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examination of ribosome-like particles in isolated prolamellar bodies.potential methods for the preparation of fractions enriched in prolamellar bodies (plbs) were examined in detail. sucrose density gradient centrifugation methods gave fractions consisting almost exclusively of plbs whilst those methods employing differential centrifugation were quite successful but contained greater quantities of lamellar membranes. greater difficulty was experienced in obtaining detached plbs which retained their "ribosome-like" lattice particles. no modification to density gra ...197724419578
metabolism of indole-3-acetic acid: iv. biological properties of amino acid conjugates.the biological activity of 20 l-alpha-amino acid conjugates of indole-3-acetic acid (iaa) to stimulate cell elongation of avena sativa coleoptile sections and to stimulate growth of soybean cotyledon tissue cultures has been examined at concentrations of 10(-4) to 10(-7)m. in the avena coleoptile test, most of the amino acid conjugates stimulated elongation. several of the conjugates stimulated as much elongation as iaa but their half-maximum concentrations tended to be higher. some of the more ...197716659795
formation of n-acetylglutamate by extracts of higher plants.the enzymic synthesis of n-acetylglutamate was studied in extracts of higher plant tissues, especially in sugar beet leaves (beta vulgaris l.). sugar beet leaves had an enzyme that transferred the acetyl group either from acetyl-coa or from n(2)-acetylornithine to glutamate. the enzyme was so unstable that special precautions were necessary for its detection and appreciable purification was impossible. the km values were 2.5 and 0.025 mm for acetyl-coa and n(2)-acetylornithine, respectively. the ...197716659918
long distance translocation of sucrose, serine, leucine, lysine, and carbon dioxide assimilates: ii. oats.to establish whether several amino acids were equally able to enter the phloem of oat (avena sativa l.) plants and be transported, several (14)c-labeled amino acids were applied individually to an abraded spot on a fully expanded source leaf. the base of an immature sink leaf was monitored with a gm tube for time and rate of arrival of radioactivity. transport of (14)c-sucrose and (14)co(2) assimilates was measured for a comparison. the applied l-serine, l-lysine, and l-leucine, as well as sucro ...197716659821
use of i-labeled phytochrome to quantitate phytochrome binding to membranes of avena sativa.purified oat phytochrome was labeled with (125)i without altering the photoreversibility or absorbance properties of the pigment. the radiolabeled phytochrome was used in experiments in vitro to quantitate the binding of the pigment to both crude and purified membrane preparations from oat tissue. after the membranes were allowed to react with (125)i-labeled phytochrome, washed free of unbound material, and pelleted, they were found to have significant levels of radioactivity bound to them. qual ...197716592450
attempts to detect cyclic adenosine 3':5'-monophosphate in higher plants by three assay methods.endogenous levels of cyclic adenosine-3':5'-monophosphate in coleoptile first leaf segments of oat (avena sativa l.), potato (solanum tuberosum l.) tubers, tobacco (nicotiana tabacum l.) callus, and germinating seeds of lettuce (lactuca sativa l.) were measured with a modified gilman binding assay and a protein kinase activation assay. the incorporation of adenosine-8-(14)c into compounds with properties similar to those of cyclic amp was also measured in studies with germinating lettuce seeds. ...197616659419
extraction and purification of a substance with luteinizing hormone releasing activity from the leaves of avena sativa.attempts were made to purify the lh-releasing substance extracted from the leaves of avena sativa by means of two-step chromatographic procedures using a weakly acidic ion-exchange resin (cg-50,type ii) and deae-sephadex a-25 (coarse) with successful results. for preliminary fractionation of such starting materials as dried leaves, fresh leaves, and acetone-extracted powder(crude extracts), 5% acetate-buffered active carbon proved to be more effective than starch zone electrophoresis. from its b ...1976781913
red light-enhanced phytochrome pelletability: re-examination and further characterization.red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: avena sativa l., secale cereale l., zea mays l., cucurbita pepo l., sinapis alba l., pisum sativum l., helianthus anuus l., raphanus sativus l., glycine max (l.) merr., phaseolus vulgaris l., and lupinus albus l. this enhanced pelletability was observed in all 11 plants following in situ irradiation (in vivo binding) but only in sinapis and cucurbita after irradiation of crude extracts (in vitro bin ...197616659745
pharmacotherapy with avena sativa - a double blind study.hundred non-hospitalized smokers with an average consumption of 20 cigarettes per day were treated with an alcoholic extract of avena sativa for disaccustoming. the study was double blind. by using query-sheets personal contact was excluded. the first result was a placebo-effect of 35% for disaccustoming of smokers and no statistically significant effect of the extract of avena sativa. the second result was a difference of disaccustoming between light and heavy smokers. it was shown, that the ra ...1976794001
characterization of passive ion transport in plasma membrane vesicles of oat roots.the passive influx and efflux of inorganic ions across plasma membrane vesicles purified from extracts of avena sativa roots were investigated. uptake was measured by incubating the vesicles in a radioisotope for various times. the "loaded" vesicles were separated from the external solution by gel filtration. efflux was measured by dialyzing the preloaded vesicles.ion transport was differentiated from superficial ion binding by (a) the time course of association of radioisotope with the vesicles ...197616659668
lack of influence of phytochrome on membrane permeability to tritiated water.the water permeability of tissues was investigated by measuring the efflux of (3)hho from previously loaded (in darkness) etiolated bean buds (phaseolus vulgaris l. var. red kidney), pea epicotyl segments (pisum sativum l. var. alaska), and oat coleoptile segments (avena sativa l. var. garry). red light, far red light, or darkness was applied at the time of transfer of tissue from labeled to unlabeled medium. there were no effects of light on half-time for efflux or on the maximum level of radio ...197616659447
polypeptide binding to plastid envelopes during chloroplast development.pulse-chase experiments using [(35)s]methionine with various light and dark regimes have been used to follow in situ polypeptide accumulation during plastid development in avena sativa (var. mostyn). subsequent isolation and later fraction of the etioplasts, etio-chloroplasts or chloroplasts into envelope, stroma lamellae, thylakoid and supernatant (stromal) fractions has enabled a survey of the movement of label between the different fractions. these studies show that considerable and sometimes ...197624430904
