Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| trichomonas vaginalis screening and prevention in order to impact the hiv pandemic: isn't it time we take this infection seriously? | trichomonas vaginalis (tv) is the second most common sexually transmitted infection (sti) in the world. it is associated with significant morbidity in women: pelvic inflammatory disease (pid), concurrent vaginitis and sexually transmitted infections (stis), post-operative infection, and pregnancy complications. tv infection has been implicated in hiv acquisition and transmission in men and women. there are multiple mechanisms to explain this association. tv is not routinely screened for in asymp ... | 2011 | 24470903 |
| detection of trichomonas vaginalis in vaginal swab clinical samples from palestinian women by culture. | background. trichomoniasis is a common sexually transmitted disease caused by trichomonas vaginalis. it is a major health problem worldwide. the world health organization (who) has estimated that 180 million infections are acquired annually worldwide. methodology. vaginal swabs (1207) were cultured for t. vaginalis on trichomonas medium no. 2 (oxoid) soon after specimen collection. the cultures were examined daily using a light microscope to detect the presence of t. vaginalis. results. the prev ... | 2011 | 23724313 |
| improving diagnosis of trichomonas vaginalis infection in resource limited health care settings in sri lanka. | this study was designed to compare diagnosis of trichomoniasis by culture, wet smear examination, and giemsa stain. a modified technique was used to transport and prepare the specimen to ensure parasite viability prior to giemsa staining. | 2011 | 22223992 |
| molecular diagnosis of trichomoniasis in negative samples examined by direct smear and culture. | trichomoniasis is an extremely common sexually transmitted infection (sti) worldwide and is associated with important public health problems, including amplification of hiv transmission. this disease is in forms of symptomatic and asymptomatic in women and may depend on host as well as parasite variables. most of the studies reported from females are based on examination of vaginal secretions and urine samples by direct smear and culture in modified diamond's media. the aim of this study was che ... | 2010 | 22347263 |
| study on its1 gene of iranian trichomonas vaginalis by molecular methods. | trichomoniasis is a worldwide protozoan parasitic disease and metronidazole is a choice drug for its treatment. because of disease importance in public health and its controversial ideas about the prevalence of drug resistance, this study was carried out. | 2010 | 22347260 |
| development of a latex agglutination test as a simple and rapid method for diagnosis of trichomonas vaginalis infection. | trichomoniasis is a worldwide infection and due to its complications rapid and accurate diagnosis of infection especially in pregnant women is very important. in this study, development of a latex agglutination test using native antigens for rapid diagnosis of trichomoniasis is investigated. trichomonas vaginalis was harvested from tyis33 culture medium and anti trichomonas vaginalis antiserum was raised in rabbits. salt precipitation method was used for antibody purification. polyesteren latex ... | 2010 | 23408769 |
| frequency of trichomoniasis in patients admitted to outpatient clinics in hamadan (2007) and relationship between clinical diag-nosis and laboratory findings. | trichomoniasis is recognized as a major sexually transmitted dis-ease (std) in the world and has the highest prevalence and incidence of std. the prevalence strongly is related to cultural and social norms in different socie-ties, in relation to sexual partnership, monogamy, or polygamy. our objective was to describe the frequency and natural history of infection and correlation of clini-cal signs with parasite detection. | 2010 | 22911914 |
| prevalence and etiologic agents of female reproductive tract infection among in-patients and out-patients of a tertiary hospital in benin city, nigeria. | reproductive tract infections are public health problems in women of reproductive age and can result in serious consequences if not treated. | 2010 | 22558550 |
| trichomonas transmembrane cyclases result from massive gene duplication and concomitant development of pseudogenes. | trichomonas vaginalis has an unusually large genome (approximately 160 mb) encoding approximately 60,000 proteins. with the goal of beginning to understand why some trichomonas genes are present in so many copies, we characterized here a family of approximately 123 trichomonas genes that encode transmembrane adenylyl cyclases (tmacs). | 2010 | 20689771 |
| sequence signatures of nucleosome positioning in caenorhabditis elegans. | our recent investigation in the protist trichomonas vaginalis suggested a dna sequence periodicity with a unit length of 120.9 nt, which represents a sequence signature for nucleosome positioning. we now extended our observation in higher eukaryotes and identified a similar periodicity of 175 nt in length in caenorhabditis elegans. in the process of defining the sequence compositional characteristics, we found that the 10.5-nt periodicity, the sequence signature of dna double helix, may not be s ... | 2010 | 20691394 |
| prevention or treatment: the benefits of trichomonas vaginalis vaccine. | trichomoniasis (infection with trichomonas vaginalis) is the most common non-viral sexually transmitted disease (sti) in the world. although treatment is available, most cases occur in developing countries, where accessing healthcare is difficult and facilities are limited. additionally, infection is often asymptomatic and as such goes untreated, creating reservoirs of t. vaginalis that allow the disease to spread within the community. because of this there has been little success in controlling ... | 2010 | 20701891 |
| prevalence and risk factors of sexually transmitted infections and cervical neoplasia in women from a rural area of southern mozambique. | there is limited information on the prevalence of sexually transmitted infections and the prevalence of cervical neoplasia in rural sub-saharan africa. this study describes the prevalence and the etiology of stis and the prevalence of cervical neoplasia among women in southern mozambique. an age-stratified cross-sectional study was performed where 262 women aged 14 to 61 years were recruited at the antenatal clinic (59%), the family-planning clinic (7%), and from the community (34%). at least on ... | 2010 | 20706691 |
