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variation in competence for zikv transmission by aedes aegypti and aedes albopictus in mexico.zikv is a new addition to the arboviruses circulating in the new world, with more than 1 million cases since its introduction in 2015. a growing number of studies have reported vector competence (vc) of aedes mosquitoes from several areas of the world for zikv transmission. some studies have used new world mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the asian lineage of zikv.201829965958
chikungunya in children: a clinical review.chikungunya (chikv) is an emerging arboviral infection with recent spikes in transmission in the americas. chikungunya is most commonly transmitted by mosquitos, specifically aedes aegypti and aedes albopictus. these mosquitoes are found throughout many parts of the united states. the classic tetrad of symptoms for chikv is fever, symmetric polyarthralgia, maculopapular rash, and nonpurulent conjunctivitis. although the majority (3 of 4) of infected people will be symptomatic, the viral illness ...201829965819
adventitious viruses persistently infect three commonly used mosquito cell lines.mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. accordingly, we screened four commonly-used mosquito cell lines, c6/36 and u4.4 cells from aedes albopictus, aag2 cells from aedes aegypti, and hsu cells from culex quinquefasciatus, for the presence of adventitious (i.e. exogenous ...201829957338
patterns of occurrence of dengue and chikungunya, and spatial distribution of mosquito vector aedes albopictus in swabi district, pakistan.two principal vector species, aedes aegypti and aedes albopictus, are known for transmission of dengue (den) and chikungunya (chk) in pakistan. we aimed to investigate their spatial and temporal distribution.201829956428
empirical optimization of risk thresholds for dengue: an approach towards entomological management of aedes mosquitoes based on larval indices in the kandy district of sri lanka.larval indices such as premise index (pi), breteau index (bi) and container index (ci) are widely used to interpret the density of dengue vectors in surveillance programmes. these indices may be useful for forecasting disease outbreaks in an area. however, use of the values of these indices as alarm signals is rarely considered in control programmes. therefore, the current study aims to propose threshold values for vector indices based on an empirical modeling approach for the kandy district of ...201829954443
comparison of chikungunya viruses generated using infectious clone or the infectious subgenomic amplicons (isa) method in aedes mosquitoes.reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study rna viruses and to develop new antiviral compounds and vaccine strategies. the recently described method called isa (infectious subgenomic amplicons) gives the possibility to rescue rna viruses in days. we demonstrated in cell culture that the use of the isa method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. however, no r ...201829953474
aedes anphevirus: an insect-specific virus distributed worldwide in aedes aegypti mosquitoes that has complex interplays with wolbachia and dengue virus infection in cells.insect-specific viruses (isvs) of the yellow fever mosquito aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (denv) and west nile virus by the mosquito. the diversity and composition of the virome of a. aegypti, however, remains poorly understood. in this study, we characterized aedes anphevirus (aeav), a negative-sense rna virus from the order mononegavirales aeav identified from aedes cell lines was infectious to both a. aegypti and aedes albopi ...201829950416
arthropod evs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein tsp29fb.dengue virus (denv) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. using in vitro cell lines derived from aedes albopictus and aedes aegypti, the primary vectors of denv, we report that denv2/denv3-infected cells secrete extracellular vesicles (evs), including exosomes, containing infectious viral rna and proteins. a full-length denv2 genome, detected in arthropod evs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and bloo ...201829946031
model-based analysis of experimental data from interconnected, row-configured huts elucidates multifaceted effects of a volatile chemical on aedes aegypti mosquitoes.insecticides used against aedes aegypti and other disease vectors can elicit a multitude of dose-dependent effects on behavioral and bionomic traits. estimating the potential epidemiological impact of a product requires thorough understanding of these effects and their interplay at different dosages. volatile spatial repellent (sr) products come with an additional layer of complexity due to the potential for altered movement of affected mosquitoes and diffusion of volatile particles of the produ ...201829941038
wolbachia enhances insect-specific flavivirus infection in aedes aegypti mosquitoes.mosquitoes transmit a diverse group of human flaviviruses including west nile, dengue, yellow fever, and zika viruses. mosquitoes are also naturally infected with insect-specific flaviviruses (isfs), a subgroup of the family not capable of infecting vertebrates. although isfs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits t ...201829938064
using barcoded zika virus to assess virus population structure in vitro and in aedes aegypti mosquitoes.arboviruses such as zika virus (zikv, flaviviridae; flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. to help quantify these bottlenecks and their effects, we constructed 4 "barcoded" zikv populations that theoretically contain thousands of barcodes each. after identifying ...201829935423
circulation of chikungunya virus in aedes aegypti in maranhão, northeast brazil.the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv) arboviruses have placed brazil among the main worldwide endemic areas. brazilian northeast region concentrates the highest incidence of infections caused by chikv and zikv. in maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of aedes aegypti and aedes albopictus vectors. in this context, this study aimed to investi ...201829932932