changes in envelope permeability during chloroplast development.the permeability of the plastid envelope during the development of avena sativa plastids was investigated by light scattering and uptake of various labelled compounds (malate, succinate, glutamate, α-ketoglutarate, citrate, glycine, sucrose). the results presented show that a primary event during greening is a change in permeability, thereby allowing an increased transport of metabolites across the membranes of very early etio-chloroplast stages. the results are discussed in view of an adaption ...197624430818
phytochrome control of electrical potentials and intercellular coupling in oat-coleoptile tissue.microelectrodes were used to demonstrate two electrical responses which occur in oat (avena sativa l.) coleoptile parenchyma-cells during exposure to red light. the membrane potential of these cells depolarized 5-10 mv in several seconds in red light and repolarized more slowly in far-red light. by pulsing current through the cells, it was found that cellular coupling along the longitudinal axis of the coleoptile increased about 2-fold in red light, but that coupling along the lateral axis was n ...197624424904
cell elongation in the grass pulvinus in response to geotropic stimulation and auxin application.horizontally-placed segments of avena sativa l. shoots show a negative geotropic response after a period of 30 min. this response is based on cell elongation on the lower side of the leaf-sheath base (pulvinus). triticum aestivum l., hordeum vulgare l. and secale cereale l. also show geotropic responses that are similar to those in avena shoots. the pulvinus is a highly specialized organ with radial symmetry and is made up of epidermal, vascular, parenchymatous and collenchymatous tissues. stato ...197624424826
fluxes of gibberellic and abscisic acids, together with that of adenosine 3',5'-cyclic phosphate, across plastid envelopes during development.application of the technique of silicone oil centrifugation to etioplasts and etio-chloroplasts isolated from greening etiolated avena sativa l. laminae has shown that etioplast envelope membranes are relatively impermeable to gibberellic acid, abscisic acid and adenosine 3',5'-cyclic phosphate. however, there is a marked increase in permeability for all in the early stages of greening, particularly after 2 h. all fluxes are reduced thereafter but a high level of abscisic acid still remains.197624424702
changes in the permeability of the inner mitochondrial membrane associated with plastid development.mitochondria isolated from greening etiolated laminae of avena sativa l. show changes in the permeability of their inner membranes during chloroplast development similar to those described earlier for plastids. oxalo-acetate, succinate and α-keto-glutarate permeate most readily inner membranes of mitochondria isolated from laminae given 2 h illumination whilst glutamate and glycine show later and more general penetration into the matrix spaces of mitochondria from greening tissue. aminolevulinic ...197624424690
movement of labelled metabolites from mitochondria to plastids during development.the exchange of metabolites from isolated mitochondria of avena sativa l. to isolated etioplasts or 1-24 h etio-chloroplasts, from the same laminae, has been investigated. the results confirm the synchronised changes in the permeabilities of the inner membranes of each during plastid development. they also indicated the possibility of directed transport of certain metabolites from mitochondria to plastids especially during the early stages of chloroplast maturation. over 80% of labelled succinat ...197624424689
binding of (45)ca (2+) to particulate fractions of coleoptile tissue.using recently developed techniques, we have investigated the binding of (45)ca(2+) to membrane preparations from corn (zea mays l) and oat (avena sativa l) coleoptile tissue. scatchard plot analysis reveals at least two ca(2+)-binding sites in each tissue, a high affinity binding site (k m=7.7×10(-7) m, n=6.9×10(-10) mol·0.5 g f.w.(-1) in corn, k m=4.93×10(-6) m, n=2.29×10(-9) mol·0.5 g f.w.(-1) in avena) and a low affinity binding site (k m=9.01×10(-5) m, n=5.4×10(-8) mol·0.5 g f.w.(-1) in cor ...197624424643
[light dependence of phytol accumulation. a contribution to the question of chlorophyll biosynthesis].phytol is identified by gas chromatography and mass spectrometry and its concentration determined (range 0.005-3 μg) in darkgrown and irradiated plants. seeds of oats (avena sativa l.), wheat (triticum aestivum l.) and barley (hordeum vulgare l.) contain bound phytol (2-5 μg/g). the phytol content decreases during germination in the dark. phytol synthesis in dark-grown seedlings starts in the light and stops in the dark again. the degradation of phytol in the dark is much slower than that of chl ...197624424592
inhibition of iaa-induced elongation in avena coleoptile segments by lead: a physiological and an electron microscopic study.a high resolution growth measuring apparatus was used to demonstrate the inhibition of auxin-induced cell elongation in oat coleoptile segments (avena sativa l. var holden) by lead at concentrations ranging from 2 x 10-6 m to 2 x 10-3 m. the inhibition was immediate, having no measurable lag period. electron micrographs of lead-treated and control segments revealed that in the treated material, lead became localized as electron-dense granules in the cell walls and in vesicles associated with dic ...19761001018
evidence for ammonium-dependent de novo synthesis of glutamate dehydrogenase in detached oat leaves.glutamate dehydrogenase becomes density labeled through the incorporation of deuterium and (15)n when detached oat leaves (avena sativa var. fulghum) are incubated in the presence of ammonia. the enzyme has been isolated by means of deae-cellulose chromatography, ammonium sulfate precipitation, isopycnic equilibrium centrifugation, and disc electrophoresis from leaves fed l-methionine-(35)s. radioactivity is incorporated into isozyme 1 of glutamate dehydrogenase, whereas isozyme 2, detected only ...197516659411
regulation of cell wall synthesis in avena stem segments by gibberellic acid.gibberellic acid induces (a) increased elongation of avena sativa stem segments, (b) increased formation of cell wall material, measured on the basis of dry weight, and (c) increased incorporation of (14)c-glucose into all fractions of the cell wall material. this increased incorporation of radioactivity correlates well with increased formation of cell wall material and shows a time-course pattern similar to the time course of the elongation response. approximately one hour after the application ...197516659206
