Publications

gardnerella vaginalis in the urinary tract: incidence and significance in a hospital population.	the significance of gardnerella vaginalis in urine was studied by comparing urine culture results, urinalysis data, and clinical findings. over a two-year period, g vaginalis was reported in 2.3% of all urine cultures. of 72 patients with pure cultures (greater than 10(4) cfu/ml), 43 patients (59.7%) were found to have g vaginalis urinary tract infections. furthermore, four of the infected patients had pyelonephritis. symptoms associated with g vaginalis urinary tract infections varied, and pyur ...	1988	3257296
evaluation of a fluorescent monoclonal antibody reagent for identification of cultured neisseria gonorrhoeae.	we evaluated a new fluorescent monoclonal antibody reagent for confirmation of identity of neisseria gonorrhoeae. the reagent correctly identified all 161 fresh clinical isolates of n. gonorrhoeae, which included 11 penicillinase producing strains (ppng). the reagent also correctly identified 21 stored ppng strains. no cross reactions were seen with 58 fresh clinical isolates of n. meningitidis, 12 stored strains of n. lactamica, or with strains of gardnerella vaginalis, lactobacilli, candida sp ...	1988	3133479
prevalence of lower genital tract infections in pregnancy.	a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ...	1988	3128883
the in-vitro activity of pd127,391, a new quinolone.	mics of pd127,391 a new 4-quinolone, and of ci934 and ciprofloxacin, two previously reported 4-quinolones, were determined for common clinical bacterial isolates by an agar-dilution method. pd127,391 was the most active drug against enterobacteriaceae and acinetobacter spp (mics less than 0.12 mg/l) and as active as ciprofloxacin against aeromonas spp. (mics less than 0.008 mg/l) and pseudomonas aeruginosa (mics less than 1 mg/l). it was more active than ciprofloxacin against pseudomonas spp. in ...	1988	3182416
microbiological profile of the cervix in 1,000 sexually active women.	one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ...	1988	3148302
lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology.	we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ...	1988	3135608
neonatal meningitis due to gardnerella vaginalis.	a case of meningitis due to gardnerella vaginalis occurred in a five-day-old newborn who had clinical signs of fever, polypnea and a grey complexion. after treatment with ampicillin, cefotaxime and netilmicin, the patient's condition improved, and no sequelae were observed. the bacterium isolated from a pure culture of the cerebrospinal fluid was identified by biochemical characteristics to be gardnerella vaginalis, but it was not possible to define the source and mode of contamination.	1988	3137044
gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra.	in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ...	1988	3140532
the cervical cap.	the us food and drug administration has approved marketing of the prentif cavity-rim cervical cap. this contraceptive device is being distributed in the us and canada by cervical cap ltd, los gatos, california. the prentif cap is available in 4 sizes: 22, 25, 28, and 31 mm inside diameter, with a length of 1 1/4-1 1/2 inches. in a multicenter trial involving 522 diaphragm users and 581 cap users followed for 2 years, the cap was 82.6% effective and the diaphragm was 83.3% effective in preven ...	1988	3050407
bacterial vaginosis: a double-blind randomized trial of the effect of treatment of the sexual partner.	in a double-blind randomized controlled trial we assessed the effect of metronidazole treatment of the male partner on the recurrence rate of bacterial vaginosis. women who fulfilled the diagnostic criteria for bacterial vaginosis were treated with metronidazole given in single doses of 2 g on days 1 and 3. the sexual partners were randomized to receive either the same dosage of metronidazole or a placebo. a total of 107 pairs completed the study. one week after the start of treatment 89% of the ...	1988	3056506
trospectomycin, a novel spectinomycin analogue: antibacterial activity and preliminary human pharmacokinetics.	trospectomycin (tsp; u-63366f) is a novel spectinomycin (sp) analogue with broad-spectrum antibacterial activity. the in vitro activity of the analogue was compared to that of sp against approximately 400 bacterial isolates. the in vivo activity of the compound was assessed using experimental infection models for both gram-positive and gram-negative facultative bacteria. the preliminary human pharmacokinetics of tsp were evaluated following single-dose i.v. or i.m. administration. tsp was more a ...	1988	2975212
[in vitro effect of nonoxynol-9 on herpes virus 1 and 2 and on other sexually transmitted micro-organisms].		1988	2854228
a randomized study of antibiotic therapy in idiopathic preterm labor.	a randomized study was undertaken to test the effects of antibiotics as an adjunct to established methods of tocolysis. one hundred fifty patients with cervical dilation of 1 cm or more and no clinically identifiable cause for preterm labor qualified for the study. fifty-three (35%) received 500 mg ampicillin orally every 6 hours, 50 (33%) received 500 mg erythromycin orally every 6 hours, and 47 served as controls. antibiotics were prescribed for 10 days, and the treatment was not altered by th ...	1988	3186088
[genital infections and the course of pregnancy: a prospective study].	the reported study investigates the relationship of genital infections, pathobiochemical findings and demographic data to preterm labor, premature rupture of membranes (prom) and premature delivery. the predictability of chorioamnionitis, puerperal and neonatal infections by these parameters was evaluated concurrently. 301 patients were included in this study between july 1985 and june 1986. 147 of these patients were studied longitudinally during pregnancy, delivery and puerperium (longitudinal ...	1988	3215443