ancient treatment for lice: a source of suggestions for carriers of other infectious diseases?louse infestation is one of the oldest contagious pestilential diseases of humankind, which has recently re-emerged in several developing countries as well as in homeless individuals and migrants. the present work provides the first phase of an historical excursus of louse remedies based on the classics of pharmaceutical literature, codes, pharmacopoeia and treatises. the second phase involves a literature search, based on the principal medical databases (scifinder, pubmed, google scholar, isi-w ...201829932096
evaluation of aedes aegypti (diptera: culicidae) life table attributes upon chikungunya virus replication reveals impact on egg-laying pathways.arthropod-borne virus disease cycles constitute interactions among three primary players-the host, the vector, and the virus-in which the virus needs to interact with the host and the vector to establish its survival. while in the host, some arboviruses replicate aggressively, resulting in host pathogenicity, and manifest as a disease condition. these viruses more often utilize the vectors as reservoirs before they are transmitted to the host and therefore do not amplify to as large titers as th ...201829931258
a high-risk zika and dengue transmission hub: virus detections in mosquitoes at a brazilian university campus.zika virus (zikv) and dengue virus (denv) are mosquito-borne flaviviruses prevalent throughout tropical regions. currently, management of zikv and denv centers on control of the primary vector aedes aegypti. this vector is highly anthropophilic and is therefore prevalent throughout densely urbanised landscapes. a new passive trap for gravid ae. aegypti (gravid aedes trap - gat) was developed for mosquito surveillance. here the different killing agents and the level of transmission of arboviruses ...201829929561
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
identification of aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects.sophisticated tools for manipulation of gene expression in select neurons, including neurons that regulate sexually dimorphic behaviors, are increasingly available for analysis of genetic model organisms. however, we lack comparable genetic tools for analysis of non-model organisms, including aedes aegypti, a vector mosquito which displays sexually dimorphic behaviors that contribute to pathogen transmission. formaldehyde-assisted isolation of regulatory elements followed by sequencing (faire-se ...201829996889
tropomyosins in mosquito and house dust mite cross-react at the humoral and cellular level.aedes aegypti and dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. however, the functional and clinical relevance of their cross-reactivity is still debated.201829992665
mosquito arbovirus survey in selected areas of kenya: detection of insect-specific virus.many arboviral outbreaks have occurred in various locations in kenya. entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. therefore, mosquitoes were collected in kenya to determine the distribution of arboviruses.201829991925
environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties.green synthesis of nanoparticles can be an important alternative compared to conventional physio-chemical synthesis. we utilized scadoxus multiflorus leaf powder aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (zno nps). further, the synthesized zno nps were subjected to various characterization techniques. transmission electron microscope (tem) analysis showed an irregular spherical shape, with an average particle size of 31 ± 2 nm. furtherm ...201829986435
molecular aspects of the dengue virus infection process: a review.at the present time, dengue is one of the most important arboviruses affecting man, becoming a serious global public health problem, especially in subtropical and tropical countries, where environmental conditions favor the development and proliferation of the mosquito aedes aegypti. dengue is caused by a type of flavivírus, which is an enveloped virus of spherical geometry. nowadays, it is one of the diseases with the highest incidence in brazil, reaching the population of all states, regardles ...201829984641
aedes aegypti in the black sea: recent introduction or ancient remnant?the yellow fever mosquito aedes aegypti transmits viral diseases that have plagued humans for centuries. its ancestral home are forests of africa and ~400-600 years ago it invaded the new world and later europe and asia, causing some of the largest epidemics in human history. the species was rarely detected in countries surrounding the mediterranean sea after the 1950s, but during the last 16 years it re-appeared in madeira, russia and in the eastern coast of the black sea. we genotyped ae. aegy ...201829980229
west nile virus and other mosquito-borne viruses present in eastern europe.eastern europe (ee) has been severely affected by mosquito-borne viruses (moboviruses). in this review, we summarize the epidemiology of moboviruses, with particular attention to west nile virus (wnv). the study of wnv human cases in ee between 2010 and 2016, revealed that the epidemiology of wnv in ee is complex with the combination of introduction of different wnv strains from lineages 1 and 2, and the establishment of endemic cycles. we found a positive correlation between the risk of wnv re- ...201829979950
both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.we recently reported that larval stage aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. we further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription factors and other processes larvae require for growth. in this study we assessed whether other types of organisms induce mosquito larvae to grow and asked if the density of non-living mi ...201829979680