| morphologic criteria associated with trichomonas vaginalis in liquid-based cytology. | to evaluate the morphologic criteria associated with trichomonas vaginalis on cytyc (marlborough, massachusetts, u.s.a.) liquid-based cytology and to compare results between 2 types of liquid-based cytology-cytyc and surepath (bd diagnostics-tripath, burlington, vermont, u.s.a). | 2010 | 20715660 |
| in vitro activity of dermaseptin s1 derivatives against genital pathogens. | the aim of this study was to evaluate the biological activity of nine dermaseptin-s1 (drs-s1) derivatives (synthesized by solid-phase methods and purified) against different pathogens causing genital infections (trichomonas vaginalis, herpes simplex virus, papillomavirus). the in vitro activity on t. vaginalis was determined by counting the protozoon in a hemocytometer after vital staining with trypan blue; antiviral activity of the compounds was tested on monolayers of vero cells for herpes sim ... | 2010 | 20718719 |
| trichomonas vaginalis transcription-mediated amplification-based analyte-specific reagent and alternative target testing of primary clinical vaginal saline suspensions. | following wet mount analysis, 255 vaginal saline suspensions were aliquoted to lysis medium for transcription-mediated amplification (tma)-based trichomonas vaginalis analyte-specific reagent testing (asr) (gen-probe, san diego, ca). specimens with visible t. vaginalis were then refrigerated, with additional aliquoting at later intervals. twenty-four wet mount-positive specimens (9.4%) yielded a median luminescent value (x1000, relative light unit [rlu]) of 4736. in contrast, rlu ranged from 1 t ... | 2010 | 20727473 |
| [modified classification of microscopic evaluation of vulvovaginal infections]. | the objective of the study is to examine the role of microscopy using stained smears for diagnosis of vulvovavaginal infections: | 2010 | 20731300 |
| risk of having a sexually transmitted infection in women presenting at a termination of pregnancy clinic in pretoria, south africa. | this study was undertaken to assess the risk of being infected with a known sexually transmitted pathogen at the time of presentation for termination of pregnancy. endocervical and vaginal swabs were collected for the diagnosis of neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis. single infections were found in 21.5% of the women, with c. trachomatis being the commonest (10.0%). mixed infections were found in nine women, with trichomoniasis and chlamydial infections in six. ... | 2010 | 20604651 |
| [clinical laboratory diagnosis of trichomoniasis in patients with internal diseases]. | urogenital trichomoniasis is an infectious inflammatory disease of the urogenital system caused by protozoan trichomonas vaginalis and characterized by rapid dissemination and development of complications. because laboratory diagnosis by microscopic methods encounters difficulty, we undertook detection of t. vaginalis by microscopic study of native and stained (methylene blue, acridine orange, gram, romanovsky-giemsa) uretheral scrapings. 69 patients having no clinical signs of the disease were ... | 2010 | 20608069 |
| medical and legal implications of testing for sexually transmitted infections in children. | testing for sexually transmitted infections (stis) in children presents a number of problems for the practitioner that are not usually faced when testing adults for the same infections. the identification of an sti in a child can have, in addition to medical implications, serious legal implications. the presence of an sti is often used to support the presence or allegations of sexual abuse, and conversely, the identification of an sti in a child will prompt an investigation of possible abuse. th ... | 2010 | 20610820 |
| male circumcision, hiv and sexually transmitted infections: a review. | three randomized controlled trials in sub-saharan africa have shown that circumcision reduces the risk of acquiring hiv infection in men by approximately 60%. in this paper, we review the evidence that male circumcision protects against infection with hiv and other sexually transmitted infections (stis) in men and their female partners. data from the clinical trials indicate that circumcision may be protective against genital ulcer disease, herpes simplex type 2, trichomonas vaginalis and human ... | 2010 | 20622758 |
| [trichomoniasis: prevalence in the female gender in 2004-2005 in sergipe state, brazil]. | trichomoniasis is a disease caused by the protozoan trichomonas vaginalis, which initiates a wide variety of clinical manifestations. it may be associated with human immunodeficiency virus, cervical cancer, infertility and other diseases. its incidence ranges from 20% up to 40% in brazil. the primary channel of transmission is by sexual contact and laboratorial diagnostic may be performed by chain polymerase reaction (cpr), culture medium isolation, fresh vaginal content exam and/or colored by g ... | 2010 | 20640302 |
| susceptibility in vitro of clinically metronidazole-resistant trichomonas vaginalis to nitazoxanide, toyocamycin, and 2-fluoro-2'-deoxyadenosine. | this study investigates the susceptibility of a clinically metronidazole (mz)-resistant isolate of trichomonas vaginalis to alternative anti-trichomonal compounds. the microaerobic minimal inhibitory concentration (mic) of the 5-nitroimidazole (ni) drug, mz, against a typical mz-susceptible isolate of t. vaginalis is around 3.2 microm mz while the clinically, highly mz-resistant isolate has an mic of 50-100 microm. this isolate was cross-resistant to other members of the 5-ni family of compounds ... | 2010 | 20532912 |
| epidemiological synergy of trichomonas vaginalis and hiv in zimbabwean and south african women. | trichomonas vaginalis (t. vaginalis) is the most common nonviral sexually transmitted infection in the world. despite the coexisting global epidemics of t. vaginalis and hiv, little attention has focused on the emerging evidence that t. vaginalis increases susceptibility to, and potentially transmission of, hiv. | 2010 | 20562586 |