descriptive light and electron microscopy of normal and clue-cell-positive discharge.	in women with clue-cell-positive discharge (ccpd), light-microscopical examination of the wet mount suggests a preference of bacteria for certain vaginal epithelial cells (vecs). to investigate this further, a light- and electron-microscopical study of patients and healthy controls was performed, with special emphasis on vitality and glycogen content of vecs and bacterial-epithelial cell interaction. our study did not reveal morphologic differences between vecs of patients and controls. there wa ...	1988	3257743
diagnosis and clinical manifestations of bacterial vaginosis.	among 640 randomly selected women who were attending a sexually transmitted disease clinic and did not have trichomoniasis, 33% had bacterial vaginosis as defined by a composite of four clinical criteria: (1) vaginal discharge was homogeneous; (2) vaginal discharge had a ph greater than or equal to 4.7; (3) vaginal discharge had an amine-like odor when mixed with 10% potassium hydroxide; (4) vaginal discharge contained clue cells representing greater than or equal to 20% of vaginal epithelial ce ...	1988	3259075
[anaerobic vaginosis].	the incidence of sexually transmitted diseases has increased in recent years. anaerobic vaginosis, caused by gardnerella vaginalis and anaerobic bacteria after subversion of the normal vaginal flora has only recently been the subject of specific studies using improved laboratory techniques.	1988	3259299
a case-control study of chorioamnionic infection and histologic chorioamnionitis in prematurity.	to study the role of infection in prematurity, we studied the demographic and obstetrical characteristics, chorioamnionic cultures, and placental histologic features of women who delivered prematurely and compared these findings with those in women who delivered at term. microorganisms were isolated from the area between the chorion and the amnion (chorioamnion) in 23 of 38 placentas (61 percent) from women with preterm labor who delivered before 37 weeks' gestation and in 12 (21 percent) of 56 ...	1988	3262199
[gardnerella vaginalis in relation to the clinical syndrome of bacterial vaginosis].		1988	3262432
[gardnerella vaginalis in relation to the clinical syndrome of bacterial vaginosis].		1988	3264219
gardnerella vaginalis: diagnosis and management.		1988	3267158
bacterial vaginosis in virginal and sexually active adolescent females: evidence against exclusive sexual transmission.	sixty-eight sexually active and 52 virginal asymptomatic adolescent girls were evaluated for bacterial vaginosis and various laboratory indicators of bacterial vaginosis in a 3-month observational noninterventive study. sixteen of 120 (13%) subjects had bacterial vaginosis during the study. there was no significant difference in the prevalence of bacterial vaginosis or in the prevalence of isolation of gardnerella vaginalis between the sexually active and virginal groups. only a few cases of bac ...	1988	3259076
[clinical evaluation of combined use of the spermicide nonoxynol-9 and condoms].	condoms represent an efficient family planning method. their use was 1st described by the italian anatomist fallopio in 1564. their effectiveness is considered to result in 0.5-2 births/100 years of marriage. 1st descriptions of vaginal contraceptives, by the egyptians, date back to 1850 b.c. their effectiveness is considered to be 5-10/100 years of marriage. a combined usage of condom and spermicides such as nonoxynol-9 could thus elevate contraceptive effectiveness more than 20 times. a ...	1988	12316192
chlamydia trachomitis antigen in the cervix: prevalence in a student population.	the prevalence of chlamydia trachomatis was investigated over a two-year period in a population of women university students attending a student health clinic for routine pelvic examination or for a gynecologic complaint. direct immunofluorescence slide test was used to determine the presence of c. trachomatis. the overall prevalence rates for the two-years periods studied were 8.2% and 7.1%. when women who had symptoms of a urogenital infection were compared to women who were asymptomatic, it w ...	1988	21253068
[a comparative study of the incidence of gardnerella vaginalis in users of iud and oral contraceptives].	leukorrhea constitutes one of the most frequent complaints by women visiting out-patient gynecological clinics. the most common etiological agents are gardnerella vaginalis, trichomonas vaginalis, neisseria gonorrhoea and candida albicans. some authors have been able to verify an increased presence of certain pathogenic germs in the vaginal flora for users of contraceptive methods, e.g., for users of iud, kivijarvi et al. demonstrated a significantly increased presence of gardnerella vaginalis ...	1987	12282423
comparison of 2 g single dose of metronidazole, nimorazole and tinidazole in the treatment of vaginitis associated with gardnerella vaginalis.	vaginitis associated with the presence of gardnerella vaginalis (confirmed by culture) was treated either with metronidazole or with one of the two nitroimidazole derivatives; nimorazole or tinidazole, as a single oral 2 g dose. eighty-two patients were treated with metronidazole, 100 with nimorazole and 98 with tinidazole. the cure rates were 79%, 88% and 92% with metronidazole, nimorazole and tinidazole respectively. therefore we recommend a single dose of 2 g of any of these three drugs in th ...	1987	3494725