variation in temephos resistance in field populations of aedes aegypti (diptera: culicidae) in the state of sergipe, northeast brazil.the organophosphate temephos was first used in brazil in the 1960s for the control of aedes aegypti. because of its extensive and longstanding use worldwide, selection for mosquito populations resistant to the chemical has been observed not only in different regions of brazil but also in several parts of the world. the objective of this study was to evaluate the susceptibility of ae. aegypti to the organophosphate temephos, a larvicide used in vector control activities in sergipe/northeast brazi ...201829972557
zika virus: - a review of the main aspects of this type of arbovirosis.the objective of this study was to integrate findings related to zika virus from the scientific literature. an integrative review of medline was conducted, and data search was performed using the health sciences descriptor zika virus and the following filters: full texts available; studies in humans; full articles; and publications in portuguese, french, english, and spanish. after application of the filters, followed by reading of the titles, abstracts, and full texts, 44 studies were included ...201829972554
modelling the distribution of the vector aedes aegypti in a central argentine city.aedes aegypti (diptera: culicidae) is an urban mosquito involved in the transmission of numerous viruses, including dengue, chikungunya and zika. in argentina, ae. aegypti is the main vector of dengue virus and has been involved in several outbreaks in regions ranging from northern to central argentina since 2009. in order to evaluate areas of potential vector-borne disease transmission in the city of córdoba, argentina, the present study aimed to identify the environmental, socioeconomic and de ...201830027565
assessment of insecticide resistance in primary dengue vector, aedes aegypti (linn.) from northern districts of west bengal, india.aedes mosquitoes are the major vectors transmitting several arboviral diseases such as dengue, zika and chikungunya worldwide. northern districts of west bengal is home to several epidemics vectored by mosquito including dengue infections, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches. this study was carried out to unveil the level of insecticide resistance prevailing among the primary ...201830026024
copy number variation (cnv) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?copy number variation (cnv) in insect genomes is a rich source of potentially adaptive polymorphism which may help overcome the constraints of purifying selection on conserved genes and/or permit elevated transcription. classic studies of amplified esterases and acetylcholinesterase duplication in culex pipiens quantified evolutionary dynamics of cnv driven by insecticidal selection. a more complex and potentially medically impactful form of cnv is found in anopheles gambiae, with both heterogen ...201830025639
melanization of dirofilaria immitis larvae in different culicid species.dirofilaria immitis is an important filarioid transmitted by culicids. the vector role of these arthropods may be influenced by biological events as melanization against d. immitis larvae. this study aimed to detect the occurrence of melanization in culex quinquefasciatus, aedes (stegomyia) aegypti and aedes (stegomyia) albopictus experimentally infected with d. immitis.201830018997
the use of insecticide-treated curtains for control of aedes aegypti and dengue virus transmission in "fraccionamiento" style houses in méxico.dengue, chikungunya, yellow fever, and zika viruses transmitted by aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. in méxico, construction of large tracts of "fraccionamientos" high density housing to accommodate population growth and urbanization has provided fertile ground for ae. aegypti-transmitted viruses. we investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of ae. aegypti and to prevent dengue virus ( ...201830018645
zika virus outbreak in the pacific: vector competence of regional vectors.in 2013, zika virus (zikv) emerged in french polynesia and spread through the pacific region between 2013 and 2017. several potential aedes mosquitoes may have contributed to the zikv transmission including aedes aegypti, the main arbovirus vector in the region, and aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. the aim of this study was to analyze the ability of these two pacific vectors to transmit zikv at a regional scale, through the evaluation and ...201830016372
molecular characterization of the cry gene profile of bacillus thuringiensis isolated from a caribbean region of colombia.in order to characterize native strains of bacillus thuringiensis of the colombian caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as b. thuringiensis were isolated from different soils and muds around the city of valledupar. using a biological test, five isolates of b. thuringiensis showed toxic effect against larvae of aedes aegypti. pcr methods were used to detect cry1, cry2, cry4b, cry10 and cyt1 genes. cry1 and cry2 genes were detected in 35.7% and 32.1 ...201830015421
evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against aedes aegypti l. (diptera: culicidae).in a previous screening program for mosquitocides from local edible plants in thailand, essential oils (eos) of cyperus rotundus, alpinia galanga and cinnamomum verum, were found to possess promising adulticidal activity against aedes aegypti. with the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of eos on permethrin toxicity against ae. a ...201830005688
epidemiological significance of dengue virus genetic variation in mosquito infection dynamics.the kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant of arbovirus epidemiology. the time interval between virus acquisition and transmission by the vector, termed extrinsic incubation period (eip), combines with vector mortality rate and vector competence to determine the proportion of infected vectors that eventually become infectious. however, the dynamic nature of this process, and the amount of natural variation i ...201830005085
