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rotations of bahiagrass and castorbean with peanut for the management of meloidogyne arenaria.the relative value of 'hale' castorbean (ricinus communis) and 'pensacola' bahiagrass (paspalum notatum) as rotational crops for the management of meloidogyne arenaria and southern blight (sclerotium rolfsii) in 'florunner' peanut (arachis hypogaea) production was studied for 3 years in a field experiment in southeast alabama. peanut following 2 years of castorbean (c-c-p) yielded 43% higher than monocultured peanut without nematicide. at-plant application of aldicarb (30.5 g a.i./100 m row in a ...199119283180
rotations of soybean with tropical corn and sorghum for the management of nematodes.the relative efficacy of rotations of soybean with sorghum and tropical corn for nematode management was studied for 2 years in a field infested with root-knot (meloidogyne arenaria) and soybean cyst (heterodera glycines, race 14) nematodes. corn, sorghum, and soybean cv. kirby were planted in 1989, and in 1990 the same areas were planted with seven soybean cultivars with and without at-plant application ofaldicarb. corn and sorghum did not support h. glycines, but significant juvenile populatio ...199119283181
characterisation of potential adhesins of the bacterium pasteuria penetrans, and of putative receptors on the cuticle of meloidogyne incognita, a nematode host.pasteuria penetrans spores were fragmented by glass bead vortexing, producing exosporial membranes and spore fragments, which consisted of fibre bundles. both exosporia and spore fragments are capable of host-specific attachment to the cuticle of meloidogyne incognita, a root-knot nematode host. putative m. incognita receptors appear to be soluble in beta-mercaptoethanol (bme) but not sds, and are also sensitive to tryptic digestion and deglycosylation by endoglycosidase f. polyclonal antibodies ...19911808209
isolation and characterization of a tomato acid phosphatase complementary dna associated with nematode resistance.the tomato (lycopersicon esculentum) acid phosphatase-1 (apase-1(1), ec 3.1.3.2) isozyme variant, genetically linked to the root-knot nematode resistance locus (mi) on chromosome 6, has been purified by a rapid procedure from tomato cell suspension cultures. peptide fragments of the purified enzyme were generated from trypsin and lys-c endoprotease digests and separated by reverse-phase high-performance liquid chromatography. amino acid sequences derived from the purified peptide fragments repre ...199116668572
high resolution rflp map around the root knot nematode resistance gene (mi) in tomato.in the 1940's the root-knot nematode resistance gene (mi) was introgressed into the cultivated tomato from the wild species, l. peruvianum, and today it provides the only form of genetic resistance against this pathogen. we report here the construction of a high resolution rflp map around the mi gene that may aid in the future cloning of this gene via chromosome walking. the map covers the most distal nine map units of chromosome 6 and contains the mi gene, nine rflp markers, and one isozyme mar ...199124213330
rflp markers linked to the root knot nematode resistance gene mi in tomato.the mi gene originating from the wild tomato species lycopersicon peruvianum confers resistance to all major root knot nematodes (meloidogyne spp.). this single dominant gene is located on chromosome 6 and is very closely linked to the acid phosphatase-1 (aps-1) locus. resistance to nematodes has been introgressed into various cultivars of the cultivated tomato (l. esculentum), in many cultivars along with the linked l. peruvianum aps-1 (1) allele. by using a pair of nearly isogenic lines differ ...199124221383
repeated sequence sets in mitochondrial dna molecules of root knot nematodes (meloidogyne): nucleotide sequences, genome location and potential for host-race identification.within a 7 kb segment of the mtdna molecule of the root knot nematode, meloidogyne javanica, that lacks standard mitochondrial genes, are three sets of strictly tandemly arranged, direct repeat sequences: approximately 36 copies of a 102 ntp sequence that contains a taqi site; 11 copies of a 63 ntp sequence, and 5 copies of an 8 ntp sequence. the 7 kb repeat-containing segment is bounded by putative trnaasp and trnaf-met genes and the arrangement of sequences within this segment is: the trnaasp ...19912027769
meloidogyne lusitanica n. sp. (nematoda: meloidogynidae), a root-knot nematode parasitizing olive tree (olea europaea l.).a root-knot nematode from portugal, meloidogyne lusitanica n. sp., is described and illustrated from specimens obtained from olive trees (olea europaea l.). females of the new species have a characteristic perineal pattern with medium to high trapezoidal dorsal arch with distinct punctuations in the tail terminus area. the excretory pore is located posterior to the stylet, about 1.5-2.5 stylet lengths from the anterior end. the stylet is 17.1 mum long with pear-shaped knobs. males have a rounded ...199119283115
acid phosphatase-1(1), a tightly linked molecular marker for root-knot nematode resistance in tomato: from protein to gene, using pcr and degenerate primers containing deoxyinosine.with a view to cloning the root-knot nematode resistance gene mi in tomato by chromosome walking, we have developed a molecular probe for the tightly linked acid phosphatase-1 (aps-1) locus. the acid phosphatase-1 allozyme (aps-1(1], encoded by the aps-1(1) allele originating from lycopersicon peruvianum, was purified to apparent homogeneity from tomato roots and suspension cells. microsequencing of cnbr and tryptic peptides generated from aps-1(1) provided a partial amino acid sequence, which a ...19911651125
invasion of tomato roots and reproduction of meloidogyne incognita as affected by raw sewage sludge.the antagonistic effects of raw sewage sludge on infection of tomato by meloidogyne incognita were tested in greenhouse pot experiments. sludge was mixed with the soil or added on its surface before and after inoculation of tomato plants with nematode eggs. juvenile penetration was determined 1 and 10 days after inoculation, and 6 weeks later root systems were assessed for nematode reproduction. fewer juveniles penetrated roots in pots with sludge added to the soil than in unamended control pots ...199119283192
response of tomato cultigens to meloidogyne javanica and races of meloidogyne incognita.thirty-six cultigens of tomato were evaluated for resistance against meloidogyne javanica and four races of m. incognita with standards and parameters adopted by the international meloidogyne project. most cultigens were susceptible to the nematodes, including some that were previously reported to be resistant to these nematodes. ten accessions, namely pusa-120, calmart vfn, panjab 6.nr-7, ec173898 (72t6), ec173897 (cal-mart), ec173896 (kewalo), cln363bcf-167-1-0, cln363bcf-190-1-0, cln363bcf-34 ...199119283169