detection of gardnerella vaginalis in vaginal specimens by direct immunofluorescence.	the preparation of a fluorescein-labeled gardnerella vaginalis polyclonal antibody is described, and its usefulness is assessed for the detection of this microorganism in vaginal samples obtained from 263 women attending the gynecological department of a general hospital, 66 of whom harbored an intrauterine device. the direct immunofluorescence technique was positive for g. vaginalis in 21% of the specimens, whereas only 12.5% of the total bacteriological cultures were positive. the frequency wa ...	1987	3312289
the accuracy of colposcopically directed biopsy in diagnosis of cin.	during the last decade there has been an increasing interest in the use of the colposcope. a quality-control study for the evaluation and understanding of the limitations of cytology and colposcopy in our clinic is presented. the results in 132 patients are analysed and compared to previous reports from the literature. cytologic results correlated with the histological diagnosis only in 47% of the patients. colposcopically directed biopsies were accurate in 88.8% when the entire squamocolumnar j ...	1987	3493927
vaginitis: its diagnosis and treatment.		1987	3492484
quantitative studies on the vaginal flora of asymptomatic women and patients with vaginitis and vaginosis.	vaginal washings of 22 patients with vaginitis, 11 with vaginosis, and 12 healthy subjects were investigated quantitatively and qualitatively for aerobic and anaerobic bacteria and yeasts. gardnerella vaginalis was recovered from 9 of the vaginitis patients, 7 of the vaginosis patients, and 4 of the asymptomatic subjects. obligate anaerobes were found in 11 of the vaginitis patients, 4 of the vaginosis patients, and none of the control subjects. bacteroides bivius was the anaerobe most frequentl ...	1987	3314265
[gardnerella vaginalis infection. clinical aspects, diagnosis and therapy].	the gardnerella vaginalis-infection of the urogenital tract is of clinical importance in females and of epidemiological importance in males. females suffer from bacterial vaginosis, with a foul-smelling grey vaginal discharge with a ph of 5.0-5.5 which contains "clue cells", and from sepsis. the isolation and identification of g. vaginalis i necessary in man. if g. vaginalis-infection is suspected, simultaneous infections with further std-agents such as n. gonorrhoeae, c. trachomatis etc should ...	1987	3318083
the role of vaginal secretory immunoglobulin a, gardnerella vaginalis, anaerobes, and chlamydia trachomatis in postabortal pelvic inflammatory disease.	in a prospective study of 129 women undergoing induced first-trimester abortion, 14 (10.9%) contracted postabortal pelvic inflammatory disease (pid). samples of vaginal secretion for quantitation of secretory immunoglobulin a (siga) as well as isolates from cervix/urethra for the culture of anaerobes and aerobes, including bacteroides fragilis et melaninogenicus and gardnerella vaginalis, were obtained at the preoperative visit. two blood samples from each woman with postabortal pid were analyse ...	1987	3497518
gardnerella vaginalis in prepubertal girls.	a prospective study was established to determine the significance of the isolation of gardnerella vaginalis from the vagina in prepubertal children. two hundred fifty-six children were enrolled. group 1 consisted of 137 children who had been victims of sexual abuse; group 2, forty-eight children with genitourinary complaints and no history of sexual abuse; and group 3, seventy-one children with no genitourinary complaints and no history of sexual abuse. gardnerella vaginalis was isolated from 20 ...	1987	3497575
roxithromycin in nongonococcal urethritis.	this presentation is a summary of five different studies on the efficacy and tolerance of roxithromycin in the treatment of non-gonococcal genital infections. three of the studies were double-blind comparative and two were open studies. of the 924 out-patients whose data were analysed for clinical efficacy, 637 received treatment with roxithromycin 150 mg bd. the standard dose of roxithromycin, 150 mg bd for ten days, was compared with doxycycline 200 mg daily, lymecycline 300 mg bd and roxithro ...	1987	3323168
a review of the in-vitro activity of roxithromycin against genital pathogens.	the in-vitro activity of roxithromycin against neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, treponema pallidum, gardnerella vaginalis and haemophilus ducreyi is reviewed. roxithromycin demonstrated equivalent activity to erythromycin against n. gonorrhoeae, c. trachomatis, m. hominis, u. urealyticum, g. vaginalis and h. ducreyi. in a rabbit model for syphilis, potentially useful activity against t. pallidum has been demonstrated.	1987	3323169
single dose of ornidazole in the treatment of bacterial vaginosis.	bacterial vaginosis is the most common cause of excessive and foul smelling vaginal discharge. in the group of 24 women, the diagnosis was based on increased vaginal discharge, positive koh-test, and detection of clue cells in the wet smear. in the microbiological isolation, gardnerella vaginalis was also observed in 20 subjects and mixed anaerobic flora in all the 24 subjects. a single dose of 1.5 g of ornidazole was given. posttreatment control was performed 7 to 28 days later. gardnerella vag ...	1987	3499111
transmission rate of ureaplasma urealyticum, mycoplasma spp., gardnerella vaginalis, b-streptococci, candida spp. and chlamydia trachomatis from the mother to the newborn.	in a preliminary study of the transmission rate of ureaplasma urealyticum, mycoplasma species, gardnerella vaginalis, b-streptococci, candida species and chlamydia trachomatis from the mother to the newborn, swabs were taken from 45 parturients and their neonates and cultured by suitable methods. out of 30 parturients with a positive culture, 8 harboured more than one microorganism investigated. u. urealyticum was found in 11 newborn and all of them had a positive mother. candida spp. were found ...	1987	3324978
