternally experienced marmosets, callithrix jacchus, exposed to direct isolated olfactory contact with their own infant's scent show rapid decreases in testosterone levels within 20 min, whereas paternally inexperienced males do not. the following study tests whether there is a differential steroid response to exposure of infant scent from dependent infants (own and novel) and independent infants (own and novel). we examined th ...201121145893
bipolar input to melanopsin containing ganglion cells in primate retina.two morphological types of melanopsin-expressing ganglion cells have been described in primate retina. both types show intrinsic light responses as well as rod- and cone-driven on-type responses. outer stratifying cells have their dendrites close to the inner nuclear layer (off sublamina); inner stratifying cells have their dendrites close to the ganglion cell layer (on sublamina). both inner and outer stratifying cells receive synaptic input via ribbon synapses, but the bipolar cell types provi ...201120950505
reliability of distortion-product otoacoustic emissions in the common marmoset (callithrix jacchus).this study examines the test-retest reliability of distortion-product otoacoustic emissions (dpoaes) in ketamine-anesthetized common marmosets (callithrix jacchus). dpoae gain functions were measured at 16 f(2)-frequencies between 3 and 24 khz. test-retest reliability was assessed at the following time intervals: (1) interleaved, in which two gain functions were obtained at each frequency before advancing to the next frequency, (2) immediate, wherein one gain function was collected at all f(2)-f ...201121801824
sex identification using the zfx and zfy genes in common marmosets (callithrix jacchus).we investigated sex determination via the zfx and zfy genes using pcr-rflp in the common marmoset. we designed a novel primer set to detect zfx and zfy. a 483-bp band from the zfx gene and a 471-bp band from the zfy gene were amplified. sequencing data of the products amplified from zfx and zfy showed the recognition sites of two restriction enzymes, ddei and msei, respectively. after digestion of the products using each enzyme, we found that the band patterns between females and males were diff ...201121791881
do snakes represent the principal predatory threat to callitrichids? fatal attack of a viper (bothrops leucurus) on a common marmoset (callithrix jacchus) in the atlantic forest of the brazilian northeast.a juvenile common marmoset (callithrix jacchus) was attacked by a whitetail lancehead viper (bothrops leucurus) while playing with other group members close to the ground at a site in northeastern brazil. the attack was almost immediately fatal, but the viper was unable to ingest the body of the marmoset. after approximately 10 min, during which it attempted to swallow the marmoset a number of times, the viper moved away, abandoning the body. while raptors are the principal predators of callitri ...201121766229
finite element analysis of performance in the skulls of marmosets and tamarins.reliance on plant exudates is a relatively rare dietary specialization among mammals. one well-studied example of closely related exudate feeders is the new world marmosets and tamarins. whereas marmosets actively gouge tree bark with their incisors to stimulate the flow of sap, tamarins are opportunistic exudate feeders that do not gouge bark. several studies of the dentaries and jaw adductors indicate that marmosets exhibit specializations for increased gape at the expense of bite force. few s ...201120572898
development of infant common marmosets' (callithrix jacchus) preference for their parents over adults from another group.parent-offspring attachment is important for animals which have offspring that require parental care for their development. infant attachment to the mother has been examined in macaques, but it remains poorly understood in common marmosets. here, we examined the abilities of 14 common marmoset infants to show preference for their parents over adults from another group at the ages of 4, 10, and 15 weeks. each infant was exposed to its parent and an adult from another group in an i-shaped maze. al ...201120567877
chronic colitis in common marmosets (callithrix jacchus) and cotton-top tamarins (saquinus oedipus). 20113122405
kin preference in marmosets and tamarins: saguinus oedipus and callithrix jacchus (callitrichidae, primates).field studies on callitrichid primates have suggested that some species may exhibit a communal breeding system in which two or more males mate with a single female and cooperatively participate in infant rearing. however, high levels of aggression have been observed between males under both laboratory and field conditions. in avian models for communal breeding, cooperating males are often closely related. in a laboratory test of a hypothesis of the amelioration of aggression based on kinship, fo ...20113147598
Allelic Lineages of the Ficolin Genes (FCNs) Are Passed from Ancestral to Descendant Primates.The ficolins recognize carbohydrates and acetylated compounds on microorganisms and dying host cells and are able to activate the lectin pathway of the complement system. In humans, three ficolin genes have been identified: FCN1, FCN2 and FCN3, which encode ficolin-1, ficolin-2 and ficolin-3, respectively. Rodents have only two ficolins designated ficolin-A and ficolin-B that are closely related to human ficolin-1, while the rodent FCN3 orthologue is a pseudogene. Ficolin-2 and ficolin-3 have so ...201122194813
Contribution of the amygdala, but not orbitofrontal or medial prefrontal cortices, to the expression of flavour preferences in marmoset monkeys.The development of food preferences contributes to a balanced diet, and involves both innate and learnt factors. By associating flavour cues with the reinforcing properties of the food (i.e. postingestive nutrient cues and innately preferred tastes, such as sweetness), animals acquire individual preferences. How the brain codes and guides selection when the subject has to choose between different palatable foods is little understood. To investigate this issue, we trained common marmoset monkeys ...201121848920