interaction of meloidogyne incognita and water stress in two cotton cultivars.a series of controlled-environment experiments were conducted to elucidate the effects of meloidogyne incognita on host physiology and plant-water relations of two cotton (gossypium hirsutum) cultivars that differed in their susceptibility to nematode infection. inoculation of m. incognita-resistant cultivar auburn 634 did not affect growth, stomatal resistance, or components of plant-water potential relative to uninoculated controls. however, nematode infection of the susceptible cultivar stone ...199119283156
evidence against amplification of four genes in giant cells induced by meloidogyne incognita.giant-cell dna was isolated from pea (pisum sativum) inoculated with meloidogyne incognita and used in slot blots to test for selective sequence amplification. four sequences representing low (ribulose 1,5-bisphosphate carboxylase and actin), mid-level (histone 3), and highly repetitive (large ribosomal repeat) sequence dna were used as probes. known amounts of root-tip dna and giant-cell dna were blotted onto hybridization membranes and probed. the signal strength on autoradiographs containing ...199119283148
interaction of meloidogyne javanica with different races of meloidogyne incognita.the interspecific interactions of meloidogyne javanica with races 1, 2, 3, and 4 of m. incognita on tomato were determined. impacts of the interactions on fecundity and morphometrics of females were also examined. mutually inhibitory interactions occurred between m. javanica and the races of m. incognita, but the negative interactions did not reflect in plant growth. numbers of root galls, egg masses, mature females, total population, fecundity, and reproduction factor declined in concomitant tr ...199119283130
modification of resistance expression of phaseolus vulgaris to meloidogyne incognita by elevated soil temperatures.the effect of temperature on the reaction of susceptible (canario divex) and resistant (a 211) bean pure lines to meloidogyne incognita was studied with soil temperature tanks housed in a growth chamber at 22 or 24 c. soil temperature remained constant at 16, 22, 24, 26, 30, or 32 c in several trials. bean line a 211 was resistant at 16 and 22 c but was susceptible at 24 c and above. resistance to root-knot nematode reproduction was affected by a lower temperature (24 c) than was resistance to r ...199119283110
effects of incorporation method of ethoprop and addition of aldicarb on potato tuber infection by meloidogyne hapla.the efficacy of controlling meloidogyne hapla on potato with water incorporation of ethoprop was compared to physical incorporation before planting. the standard practice of aldicarb application for insect control was also evaluated for m. hapla suppression with and without ethoprop. physical incorporation before planting by rototilling or discing reduced (p </= 0.05) tuber infection. postplant water incorporation of ethoprop was not as effective as physical incorporation of ethoprop or postplan ...199119283186
further studies on the role of polyploidy in the evolution of meloidogyne.two tetraploid isolates of meloidogyne hapla, 86p and e289p, with haploid chromosome numbers of 34 and 28, respectively, were studied cytogenetically and biologically in relation to the diploid populations, 86-va (n = 17) and e289-taiwan (n = 14), from which they had been originally isolated. both isolates were quite stable, converting to diploidy at the low rate of about 2.5%. the tetraploid isolate 86p maintained itself in competition with its diploid counterpart in mixed cultures, although an ...199119283121
efficacy of ethoprop on meloidogyne hapla and m. chitwoodi and enhanced biodegradation in soil.responses of egg masses, free eggs, and second-stage juveniles (j2) ofmeloidogyne hapla and m. chitwoodi to ethoprop were evaluated. the results indicated that j2 were the most sensitive, followed by free eggs and egg masses. in general, m. chitwoodi was more susceptible to ethoprop than m. hapla. ethoprop at 7.2 mug a.i./g soil protected tomato roots from upward migrating m. chitwoodi for 5 weeks. the zone of protection was extended to 10 and 20 cm below the root zone when 3.6 and 7.2 cm water ...199119283141
population dynamics of meloidogyne chitwoodi on russet burbank potatoes in relation to degree-day accumulation.population dynamics of meloidogyne chitwoodi were studied for 2 years in a commercial potato field and microplots. annual second-stage juvenile (j2) densities peaked at harvest in mid-fall, declined through the winter, and were lowest in early summer. in the field and in one microplot study, population increase displayed trimodal patterns during the 1984 and 1985 seasons. overwintering nematodes produced egg masses on roots by 600-800 degree-days base 5 c (dd) after planting. second-generation a ...199119283128
suppression of root-knot nematode populations with selected rapeseed cultivars as green manure.meloidogyne chitwoodi races 1 and 2 and m. hapla reproduced on 12 cultivars of brassica napus and two cultivars of b. campestris. the mean reproductive factors (rf), rf = pf at 55 days / 5,000, for the three nematodes were 8.3, 2.2, and 14.3, respectively. all three nematodes reproduced more efficiently (p < 0.05) on b. campestris than on b. napus. amending m. chitwoodi-infested soil in plastic bags with chopped shoots of jupiter rapeseed reduced the nematode population more (p < 0.05) than amen ...199119283108
seasonal migration of meloidogyne chitwoodi and its role in potato production.seasonal vertical migration of meloidogyne chitwoodi through soil and its impact on potato production in washington and oregon was studied. nematode eggs and second-stage juveniles (j2) were placed at various depths (0-180 cm) in tubes filled with soil and buried vertically or in holes dug in potato fields. tubes were removed at intervals over a 12-month period and soil was bioassayed on tomato roots. upward migration began in the spring after water had percolated through the tubes. nematodes we ...199119283107
growth of carrot and tomato from oxamyl-coated seed and control of meloidogyne hapla.oxamyl was coated on carrot (daucus carota l. cv. spartan fancy-80) and tomato (lycopersicon esculentum mill. cv. glamour) seeds with a polymer sticker for the control of meloidogyne hapla. the sticker diluted in water 1:1 delayed carrot seedling emergence. oxamyl at 40 mg/ml in a 1:5 dilution of sticker lowered the rate of carrot seedling emergence until day 13 and plant growth until day 28. oxamyl at 20 or 40 mg/ml in a 1:5 dilution of sticker on carrot seeds planted in m. hapla-infested muck ...199019287706
identification of single meloidogyne juveniles by polymerase chain reaction amplification of mitochondrial dna.polymerase chain reaction (pcr) was used to amplify a specific 1.8-kb sequence of mitochondrial dna from single juveniles and eggs from 17 populations of meloidogyne incognita, m. hapla, m. javanica, and m. arenaria. approximately 2 mug amplified product were produced per reaction. restriction digestion of the amplified product with hinfi permitted discrimination of clonal lineages of the four species. meloidogyne javanica, however, could not be separated from m. hapla by the enzymes used in the ...199019287752