new developments in the etiology and pathogenesis of bacterial vaginosis.	bacterial vaginosis is unlike the "classic" sexually transmitted diseases. unlike cervical infection with chlamydia or salpingitis caused by n. gonorrhoeae, no single etiologic agent has been identified, and the organisms which are associated with infection have all been found as members of endogenous vaginal flora, with the possible exception of mobiluncus species. if, as we suspect, bv is due to interactions among various organisms found in the vagina during vaginal health, we must determine w ...	1987	3329809
haemagglutination and tissue culture adhesion of gardnerella vaginalis.	six strains of gardnerella vaginalis were studied to examine the adhesin-receptor mechanism involved in their attachment to human red blood cells and an epithelial tissue culture cell line (mccoy). the adhesins involved in the attachment of the bacteria to each of these cells were proteinaceous but showed marked differences after various chemical or physical treatments, indicating that separate adhesins were present. haemagglutinating strains were more hydrophobic than tissue-culture-adherent st ...	1987	3502136
incidence of motile, curved anaerobic rods (mobiluncus species) in vaginal secretions.	aerobic and anaerobic cultures as well as a gram stain and wet mount preparation were made of vaginal swabs taken from various groups of women including those with vaginal discharge. the bacteria commonly found in cultures were lactobacilli, coryneforms, staphylococcus epidermidis and facultative streptococci. anaerobes were isolated from 75% (475 of 632) of specimens. the incidence of trichomonas vaginalis, candida species, gardnerella vaginalis and mobiluncus species in the five groups of wome ...	1987	3502614
[cervico-vaginal pathogens and contraception: microbiological observations].	the authors relate the results about 172 vaginal and cervical swabs, in women with or without oral/local (iud) contraception, with or without vaginosis/vaginitis. gardnerella vaginalis was always prevalent; the authors observed an high correlation between cervical iud and vaginal gardnerella, more than iud and cervical chlamydia trachomatis. bacterial associations in gardnerella vaginalis (mobiluncus, obligate anaerobes) are related; incidence of candida and trichomonas vaginalis are reported; c ...	1987	3508298
therapy of amine-vaginitis by a single dose of ornidazole (tiberal); serum and tissue (vagina) concentrations of ornidazole after vaginal or rectal application.		1987	3509939
single-dose therapy for genitourinary infections.	single-dose therapy for selected genitourinary tract infections is an effective alternative to multiple-dose regimens. candidal vulvovaginitis and trichomonal vaginitis may be routinely treated with single-dose regimens. with acute cystitis, candidates for single-dose therapy include patients who have a short duration of symptoms and are likely to comply with follow-up.	1987	3500627
in vitro adhesiveness and biotype of gardnerella vaginalis strains in relation to the occurrence of clue cells in vaginal discharges.	haemagglutination and tissue culture adherence tests using a mccoy cell line were used to examine the adherence characteristics of 105 strains of gardnerella vaginalis. each strain represented one isolate per patient. for each patient, a direct smear of vaginal discharge was examined for clue cells. the relation between in vitro adherence and the presence of clue cells was examined. there seemed to be no appreciable relation between the presence of clue cells in smears and the haemagglutinating ...	1987	3493202
treatment of common genital infections in adolescents.		1987	3546225
extra-vaginal infection caused by gardnerella vaginalis.		1987	3493915
the acute urethral syndrome in routine practice.	midstream samples of urine from 185 acutely dysuric women and 89 symptom-free controls were screened according to the modified criteria of kass by both conventional and microaerophilic culture. among the 185 symptomatic women, coliform bacilli were isolated from 125 (67.5%) and in 45 (36%) of the latter the concentration of these organisms in the urine was less than 10(8)/l. fastidious organisms were isolated in pure and mixed cultures from 25 (13.4%) of the 185 patients and from 4 (4.5%) of 89 ...	1987	3494789
susceptibility of gardnerella vaginalis to metronidazole, its bioactive metabolites, and tinidazole.	the susceptibilities of 510 clinical isolates of gardnerella vaginalis to metronidazole, its principal oxidative metabolites, and tinidazole were determined by an agar dilution method. the hydroxy metabolite was the most active, with an mic90 value (minimum concentration that inhibited 90% of the strains) of 1.12 mg/l (5.51 mumol/l). tinidazole and metronidazole were somewhat less active, with mic90s of 4.09 mg/l (23.9 mumol/l) and 4.44 mg/l (18.0 mumol/l), respectively. the acid metabolite was ...	1987	3495168
treatment of clue cell-positive discharge with 200 mg povidone-iodine pessaries. a double-blind and placebo-controlled trial.	in a double-blind randomized study we evaluated the efficacy of povidone-iodine in the treatment of clue cell-positive discharge (ccpd). vaginal pessaries (200 mg povidone-iodine or placebo) were taken twice daily for five consecutive days. although the regimen was reasonably well accepted, 'messiness' was reported by 13 of 33 women (39%). of 44 women enrolled, treatment efficacy was evaluated in 28 women who had both follow-up visits. there was no significant difference in the efficacy of povid ...	1987	3556257
susceptibility of mobiluncus species to 23 antimicrobial agents and 15 other compounds.	the susceptibility of 12 strains of mobiluncus curtisii and 10 strains of m. mulieris to 23 antimicrobial agents and 15 other compounds was determined. all strains were susceptible to chloramphenicol, clindamycin, rifampin, tobramycin, vancomycin, virginiamycin, and all beta-lactam antibiotics tested, including imipenem. one strain of m. mulieris was resistant to erythromycin and josamycin. all were resistant to colistin, cycloserine, nalidixic acid, and neomycin. tetracycline had variable activ ...	1987	3566250