the metabolism of oat leaves during senescence: iii. the senescence of isolated chloroplasts.the changes in chlorophyll and protein in senescing chloroplasts isolated from the first leaves of 7-day-old oat (avena sativa) seedlings have been investigated. in darkness the chlorophyll in these plastids is highly stable, losing only 5 to 10% of its content after 7 days at 26 c. this result contrasts with the behavior of chlorophyll in intact leaves, in which about 80% of the pigment would have disappeared in that time. the protein is less stable than the chlorophyll, though more stable than ...197516659176
reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form.the intracellular localization of phytochrome was seen in dark-grown oat (avena sativa l., cv. garry) and rice (oryza sativa l., cv. unknown) shoots after various light treatments using an indirect peroxidase-antiperoxidase antibody labeling method. phytochrome is generally distributed throughout the cytoplasm in cells of tissue that had not been exposed to light prior to fixation. within, at most, 8 min after the onset of saturating red irradiation, phytochrome, now present in the far-red-absor ...19751093170
studies on the presence of adenosine cyclic 3':5'-monophosphate in oat coleoptiles.the incorporation of adenosine-8-(14)c into adenosine cyclic 3':5'-monophosphate in coleoptile-first leaf segments of avena sativa l. was investigated. homogenates of segments incubated in adenosine-8-(14)c for either 4 or 10 hours were partially purified by thin layer chromatography followed by paper electrophoresis. a radioactive fraction, less than 0.06% of the (14)c present in the original homogenate, migrated as adenosine cyclic 3':5'-monophosphate during electrophoresis. upon treatment wit ...197516659080
immunological and physical characterization of the products of phytochrome proteolysis.the relationship between high molecular weight (large) and low molecular weight (small) forms of phytochrome has been shown earlier to be one of proteolysis. the products of such proteolysis are characterized here by chromatography through bio-gel p-200 using specific antiphytochrome sera as an assay system. degradation of large oat (avena sativa l. cv. garry) phytochrome as phytochrome, red-absorbing form, phytochrome, far red-absorbing form, or under cycling conditions in crude preparations co ...197516659053
[light-mediated de-novo synthesis of δ-aminolaevulinate dehydratase in isolated avena etioplasts].a light dependent increase of the activity of δ-aminolaevulinate dehydratase (e.c. 4.2.1.24) in isolated etioplasts of avena sativa l. was shown. this increase can be assumed to be due to a de-novo synthesis of the enzyme. chloramphenicol was found to inhibit this synthesis, whereas cycloheximide did not have any effect. illumination with red light (660 nm) was followed by the same increase of porphobilinogen production as illumination with white light; far-red (731 nm) did not induce such an ef ...197524435263
effects of magnesium, calcium and lanthanum ions on stomatal oscillations in avena sativa l.mg(2+), ca(2+) and la(3+) caused an increased period time of the transpiratory oscillations when given to excised oscillating avena plants (plants without root system). the effect was reversible, i.e. after withdrawal of the ions the period time returned to its original value. in order to achieve the same period lengthening as with 2.5 mm la(3+), 20 mm ca(2+) and 40 mm mg(2+) was needed. the effects are discussed along two lines: (a) the ions interfere with ionic processes, central for the stoma ...197524435179
developmental changes in the surface properties of chloroplast membranes.when the surface properties of etioplast and etiochloroplast membranes from dark grown or variously illuminated leaves of avena sativa l. are examined by counter current distribution in a dextran: poly(ethylene-glycol) two-phase system the results may be summarized as follows: (i) etioplast membranes consist of two main classes with respect to upper phase poly(ethylene-glycol) affinity. (ii) shortly after illumination the proportion of higher affinity membranes increases and the upper phase affi ...197524430083
plasma membrane adenosine triphosphatase of oat roots: activation and inhibition by mg and atp.atpase activity of plasma membrane vesicles isolated from oat (avena sativa l. cv. goodfield) roots was examined in the presence of various concentrations of mgcl(2) and atp. a mg(2+): atp ratio of about 1 was required for maximal activity regardless of the concentrations used; the optimum concentration for both mg(2+) and atp was 9 mm. based on the atpase activity at different concentrations of complexed mg.atp and free atp, it is concluded that mg.atp is the true substrate of this enzyme.under ...197516659034
multiple forms of invertase in developing oat internodes.three different invertases are found in the developing internodes of oat (avena sativa cv. victory). two soluble invertases (i and ii) are separable on diethylaminoethylcellulose and sephadex columns. they are further distinguished by their kinetic constants, heat stability, and differences in stability and apparent activity optima in response to ph treatments. relative activities of the two soluble isozymes change considerably during the developmental stages examined. invertase i activity rises ...197516659014
the limit dextrinases from ungerminated oats (avena sativa l.) and ungerminated rice (oryza sativa l.).the limit dextrinases from ungerminated oats and rice have been purified, and their substrate specificity compared with a bacterial isoamylase preparation. both cereal enzymes could hydrolyse (1 yields6)-alpha-d-glucosidic linkages in oligosaccharide alpha-dextrins, pullulan, amylopectin, and the beta-limit dextrins of amylopectin and glycogen. however, under comparable conditions, they were unable to attack glycogens.19751170015
the pharmacology of avena sativa.the pharmacology of avena sativa has been investigated in laboratory animals following a report that tincture of avena sativa reduced the craying for cigarettes in man. the tincture, evaporated to dryness, craving for cigarettes in man. the tincture, evaporated to dryness, re-constituted in an equal volume of water and administered by stomach tube or intraperitoneal injection, antagonized the antinociceptive effect of morphine in two separate test (hot-plate and tail flick). compared with animal ...1975237083
phytochrome characterization by rabbit antiserum against high molecular weight phytochrome.both small and large sizes of phytochrome purified from garry oat (avena sativa l. ev. garry) as well as large phytochrome purified from newton oat (a. sativa l. cv. newton), rye (secale cereale l. cv. balbo), barley (hordeum vulgare l. cv. harrison), and pea (pisum sativum l. cv. alaska) seedlings are characterized by a specific antiserum against large garry oat phytochrome. a spur is observed by double diffusion assay against large and small garry oat phytochrome indicating only partial identi ...197516659052