effects of planting date, small grain crop destruction, fallow, and soil temperature on the management of meloidogyne incognita.the effects of planting date, rye (secale cereale cv. wren abruzzi) and wheat (triticura aestivum cv. coker 797), crop destruction, fallow, and soil temperature on managing meloidogyne incognita race 1 were determined in a 2-year study. more m. incognita juveniles (j2) and egg-producing adults were found in roots of rye planted 1 october than in roots of rye planted 1 november and wheat planted 1 november and 1 december. numbers of m. incognita adults with and without egg masses were near or bel ...199019287731
strong repellency of the root knot nematode,meloidogyne incognita by specific inorganic ions.simple inorganic salts of the ions k(+), nh 4 (+) , cs(+), no 3 (-) , and cl(-) are strongly repellent to infective second-stage larvae of the root knot nematode,meloidogyne incognita. some of these salts are known to be beneficial to plant growth. the results suggest a new means of plant protection.199024263720
response of resistant soybean plant introductions to meloidogyne incognita in field microplots.the response of two soybean plant introductions, pi 96354 and pi 417444, highly resistant to meloidogyne incognita, to increasing initial soil population densities (pi) (0, 31, 125, and 500 eggs/100 cm(3) soil) of m. incognita was studied in field microplots for 2 years. the plant introductions were compared to the cultivars forrest, moderately resistant, and bossier, susceptible to m. incognita. averaged across years, the yield suppressions of bossier, forrest, pi 417444, and pi 96354 were 97, ...199019287716
effects of cowpea and maize root leachates on meloidogyne incognita egg hatch. 199019287701
an assessment of progress toward microbial control of plant-parasitic nematodes. 199019287771
effect of temperature on expression of resistance to meloidogyne spp. in common bean (phaseolus vulgaris).the effect of soil temperature on the expression of resistance in several common bean lines carrying resistance to root-knot nematodes (meloidogyne spp.) was studied under controlled temperatures in temperature tank and growth chamber conditions. resistance to m. javanica and m. incognita race 1 in bean lines a315, a328, a445, g1805, and g2618 was stable at 24-30 c. however, there was a significant increase in reproduction of m. javanica on a315, a328, and a445 when temperature was increased fro ...199019287744
optimization of mitochondrial dna-based hybridization assays to diagnostics in soil.nucleic acid hybridization among root-knot nematode mitochondrial dnas can be used to identify several meloidogyne species. research was initiated to optimize mitochondrial dna-based molecular diagnostics for the demanding environments likely to be encountered in field isolates. dna hybridization using reconstituted dna-soil mixtures revealed a loss of assay sensitivity ranging from 34% to 92% with four agronomic soils tested. this problem was alleviated by the addition of exogenously added dna. ...199019287721
morphological comparison of three host races of meloidogyne javanica.a morphological and morphometric comparison using light microscopy and scanning electron microscopy was made of six populations of meloidogyne javanica belonging to three host races (infective on pepper, peanut, or noninfective on both). the variability of certain morphological characters was studied within these populations, and the reliability of these taxonomic traits was evaluated for usefulness in species identification. the most useful diagnostic characters of m. javanica were head and sty ...199019287689
molecular diagnosis of meloidogyne species.genetic variation within nuclear and mitochondrial dna of meloidogyne species and host races has been evaluated for the development of root-knot nematode molecular diagnostics. this review summarizes the distinctive features of several useful dna-based assays for plant-parasitic nematodes, focusing upon the direct application of these procedures for meloidogyne detection, identification, and systematics.199019287685
vertical distribution of plant-parasitic nematodes in sandy soil under soybean.vertical distribution of five plant-parasitic nematodes was examined in two north florida soybean fields in 1987 and 1988. soil samples were collected from 0-15 cm, 15-30 cm, and 30-45 cm deep at each site. soil at the three depths consisted of approximately 96% sand. more than 50% of belonolaimus longicaudatus population densities occurred in the upper 15-cm soil layer at planting, but the species became more evenly distributed through the other depths as the season progressed. criconemella sph ...199019287693
association of verticillium chlamydosporium and paecilomyces lilacinus with root-knot nematode infested soil.population densities of meloidogyne incognita and the nematophagous fungi, paecilomyces lilacinus and verticillium chlamydosporium, were determined in 20 northern california tomato fields over two growing seasons. paecilomyces lilacinus was isolated from three fields, v. chlamydosporium was isolated from one field, and both fungi were isolated from 12 fields. verticillium chlamydosporium numbers were positively correlated with numbers of m. incognita and p. lilacinus. paecilomyces lilacinus numb ...199019287711
meloidogyne morocciensis n. sp. (meloidogyninae), a root-knot nematode from morocco.meloidogyne morocciensis n. sp. is described from specimens parasitic on peach rootstock from morocco. this species exhibits a combination of morphological characters similar to m. arenaria, m. incognita, and m. javanica. the perineal pattern of females is oval to squarish with a moderately high to high dorsal arch, and widely spaced, smooth striae; lateral lines are absent. the stylet, 16.5 mum long, has transversely ovoid, set-off knobs. males have a set-off, annulated head region. the large, ...199019287722
host range and ecology of isolates of pasteuria spp. from the southeastern united states.isolates of pasteuria penetrans were evaluated for ecological characteristics that are important in determining their potential as biological control agents. isolate p-20 survived without loss of its ability to attach to its host nematode in dry, moist, and wet soil and in soil wetted and dried repeatedly for 6 weeks. some spores moved 6.4 cm (the maximum distance tested) downward in soil within 3 days with percolating water. the isolates varied greatly in their attachment to different nematode ...199019287753
repeated sampling to determine the precision of estimating nematode population densities.the first phase of this study involved repeated samplings of five fields using composite samples of 10, 20, 40, and 80 soil cores, to determine the precision of nematode assays. the second phase focused on randomly selecting two and four 2-ha subunits (data on meloidogyne spp.) of 24 fields ranging from 6 to 40 ha and computing the precision of estimated means for these numbers ofsubunits versus the general field mean (based on all 2-ha subunits). average numbers of nematodes from most samples c ...199019287757