infection with multiple sexually transmitted agents.	a patient named as a contact by an individual with gonorrhea and syphilis was found to be an asymptomatic carrier of seven organisms known to be transmitted sexually. cultures were positive for neisseria gonorrhoeae, chlamydia trachomatis, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and herpes simplex virus. trichomonas vaginalis was detected by a wet preparation and the papanicolaou smear. the cervix was found to have an ulcer on the posterior lip that yielded herpes simp ...	1987	3573062
controlled evaluation of modified radiometric blood culture medium supplemented with gelatin for detection of bacteremia and fungemia.	although the addition of 1.2% gelatin to broth blood culture media containing sodium polyanetholesulfonate has been shown to enhance detection of certain bacteria, including neisseria meningitidis, n. gonorrhoeae, peptostreptococcus anaerobius, and gardnerella vaginalis, the effect of such supplementation on the detection of other microorganisms causing bacteremia and fungemia is not known. therefore, we studied bactec 6b medium with and without gelatin in 6,833 paired comparisons to examine the ...	1987	3624437
[bacterial vaginitis within the scope of gynecologic consultation].	examinations about bacterial vaginosis have been done in 384 fertile women according the following diagnostic criteria: homogenous gray flour, typical fish smelling, clue cells and ph of 5 in vaginal content. clue cells could be detected in 233 (60.6 per cent) women. bacterial vaginosis with three of the above mentioned criteria could be found in 40.4 per cent. the cure rate following oral metronidazole therapy (twice daily 500 mg metronidazole for 5 days) was 75.2 per cent. in cases with therap ...	1987	3495945
antimicrobial effects of niridazole on gardnerella vaginalis.	niridazole, a nitrothiazole derivative, demonstrated powerful antimicrobial activity against 510 clinical isolates of gardnerella vaginalis tested. mic's ranged from 0.002 to 1.0 mg/l with mic50 and mic90 values of 0.02 and 0.067 mg/l respectively.	1987	3496279
microbiologic and serologic studies of gardnerella vaginalis in intra-amniotic infection.	our objective was to investigate the role of gardnerella vaginalis in intra-amniotic infection by use of comparative, quantitative cultures on selective media and by detection of maternal antibody response. amniotic fluid was collected from patients with intra-amniotic infection and from matched control women. in addition to media for aerobes, anaerobes, and mycoplasmas, we used v agar-selective (remel, lenexa, ks) to isolate g vaginalis. acute and convalescent maternal sera were collected and a ...	1987	3496566
development and evaluation of scheme for serotyping gardnerella vaginalis.	antibodies to gardnerella vaginalis were raised in rabbits. nine antisera that reacted with their immunising strains, but not with the remaining eight strains, were used to develop a serotyping scheme. a dot blotting technique was used, and complexes of antigen and antibody were visualised using anti-rabbit immunoglobulin linked to alkaline phosphatase. of 91 clinical isolates used to evaluate the scheme, 79 (87%) were typable and 52 (57%) reacted with only a single antiserum. the antigens expre ...	1987	3497087
the role of benzydamine in the topical treatment of the so-called non-specific vaginitis.	the authors report the preliminary results of their investigation of the efficacy of benzydamine in the treatment of the so-called non-specific vaginitis. an initial in vitro study to test its bacteriostatic and/or bactericidal activity on gardnerella vaginalis showed that the drug has high activity even at the lowest concentrations, and completely inhibits this micro-organism at 1000 micrograms/ml which is the usual concentration employed in therapy. the first pilot study performed in vivo on 1 ...	1987	3497131
non-specific vaginitis or vaginitis of undetermined aetiology.	vaginitis is a complex syndrome that is probably the most common outpatient disease seen by the gynaecologist. the specific aetiologies of vaginitis are many. one of the most common entities, however, is "non-specific vaginitis" which can be subdivided into: gardnerella vaginitis, anaerobic vaginosis, and vaginitis of undetermined aetiology. the role of gardnerella as a causative agent for vaginitis has been studied in depth but its specific role remains controversial. anaerobic vaginosis can be ...	1987	3497132
syphilitic patients with urogenital infection caused by chlamydia trachomatis, ureaplasma urealyticum and gardnerella vaginalis.		1987	3497827
identifying vaginitis in general practice.	clinicians conducted a study of 154 women who presented themselves at a health center of the university of wales college of medicine with symptoms of vaginitis. a nurse examined the vagina with a speculum to note the appearance of the cervix, the color and amount of discharge, and the presence of odor and inquired about soreness during the examination. the nurse took 3 endocervical swabs and 2 high vaginal swabs. upon microscopic examination, any vaginal discharge with epithelial cells stippl ...	1987	3498151
non-specific (anaerobic) vaginitis: relevance of clinical and laboratory studies in a practice population.	non-specific vaginitis is a commonly diagnosed condition defined in a similar manner by most authors. although assumed to be of infective aetiology, no single organism has yet been accepted as the primary agent. this syndrome was studied in two groups of women presenting to general practitioners or attending a family planning clinic. the two groups were of similar ages and had similar markers of sexual activity. of the 173 women studied, 90 had symptoms. of the symptomatic women 9.5% could be ca ...	1987	3499507