| prevalence of sexually transmitted infections and sexual risk behavior among female sex workers in nine provinces in indonesia, 2005. | the objective of this study was to assess the sexually transmitted infection (sti) prevalence and sexual risk behavior among female sex workers (fsws) in indonesia. this cross-sectional 2005 study involved 2500 fsws in nine provinces in indonesia. informed consent was obtained; behavioral and clinical data were collected. specimens were collected for sti testing, endocervical swabs were tested for chlamydia trachomatis and neisseria gonorrhoeae (pcr), vaginal smears were cultured to detect trich ... | 2010 | 20578531 |
| identification and characterization of a surface-associated, subtilisin-like serine protease in trichomonas vaginalis. | summary: trichomonas vaginalis is a protozoan parasite causing trichomonosis, a sexually transmitted infection in humans. this parasite has numerous proteases, most of which are cysteine proteases that appear to be involved in adherence and cytotoxicity of host cells. in this report we identify and characterize a putative subtilisin-like serine protease (sub1). the sub1 gene encodes a 101-kda protein. in silico analyses predict signal and pro-peptides at the n-terminus, and a transmembrane helix ... | 2010 | 20602853 |
| guidelines for the laboratory diagnosis of trichomoniasis in east european countries. | the laboratory diagnosis of sexually transmitted infections in many eastern european countries remains suboptimal. the main objective of the present evidence-based guidelines is to provide comprehensive information regarding the laboratory diagnosis of infections caused by trichomonas vaginalis in east european countries. in particular, the present guidelines recommend: (i) to encourage examination of the wet mounts of vaginal exudates, instead of stained smears, at all clinical settings; (ii) n ... | 2010 | 20236204 |
| antimicrobial and antimalarial activity of cussonia species (araliaceae). | cussonia species are used in african traditional medicine mainly against pain, inflammation, gastro-intestinal problems, malaria and sexually transmitted diseases. | 2010 | 20298772 |
| molecular analysis of clonal trichomonad isolates indicate the existence of heterogenic species present in different birds and within the same host. | trichomonas gallinae is a flagellated protozoon and the etiological agent of avian trichomoniasis. despite its importance, especially in columbiformes and falconiformes, only a few molecular studies have yet been performed in order to investigate the degree of genetic diversity and cross-transmissibility between different isolates of this parasite. to address these questions 63 clonal cultures of trichomonas spp. isolates were established by successful isolation of single trichomonads from a mix ... | 2010 | 20471174 |
| the tropical brown alga lobophora variegata: a source of antiprotozoal compounds. | lobophora variegata, a brown alga collected from the coast of the yucatan peninsula, mexico, was studied for antiprotozoal activity against giardia intestinalis, entamoeba histolytica and trichomonas vaginalis. the whole extract showed the highest activity against t. vaginalis, with an ic(50) value of 3.2 microg/ml. for the fractions, the best antiprotozoal activity was found in non-polar fractions. the chloroform fraction of the extract contained a major sulfoquinovosyldiacylglycerol (sqdg), id ... | 2010 | 20479979 |
| methyl jasmonate induces cell death and loss of hydrogenosomal membrane potential in trichomonas vaginalis. | trichomonas vaginalis is an important human parasite of the urogenital tract. jasmonates are a group of small lipids that are produced in plants and function as stress hormones. naturally occurring methyl jasmonate (mj) has been used to treat several types of cancer cells and it is cytotoxic to protistan parasites. it has been suggested that mitochondria are the target organelles of jasmonates. here, we tested this drug against t. vaginalis. although metronidazole has been the drug of choice for ... | 2010 | 20483382 |
| the burden and risk factors of sexually transmitted infections and reproductive tract infections among pregnant women in zimbabwe. | sexually transmitted infections (stis) and reproductive tract infections (rtis) are responsible for high morbidity among women. we aim to quantify the magnitude of the burden and risk factors of sti/rti s among pregnant women in zimbabwe. | 2010 | 20492681 |
| screening for trichomonas vaginalis in high-risk adolescent females with a new transcription-mediated nucleic acid amplification test (naat): associations with ethnicity, symptoms, and prior and current stis. | the importance of diagnosing trichomoniasis is highlighted by its strong association with hiv acquisition and viral shedding. the low sensitivity of wet preparation and often asymptomatic nature of trichomoniasis results in failure to recognize and treat this sexually transmitted infection. the purpose of this study was to evaluate the feasibility of screening high-risk adolescent females using a new highly sensitive and specific naat assay. | 2010 | 20493735 |
| multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens. | the objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mpcr) based reverse line blot (rlb) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, ureaplasma parvum, mycoplasma genitalium, mycoplasma hominis and trichomonas vaginalis). species-specific biotin-labelled primer pairs were used in a single mpcr ... | 2010 | 20498099 |
| patient-delivered partner treatment and trichomonas vaginalis repeat infection among human immunodeficiency virus-infected women. | repeat infections with trichomonas vaginalis (tv) among human immunodeficiency virus (hiv)-infected women are common and may increase the risk of hiv transmission. patient delivered partner treatment (pdpt) has been shown to reduce repeat infections of other sexually transmitted diseases. the purpose of this study was to evaluate adherence to pdpt and possible causes of repeat tv infection among hiv-infected women. | 2010 | 20502393 |
| etiology and sti/hiv coinfections among patients with urethral and vaginal discharge syndromes in south africa. | this study was undertaken to establish the etiology of the male urethral discharge (muds) and vaginal discharge (vds) syndromes, to determine the prevalence of other sexually transmitted infections (sti) and human immunodeficiency virus (hiv) coinfections, and to examine associations between stis and hiv serostatus among sti patients in south africa. | 2010 | 20502394 |