the c-type lectin domain gene family in aedes aegypti and their role in arbovirus infection.several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the c-type lectin fold that is present in either vertebrate or invertebrate proteins. while in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. despite the establishment of mosquito c-type lectin domain containing proteins (ctldcps) as known host factors in assisting the infectious process ...201830002303
optimization of cationic (q)-paper for detection of arboviruses in infected mosquitoes.previously (glushakova et al. 2017), a cellulose-based cationic (q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (nas) derived from mosquito vectors infected with pathogens for surveillance. nas bind electrostatically to q-paper, but the quantity of na bound depends on the paper's binding capacity. to optimize the original technology for mosquito surveillance, factors that affected na absorbance on q-paper w ...201830099053
discovery and high prevalence of phasi charoen-like virus in field-captured aedes aegypti in south china.arboviruses have caused significant global health concerns during the past decade. in this regard, continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. here, a novel isolate of phasi charoen-like virus (pclv zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in zhanjiang by next generation sequencing. phylogenetic analysis suggested that pclv zhanjiang01 belonged to the genus phasivirus in the family phenuiviridae. the ...201830077072
managing aedes aegypti populations in the first zika transmission zones in the continental united states.the african zika virus swept across the pacific, reaching the new world in 2014. in july, 2016, miami-dade county, florida became the locus of the first mosquito-borne zika transmission zones in the continental united states. control efforts were guided by the centers for disease control and prevention, including aerial and truck sprays of adulticides and larvicides. to improve our understanding of how best to fight zika transmission in an urban environment in the developed world, trap counts of ...201830075097
genetic characterization of chikungunya viruses isolated during the 2015-2017 outbreaks in different states of india, based on their e1 and e2 genes.during 2015-2017, chikungunya virus (chikv) showed a resurgence in several parts of india with karnataka, maharashtra and new delhi accounting for a majority of the cases. e2-e1 gene based characterization revealed indian subcontinent sublineage strains possessing aedes aegypti mosquito-adaptive mutations e1: k211e and e2:v264a, with the 211 site positively selected. novel mutational sites e1: k16e/q, e1: k132q/t, e1: s355t, e2: c19r and e2:s185y could be associated with epitopes or virulence de ...201830073419
a low-cost, battery-powered acoustic trap for surveilling male aedes aegypti during rear-and-release operations.the aedes aegypti mosquito is a primary vector of several serious arboviruses throughout the world and is therefore of great concern to many public health organizations. with vector control methodology pivoting towards rearing and releasing large numbers of genetically modified, sterilized, or wolbachia-infected male mosquitoes to control vector populations, economical surveillance methods for release tracking becomes increasingly necessary. previous work has identified that male ae. aegypti are ...201830071091
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
small females prefer small males: size assortative mating in aedes aegypti mosquitoes.with aedes aegypti mosquitoes now being released in field programmes aimed at disease suppression, there is interest in identifying factors influencing the mating and invasion success of released mosquitoes. one factor that can increase release success is size: released males may benefit competitively from being larger than their field counterparts. however, there could be a risk in releasing only large males if small field females avoid these males and instead prefer small males. here we invest ...201830068363
a gustatory receptor used for rapid detection of tyrophagus putrescentiae in fungi hosts.the storage mite, tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in china. storage mites are tiny and difficult to observe, especially when they occur in fungi composts. in this study, one gustatory receptor protein (tputgr1) was identified from the transcriptome of t. putrescentiae. phylogenetic analysis of grs families from 10 arthropod species revealed that tputgr1 had high homology with the sccagr1 of sarcoptes scabiei and turtgr1-2 of tetran ...201830061635
juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes.juvenile hormone (jh) regulates many aspects of insect development and reproduction. in some processes, jh plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20e). in aedes aegypti mosquitoes, jh prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20e after blood ingestion. the molecular basis of this competence is still not well understood. here, we report that jh regulates pre-mrna splicing of the taiman gene, ...201830061397
comparing the effectiveness of different strains of wolbachia for controlling chikungunya, dengue fever, and zika.once aedes aegypti and aedes albopictus mosquitoes that spread chikungunya virus, dengue virus, and zika virus are infected with wolbachia, they have reduced egg laying rates, reduced transmission abilities, and shorter lifespans. since most infected mosquitoes are only infectious in the last few days of their lives, shortening a mosquito's lifespan by a day or two can greatly reduce their abilities to spread mosquito-borne viral diseases, such as chikungunya, dengue fever, and zika. we develope ...201830059498
modeling the importation and local transmission of vector-borne diseases in florida: the case of zika outbreak in 2016.chikungunya, dengue, and zika viruses are all transmitted by aedes aegypti and aedes albopictus mosquito species, had been imported to florida and caused local outbreaks. we propose a deterministic model to study the importation and local transmission of these mosquito-borne diseases. the purpose is to model and mimic the importation of these viruses to florida via travelers, local infections in domestic mosquitoes by imported travelers, and finally non-travel related transmissions to local huma ...201830053386