clinical prediction of gardnerella vaginalis in general practice.	in a study of 162 women with vaginal symptoms the clinical features of increased discharge, yellow discharge, 'high cheese' odour and ph greater than 5 were statistically strongly associated with the presence of gardnerella vaginalis, confirmed by microbiological culture. the sensitivities and specificities of these clinical tests, although not as high as those of previously described sideroom tests using the amine test and microscopy for 'clue cells' nevertheless allow the clinician to predict ...	1987	3499508
gardnerella vaginalis in the male upper genital tract: a possible source of reinfection of the female partner.	we describe a case of gardnerella vaginalis colonization of the upper genital tract of the male partner of a woman with recurring bacterial vaginosis. g. vaginalis could not be cultured from the urethra but was cultured from semen. after treatment of the male partner with metronidazole, the woman had no more relapses of bacterial vaginosis.	1987	3497456
isolation of gardnerella vaginalis in pure culture from the uterine cavity of patients with irregular bleedings.	hysterectomy was performed in three patients because of persistent irregular vaginal bleeding. before the operation samples were taken from the cervical os for cultivation of gardnerella vaginalis, yeasts, viruses, chlamydia trachomatis, and aerobic and anaerobic bacteria. immediately after the operation, the uterus was opened under sterile conditions and samples obtained from the isthmus and fundus of the uterine cavity were examined microbiologically. in all three patients g. vaginalis was gro ...	1987	3500741
gardnerella vaginalis chorioamnionitis: a report of two cases and a review of the pathogenic role of g. vaginalis in obstetrics.	two patients with chorioamnionitis due to gardnerella vaginalis are described. institution of tocolytic therapy for preterm labor is associated with maternal complications of septic hypotension and pulmonary edema in one patient. diagnostic modalities, specifically culturing techniques, are discussed, as well as suspected pathophysiologic mechanisms.	1987	3501357
bacterial vaginosis: microbiological and clinical findings.	a prospective study was performed involving 101 women who consecutively attended a primary health care unit for complaints of genital malodour and/or abnormal vaginal discharge. bacterial vaginosis was diagnosed in 34 women on the basis of four diagnostic criteria: vaginal ph greater than 4.7, homogeneous vaginal discharge, a positive amine test and clue cells. the sensitivity of these criteria was greater than 90% except for homogeneous discharge (82%). their specificity was greater than 90% ex ...	1987	3501755
clinical evaluation of the vitek neisseria-haemophilus identification card.	a clinical evaluation of the vitek neisseria-haemophilus identification (nhi) card (vitek systems, inc., hazelwood, mo.) was performed with 480 clinical isolates and stock strains of neisseria spp., haemophilus spp., and other fastidious microorganisms included in the data base of the system. identifications obtained with the nhi card were compared with those determined by conventional methods. the card identified 83.2% of 244 neisseria spp. and branhamella catarrhalis, 54.9% of 164 haemophilus ...	1987	3539996
evaluation of enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis in genital tract specimens.	an enzyme immunoassay (chlamydiazyme) for detecting chlamydia trachomatis was evaluated on genital specimens from 96 men and 272 women attending a clinic for sexually transmitted diseases (std clinic). compared with a direct immunofluorescence test for chlamydial elementary bodies, the enzyme immunoassay had a sensitivity of 58% on specimens from men, a specificity of 90%, a positive predictive value of 93%, and a negative predictive value of 88%; the assay had a sensitivity of 67% on specimens ...	1987	3546397
comparison of radiometric and gas capture systems for blood cultures.		1987	3546400
sexually transmitted diseases.		1987	3039364
significant bacteremia associated with replacement of intrauterine contraceptive device.	blood culture samples were taken from 23 women at different stages when an intrauterine contraceptive device was replaced. transient bacteremia resulting from vaginal organisms was found in 13% of women 4 to 6 minutes after insertion of the new device. previous reports have failed to demonstrate bacteremia associated with either first insertion or removal of intrauterine contraceptive device. our results show that replacement of an intrauterine contraceptive device, a more traumatic procedure, c ...	1987	3826221
controlled evaluation of trypticase soy broth with and without gelatin and yeast extract in the detection of bacteremia and fungemia.	the addition of gelatin to blood culture media has been suggested to prevent the inhibition of neisseria meningitidis, neisseria gonorrhoeae, gardnerella vaginalis, and peptostreptococcus anaerobius that is caused by sodium polyanetholsulfonate. to determine the effect of such supplementation on the overall yield of microorganisms, we compared the yield and speed of detection of clinically important microorganisms from 5422 paired 10-ml samples of blood cultured in trypticase soy broth (tsb) con ...	1987	2831009
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.	the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...	1987	3030109