use of protein in extraction and stabilization of nitrate reductase.the in vitro instability of nitrate reductase (ec 1.6.6.1) activity from leaves of several species of higher plants was investigated. decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. the rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. activity was relatively stable in certain genotypes of zea mays l., but extremely unstable in others. in all genotypes of avena sativa l. and nicotiana tabacu ...197416658769
lack of effect of avena sativa on cigarette smoking. 19744431518
stimulation of avena coleoptile growth by reduction of oxygen supply.the growth of avena sativa l. coleoptiles was accelerated by reduction of the o2 concentration in the surrounding atmosphere. cell-wall extensibility was increased in close relation to the increase in elongation, 6-8% o2 giving the optimal effect in either case. growth promotion by reduced o2 concentration and by auxin (indole-3-acetic acid; iaa) were additive, at least at lower auxin concentrations.this response to reduced o2 concentration was also present in sections pretreated with cyclohexim ...197424458929
effect of ph and auxin on chloride uptake into avena coleoptile cells.the effect of ph on (36)cl(-) movement into coleoptile cells (avena sativa l. cv. garry) was investigated and compared with effects of indoleacetic acid. (36)cl(-) uptake, but not efflux, is stimulated when coleoptile sections are placed in media adjusted to ph levels from 5 to 3 after a preincubation period at ph 6.5. the enhancement is seen within 2 minutes, is not correlated with growth, and is completely erased by respiratory inhibitors. in comparison to the acid-induced stimulation, the sti ...197416658985
iron requirement and iron uptake from various iron compounds by different plant species.the fe requirements of four monocotyledonous plant species (avena sativa l., triticum aestivum l., oryza sativa l., zea mays l.) and of three dicotyledonous species (lycopersicum esculentum mill., cucumis sativus l., glycine maxima (l.) merr.) in hydroponic cultures were ascertained. fe was given as nafe-eddha chelate (fe ethylenediamine di (o-hydroxyphenylacetate). i found that the monocotyledonous species required a substantially higher fe concentration in the nutrient solution in order to att ...197416658933
phytochrome: immunocytochemical assay of synthesis and destruction.the protein moiety of phytochrome, when assayed by an immunocytochemical technique, was not detected in dry oat (avena sativa l., cv. garry) and rye (secale cereale l., cv. balbo) grains or in grains hydrated 4 h at 25°. after 24 h of hydration immunochemical activity of phytochrome in situ was readily detectable. immunocytochemical assays of destruction show that different regions of 3-day-old, single oat and rye seedlings have different destruction kinetics. oat roots apparently exhibit much s ...197424442499
evidence against the occurrence of adenosine-3':5'-cyclic monophosphate in higher plants.previous reports on the incorporation of [(14)c]adenine into adenosine-3':5'-cyclic monophosphate (cyclic amp) in oat (avena sativa l.) and maize (zea mays l.) coleoptile sections, chick-pea (cicer arietinum l.) embryos and barley (hordeum vulgare l.) aleurone layers were reexamined. separation of labelled nucleotides on deae-sephadex a 25 showed that a peak of (14)c activity, previously considered to be cyclic amp, is not identical with this compound. attempts to detect the cyclic nucleotide by ...197424442328
the metabolism of oat leaves during senescence: i. respiration, carbohydrate metabolism, and the action of cytokinins.when the detached first leaves of green or etiolated oat (avena sativa cv. victory) seedlings senesce in the dark, their oxygen consumption shows a large increase, beginning after 24 hours and reaching a peak of up to 2.5 times the initial rate by the 3rd day. this effect takes place while the chlorophyll of green leaves, or the carotenoid of etiolated leaves, is steadily decreasing. kinetin, at a concentration which inhibits the decrease in pigment, completely prevents the respiratory rise; ins ...197416658877
effect of red light on coleoptile growth.the effects of red light in reducing the growth of the oat (avena sativa l.) coleoptile and the synthesis of auxin in the coleoptile tip are detectable 2 hours after treatment and become more pronounced with time. when the coleoptile tip is supplied with additional tryptophan the synthesis of auxin is doubled both in darkness and when exposed to red light. treatment of the tip with gibberellic acid or pyridoxal phosphate overcomes the reduction of auxin synthesis caused by red light. the uptake ...197416658875
concentrations of indole-3-acetic acid and its esters in avena and zea.an isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. application of this method to seedlings of avena sativa and zea mays indicates the upper limit of free indole-3-acetic acid in avena to be about 16 mug per kg and in zea, about 24 mug. the amount of 1 n alkali-labile indole-3-acetic acid in zea is about 330 mug per kg and there is very little 1 n alkali- ...197416658870
effects of indoleacetic acid on dictyosomes of apical and expanding cells of oat coleoptiles.we found that the auxin-induced growth is mediated through the activation of the dictyosomes (collectively, the golgi apparatus). incubation of oat (avena sativa) coleoptile segments in indoleacetic acid-sucrose-phosphate buffer changes significantly the number of dictyosomes in the expanding cells. a further indication of auxin enhancement of dictyosome activity is a decrease in dictyosomal cisternae (flattened membranous sacs) number. this decrease occurred after 6 minutes of incubation in aux ...197416658863
proceedings: on the pharmacology of an extract of avena sativa. 19744451796
letter: treatment of nicotine addiction with avena sativa. 19744471694
multiple forms and intracellular localization of uridine diphosphate glucose pyrophosphorylase in avena sativa.uridine diphosphate glucose pyrophosphorylase was isolated separately from avena sativa leaves, roots, and etiolated coleoptiles and purified by ammonium sulfate fractionation, deae-sephadex chromatography and polyacrylamide gel electrophoresis. there was no difference in the enzyme from the different tissue types with respect to properties exhibited during the purification procedure. a small portion of the enzyme from all three sources was found to be particulate when homogenized in aqueous suc ...197416658857