modifying the biogents sentinel trap to increase the longevity of captured aedes aegypti.aedes aegypti (l.) (diptera: culicidae) is a vector of viruses causing dengue, zika, chikungunya, and yellow fever and subsequently pose a significant global threat to public health. while sampling live mosquitoes is useful for surveillance purposes, most traps targeting aedes kill captured mosquitoes. the biogents sentinel (bgs) trap, the gold standard for capturing ae. aegypti, is one such trap. in our study, we modified the bgs trap to increase the survival of captured ae. aegypti by replacin ...201830053026
experimental study of the susceptibility of a european aedes albopictus strain to dengue virus under a simulated mediterranean temperature regime.dengue virus (denv) has re-emerged in europe driven by the geographic expansion of the mosquito species aedes albopictus and aedes aegypti (diptera: culicidae) and the introduction of the virus by viraemic travellers. in the present study, the vector competence (vc) of ae. albopictus collected in catalonia (northeast spain) was evaluated for two different denv strains, denv-1 and denv-2, the serotypes responsible for all outbreaks of dengue that have occurred in europe. mosquitoes were reared un ...201830051490
larvicidal effectiveness of azolla pinnata against aedes aegypti (diptera: culicidae) with its effects on larval morphology and visualization of behavioural response.dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. aedes aegypti (l.) (diptera: culicidae) is a primary vector for dengue viral infections for humans. current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. the extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and ...201830050688
the impact of the age of first blood meal and zika virus infection on aedes aegypti egg production and longevity.the impact of senescence and pathogen infection on aedes aegypti life-history traits remains poorly understood. this laboratory study focused on the impact of zika virus (zikv) infection and the age of first blood intake on blood meal and clutch sizes, and more importantly on the egg production ratio per μl of blood. three groups of zikv-infected and uninfected ae. aegypti females that received their first blood meal at 7 (young feeders), 14 (mature feeders) and 21 days old (old feeders) were mo ...201830048481
characterization of dengue virus in aedes aegypti and aedes albopictus spp. of mosquitoes: a study in khyber pakhtunkhwa, pakistan.dengue is a vector-borne disease caused by dengue virus. according to the recent report of cdc that one-third population of the world are at high risk with dengue fever. the prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. aedes mosquitoes was reported as the main cause of transmission of dengue virus. so the current study was planned to characterize the virus in aedes mosquitoes collected from different area of pakistan. in current inv ...201830046621
specific human antibody responses to aedes aegypti and aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the pacific.aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. in french polynesia, aedes aegypti is the main vector of dengue, chikungunya and zika, and aedes polynesiensis the primary vector of bancroftian filariasis and a secondary vector of arboviruses. tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. a promising approach to quantify the human-vector contact relies ...201830040826
is autumn the key for dengue epidemics in non endemic regions? the case of argentina.dengue is a major and rapidly increasing public health problem. in argentina, the southern extreme of its distribution in the americas, epidemic transmission takes place during the warm season. since its re-emergence in 1998 two major outbreaks have occurred, the biggest during 2016. to identify the environmental factors that trigger epidemic events, we analyzed the occurrence and magnitude of dengue outbreaks in time and space at different scales in association with climatic, geographic and dem ...201830038860
the effect of skitosnack, an artificial blood meal replacement, on aedes aegypti life history traits and gut microbiota.public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. all target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. vertebrate blood, however, is costly, with a short shelf life. to overcome these constraints, we have developed skitosnack, an artificial blood meal replacement for the mosquito aedes aegypti, the vector of dengue, zika and chikungunya virus. skitosnack contains bovine ...201830038361
classification of containers with aedes aegypti pupae using a neural networks model.this paper discusses the presence of aedes aegypti pupae in different types of containers considering: volume, ph of the container, among other variables.201830036370
acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: an attracticide of aedes aegypti.combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. there is a need for toxicological evaluation before its introduction. the present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass.201830033812
cgti, a novel thermostable kunitz trypsin-inhibitor purified from cassia grandis seeds: purification, characterization and termiticidal activity.cassia grandis trypsin inhibitor (cgti) is a novel plant serine proteinase inhibitor. this study sets out to purify a thermostable inhibitor from the seeds of cassia grandis and to provide biochemical information about a novel peptide belonging to the kunitz family. moreover, toxicity assays against artemia, aedes aegypti larvae-l4 and nasutitermes corniger are evaluated. the purification process was performed using acetone precipitation, trypsin-sepharose-cl4b and superdex-g75. the inhibitor sh ...201830030073
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