the midcycle cervical microbial flora as studied by the weighed-swab method, and its possible correlation with results of sperm cervical mucus penetration tests.	infertile couples undergoing routine investigation for infertility were randomly selected for the study. a quantitative method using weighed swabs was found satisfactory for the study of microbial flora in the midcycle cervical mucus. in 6 of the 20 women, the midcycle cervical mucus was hostile in the sperm cervical mucus penetration tests. the total bacterial counts and the number of species (average, 2.5) isolated from the hostile mucus were significantly greater than those from the receptive ...	1987	3109958
[the microbiological characteristics of bacterial vaginosis].		1987	3154365
cervical mosaic and an integrated pathophysiological approach to early cervical neoplasia.	the increasing concern for the early detection and treatment of cervical intraepithelial neoplasia (cin) set up the basis for this study, which also considers some epidemiological factors which seem to be related to the genesis of an abnormal uterine cervical colposcopic pattern, the mosaic pattern. we have observed that women with a mosaic pattern show an increasing frequency of premalignant and/or malignant histologic changes up to 40 years of age which then falls abruptly. sterility and hirsu ...	1987	2824702
[bacteriologic studies in premature rupture of fetal membranes and correlation with the clinical aspects of chorioamnionitis and the amnion infection syndrome].	one of the major complications following premature rupture of the membranes (prom) is ascending infection. in this aspect the bacteria of the vaginal flora play a major part. bacterial spectra of a group with prom and another control group with punctual rupture of the membranes are comparatively analysed. this analysis is based on swabs taken from vagina and newborns. data are interpreted in correlation to clinical picture of chorioamnionitis and amnion infection syndrome (ais). twelve newborns ...	1987	3327326
the in-vitro activity of roxithromycin, a new macrolide antibiotic, in comparison with that of erythromycin.	a study was made of the in-vitro activity of roxithromycin in comparison with that of erythromycin on selected recent clinical isolates of a wide range of organisms. minimum inhibitory concentrations (mics) were determined by an agar dilution method with an inoculum of 10(4) cfu. minimum bactericidal concentrations (mbcs) and the effect of ph were determined by a broth dilution method on selected strains. in general the in-vitro activity of roxithromycin mirrored that of erythromycin, but it was ...	1987	3428135
comparative in vitro activity of the two new oral cephalosporin metabolites ro 19-5247 and ro 15-8074.	a total of 629 clinical strains of gram positive and gram negative bacteria were tested for their susceptibility to ro 19-5247, ro 15-8074, and other antimicrobial agents. both ro 19-5247 and ro 15-8074 had good activity against strains of enterobacteriaceae; however, resistance was found among some strains of enterobacter, citrobacter, klebsiella and morganella spp. both compounds showed moderate to poor active against acinetobacter spp., pseudomonas aeruginosa, staphylococci and streptococcus ...	1987	3436316
[gardnerella vaginalis in nonspecific vaginitis].		1987	3296818
[diagnosis of flat condylomas of the uterine cervix: problems with associated infections and differential diagnosis].		1987	2829756
pili on gardnerella vaginalis studied by electronmicroscopy.	fourteen recently isolated strains and two laboratory strains of gardnerella vaginalis were examined by electronmicroscopy for the presence of pili. all strains isolated recently from both men and women were heavily pilated. in contrast only a few pili were seen on organisms of the two laboratory strains, with many of the organisms having no pili. the importance of multiple subculture in this loss was supported by the observation that the degree of pilation of one freshly isolated strain decreas ...	1987	2884322
salpingitis; aspects of diagnosis and etiology: a 4-year study from a swedish capital hospital.	359 patients underwent laparoscopy to verify the diagnosis of salpingitis. pelvic inflammatory disease (pid) was found in 187 (52%) cases. laparoscopy revealed normal conditions in 136 (37%) cases. other diseases were diagnosed in 36 (10%) cases. bacteriological cultures from the fimbrial lumen were positive in 24% of the pid cases. chlamydia trachomatis (ct) was detected in 12%, bacteroides species (bs) in 5%, actinomyces israelii (ai) in 3%, gardnerella vaginalis (gv) in 2%, neisseria gonorrho ...	1987	2951285
preterm labor associated with subclinical amniotic fluid infection and with bacterial vaginosis.	maternal genital infection, particularly subclinical amniotic fluid infection, may cause preterm labor and a premature delivery. the prevalence of subclinical amniotic fluid infection was studied in 54 consecutive afebrile women in preterm labor with singleton gestations and intact fetal membranes. microorganisms were recovered from the amniotic fluid by transabdominal amniocentesis in 13 (24%) of 54 patients. bacteria or candida albicans were recovered from six (11%), and genital mycoplasmas fr ...	1986	3003634
contribution of gardnerella vaginalis to vaginitis in a general practice.	in a study of 154 adult women who presented to their general practitioner with vaginal symptoms 30 (20%) had gardnerella vaginalis on its own and 51 (33%) had g vaginalis in combination with anaerobes or known pathogens. thirty one (20%) patients were culture negative. those who were culture negative had fewer symptoms and signs of vaginitis than those with g vaginalis alone or g vaginalis plus anaerobes. those with known pathogens had more symptoms and signs than those with g vaginalis alone or ...	1986	3013357