the influence of 0.03% carbon dioxide on protein metabolism of etiolated avena sativa coleoptiles.the influence of indoleacetic acid, 0.03% co(2), and malate on protein metabolism of etiolated avena sativa coleoptile sections has been investigated. all three were found to elevate both the rate of incorporation of labeled leucine into protein, and the level of soluble protein. the combination of indoleacetic acid and co(2) stimulated these values in an additive or weakly synergistic manner, in contrast to the nonadditive influence of malate and co(2). evidence is presented that cyclo-heximide ...197416658830
a synergistic stimulation of avena sativa coleoptile elongation by indoleacetic acid and carbon dioxide.the ability of 0.03% co(2) to stimulate growth has been investigated using etiolated avena coleoptile sections maintained in buffered solution. this concentration of co(2)-stimulated growth after a lag period of 12 to 15 minutes, and a synergistic relationship between indoleacetic acid and co(2) in stimulating growth has been demonstrated. the response to co(2) is inhibited by cycloheximide and is lost approximately 10 minutes after exposure to co(2)-free air. malate can replace co(2) in stimula ...197416658829
the effect of abscisic acid on the uptake of potassium and chloride into avena coleoptile sections.the effect of abscisic acid (aba) on uptake of potassium ((86)bb(+) or (42)k(+)) by avena sativa l. coleoptile sections was investigated. aba lowered the potassium uptake rate within 30 min after its application and inhibition reached a maximum (ca. 75%) after 2 h. the inhibition of k(+) uptake increased with aba concentration over a range of 0.03 to 10 μg/ml aba. at a higher k(+) concentration (20 mm) the percentage inhibition decreased. the percentage inhibition of k(+) uptake by aba remained ...197424458128
the use of an enzyme electrode in the analysis of indole-3-acetic acid oxidase activity in avena.a flexible analytical system which allows for the continuous potentiometric monitoring of the disappearance of an electrochemical species, ferrocyanide, by the peroxidase enzyme is described. the ability of peroxidase to mediate the oxidation of indole-3-acetic acid is followed by observing the competition of indole-3-acetic acid with ferrocyanide for the peroxidase enzyme. this is accomplished by examining potentiometrically the decrease in the rate of ferrocyanide oxidation with increasing ind ...197416658775
amyloplast size and number in gravity-compensated oat seedlings.gravity compensation by the horizontal clinostat increases the diameter of amyloplast starch grains of oat (avena sativa cv. victory) coleoptile parenchyma cells, as compared to vertically rotated and stationary controls. in dark-grown coleoptile tip parenchyma cells, measured starch grain sizes exhibit a wide distribution of diameters, from approximately 1.5 to approximately 8.0 mum, but fall into three prominent diameter classes. the compensated tissues from both the tip and the subapical regi ...197416658713
phytochrome stability in vitro: i. effect of metal ions.photoreversible phytochrome disappears from etiolated tissue upon actinic irradiation. such disappearance, of possible physiological importance, involves several processes, at least one of which is accelerated by metals in vivo. purified phytochrome from oat (avena sativa l. cv. garry) coleoptiles is greatly stabilized in vitro by scrupulous removal of metal impurities via chelating agents. such stabilized phytochrome decays rapidly upon the addition of about 10 mum hg(2+), cd(2+), cu(2+), and z ...197416658705
activation of avena coleoptile cell wall glycosidases by hydrogen ions and auxin.several cell wall-bound glycosidases present in avena sativa coleoptiles were assayed by following the hydrolysis of p-nitrophenyl-glycosides. particular emphasis was placed on characterizing some parameters affecting the activity of beta-galactosidase. the ph optimum of this enzyme is 4.5 to 5.5; it is sensitive to copper ions and p-chloromercuribenzoate treatment and apparently has an exceptionally low turnover rate. indoleacetic acid treatment enhanced in vivo beta-galactosidase activity of c ...197416658680
changes in plastid envelope polypeptides during chloroplast development.a quantitative estimation of sodium dodecyl sulphate-extractable plastid envelope polypeptides during greening of avena sativa l. laminae is described, combined with protein distribution and plastid number studies over the same period.a primary light-dependent and cytoplasm-dependent increase in both total plastid protein and envelope associated protein during the first 30 minutes of greening was observed, followed by a period during which release of envelope-associated protein either into the p ...197424442806
iaa transport during the phototropic responses of intact zea and avena coleoptiles.transport of indolyl-3-acetic acid (iaa) was studied during the phototropic responses of intact shoots and detached coleoptiles of zea mays l. and avena sativa l. the use of a high specific activity [5-(3)h]iaa and glass micropipettes enabled asymmetric application of the iaa to be made to individual coleoptiles with minimal tissue damage.a unilateral stimulus of 2.59×10(-11) einstein cm(-2) of blue light, probably in the dose range of the first positive phototropic response, caused significant ...197424442803
subcellular localization of the red-absorbing form of phytochrome by immunocytochemistry.an immunocytochemical technique was used to localize the red-absorbing form of phytochrome at the light- or electron-microscope level in etiolated barley (hordeum vulgare l.) coleoptile tip, rice (oryza sativa l.) coleoptilar node, maize (zea mays l.) coleoptile tip, rye (secale cereale l.) coleoptile tip and coleoptilar node, and oat (avena sativa l.) root cap. staining for phytochrome in the cells was found to be generally distributed throughout the cytoplasm. in addition, barley also showed s ...197424442776
phytochrome destruction: an apparent requirement for protein synthesis in the induction of the destruction mechanism.examination of the phytochrome destruction reaction as a function of age in etiolated oat (avena sativa l. cv. garry) seedlings demonstrates that following illumination of 3-day-old shoots there is a lag, not observed in 4- or 5-day-old oats, prior to the onset of destruction. this light-mediated induction of the phytochrome destruction mechanism in 3-day-old shoots is inhibited by chloramphenicol, actinomycin d, and puromycin suggesting that protein synthesis is required. in 4-day-old shoots, a ...197316658553
production of aneuploids in avena sativa l. 19734762793
polarity and rate of transport of cyclic adenosine 3,5'-monophosphate in the coleoptile.transport of tritiated cyclic amp in the coleoptile of oats (avena sativa) and corn (zea mays) is polar, with basipetal to acropetal ratios of 4.0 and 3.2, respectively. the rate of transport is approximately that of indoleacetic acid. the linear velocity of transport, however, is at least five times that of auxin. a loss in transport polarity of the nucleotide occurs in subapical tissues within several hours after decapitation of the coleoptile, accompanied by a decrease in transport rate. the ...197316658508