| efficacy of punica granatum extract on in-vitro and in-vivo control of trichomonas vaginalis. | trichomoniasis vaginalis is now an important worldwide health problem. metronidazole has so far been used in treatment, but the metronidazole-resistant strains and unpleasant adverse effects have been developed. treatment of patients with metronidazole refractory vaginal trichomoniasis constitutes a major therapeutic challenge and treatment options are extremely limited. the last 7 years have seen over seven times as many publication indexed by midline dealing with pomegranate (punica granatum) ... | 2010 | 20503601 |
| observation of probable persistent, undetected trichomonas vaginalis infection among hiv-positive women. | 2010 | 20518674 | |
| male circumcision for the prevention of acquisition and transmission of sexually transmitted infections: the case for neonatal circumcision. | the american academy of pediatrics (aap) male circumcision policy states that while there are potential medical benefits of newborn male circumcision, the data are insufficient to recommend routine neonatal circumcision. since 2005, however, 3 randomized trials have evaluated male circumcision for prevention of sexually transmitted infections. the trials found that circumcision decreases human immunodeficiency virus acquisition by 53% to 60%, herpes simplex virus type 2 acquisition by 28% to 34% ... | 2010 | 20048246 |
| correlates of cervical mycoplasma genitalium and risk of preterm birth among peruvian women. | mycoplasma genitalium is associated with cervicitis and pelvic inflammatory disease in nonpregnant women. we investigated associations between cervical m genitalium, demographic and behavioral risk factors for sexually transmitted infection and preterm birth among low-income peruvian women. | 2010 | 20051932 |
| identification of two novel trichomonas vaginalis eif-5a genes. | the eukaryotic translation initiation factor 5a (eif-5a) is highly conserved and is the only protein that is known to contain the unique and essential amino acid residue hypusine. synthesis of hypusine is essential for the function of eif5a in eukaryotic cell proliferation and survival. in this study, we identified two novel eukaryotic translation initiation factor 5a (eif-5a) genes in trichomonas vaginalis. the tveif-5a1 and tveif-5a2 putative genes were localized in different contigs, both con ... | 2010 | 20060503 |
| the prevalence, incidence and risk factors of herpes simplex virus type 2 infection among pregnant zimbabwean women followed up nine months after childbirth. | herpes simplex virus type 2 (hsv-2) is the leading cause of genital ulcer disease worldwide. the virus can be transmitted to neonates and there are scarce data regarding incidence of hsv-2 among women in pregnancy and after childbirth. the aim of this study is to measure the incidence and risk factors for hsv-2 infection in women followed for 9 months after childbirth. | 2010 | 20064273 |
| acetate formation in the energy metabolism of parasitic helminths and protists. | formation and excretion of acetate as a metabolic end product of energy metabolism occurs in many protist and helminth parasites, such as the parasitic helminths fasciola hepatica, haemonchus contortus and ascaris suum, and the protist parasites, giardia lamblia, entamoeba histolytica, trichomonas vaginalis as well as trypanosoma and leishmania spp. in all of these parasites acetate is a main end product of their energy metabolism, whereas acetate formation does not occur in their mammalian host ... | 2010 | 20085767 |
| the flavin inhibitor diphenyleneiodonium renders trichomonas vaginalis resistant to metronidazole, inhibits thioredoxin reductase and flavin reductase, and shuts off hydrogenosomal enzymatic pathways. | infections with the microaerophilic protozoan parasite trichomonas vaginalis are commonly treated with metronidazole, a 5-nitroimidazole drug. metronidazole is selectively toxic to microaerophiles and anaerobes because reduction at the drug's nitro group, which is a precondition for toxicity, occurs only quantitatively in these organisms. in our previous work we identified the flavin enzyme thioredoxin reductase as an electron donor to 5-nitroimidazole drugs in t. vaginalis and observed that hig ... | 2010 | 20093143 |
| trichomonas vaginalis vast bspa-like gene family: evidence for functional diversity from structural organisation and transcriptomics. | trichomonas vaginalis is the most common non-viral human sexually transmitted pathogen and importantly, contributes to facilitating the spread of hiv. yet very little is known about its surface and secreted proteins mediating interactions with, and permitting the invasion and colonisation of, the host mucosa. initial annotations of t. vaginalis genome identified a plethora of candidate extracellular proteins. | 2010 | 20144183 |
| a cross-sectional study of reported symptoms for sexually transmissible infections among female sex workers in papua new guinea. | sexually transmissible infections (stis) are common in female sex workers (fsws), most of which are asymptomatic and therefore under-reported. our aim was to determine the sensitivity and specificity of reported symptoms obtained via questionnaire augmented with leukocyte esterase (le) urine dipstick test for the detection of chlamydia trachomatis (ct), neisseria gonorrhea (ng) and trichomonas vaginalis (tv) detected using polymerase chain reaction (pcr). | 2010 | 20152100 |
| trichomonas vaginalis: dehydroepiandrosterone sulfate and 17beta-estradiol alter ntpdase activity and gene expression. | we investigated the effect of dehydroepiandrosterone sulfate (dheas) and 17beta-estradiol on ntpdase activity in fresh clinical (vp60) and long-term-grown (30236 atcc) isolates of trichomonas vaginalis followed by ntpdase gene transcriptional analysis. atp hydrolysis was activated in vitro by 17beta-estradiol (0.01-1.0microm) in the vp60 isolate. treatment for 2h with 17beta-estradiol (0.01-1microm) promoted an inhibition in nucleotide hydrolysis in the 30236 isolate whereas the 12h-treatment pr ... | 2010 | 20159012 |
| point-of-care testing for sexually transmitted infections increases awareness and short-term abstinence in adolescent women. | to evaluate the effect of point-of-care (poc) testing for sexually transmitted infections (stis) on reported awareness of test results and sti risk-reduction behaviors in adolescents. | 2010 | 20159505 |