development of an acute model of inhalational melioidosis in the common marmoset (callithrix jacchus).studies of inhalational melioidosis were undertaken in the common marmoset (callithrix jacchus). following exposure to an inhaled challenge with aerosolized burkholderia pseudomallei, lethal infection was observed in marmosets challenged with doses below 10 cfu; a precise ld(50) determination was not possible. the model was further characterized using a target challenge dose of approximately 10(2) cfu. a separate pathogenesis time-course experiment was also conducted. all animals succumbed, betw ...201122122591
gene targeting and subsequent site-specific transgenesis at the β-actin (actb) locus in common marmoset embryonic stem cells.nonhuman primate embryonic stem (es) cells have vast promise for preclinical studies. genetic modification in nonhuman primate es cells is an essential technique for maximizing the potential of these cells. the common marmoset (callithrix jacchus), a nonhuman primate, is expected to be a useful transgenic model for preclinical studies. however, genetic modification in common marmoset es (cmes) cells has not yet been adequately developed. to establish efficient and stable genetic modifications in ...201121126169
B-cell depletion attenuates white and gray matter pathology in marmoset experimental autoimmune encephalomyelitis.This study investigated the effect of CD20-positive B-cell depletion on central nervous system (CNS) white and gray matter pathology in experimental autoimmune encephalomyelitis in common marmosets, a relevant preclinical model of multiple sclerosis. Experimental autoimmune encephalomyelitis was induced in 14 marmosets by immunization with recombinant human myelin oligodendrocyte glycoprotein in complete Freund adjuvant. At 21 days after immunization, B-cell depletion was achieved by weekly intr ...201122002426
Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG(34-56) peptide in incomplete adjuvant.Induction of experimental autoimmune encephalomyelitis (EAE) has been documented in common marmosets using peptide 34-56 from human myelin/oligodendrocyte glycoprotein (MOG(34-56) ) in incomplete Freund's adjuvant (IFA). Here, we report that this EAE model is associated with widespread demyelination of grey and white matter. We performed an in-depth analysis of the specificity, MHC restriction and functions of the activated T cells in the model, which likely cause EAE in an autoantibody-independ ...201121928277
a small nonhuman primate model for filovirus-induced disease.ebolavirus and marburgvirus are members of the filovirus family and induce a fatal hemorrhagic disease in humans and nonhuman primates with 90% case fatality. to develop a small nonhuman primate model for filovirus disease, common marmosets (callithrix jacchus) were intramuscularly inoculated with wild type marburgvirus musoke or ebolavirus zaire. the infection resulted in a systemic fatal disease with clinical and morphological features closely resembling human infection. animals experienced we ...201121959017
Population parameters of the endangered titi monkey, Callicebus coimbrai Kobayashi and Langguth, 1999, in the fragmented landscape of southern Sergipe, Brazil.The recently-discovered titi, Callicebus coimbrai, is endemic to the coastal Brazilian Atlantic Forest south of the São Francisco River, a region with a long tradition of deforestation. While a number of C. coimbrai populations have now been identified, little is known about basic population parameters. Considering the importance of this information for conservation planning, the population of a site in southern Sergipe (with a total area of forest of approximately 500 ha) was surveyed between A ...201121881780
discrepant effects of human interferon-gamma on clinical and immunological disease parameters in a novel marmoset model for multiple sclerosis.the core pathogenic process in the common marmoset model of multiple sclerosis (ms) is the activation of memory-like t cells specific for peptide 34 to 56 derived from the extracellular domain of myelin/oligodendrocyte glycoprotein (mog(34-56)). immunization with mog(34-56) in incomplete freund's adjuvant is a sufficient stimulus for in vivo activation of these t cells, together with the induction of ms-like disease and cns pathology. ex vivo functional characteristics of mog(34-56) specific t c ...201122012268
vocal control by the common marmoset in the presence of interfering noise.the natural environment is inherently noisy with acoustic interferences. it is, therefore, beneficial for a species to modify its vocal production to effectively communicate in the presence of interfering noises. non-human primates have been traditionally considered to possess limited voluntary vocal control, but little is known about their ability to modify vocal behavior when encountering interfering noises. here we tested the ability of the common marmoset (callithrix jacchus) to control the ...201121993791
development of a novel non-human primate model for rift valley fever.rift valley fever virus (rvfv) can cause severe human disease characterized by either acute-onset hepatitis, or delayed-onset encephalitis, or retinitis and blindness, or a hemorrhagic syndrome. the existing non-human primate (nhp) model for rvf utilizes an intravenous exposure route in rhesus macaques (macaca mulatta). severe disease in these animals is infrequent and large cohorts are needed to observe significant morbidity and mortality. to overcome these drawbacks, we evaluated the infectivi ...201122156530