characterization of plasma membrane-associated adenosine triphosphase activity of oat roots.atpase activity of plasma membranes isolated from oat (avena sativa l. cv. goodfield) roots was activated by divalent cations (mg(2+) = mn(2+) > zn(2+) > fe(2+) > ca(2+)) and further stimulated by kcl and a variety of monovalent salts, both inorganic and organic. the enzyme exhibited greater specificity for cations than anions. the presence of mg(2+) was necessary for kcl stimulation. ca(2+) was ineffective in replacing mg(2+) for activation of plasma membrane atpase, but it did activate other m ...197316658500
the lateral transport of iaa in intact coleoptiles of avena sativa l. and zea mays l. during geotropic stimulation.movement of iaa was studied in excised coleoptile apices and whole seedlings of zea mays l. and avena sativa l. during geotropic stimulation. a micropipette technique permitted the application of [5-(3)h]iaa at predetermined points on the coleoptiles with minimal tissue damage.when [5-(3)h]iaa was applied to the upper side of a horizontal excised zea coleoptile, about 60% of the recoverable radioactivity had moved into the lower half after 2 h. in contrast, when application was made to the lower ...197324458860
substrate activation of beta-(1 --> 3) glucan synthetase and its effect on the structure of beta-glucan obtained from udp-d-glucose and particulate enzyme of oat coleoptiles.udp-d-glucose, at a micromolar level in the presence of mgcl(2) and oat (avena sativa) coleoptile particulate enzyme which contains both beta-(1 --> 3) and beta-(1 --> 4) glucan synthetases, produces glucan with mainly beta-(1 --> 4) glucosyl linkages. an activation of beta-(1 --> 3) glucan synthetase by udp-d-glucose and a decrease in the formation of beta-(1 --> 3) glucan in the presence of mgcl(2) have been observed. however, at high substrate concentration (>/= 10(-4)m), the activation of be ...197316658488
on the nature of the physiological responses of avena stem segments to gibberellic acid treatment.gibberellic acid was found to cause elongation in avena sativa (oat) stem segments whether it was applied continuously or as a short pulse. the shorter the pulse time became, the higher was the gibberellic acid concentration needed to cause elongation; the segmental growth apparently depends upon the amount of gibberellic acid taken up by the segments. avena segments showed a decreased growth response to gibberellic acid if the treatments were initiated at increasingly later times after excision ...197316658458
somatic association of chromosomes in asynaptic genotypes of avena sativa l. 19734712858
inhibition of low ph-induced elongation in avena coleoptiles by abscisic acid.an angular position-sensing transducer was used to make continuous measurements of acid-induced elongation of avena sativa coleoptile segments. elongation rates at ph 4.5 (5 mm succinate buffer) were about 5-fold greater than those at ph 6.0. buffered 0.1 mm abscisic acid produced a partial decrease of the growth rate. pretreatments with abscisic acid buffered at ph 6.0 usually caused a further reduction of the elongation response when the coleoptile segments were subsequently placed in buffer a ...197316658443
purification of oat and rye phytochrome.a purification procedure employing normal chromatographic techniques is outlined for isolating phytochrome from etiolated oat (avena sativa l.) seedlings. yields in excess of 20% (25 milligrams or more) of phytochrome in crude extract were obtained from 10- to 15-kilograms lots. the purified oat phytochrome had an absorbance ratio (a(280) nm/a(665) nm) of 0.78 to 0.85, comparable to reported values, and gave a single major band with an estimated molecular weight of 62,000 on electrophoresis in s ...197316658440
membrane-bound adenosine triphosphatase activities of oat roots.homogenates of oat (avena sativa cv. goodfield) roots contained at least five membrane-associated adenosine triphosphatase (atpase) activities. the membrane-bound atpases were separated on sucrose gradients and distinguished by membrane density, ph optima, sensitivity to monovalent salts, and substrate specificity.a membrane fraction sedimenting at low centrifugal force (13,000g) contained two atpase activities at ph 9.0. one membrane atpase was coincident with cytochrome c oxidase activity and ...197316658403
rhythmicity in the basipetal transport of indoleacetic acid through coleoptiles.(14)c-indoleacetic acid was applied to coleoptiles of corn (zea mays) and oat (avena sativa). the coleoptiles were detached from the endosperms at 6-minute intervals after indoleacetic acid application, and the radioactivity was determined in successive 2-millimeter regions. the rate (per cent per minute) of basipetal transport of indoleacetic acid is periodic in various regions of the coleoptile, with a period of about 20 minutes. the possible relation of this cyclic phenomenon to other rhythmi ...197316658381
kinetics of stress relaxation properties of oat coleoptile cell wall after geotropic stimulation.this study describes the stress relaxation of the cell wall of oat (avena sativa) coleoptiles after different periods of geotropic stimulation. the upper and lower tissues (with respect to gravity) of geotrophically stimulated coleoptiles exhibit different wall properties. the lower tissues are less resistant to deformation than the upper. the ratio of stress to strain is significatly less in the lower than in the upper tissue. similarly, the relaxation time and the minimum relaxation time, deri ...197316658352
rapid growth inhibition of avena coleoptile segments by abscisic acid.an angular position sensing transducer was used to make continuous measurements of elongation of a column of avena sativa coleoptile segments. elongation stimulated by 2 mum indoleacetic acid was inhibited by 0.1 mm abscisic acid with a latent period of about 4 or 5 minutes at ph 6.0, 30 c. full growth inhibition was not established until about 1 hour after the addition of the abscisic acid. the same degree of final growth inhibition could be obtained under the above conditions using 10 mum and ...197316658304
comparative immunochemistry of phytochrome.partially purified high molecular weight preparations of phytochrome, estimated to be close to 440,000 molecular weight based upon chromatography through a calibrated bio-gel p-300 column, were obtained from garry and newton oats (avena sativa l., cv. garry and cv. newton), rye (secale cereale l., cv. balbo), barley (horedum vulgare l., cv. harrison), and pea (pisum sativum l., cv. alaska) by a sequence of three chromatographic steps: brushite, diethylaminoethyl cellulose, and bio-gel p-300. no ...197316658285