impedimetric immunosensor for dengue diagnosis using graphite screen-printed electrodes coated with poly(4-aminophenylacetic acid).dengue is an infectious viral disease transmitted by the aedes aegypti mosquito, the control of which is complex. in addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (dv) has drawn increasing attention. the present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (spe) functionalized with polymer films derived from 4- ...201830182296
dengue virus infection changes aedes aegypti oviposition olfactory preferences.aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory preferences and are capable of olfactory learning especially when seeking their required environmental conditions to lay their eggs. in this study, we showed that semiochemical conditions during aedes aegypti larval rearing affected future female choice for oviposition: water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole reared mosquitoes preferred skatole sites. using two in ...201830181545
a conceptual model for optimizing vaccine coverage to reduce vector-borne infections in the presence of antibody-dependent enhancement.many vector-borne diseases co-circulate, as the viruses from the same family are also transmitted by the same vector species. for example, zika and dengue viruses belong to the same flavivirus family and are primarily transmitted by a common mosquito species aedes aegypti. zika outbreaks have also commonly occurred in dengue-endemic areas, and co-circulation and co-infection of both viruses have been reported. as recent immunological cross-reactivity studies have confirmed that convalescent plas ...201830173664
demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of aedes aegypti.aedes aegypti is the primary mosquito vector of dengue viruses (denv; serotypes 1-4). human-mosquito transmission cycles maintain denv during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. aedes mosquitoes can transmit denv within the vector population through two alternate routes: vertical and venereal transmission (vt and vnt, respectively). we tested the efficiency of vt and vnt in a genetically diverse laboratory (gd ...201830169505
yellow fever in africa and the americas: a historical and epidemiological perspective.yellow fever was transported during the slave trade in the 15th and 16th centuries from africa to the americas where the virus encountered favorable ecological conditions that allowed creation of a sustainable sylvatic cycle. despite effective vector control and immunization programs for nearly a century, yellow fever epidemics reemerged in many latin american countries, particularly brazil. the emergence or reemergence of vector-borne diseases encompasses many intricate factors. yellow fever ou ...201830158957
local and regional dynamics of chikungunya virus transmission in colombia: the role of mismatched spatial heterogeneity.mathematical models of transmission dynamics are routinely fitted to epidemiological time series, which must inevitably be aggregated at some spatial scale. weekly case reports of chikungunya have been made available nationally for numerous countries in the western hemisphere since late 2013, and numerous models have made use of this data set for forecasting and inferential purposes. motivated by an abundance of literature suggesting that the transmission of this mosquito-borne pathogen is local ...201830157921
sublethal effects of a vapour-active pyrethroid, transfluthrin, on aedes aegypti and ae. albopictus (diptera: culicidae) fecundity and oviposition behaviour.it is assumed that mosquitoes surviving exposure to spatial repellents when attempting to bite a host will not have significant adverse impacts on their downstream biology. therefore, a critical knowledge gap is understanding the extent to which sublethal exposure to volatile pyrethroids may damage the performance of mosquitoes that survive exposure to vapour-active pyrethroids. to address this, laboratory-reared aedes aegypti (l.) and ae. albopictus (skuse) were exposed to one of three subletha ...201830157907
zika infection decreases aedes aegypti locomotor activity but does not influence egg production or viability.zika has emerged as a new public health threat after the explosive epidemic in brazil in 2015. it is an arbovirus transmitted mainly by aedes aegypti mosquitoes. the knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.201830156598
oviposition deterrent efficacy and characteristics of a botanical natural product, ocimum gratissimum (l.) oil-alginate beads, against aedes aegypti (l.).this study was aimed at investigating the oviposition deterrent activity of ocimum gratissimum (l.) essential oil (o. gratissimum oil) and its product, ocimum gratissimum (l.)- alginate beads (beads), against aedes aegypti (ae. aegypti) mosquitoes. chemical analysis of o. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and z-β-ocimene (14.95 %) as major constituents. good characteristics of beads were obtained by th ...201830154681
forced salivation as a method to analyze vector competence of mosquitoes.vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. viable virus particles are transmitted during a blood meal via the saliva of an infected mosquito. forced salivation assays allow determining the vector potential on the basis of single mosquitoes, avoiding the use of animal experiments. the method is suitable to analyze a large number of mosquitoes in one experiment within a short period of time. forced saliva ...201830148488
qsar modelling of synergists to increase the efficacy of deltamethrin against pyrethroid-resistant aedes aegypti mosquitoes$.space spraying of deltamethrin allows the control of adult aedes (stegomyia) aegypti mosquitoes. unfortunately, many vector control programs are threatened by the development of resistances that decrease the efficacy of this adulticide. faced with this situation, we can either try to use another insecticide presenting a different mechanism of action or find a strategy that brings back the efficacy of the insecticide at a satisfying level to pursue its use in vector control. restoration of the ef ...201830141356