the (not necessarily) convoluted role of basal radial glia in cortical neurogenesis.recent advances in cell labeling and imaging techniques have dramatically expanded our knowledge of the neural precursor cells responsible for corticogenesis. in particular, radial glial cells are now known to generate several classes of restricted progenitors and neurons. while radial glial cells in the ventricular zone have received the most attention, it has become increasingly clear that a distinct subclass of radial glial cells situated in the subventricular zone (svz) and intermediate zone ...201122116731
toxicity and retinal penetration of infliximab in primates.purpose:: to evaluate the retinal penetration and toxicity of two doses of intravitreal infliximab in primates. methods:: ten marmosets (callithrix jacchus) were given intravitreal injection of 100 μg or 400 μg of infliximab, and balanced salt solution served as control. at baseline and after 24 hours (5 animals) and 7 days (the other 5), the eyes were examined by electroretinography. they were then killed (at 24 hours and 7 days) and assessed by light microscopy and transmission electron micros ...201122080907
Abundant Occurrence of Basal Radial Glia in the Subventricular Zone of Embryonic Neocortex of a Lissencephalic Primate, the Common Marmoset Callithrix jacchus.Subventricular zone (SVZ) progenitors are a hallmark of the developing neocortex. Recent studies described a novel type of SVZ progenitor that retains a basal process at mitosis, sustains expression of radial glial markers, and is capable of self-renewal. These progenitors, referred to here as basal radial glia (bRG), occur at high relative abundance in the SVZ of gyrencephalic primates (human) and nonprimates (ferret) but not lissencephalic rodents (mouse). Here, we analyzed the occurrence of b ...201122114084
behavioral tests as indicator for pain and distress in a primate endometriosis model.as common marmosets (callithrix jacchus) are frequently used experimental animals, sensitive test systems are needed to evaluate impairment and pain caused by procedures and diseases.201121950721
even simple forms of social learning rely on intention attribution in marmoset monkeys (callithrix jacchus).intention attribution guides the cognitively most demanding forms of social learning, such as imitation, thereby scaffolding cumulative cultural evolution. however, it is not thought to be necessary for more basic forms of social learning. here we present evidence that in marmoset monkeys (callithrix jacchus) even most basic forms of social learning such as enhancement depend on intention attribution. marmosets perceived the behavior of a conspecific and a conspecific-like robot, but not that of ...201122103235
Compartmentalization of Cerebral Cortical Germinal Zones in a Lissencephalic Primate and Gyrencephalic Rodent.Previous studies of macaque and human cortices identified cytoarchitectonically distinct germinal zones; the ventricular zone inner subventricular zone (ISVZ), and outer subventricular zone (OSVZ). To date, the OSVZ has only been described in gyrencephalic brains, separated from the ISVZ by an inner fiber layer and considered a milestone that triggered increased neocortical neurogenesis. However, this observation has only been assessed in a handful of species without the identification of the di ...201122114081
intertemporal choice in lemurs.different species vary in their ability to wait for delayed rewards in intertemporal choice tasks. models of rate maximization account for part of this variation, but other factors such as social structure and feeding ecology seem to underly some species differences. though studies have evaluated intertemporal choice in several primate species, including old world monkeys, new world monkeys, and apes, prosimians have not been tested. this study investigated intertemporal choices in three species ...201122024661
Preparation of acute living hippocampal slice from common marmoset (Callithrix jacchus) for synaptic function analysis.We described the preparation of acute living slices from the hippocampus of the neonatal common marmoset (Callithrix jacchus). Slices from a temporal lobe section were prepared quickly using a rotary slicer. By this method, we successfully recorded field potentials, namely, pre-synaptic fiber volley and field excitatory post-synaptic potentials, from the hippocampal CA1 region with conventional electrophysiological techniques, and analyzed the indicators of synaptic function such as input-output ...201122101369
Improving the energy and nutrient supply for common marmoset monkeys fed under long-term laboratory conditions.Background Current knowledge about the optimal energy and nutrient supply for common marmoset monkeys (Callithrix jacchus) is scarce, and more information is needed for establishing the underlying nutritional concepts for facilitating longevity of this species as laboratory animals for biomedical research. Methods Two feeding experiments were conducted to yield fundamental data about feed acceptance, real feed intake, and feed preferences under laboratory conditions. Newly developed feeding co ...201122077415
activity-dependent regulation of mhc class i expression in the developing primary visual cortex of the common marmoset monkey.several recent studies have highlighted the important role of immunity-related molecules in synaptic plasticity processes in the developing and adult mammalian brains. it has been suggested that neuronal mhci (major histocompatibility complex class i) genes play a role in the refinement and pruning of synapses in the developing visual system. as a fast evolutionary rate may generate distinct properties of molecules in different mammalian species, we studied the expression of mhci molecules in a ...201121205317