| intravaginal practices, vaginal infections and hiv acquisition: systematic review and meta-analysis. | intravaginal practices are commonly used by women to manage their vaginal health and sexual life. these practices could, however, affect intravaginal mucosal integrity. the objectives of this study were to examine evidence for associations between: intravaginal practices and acquisition of hiv infection; intravaginal practices and vaginal infections; and vaginal infections and hiv acquisition. | 2010 | 20161749 |
| trichomonas vaginalis nucleoside triphosphate diphosphohydrolase and ecto-5'-nucleotidase activities are inhibited by lycorine and candimine. | drug discovery from plants plays an important role in the pharmaceutical therapy field and the alkaloids lycorine and candimine are candidates for this purpose. trichomonas vaginalis is a parasite that infects the human urogenital tract and causes trichomonosis, the most prevalent non-viral sexually transmitted disease. ecto-nucleotidases including nucleoside triphosphate diphosphohydrolase (ntpdase) members, which hydrolyses extracellular atp (adenosine triphosphate) and adp (adenosine diphosph ... | 2010 | 20176129 |
| determinants of hiv type 1 shedding from genital ulcers among men in south africa. | our study evaluated correlates of human immunodeficiency virus (hiv)-1 lesional shedding among men with genital ulcer disease (gud). | 2010 | 20178417 |
| prevalence of trichomonas vaginalis infection in women attending a major gynaecological hospital in greece: a cross-sectional study. | the prevalence of trichomonas vaginalis is not accurately estimated, since it is not a reportable disease. | 2010 | 20203225 |
| regulation of gene expression in protozoa parasites. | infections with protozoa parasites are associated with high burdens of morbidity and mortality across the developing world. despite extensive efforts to control the transmission of these parasites, the spread of populations resistant to drugs and the lack of effective vaccines against them contribute to their persistence as major public health problems. parasites should perform a strict control on the expression of genes involved in their pathogenicity, differentiation, immune evasion, or drug r ... | 2010 | 20204171 |
| paromomycin treatment of recalcitrant trichomonas vaginalis. | we report a 49-year-old woman with a five-year history of persistent trichomonas vaginalis infection. treatment with several courses of metronidazole and tinidazole failed to resolve her symptoms. a single course of intravaginal paromomycin was effective in clearing the infection. | 2010 | 20215633 |
| delayed human neutrophil apoptosis by trichomonas vaginalis lysate. | neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. moreover, it is thought that trichomonal lysates and excretory-secretory products (esp), as well as live t. vaginalis, could possibly interact with neutrophils in local tissues, including areas of inflammation induced by t. vaginalis in humans. the a ... | 2010 | 20333279 |
| prevalence and comparison of diagnostic methods for trichomonas vaginalis infection in pregnant women in argentina. | the objectives of this study were to conduct a prevalence survey of trichomoniasis in pregnant women and to evaluate the utility of different methods for its diagnosis. a total of 597 vaginal exudates from pregnant women who were examined at the hospital de clinicas in buenos aires, argentina from 1 august 2005 to 31 january 2007, were prospectively and consecutively evaluated. the investigation of trichomonas vaginalis was made by different microscopic examinations, and culture on liquid medium ... | 2010 | 20333287 |
| urinary balantidiasis: diagnosis at a glance by urine sediment examination. | a 56-year-old caucasian man with non-hodgkin's lymphoma, who had previously been treated with prolonged intensive chemotherapy, was hospitalized for an acute and reversible kidney injury of multifactorial origin. the urinary sediment examination, performed daily, demonstrated the presence of renal tubular cells and renal tubular cell casts. surprisingly, it also showed the presence of trophozoites of the protozoan balantidium coli, which were identified on the basis of its characteristic morphol ... | 2010 | 20349417 |
| correlation of trichomonas vaginalis to bacterial vaginosis: a laboratory-based study. | this study aimed to define the occurrence of different organisms causing vulvovaginitis; to evaluate different laboratory methods used for diagnosis of trichomonas vaginalis (t. vaginalis); and to evaluate the direct score system and clue cell method compared with culture for diagnosis of bacterial and t. vaginalis vaginosis. | 2010 | 20351456 |
| trichomonas vaginalis prevalence, incidence, risk factors and antibiotic-resistance in an adolescent population. | to determine the prevalence and incidence of trichomoniasis, risk factors for infection, and the prevalence of metronidazole and tinidazole-resistant trichomonas vaginalis (t. vaginalis) in female adolescents. | 2010 | 20351623 |
| early smoking initiation, sexual behavior and reproductive health - a large population-based study of nordic women. | to investigate associations between early smoking initiation, risk-taking behavior and reproductive health. | 2010 | 20353801 |
| evaluation of cervical screening results in a population at normal risk. | to evaluate the frequency of dysplasia and other cervical conditions in a population at normal risk, and compare demographic characteristics of the women with normal and abnormal results. | 2010 | 20362986 |
| a pilot study on the prevalence of sexually transmitted infections among clients of brothel-based female sex workers in jessore, bangladesh. | the prevalence of bacterial sexually transmitted infections among clients of female sex workers (fsws) was studied. a total of 156 condoms were collected from clients of 13 sex workers during three consecutive days. condoms used by clients of fsws were collected and dna was extracted from the seminal fluid and used for the diagnosis of neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis by polymerase chain reaction. the prevalence of n. gonorrhoeae, c. trachomatis and t. vagin ... | 2010 | 20378907 |