spring or fall fumigation for control of meloidogyne spp. on tobacco.tests were conducted in 1987-88 to compare the efficacy of spring or fall fumigant nematicide applications for control ofmeloidogyne arenaria and m. incognita on tobacco. chloropicrin, 1,3-d, methyl isothiocyanate, and a methyl isothiocyanate-l,3-d mixture were applied as row treatments. fenamiphos, fenamiphos + fensulfothion, or ethoprop were applied in the spring as nonfumigant nematicide standards. fumigant nematicides increased yields and reduced galling (p = 0.01) in all four tests. spring ...199019287773
plant-parasitic nematodes and fungi associated with root rot of peas on prince edward island.eight commercial pea fields on prince edward island were sampled in june and july over a 2-year period (1986-87) to determine soil population densities and the incidence of nematodes and fungi associated with root rot of peas. root lesion nematodes (pratylenchus spp.) were the dominant endoparasitic nematodes recovered from roots and soil. low populations of the northern root-knot nematode (meloidogyne hapla) were also present. tylenchorhynchus spp. and paratylenchus spp. were recovered frequent ...199019287779
resistance of maize to meloidogyne arenaria and meloidogyne javanica.a diallel cross of eight maize, zea mays l., inbred lines was analyzed for reaction to two species of root-knot nematodes, meloidogyne arenaria (neal) chitwood and m. javanica (treub) chitwood. egg production following inoculation of f1 hybrid seedlings with nematode eggs was determined in a greenhouse experiment. data were analyzed using griffing's method 4, model i. general combining ability was a significant source of variation in egg production of both m. arenaria and m. javanica; specific c ...199024221114
evaluation of soybean cultivars for production in meloidogyne arenaria race 2-infested soil.field trials with 56 soybean cultivars and breeding lines from public and private sources were conducted from 1986 through 1988 at a site infested with meloidogyne arenaria race 2. differences (p < 0.05) among yields were found each year and yields were negatively correlated (p < 0.01) with root-knot nematode galling. all entries were galled and the highest-yielding entries, 'kirby' and 'coker 6738', were determined to have average yield reductions of 56% and 62%, respectively, when compared wit ...199019287790
resistance in lycopersicon peruvianum to isolates of mi gene-compatible meloidogyne populations.root-knot nematode resistance of f progeny of an intraspecific hybrid (lycopersicon peruvianum var. glandulosum acc. no. 126443 x l. peruvianum acc. no. 270435), l. esculentum cv. piersol (possessing resistance gene mi), and l. esculentum cv. st. pierre (susceptible) was compared. resistance to 1) isolates of two meloidogyne incognita populations artificially selected for parasitism on tomato plants possessing the mi gene, 2) the wild type parent populations, 3) four naturally occurring resistan ...199019287762
effects of root-knot nematodes on areca catechu.no root galling or egg mass formation was noted on betel palm (areca catechu) 3 or 9 months after inoculation with meloidogyne arenaria, m. hapla, m. incognita races 1 and 3, or m. javanica. compared with uninoculated controls, a reduction (p </= 0.05) in fresh root weight was noted after 3 months with m. incognita race 1 but not with other species or races, no differences (p </= 0.05) in root weight between controls and inoculated plants were observed at 9 months, nor were any differences found ...198919287679
crops uncommon to alabama for the management of meloidogyne arenaria in peanut.in a 1987 field study juveniles of meloidogyne arenaria assayed at the time of peanut harvest were almost undetectable in plots planted with american jointvetch (aeschynomene americana), castor bean (ricinus communis), partridge pea (cassia fasiculata), sesame (sesamum indicum), and cotton (gossypium hirsutum), whereas plots with peanut (arachis hypogaea) averaged 120 juveniles/100 cm(3) soil. application of aldicarb in peanut resulted in an average of 27 juveniles/100 cm(3) soil. in 1988 all pl ...198919287678
host suitability of soybean cultivars for meloidogyne incognita and m. arenaria.the suitability of five maturity group (mg) iii and five mg iv soybean, glycine max, cultivars as hosts for meloidogyne incognita and m. arenaria was evaluated in a greenhouse. 'forrest', a mg v cultivar, was used as the standard of comparison for m. incognita resistance. with m. incognita, root-gall and egg-mass indices and reproductive factors for 'asgrow 3307', 'ffr 398', and 'pioneer 9442' were comparable with those found on forrest. meloidogyne arenaria reproduction was lower (p </= 0.05) o ...198919287669
resistance to meloidogyne arenaria in arachis spp. germplasm.field and greenhouse evaluations of 116 wild arachis spp. genotypes demonstrated the presence of resistance to reproduction of the root-knot nematode meloidogyne arenaria race 1. resistance in greenhouse tests was based on test lines having </= 2.5% of the number of eggs per gram of roots as did the susceptible a. hypogaea cv. tamnut 74. in field tests, resistant genotypes were identified on the basis of having lower (p = 0.05) final nematode population densities than did tamnut 74. resistance w ...198919287667
suitability of small grains as hosts of meloidogyne species.seven cultivars of wheat, five of oat, one of rye, and four of barley were tested as hosts for meloidogyne incognita, m. javanica, or m. arenaria under greenhouse conditions where soil temperature ranged from 21 to 34 c. reproduction rates of all nematode species were high on all cultivars, except m. javanica and m. arenaria on 'brooks' and 'florida 501' oat. meloidogyne incognita and m. javanica produced more eggs on roots of 'rutgers' tomato than on cultivars of wheat, oat, rye, or barley.198919287666
effect of carbamate, organophosphate, and avermectin nematicides on oxygen consumption by three meloidogyne spp.second-stage juveniles (i2) of meloidogyne arenaria consumed more oxygen (p </= 0.05) than m. incognita j2, which in turn consumed more than m. javanica j2 (4,820, 4,530, and 3,970 mul per hour per g nematode dryweight, respectively). decrease in oxygen consumption depended on the nematicide used. except for aldicarb, there was no differential sensitivity among the three nematode species. meloidogyne javanica had a greater percentage decrease (p </= 0.05) in oxygen uptake when treated with aldic ...198919287640
screening subterranean clover (trifolium spp.) germplasm for resistance to meloidogyne species.this study was conducted to identify lines of subterranean clover (trifolium spp.) with resistance to meloidogyne arenaria (neal, 1989) chitwood, 1949, race 1; m. incognita (kofoid and white, 1919) chitwood, 1949, race 3; and m. javanica (treub, 1885) chitwood, 1949. a collection of 134 subterranean clover lines was evaluated and all had intermediate to high susceptibility. root galling was negatively correlated with both seed and dry matter yields. soil fumigation significantly reduced the nema ...198919287623