evolutionary glycomics: characterization of milk oligosaccharides in primates.free oligosaccharides are abundant components of mammalian milk and have primary roles as prebiotic compounds, in immune defense, and in brain development. a mass spectrometry-based technique is applied to profile milk oligosaccharides from apes (chimpanzee, gorilla, and siamang), new world monkeys (golden lion tamarin and common marmoset), and an old world monkey (rhesus). the purpose of this study is to evaluate the patterns of primate milk oligosaccharide composition from a phylogenetic persp ...201121214271
oxytocin changes primate paternal tolerance to offspring in food transfer.oxytocin facilitates social recognition in rats and mice, onset of maternal behavior in virgin mice and formation of pair bonds without copulation in prairie voles. however, the relationship between this peptide and paternal behavior in primates remains largely unknown. we investigated whether oxytocin affects paternal behavior in common marmosets. in these primates, fathers as well as mothers take care of their infants, and transferring food to the infants is one of their more obvious caretakin ...201121225271
effects of constant daylight exposure during early development on marmoset psychosocial behavior.due to global industrialization, the light cycle is shifting to longer daytime. mounting evidence indicates that social developmental disorders may correlate with longer periods of daytime in childhood. however, the exact mechanisms of this link remain unclear. to examine the impact of longer day-time on psychosocial development, we developed a novel non-human primate model, using the common marmoset (callithrix jacchus) reared under constant daylight from birth. marmosets were reared individual ...201121238530
cellular composition and organization of the subventricular zone and rostral migratory stream in the adult and neonatal common marmoset brain.the adult subventricular zone (svz) of the lateral ventricle contains neural stem cells. in rodents, these cells generate neuroblasts that migrate as chains toward the olfactory bulb along the rostral migratory stream (rms). the neural-stem-cell niche at the ventricular wall is conserved in various animal species, including primates. however, it is unclear how the svz and rms organization in nonhuman primates relates to that of rodents and humans. here we studied the svz and rms of the adult and ...201121246550
internet-based atlas of the primate spinal cord.in 2009, we reported an online brain atlas of the common marmoset (callithrix jacchus) at http://marmoset-brain.org:2008. here we report new digital images of the primate spinal cord sections added to the website. we prepared histological sections of every segment of the spinal cord of the common marmoset, rhesus monkey and japanese monkey with various staining techniques. the sections were scanned with carl zeiss mirax scan at light microscopic resolution. obtained digital data were processed a ...201121291922
reading marmoset behavior 'semantics' under particular social context by multi-parameters correlation analysis.social interactions are a fundamental aspect of human and animal behavior. although neuroimaging and other non-invasive methods have progressed recently, the neurobiology of social behavior requires the use of animal models. here, we introduced a multi-behavior parameter integration method and applied it to female-male interaction of adult common marmosets (callithrix jacchus). based on the correlated parameters and meeting context, we found that the behavioral endpoints clustered in four distin ...201121300127
macaque cytochromes p450: nomenclature, transcript, gene, genomic structure, and function.monkeys, especially macaques, including cynomolgus (macaca fascicularis) and rhesus monkeys (macaca mulatta), are frequently used in drug metabolism studies due to their evolutionary closeness to humans. recently, numerous cytochrome p450 (p450 or cyp) cdnas have been identified and characterized in cynomolgus and rhesus monkeys and were named by the p450 nomenclature committee. however, recent advances in genome analysis of cynomolgus and rhesus monkeys revealed that some monkey p450s are appar ...201121303270
measurement of absolute auditory thresholds in the common marmoset (callithrix jacchus).the common marmoset is a small, arboreal, new world primate that has emerged as a promising non-human model system in auditory neuroscience. a complete understanding of the neuroethology of auditory processing in marmosets will include behavioral work examining how sounds are perceived by these animals. however, there have been few studies of the marmoset's hearing and perceptual abilities and the audiogram of this species has not been measured using modern psychophysical methods. the present ex ...201121303689
rapid high-resolution three-dimensional mapping of t1 and age-dependent variations in the non-human primate brain using magnetization-prepared rapid gradient-echo (mprage) sequence.the use of quantitative t(1) mapping in neuroscience and neurology has raised strong interest in the development of t(1)-mapping techniques that can measure t(1) in the whole brain, with high accuracy and precision and within short imaging and computation times. here, we present a new inversion-recovery (ir) based t(1)-mapping method using a standard 3d magnetization-prepared rapid gradient-echo (mprage) sequence. by varying only the inversion time (ti), but keeping other parameters constant, mp ...201121376814
molecular evolution of immunoglobulin superfamily genes in primates.genes of the immunoglobulin superfamily (igsf) have a wide variety of cellular activities. in this study, we investigated molecular evolution of igsf genes in primates by comparing orthologous sequences of 249 igsf genes among human, chimpanzee, orangutan, rhesus macaque, and common marmoset. to evaluate the non-synonymous/synonymous substitution ratio (¤ë), we applied bn-bs program and paml program. igsf genes were classified into 11 functional categories based on the gene ontology (go) databas ...201121390552