transcriptomic analysis of aedes aegypti in response to mosquitocidal bacillus thuringiensis llp29 toxin.globally, aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. to identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and bacillus thuringiensis (bt) toxin (llp29 proteins)-treated groups. next-generation sequencing technology was used to sequence the midgut transcriptome of a. aegypt ...201830140020
potential of aedes aegypti populations in madeira island to transmit dengue and chikungunya viruses.aedes spp. mosquitoes mainly transmit the arboviruses dengue virus (denv) and chikungunya virus (chikv) in urban areas, causing a severe public health problem. in 2012-2013, a major dengue outbreak occurred on madeira island where the mosquito aedes aegypti was the only vector. up to now, the competence of ae. aegypti populations from madeira to transmit denv or chikv remains unknown. this study aimed to assess experimentally the ability of ae. aegypti populations from madeira to transmit these ...201830208974
distribution and breeding sites of aedes aegypti and aedes albopictus in 32 urban/peri-urban districts of mozambique: implication for assessing the risk of arbovirus outbreaks.aedes-borne arboviruses have emerged as an important public health problem worldwide and, in mozambique, the number of cases and its geographical spread have been growing. however, information on the occurrence, distribution and ecology of aedes aegypti and ae. albopictus mosquitoes remain poorly known in the country.201830208017
mosquito and tick repellency of two anthemis essential oils from saudi arabia.the essential oils (eos) of anthemis melampodina (am) and anthemis scrobicularis (as) (asteraceae) were extracted from the aerial parts of the plants by hydrodistillation, and their chemical compositions were analyzed using gc-fid and gc-ms. fifty-six components representing 85.5% of the oil composition of anthemis melampdina were identified, and the major components were α-pinene (17.1%) and β-eudesmol (13.8%). forty-one components representing 86% of the oil composition of anthemis scrobicular ...201830202228
the effect of permethrin resistance on aedes aegypti transcriptome following ingestion of zika virus infected blood.aedes aegypti (l.) is the primary vector of many emerging arboviruses. insecticide resistance among mosquito populations is a consequence of the application of insecticides for mosquito control. we used rna-sequencing to compare transcriptomes between permethrin resistant and susceptible strains of florida ae. aegypti in response to zika virus infection. a total of 2459 transcripts were expressed at significantly different levels between resistant and susceptible ae. aegypti. gene ontology analy ...201830200481
quiescence in aedes aegypti: interpopulation differences contribute to population dynamics and vectorial capacity.the strategy of aedes aegypti to prolong embryonic viability by quiescence has severe implications for geographic expansion and maintenance of mosquito populations in areas under control measures. we evaluated the effects of quiescence on biological parameters directly or indirectly associated with population dynamics and vectorial capacity in populations of this mosquito species from two brazilian municipalities characterized as dengue, chikungunya, and zika transmission areas. egg viability, i ...201830200417
nutritional quality during development alters insulin-like peptides' expression and physiology of the adult yellow fever mosquito, aedes aegypti.mosquitoes have distinct developmental and adult life history, and the vectorial capacity of females has been shown to be affected by the larval nutritional environment. however, little is known about the effect of developmental nutrition on insulin-signaling and nutrient storage. in this study, we used aedes aegypti, the yellow fever mosquito, to determine whether larval nutrition affects insulin gene expression. we also determined the traits regulated by insulin signaling, such as stored-nutri ...201830200185
evaluation of eosin-methylene blue as a photosensitizer for larval control of aedes aegypti by a photodynamic process.aedes aegypti (ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and zika. several strategies have been applied to avoid ae. aegypti proliferation by using environmental management, biological, and chemical approaches. however, the development of new methods for effective control of the insect vector population is still needed. photodynamic control is an alternative way to control the vector population by using a physical appr ...201830200177
multiple insecticide resistance mechanisms in primary dengue vector, aedes aegypti (linn.) from dengue endemic districts of sub-himalayan west bengal, india.mosquitoes belonging to genus aedes are the prime vectors of several arboviral diseases such as dengue, zika and chikungunya worldwide. every year numerous cases of dengue infections occur throughout the world, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches.201830199543
plant-derived compounds regulate formation of the insect juvenile hormone receptor complex.insect growth regulators (igrs) are attractive pest control agents due to their high target specificity and relative safety to the environment. recently, plants have been shown to synthesize igrs that affect the insect juvenile hormone (jh) as a part of their defense mechanisms. using a yeast two-hybrid system transformed with the aedes aegypti jh receptor as a reporter system, we identified several jh agonists (jhas) and antagonists (jhans) causing retardation in the ovarian development of fema ...201830195384
extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in aedes aegypti mosquitoes.dengue virus (denv), an arthropod-borne ("arbovirus") virus, causes a range of human maladies ranging from self-limiting dengue fever to the life-threatening dengue shock syndrome and proliferates well in two different taxa of the animal kingdom, mosquitoes and primates. mosquitoes and primates show taxonomic group-specific intolerance to certain codon pairs when expressing their genes by translation. this is called "codon pair bias". by necessity, dengue viruses evolved to delicately balance th ...201830192757