| rapid detection of coinfections by trichomonas vaginalis, mycoplasma hominis, and ureaplasma urealyticum by a new multiplex polymerase chain reaction. | we developed a multiplex polymerase chain reaction (m-pcr) assay to simultaneously detect trichomonas vaginalis, mycoplasma hominis, and ureaplasma urealyticum. the test is extremely specific and has a sensitivity of 10 cells for t. vaginalis and u. urealyticum and of 1 cell for m. hominis. the technique was validated on vaginal swabs from 240 women presenting symptoms of vaginitis, and results were compared with data obtained using microscopic and culture techniques on the same patients. the m- ... | 2010 | 20385350 |
| [local combined therapy of vaginal infections by nifuratel-nistatin]. | a test included 40 women in the reproductive age with clinical symptoms of vaginitis and microbiological examination. they were treated by combined therapy of vaginal tablets of nifuratel, 500 mg and nistatin 200 000 i. u. during six days, after which they underwent gynaecological reexamination and repeated microbiological examination of vaginal and cervical smears. an analiysis of vaginal secretion found bacterial flora in 34 smears (65%), fungus (candida albicans) in 15 (24%) and trichomonas v ... | 2010 | 20387731 |
| gardnerella, trichomonas vaginalis, candida, chlamydia trachomatis, mycoplasma hominis and ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women. | this study aimed to establish the different prevalence of the microorganisms investigated in the two groups considered: fertile women with symptoms and asymptomatic women with infertility problems. the data from women (n= 952) investigated for two years for quality of genital discharge and the presence of gardnerella vaginalis, trichomonas vaginalis, candida species, streptococcus agalactiae, mycoplasma hominis, ureaplasma urealyiticum and chlamydia trachomatis were retrospectively analyzed. in ... | 2010 | 20402416 |
| treatment of trichomoniasis in pregnancy in sub-saharan africa does not appear to be associated with low birth weight or preterm birth. | to determine whether treatment of trichomoniasis increases the risk of prematurity. | 2010 | 20429491 |
| synthesis and biological activity of 2-(trifluoromethyl)-1h-benzimidazole derivatives against some protozoa and trichinella spiralis. | a series of 2-(trifluoromethyl)-1h-benzimidazole derivatives (1a-1i) were synthesized via phillips cyclocondensation of a substituted 1,2-phenylenediamine and trifluoroacetic acid. the synthesized compounds were evaluated in vitro against various protozoan parasites: giardia intestinalis, entamoeba histolytica, trichomonas vaginalis and leishmania mexicana, and they showed nanomolar activities against the first three protozoa tested. the compounds were also tested in vitro and in vivo against th ... | 2010 | 20430484 |
| trichomonas vaginalis: investigation of a novel diagnostic method in urine samples using cysteine proteinase 4 gene and pcr technique. | trichomonas vaginalis is the agent of a highly prevalent sexually transmitted disease that leads to vaginitis, urethritis, ectocervicitis and has been associated with human immunodeficiency virus (hiv). detection of t. vaginalis based on wet-mount microscopy and culture methods is insensitive and time consuming, respectively. thus the quest for reliable pcr techniques of t. vaginalis in vaginal discharge and urine sample is more importance. in this study, 500 urine and vaginal-discharge samples ... | 2010 | 20434441 |
| should expedited partner treatment for women with trichomonas vaginalis be recommended? | 2010 | 20453719 | |
| a randomized controlled trial of partner notification methods for prevention of trichomoniasis in women. | trichomoniasis is associated with adverse pregnancy outcomes and increased risk for human immunodeficiency virus. males are usually asymptomatic, and thus there is heavy reliance on partner notification for identifying infected male partners. the usual approach is partner referral but it is estimated that only a minority of men seek care. we conducted a randomized trial to compare the effectiveness of 3 methods of partner notification. | 2010 | 20453720 |
| [frequency of curable sexually transmitted infections among registered female sex-workers in ankara city]. | sex-workers are considered as the high-risk population for sexually transmitted infections (stis). early diagnosis and treatment of curable stis in this high-risk group have crucial importance in sti control and prevention of complications and transmission of infection. in this study, 146 registered female sex-workers in ankara city were screened with rapid diagnostic tests (rdt) for causative agents of curable stis such as, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis and ... | 2010 | 20455407 |
| proteome analysis of the surface of trichomonas vaginalis reveals novel proteins and strain-dependent differential expression. | the identification of surface proteins on the plasma membrane of pathogens is of fundamental importance in understanding host-pathogen interactions. surface proteins of the extracellular parasite trichomonas are implicated in the initial adherence to mucosal tissue and are likely to play a critical role in the long term survival of this pathogen in the urogenital tract. in this study, we used cell surface biotinylation and multidimensional protein identification technology to identify the surfac ... | 2010 | 20467041 |
| trichomonas vaginalis kills and eats--evidence for phagocytic activity as a cytopathic effect. | this study reports that the cytopathic effect of trichomonas vaginalis, an important human parasite of the urogenital tract, occurs due to mechanical stress and subsequent phagocytosis of the necrotic cells. the investigation was done using a primary culture of bovine oviduct epithelial cells (boecs), grown either in monolayers or as floating cells. trophozoites displaying different virulence levels were co-incubated with boecs for times varying between 1 min and 48 h. analyses were performed us ... | 2010 | 19723359 |
| genomic organization and promoter analysis of the trichomonas vaginalis core histone gene families. | core histone gene is a well-established model to study eukaryote gene transcription regulation mechanism. however, the protozoan core histone gene regulation mechanism remains largely unknown. in this study, we observed almost all protozoan trichomonas vaginalis core histone genes (60/74) organize as gene pairs in a head-to-head manner, thus facilitating the divergent transcription of both partners. additionally, the majority of both t. vaginalis core histone genes pairs (50/60) and solitary gen ... | 2010 | 19744576 |