the development of small primate models for aging research.nonhuman primate (nhp) aging research has traditionally relied mainly on the rhesus macaque. but the long lifespan, low reproductive rate, and relatively large body size of macaques and related old world monkeys make them less than ideal models for aging research. manifold advantages would attend the use of smaller, more rapidly developing, shorter-lived nhp species in aging studies, not the least of which are lower cost and the ability to do shorter research projects. arbitrarily defining "smal ...201121411860
altered neural responses to sounds in primate primary auditory cortex during slow-wave sleep.how sounds are processed by the brain during sleep is an important question for understanding how we perceive the sensory environment in this unique behavioral state. while human behavioral data have indicated selective impairments of sound processing during sleep, brain imaging and neurophysiology studies have reported that overall neural activity in auditory cortex during sleep is surprisingly similar to that during wakefulness. this responsiveness to external stimuli leaves open the question ...201121414918
protamine-1 represents a sperm specific gene transcript: a study in callithrix jacchus and bos taurus.spermatozoa are transcriptionally inactive cells, but contain acetylated histones, normally a characteristic of transcriptionally active cells. acetylgroups are thought to represent epigenetic marks that are transmitted to the oocyte and are involved in starting gene expression in the zygote and in regulating gene expression during early embryogenesis. we performed reverse transcription polymerase chain reaction (rt-pcr) in the common marmoset monkey (callithrix jacchus) and in bovine spermatozo ...201121486395
comparative genome analysis of the major histocompatibility complex (mhc) class i b/c segments in primates elucidated by genomic sequencing in common marmoset (callithrix jacchus).common marmoset monkeys (callithrix jacchus) have emerged as important animal models for biomedical research, necessitating a more extensive characterization of their major histocompatibility complex (mhc) region. however, the genomic information of the marmoset mhc (caja) is still lacking. the mhc-b/c segment represents the most diverse mhc region among primates. therefore, in this paper, to elucidate the detailed gene organization and evolutionary processes of the caja class i b (caja-b) segme ...201121505866
characterization of bcar4, a novel oncogene causing endocrine resistance in human breast cancer cells.resistance to the antiestrogen tamoxifen remains a major problem in the management of estrogen receptor-positive breast cancer. knowledge on the resistance mechanisms is needed to develop more effective therapies. breast cancer antiestrogen resistance 4 (bcar4) was identified in a functional screen for genes involved in tamoxifen resistance. bcar4 is expressed in 27% of primary breast tumors. in patients treated with tamoxifen for metastized disease high bcar4 mrna levels are associated with red ...201121506106
post-exposure therapy of inhalational anthrax in the common marmoset.the aim of this study was to compare the pharmacokinetics and efficacy of ciprofloxacin as post-exposure therapy against inhalational anthrax in the common marmoset (callithrix jacchus) with other non-human primate models in order to determine whether the marmoset is a suitable model to test post-exposure therapies for anthrax. pharmacokinetic (pk) and efficacy studies with ciprofloxacin were performed in the marmoset. ciprofloxacin plasma pharmacokinetics were determined in six animals in separ ...201121530184
development of a compact and general-purpose experimental apparatus with a touch-sensitive screen for use in evaluating cognitive functions in common marmosets.common marmosets have been used extensively in biomedical research and the recent advent of techniques to generate transgenic marmosets has accelerated the use of this model. new methods that efficiently assess the degree of cognitive function in common marmosets are needed in order to establish their suitability as non-human primate models of higher brain function disorders. here, we have developed a new apparatus suitable for testing the cognitive functions of common marmosets. utilizing a min ...201121549751
inhibition of maternal behaviour by central infusion of corticotrophin-releasing hormone in marmoset monkeys.stress can inhibit maternal behaviour and increase rates of child abuse in humans and other animals; however, the neuroendocrine mechanisms are not known. to determine whether corticotrophin-releasing hormone (crh) plays a role in stress-induced disruption of maternal behaviour in primates, we characterised the effects of acute intracerebroventricular (icv) infusions of crh on maternal and abusive behaviour in common marmoset monkeys (callithrix jacchus). nulliparous females were implanted with ...201121554432
establishment of the milk-borne transmission as a key factor for the peculiar endemicity of human t-lymphotropic virus type 1 (htlv-1): the atl prevention program nagasaki.in late 2010, the nation-wide screening of pregnant women for human t-lymphotropic virus type 1 (htlv-1) infection was implemented in japan to prevent milk-borne transmission of htlv-1. in the late 1970s, recognition of the adult t-cell leukemia (atl) cluster in kyushu, japan, led to the discovery of the first human retrovirus, htlv-1. in 1980, we started to investigate mother-to-child transmission (mtct) for explaining the peculiar endemicity of htlv-1. retrospective and prospective epidemiolog ...201121558754