differential proteome analysis of chikungunya virus and dengue virus coinfection in aedes mosquitoes.chikungunya virus (chikv) and dengue virus (denv) are important arboviruses transmitted by aedes mosquitoes. these viruses are known to coexist within the same vector and coinfect the same host. although information is available on the mechanism of replication of chikv and denv when present independently in a vector, reports are lacking on the dynamics of virus-vector interactions when these viruses coexist in a mosquito. the current study attempts to understand the perturbations in the proteome ...201830192139
recent advances in threshold-dependent gene drives for mosquitoes.mosquito-borne diseases, such as malaria, dengue and chikungunya, cause morbidity and mortality around the world. recent advances in gene drives have produced control methods that could theoretically modify all populations of a disease vector, from a single release, making whole species less able to transmit pathogens. this ability has caused both excitement, at the prospect of global eradication of mosquito-borne diseases, and concern around safeguards. drive mechanisms that require individuals ...201830190331
evaluation of (-)-borneol derivatives against the zika vector, aedes aegypti and a non-target species, artemia sp.zika, dengue, and chikungunya are vector-borne diseases of pronounced concern transmitted by the mosquito aedes aegypti linn. (diptera: culicidae). the most important method to avoid outbreaks is to control mosquito spreading by the employment of insecticides and larvicides. failure to control mosquito dispersal is mostly accounted to ae. aegypti resistance to currently available larvicides and insecticides, encouraging the development of novel pesticides. in addition, the excessive use of larvi ...201830187415
knowledge, practices and entomological aspects of dengue in medellín, colombia: a comparative study of neighborhoods with high and low incidenceintroduction: dengue, mainly transmitted by aedes aegypti, is a very important viral disease in terms of public health. colombia is an endemoepidemic country for dengue and, in cities like medellín, there are neighborhoods with high and low incidence. the disease dynamics in the neighborhoods might be determined by differences in the knowledge, practices and entomological aspects of the vector among the communities.objective: to identify the knowledge and practices of residents of neighborhoods ...201830184371
novel larvicide tablets of bacillus thuringiensis var. israelensis: assessment of larvicidal effect on aedes aegypti (diptera: culicidae) in colombia.aedes (stegomyia) aegypti is the vector for dengue, chikungunya, and zika arboviruses. bti-cecif is a bioinsecticide designed and developed in the form of a solid tablet for the control of this vector. it contains bacillus thuringiensis var. israelensis (bti) serotype h-14.201830184370
exposure and sensitization to insects in allergic patients in the tropicsintroduction: mites are an important source of allergens in the tropics. other potential sources of allergens prevalent in the region such as insects have been poorly studied.objective: to determine the relationship between exposure and allergic sensitization to cockroaches, mosquitos, ants and the interaction with mite sensitization.materials and methods: we included patients with allergy tests for blatella germanica, aedes aegypti, solenopsis invicta, blomia tropicalis, dermatophagoides farina ...201830184366
genetic variability of aedes aegypti in the department of sucre, colombia, by analysis of the nucleotide sequence of the mitochondrial nd4 geneintroduction. aedes aegypti is the most important mosquito species in america for the transmission of viruses of dengue, zika, chikungunya and yellow fever. ecological factors as well as chemical controls can affect the genetic composition of ae. aegypti populations, which is why its genetic characterization is necessary.objective. to determine the genetic variability of ae. aegypti populations in four municipalities of sucre department, colombia.materials and methods. larvae of ae. aegypti, col ...201830184356
evaluation of standard field and laboratory methods to compare protection times of the topical repellents pmd and deet.mosquitoes are important vectors of pathogens, and travellers to disease endemic countries are advised to avoid bites by applying topical repellents. topical repellents are typically tested either in the arm-in-cage (aic) test under laboratory conditions or in the field, but not often under both conditions. we, therefore, investigated how two topical repellents, 15% para-menthane-3,8-diol (pmd) and 15% n,n-diethyl-3-methylbenzamide (deet) compare against each other both in the aic test against t ...201830135603
experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant aedes aegypti.the extensive reliance on insecticides to control aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and zika has fueled the emergence of widespread resistance to insecticides. mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide applications for vector control is often hypothesized to be due to household use of commercial insecticide products. we experimentally quantified phenotypic and genotypic responses ...201830135460
differential transmission of antiviral drug-resistant chikungunya viruses by aedes mosquitoes.the chikungunya virus (chikv) is transmitted by female aedes aegypti and aedes albopictus mosquitoes, mostly present in (sub)tropical regions. no antivirals are available to treat chikv infections. if antiviral drugs are proven efficient to treat chikv-infected patients, it will be pivotal to determine whether drug-resistant viruses can be transmitted from one human to another by their mosquito vectors. we orally infected aedes aegypti mosquitoes with a blood meal containing wild-type or drug-re ...201830135220
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