dna complexity of the root-knot nematode (meloidogyne spp.) genome.cot curves derived from renaturation kinetics of sheared denatured dna indicated that the genome of six populations representing the four most common root-knot nematode species (meloidogyne incognita, m. arenaria, m. javanica, and m. hapla) is composed of 20% repetitive and 80% nonrepetitive sequences of dna. cot curves were almost identical, indicating that all populations had a haploid genome of approximately the same size. calculations from an average cot curve gave an estimate of 0.51 x 108 ...198919287606
biological control of meloidogyne hapla on alfalfa and tomato with the fungus meria coniospora.this study was to determine whether arthrobotrys flagrans, a. oligospora, and meria coniospora would control the root-knot nematode meloidogyne hapla on alfalfa and tomato. alfalfa seeds were coated with a fungus-rye powder in 2% cellulose and were planted in infested soil. three-week-old seedlings from seed treated with m. coniospora had 60% and 58% fewer galls in two experiments than did seedlings from untreated seeds. numbers of j2 in the soil were not reduced. plant growth did not improve. w ...198919287596
impact of paecilomyces lilacinus inoculum level and application time on control of meloidogyne incognita on tomato.microplot experiments were conducted to evaluate the effects of inoculum level and time of application of paecilomyces lilacinus on the protection of tomato against meioidogyne incognita. the best protection against m. incognita was attained with 10 and 20 g of fungus-infested wheat kernels per microplot which resulted in a threefold and fourfold increase in tomato yield, respectively, compared with tomato plants treated with this nematode alone. greatest protection against this pathogen was att ...198919287585
survival of paecilomyces lilacinus in selected carriers and related effects on meloidogyne incognita on tomato.laboratory and microplot experiments were conducted to determine the influence of carrier and storage of paecilomyces lilacinus on its survival and related protection of tomato against meloidogyne incognita. spores of p. lilacinus were prepared in five formulations: alginate pellets (pellets), diatomaceous earth granules (granules), wheat grain, soil, and soil plus chitin. fungal viability was high in wheat and granules, intermediate in pellets, and low in soil and chitin-amended soil stored at ...198919287586
efficacy of oxamyl coated on alfalfa seed with a polymer sticker in pratylenchus and meloidogyne infested soils.a polymer sticker was used as a coating in which oxamyl was applied to seeds of alfalfa cultivar saranac for the control of pratylenchus penetrans and meloidogyne hapla. the sticker, diluted 1:1 (sticker:water) to 1:5, delayed seedling emergence during the first 4 days after planting. by day 13, however, emergence from all sticker treatments was comparable to the control. shoot growth of seedlings at day 21 was less than that of the control only from seeds coated with a 1:1 dilution; root growth ...198919287603
monoclonal antibodies to secretory granules in esophageal glands of meloidogyne species.monoclonal antibodies to secretory granules in the dorsal or subventral esophageal glands were generated by injecting balb/c mice with immunogens from preparasitic second-stage juveniles (j2) of meloidogyne incognita. antibodies specific for secretory granules in the j2 subventral esophageal glands or the dorsal gland were identified by indirect immunofluorescence microscopy. only antibodies that reacted with granules in the j2 dorsal gland reacted with the esophageal gland lobe ofm. incognita a ...198919287625
further details and sem observations on meloidogyne marylandi (nematoda: meloidogynidae).specimens of meloidogyne marylandi from bermuda grass and a population from zoysia grass were examined and compared morphologically by light and electron microscopy. the populations probably are conspecific and the differences noted in the zoysia population, mainly those of second-stage juveniles (j2) with shorter tails, are considered normal variations rather than representing another form. scanning electron microscope observations provided additional details of the perineal pattern and head of ...198919287638
effects and dynamics of a nematode community on maize.relationships between nematode density and yield and between final and preplant population levels were examined in small maize plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and a xiphinema sp. plant growth--including stand count, grain yield, stalk weight, and size of young plants--often was inversely correlat ...198919287639
effects and dynamics of a nematode community on soybean.the relationships between densities of all members of a plant-parasitic nematode community and yield of 'davis' soybean and between final and preplant population levels were examined in small plots on sandy soils in north-central florida. plant-parasitic nematodes present in the community included belonolaimus longicaudatus, criconemella sphaerocephala, meloidogyne incognita, paratrichodorus minor, pratylenchus brachyurus, and xiphinema sp. plant growth, including stand count, soybean yield (kg/ ...198919287643
yield relationships and population dynamics of meloidogyne spp. on flue-cured tobacco.the complex nature of nematode-induced diseases of tobacco, the relationships between nematode levels and damage, the methods of determining these relationships, and the evolving nematode communities on tobacco in eastern north carolina are described. crop damage associated with these pathogens varies with nematode race and species, crop cultivar, microflora, and environmental conditions. root-gall indices as well as initial and mid-season numbers of meloidogyne spp, in soil are useful for estim ...198919287654
managing root-knot on tobacco in the southeastern united states.root-knot nematodes suppress yields of flue-cured tobacco an estimated 0.1 to 4.8% annually in the southeastern united states, even though nematode management practices have been widely adopted. although meloidogyne incognita races 1 and 3 have predominated, m. arenaria, m. javanica, and m. incognita races 2 and 4 are increasingly important. seventy-five percent of the flue-cured tobacco hectarage in north carolina and virginia is rotated on 2-year or 3-year intervals. over half of the hectarage ...198919287655
long-term effect of crop rotation on soybean in a field infested with meloidogyne arenaria and heterodera glycines.previous cropping sequence (corn-soybean vs. soybean-soybean) and aldicarb effects on soybean yield and nematode numbers at harvest for soybean cultivars with various combinations of nematode resistance were determined in 1988 in a sandy loam soil infested with meloidogyne arenaria race 2 and heterodera glycines races 3 and 4 at elberta, alabama. yield and nematode numbers differed among cultivars with 'leflore' having the highest yield. aldicarb treatment resulted in increased soybean yield but ...198919287680