characterization of 3,4-methylenedioxymethamphetamine (mdma) enantiomers in vitro and in the mptp-lesioned primate: r-mdma reduces severity of dyskinesia, whereas s-mdma extends duration of on-time.l-3,4-dihydroxyphenylalanine (l-dopa) is the most effective treatment for parkinson's disease, but long-term l-dopa administration is marred by the emergence of motor complications, namely, dyskinesia and a shortening of antiparkinsonian benefit (wearing-off). 3,4-methylenedioxymethamphetamine (mdma) is unique in that it exerts antidyskinetic effects and may enhance antiparkinsonian actions of l-dopa. mdma is composed of two enantiomers with different pharmacological profiles; here, we describe ...201121562283
hormonal and behavioral responses to stress in lactating and non-lactating female common marmosets (callithrix jacchus).in several mammalian species, hypothalamic-pituitary-adrenal (hpa) and behavioral responses to stressors are down-regulated in lactating females, possibly preventing stress-induced disruptions of maternal care. experimental elevations of hpa axis hormones have been found to inhibit maternal behavior in lactating common marmoset monkeys (callithrix jacchus), raising the question of whether lactating female marmosets also have blunted endogenous responses to stress. therefore, we compared hpa and ...201121600906
distinct structural plasticity in the hippocampus and amygdala of the middle-aged common marmoset (callithrix jacchus).adult neurogenesis in the primate brain is generally accepted to occur primarily in two specific areas; the subgranular zone (sgz) of the hippocampal dentate gyrus (dg) and the subventricular zone (svz) of the lateral ventricles. hippocampal neurogenesis is well known to be downregulated by stress and aging in rodents, however there is less evidence documenting the sensitivity of neuroblasts generated in the svz. in primates, migrating cells generated in the svz travel via a unique temporal stre ...201121605555
fine frequency tuning in monkey auditory cortex and thalamus.the frequency resolution of auditory neurons throughout the ascending auditory pathway is an important parameter for understanding how sounds are processed. it has been shown in previous animal studies that the frequency tuning widths are about 1/5 octave wide in auditory nerve fibers and much wider in auditory cortex neurons (pickles, 1979; mclaughlin et al., 2008; see table 1). psychophysical studies show that humans are capable of discriminating far finer frequency differences (oxenham and sh ...201121613589
modeling hippocampal neurogenesis across the lifespan in seven species.the aim of this study was to estimate the number of new cells and neurons added to the dentate gyrus across the lifespan, and to compare the rate of age-associated decline in neurogenesis across species. data from mice (mus musculus), rats (rattus norvegicus), lesser hedgehog tenrecs (echinops telfairi), macaques (macaca mulatta), marmosets (callithrix jacchus), tree shrews (tupaia belangeri), and humans (homo sapiens) were extracted from 21 data sets published in 14 different reports. analysis ...201121621300
[risks of transmitting rabies virus from captive domiciliary common marmoset (callithrix jacchus) to human beings, in the metropolitan region of fortaleza, state of ceará, brazil.] 201121625806
evolution of the cyp2d gene cluster in humans and four non-human primates.the human cytochrome p450 2d6 (cyp2d6) is a primary enzyme involved in the metabolism of about 25% of commonly used therapeutic drugs. cyp2d6 belongs to the cyp2d subfamily, a gene cluster located on chromosome 22, which comprises the cyp2d6 gene and pseudogenes cyp2d7p and cyp2d8p. although the chemical and physiological properties of cyp2d6 have been extensively studied, there has been no study to date on molecular evolution of the cyp2d subfamily in the human genome. such knowledge could grea ...201121670550
decision making and risk attitude of the common marmoset in a gambling task.to analyze decision making under uncertainty of monkeys, common marmosets were trained to choose and remove one of two colored caps on wells arranged side by side. each well contained constant reward (3 grains of puffed rice) or risky reward (0 or 6 grains; probability, 50%:50%). for each marmoset, white or black color was assigned randomly as a symbol of non-risky or risky choice. arrangement of white and black caps was determined randomly in each trial. after 200 trials (5 trials per day), the ...201121802456
the pathology of experimental poxvirus infection in common marmosets (callithrix jacchus): further characterization of a new primate model for orthopoxvirus infections.zoonotic orthopoxvirus (opv) can induce severe disease in man and the virus has potential for use in bioterrorism. new vaccines and therapeutics against opv infections must be tested in animal models. the aim of this study was to characterize the clinical course and pathology of a new opv isolate, calpox virus, which is infectious in marmosets. infection experiments were performed with 28 common marmosets (callithrix jacchus) exposed to different challenge doses of calpox virus by the intravenou ...201121783202
increased positive selection pressure within the complementarity determining regions of the t-cell receptor ß gene in new world monkeys.because of the long-term co-evolution of tcr and mhc molecules, numerous nucleotide substitutions have accumulated within the domains of tcrß genes. we previously found that nonsynonymous nucleotide substitutions occurred more frequently in complementarity determining region (cdr)ß than in cdra, even though only a limited number of common marmoset (callithrix jacchus) and human t-cell receptor ß variable (trbv) sequences were compared. this interesting finding raised the question of whether the ...201121769905