| inflammatory events as detected in cervical smears and squamous intraepithelial lesions. | the dutch cytological coding system, kopac, enables to code for eight inflammatory events, that is koilocytosis (related to human papillomavirus (hpv)), trichomonas, dysbacteriosis [related to bacterial vaginosis (bv)], candida, gardnerella, actinomyces, chlamydia, and non-specific inflammation (leucocytosis). this study presents an analysis of 1,008,879 smears. of each smear, the age of the woman and the reason for smear taking (screening or indication) was available. the cytoscores (per mille) ... | 2010 | 19795485 |
| [diagnostic effectiveness of wet mount examination versus nucleic acid hybridisation for the diagnosis of trichomoniasis]. | 2010 | 19892437 | |
| histomonas meleagridis possesses three alpha-actinins immunogenic to its hosts. | histomonas meleagridis is a protozoan parasite known to cause histomonosis (syn. typhlohepatitis) in poultry. due to the economic losses which the disease entails, there is an urgency to understanding its pathogenesis. in the present investigation, three partial cdna sequences encoding heterogenic alpha-actinins previously identified for h. meleagridis were completed and characterized. these three h. meleagridis alpha-actinin isoforms were named alpha-actinin1, alpha-actinin2, and alpha-actinin3 ... | 2010 | 19896981 |
| etiology and determinants of sexually transmitted infections in karnataka state, south india. | syndromic case management remains the cornerstone for sti (sexually transmitted infection) treatment in many countries. we undertook this study to better understand the etiology of stis in adults in south india and to inform sti management guidelines. | 2010 | 19901864 |
| imidazole derivatives as possible microbicides with dual protection. | twenty seven derivatives (2-28) of 2-(2-methyl-5-nitro-1h-imidazol-1-yl)ethanol were synthesized and evaluated for anti-trichomonas, spermicidal and antifungal activities. twenty six compounds were active against trichomonas vaginalis at mic ranging from 1-42 microm and seven compounds (9,18,19,22,24,26,28) immobilized 100% human spermatozoa at 1% concentration (w/v). twenty three compounds (2,3,5,8-26,28) exhibited antifungal activity at 25-50 microg/ml concentration. seven compounds (9,18,19,2 ... | 2010 | 19906466 |
| axenization and optimization of in vitro growth of clonal cultures of tetratrichomonas gallinarum and trichomonas gallinae. | a rapid and simple procedure was established to obtain clonal axenic cultures of tetratrichomonas gallinarum and trichomonas gallinae and to optimize their in vitro growth conditions. medium 199 was used for axenization of two genetically different clones of t. gallinarum and t. gallinae. six different media were used to optimize the growth behaviour of axenically grown parasites: medium 199, tym, tyi-s-33, hollander fluid (hf), trichomonas vaginalis (tv) and modified tv media. the highest cell ... | 2010 | 19766633 |
| high prevalence of bacterial vaginosis in adolescent girls in a tropical area of ecuador. | bacterial vaginosis (bv) is a common clinical syndrome, but data are scarce on the bv prevalence in tropical regions among sexually active and virgin adolescents. to estimate the prevalence of bv among adolescent girls in an ecuadorian coastal town, girls were asked to complete a questionnaire on risk factors for bv and vaginal samples were examined. bacterial vaginosis was present in 31.5% of 213 girls, and the prevalence was similar in self-reported virgin and sexually active girls (or 1.06, 9 ... | 2010 | 19874295 |
| trichomonas vaginalis: underdiagnosis in urban australia could facilitate re-emergence. | trichomonas vaginalis (tv) has a low profile in urban sexually transmitted infection (sti) clinics in many developed countries. the objective of this study was to determine the true prevalence of tv in an australian urban sexual health setting using sensitive molecular diagnostic techniques. | 2010 | 19880969 |
| high prevalence of high-risk human papillomavirus infection among women with trichomonas vaginalis infection on monolayer cytology. | to evaluate the risk of high-risk human papillomavirus (hpv) infection in women with trichomonas vaginalis infection, and the reason remains unclear. | 2010 | 19940998 |
| immunoproteomics of the active degradome to identify biomarkers for trichomonas vaginalis. | trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (cps); some are involved in trichomonal pathogenesis, express during infection, and antibodies against cps have been detected in patient sera. the goal of this study was to identify the antigenic proteinases of t. vaginalis as potential biomarkers for trichomonosis. the proteases detected when t. vaginalis protein extracts are incubated without protease inhibitors, the trichomonad-active degradome, and the immu ... | 2010 | 19957290 |
| diagnosis of trichomonas vaginalis in female children and adolescents. | 2010 | 19963412 | |
| cloning and sequencing of adhesion protein gene of trichomonas gallinae from pigeon. | the adhesion protein (ap) gene of trichomonas gallinae from pigeon was cloned and sequenced. the first-strand cdna of the ap gene of t. gallinae from pigeon was amplified by reverse transcription polymerase chain reaction (rt-pcr) with total rna extracting kit and cloned in the vector pmd18-t. the recombinant plasmid was identified by pcr and restriction endonuclease, and the positive clone was sequenced and analysed by comparing the sequence similarity with other sequences in the genbank. the a ... | 2010 | 19939572 |
| diagnostic utility of the genital gram stain in ed patients. | the study aimed to determine the diagnostic usefulness of the genital gram stain in an emergency department (ed) population. | 2010 | 20006195 |
| hydrogenosomes of laboratory-induced metronidazole-resistant trichomonas vaginalis lines are downsized while those from clinically metronidazole-resistant isolates are not. | trichomonas vaginalis is the most common sexually transmitted protozoan in the world and its resistance to metronidazole is increasing. the purpose of this study was to demonstrate that clinical metronidazole resistance in t. vaginalis does not occur via the same mechanism as laboratory-induced metronidazole resistance--that is, via hydrogenosome down sizing. ultrathin sections of this parasite were examined using transmission electron microscopy and the size and area of the cell and hydrogenoso ... | 2010 | 20015182 |