composition of high molecular weight excretions-secretions from infective larvae of meloidogyne javanica.infective larvae (j2) of meloidogyne javanica were incubated in distilled water for up to 14 days, and their high molecular weight (> 1,000 daltons) excretions-secretions (es) were isolated and partially characterized. the es consisted of a mixture of proteins, glycoproteins, and proteoglycans or polysaccharides as revealed by differential staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compositional analysis. carbohydrate, with approximately equal amounts of neutral mo ...198919287641
esterase and malate dehydrogenase phenotypes in portuguese populations of meloidogyne species.nonspecific esterases and malate dehydrogenases of 1-5 females from 40 root-knot nematode populations from portugal were analyzed by electrophoresis in 0.4-mm-thick polyacrylamide gels. fourteen major bands of esterase activity were detected, corresponding to 10 distinct phenotypes, meloidogyne javanica and m. hapla had distinct species-specific phenotypes. two phenotypes occurred in m. arenaria. the most variability was found among m. incognita populations. of the remaining two phenotypes, one ...198919287618
genetic control of embryo formation in anther culture of diploid potatoes.a diploid potato clone ah 78/8015.37a with androgenetic ability was crossed with a root-knot nematode resistant diploid clone 381320.23 which did not have this ability.among 19 f1 progenies tested a wide range of continuous variability was found for androgenetic capability. four f1 clones with different level of embryo formation capability were backcrossed to 381320.23 to produce 4 f1bc1 families to further clarify the genetic control of androgenetic capability.from the wide range of continuous ...198924232996
a technique for preparing perineal patterns of root-knot nematodes for scanning electron microscopy. 198919287589
drip irrigation as a delivery system for infestation of field plots with nematodes.a drip irrigation delivery system was used to infest field sites with the plant-parasitic root-knot nematodes, meloidogyne incognita. juvenile or egg inocula passed through the system without blockage of emitters or harm to the nematodes. field sites so infested were available for experimentation. delivery of approximately 5 x 10 to 10 juveniles or 10 to 3 x 10 eggs per emitter through the drip system resulted in heavy root galling of tomatoes planted next to the drip emitters. nematodes feeding ...198919287648
root tissue response of two related soybean cultivars to infection by lectin-treated meloidogyne spp.treatment of second-stage juveniles (j2) of meloidogyne incognita race 1 and m. javanica with soybean agglutinin, concanavalin a, wheat germ agglutinin, lotus tetragonolobus agglutinin, or limax flavus agglutinin or the corresponding competitive sugars for each of these lectins did not alter normal root tissue response of soybean cultivars centennial and pickett 71 to infection by m. incognita race 1 or m. javanica. giant cells were frequently induced in centennial and pickett 71 roots 5 and 20 ...198919287600
preferred temperature of meloidogyne incognita.in laboratory thermal gradients, newly hatched infective juveniles of the plant-parasitic root-knot nematode meloidogyne incognita migrated toward a preferred temperature that was several degrees above the temperature to which they were acclimated. after shifting egg masses to a new temperature, the preferred temperature was reset in less than a day. possible functions of this type of thermotaxis are discussed, including the use of thermal gradients around plant roots to locate hosts and to main ...198919287582
overwintering stages of meloidogyne incognita in vitis vinifera.the overwintering of meloidogyne incognita in and around vitis vinifera cv. french colombard roots was studied in a naturally infested vineyard at the kearney agricultural center, in a growth chamber, in inoculated vines in microplots at the university of california, davis, and in a greenhouse. infected roots were sampled at intervals from onset of vine dormancy until plants accumulated about 800 degree days (dd - base 10 c). embryogenesis within eggs, classified as less than or more than 16 cel ...198919287581
influence of glomus intraradices and soil phosphorus on meloidogyne incognita infecting cucumis melo.the interaction among glomus intraradices, meloidogyne incognita, and cantaloupe was studied at three soil phosphorus (p) levels in a greenhouse. all plants grew poorly in soil not amended with p, regardless of mycorrhizal or nematode status. in soil amended with 50 mug p /g soil, m. incognita suppressed the growth of nonmycorrhizal plants by 84%. in contrast, growth of mycorrhizal plants inoculated with m. incognita was retarded by only 21%. a similar trend occurred in plants grown in soil with ...198919287577
resistance to meloidogyne spp. in allohexaploid wheat derived from triticum turgidum and aegilops squarrosa.expression of resistance to meloidogyne incognita and m. javanica from aegilops squarrosa was studied in a synthetic allohexaploid produced from triticum turgidum var. durum cv. produra and ae. squarrosa g 3489. the reproductive rate of different races of m. incognita and m. javanica, expressed in eggs per gram of fresh root, was low (p < 0.05) on the synthetic allohexaploid and the resistant parent, ae. squarrosa g 3489, compared with different bread and durum wheat cultivars. reproduction of r ...198919287575
susceptibility of nevada synthetic xx germplasm to a california race of meloidogyne hapla. 198919287611
pathogenicity of two populations of meloidogyne hapla chitwood on alfalfa and sainfoin.the pathogenicity of two populations of the northern root-knot nematode, meloidogyne hapla chitwood, population 1 (p1) from alfalfa and population 2 (p2) from sainfoin, was studied on both alfalfa and sainfoin for 25 weeks. alfalfa and sainfoin plants inoculated with p2 had significantly (p </= 0.05) higher mortality than plants inoculated with p1. plant stands over all weeks for the uninoculated control, p1, and p2 were 90.5, 78.5, and 64.0% for alfalfa and 84.5, 51.0, and 41.0% for sainfoin, r ...198919287580
comparison of fumigant and nonfumigant nematicides for control of meloidogyne chitwoodi on potato.the fumigant 1,3-dichloropropene (1,3-d) effectively controlled meloidogyne chitwoodi on russet burbank potato, solanum tuberosum. there was a maximum of 4% infected and galled tubers from the 1,3-d treatment after 2,028 degree-days with a base temperature of 5 c (dd5). this compared to 66% infected and galled tubers in aldicarb at-plant treated plots. soil temperature, as determined by dd5, and timing of chemical applications affected the nematicidal activity on m. chitwoodi (p < 0.05). aldicar ...198919287664
resistance in triticum and aegilops spp. to meloidogyne chitwoodi.two lines of aegilops squarrosa (g 3489 and g 1279) and triticum cultivars anza, cocorit, produra, chinese spring, nugaines, and a synthetic hexaploid were screened for resistance to meloidogyne chitwoodi. reproduction of m. chitwoodi, expressed as eggs per gram root, was low (p < 0.01) on g 3489 and the synthetic hexaploid. reproduction on all other cultivars tested was high although differences (p < 0.01) existed among them.198919287662