in vitro activity of difloxacin hydrochloride (a-56619), a-56620, and cefixime (cl 284,635; fk 027) against selected genital pathogens.	management of sexually transmitted diseases is facilitated by having antimicrobial agents with activity against all of the major genital pathogens. newer quinolones show promise of being active against neisseria gonorrhoeae and chlamydia trachomatis. two quinolones, difloxacin (a-56619) and a-56620, and an oral cephalosporin, cefixime (cl 284,635; fk 027), were evaluated in vitro. all three were highly active against 400 isolates of n. gonorrhoeae, including penicillinase-producing n. gonorrhoea ...	1986	3098163
the in-vitro activity of ci-934 compared with that of other new 4-quinolones and nalidixic acid.	the in-vitro activity of ci-934, a new 4-quinolone compound, was compared with that of the other new 4-quinolones, enoxacin and ciprofloxacin, and also with that of nalidixic acid. ci-934 was more active than any of the other 4-quinolones tested against gram-positive aerobic organisms including staphylococcus aureus (mics 0.06-0.25 mg/l), beta-haemolytic streptococci (mics 0.12-0.5 mg/l), streptococcus pneumoniae (mics 0.25-0.5 ml/l), viridans streptococci (mics 0.06-0.5 mg/l) and most enterococ ...	1986	3463557
the comparative in-vitro activity of eight newer quinolones and nalidixic acid.	the in-vitro antibacterial activity of nalidixic acid and the 4-quinolones, ciprofloxacin, norfloxacin, enoxacin, ofloxacin, pefloxacin, a-56619, a-56620 and ci-934 was assessed by determination of mics. the 4-quinolones were all highly active against most isolates of enterobacteriaceae, including nalidixic acid-resistant strains. ciprofloxacin (mics 0.002-2 mg/l) was the most active and a-56619 (mics 0.008-32 mg/l) was the least active. a-56619, a-56620, ofloxacin, ciprofloxacin and ci-934 were ...	1986	3468100
high-level tetracycline resistance in neisseria gonorrhoeae is result of acquisition of streptococcal tetm determinant.	recently, strains of neisseria gonorrhoeae have been isolated which are highly resistant to tetracycline (mics of 16 to 64 micrograms/ml). this resistance was due to the acquisition of the resistance determinant tetm, a transposon-borne determinant initially found in the genus streptococcus and more recently in mycoplasma hominis, ureaplasma urealyticum, and gardnerella vaginalis. in n. gonorrhoeae, the tetm determinant was located on a 25.2-megadalton plasmid. this plasmid arose from the insert ...	1986	3099640
the treatment of gardnerella vaginalis infection in general practice.		1986	3485279
etiology of cervical inflammation.	we studied the relationships of selected microbial, clinical, demographic, and behavioral variables to mucopurulent cervicitis in two clinical settings, a sexually transmitted disease clinic and a student health clinic. from each clinic, we studied a group of women referred for suspected mucopurulent cervicitis and a representative sample of other women attending the clinic. after the women were stratified by patient group and summary odds ratios for all groups were obtained, mucopurulent cervic ...	1986	3485379
tioconazole 2% cream in the treatment of trichomonas vaginalis or mixed vaginal infections.	twenty patients with documented t. vaginalis infections, six of whom were simultaneously infected with c. albicans and one with g. vaginalis, were treated topically (intravaginally) once daily with 5 g of 2% tioconazole vaginal cream (trosyd, pfizer) for 3 consecutive days in an open, non-comparative study. at the first follow-up visit (about 7 days post-treatment), 95% (19/20) of the patients were cured; 95% (18/19) of patients remained cured with respect to the baseline infection at the long-t ...	1986	3485546
gardnerella vaginalis and mosaic colposcopic pattern of the cervix: casual or causal association?	we made a retrospective study of the flora found in the vaginal smears of sexually active women who presented with a mosaic colposcopic pattern of the uterine cervix in an attempt to study some of its epidemiologic factors. of the 195 cervicovaginal cytologies of patients with mosaic, 61 revealed an abnormal flora (31.2%) while of the 9,856 patients without mosaic, only 2,234 cytologies had an abnormal flora (22.7%) demonstrating a statistically significant higher frequency (p less than 0.0001) ...	1986	3485553
gardnerella vaginitis--a guide to identification and management for the practitioner.		1986	3485708
in vitro activity of a-56619 (difloxacin), a-56620, and other new quinolone antimicrobial agents against genital pathogens.	the in vitro activities of two new carboxyquinolones, a-56619 (difloxacin) and a-56620, were compared with those of ciprofloxacin, norfloxacin, and ofloxacin against genital tract pathogens. all the quinolones were highly active against neisseria gonorrhoeae. a-56619 had the lowest mics against chlamydia trachomatis (mic range, 0.125 to 0.25 micrograms/ml) and haemophilus ducreyi (mic for 90% of isolates tested, 0.1 micrograms/ml).	1986	3101590
[symptomatic and asymptomatic vaginitis. microbiological, clinical and therapeutic aspects].		1986	3488870
frequency and epidemiologic associations of different types of vaginitis in symptomatic women in greece.		1986	3489617
incidence of gardnerella vaginalis in non-specific vaginitis.		1986	3489675
prevalence of nonspecific vaginitis and other cervicovaginal infections during the third trimester of pregnancy.	nonspecific vaginitis (nsv) is a clinical syndrome characterized by a decreased concentration of normal acidophilic döderlein flora in the vagina and increased concentration of nonacidophilic microorganisms, such as gardnerella vaginalis, anaerobic bacteria, and mycoplasmas. since several nsv-associated organisms are associated with complications of pregnancy, we studied the prevalence of nsv among 104 consecutive women during the third trimester of pregnancy. overall, 14 women (14%) had nsv by ...	1986	3082020
sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls.	sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ...	1986	3083395