| prevalence and correlates of trichomonas vaginalis among incarcerated persons assessed using a highly sensitive molecular assay. | we describe the epidemiology of trichomonas vaginalis (tv) among san francisco county jail inmates using aptima tv analyte-specific reagents on remnant urine. we detected tv in 15/713 (2.1%) men and 95/297 (32.0%) women. among women, increased age was significantly associated with tv. the benefits of tv screening should be determined. | 2010 | 20023598 |
| immunohistochemical study of the vaginal inflammatory response in experimental trichomoniasis. | in the present paper, the acute and subchronical inflammatory processes of the vaginal epithelial were studied in mice experimentally infected with two trichomonas vaginalis strains of different pathogenicity, by means of histological and immunological methods. there was an increase in the stratified epithelium layers as well as edema produced by the increase of vascularization in the propia submucosa and infiltration of leukocytes. the proliferation of the vaginal epithelium favors the settleme ... | 2010 | 20025844 |
| [procedure for trichomonas vaginalis cultivation]. | the investigation was undertaken to design a nutrient medium that could reduce the duration of a culture test in the diagnosis of trichomoniasis and to comparatively evaluate its effectiveness. a procedure was proposed to cultivate trichomonas vaginalis, which was based on the use of the nutrient medium containing casein hydrolysate, yeast autolysate, and serum, added by 0.6 ml of liquid ferrum lec (iron (iii) hydroxide polyisomaltose), which reduced the time taken to identify trichomonas vagina ... | 2010 | 21400718 |
| relationship between infectious agents for vulvovaginitis and skin color. | many factors influence occurrences of vulvovaginitis. the aims here were to assess skin color and age-related differences in the vaginal flora and occurrences of vulvovaginitis. | 2010 | 21308158 |
| cryptic diversity of free-living parabasalids, pseudotrichomonas keilini and lacusteria cypriaca n. g., n. sp., as inferred from small subunit rdna sequences. | ultrastructural and molecular phylogenetic evidence indicate that the parabasalia consists of seven main subgroups: the trichomonadida, honigbergiellida, hypotrichomonadida, tritrichomonadida, cristamonadida, spirotrichonymphida, and trichonymphida. only five species of free-living parabasalids are known: monotrichomonas carabina, ditrichomonas honigbergii, honigbergiella sp., tetratrichomonas undula, and pseudotrichomonas keilini. phylogenetic analyses show that free-living species do not form ... | 2010 | 20880033 |
| towards a systematic characterization of the antiprotozoal activity landscape of benzimidazole derivatives. | parasitic infections caused by the protozoa trichomonas vaginalis and giardia intestinalis still represent a major problem in developing countries. despite the fact that benzimidazoles are promising compounds with activity against both protozoa, systematic studies to characterize and compare their structure-activity relationships (sar) are limited. herein, we report a systematic characterization of the sar of 32 benzimidazoles with activity against t. vaginalis and g. intestinalis. the analysis ... | 2010 | 20888242 |
| sphingolipids in parasitic protozoa. | the surface of most protozoan parasites relies heavily upon lipid-anchored molecules, to form protective barriers and play critical functions required for infectivity. sphingolipids (sls) play important roles through their abundance and involvement in membrane microdomain formation, as well as serving as the lipid anchor for many of these molecules and in some but possibly not all species, as important signaling molecules. interactions of parasite sphingolipid metabolism with that of the host ma ... | 2010 | 20919659 |
| estimating the number of unlicensed brothels operating in melbourne. | to estimate the number of unlicensed brothels operating in melbourne, australia, and the sexual health of the women working in them. | 2010 | 20920108 |
| cervicovaginal shedding of hiv type 1 is related to genital tract inflammation independent of changes in vaginal microbiota. | we examined the relationship of proinflammatory vaginal cytokines and secretory leukocyte protease inhibitor (slpi) with genital hiv-1 shedding after controlling for genital coinfections. fifty-seven hiv-1-infected women in seattle, wa (n = 38) and rochester, ny (n = 19) were followed every 3-4 months for a total of 391 visits. at each visit, plasma and cervicovaginal lavage (cvl) were tested for hiv-1 rna using qpcr. vaginal samples were tested for bacterial vaginosis, yeast, hydrogen peroxide- ... | 2010 | 20929397 |
| trichomonas vaginalis 62 kda proteinase as a possible virulence factor. | the aim of this study was to analyze the presence of 62 kda proteinase and anti-62 kda proteinase antibody in clinical samples of symptomatic and asymptomatic infected women. proteinase was detected in all the swabs vaginal of infected women. significantly, amounts of antigen (mean optical density (od) values) were detected in swabs vaginal of symptomatic as compared to asymptomatic women. this protein was not detected in the group of patients with trichomonas vaginalis-culture-negative results ... | 2010 | 20938688 |
| [hydrogen peroxide treatment for vaginal trichomoniasis. 1955]. | 2010 | 20939246 | |
| clinicopathological study of papanicolaou (pap) smears for diagnosing of cervical infections. | cervical infections are not uncommon in our population especially in young and sexually active women. one thousand samples of married women, aged between 20 and 70 years, were studied by conventional papanicolaou smears. these samples were examined in the department of pathology, king edward medical university, lahore from january 2007 to june 2009. only cases without (pre)neoplastic cytology were included. six types of infections were diagnosed cytologically. the overall frequency of normal, in ... | 2010 | 20949462 |
| neonatal trichomonas vaginalis infection: a case report and review of literature. | neonatal infection with trichomonas vaginalis is an unusual occurrence. we present a case of t. vaginalis found on routine urinalysis in a five-day-old neonate born at 29 weeks gestational age. the patient was treated with metronidazole and had complete resolution of the infection. this report discusses the significance of diagnosis and treatment of t. vaginalis in the neonate. | 2010 | 20975098 |