pathological reaction of crested wheatgrass cultivars to four meloidogyne chitwoodi populations.meloidogyne chitwoodi populations from tulelake, california; ft. hall, idaho; beryl, utah; and prosser, washington, significantly (p < 0.05) reduced dry shoot weights of crested wheatgrass (agropyron cristatum l., gaertn. and a. desertorum, fisch. ex link, schult.) cultivars hycrest, fairway, and nordan in experiments conducted in a greenhouse and growth chamber. shoot growth depression, root galling, and nematode reproduction indices were greatest (p < 0.05) on plants inoculated with 5,000 eggs ...198919287637
host-parasite relationship of carrot cultivars and meloidogyne chitwoodi races and m. hapla.most of the 15 carrot cultivars tested were moderate to good hosts to meloidogyne chitwoodi race 1, whereas all except orlando gold were nonhosts or poor hosts for m. chitwoodi race 2. all carrot cultivars were good hosts for m. hapla. the plant weights of the carrot cultivars red cored chantenay and orlando gold infected with either race of m. chitwoodi were significantly less than uninoculated checks in pots. under field microplot conditions, however, detrimental effects on quality were rarely ...198819290254
host tests to differentiate meloidogyne chitwoodi races 1 and 2 and m. hapla.the reproductive factor (r = final egg density at 55 days / 5,000, initial egg density) of meloidogyne chitwoodi race 2 (alfalfa race) on 46 crop cultivars ranged from 0 to 130. the reproductive efficiency of m. chitwoodi race 1 (non-alfalfa race) and m. chitwoodi race 2 was compared on selected crop cultivars. the basic difference between the two races lay in their differential reproduction on thor alfalfa and red cored chantenay carrot. m. chitwoodi race 2 reproduced on alfalfa but not on carr ...198819290239
differential response of thor alfalfa to meloidogyne chitwoodi races and m. hapla.second-stage juveniles (j2) of races 1 and 2 of meloidogyne chiiwoodi and m. hapla readily penetrated roots of thor alfalfa and columbian tomato seedlings; however, few individuals of m. chitwoodi race 1 were able to establish feeding sites and mature on alfalfa. histopathological studies indicate that j2 of race 1 either failed to initiate feeding sites or they caused cell enlargement without typical cell wall thickening. the protoplasm of these cells coagulated, and juveniles of race 1 did not ...198819290231
screening of a granular chelate of metham-zinc for nematicidal activity using citrus and root-knot nematodes.a granular formulation of a chelate of metham-zinc (cmz) which liberates the biocidal methyl isothiocyanate was tested for nematicidal activity on tylenchulus semipenetrans in a jar soil screening and on meloidogyne javanica (greenhouse test) and m. incognita (field test) infecting tomato. comparisons were made with 1,3-d in the jar and pot experiments. the cmz caused only 3.9% mortality of citrus nematode juveniles at 1.0 mug a.i./g soil, but 95.4% mortality at 10.0 mug a.i./g and 100.0% at 100 ...198819290294
characterization of carbohydrates on the surface of second-stage juveniles of meloidogyne spp.fluorescent conjugates of the lectins soybean agglutinin (sba), concanavalin a (con a), wheat germ agglutinin (wga), lotus tetragonolobus agglutinin (lot), and limulus polyphemus agglutinin (lpa) bound primarily to amphidial openings and amphidial secretions of viable, preinfective second-stage juveniles (j2) of meloidogyne incognita races 1 and 3 (mil, mi3) and m. javanica (mj). no substantial difference in fluorescent lectin binding was observed among the populations examined. binding of only ...198819290262
extremely sensitive thermotaxis of the nematode meloidogyne incognita.eggs of the root-knot nematode meloidogyne incognita were acclimated to 23 c. newly hatched second-stage juveniles migrated toward higher temperatures when placed in shallow thermal gradients averaging 23 c. the threshold gradient for this response was below 0.001 c/cm, with a best estimate of 4 x 10 c/cm. calculations of physical limitations on thermotaxis indicate that this sensitivity is well within the limits of what is physically possible.198819290261
growth and energy demand of meloidogyne incognita on susceptible and resistant vitis vinifera cultivars.food (energy) consumption rates ofmeloidogyne incognita were calculated on vitis vinifera cv. french colombard (highly susceptible) and cv. thompson seedless (moderately resistant). one-month-old grape seedlings in styrofoam cups were inoculated with 2,000 or 8,000 m. incognita second-stage juveniles (j2) and maintained at 17.5 degree days (dd - base 10 c)/day until maximum adult female growth and (or) the end of oviposition. at 70 dd intervals, nematode fresh biomass was calculated on the basis ...198819290253
relationship between egg viability and population densities of meloidogyne incognita on cotton.cotton seedlings were inoculated with a range of initial populations (pi) of meloidogyne incognita in greenhouse experiments to test the relationship between nematode population densities and egg viability. in two of three experiments, a significant (p < 0.05) negative linear relationship was detected between percentage of hatch of first generation eggs and log pi. a similar relationship between hatch and root-gall index was observed. in two experiments numbers of eggs judged to be nonviable bas ...198819290248
behavioral responses of meloidogyne incognita to small temperature changes.small, rapid temperature changes were generated by incandescent radiation, and behavioral responses of meloidogyne incognita juveniles were recorded with high time resolution by computer tracking. temperature changes away from the preferred temperature resulted in decreases in the rate of movement and increases in the rate of change of direction, whether the changes were toward warmer or cooler temperatures. these behavioral changes lasted about 30 seconds. temperature changes toward the preferr ...198819290222
ultrastructural cytochemistry of secretory granules of esophageal glands of meloidogyne incognita.ultrastructural cytochemical tests for several enzymes, proteins, carbohydrates, and nucleic acids were conducted on secretory granules o pound dorsal and subventral esophageal glands of preparasitic second-stage juveniles and the dorsal gland of adult females of meloidogyne incognita. secretory granules in the subventral glands of juveniles stained positive for acid phosphatase. peroxidase, dnase, rnase, cellulase, and nucleic acids were not detected in these granules. secretory granules in the ...198819290195
histopathology of root-knot nematode (meloidogyne incognita) infection on white yam (dioscorea rotundata) tubers.white yam tissues naturally and artificially infected with root-knot nematodes were fixed, sectioned, and examined with a microscope. infective second-stage juveniles of meloidogyne incognita penetrated and moved intercellularly within the tuber. feeding sites were always in the ground tissue layer where the vascular tissues are distributed in the tubers. giant cells were always associated with xylem tissue. they were thin walled with dense cytoplasm and multinucleated. the nuclei of the giant c ...198819290181