tool-use learning by common marmosets (callithrix jacchus).one of the most critical and common features of tool use is that the tool essentially functions as a part of the body. this feature is likely rooted in biological features that are shared by tool users. to establish an ideal primate model to explore the neurobiological mechanisms supporting tool-use behaviours, we trained common marmosets, a small new world monkey species that is not usually associated with tool use, to use a rake-shaped tool to retrieve food. five naive common marmosets were sy ...201121713504
quantification of zp1, zp2 and zp3 mrna of marmoset monkey (callithrix jacchus) oocytes from periantral and antral follicles.in mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (zp) consisting mainly of zp glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. the zp proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. as little is known about the stage-dependent expression of zp1, zp2 and zp3 in marmoset monkey (callithrix jacchus) oocytes, mrna ...201121689136
triiodothyronine administration ameliorates the demyelination/remyelination ratio in a non-human primate model of multiple sclerosis by correcting tissue hypothyroidism.remyelination failure is a key landmark in chronic progression of multiple sclerosis (ms), the most diffuse demyelinating disease in human, but the reasons for this are still unknown. it has been shown that thyroid hormone administration in the rodent models of acute and chronic demyelinating diseases improved their clinical course, pathology and remyelination. in the present study, we translated this therapeutic attempt to experimental allergic encephalomyelitis (eae) in the non-human primate c ...201121707794
object permanence in adult common marmosets (callithrix jacchus): not everything is an "a-not-b" error that seems to be one.in this paper, we describe a behaviour pattern similar to the "a-not-b" error found in human infants and young apes in a monkey species, the common marmosets (callithrix jacchus). in contrast to the classical explanation, recently it has been suggested that the "a-not-b" error committed by human infants is at least partially due to misinterpretation of the hider's ostensively communicated object hiding actions as potential 'teaching' demonstrations during the a trials. we tested whether this so- ...201121739135
colonization with nontuberculous mycobacteria is associated with positive tuberculin skin test reactions in the common marmoset (callithrix jacchus).mycobacterium tuberculosis infections can result in significant morbidity and mortality in nonhuman primate colonies. preventative health programs designed to detect infection routinely include tuberculin skin testing (tst). because mammalian old tuberculin used for tst contains antigens common to a variety of mycobacterial species, false-positive results can occur in animals sensitized to nontuberculous mycobacteria (ntm). over 11 mo, a large colony of common marmosets (callithrix jacchus) demo ...201121819699
a novel two promoter- one gene system of the chorionic gonadotropin beta (cgb) gene enables tissue specific expression.the new world monkey (nwm), callithrix jacchus, a preferred model in medical research, displays an interesting endocrine regulation of reproduction: the heterodimeric glycoprotein hormone luteinizing hormone (lh) is functionally replaced by the chorionic gonadotropin (cg), a hormone indispensable for establishment of pregnancy in humans and normally expressed in the placenta. in the marmoset pituitary, the expression of the beta-subunit (cgb) gene is regulated similarly to human lh beta-subunit, ...201121821715
ultrastructural analysis, zinc transporters, glucose transporters and hormones expression in new world primate (callithrix jacchus) and human pancreatic islets.the new world primates (nwp) callithrix jacchus separated from man approximately 50million years ago and is a potential alternative small non-human primate model for diabetes research. ultrastructure, and gene expression of pancreatic islets and the recently described diabetes auto antigenic zinc transporters families in human, nwp and pig pancreas were studied. morphologically nwp islets were larger than pig islets and similar in size to human islets. nwp islets alpha cells had high dense core ...201121821034
oxalate nephropathy in a laboratory colony of common marmoset monkeys (callithrix jacchus) following the ingestion of eucalyptus viminalis.seven common marmoset monkeys (callithrix jacchus) from a laboratory colony of 17 died over a period of eight months. death of six of these monkeys was attributed to kidney failure from an oxalate-induced nephropathy. the epidemiology of this outbreak suggested an exogenous source and there was strong evidence that the source was bark and leaves from an eucalyptus viminalis tree. branches of this tree were introduced one month before the first death. the branches were removed one month after dea ...201121727186
colonization of collagen scaffolds by adipocytes derived from mesenchymal stem cells of the common marmoset monkey.in regenerative medicine, human cell replacement therapy offers great potential, especially by cell types differentiated from immunologically and ethically unproblematic mesenchymal stem cells (mscs). in terms of an appropriate carrier material, collagen scaffolds with homogeneous pore size of 65++m were optimal for cell seeding and cultivating. however, before clinical application and transplantation of msc-derived cells in scaffolds, the safety and efficiency, but also possible interference in ...201121726528
bronchoconstriction in nonhuman primates: a species comparison.bronchoconstriction is a characteristic symptom of various chronic obstructive respiratory diseases such as chronic obstructive pulmonary disease and asthma. precision-cut lung slices (pcls) are a suitable ex vivo model to study physiological mechanisms of bronchoconstriction in different species. in the present study, we established an ex vivo model of bronchoconstriction in nonhuman primates (nhps). pcls prepared from common marmosets, cynomolgus macaques, rhesus macaques, and anubis baboons w ...201121700889