electrical properties of parenchymal cell membranes in the oat coleoptile.parenchymal cells of oat (avena sativa) coleoptiles had an osmotic concentration of 410 mm (determined by plasmolysis); of this only 22 mm was k(+) and 1 mm na(+) (flame photometry). cells were impaled with micropipette electrodes. iontophoretic injection of the dye niagara sky-blue from the micropipette showed that the tip of the electrode penetrated the vacuole. when sections of tissue were immersed in a solution of 22 mm kcl, 1 mm cacl2, and 50 mm glucose, average membrane potential was found ...197224482272
aspects of phytochrome decay in etiolated seedlings under continuous illumination.the rate of total phytochrome decay in the dicotyledons amaranthus caudatus, mirabilis jalapa and pisum sativum under continuous illumination with red, incandescent, and blue light depends on the pfr/ptotal maintained by each source. amaranthus is an exception to this in that there is a deviation from firstorder decay kinetics under continuous illumination with incancdescent light. this deviation is probably not related to the chlorophyll present in the amaranthus sample since chlorophyll-rich p ...197224482270
auxin-induced changes in avena coleoptile cell wall composition.sugar and uronic acid residues were derived from wall polysaccharides of oat (avena sativa, var. victory) coleoptiles by means of 2 n trifluoroacetic acid, 72% sulfuric acid, or enzymic hydrolysis. the products of hydrolysis were reduced and acetylated to form alditol acetates which were analyzed using gas chromatography. time-course studies of auxin-promoted changes in various wall fractions indicate that when exogenous glucose was available, increases in certain wall constituents paralleled in ...197216658216
purification of an ion-stimulated adenosine triphosphatase from plant roots: association with plasma membranes.a membrane-bound adenosine triphosphatase (ec 3.6.1.3) that requires mg(++) and that is stimulated by monovalent ions has been purified 7- to 8-fold from homogenates of oat (avena sativa l. cult. goodfield) roots by discontinuous sucrose-gradient centrifugation. the enzyme was substrate specific; adenosine triphosphate was hydrolyzed 25 times more rapidly than other nucleoside triphosphates. the membrane fraction containing adenosine triphosphatase was enriched in plasma membranes, which were id ...197216592027
photoregulation of nicotinamide adenine dinucleotide kinase activity in cell-free extracts.this article gives evidence that nad kinase activity is controlled by the action of phytochrome. the nadp level rapidly increased in the cotyledons of seedlings of pharbitis nil strain violet (a short day plant), when the inductive dark for flowering was interrupted with a 5-minute illumination of red light. illumination with far red light immediately after illumination with red light counteracted partly the effect of the latter.a partially purified phytochrome preparation from the uppermost int ...197216658195
role of protein synthesis in the senescence of leaves: ii. the influence of amino acids on senescence.when the first leaf of the oat (avena sativa) seedling is detached and placed in the dark, yellowing and proteolysis take place rapidly. the earlier finding that d-serine promotes this process has led to a further study of the controlling roles of several amino acids. since the action of serine was found to be more powerful in presence of kinetin than alone, the effects of other amino acids have been restudied in presence of kinetin. cysteine emerges as a moderately strong promotor of senescence ...197216658191
[experiments on the localisation of flavonoids in plastids. i. flavonoids in etioplasts of avena sativa l]. 19724405256
intracellular distribution of mitochondria after geotropic stimulation of the oat coleoptile.the number of mitochondria is greater in the bottom than in the top of cells in geotropically stimulated oat (avena sativa) coleoptiles. in the avascular tip and outer epidermis of subapical regions this difference occurs only in the lower tissues. these inequalities are found both in the kmno(4) (-) and in the glutaraldehyde-fixed tissues; however, they are significant only in the former. also, the number of mitochondria scored is consistently lower when the tissues were fixed in kmno(4). these ...197216658131
isolation of protoplasts from cereal leaves.mature leaves of secale cereale cut into narrow strips and incubated for 18 h in a mixture of cellulase (meicelase) and pectinase (pectinol r10) produced quantities of protoplasts. under the same conditions leaves of triticum aestivum, hordeum vulgare and avena sativa also produce protoplasts but in lower yields. the wheat and rye protoplasts in culture appear to regenerate a cell wall but only a very small proportion undergo cell division.197224481701
distribution and activation of the golgi apparatus in geotropism.we find a differential distribution of dictyosomes in the avascular tip cells of the oat (avena sativa) coleoptile upon geostimulation. a differential activation (increased vesicle production) of the dictyosomes with respect to gravity also occurs, but only in the tip cells of the lower tissues. similar differences in distribution and activation of dictyosomes occur also in cells subjacent to the avascular tip (1st and 5th millimeter from the apex) of both the upper and lower half-tissues. when ...197216658015
phosphorylated and nucleotide sugar metabolism in relation to cell wall production in avena coleoptiles treated with fluroride and peroxyacetyl nitrate.coleoptile sections of avena sativa l. were pretreated with sodium fluoride or peroxyacetyl nitrate at levels which inhibit auxin-induced growth but did not affect glucose uptake or co(2) production when postincubated for 30 minutes in a (14)c-glucose medium without auxin. labeling of metabolites involved in cell wall synthesis was measured. peroxyacetyl nitrate decreased labeling, and it was concluded that the pool size of uridine di-phosphoglucose, sucrose, and cell wall polysaccharides decrea ...197216657997
the dark reactions of rye phytochrome in vivo and in vitro.the dark reactions of secale cereale l. cv. balbo phytochrome have been investigated in coleoptile tips and in extensively purified extracts of large molecular weight phytochrome. destruction, but not reversion, was detected in vivo. the effects of various inhibitors of an in vitro phytochrome-degrading protease did not support a view of proteolytic attack as the basis of in vivo destruction. in vitro, rye phytochrome (about 240,000 molecular weight) reverted extremely rapidly, even at 5 c. the ...197216657993
photoconversion of riboflavin to lumichrome in plant tissues.free flavins have been extracted from shoots of etiolated corn (zea mays l., var. burpee snowcross) and from yeast cells and separated from other substances by absorption on resorcinol-formaldehyde resin and talc columns and by thin layer chromatography. riboflavin was the only free flavin present. extracts of etiolated shoots of oats (avena sativa l., var. multiline e-69 and clinford) yielded riboflavin plus a second free flavin previously demonstrated in oats. the areas of the chromatograms ex ...197216658098