reproduction of meloidogyne javanica on corn hybrids and inbreds.the reproductive potential of a meloidogyne javanica population on 64 commercial corn hybrids and 33 corn inbred lines was determined in greenhouse experiments. reproduction was measured by determining rf values (final egg number per initial egg number) and number of eggs per gram of fresh root. all corn hybrids supported reproduction greater than rf = 1.0. rf values for the hybrids ranged from 1.1 for 'pioneer 3147' to 15.5 for 'coker 8575'. three inbreds -- mpt03, nc246, and mp307 -- maintaine ...198819290297
a simple technique for mounting whole root-knot nematode females. 198819290246
dna isolation and gc base composition of four root-knot nematode (meloidogyne spp.) genomes.phenol extraction and cesium trifluoroacetate ultracentrifugation were compared for efficiency in the extraction of dna from eggs and second-stage juveniles of four species of meloidogyne. the second method proved to be more satisfactory in that it yielded larger amounts of dna, shortened the extraction period, and reduced sample handling by eliminating phenol and ether extraction and rnase treatment. it also made possible the extraction of dna: from more than one sample at a time. the mean base ...198819290179
meloidogyne mayaguensis n. sp. (meloidogynidae), a root-knot nematode from puerto rico.meloidogyne mayaguensis n. sp. is described and illustrated from specimens obtained from galled roots of eggplant, solanum melongena l., from puerto rico. the perineal pattern of females is round to ovoid with fine, widely spaced striae. it has occasional breaks of striation laterally and a circular tail tip area lacking striae. the stylet, 15.8 mum long, has reniform knobs that merge gradually with the stylet shaft. males have a high, rectangular, smooth head region, not set off from the body c ...198819290185
interactions between meloidogyne incognita and pratylenchus brachyurus on soybean.interactions among meloidogyne incognita, pratylenchus brachyurus, and soybean genotype on plant growth and nematode reproduction were studied in a greenhouse. coker 317 (susceptible to both nematodes) and gordon (resistant to m. incognita, susceptible to p. brachyurus) were inoculated with increasing initial population densities (pi) of both nematodes individually and combined. m. incognita and p. brachyurus individually usually suppressed shoot growth of both cultivars, but only root growth on ...198819290187
potential for nematode control by mycofloras endemic in the tropics.results of mycological surveys of root-knot and cyst nematodes from tropical regions indicate that most fungal species associated with females or cysts of species of globodera, heterodera, and meloidogyne are those found with nematodes from temperate areas. some fungal species, however, were found in higher frequency in tropical regions than in temperate countries; e.g., cylindrocarpon destructans and ulocladium atrum were the most common species associated with g. pallida and g. rostochiensis c ...198819290202
histology of the interactions of paecilomyces lilacinus with meloidogyne incognita on tomato.excised tomato roots were examined histologically for interactions of the fungus paecilomyces lilacinus and meloidogyne incognita race 1. root galling and giant-cell formation were absent in tomato roots inoculated with nematode eggs infected with p. lilacinus. few to no galls and no giant-cell formation were found in roots dipped in a spore suspension of p. lilacinus and inoculated with m. incognita. numerous large galls and giant cells were present in roots inoculated only with m. incognita. p ...198819290224
effect of meloidogyne incognita and m. javanica on leaf water potential and water use of tobacco.greenhouse lysimeter and field microplot tests were conducted to evaluate the effects of meloidogyne incognita and m. javanica on plant water relations and growth performance of nc 2326 flue-cured tobacco. in the greenhouse, afternoon leaf water potential values at 8-11 weeks after transplanting were lower by as much as 0.22 mpa in plants infected with either nematode than in the control plants. from 11 to 22 weeks, leaf water potential values were similar in all treatments. over the course of t ...198819290249
evaluation of paecilomyces lilacinus as a biocontrol agent of meloidogyne javanica on tobacco.the efficacy of the nematode parasite paecilomyces lilacinus, alone and in combination with phenamiphos and ethoprop, for controlling the root-knot nematode meloidogyne javanica on tobacco and the ability of this fungus to colonize in soil under field conditions were evaluated for 2 years in microplots. combinations and individual treatments of the fungus grown on autoclaved wheat seed, m. javanica eggs (76,000 per plot), and nematicides were applied to specified microplots at the time of transp ...198819290257
reproduction of meloidogyne javanica on plant roots genetically transformed by agrobacterium rhizogenes.reproduction of meloidogyne javanica was compared on several agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. m. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and south australian early dwarf red tomato than on bindweed, tropic tomato, lima bean, or carrot. roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. numbers of females produced in c ...198819290260
nematode population densities and yield of sweet potato and onion as affected by nematicides and time of application.nematode population densities and yield of sweet potato and onion as affected by nematicides and time of application were determined in a 3-year test. population densities of meloidogyne incognita race 1 in untreated plots of sweet potato increased each year, but helicotylenchus dihystera and criconemella ornata did not. ethoprop (6.8 kg a.i./ha) incorporated broadcast in the top 15-cm soil layer each spring before planting sweet potato reduced population densities of nematodes in the soil and i ...198819290295
host suitability of grain sorghum cultivars to meloidogyne spp.grain sorghum cultivars (funk g-499gbr, funk g-611, funk g-522a, funk g-522dr, coker 7723, coker 7675, coker 7623, pioneer b815, pioneer 8222, pioneer 8272) were evaluated in the greenhouse for resistance to populations of meloidogyne incognita race 3, m. arenaria race 2, and m. javanica from south carolina, and m. arenaria race 1 from georgia. all the sorghum cultivars were poor hosts or nonhosts of meloidogyne spp. with fewer than 1 or 2 egg masses per root system in all cultivar x nematode co ...198819290305
resistance in commercial soybean cultivars to six races of heterodera glycines and to meloidogyne incognita.soybean cultivars grown in pots in the greenhouse were tested for resistance by inoculation with meloidogyne incognita or one of six races of heterodera glycines. selected cultivars were tested against each nematode isolate. the numbers of cultivars tested against each h. glycines race and the numbers resistant and (or) moderately resistant were as follows: race 2 - 114 tested, 1 resistant and 9 moderately resistant; race 3 - 170 tested, 56 resistant and 17 moderately resistant; race 4 - 89 test ...198819290307
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