common marmoset (callithrix jacchus) as a primate model of dengue virus infection: development of high levels of viremia and demonstration of protective immunity.dengue virus (denv) causes a wide range of illness in humans: dengue fever (df), dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss). animal models that constantly develop high levels of viremia are required for the development of protective and preventive measures. common marmosets (callithrix jacchus) demonstrated high levels of viremia after inoculation with clinical isolates of four serotypes of denv; in particular, over 10(6) genome copies/ml after inoculation with denv-2. nonstr ...201121697346
a case of intestinal mucormycosis in a common marmoset (callithrix jacchus).a 3-year-old female common marmoset was euthanatized because of wasting. grossly, a perforating lesion was present in the jejunum and hemorrhagic lesions in the cecum and colon. histopathologically, these gross lesions were a perforated jejunal ulcer and necrotizing colitis, respectively. necrotizing colitis was characterized by extensive mucosal necrosis along with numerous ribbon-shaped aseptate hyphae. these aseptate hyaline hyphae were positively stained with pas and gms, and reacted immunoh ...201122020147
Comparative distribution of cocaine- and amphetamine-regulated transcript (CART) in the hypothalamus of the capuchin monkey (Cebus apella) and the common marmoset (Callithrix jacchus).Cocaine- and amphetamine-regulated transcript (CART) is widely distributed in the brain of many species. In the hypothalamus, CART neurotransmission has been implicated in diverse functions including energy balance, stress response, and temperature and endocrine regulation. Although some studies have been performed in primates, very little is known about the distribution of CART neurons in New World monkeys. New World monkeys are good models for systems neuroscience, as some species have evolved ...201122030409
Effects of rearing conditions on early visual development in common marmosets.Early development of visual behavior was examined in hand-reared (HR) and parentally reared (PR) common marmosets (Callithrix jacchus). On the day of birth, most of the marmosets exhibited sensitivity to light and sound: they closed their eyes in response to light and oriented to the sound sources. The behavior of tracking moving visual stimuli was exhibited at around 10 days postnatally in PR marmosets, but the onset of this behavior was delayed to the age of 16 days in HR marmosets. The delay ...201122127827
human hepatocyte growth factor promotes functional recovery in primates after spinal cord injury.many therapeutic interventions for spinal cord injury (sci) using neurotrophic factors have focused on reducing the area damaged by secondary, post-injury degeneration, to promote functional recovery. hepatocyte growth factor (hgf), which is a potent mitogen for mature hepatocytes and a mediator of the inflammatory responses to tissue injury, was recently highlighted as a potent neurotrophic factor in the central nervous system. we previously reported that introducing exogenous hgf into the inju ...201122140459
brevity is not always a virtue in primate communication.semple et al. (semple et al. in press, biol. lett. (doi:10.1098/rsbl.2009.1062)) argued that the 'law of brevity' (an inverse relationship between word length and frequency of use) applies not only to human language but also to vocal signalling in non-human primates, because coding efficiency is paramount in both situations. we analysed the frequency of use of signals of different duration in the vocal repertoires of two neotropical primate species studied in the wild-the common marmoset (callit ...201120573617
the developmental impact of prenatal stress, prenatal dexamethasone and postnatal social stress on physiology, behaviour and neuroanatomy of primate offspring: studies in rhesus macaque and common marmoset.exposure of the immature mammalian brain to stress factors, including stress levels of glucocorticoids, either prenatally or postnatally, is regarded as a major regulatory factor in short- and long-term brain function and, in human, as a major aetiological factor in neuropsychiatric disorders. experimental human studies are not feasible and animal studies are required to demonstrate causality and elucidate mechanisms. a number of studies have been conducted and reviewed in rodents but there are ...201120809212
modulation of physical understanding by common marmosets (callithrix jacchus).the understanding of physical causality in common marmosets was tested using support problems in which a pair of sheets was presented to determine whether subjects would choose the sheet that had a food item on it (i.e., the sheet was supporting the food item). in two experiments, the conditions were manipulated in terms of the length of the sheet, the distance between the sheet and the food item, the presence of a gap separating the two sheets, and the size of the food item. in experiment 1, th ...201120830498
the bony labyrinth of the early platyrrhine primate chilecebus.we document the morphology of the bony labyrinth of chilecebus carrascoensis, one of the best preserved early platyrrhines known, based on high resolution ct scanning and 3d digital reconstruction. the cochlea is low and conical in form, as in other anthropoids, but has only 2.5 spiral turns. when the allometric relationship with body mass is considered, cochlear size is similar to that in extant primates. the relative size of the semicircular canals, which is well within the range of other prim ...201020952046
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