"disaggregation" of phytochrome in vitro-a consequence of proteolysis.the relationship between a large molecular weight (9s) and a small molecular weight (4.5s, 60,000 molecular weight) species of phytochrome was examined to determine if the larger species was an aggregate of the smaller. alterations of ph, salt concentration, or phytochrome concentration did not cause any observable formation of the large form from the small form. however, in partially purified phytochrome extracts from secale cereale l. and avena sativa l., the large form was converted to the sm ...197116657862
effect of avena sativa on cigarette smoking. 19714939551
influence of temperature and seed ripening on the in-vivo incorporation of co(2) into the lipids of oat grains (avena sativa l.).to elucidate the influence of growth temperature and of stage of maturity on lipid synthesis in seeds, oat plants (avena sativa nuda l., variety nos) were fed with (14)co(2) at different stages after flowering, and the (14)c-incorporation into the grain lipids was determined at 2, 24, and 48 hours after the end of (14)co(2)-application. by changing growth temperature from 12 c to 28 c after the application of (14)co(2) to intact plants, a higher (14)c-labeling of saturated fatty acids was found ...197116657814
[differences in the light-activation of nadp-dependent glyceraldehyde-3-phosphate dehydrogenase and of ribulose-5-phosphate kinase between plants containing the calvin and those containing the c4-dicarboxylic acid pathway of photosynthetic carbon reduction].1. preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of nadp-gpd (ziegler and ziegler, 1965) as well as of ribulose-5-phosphate kinase (latzko and gibbs, 1969). examination of several species which carry out the calvin type of photosynthetic co2 fixation (vicia faba, spinacia oleracea, nicotiana tabacum, avena sativa) now revealed that the dark level of nadp-gpd activity ranges between 300-400 μmol nadph/mg c ...197124493083
solubilization and separation of uridine diphospho-d-glucose: beta-(1 --> 4) glucan and uridine diphospho-d-glucose:beta-(1 --> 3) glucan glucosyltransferases from coleoptiles of avena sativa.the particulate glucan synthetase preparation isolated from a homogenate of oat coleoptiles at 4 c lost 65% of its original activity after 1 day when the udp-d-glucose substrate concentration was 5 x 10(-7)m to 1.0 x 10(-6)m. storage of the particulate enzyme at -20 c or in liquid nitrogen did not prevent the enzyme from losing its activity. incorporation of 0.5% hovine serum albumin into the medium stabilized the particulate enzyme at 0 c for 6 days and for at least 2 weeks in liquid nitrogen.w ...197116657697
induction of coleoptile elongation by carbon dioxide.the ability of co(2) to induce elongation of avena sativa coleoptile segments was examined with the use of a high resolution growth-recording device. co(2)-saturated water causes an 8- to 16-fold promotion in the rate of elongation within 1 minute. this elongation is insensitive to a variety of metabolic inhibitors that suppress auxin-induced elongation, and the co(2) effect cannot be prevented by pretreatment with these inhibitors. buffers of ph 3 to 4 also stimulate elongation quickly, and it ...197116657618
evidence for an electrogenic ion transport pump in cells of higher plants.cyanide (cn) and dinitrophenol (dnp) rapidly depolarize the cells of oat coleoptiles (avena sativa l., cultivar victory) and of pea epicotyls (pisum sativum l., cultivar alaska); the effect is reversible. this indicates that electrogenesis is metabolic in origin, and, since active transport is blocked in the presence of cn and dnp, perhaps caused by interference with atp synthesis, that development of cell potential may be associated with active ion transport. additional evidence for an electrog ...197024174194
studies on phytochrome. some properties of electrophoretically pure phytochrome.1. phytochrome was purified from etiolated oat (avena sativa) seedlings either by gel-filtration chromatography and ion-exchange chromatography or by gel-filtration chromatography and calcium phosphate chromatography. differences were observed in the spectral properties of phytochrome isolated by the two methods. 2. electrophoresis of pure phytochrome at ph values between 9.0 and 6.0 showed the tendency of phytochrome to form different molecular species. studies in the ultracentrifuge did not sh ...19705499974
compartments and fluxes of k, na, and cl in avena coleoptile cells.by the compartmental analysis method of macrobbie and dainty, and pitman, estimates of k(+), na(+), and cl(-) concentrations and fluxes were obtained for the cytoplasm and vacuole of coleoptile cells of oat, avena sativa l. cv. victory. double labeling was used in experiments with (42)k plus (22)na and with (42)k plus (36)cl in a complete nutrient solution. at the plasmalemma, according to the ussing-teorell flux ratio equation, na(+) is pumped out and cl(-) is actively transported inward. the r ...197016657527
auxin transport in avena: i. indoleacetic acid-c distributions and speeds.measurements have been made on the initial stages of the transport of carbon-14-labeled indoleacetic acid in the coleoptile of avena sativa l. concentrations of mobile and immobilized indoleacetic acid are related to both distance and time during the first 2 hours after application of the indoleacetic acid at several concentrations to the top of the decapitated coleoptile.at the lowest concentration of indoleacetic acid applied (0.3 mum), the graphs of concentration against distance are linear f ...197016657448
somatic association in avena sativa l.root tip cells of hexaploid oats avena sativa l. were examined at mitotic metaphase, and distances between homologous as well as between non-homologous chromosomes were measured and their frequency distributions compared. nonhomologous chromosomes were scattered at random in the cells studied. in contrast, the mean distance between homologous chromosomes was significantly shorter. there is a tendency this species. for somatic association of homologs in this species.197017749619
dextranase activity in coleoptiles of avena.ani enzyme activity similar to that of dextranase is associated with coleoptiles of avena sativa. when subjected to purified dextranase, both the pure natural dextran and the cell walls of the avena coleoptiles yield isomaltose and isomaltotriose. thus, the cell walls contain dextrans or dextran-like compounds. coleoptiles with low auxin content have a lower dextranase activity than coleoptiles with high auxin content. the enzyme activity is therefore in some way sensitive to thle hormone.197017742615
determination of linkages of beta-d-glucans from lupinus albus and avena sativa by exo-beta-(1 --> 3)-d-glucanase. 197016657310
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